PRIMER_OPT_SIZE=20
PRIMER_PICK_INTERNAL_OLIGO=1
P3_COMMENT=Used to have: PRIMER_INTERNAL_OLIGO_OFFSET 4 (not used)
PRIMER_INTERNAL_MAX_SELF_END=8
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=25
PRIMER_OPT_TM=60.0
PRIMER_MIN_TM=57.0
PRIMER_MAX_TM=63.0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_MAX_POLY_X=100
PRIMER_SALT_MONOVALENT=50.0
PRIMER_DNA_CONC=50.0
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=8
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=8
P3_COMMENT= Used to have: PRIMER_NUM_TERMINATORS_0 (not used)
P3_COMMENT= Used to have: PRIMER_REST_SITES_" " (not used)
P3_COMMENT= Used to have: PRIMER_ALLOW_REST_SITE_0 (not used)
PRIMER_PRODUCT_SIZE_RANGE=75-100 100-125 125-150 150-175 175-200 200-225
SEQUENCE_ID=MH1000
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTNAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
SEQUENCE_INCLUDED_REGION=36,342
P3_COMMENT=CL is TTACTGGAAGAGAGGGTCATG
P3_COMMENT=CR is AGGGAAACATCTGCAAGGTT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.150428
PRIMER_LEFT_0_PENALTY=0.742026
PRIMER_RIGHT_0_PENALTY=0.408401
PRIMER_INTERNAL_0_PENALTY=0.017846
PRIMER_LEFT_0_SEQUENCE=ACTGGAAGAGAGGGTCATGC
PRIMER_RIGHT_0_SEQUENCE=CATTCAGTGCGTCTCCTCCT
PRIMER_INTERNAL_0_SEQUENCE=AAGTTTGCAAAGGCAGGCTA
PRIMER_LEFT_0=69,20
PRIMER_RIGHT_0=143,20
PRIMER_INTERNAL_0=104,20
PRIMER_LEFT_0_TM=59.258
PRIMER_RIGHT_0_TM=60.408
PRIMER_INTERNAL_0_TM=60.018
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=1.186960
PRIMER_LEFT_1_PENALTY=0.339231
PRIMER_RIGHT_1_PENALTY=0.847729
PRIMER_INTERNAL_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_RIGHT_1_SEQUENCE=CTTACCATGGCATTCAGTGC
PRIMER_INTERNAL_1_SEQUENCE=GTTTGCAAAGGCAGGCTAAG
PRIMER_LEFT_1=72,20
PRIMER_RIGHT_1=153,20
PRIMER_INTERNAL_1=106,20
PRIMER_LEFT_1_TM=59.661
PRIMER_RIGHT_1_TM=59.152
PRIMER_INTERNAL_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=1.526945
PRIMER_LEFT_2_PENALTY=1.509099
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_INTERNAL_2_PENALTY=0.339231
PRIMER_LEFT_2_SEQUENCE=GCATCAGTGAGTACAGCATGC
PRIMER_RIGHT_2_SEQUENCE=AGCCTGCCTTTGCAAACTTA
PRIMER_INTERNAL_2_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT_2=46,21
PRIMER_RIGHT_2=122,20
PRIMER_INTERNAL_2=72,20
PRIMER_LEFT_2_TM=59.491
PRIMER_RIGHT_2_TM=60.018
PRIMER_INTERNAL_2_TM=59.661
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=1.526945
PRIMER_LEFT_3_PENALTY=1.509099
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_INTERNAL_3_PENALTY=0.339231
PRIMER_LEFT_3_SEQUENCE=GCATCAGTGAGTACAGCATGC
PRIMER_RIGHT_3_SEQUENCE=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_3_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT_3=46,21
PRIMER_RIGHT_3=123,20
PRIMER_INTERNAL_3=72,20
PRIMER_LEFT_3_TM=59.491
PRIMER_RIGHT_3_TM=60.018
PRIMER_INTERNAL_3_TM=59.661
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=1.526945
PRIMER_LEFT_4_PENALTY=1.509099
PRIMER_RIGHT_4_PENALTY=0.017846
PRIMER_INTERNAL_4_PENALTY=0.339231
PRIMER_LEFT_4_SEQUENCE=GCATCAGTGAGTACAGCATGC
PRIMER_RIGHT_4_SEQUENCE=TTAGCCTGCCTTTGCAAACT
PRIMER_INTERNAL_4_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT_4=46,21
PRIMER_RIGHT_4=124,20
PRIMER_INTERNAL_4=72,20
PRIMER_LEFT_4_TM=59.491
PRIMER_RIGHT_4_TM=60.018
PRIMER_INTERNAL_4_TM=59.661
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH101
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTNCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG
SEQUENCE_INCLUDED_REGION=35,254
P3_COMMENT=CL is CCTCTGATCATCTTCTGATCC
P3_COMMENT=CR is GTAAAATGGATAGATTCGGACC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.588776
PRIMER_LEFT_0_PENALTY=1.201261
PRIMER_RIGHT_0_PENALTY=2.387515
PRIMER_INTERNAL_0_PENALTY=8.314301
PRIMER_LEFT_0_SEQUENCE=TGAACCCATTCTATCAGGTGG
PRIMER_RIGHT_0_SEQUENCE=TGGATAGATTCGGACCTTTG
PRIMER_INTERNAL_0_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA
PRIMER_LEFT_0=201,21
PRIMER_RIGHT_0=278,20
PRIMER_INTERNAL_0=232,27
PRIMER_LEFT_0_TM=59.799
PRIMER_RIGHT_0_TM=57.612
PRIMER_INTERNAL_0_TM=58.686
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=29.630
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=3.868652
PRIMER_LEFT_1_PENALTY=1.201261
PRIMER_RIGHT_1_PENALTY=2.667391
PRIMER_INTERNAL_1_PENALTY=8.314301
PRIMER_LEFT_1_SEQUENCE=TGAACCCATTCTATCAGGTGG
PRIMER_RIGHT_1_SEQUENCE=AATGGATAGATTCGGACCTTTG
PRIMER_INTERNAL_1_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA
PRIMER_LEFT_1=201,21
PRIMER_RIGHT_1=280,22
PRIMER_INTERNAL_1=232,27
PRIMER_LEFT_1_TM=59.799
PRIMER_RIGHT_1_TM=59.333
PRIMER_INTERNAL_1_TM=58.686
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_INTERNAL_1_GC_PERCENT=29.630
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=4.195249
PRIMER_LEFT_2_PENALTY=1.201261
PRIMER_RIGHT_2_PENALTY=2.993987
PRIMER_INTERNAL_2_PENALTY=8.314301
PRIMER_LEFT_2_SEQUENCE=TGAACCCATTCTATCAGGTGG
PRIMER_RIGHT_2_SEQUENCE=ATGGATAGATTCGGACCTTTG
PRIMER_INTERNAL_2_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA
PRIMER_LEFT_2=201,21
PRIMER_RIGHT_2=279,21
PRIMER_INTERNAL_2=232,27
PRIMER_LEFT_2_TM=59.799
PRIMER_RIGHT_2_TM=58.006
PRIMER_INTERNAL_2_TM=58.686
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=29.630
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=4.414739
PRIMER_LEFT_3_PENALTY=0.345214
PRIMER_RIGHT_3_PENALTY=4.069526
PRIMER_INTERNAL_3_PENALTY=4.276719
PRIMER_LEFT_3_SEQUENCE=TCTTCTGATCCAAGACCCGT
PRIMER_RIGHT_3_SEQUENCE=TCTGGATTCTCTGATGATTATGGA
PRIMER_INTERNAL_3_SEQUENCE=CATGTACCCTTCTACAAGCTTTGA
PRIMER_LEFT_3=45,20
PRIMER_RIGHT_3=134,24
PRIMER_INTERNAL_3=85,24
PRIMER_LEFT_3_TM=59.655
PRIMER_RIGHT_3_TM=59.930
PRIMER_INTERNAL_3_TM=59.723
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=37.500
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=4.715860
PRIMER_LEFT_4_PENALTY=0.646334
PRIMER_RIGHT_4_PENALTY=4.069526
PRIMER_INTERNAL_4_PENALTY=4.276719
PRIMER_LEFT_4_SEQUENCE=CTTCTGATCCAAGACCCGTG
PRIMER_RIGHT_4_SEQUENCE=TCTGGATTCTCTGATGATTATGGA
PRIMER_INTERNAL_4_SEQUENCE=CATGTACCCTTCTACAAGCTTTGA
PRIMER_LEFT_4=46,20
PRIMER_RIGHT_4=134,24
PRIMER_INTERNAL_4=85,24
PRIMER_LEFT_4_TM=60.646
PRIMER_RIGHT_4_TM=59.930
PRIMER_INTERNAL_4_TM=59.723
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=37.500
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH1013
SEQUENCE_TEMPLATE=CTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGTGGAGAAGCCTGGTCAGGGAGAGTATACAGGGATGGGGACTGCATGTATGGTTTGGAGTNCAGGAGGGAGAAAGGTGGTTCTTGTGGGAAGAGGTCATCTTCTAGGGCTGAGTTTGGCATTCTTAAGTTTTAACTCTGGGCCTGATATTCCCTGTTAGCCATCTCCAGGAAGAAGCATTTCATGACTCATATTTTTAACATGACTAATGTTACCTAGAACCACGTATCCTCC
SEQUENCE_INCLUDED_REGION=33,235
P3_COMMENT=CL is CTGGTCAGGGAGAGTATACAGG
P3_COMMENT=CR is GTGGTTCTAGGTAACATTAGTCATG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.244226
PRIMER_LEFT_0_PENALTY=0.080568
PRIMER_RIGHT_0_PENALTY=0.163658
PRIMER_INTERNAL_0_PENALTY=0.056936
PRIMER_LEFT_0_SEQUENCE=ATGGGGACTGCATGTATGGT
PRIMER_RIGHT_0_SEQUENCE=ATGCCAAACTCAGCCCTAGA
PRIMER_INTERNAL_0_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
PRIMER_LEFT_0=68,20
PRIMER_RIGHT_0=155,20
PRIMER_INTERNAL_0=105,20
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=59.836
PRIMER_INTERNAL_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.8000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.266806
PRIMER_LEFT_1_PENALTY=0.056936
PRIMER_RIGHT_1_PENALTY=0.209870
PRIMER_INTERNAL_1_PENALTY=0.163658
PRIMER_LEFT_1_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
PRIMER_RIGHT_1_SEQUENCE=CTGGAGATGGCTAACAGGGA
PRIMER_INTERNAL_1_SEQUENCE=TCTAGGGCTGAGTTTGGCAT
PRIMER_LEFT_1=105,20
PRIMER_RIGHT_1=203,20
PRIMER_INTERNAL_1=136,20
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.210
PRIMER_INTERNAL_1_TM=59.836
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=0.294074
PRIMER_LEFT_2_PENALTY=0.080568
PRIMER_RIGHT_2_PENALTY=0.213506
PRIMER_INTERNAL_2_PENALTY=0.056936
PRIMER_LEFT_2_SEQUENCE=ATGGGGACTGCATGTATGGT
PRIMER_RIGHT_2_SEQUENCE=GAATGCCAAACTCAGCCCTA
PRIMER_INTERNAL_2_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
PRIMER_LEFT_2=68,20
PRIMER_RIGHT_2=157,20
PRIMER_INTERNAL_2=105,20
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.214
PRIMER_INTERNAL_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=0.350010
PRIMER_LEFT_3_PENALTY=0.056936
PRIMER_RIGHT_3_PENALTY=0.293074
PRIMER_INTERNAL_3_PENALTY=0.163658
PRIMER_LEFT_3_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
PRIMER_RIGHT_3_SEQUENCE=GGGAATATCAGGCCCAGAGT
PRIMER_INTERNAL_3_SEQUENCE=TCTAGGGCTGAGTTTGGCAT
PRIMER_LEFT_3=105,20
PRIMER_RIGHT_3=187,20
PRIMER_INTERNAL_3=136,20
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=60.293
PRIMER_INTERNAL_3_TM=59.836
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=0.390277
PRIMER_LEFT_4_PENALTY=0.226618
PRIMER_RIGHT_4_PENALTY=0.163658
PRIMER_INTERNAL_4_PENALTY=0.056936
PRIMER_LEFT_4_SEQUENCE=TATACAGGGATGGGGACTGC
PRIMER_RIGHT_4_SEQUENCE=ATGCCAAACTCAGCCCTAGA
PRIMER_INTERNAL_4_SEQUENCE=GAAAGGTGGTTCTTGTGGGA
PRIMER_LEFT_4=59,20
PRIMER_RIGHT_4=155,20
PRIMER_INTERNAL_4=105,20
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.836
PRIMER_INTERNAL_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=97
=
SEQUENCE_ID=MH1017
SEQUENCE_TEMPLATE=TGTTTCATGCTGCAGGTCGACTCTAGAGGATCCCTACATAAACAGCAGCGAAAGGAGAAAGCTTTAGGGCAGAAGCAGGACTGGGCCTGTGGNATCTATAGGTAAGATGAATCCCTTTGGGGAAGACCTTGAATAAGAGATTGAAAGGAAGAGTGGGGTCTATCATTTGCAAAG
SEQUENCE_INCLUDED_REGION=34,140
P3_COMMENT=CL is ACATAAACAGCAGCGAAAGG
P3_COMMENT=CR is TGCAAATGATAGACCCCACT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.764198
PRIMER_LEFT_0_PENALTY=0.132262
PRIMER_RIGHT_0_PENALTY=0.631936
PRIMER_INTERNAL_0_PENALTY=0.528861
PRIMER_LEFT_0_SEQUENCE=AACAGCAGCGAAAGGAGAAA
PRIMER_RIGHT_0_SEQUENCE=TCCCCAAAGGGATTCATCTT
PRIMER_INTERNAL_0_SEQUENCE=TTAGGGCAGAAGCAGGACTG
PRIMER_LEFT_0=40,20
PRIMER_RIGHT_0=122,20
PRIMER_INTERNAL_0=63,20
PRIMER_LEFT_0_TM=60.132
PRIMER_RIGHT_0_TM=60.632
PRIMER_INTERNAL_0_TM=60.529
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=83
PRIMER_PAIR_1_PENALTY=0.764198
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.631936
PRIMER_INTERNAL_1_PENALTY=0.528861
PRIMER_LEFT_1_SEQUENCE=AACAGCAGCGAAAGGAGAAA
PRIMER_RIGHT_1_SEQUENCE=TTCCCCAAAGGGATTCATCT
PRIMER_INTERNAL_1_SEQUENCE=TTAGGGCAGAAGCAGGACTG
PRIMER_LEFT_1=40,20
PRIMER_RIGHT_1=123,20
PRIMER_INTERNAL_1=63,20
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.632
PRIMER_INTERNAL_1_TM=60.529
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=0.764198
PRIMER_LEFT_2_PENALTY=0.132262
PRIMER_RIGHT_2_PENALTY=0.631936
PRIMER_INTERNAL_2_PENALTY=0.528861
PRIMER_LEFT_2_SEQUENCE=AACAGCAGCGAAAGGAGAAA
PRIMER_RIGHT_2_SEQUENCE=CTTCCCCAAAGGGATTCATC
PRIMER_INTERNAL_2_SEQUENCE=TTAGGGCAGAAGCAGGACTG
PRIMER_LEFT_2=40,20
PRIMER_RIGHT_2=124,20
PRIMER_INTERNAL_2=63,20
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.632
PRIMER_INTERNAL_2_TM=60.529
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=0.764198
PRIMER_LEFT_3_PENALTY=0.132262
PRIMER_RIGHT_3_PENALTY=0.631936
PRIMER_INTERNAL_3_PENALTY=0.528861
PRIMER_LEFT_3_SEQUENCE=AACAGCAGCGAAAGGAGAAA
PRIMER_RIGHT_3_SEQUENCE=TCTTCCCCAAAGGGATTCAT
PRIMER_INTERNAL_3_SEQUENCE=TTAGGGCAGAAGCAGGACTG
PRIMER_LEFT_3=40,20
PRIMER_RIGHT_3=125,20
PRIMER_INTERNAL_3=63,20
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=60.632
PRIMER_INTERNAL_3_TM=60.529
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=0.764198
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=0.631936
PRIMER_INTERNAL_4_PENALTY=0.528861
PRIMER_LEFT_4_SEQUENCE=AAACAGCAGCGAAAGGAGAA
PRIMER_RIGHT_4_SEQUENCE=TCCCCAAAGGGATTCATCTT
PRIMER_INTERNAL_4_SEQUENCE=TTAGGGCAGAAGCAGGACTG
PRIMER_LEFT_4=39,20
PRIMER_RIGHT_4=122,20
PRIMER_INTERNAL_4=63,20
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=60.632
PRIMER_INTERNAL_4_TM=60.529
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH1020
SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTCCCAAGATCACAGGTCAAGACTTCTACTATCAGGAGACTCATGTCTTTGAATATTTTTCTTCTTTATGCCTCTATGAACAATAGAAATGAAAAGGGGATCTGTTGTATACACTCACAAGGTATACTTTTATTTGTGAAGGATTTTTCAG
SEQUENCE_INCLUDED_REGION=33,154
P3_COMMENT=CL is TTTTCCCAAGATCACAGGTC
P3_COMMENT=CR is CCTTCACAAATAAAAGTATACCTTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.558607
PRIMER_LEFT_0_PENALTY=0.940718
PRIMER_RIGHT_0_PENALTY=3.617890
PRIMER_INTERNAL_0_PENALTY=4.485568
PRIMER_LEFT_0_SEQUENCE=TTCCCAAGATCACAGGTCAA
PRIMER_RIGHT_0_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
PRIMER_INTERNAL_0_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
PRIMER_LEFT_0=36,20
PRIMER_RIGHT_0=134,23
PRIMER_INTERNAL_0=67,22
PRIMER_LEFT_0_TM=59.059
PRIMER_RIGHT_0_TM=59.382
PRIMER_INTERNAL_0_TM=57.514
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=34.783
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=4.558607
PRIMER_LEFT_1_PENALTY=0.940718
PRIMER_RIGHT_1_PENALTY=3.617890
PRIMER_INTERNAL_1_PENALTY=4.485568
PRIMER_LEFT_1_SEQUENCE=TTTCCCAAGATCACAGGTCA
PRIMER_RIGHT_1_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
PRIMER_INTERNAL_1_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
PRIMER_LEFT_1=35,20
PRIMER_RIGHT_1=134,23
PRIMER_INTERNAL_1=67,22
PRIMER_LEFT_1_TM=59.059
PRIMER_RIGHT_1_TM=59.382
PRIMER_INTERNAL_1_TM=57.514
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=4.713782
PRIMER_LEFT_2_PENALTY=1.095892
PRIMER_RIGHT_2_PENALTY=3.617890
PRIMER_INTERNAL_2_PENALTY=4.485568
PRIMER_LEFT_2_SEQUENCE=TTCCCAAGATCACAGGTCAAG
PRIMER_RIGHT_2_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
PRIMER_INTERNAL_2_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
PRIMER_LEFT_2=36,21
PRIMER_RIGHT_2=134,23
PRIMER_INTERNAL_2=67,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.382
PRIMER_INTERNAL_2_TM=57.514
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=4.976841
PRIMER_LEFT_3_PENALTY=1.358951
PRIMER_RIGHT_3_PENALTY=3.617890
PRIMER_INTERNAL_3_PENALTY=4.485568
PRIMER_LEFT_3_SEQUENCE=CCCAAGATCACAGGTCAAGA
PRIMER_RIGHT_3_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
PRIMER_INTERNAL_3_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
PRIMER_LEFT_3=38,20
PRIMER_RIGHT_3=134,23
PRIMER_INTERNAL_3=67,22
PRIMER_LEFT_3_TM=58.641
PRIMER_RIGHT_3_TM=59.382
PRIMER_INTERNAL_3_TM=57.514
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=4.976841
PRIMER_LEFT_4_PENALTY=1.358951
PRIMER_RIGHT_4_PENALTY=3.617890
PRIMER_INTERNAL_4_PENALTY=4.485568
PRIMER_LEFT_4_SEQUENCE=TCCCAAGATCACAGGTCAAG
PRIMER_RIGHT_4_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA
PRIMER_INTERNAL_4_SEQUENCE=TCAGGAGACTCATGTCTTTGAA
PRIMER_LEFT_4=37,20
PRIMER_RIGHT_4=134,23
PRIMER_INTERNAL_4=67,22
PRIMER_LEFT_4_TM=58.641
PRIMER_RIGHT_4_TM=59.382
PRIMER_INTERNAL_4_TM=57.514
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH1022
SEQUENCE_TEMPLATE=GCTTGCATGCTNCAGGTCGACTCTAGAGGATCCCCTTTTTAAAAAAATATTTTCCCATGATTAAACACTTCTAGTGAACTTATAAAGGCCACCACTTCTCTCTCGTTCCTGCTCCTACACCACCACCTNCCCCTCAACTTGTTTTCCTCCTCCTTCACCTCCTGAATCAGCCAGCATAGAGTTTAGTTTATATAAACTTATTCAACCAGGGAAAATTTGGCAGGGATGTCATACTGATTGTTCTCACCTGTGGTAAAGGATTTGTGGATTGAGTCCTCTACTTAGGTACCATTTAATGATTAACCCCAAAGTGGGCAAAGAACCAGAAACCTGGAAG
SEQUENCE_INCLUDED_REGION=35,302
P3_COMMENT=CL is AAAGGCCACCACTTCTCTCT
P3_COMMENT=CR is TCCAGGTTTCTGGTTCTTTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.257880
PRIMER_LEFT_0_PENALTY=0.185942
PRIMER_RIGHT_0_PENALTY=0.071937
PRIMER_INTERNAL_0_PENALTY=5.504493
PRIMER_LEFT_0_SEQUENCE=TTCCTCCTCCTTCACCTCCT
PRIMER_RIGHT_0_SEQUENCE=TCAGTATGACATCCCTGCCA
PRIMER_INTERNAL_0_SEQUENCE=AAACTTATTCAACCAGGGAAAATT
PRIMER_LEFT_0=143,20
PRIMER_RIGHT_0=236,20
PRIMER_INTERNAL_0=193,24
PRIMER_LEFT_0_TM=60.186
PRIMER_RIGHT_0_TM=60.072
PRIMER_INTERNAL_0_TM=58.496
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=1.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=0.268841
PRIMER_LEFT_1_PENALTY=0.196903
PRIMER_RIGHT_1_PENALTY=0.071937
PRIMER_INTERNAL_1_PENALTY=5.504493
PRIMER_LEFT_1_SEQUENCE=CTTCACCTCCTGAATCAGCC
PRIMER_RIGHT_1_SEQUENCE=TCAGTATGACATCCCTGCCA
PRIMER_INTERNAL_1_SEQUENCE=AAACTTATTCAACCAGGGAAAATT
PRIMER_LEFT_1=152,20
PRIMER_RIGHT_1=236,20
PRIMER_INTERNAL_1=193,24
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=60.072
PRIMER_INTERNAL_1_TM=58.496
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=29.167
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=0.268841
PRIMER_LEFT_2_PENALTY=0.196903
PRIMER_RIGHT_2_PENALTY=0.071937
PRIMER_INTERNAL_2_PENALTY=5.504493
PRIMER_LEFT_2_SEQUENCE=CCTTCACCTCCTGAATCAGC
PRIMER_RIGHT_2_SEQUENCE=TCAGTATGACATCCCTGCCA
PRIMER_INTERNAL_2_SEQUENCE=AAACTTATTCAACCAGGGAAAATT
PRIMER_LEFT_2=151,20
PRIMER_RIGHT_2=236,20
PRIMER_INTERNAL_2=193,24
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=60.072
PRIMER_INTERNAL_2_TM=58.496
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=0.413534
PRIMER_LEFT_3_PENALTY=0.185942
PRIMER_RIGHT_3_PENALTY=0.227592
PRIMER_INTERNAL_3_PENALTY=5.552018
PRIMER_LEFT_3_SEQUENCE=TTCCTCCTCCTTCACCTCCT
PRIMER_RIGHT_3_SEQUENCE=CCAAATTTTCCCTGGTTGAA
PRIMER_INTERNAL_3_SEQUENCE=TCAGCCAGCATAGAGTTTAGTTT
PRIMER_LEFT_3=143,20
PRIMER_RIGHT_3=219,20
PRIMER_INTERNAL_3=166,23
PRIMER_LEFT_3_TM=60.186
PRIMER_RIGHT_3_TM=59.772
PRIMER_INTERNAL_3_TM=57.448
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=0.495312
PRIMER_LEFT_4_PENALTY=0.185942
PRIMER_RIGHT_4_PENALTY=0.309369
PRIMER_INTERNAL_4_PENALTY=5.552018
PRIMER_LEFT_4_SEQUENCE=TTCCTCCTCCTTCACCTCCT
PRIMER_RIGHT_4_SEQUENCE=TGACATCCCTGCCAAATTTT
PRIMER_INTERNAL_4_SEQUENCE=TCAGCCAGCATAGAGTTTAGTTT
PRIMER_LEFT_4=143,20
PRIMER_RIGHT_4=230,20
PRIMER_INTERNAL_4=166,23
PRIMER_LEFT_4_TM=60.186
PRIMER_RIGHT_4_TM=60.309
PRIMER_INTERNAL_4_TM=57.448
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH1026
SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTAAGCACTGAATGAAAATTAATTGTTAAATTCTGAGAATTGAACCTTCTTGTATTTTAATTTTGTTCAAACGTATTTTTTTGTTTTTCTTTTTTTTGTAAAAAAAAGAAAAAGCACAAACTTTCTTCTGCCTGCAACATTTCAGTAGGGTNNAACATCCTTTCTTTGTATTTGAATTCACTTTTTTGATGCAAGAAGAAAGGGAAAGATCTTGATGCAGGAAAATGGCATTGGCTCACAGATGCTATCTGCCATGCTTTTGTGCAG
SEQUENCE_INCLUDED_REGION=34,267
P3_COMMENT=CL is ATTCTGAGAATTGAACCTTCTTG
P3_COMMENT=CR is ACAAAAGCATGGCAGATAGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.383098
PRIMER_LEFT_0_PENALTY=0.012060
PRIMER_RIGHT_0_PENALTY=0.371037
PRIMER_INTERNAL_0_PENALTY=3.367771
PRIMER_LEFT_0_SEQUENCE=TTCTGCCTGCAACATTTCAG
PRIMER_RIGHT_0_SEQUENCE=CCTGCATCAAGATCTTTCCC
PRIMER_INTERNAL_0_SEQUENCE=TTGAATTCACTTTTTTGATGCAA
PRIMER_LEFT_0=159,20
PRIMER_RIGHT_0=254,20
PRIMER_INTERNAL_0=205,23
PRIMER_LEFT_0_TM=59.988
PRIMER_RIGHT_0_TM=59.629
PRIMER_INTERNAL_0_TM=59.632
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=26.087
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=0.383098
PRIMER_LEFT_1_PENALTY=0.012060
PRIMER_RIGHT_1_PENALTY=0.371037
PRIMER_INTERNAL_1_PENALTY=3.367771
PRIMER_LEFT_1_SEQUENCE=CTTCTGCCTGCAACATTTCA
PRIMER_RIGHT_1_SEQUENCE=CCTGCATCAAGATCTTTCCC
PRIMER_INTERNAL_1_SEQUENCE=TTGAATTCACTTTTTTGATGCAA
PRIMER_LEFT_1=158,20
PRIMER_RIGHT_1=254,20
PRIMER_INTERNAL_1=205,23
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=59.629
PRIMER_INTERNAL_1_TM=59.632
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=26.087
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=0.925873
PRIMER_LEFT_2_PENALTY=0.554836
PRIMER_RIGHT_2_PENALTY=0.371037
PRIMER_INTERNAL_2_PENALTY=3.367771
PRIMER_LEFT_2_SEQUENCE=TCTGCCTGCAACATTTCAGT
PRIMER_RIGHT_2_SEQUENCE=CCTGCATCAAGATCTTTCCC
PRIMER_INTERNAL_2_SEQUENCE=TTGAATTCACTTTTTTGATGCAA
PRIMER_LEFT_2=160,20
PRIMER_RIGHT_2=254,20
PRIMER_INTERNAL_2=205,23
PRIMER_LEFT_2_TM=59.445
PRIMER_RIGHT_2_TM=59.629
PRIMER_INTERNAL_2_TM=59.632
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=26.087
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=1.203231
PRIMER_LEFT_3_PENALTY=0.012060
PRIMER_RIGHT_3_PENALTY=1.191170
PRIMER_INTERNAL_3_PENALTY=6.335368
PRIMER_LEFT_3_SEQUENCE=TTCTGCCTGCAACATTTCAG
PRIMER_RIGHT_3_SEQUENCE=TCCCTTTCTTCTTGCATCAAA
PRIMER_INTERNAL_3_SEQUENCE=CATCCTTTCTTTGTATTTGAATTCA
PRIMER_LEFT_3=159,20
PRIMER_RIGHT_3=238,21
PRIMER_INTERNAL_3=189,25
PRIMER_LEFT_3_TM=59.988
PRIMER_RIGHT_3_TM=59.809
PRIMER_INTERNAL_3_TM=58.665
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_INTERNAL_3_GC_PERCENT=28.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=1.203231
PRIMER_LEFT_4_PENALTY=0.012060
PRIMER_RIGHT_4_PENALTY=1.191170
PRIMER_INTERNAL_4_PENALTY=6.335368
PRIMER_LEFT_4_SEQUENCE=TTCTGCCTGCAACATTTCAG
PRIMER_RIGHT_4_SEQUENCE=TTCCCTTTCTTCTTGCATCAA
PRIMER_INTERNAL_4_SEQUENCE=CATCCTTTCTTTGTATTTGAATTCA
PRIMER_LEFT_4=159,20
PRIMER_RIGHT_4=239,21
PRIMER_INTERNAL_4=189,25
PRIMER_LEFT_4_TM=59.988
PRIMER_RIGHT_4_TM=59.809
PRIMER_INTERNAL_4_TM=58.665
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=28.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH1027
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTCTTATTCCATTCTGCCTCTCTCAGGGTGTTCTCCTCTGCCAAAGTGGGGGGACCCTCTTCTGATGTTTACGCGAGTCTCAAGTCCCCAGGGATTCATCTCACTGTGATTTAGCCAGGGCCTTGTTGCTTCTTCGGGAATCAGATGGCTGGACCACGAAAGCCCAGCGCCTTTCCTATCCTGAAAGAAG
SEQUENCE_INCLUDED_REGION=36,192
P3_COMMENT=CL is GGGACCCTCTTCTGATGTTT
P3_COMMENT=CR is TTCTTTCAGGATAGGAAAGGC
P3_COMMENT=CM is CGCGAGTCTCAAGTCCCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.085332
PRIMER_LEFT_0_PENALTY=0.014419
PRIMER_RIGHT_0_PENALTY=0.070913
PRIMER_INTERNAL_0_PENALTY=0.314094
PRIMER_LEFT_0_SEQUENCE=TGCCTCTCTCAGGGTGTTCT
PRIMER_RIGHT_0_SEQUENCE=ATCCCTGGGGACTTGAGACT
PRIMER_INTERNAL_0_SEQUENCE=GGGGACCCTCTTCTGATGTT
PRIMER_LEFT_0=52,20
PRIMER_RIGHT_0=132,20
PRIMER_INTERNAL_0=87,20
PRIMER_LEFT_0_TM=59.986
PRIMER_RIGHT_0_TM=59.929
PRIMER_INTERNAL_0_TM=60.314
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=0.085332
PRIMER_LEFT_1_PENALTY=0.014419
PRIMER_RIGHT_1_PENALTY=0.070913
PRIMER_INTERNAL_1_PENALTY=0.314094
PRIMER_LEFT_1_SEQUENCE=CTGCCTCTCTCAGGGTGTTC
PRIMER_RIGHT_1_SEQUENCE=ATCCCTGGGGACTTGAGACT
PRIMER_INTERNAL_1_SEQUENCE=GGGGACCCTCTTCTGATGTT
PRIMER_LEFT_1=51,20
PRIMER_RIGHT_1=132,20
PRIMER_INTERNAL_1=87,20
PRIMER_LEFT_1_TM=59.986
PRIMER_RIGHT_1_TM=59.929
PRIMER_INTERNAL_1_TM=60.314
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.085332
PRIMER_LEFT_2_PENALTY=0.014419
PRIMER_RIGHT_2_PENALTY=0.070913
PRIMER_INTERNAL_2_PENALTY=0.314094
PRIMER_LEFT_2_SEQUENCE=TCTGCCTCTCTCAGGGTGTT
PRIMER_RIGHT_2_SEQUENCE=ATCCCTGGGGACTTGAGACT
PRIMER_INTERNAL_2_SEQUENCE=GGGGACCCTCTTCTGATGTT
PRIMER_LEFT_2=50,20
PRIMER_RIGHT_2=132,20
PRIMER_INTERNAL_2=87,20
PRIMER_LEFT_2_TM=59.986
PRIMER_RIGHT_2_TM=59.929
PRIMER_INTERNAL_2_TM=60.314
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.085332
PRIMER_LEFT_3_PENALTY=0.014419
PRIMER_RIGHT_3_PENALTY=0.070913
PRIMER_INTERNAL_3_PENALTY=0.314094
PRIMER_LEFT_3_SEQUENCE=TTCTGCCTCTCTCAGGGTGT
PRIMER_RIGHT_3_SEQUENCE=ATCCCTGGGGACTTGAGACT
PRIMER_INTERNAL_3_SEQUENCE=GGGGACCCTCTTCTGATGTT
PRIMER_LEFT_3=49,20
PRIMER_RIGHT_3=132,20
PRIMER_INTERNAL_3=87,20
PRIMER_LEFT_3_TM=59.986
PRIMER_RIGHT_3_TM=59.929
PRIMER_INTERNAL_3_TM=60.314
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=0.259542
PRIMER_LEFT_4_PENALTY=0.014419
PRIMER_RIGHT_4_PENALTY=0.245123
PRIMER_INTERNAL_4_PENALTY=0.314094
PRIMER_LEFT_4_SEQUENCE=CTCAGGGTGTTCTCCTCTGC
PRIMER_RIGHT_4_SEQUENCE=AGATGAATCCCTGGGGACTT
PRIMER_INTERNAL_4_SEQUENCE=GGGGACCCTCTTCTGATGTT
PRIMER_LEFT_4=59,20
PRIMER_RIGHT_4=138,20
PRIMER_INTERNAL_4=87,20
PRIMER_LEFT_4_TM=59.986
PRIMER_RIGHT_4_TM=59.755
PRIMER_INTERNAL_4_TM=60.314
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH1033
SEQUENCE_TEMPLATE=ANTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGCCAAAGCCACACCTGAACCCCTTTAAACCATACCTGGGGTGGTCAAGGAGTCTGCATCAGAATGTGGGAAGCAGAGACTTGAGGCAGCCCTGGGAAGTAAACCCCAAGATCTTCAGCGCACCCTAGGCTCTCTTTTGCAGGATTTCCTTCCCTCAGATCATGACACTCTGGGCCTATGACGGGAGGNGCTACGCAGTAATCTNCAAAGTACCTTTGAGGTTATTCTCCCATTGTCCTAATTAGTAG
SEQUENCE_INCLUDED_REGION=33,252
P3_COMMENT=CL is CCTGAACCCCTTTAAACCAT
P3_COMMENT=CR is ATGGGAGAATAACCTCAAAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.142150
PRIMER_LEFT_0_PENALTY=0.119809
PRIMER_RIGHT_0_PENALTY=0.022341
PRIMER_INTERNAL_0_PENALTY=0.102380
PRIMER_LEFT_0_SEQUENCE=GGTGGTCAAGGAGTCTGCAT
PRIMER_RIGHT_0_SEQUENCE=TAGGGTGCGCTGAAGATCTT
PRIMER_INTERNAL_0_SEQUENCE=AAGCAGAGACTTGAGGCAGC
PRIMER_LEFT_0=76,20
PRIMER_RIGHT_0=163,20
PRIMER_INTERNAL_0=107,20
PRIMER_LEFT_0_TM=60.120
PRIMER_RIGHT_0_TM=59.978
PRIMER_INTERNAL_0_TM=59.898
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=8.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.223326
PRIMER_LEFT_1_PENALTY=0.200985
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_INTERNAL_1_PENALTY=1.312895
PRIMER_LEFT_1_SEQUENCE=TCTGCATCAGAATGTGGGAA
PRIMER_RIGHT_1_SEQUENCE=TAGGGTGCGCTGAAGATCTT
PRIMER_INTERNAL_1_SEQUENCE=AGCCCTGGGAAGTAAACCC
PRIMER_LEFT_1=89,20
PRIMER_RIGHT_1=163,20
PRIMER_INTERNAL_1=124,19
PRIMER_LEFT_1_TM=60.201
PRIMER_RIGHT_1_TM=59.978
PRIMER_INTERNAL_1_TM=60.313
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=8.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=0.278771
PRIMER_LEFT_2_PENALTY=0.176390
PRIMER_RIGHT_2_PENALTY=0.102380
PRIMER_INTERNAL_2_PENALTY=0.119809
PRIMER_LEFT_2_SEQUENCE=CCACACCTGAACCCCTTTAA
PRIMER_RIGHT_2_SEQUENCE=GCTGCCTCAAGTCTCTGCTT
PRIMER_INTERNAL_2_SEQUENCE=GGTGGTCAAGGAGTCTGCAT
PRIMER_LEFT_2=45,20
PRIMER_RIGHT_2=126,20
PRIMER_INTERNAL_2=76,20
PRIMER_LEFT_2_TM=59.824
PRIMER_RIGHT_2_TM=59.898
PRIMER_INTERNAL_2_TM=60.120
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=0.385340
PRIMER_LEFT_3_PENALTY=0.362999
PRIMER_RIGHT_3_PENALTY=0.022341
PRIMER_INTERNAL_3_PENALTY=1.312895
PRIMER_LEFT_3_SEQUENCE=GTCTGCATCAGAATGTGGGA
PRIMER_RIGHT_3_SEQUENCE=TAGGGTGCGCTGAAGATCTT
PRIMER_INTERNAL_3_SEQUENCE=AGCCCTGGGAAGTAAACCC
PRIMER_LEFT_3=88,20
PRIMER_RIGHT_3=163,20
PRIMER_INTERNAL_3=124,19
PRIMER_LEFT_3_TM=59.637
PRIMER_RIGHT_3_TM=59.978
PRIMER_INTERNAL_3_TM=60.313
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=8.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=0.424452
PRIMER_LEFT_4_PENALTY=0.402111
PRIMER_RIGHT_4_PENALTY=0.022341
PRIMER_INTERNAL_4_PENALTY=0.102380
PRIMER_LEFT_4_SEQUENCE=TGGTCAAGGAGTCTGCATCA
PRIMER_RIGHT_4_SEQUENCE=TAGGGTGCGCTGAAGATCTT
PRIMER_INTERNAL_4_SEQUENCE=AAGCAGAGACTTGAGGCAGC
PRIMER_LEFT_4=78,20
PRIMER_RIGHT_4=163,20
PRIMER_INTERNAL_4=107,20
PRIMER_LEFT_4_TM=60.402
PRIMER_RIGHT_4_TM=59.978
PRIMER_INTERNAL_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=8.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH1034
SEQUENCE_TEMPLATE=GCATGNCTNCAGGTCGACTCTAGAGGATCCCCTTCCTGGTTGGCAAGACTTCATAAATTTTGCCATTATTTGAAGATAACCCACACCTTGGTGTCCAGGGTTTTCACAGGTATTAGTGGTCAGTCACATAGGCATATAGTACCTGTATGACTTCTATTCCAGCCACCGCAAACTTCTCCTCTCCCTGCTGGCTCCTGAGCCAAAAACAGGCATTTACCATAAATCACTTTGTTAGGATGAACTTATCTGGCCAAACTGATACGGCATGACCCACAGCCTCAGGTATATAAAACACTCTCATCAGGCAGAATGTTTCAGGGTCTCAGAGGTTAGTCAGGGGCTAGTCAAGGACCAATCCTGAAGGCAGGCC
SEQUENCE_INCLUDED_REGION=32,338
P3_COMMENT=CL is TTTGCCATTATTTGAAGATAACC
P3_COMMENT=CR is GGATTGGTCCTTGACTAGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.155909
PRIMER_LEFT_0_PENALTY=0.044832
PRIMER_RIGHT_0_PENALTY=0.111077
PRIMER_INTERNAL_0_PENALTY=0.532607
PRIMER_LEFT_0_SEQUENCE=ATCTGGCCAAACTGATACGG
PRIMER_RIGHT_0_SEQUENCE=ACCCTGAAACATTCTGCCTG
PRIMER_INTERNAL_0_SEQUENCE=TGACCCACAGCCTCAGGTAT
PRIMER_LEFT_0=244,20
PRIMER_RIGHT_0=320,20
PRIMER_INTERNAL_0=266,20
PRIMER_LEFT_0_TM=59.955
PRIMER_RIGHT_0_TM=60.111
PRIMER_INTERNAL_0_TM=60.533
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=0.409870
PRIMER_LEFT_1_PENALTY=0.399325
PRIMER_RIGHT_1_PENALTY=0.010545
PRIMER_INTERNAL_1_PENALTY=3.690231
PRIMER_LEFT_1_SEQUENCE=TGTCCAGGGTTTTCACAGGT
PRIMER_RIGHT_1_SEQUENCE=GAGAGGAGAAGTTTGCGGTG
PRIMER_INTERNAL_1_SEQUENCE=TTAGTGGTCAGTCACATAGGCA
PRIMER_LEFT_1=91,20
PRIMER_RIGHT_1=182,20
PRIMER_INTERNAL_1=112,22
PRIMER_LEFT_1_TM=60.399
PRIMER_RIGHT_1_TM=59.989
PRIMER_INTERNAL_1_TM=58.310
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=92
PRIMER_PAIR_2_PENALTY=0.409870
PRIMER_LEFT_2_PENALTY=0.399325
PRIMER_RIGHT_2_PENALTY=0.010545
PRIMER_INTERNAL_2_PENALTY=3.690231
PRIMER_LEFT_2_SEQUENCE=GTGTCCAGGGTTTTCACAGG
PRIMER_RIGHT_2_SEQUENCE=GAGAGGAGAAGTTTGCGGTG
PRIMER_INTERNAL_2_SEQUENCE=TTAGTGGTCAGTCACATAGGCA
PRIMER_LEFT_2=90,20
PRIMER_RIGHT_2=182,20
PRIMER_INTERNAL_2=112,22
PRIMER_LEFT_2_TM=60.399
PRIMER_RIGHT_2_TM=59.989
PRIMER_INTERNAL_2_TM=58.310
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=93
PRIMER_PAIR_3_PENALTY=0.409870
PRIMER_LEFT_3_PENALTY=0.399325
PRIMER_RIGHT_3_PENALTY=0.010545
PRIMER_INTERNAL_3_PENALTY=3.690231
PRIMER_LEFT_3_SEQUENCE=GGTGTCCAGGGTTTTCACAG
PRIMER_RIGHT_3_SEQUENCE=GAGAGGAGAAGTTTGCGGTG
PRIMER_INTERNAL_3_SEQUENCE=TTAGTGGTCAGTCACATAGGCA
PRIMER_LEFT_3=89,20
PRIMER_RIGHT_3=182,20
PRIMER_INTERNAL_3=112,22
PRIMER_LEFT_3_TM=60.399
PRIMER_RIGHT_3_TM=59.989
PRIMER_INTERNAL_3_TM=58.310
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=0.429134
PRIMER_LEFT_4_PENALTY=0.248900
PRIMER_RIGHT_4_PENALTY=0.180233
PRIMER_INTERNAL_4_PENALTY=3.485357
PRIMER_LEFT_4_SEQUENCE=CTGAGCCAAAAACAGGCATT
PRIMER_RIGHT_4_SEQUENCE=GGGTCATGCCGTATCAGTTT
PRIMER_INTERNAL_4_SEQUENCE=TGTTAGGATGAACTTATCTGGCC
PRIMER_LEFT_4=194,20
PRIMER_RIGHT_4=271,20
PRIMER_INTERNAL_4=229,23
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.820
PRIMER_INTERNAL_4_TM=59.515
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH1036
SEQUENCE_TEMPLATE=AGACAGGAGGAAGGCAGCAGAATGGTCTGACAAAGATGTCTGCAGGCAGGAGGGTCTGCAGAATTCATACCTAATTCCCTCAGTTTTCTTGGTGAACCAGGGGGTGGTGACTTGTGCTAGGCACCCAAACTAGAGACCAATCACTCCTTTTCACAAACATTTTATAAAGAATAGTCTTGTTCTCTTCTAATGAAGAAAGAGTTTTCTCTGCTCTTTTAGTTAGGCTGCTGAGCAC
SEQUENCE_INCLUDED_REGION=0,235
P3_COMMENT=CL is CAGCAGAATGGTCTGACAAA
P3_COMMENT=CR is TGCTCAGCAGCCTAACTAAA
P3_COMMENT=CM is ATGTCTGCAGGCAGGAGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.103949
PRIMER_LEFT_0_PENALTY=0.008461
PRIMER_RIGHT_0_PENALTY=0.095488
PRIMER_INTERNAL_0_PENALTY=0.575355
PRIMER_LEFT_0_SEQUENCE=GACAAAGATGTCTGCAGGCA
PRIMER_RIGHT_0_SEQUENCE=TGCCTAGCACAAGTCACCAC
PRIMER_INTERNAL_0_SEQUENCE=GTTTTCTTGGTGAACCAGGG
PRIMER_LEFT_0=28,20
PRIMER_RIGHT_0=122,20
PRIMER_INTERNAL_0=82,20
PRIMER_LEFT_0_TM=59.992
PRIMER_RIGHT_0_TM=59.905
PRIMER_INTERNAL_0_TM=59.425
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=0.103949
PRIMER_LEFT_1_PENALTY=0.008461
PRIMER_RIGHT_1_PENALTY=0.095488
PRIMER_INTERNAL_1_PENALTY=0.575355
PRIMER_LEFT_1_SEQUENCE=GACAAAGATGTCTGCAGGCA
PRIMER_RIGHT_1_SEQUENCE=GTGCCTAGCACAAGTCACCA
PRIMER_INTERNAL_1_SEQUENCE=GTTTTCTTGGTGAACCAGGG
PRIMER_LEFT_1=28,20
PRIMER_RIGHT_1=123,20
PRIMER_INTERNAL_1=82,20
PRIMER_LEFT_1_TM=59.992
PRIMER_RIGHT_1_TM=59.905
PRIMER_INTERNAL_1_TM=59.425
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=8.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=0.103949
PRIMER_LEFT_2_PENALTY=0.008461
PRIMER_RIGHT_2_PENALTY=0.095488
PRIMER_INTERNAL_2_PENALTY=0.196903
PRIMER_LEFT_2_SEQUENCE=TGACAAAGATGTCTGCAGGC
PRIMER_RIGHT_2_SEQUENCE=TGCCTAGCACAAGTCACCAC
PRIMER_INTERNAL_2_SEQUENCE=AGGAGGGTCTGCAGAATTCA
PRIMER_LEFT_2=27,20
PRIMER_RIGHT_2=122,20
PRIMER_INTERNAL_2=47,20
PRIMER_LEFT_2_TM=59.992
PRIMER_RIGHT_2_TM=59.905
PRIMER_INTERNAL_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.103949
PRIMER_LEFT_3_PENALTY=0.008461
PRIMER_RIGHT_3_PENALTY=0.095488
PRIMER_INTERNAL_3_PENALTY=0.196903
PRIMER_LEFT_3_SEQUENCE=TGACAAAGATGTCTGCAGGC
PRIMER_RIGHT_3_SEQUENCE=GTGCCTAGCACAAGTCACCA
PRIMER_INTERNAL_3_SEQUENCE=AGGAGGGTCTGCAGAATTCA
PRIMER_LEFT_3=27,20
PRIMER_RIGHT_3=123,20
PRIMER_INTERNAL_3=47,20
PRIMER_LEFT_3_TM=59.992
PRIMER_RIGHT_3_TM=59.905
PRIMER_INTERNAL_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=0.292391
PRIMER_LEFT_4_PENALTY=0.196903
PRIMER_RIGHT_4_PENALTY=0.095488
PRIMER_INTERNAL_4_PENALTY=0.575355
PRIMER_LEFT_4_SEQUENCE=AGGAGGGTCTGCAGAATTCA
PRIMER_RIGHT_4_SEQUENCE=TGCCTAGCACAAGTCACCAC
PRIMER_INTERNAL_4_SEQUENCE=GTTTTCTTGGTGAACCAGGG
PRIMER_LEFT_4=47,20
PRIMER_RIGHT_4=122,20
PRIMER_INTERNAL_4=82,20
PRIMER_LEFT_4_TM=59.803
PRIMER_RIGHT_4_TM=59.905
PRIMER_INTERNAL_4_TM=59.425
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1040
SEQUENCE_TEMPLATE=CNNCNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACAAAAAGATAAGCAAAGAAGGAATGATATTAAAACTCTTTTACCTGGAGAAGAGGGTTGCATTCCNTTAGACTGTATAATGTGGCTTCAGACAGTGATGCAGGGGGAAGTTATATGCCTTCCTGGGAACTCATGATTAGAGAAGTCAGTAGAAGAAAACAAAGTTGAGAGTGAGGCCAAGACACGACCAGAGAAAAATGAGAGAGTTGTTCATTGAATGAGTTAAGTCAAAGAAAATGGAAGGAAAATTATGAGGTCTGTGGGCTATCTAATGCTCCTATTATGAAGTGTGGTTGAGTTGGAGG
SEQUENCE_INCLUDED_REGION=33,309
P3_COMMENT=CL is CACAAAAAGATAAGCAAAGAAGG
P3_COMMENT=CR is TCCAACTCAACCACACTTCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.531943
PRIMER_LEFT_0_PENALTY=0.077340
PRIMER_RIGHT_0_PENALTY=0.454603
PRIMER_INTERNAL_0_PENALTY=3.081536
PRIMER_LEFT_0_SEQUENCE=TGCAGGGGGAAGTTATATGC
PRIMER_RIGHT_0_SEQUENCE=TGTCTTGGCCTCACTCTCAA
PRIMER_INTERNAL_0_SEQUENCE=TCCTGGGAACTCATGATTAGAGA
PRIMER_LEFT_0=135,20
PRIMER_RIGHT_0=220,20
PRIMER_INTERNAL_0=157,23
PRIMER_LEFT_0_TM=59.923
PRIMER_RIGHT_0_TM=59.545
PRIMER_INTERNAL_0_TM=60.082
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=0.788943
PRIMER_LEFT_1_PENALTY=0.195358
PRIMER_RIGHT_1_PENALTY=0.593585
PRIMER_INTERNAL_1_PENALTY=0.422979
PRIMER_LEFT_1_SEQUENCE=TGGAGAAGAGGGTTGCATTC
PRIMER_RIGHT_1_SEQUENCE=TGAGTTCCCAGGAAGGCATA
PRIMER_INTERNAL_1_SEQUENCE=GCTTCAGACAGTGATGCAGG
PRIMER_LEFT_1=82,20
PRIMER_RIGHT_1=169,20
PRIMER_INTERNAL_1=121,20
PRIMER_LEFT_1_TM=60.195
PRIMER_RIGHT_1_TM=60.594
PRIMER_INTERNAL_1_TM=59.577
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=0.793466
PRIMER_LEFT_2_PENALTY=0.716126
PRIMER_RIGHT_2_PENALTY=0.077340
PRIMER_INTERNAL_2_PENALTY=4.642980
PRIMER_LEFT_2_SEQUENCE=TACCTGGAGAAGAGGGTTGC
PRIMER_RIGHT_2_SEQUENCE=GCATATAACTTCCCCCTGCA
PRIMER_INTERNAL_2_SEQUENCE=ATAATGTGGCTTCAGACAGTGA
PRIMER_LEFT_2=78,20
PRIMER_RIGHT_2=154,20
PRIMER_INTERNAL_2=113,22
PRIMER_LEFT_2_TM=59.284
PRIMER_RIGHT_2_TM=59.923
PRIMER_INTERNAL_2_TM=57.357
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=0.877581
PRIMER_LEFT_3_PENALTY=0.422979
PRIMER_RIGHT_3_PENALTY=0.454603
PRIMER_INTERNAL_3_PENALTY=0.593585
PRIMER_LEFT_3_SEQUENCE=GCTTCAGACAGTGATGCAGG
PRIMER_RIGHT_3_SEQUENCE=TGTCTTGGCCTCACTCTCAA
PRIMER_INTERNAL_3_SEQUENCE=TATGCCTTCCTGGGAACTCA
PRIMER_LEFT_3=121,20
PRIMER_RIGHT_3=220,20
PRIMER_INTERNAL_3=150,20
PRIMER_LEFT_3_TM=59.577
PRIMER_RIGHT_3_TM=59.545
PRIMER_INTERNAL_3_TM=60.594
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=0.904432
PRIMER_LEFT_4_PENALTY=0.449829
PRIMER_RIGHT_4_PENALTY=0.454603
PRIMER_INTERNAL_4_PENALTY=0.593585
PRIMER_LEFT_4_SEQUENCE=AGTGATGCAGGGGGAAGTTA
PRIMER_RIGHT_4_SEQUENCE=TGTCTTGGCCTCACTCTCAA
PRIMER_INTERNAL_4_SEQUENCE=TATGCCTTCCTGGGAACTCA
PRIMER_LEFT_4=130,20
PRIMER_RIGHT_4=220,20
PRIMER_INTERNAL_4=150,20
PRIMER_LEFT_4_TM=59.550
PRIMER_RIGHT_4_TM=59.545
PRIMER_INTERNAL_4_TM=60.594
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH1041
SEQUENCE_TEMPLATE=GCTNCNNGCCTCCNGGTCGACTCTAGAGGATCCCTGTCCCAAATCAATCTTACCAGAACAATCTCAAAANANATGTTTTCTCTTCTGATATACATGAACATGGCCCAAAAAACATAAAAAAAAGAAGAAAATACCTCTTACTGGTTGATGAGGACCCCACATACATCCTGAGAATAAGGCACACGTAATGGATTGTGAAACCTCACATGAGAAATAGGTCTGGATGATGTTGTTTGGGCTTCCAAATCAAACAGTAG
SEQUENCE_INCLUDED_REGION=34,223
P3_COMMENT=CL is CCAAATCAATCTTACCAGAACA
P3_COMMENT=CR is GAAGCCCAAACAACATCATC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.236882
PRIMER_LEFT_0_PENALTY=0.152999
PRIMER_RIGHT_0_PENALTY=0.083883
PRIMER_INTERNAL_0_PENALTY=3.004943
PRIMER_LEFT_0_SEQUENCE=GGCACACGTAATGGATTGTG
PRIMER_RIGHT_0_SEQUENCE=GTTTGATTTGGAAGCCCAAA
PRIMER_INTERNAL_0_SEQUENCE=CCTCACATGAGAAATAGGTCTGG
PRIMER_LEFT_0=177,20
PRIMER_RIGHT_0=251,20
PRIMER_INTERNAL_0=200,23
PRIMER_LEFT_0_TM=59.847
PRIMER_RIGHT_0_TM=59.916
PRIMER_INTERNAL_0_TM=60.005
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.441344
PRIMER_LEFT_1_PENALTY=0.237978
PRIMER_RIGHT_1_PENALTY=0.203367
PRIMER_INTERNAL_1_PENALTY=0.152999
PRIMER_LEFT_1_SEQUENCE=TGATGAGGACCCCACATACA
PRIMER_RIGHT_1_SEQUENCE=AAGCCCAAACAACATCATCC
PRIMER_INTERNAL_1_SEQUENCE=GGCACACGTAATGGATTGTG
PRIMER_LEFT_1=145,20
PRIMER_RIGHT_1=240,20
PRIMER_INTERNAL_1=177,20
PRIMER_LEFT_1_TM=59.762
PRIMER_RIGHT_1_TM=59.797
PRIMER_INTERNAL_1_TM=59.847
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=0.441344
PRIMER_LEFT_2_PENALTY=0.237978
PRIMER_RIGHT_2_PENALTY=0.203367
PRIMER_INTERNAL_2_PENALTY=0.152999
PRIMER_LEFT_2_SEQUENCE=TGATGAGGACCCCACATACA
PRIMER_RIGHT_2_SEQUENCE=GGAAGCCCAAACAACATCAT
PRIMER_INTERNAL_2_SEQUENCE=GGCACACGTAATGGATTGTG
PRIMER_LEFT_2=145,20
PRIMER_RIGHT_2=242,20
PRIMER_INTERNAL_2=177,20
PRIMER_LEFT_2_TM=59.762
PRIMER_RIGHT_2_TM=59.797
PRIMER_INTERNAL_2_TM=59.847
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=98
PRIMER_PAIR_3_PENALTY=0.452013
PRIMER_LEFT_3_PENALTY=0.214036
PRIMER_RIGHT_3_PENALTY=0.237978
PRIMER_INTERNAL_3_PENALTY=8.781094
PRIMER_LEFT_3_SEQUENCE=TACATGAACATGGCCCAAAA
PRIMER_RIGHT_3_SEQUENCE=TGTATGTGGGGTCCTCATCA
PRIMER_INTERNAL_3_SEQUENCE=AAAAAAGAAGAAAATACCTCTTACTGG
PRIMER_LEFT_3=90,20
PRIMER_RIGHT_3=164,20
PRIMER_INTERNAL_3=117,27
PRIMER_LEFT_3_TM=59.786
PRIMER_RIGHT_3_TM=59.762
PRIMER_INTERNAL_3_TM=58.219
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=29.630
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=0.548580
PRIMER_LEFT_4_PENALTY=0.345214
PRIMER_RIGHT_4_PENALTY=0.203367
PRIMER_INTERNAL_4_PENALTY=0.152999
PRIMER_LEFT_4_SEQUENCE=AGGACCCCACATACATCCTG
PRIMER_RIGHT_4_SEQUENCE=AAGCCCAAACAACATCATCC
PRIMER_INTERNAL_4_SEQUENCE=GGCACACGTAATGGATTGTG
PRIMER_LEFT_4=150,20
PRIMER_RIGHT_4=240,20
PRIMER_INTERNAL_4=177,20
PRIMER_LEFT_4_TM=59.655
PRIMER_RIGHT_4_TM=59.797
PRIMER_INTERNAL_4_TM=59.847
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH1044
SEQUENCE_TEMPLATE=NNTNCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTAGCCTGCTTCATGGCAAGGAAAACAAAGTTATCTCAGCCTCATTTAGTACATTTCCTGCCCCACTCCTNNNTAAGAATCAATTTCTCCAATGAGCCCTGGTTCCTTTTACTAGGAAATGGTATTTAGAAACCACAGTCTGGGCTTTAGGGGTAGGCATTGGCCTTTTTAGTAACAGAACTAGTATTTTGGTATTTNAGGTAAATAAAACATATTTCAAATTCAAATTTAAAA
SEQUENCE_INCLUDED_REGION=35,234
P3_COMMENT=CL is CATGGCAAGGAAAACAAAGT
P3_COMMENT=CR is GCCCAGACTGTGGTTTCTAA
P3_COMMENT=CM is ATCTCAGCCTCATTTAGTACATTTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.019181
PRIMER_LEFT_0_PENALTY=0.013607
PRIMER_RIGHT_0_PENALTY=0.005574
PRIMER_INTERNAL_0_PENALTY=4.640064
PRIMER_LEFT_0_SEQUENCE=TCAATTTCTCCAATGAGCCC
PRIMER_RIGHT_0_SEQUENCE=ACCCCTAAAGCCCAGACTGT
PRIMER_INTERNAL_0_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
PRIMER_LEFT_0=114,20
PRIMER_RIGHT_0=188,20
PRIMER_INTERNAL_0=134,24
PRIMER_LEFT_0_TM=60.014
PRIMER_RIGHT_0_TM=59.994
PRIMER_INTERNAL_0_TM=59.360
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.058511
PRIMER_LEFT_1_PENALTY=0.013607
PRIMER_RIGHT_1_PENALTY=0.044904
PRIMER_INTERNAL_1_PENALTY=4.640064
PRIMER_LEFT_1_SEQUENCE=TCAATTTCTCCAATGAGCCC
PRIMER_RIGHT_1_SEQUENCE=CCTACCCCTAAAGCCCAGAC
PRIMER_INTERNAL_1_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
PRIMER_LEFT_1=114,20
PRIMER_RIGHT_1=191,20
PRIMER_INTERNAL_1=134,24
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=59.955
PRIMER_INTERNAL_1_TM=59.360
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=0.822899
PRIMER_LEFT_2_PENALTY=0.013607
PRIMER_RIGHT_2_PENALTY=0.809292
PRIMER_INTERNAL_2_PENALTY=4.640064
PRIMER_LEFT_2_SEQUENCE=TCAATTTCTCCAATGAGCCC
PRIMER_RIGHT_2_SEQUENCE=TACCCCTAAAGCCCAGACTG
PRIMER_INTERNAL_2_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
PRIMER_LEFT_2=114,20
PRIMER_RIGHT_2=189,20
PRIMER_INTERNAL_2=134,24
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=59.191
PRIMER_INTERNAL_2_TM=59.360
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=10.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=8.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=0.825095
PRIMER_LEFT_3_PENALTY=0.013607
PRIMER_RIGHT_3_PENALTY=0.811488
PRIMER_INTERNAL_3_PENALTY=4.640064
PRIMER_LEFT_3_SEQUENCE=TCAATTTCTCCAATGAGCCC
PRIMER_RIGHT_3_SEQUENCE=CAATGCCTACCCCTAAAGCC
PRIMER_INTERNAL_3_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT
PRIMER_LEFT_3=114,20
PRIMER_RIGHT_3=196,20
PRIMER_INTERNAL_3=134,24
PRIMER_LEFT_3_TM=60.014
PRIMER_RIGHT_3_TM=60.811
PRIMER_INTERNAL_3_TM=59.360
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=0.825095
PRIMER_LEFT_4_PENALTY=0.013607
PRIMER_RIGHT_4_PENALTY=0.811488
PRIMER_INTERNAL_4_PENALTY=4.529250
PRIMER_LEFT_4_SEQUENCE=TCAATTTCTCCAATGAGCCC
PRIMER_RIGHT_4_SEQUENCE=CCAATGCCTACCCCTAAAGC
PRIMER_INTERNAL_4_SEQUENCE=TGGTATTTAGAAACCACAGTCTGG
PRIMER_LEFT_4=114,20
PRIMER_RIGHT_4=197,20
PRIMER_INTERNAL_4=154,24
PRIMER_LEFT_4_TM=60.014
PRIMER_RIGHT_4_TM=60.811
PRIMER_INTERNAL_4_TM=59.471
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH1045
SEQUENCE_TEMPLATE=CTGTAGGAAAACCTATTTNNTNATTTCTCGAAATATTTCCTCCCACCCATTTTTNCTGTACTGTCTTCCGAGATNTCCTATTATAGGTTGCACATTCCTAAAATCCTGAAAATCTGAAATCCAAAATGCTCTAAAATCTGAAACTTTTTGAATGCCAACATGATGCCACTAGTGGAAAGCTGAAAGTTTCACACCTGACCTCATGTGATGAGTCGCAGTAGAAACACAGGCACGTAACACAAAGTTTATTCAGCATTTCCAGAGGGAAAAAGATCTTCCCAG
SEQUENCE_INCLUDED_REGION=0,282
P3_COMMENT=CL is TATTTCCTCCCACCCATTTT
P3_COMMENT=CR is TGGGAAGATCTTTTTCCCTC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.529843
PRIMER_LEFT_0_PENALTY=0.044752
PRIMER_RIGHT_0_PENALTY=0.485091
PRIMER_INTERNAL_0_PENALTY=1.013478
PRIMER_LEFT_0_SEQUENCE=ATGCCAACATGATGCCACTA
PRIMER_RIGHT_0_SEQUENCE=GTGCCTGTGTTTCTACTGCG
PRIMER_INTERNAL_0_SEQUENCE=CACACCTGACCTCATGTGATG
PRIMER_LEFT_0=151,20
PRIMER_RIGHT_0=232,20
PRIMER_INTERNAL_0=189,21
PRIMER_LEFT_0_TM=59.955
PRIMER_RIGHT_0_TM=59.515
PRIMER_INTERNAL_0_TM=60.013
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=10.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=0.529843
PRIMER_LEFT_1_PENALTY=0.044752
PRIMER_RIGHT_1_PENALTY=0.485091
PRIMER_INTERNAL_1_PENALTY=1.013478
PRIMER_LEFT_1_SEQUENCE=ATGCCAACATGATGCCACTA
PRIMER_RIGHT_1_SEQUENCE=CGTGCCTGTGTTTCTACTGC
PRIMER_INTERNAL_1_SEQUENCE=CACACCTGACCTCATGTGATG
PRIMER_LEFT_1=151,20
PRIMER_RIGHT_1=233,20
PRIMER_INTERNAL_1=189,21
PRIMER_LEFT_1_TM=59.955
PRIMER_RIGHT_1_TM=59.515
PRIMER_INTERNAL_1_TM=60.013
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.641193
PRIMER_LEFT_2_PENALTY=0.044752
PRIMER_RIGHT_2_PENALTY=0.596442
PRIMER_INTERNAL_2_PENALTY=1.013478
PRIMER_LEFT_2_SEQUENCE=ATGCCAACATGATGCCACTA
PRIMER_RIGHT_2_SEQUENCE=TGCCTGTGTTTCTACTGCGA
PRIMER_INTERNAL_2_SEQUENCE=CACACCTGACCTCATGTGATG
PRIMER_LEFT_2=151,20
PRIMER_RIGHT_2=231,20
PRIMER_INTERNAL_2=189,21
PRIMER_LEFT_2_TM=59.955
PRIMER_RIGHT_2_TM=60.596
PRIMER_INTERNAL_2_TM=60.013
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=8.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=0.960711
PRIMER_LEFT_3_PENALTY=0.475619
PRIMER_RIGHT_3_PENALTY=0.485091
PRIMER_INTERNAL_3_PENALTY=1.013478
PRIMER_LEFT_3_SEQUENCE=TTTGAATGCCAACATGATGC
PRIMER_RIGHT_3_SEQUENCE=GTGCCTGTGTTTCTACTGCG
PRIMER_INTERNAL_3_SEQUENCE=CACACCTGACCTCATGTGATG
PRIMER_LEFT_3=146,20
PRIMER_RIGHT_3=232,20
PRIMER_INTERNAL_3=189,21
PRIMER_LEFT_3_TM=60.476
PRIMER_RIGHT_3_TM=59.515
PRIMER_INTERNAL_3_TM=60.013
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=87
PRIMER_PAIR_4_PENALTY=0.960711
PRIMER_LEFT_4_PENALTY=0.475619
PRIMER_RIGHT_4_PENALTY=0.485091
PRIMER_INTERNAL_4_PENALTY=1.013478
PRIMER_LEFT_4_SEQUENCE=TTTGAATGCCAACATGATGC
PRIMER_RIGHT_4_SEQUENCE=CGTGCCTGTGTTTCTACTGC
PRIMER_INTERNAL_4_SEQUENCE=CACACCTGACCTCATGTGATG
PRIMER_LEFT_4=146,20
PRIMER_RIGHT_4=233,20
PRIMER_INTERNAL_4=189,21
PRIMER_LEFT_4_TM=60.476
PRIMER_RIGHT_4_TM=59.515
PRIMER_INTERNAL_4_TM=60.013
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH1046
SEQUENCE_TEMPLATE=GGAGCCATTCTCCTCGGTCTCGCTGGCCTGCCATGGAGAACACAAGGGCATNNGGTGGCCAGGCCNATGCAGCCAGGCTCCAGGCATCCCCAGGATCTCAGCCCCTCCAAGGGTACCCAGAATATTGAGGCACAGAGAGAAGCGACTGGCCTGAATACATACCTGGCTCCGCATCCCTGTGCCTCTNAGGACCCTAGACTCCCCTGATTCAGCCTT
SEQUENCE_INCLUDED_REGION=0,216
P3_COMMENT=CL is GCCATTCTCCTCGGTCTC
P3_COMMENT=CR is AATCAGGGGAGTCTAGGGTC
P3_COMMENT=CM is GCCTGCCATGGAGAACAC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.238489
PRIMER_LEFT_0_PENALTY=0.022341
PRIMER_RIGHT_0_PENALTY=0.216148
PRIMER_INTERNAL_0_PENALTY=2.185516
PRIMER_LEFT_0_SEQUENCE=AGAAGCGACTGGCCTGAATA
PRIMER_RIGHT_0_SEQUENCE=AGGCTGAATCAGGGGAGTCT
PRIMER_INTERNAL_0_SEQUENCE=CATACCTGGCTCCGCATC
PRIMER_LEFT_0=137,20
PRIMER_RIGHT_0=214,20
PRIMER_INTERNAL_0=157,18
PRIMER_LEFT_0_TM=59.978
PRIMER_RIGHT_0_TM=60.216
PRIMER_INTERNAL_0_TM=60.186
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=0.358199
PRIMER_LEFT_1_PENALTY=0.292425
PRIMER_RIGHT_1_PENALTY=0.065775
PRIMER_INTERNAL_1_PENALTY=0.443661
PRIMER_LEFT_1_SEQUENCE=GAGGCACAGAGAGAAGCGAC
PRIMER_RIGHT_1_SEQUENCE=TCAGGGGAGTCTAGGGTCCT
PRIMER_INTERNAL_1_SEQUENCE=GAATACATACCTGGCTCCGC
PRIMER_LEFT_1=126,20
PRIMER_RIGHT_1=206,20
PRIMER_INTERNAL_1=152,20
PRIMER_LEFT_1_TM=60.292
PRIMER_RIGHT_1_TM=60.066
PRIMER_INTERNAL_1_TM=59.556
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=81
PRIMER_PAIR_2_PENALTY=0.373365
PRIMER_LEFT_2_PENALTY=0.157217
PRIMER_RIGHT_2_PENALTY=0.216148
PRIMER_INTERNAL_2_PENALTY=3.006580
PRIMER_LEFT_2_SEQUENCE=GAAGCGACTGGCCTGAATAC
PRIMER_RIGHT_2_SEQUENCE=AGGCTGAATCAGGGGAGTCT
PRIMER_INTERNAL_2_SEQUENCE=ATACCTGGCTCCGCATCC
PRIMER_LEFT_2=138,20
PRIMER_RIGHT_2=214,20
PRIMER_INTERNAL_2=158,18
PRIMER_LEFT_2_TM=59.843
PRIMER_RIGHT_2_TM=60.216
PRIMER_INTERNAL_2_TM=61.007
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=0.508572
PRIMER_LEFT_3_PENALTY=0.292425
PRIMER_RIGHT_3_PENALTY=0.216148
PRIMER_INTERNAL_3_PENALTY=0.443661
PRIMER_LEFT_3_SEQUENCE=GAGGCACAGAGAGAAGCGAC
PRIMER_RIGHT_3_SEQUENCE=AGGCTGAATCAGGGGAGTCT
PRIMER_INTERNAL_3_SEQUENCE=GAATACATACCTGGCTCCGC
PRIMER_LEFT_3=126,20
PRIMER_RIGHT_3=214,20
PRIMER_INTERNAL_3=152,20
PRIMER_LEFT_3_TM=60.292
PRIMER_RIGHT_3_TM=60.216
PRIMER_INTERNAL_3_TM=59.556
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=0.618163
PRIMER_LEFT_4_PENALTY=0.022341
PRIMER_RIGHT_4_PENALTY=0.595822
PRIMER_INTERNAL_4_PENALTY=2.185516
PRIMER_LEFT_4_SEQUENCE=AGAAGCGACTGGCCTGAATA
PRIMER_RIGHT_4_SEQUENCE=AAGGCTGAATCAGGGGAGTC
PRIMER_INTERNAL_4_SEQUENCE=CATACCTGGCTCCGCATC
PRIMER_LEFT_4=137,20
PRIMER_RIGHT_4=215,20
PRIMER_INTERNAL_4=157,18
PRIMER_LEFT_4_TM=59.978
PRIMER_RIGHT_4_TM=60.596
PRIMER_INTERNAL_4_TM=60.186
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH1049
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAATCAACATGGGATGGCTCAGCTGAAACCAACATGGGGTGGCTTCAGGCCATCCCAGGCATTCAGAGGGTTCGTTTAATGACATTCACTGAGGCCCTGTCTATGTCAGGCCCTTGGTGTTGAAGACGCAATCATGAACAAAAATGAAAATACAATGTGATGGTCTCCTGAGTGTCTGAATGCGCCAGGTGGCTAAGTGCTGGGGCTCTGGGGTCAGGCTGCCTGGGTCACATCCTGGCTCCAAACTGCTTTGCTATGGCTTCGGGCAGTTACCTCATCTGTAAAATGGG
SEQUENCE_INCLUDED_REGION=36,293
P3_COMMENT=CL is GCATTCAGAGGGTTCGTTTA
P3_COMMENT=CR is AGCCATAGCAAAGCAGTTTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.310062
PRIMER_LEFT_0_PENALTY=0.243800
PRIMER_RIGHT_0_PENALTY=0.066262
PRIMER_INTERNAL_0_PENALTY=1.018537
PRIMER_LEFT_0_SEQUENCE=AAATCAACATGGGATGGCTC
PRIMER_RIGHT_0_SEQUENCE=GGGCCTCAGTGAATGTCATT
PRIMER_INTERNAL_0_SEQUENCE=AGGCATTCAGAGGGTTCGTT
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=135,20
PRIMER_INTERNAL_0=95,20
PRIMER_LEFT_0_TM=59.756
PRIMER_RIGHT_0_TM=59.934
PRIMER_INTERNAL_0_TM=61.019
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=7.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=0.310062
PRIMER_LEFT_1_PENALTY=0.243800
PRIMER_RIGHT_1_PENALTY=0.066262
PRIMER_INTERNAL_1_PENALTY=1.018537
PRIMER_LEFT_1_SEQUENCE=GAAATCAACATGGGATGGCT
PRIMER_RIGHT_1_SEQUENCE=GGGCCTCAGTGAATGTCATT
PRIMER_INTERNAL_1_SEQUENCE=AGGCATTCAGAGGGTTCGTT
PRIMER_LEFT_1=38,20
PRIMER_RIGHT_1=135,20
PRIMER_INTERNAL_1=95,20
PRIMER_LEFT_1_TM=59.756
PRIMER_RIGHT_1_TM=59.934
PRIMER_INTERNAL_1_TM=61.019
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=98
PRIMER_PAIR_2_PENALTY=0.329116
PRIMER_LEFT_2_PENALTY=0.262854
PRIMER_RIGHT_2_PENALTY=0.066262
PRIMER_INTERNAL_2_PENALTY=1.018537
PRIMER_LEFT_2_SEQUENCE=GGCTCAGCTGAAACCAACAT
PRIMER_RIGHT_2_SEQUENCE=GGGCCTCAGTGAATGTCATT
PRIMER_INTERNAL_2_SEQUENCE=AGGCATTCAGAGGGTTCGTT
PRIMER_LEFT_2=54,20
PRIMER_RIGHT_2=135,20
PRIMER_INTERNAL_2=95,20
PRIMER_LEFT_2_TM=60.263
PRIMER_RIGHT_2_TM=59.934
PRIMER_INTERNAL_2_TM=61.019
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=7.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=0.329116
PRIMER_LEFT_3_PENALTY=0.262854
PRIMER_RIGHT_3_PENALTY=0.066262
PRIMER_INTERNAL_3_PENALTY=1.018537
PRIMER_LEFT_3_SEQUENCE=ATGGCTCAGCTGAAACCAAC
PRIMER_RIGHT_3_SEQUENCE=GGGCCTCAGTGAATGTCATT
PRIMER_INTERNAL_3_SEQUENCE=AGGCATTCAGAGGGTTCGTT
PRIMER_LEFT_3=52,20
PRIMER_RIGHT_3=135,20
PRIMER_INTERNAL_3=95,20
PRIMER_LEFT_3_TM=60.263
PRIMER_RIGHT_3_TM=59.934
PRIMER_INTERNAL_3_TM=61.019
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=7.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=0.363609
PRIMER_LEFT_4_PENALTY=0.243800
PRIMER_RIGHT_4_PENALTY=0.119809
PRIMER_INTERNAL_4_PENALTY=1.018537
PRIMER_LEFT_4_SEQUENCE=AAATCAACATGGGATGGCTC
PRIMER_RIGHT_4_SEQUENCE=ACAGGGCCTCAGTGAATGTC
PRIMER_INTERNAL_4_SEQUENCE=AGGCATTCAGAGGGTTCGTT
PRIMER_LEFT_4=39,20
PRIMER_RIGHT_4=138,20
PRIMER_INTERNAL_4=95,20
PRIMER_LEFT_4_TM=59.756
PRIMER_RIGHT_4_TM=60.120
PRIMER_INTERNAL_4_TM=61.019
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH105
SEQUENCE_TEMPLATE=AGCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATAAAGGAGAGTATTTTAAAAGTGCCTCCCAGTAGGAAGAACAGTCACAAGGCACTGTTATATCAATTCAGTGTGACACAAGCCCTGATTATTTAATAGTATAACAGCAGTGAATCAGAGTTCTTTCATCTGACTTTGCTGACATTTCCAGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
SEQUENCE_INCLUDED_REGION=35,155
P3_COMMENT=CL is GTAGGAAGAACAGTCACAAGGC
P3_COMMENT=CR is TGGAAATGTCAGCAAAGTCA
P3_COMMENT=CM is TGTTATATCAATTCAGTGTGACACA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.816278
PRIMER_LEFT_0_PENALTY=0.690085
PRIMER_RIGHT_0_PENALTY=1.126193
PRIMER_INTERNAL_0_PENALTY=4.580191
PRIMER_LEFT_0_SEQUENCE=TTCAGTGTGACACAAGCCCT
PRIMER_RIGHT_0_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
PRIMER_INTERNAL_0_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
PRIMER_LEFT_0=104,20
PRIMER_RIGHT_0=189,21
PRIMER_INTERNAL_0=144,24
PRIMER_LEFT_0_TM=59.310
PRIMER_RIGHT_0_TM=59.874
PRIMER_INTERNAL_0_TM=59.420
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=2.064695
PRIMER_LEFT_1_PENALTY=0.938502
PRIMER_RIGHT_1_PENALTY=1.126193
PRIMER_INTERNAL_1_PENALTY=4.580191
PRIMER_LEFT_1_SEQUENCE=CAGTGTGACACAAGCCCTGA
PRIMER_RIGHT_1_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
PRIMER_INTERNAL_1_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
PRIMER_LEFT_1=106,20
PRIMER_RIGHT_1=189,21
PRIMER_INTERNAL_1=144,24
PRIMER_LEFT_1_TM=60.939
PRIMER_RIGHT_1_TM=59.874
PRIMER_INTERNAL_1_TM=59.420
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=2.064695
PRIMER_LEFT_2_PENALTY=0.938502
PRIMER_RIGHT_2_PENALTY=1.126193
PRIMER_INTERNAL_2_PENALTY=4.580191
PRIMER_LEFT_2_SEQUENCE=TCAGTGTGACACAAGCCCTG
PRIMER_RIGHT_2_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
PRIMER_INTERNAL_2_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
PRIMER_LEFT_2=105,20
PRIMER_RIGHT_2=189,21
PRIMER_INTERNAL_2=144,24
PRIMER_LEFT_2_TM=60.939
PRIMER_RIGHT_2_TM=59.874
PRIMER_INTERNAL_2_TM=59.420
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=2.153786
PRIMER_LEFT_3_PENALTY=1.027592
PRIMER_RIGHT_3_PENALTY=1.126193
PRIMER_INTERNAL_3_PENALTY=4.580191
PRIMER_LEFT_3_SEQUENCE=AATTCAGTGTGACACAAGCCC
PRIMER_RIGHT_3_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
PRIMER_INTERNAL_3_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
PRIMER_LEFT_3=102,21
PRIMER_RIGHT_3=189,21
PRIMER_INTERNAL_3=144,24
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=59.874
PRIMER_INTERNAL_3_TM=59.420
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=2.489066
PRIMER_LEFT_4_PENALTY=1.362873
PRIMER_RIGHT_4_PENALTY=1.126193
PRIMER_INTERNAL_4_PENALTY=4.580191
PRIMER_LEFT_4_SEQUENCE=AGTGTGACACAAGCCCTGATT
PRIMER_RIGHT_4_SEQUENCE=GCTGGAAATGTCAGCAAAGTC
PRIMER_INTERNAL_4_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC
PRIMER_LEFT_4=107,21
PRIMER_RIGHT_4=189,21
PRIMER_INTERNAL_4=144,24
PRIMER_LEFT_4_TM=59.637
PRIMER_RIGHT_4_TM=59.874
PRIMER_INTERNAL_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=83
=
SEQUENCE_ID=MH1051
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGTGAACTCATATAATTTTCTTTCCAGCCTGTTACTATACATATATGCATGATGTCCATGCTCTCATCTAAAATCAGCCTCTGCTTGTGTACTAGATCAGGGTTGACAAAATGCAGCCTGTGGCCCAAATCCTGCATTTTGTTTTGTTTTTGTACAGCCCGTAAGCCAAGAATAGTTTTTACTTTTTTTTTTTTTTTTTTTTTTT
SEQUENCE_INCLUDED_REGION=36,207
P3_COMMENT=CL is TATGCATGATGTCCATGCTC
P3_COMMENT=CR is ACTATTCTTGGCTTACGGGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.531471
PRIMER_LEFT_0_PENALTY=0.308722
PRIMER_RIGHT_0_PENALTY=0.222749
PRIMER_INTERNAL_0_PENALTY=0.199246
PRIMER_LEFT_0_SEQUENCE=TCAGGGTTGACAAAATGCAG
PRIMER_RIGHT_0_SEQUENCE=TATTCTTGGCTTACGGGCTG
PRIMER_INTERNAL_0_SEQUENCE=CCCAAATCCTGCATTTTGTT
PRIMER_LEFT_0=134,20
PRIMER_RIGHT_0=211,20
PRIMER_INTERNAL_0=161,20
PRIMER_LEFT_0_TM=59.691
PRIMER_RIGHT_0_TM=60.223
PRIMER_INTERNAL_0_TM=59.801
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=0.579059
PRIMER_LEFT_1_PENALTY=0.308722
PRIMER_RIGHT_1_PENALTY=0.270338
PRIMER_INTERNAL_1_PENALTY=0.199246
PRIMER_LEFT_1_SEQUENCE=TCAGGGTTGACAAAATGCAG
PRIMER_RIGHT_1_SEQUENCE=TCTTGGCTTACGGGCTGTAC
PRIMER_INTERNAL_1_SEQUENCE=CCCAAATCCTGCATTTTGTT
PRIMER_LEFT_1=134,20
PRIMER_RIGHT_1=208,20
PRIMER_INTERNAL_1=161,20
PRIMER_LEFT_1_TM=59.691
PRIMER_RIGHT_1_TM=60.270
PRIMER_INTERNAL_1_TM=59.801
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=1.017068
PRIMER_LEFT_2_PENALTY=0.817822
PRIMER_RIGHT_2_PENALTY=0.199246
PRIMER_INTERNAL_2_PENALTY=0.308722
PRIMER_LEFT_2_SEQUENCE=GCATGATGTCCATGCTCTCA
PRIMER_RIGHT_2_SEQUENCE=AACAAAATGCAGGATTTGGG
PRIMER_INTERNAL_2_SEQUENCE=TCAGGGTTGACAAAATGCAG
PRIMER_LEFT_2=84,20
PRIMER_RIGHT_2=180,20
PRIMER_INTERNAL_2=134,20
PRIMER_LEFT_2_TM=60.818
PRIMER_RIGHT_2_TM=59.801
PRIMER_INTERNAL_2_TM=59.691
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=1.017068
PRIMER_LEFT_3_PENALTY=0.817822
PRIMER_RIGHT_3_PENALTY=0.199246
PRIMER_INTERNAL_3_PENALTY=0.308722
PRIMER_LEFT_3_SEQUENCE=TGCATGATGTCCATGCTCTC
PRIMER_RIGHT_3_SEQUENCE=AACAAAATGCAGGATTTGGG
PRIMER_INTERNAL_3_SEQUENCE=TCAGGGTTGACAAAATGCAG
PRIMER_LEFT_3=83,20
PRIMER_RIGHT_3=180,20
PRIMER_INTERNAL_3=134,20
PRIMER_LEFT_3_TM=60.818
PRIMER_RIGHT_3_TM=59.801
PRIMER_INTERNAL_3_TM=59.691
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=1.070958
PRIMER_LEFT_4_PENALTY=0.308722
PRIMER_RIGHT_4_PENALTY=0.762236
PRIMER_INTERNAL_4_PENALTY=0.199246
PRIMER_LEFT_4_SEQUENCE=TCAGGGTTGACAAAATGCAG
PRIMER_RIGHT_4_SEQUENCE=TTCTTGGCTTACGGGCTGTA
PRIMER_INTERNAL_4_SEQUENCE=CCCAAATCCTGCATTTTGTT
PRIMER_LEFT_4=134,20
PRIMER_RIGHT_4=209,20
PRIMER_INTERNAL_4=161,20
PRIMER_LEFT_4_TM=59.691
PRIMER_RIGHT_4_TM=60.762
PRIMER_INTERNAL_4_TM=59.801
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1055
SEQUENCE_TEMPLATE=TGCNTGCAGGTCGACTCTAGAGGATCCCCCTAACATTCCAGTGGAGGAACTAAAACTATTATCAAGTGACCAAATAAATGAGCAAGATTGTTTCAGAGAATGAAGTCTGTGGGGGAAAATAATAGGTTAGAGTGATCAGAGACAGAGAACTGGGTGGATAAAGGGGGCAGGAGAGACGTTGCTAGAAACCACTGTGTACGTTGAACATATTCTGACTCTGATCCTCCCAATGGAAGACCCTTATTCTTATTGCTTCTTCTTCTTTGTCTCCCCAGCCCCTGGCACAGAGTGAGCACTTAAAAACCACCTGTGTACATATGACTGACCTTTCCGTCACCTGCCTTCCCATTGGCCTCTTCCTGCCCCTTCATCCACCTTTGTGTTTGAATATTCATTCACATGGCTTGTTCTTC
SEQUENCE_INCLUDED_REGION=29,384
P3_COMMENT=CL is AACATTCCAGTGGAGGAACT
P3_COMMENT=CR is GAACAAGCCATGTGAATGAA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.253180
PRIMER_LEFT_0_PENALTY=0.096893
PRIMER_RIGHT_0_PENALTY=0.156287
PRIMER_INTERNAL_0_PENALTY=1.115240
PRIMER_LEFT_0_SEQUENCE=GAATGAAGTCTGTGGGGGAA
PRIMER_RIGHT_0_SEQUENCE=CTAGCAACGTCTCTCCTGCC
PRIMER_INTERNAL_0_SEQUENCE=CAGAGACAGAGAACTGGGTGG
PRIMER_LEFT_0=97,20
PRIMER_RIGHT_0=184,20
PRIMER_INTERNAL_0=136,21
PRIMER_LEFT_0_TM=59.903
PRIMER_RIGHT_0_TM=60.156
PRIMER_INTERNAL_0_TM=59.885
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=57.143
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.467584
PRIMER_LEFT_1_PENALTY=0.464275
PRIMER_RIGHT_1_PENALTY=0.003309
PRIMER_INTERNAL_1_PENALTY=3.012411
PRIMER_LEFT_1_SEQUENCE=ATAAAGGGGGCAGGAGAGAC
PRIMER_RIGHT_1_SEQUENCE=TTCCATTGGGAGGATCAGAG
PRIMER_INTERNAL_1_SEQUENCE=GAAACCACTGTGTACGTTGAACA
PRIMER_LEFT_1=157,20
PRIMER_RIGHT_1=234,20
PRIMER_INTERNAL_1=184,23
PRIMER_LEFT_1_TM=59.536
PRIMER_RIGHT_1_TM=60.003
PRIMER_INTERNAL_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=0.467584
PRIMER_LEFT_2_PENALTY=0.464275
PRIMER_RIGHT_2_PENALTY=0.003309
PRIMER_INTERNAL_2_PENALTY=3.012411
PRIMER_LEFT_2_SEQUENCE=ATAAAGGGGGCAGGAGAGAC
PRIMER_RIGHT_2_SEQUENCE=CTTCCATTGGGAGGATCAGA
PRIMER_INTERNAL_2_SEQUENCE=GAAACCACTGTGTACGTTGAACA
PRIMER_LEFT_2=157,20
PRIMER_RIGHT_2=235,20
PRIMER_INTERNAL_2=184,23
PRIMER_LEFT_2_TM=59.536
PRIMER_RIGHT_2_TM=60.003
PRIMER_INTERNAL_2_TM=59.988
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=0.467584
PRIMER_LEFT_3_PENALTY=0.464275
PRIMER_RIGHT_3_PENALTY=0.003309
PRIMER_INTERNAL_3_PENALTY=3.012411
PRIMER_LEFT_3_SEQUENCE=ATAAAGGGGGCAGGAGAGAC
PRIMER_RIGHT_3_SEQUENCE=TCTTCCATTGGGAGGATCAG
PRIMER_INTERNAL_3_SEQUENCE=GAAACCACTGTGTACGTTGAACA
PRIMER_LEFT_3=157,20
PRIMER_RIGHT_3=236,20
PRIMER_INTERNAL_3=184,23
PRIMER_LEFT_3_TM=59.536
PRIMER_RIGHT_3_TM=60.003
PRIMER_INTERNAL_3_TM=59.988
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=0.540641
PRIMER_LEFT_4_PENALTY=0.537332
PRIMER_RIGHT_4_PENALTY=0.003309
PRIMER_INTERNAL_4_PENALTY=3.012411
PRIMER_LEFT_4_SEQUENCE=GATAAAGGGGGCAGGAGAGA
PRIMER_RIGHT_4_SEQUENCE=TTCCATTGGGAGGATCAGAG
PRIMER_INTERNAL_4_SEQUENCE=GAAACCACTGTGTACGTTGAACA
PRIMER_LEFT_4=156,20
PRIMER_RIGHT_4=234,20
PRIMER_INTERNAL_4=184,23
PRIMER_LEFT_4_TM=60.537
PRIMER_RIGHT_4_TM=60.003
PRIMER_INTERNAL_4_TM=59.988
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH1056
SEQUENCE_TEMPLATE=NNTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTGTGTATTGAGGGAAGGGGAGATGTGTGCACACNTTCCAGAAAAATGGGGAGGTAGTTTAGAAAGTNTTTCTCTGTAACAGCACAG
SEQUENCE_INCLUDED_REGION=36,89
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH1058
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCAATTTATAGGAAGTGAGGACAGGATAGCATGTTAAAGGGTATGGATGGCTTATAGGAGAGGGAAGGTTGAACTGAGTCCCCTGAAAGAGACCTGAGAAACATATCTACCAAATACAATGTTAACTTTGTTGGGATTCTGGTCCAAAGAAACGTACTAAAAAATATGGGGAGAAATAGAGGANTATGAATATGGCCTGAGTACTATAAGATATTAAAGAAAAATTATTAACTTGCTAGGTATGATAATGATATTTTGGTTATGTTTTTTAAAAAGTTGTTAAAAATACATAGTGAAGTACTTCAGGTGA
SEQUENCE_INCLUDED_REGION=36,313
P3_COMMENT=CL is TAGGAAGTGAGGACAGGATAGC
P3_COMMENT=CR is TCTTATAGTACTCAGGCCATATTCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.518143
PRIMER_LEFT_0_PENALTY=0.088170
PRIMER_RIGHT_0_PENALTY=0.429973
PRIMER_INTERNAL_0_PENALTY=6.837090
PRIMER_LEFT_0_SEQUENCE=GGTTGAACTGAGTCCCCTGA
PRIMER_RIGHT_0_SEQUENCE=TCTTTGGACCAGAATCCCAA
PRIMER_INTERNAL_0_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
PRIMER_LEFT_0=105,20
PRIMER_RIGHT_0=188,20
PRIMER_INTERNAL_0=127,26
PRIMER_LEFT_0_TM=60.088
PRIMER_RIGHT_0_TM=60.430
PRIMER_INTERNAL_0_TM=59.163
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=38.462
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=0.518143
PRIMER_LEFT_1_PENALTY=0.088170
PRIMER_RIGHT_1_PENALTY=0.429973
PRIMER_INTERNAL_1_PENALTY=6.837090
PRIMER_LEFT_1_SEQUENCE=GGTTGAACTGAGTCCCCTGA
PRIMER_RIGHT_1_SEQUENCE=TTCTTTGGACCAGAATCCCA
PRIMER_INTERNAL_1_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
PRIMER_LEFT_1=105,20
PRIMER_RIGHT_1=189,20
PRIMER_INTERNAL_1=127,26
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=60.430
PRIMER_INTERNAL_1_TM=59.163
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=38.462
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=0.707220
PRIMER_LEFT_2_PENALTY=0.088170
PRIMER_RIGHT_2_PENALTY=0.619050
PRIMER_INTERNAL_2_PENALTY=6.837090
PRIMER_LEFT_2_SEQUENCE=GGTTGAACTGAGTCCCCTGA
PRIMER_RIGHT_2_SEQUENCE=GGACCAGAATCCCAACAAAG
PRIMER_INTERNAL_2_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
PRIMER_LEFT_2=105,20
PRIMER_RIGHT_2=183,20
PRIMER_INTERNAL_2=127,26
PRIMER_LEFT_2_TM=60.088
PRIMER_RIGHT_2_TM=59.381
PRIMER_INTERNAL_2_TM=59.163
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=38.462
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=0.707220
PRIMER_LEFT_3_PENALTY=0.088170
PRIMER_RIGHT_3_PENALTY=0.619050
PRIMER_INTERNAL_3_PENALTY=6.837090
PRIMER_LEFT_3_SEQUENCE=GGTTGAACTGAGTCCCCTGA
PRIMER_RIGHT_3_SEQUENCE=CTTTGGACCAGAATCCCAAC
PRIMER_INTERNAL_3_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
PRIMER_LEFT_3=105,20
PRIMER_RIGHT_3=187,20
PRIMER_INTERNAL_3=127,26
PRIMER_LEFT_3_TM=60.088
PRIMER_RIGHT_3_TM=59.381
PRIMER_INTERNAL_3_TM=59.163
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=38.462
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=1.131882
PRIMER_LEFT_4_PENALTY=0.088170
PRIMER_RIGHT_4_PENALTY=1.043712
PRIMER_INTERNAL_4_PENALTY=6.837090
PRIMER_LEFT_4_SEQUENCE=GGTTGAACTGAGTCCCCTGA
PRIMER_RIGHT_4_SEQUENCE=TTTCTTTGGACCAGAATCCC
PRIMER_INTERNAL_4_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA
PRIMER_LEFT_4=105,20
PRIMER_RIGHT_4=190,20
PRIMER_INTERNAL_4=127,26
PRIMER_LEFT_4_TM=60.088
PRIMER_RIGHT_4_TM=58.956
PRIMER_INTERNAL_4_TM=59.163
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=38.462
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH1059
SEQUENCE_TEMPLATE=GGTGGATGCTNAGGTCGACTCTAGAGGATCCCCCTTATCTATTTACTTTTGCTACTTAAGTAAATAACTTATCACATCATAAGTGCATTGCNTTTGANGGTTTCAGTTGGTTGGCTTAGCACATGGTAAACACCAATTNGGCATTCTCTCTTGTNNAGTNGCCATTTGCAAAATGNATTATTTCAGATTTGCTGAAATACTGAATTTGTAAGTTTACACTCTGCTTATTCATATTTNTAAATAACTCTTTTGCCATTNGGTCCCAAACCCATGTGCTGATGTGTCTGCNGGGTCTCATTTCTTCCCCTGTATTAGTATCTGA
SEQUENCE_INCLUDED_REGION=33,289
P3_COMMENT=CL is TTTCAGTTGGTTGGCTTAGC
P3_COMMENT=CR is ACAGGGGAAGAAATGAGACC
P3_COMMENT=CM is TATTTCAGATTTGCTGAAATACTGA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.757764
PRIMER_LEFT_0_PENALTY=6.805269
PRIMER_RIGHT_0_PENALTY=1.952496
PRIMER_INTERNAL_0_PENALTY=9.929484
PRIMER_LEFT_0_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
PRIMER_RIGHT_0_SEQUENCE=CATCAGCACATGGGTTTGG
PRIMER_INTERNAL_0_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
PRIMER_LEFT_0=181,24
PRIMER_RIGHT_0=280,19
PRIMER_INTERNAL_0=205,27
PRIMER_LEFT_0_TM=57.195
PRIMER_RIGHT_0_TM=60.952
PRIMER_INTERNAL_0_TM=57.071
PRIMER_LEFT_0_GC_PERCENT=29.167
PRIMER_RIGHT_0_GC_PERCENT=52.632
PRIMER_INTERNAL_0_GC_PERCENT=29.630
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=9.486970
PRIMER_LEFT_1_PENALTY=6.805269
PRIMER_RIGHT_1_PENALTY=2.681702
PRIMER_INTERNAL_1_PENALTY=9.929484
PRIMER_LEFT_1_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
PRIMER_RIGHT_1_SEQUENCE=AGCACATGGGTTTGGGAC
PRIMER_INTERNAL_1_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
PRIMER_LEFT_1=181,24
PRIMER_RIGHT_1=276,18
PRIMER_INTERNAL_1=205,27
PRIMER_LEFT_1_TM=57.195
PRIMER_RIGHT_1_TM=59.318
PRIMER_INTERNAL_1_TM=57.071
PRIMER_LEFT_1_GC_PERCENT=29.167
PRIMER_RIGHT_1_GC_PERCENT=55.556
PRIMER_INTERNAL_1_GC_PERCENT=29.630
PRIMER_LEFT_1_SELF_ANY=11.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=9.556564
PRIMER_LEFT_2_PENALTY=6.805269
PRIMER_RIGHT_2_PENALTY=2.751295
PRIMER_INTERNAL_2_PENALTY=9.929484
PRIMER_LEFT_2_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
PRIMER_RIGHT_2_SEQUENCE=ATCAGCACATGGGTTTGGG
PRIMER_INTERNAL_2_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
PRIMER_LEFT_2=181,24
PRIMER_RIGHT_2=279,19
PRIMER_INTERNAL_2=205,27
PRIMER_LEFT_2_TM=57.195
PRIMER_RIGHT_2_TM=61.751
PRIMER_INTERNAL_2_TM=57.071
PRIMER_LEFT_2_GC_PERCENT=29.167
PRIMER_RIGHT_2_GC_PERCENT=52.632
PRIMER_INTERNAL_2_GC_PERCENT=29.630
PRIMER_LEFT_2_SELF_ANY=11.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=10.184613
PRIMER_LEFT_3_PENALTY=6.805269
PRIMER_RIGHT_3_PENALTY=3.379345
PRIMER_INTERNAL_3_PENALTY=9.929484
PRIMER_LEFT_3_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
PRIMER_RIGHT_3_SEQUENCE=CAGCACATGGGTTTGGGAC
PRIMER_INTERNAL_3_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
PRIMER_LEFT_3=181,24
PRIMER_RIGHT_3=277,19
PRIMER_INTERNAL_3=205,27
PRIMER_LEFT_3_TM=57.195
PRIMER_RIGHT_3_TM=62.379
PRIMER_INTERNAL_3_TM=57.071
PRIMER_LEFT_3_GC_PERCENT=29.167
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_INTERNAL_3_GC_PERCENT=29.630
PRIMER_LEFT_3_SELF_ANY=11.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=7.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=10.349774
PRIMER_LEFT_4_PENALTY=6.805269
PRIMER_RIGHT_4_PENALTY=3.544505
PRIMER_INTERNAL_4_PENALTY=9.929484
PRIMER_LEFT_4_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT
PRIMER_RIGHT_4_SEQUENCE=CAGCACATGGGTTTGGGA
PRIMER_INTERNAL_4_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT
PRIMER_LEFT_4=181,24
PRIMER_RIGHT_4=277,18
PRIMER_INTERNAL_4=205,27
PRIMER_LEFT_4_TM=57.195
PRIMER_RIGHT_4_TM=61.545
PRIMER_INTERNAL_4_TM=57.071
PRIMER_LEFT_4_GC_PERCENT=29.167
PRIMER_RIGHT_4_GC_PERCENT=55.556
PRIMER_INTERNAL_4_GC_PERCENT=29.630
PRIMER_LEFT_4_SELF_ANY=11.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=7.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=97
=
SEQUENCE_ID=MH1064
SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGCTGTCAGTTTGCCACAGTTCTCACCATTCCCTAATCTATACCGCCCCTGCTTCATTCTTCATTCACTACTTGTGAGCAGACATCTGAGATGACTCTGGATTTATGTTTTAATCTCTTTATTTAAAACACACACACATACACTGCACACACACAAGTCACCACAATCACAACCACAAAGAAGTGCTTGAGTATGCTATAGCATGACCTTTTCTTCACATCTCTGCTCAAATATCTCCTTATCAGAGAGGCCTTCTCTTACTACCTTCTAAAATAGGACCCACTGCCCCTCTATGGGTCTCATTTCTCTTCACAGTACTCCAACACTTGACCCATTACTGATTCACTTGTCTACTCT
SEQUENCE_INCLUDED_REGION=37,358
P3_COMMENT=CL is TTCCCTAATCTATACCGCCC
P3_COMMENT=CR is CAGTAATGGGTCAAGTGTTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.696726
PRIMER_LEFT_0_PENALTY=0.273747
PRIMER_RIGHT_0_PENALTY=2.422979
PRIMER_INTERNAL_0_PENALTY=0.048570
PRIMER_LEFT_0_SEQUENCE=GTTTGCCACAGTTCTCACCA
PRIMER_RIGHT_0_SEQUENCE=TCATCTCAGATGTCTGCTCACA
PRIMER_INTERNAL_0_SEQUENCE=AATCTATACCGCCCCTGCTT
PRIMER_LEFT_0=46,20
PRIMER_RIGHT_0=131,22
PRIMER_INTERNAL_0=72,20
PRIMER_LEFT_0_TM=59.726
PRIMER_RIGHT_0_TM=59.577
PRIMER_INTERNAL_0_TM=59.951
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=9.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=2.740914
PRIMER_LEFT_1_PENALTY=0.317935
PRIMER_RIGHT_1_PENALTY=2.422979
PRIMER_INTERNAL_1_PENALTY=0.048570
PRIMER_LEFT_1_SEQUENCE=TGCCACAGTTCTCACCATTC
PRIMER_RIGHT_1_SEQUENCE=TCATCTCAGATGTCTGCTCACA
PRIMER_INTERNAL_1_SEQUENCE=AATCTATACCGCCCCTGCTT
PRIMER_LEFT_1=49,20
PRIMER_RIGHT_1=131,22
PRIMER_INTERNAL_1=72,20
PRIMER_LEFT_1_TM=59.682
PRIMER_RIGHT_1_TM=59.577
PRIMER_INTERNAL_1_TM=59.951
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=9.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=2.785968
PRIMER_LEFT_2_PENALTY=0.362989
PRIMER_RIGHT_2_PENALTY=2.422979
PRIMER_INTERNAL_2_PENALTY=0.048570
PRIMER_LEFT_2_SEQUENCE=CCACAGTTCTCACCATTCCC
PRIMER_RIGHT_2_SEQUENCE=TCATCTCAGATGTCTGCTCACA
PRIMER_INTERNAL_2_SEQUENCE=AATCTATACCGCCCCTGCTT
PRIMER_LEFT_2=51,20
PRIMER_RIGHT_2=131,22
PRIMER_INTERNAL_2=72,20
PRIMER_LEFT_2_TM=60.363
PRIMER_RIGHT_2_TM=59.577
PRIMER_INTERNAL_2_TM=59.951
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=9.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=2.933091
PRIMER_LEFT_3_PENALTY=0.510112
PRIMER_RIGHT_3_PENALTY=2.422979
PRIMER_INTERNAL_3_PENALTY=0.048570
PRIMER_LEFT_3_SEQUENCE=TGCTGTCAGTTTGCCACAGT
PRIMER_RIGHT_3_SEQUENCE=TCATCTCAGATGTCTGCTCACA
PRIMER_INTERNAL_3_SEQUENCE=AATCTATACCGCCCCTGCTT
PRIMER_LEFT_3=38,20
PRIMER_RIGHT_3=131,22
PRIMER_INTERNAL_3=72,20
PRIMER_LEFT_3_TM=60.510
PRIMER_RIGHT_3_TM=59.577
PRIMER_INTERNAL_3_TM=59.951
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=9.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=2.942891
PRIMER_LEFT_4_PENALTY=0.519913
PRIMER_RIGHT_4_PENALTY=2.422979
PRIMER_INTERNAL_4_PENALTY=0.048570
PRIMER_LEFT_4_SEQUENCE=GCCACAGTTCTCACCATTCC
PRIMER_RIGHT_4_SEQUENCE=TCATCTCAGATGTCTGCTCACA
PRIMER_INTERNAL_4_SEQUENCE=AATCTATACCGCCCCTGCTT
PRIMER_LEFT_4=50,20
PRIMER_RIGHT_4=131,22
PRIMER_INTERNAL_4=72,20
PRIMER_LEFT_4_TM=60.520
PRIMER_RIGHT_4_TM=59.577
PRIMER_INTERNAL_4_TM=59.951
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=9.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH1065
SEQUENCE_TEMPLATE=ATGCCGAGATTTGTTTTAAAGTTGGAATAGGAAAATTCCTTCTCTAACATATTTTATACCCCCTGTGGATGACCCTATACCTTACTGTCTAAATNTTGACTTTCTGAGAGTNNGAGGNNGCACTGCTGTTAATGACAATTAGATTGGAGATAAAATCCAAGACTCTTACAGNCAAACTTGGACACATGTCACCCTACCTATGAAGTGGGAATTAATACTGACCTAAAGACGATGATAGTATCAAGAACGAGGTCACGGGTTTTAGAGCAACTAG
SEQUENCE_INCLUDED_REGION=0,274
P3_COMMENT=CL is TTTATACCCCCTGTGGATGA
P3_COMMENT=CR is CCTCGTTCTTGATACTATCATCG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.794957
PRIMER_LEFT_0_PENALTY=0.765704
PRIMER_RIGHT_0_PENALTY=0.029253
PRIMER_INTERNAL_0_PENALTY=5.798652
PRIMER_LEFT_0_SEQUENCE=TGGACACATGTCACCCTACC
PRIMER_RIGHT_0_SEQUENCE=TAAAACCCGTGACCTCGTTC
PRIMER_INTERNAL_0_SEQUENCE=TGAAGTGGGAATTAATACTGACC
PRIMER_LEFT_0=178,20
PRIMER_RIGHT_0=263,20
PRIMER_INTERNAL_0=200,23
PRIMER_LEFT_0_TM=59.234
PRIMER_RIGHT_0_TM=59.971
PRIMER_INTERNAL_0_TM=57.201
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=0.876096
PRIMER_LEFT_1_PENALTY=0.765704
PRIMER_RIGHT_1_PENALTY=0.110392
PRIMER_INTERNAL_1_PENALTY=5.798652
PRIMER_LEFT_1_SEQUENCE=TGGACACATGTCACCCTACC
PRIMER_RIGHT_1_SEQUENCE=TTGCTCTAAAACCCGTGACC
PRIMER_INTERNAL_1_SEQUENCE=TGAAGTGGGAATTAATACTGACC
PRIMER_LEFT_1=178,20
PRIMER_RIGHT_1=269,20
PRIMER_INTERNAL_1=200,23
PRIMER_LEFT_1_TM=59.234
PRIMER_RIGHT_1_TM=60.110
PRIMER_INTERNAL_1_TM=57.201
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=92
PRIMER_PAIR_2_PENALTY=1.031419
PRIMER_LEFT_2_PENALTY=0.765704
PRIMER_RIGHT_2_PENALTY=0.265715
PRIMER_INTERNAL_2_PENALTY=5.798652
PRIMER_LEFT_2_SEQUENCE=TGGACACATGTCACCCTACC
PRIMER_RIGHT_2_SEQUENCE=TGCTCTAAAACCCGTGACCT
PRIMER_INTERNAL_2_SEQUENCE=TGAAGTGGGAATTAATACTGACC
PRIMER_LEFT_2=178,20
PRIMER_RIGHT_2=268,20
PRIMER_INTERNAL_2=200,23
PRIMER_LEFT_2_TM=59.234
PRIMER_RIGHT_2_TM=59.734
PRIMER_INTERNAL_2_TM=57.201
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_3_PENALTY=1.037384
PRIMER_LEFT_3_PENALTY=0.765704
PRIMER_RIGHT_3_PENALTY=0.271680
PRIMER_INTERNAL_3_PENALTY=5.798652
PRIMER_LEFT_3_SEQUENCE=TGGACACATGTCACCCTACC
PRIMER_RIGHT_3_SEQUENCE=CTCTAAAACCCGTGACCTCG
PRIMER_INTERNAL_3_SEQUENCE=TGAAGTGGGAATTAATACTGACC
PRIMER_LEFT_3=178,20
PRIMER_RIGHT_3=266,20
PRIMER_INTERNAL_3=200,23
PRIMER_LEFT_3_TM=59.234
PRIMER_RIGHT_3_TM=59.728
PRIMER_INTERNAL_3_TM=57.201
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=1.173645
PRIMER_LEFT_4_PENALTY=0.765704
PRIMER_RIGHT_4_PENALTY=0.407941
PRIMER_INTERNAL_4_PENALTY=5.798652
PRIMER_LEFT_4_SEQUENCE=TGGACACATGTCACCCTACC
PRIMER_RIGHT_4_SEQUENCE=TCTAAAACCCGTGACCTCGT
PRIMER_INTERNAL_4_SEQUENCE=TGAAGTGGGAATTAATACTGACC
PRIMER_LEFT_4=178,20
PRIMER_RIGHT_4=265,20
PRIMER_INTERNAL_4=200,23
PRIMER_LEFT_4_TM=59.234
PRIMER_RIGHT_4_TM=59.592
PRIMER_INTERNAL_4_TM=57.201
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH1066
SEQUENCE_TEMPLATE=NTNGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTCAATTTCTTTACTGCCTTCAAAAATTAATTCAATCTGTTGAGCATGAATTACCTTATGATCCCCTTTTGTATTGTTCTATCAATGTGATCTGTATCTCATGATGTTCAATTTTTCCACTGCACACACAGNCCCAGTCTAAATAAATGCTGGCTGACTAACTTTCTGCTCACCTCTTGCTGCTCAGATCTTTCTTAAAGAAAGGTTTTGCCATTCAGTTCCAAGGAGCACTATTGGAG
SEQUENCE_INCLUDED_REGION=35,242
P3_COMMENT=CL is TTCAATTTCTTTACTGCCTTCA
P3_COMMENT=CR is GTGCTCCTTGGAACTGAATG
P3_COMMENT=CM is AAATTAATTCAATCTGTTGAGCATG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.701124
PRIMER_LEFT_0_PENALTY=1.141878
PRIMER_RIGHT_0_PENALTY=0.559246
PRIMER_INTERNAL_0_PENALTY=2.010077
PRIMER_LEFT_0_SEQUENCE=TCAATTTTTCCACTGCACACA
PRIMER_RIGHT_0_SEQUENCE=TCTGAGCAGCAAGAGGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
PRIMER_LEFT_0=145,21
PRIMER_RIGHT_0=225,20
PRIMER_INTERNAL_0=170,22
PRIMER_LEFT_0_TM=60.142
PRIMER_RIGHT_0_TM=59.441
PRIMER_INTERNAL_0_TM=59.990
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=1.828134
PRIMER_LEFT_1_PENALTY=1.141878
PRIMER_RIGHT_1_PENALTY=0.686256
PRIMER_INTERNAL_1_PENALTY=2.010077
PRIMER_LEFT_1_SEQUENCE=TCAATTTTTCCACTGCACACA
PRIMER_RIGHT_1_SEQUENCE=CAGCAAGAGGTGAGCAGAAA
PRIMER_INTERNAL_1_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
PRIMER_LEFT_1=145,21
PRIMER_RIGHT_1=219,20
PRIMER_INTERNAL_1=170,22
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=59.314
PRIMER_INTERNAL_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=2.254461
PRIMER_LEFT_2_PENALTY=1.695215
PRIMER_RIGHT_2_PENALTY=0.559246
PRIMER_INTERNAL_2_PENALTY=2.010077
PRIMER_LEFT_2_SEQUENCE=TTTTTCCACTGCACACACAG
PRIMER_RIGHT_2_SEQUENCE=TCTGAGCAGCAAGAGGTGAG
PRIMER_INTERNAL_2_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
PRIMER_LEFT_2=149,20
PRIMER_RIGHT_2=225,20
PRIMER_INTERNAL_2=170,22
PRIMER_LEFT_2_TM=58.305
PRIMER_RIGHT_2_TM=59.441
PRIMER_INTERNAL_2_TM=59.990
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=2.364539
PRIMER_LEFT_3_PENALTY=0.686256
PRIMER_RIGHT_3_PENALTY=1.678283
PRIMER_INTERNAL_3_PENALTY=2.271131
PRIMER_LEFT_3_SEQUENCE=TTTCTGCTCACCTCTTGCTG
PRIMER_RIGHT_3_SEQUENCE=CTCCAATAGTGCTCCTTGGAA
PRIMER_INTERNAL_3_SEQUENCE=AAAGAAAGGTTTTGCCATTCA
PRIMER_LEFT_3=200,20
PRIMER_RIGHT_3=276,21
PRIMER_INTERNAL_3=234,21
PRIMER_LEFT_3_TM=59.314
PRIMER_RIGHT_3_TM=59.322
PRIMER_INTERNAL_3_TM=58.729
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=2.381854
PRIMER_LEFT_4_PENALTY=1.141878
PRIMER_RIGHT_4_PENALTY=1.239976
PRIMER_INTERNAL_4_PENALTY=2.010077
PRIMER_LEFT_4_SEQUENCE=TCAATTTTTCCACTGCACACA
PRIMER_RIGHT_4_SEQUENCE=ATCTGAGCAGCAAGAGGTGAG
PRIMER_INTERNAL_4_SEQUENCE=CCCAGTCTAAATAAATGCTGGC
PRIMER_LEFT_4=145,21
PRIMER_RIGHT_4=226,21
PRIMER_INTERNAL_4=170,22
PRIMER_LEFT_4_TM=60.142
PRIMER_RIGHT_4_TM=59.760
PRIMER_INTERNAL_4_TM=59.990
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH107
SEQUENCE_TEMPLATE=GCATTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTCCTTCTNACCCTGAACCAGGAAACTCACCGGCAGGGAATCAAGATGGGAAGTAAAAAGAAAATATTCCCCTAAGTCAGGNTCAGATTCTATCATGCCTGGCCACCTGGAGAAGATAGTTGGGTTTAGGGNNCAGAAGAGAAGGGNTTAGNTGNNCAGANCACANCCTNATCANTGTACATCCNCAG
SEQUENCE_INCLUDED_REGION=36,188
P3_COMMENT=CL is ACCCTGAACCAGGAAACTCA
P3_COMMENT=CR is ACTATCTTCTCCAGGTGGCC
P3_COMMENT=CM is CGGCAGGGAATCAAGATG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.527440
PRIMER_LEFT_0_PENALTY=0.450843
PRIMER_RIGHT_0_PENALTY=0.076597
PRIMER_INTERNAL_0_PENALTY=0.521793
PRIMER_LEFT_0_SEQUENCE=CCTGAACCAGGAAACTCACC
PRIMER_RIGHT_0_SEQUENCE=GTGGCCAGGCATGATAGAAT
PRIMER_INTERNAL_0_SEQUENCE=CAGGGAATCAAGATGGGAAG
PRIMER_LEFT_0=47,20
PRIMER_RIGHT_0=141,20
PRIMER_INTERNAL_0=69,20
PRIMER_LEFT_0_TM=59.549
PRIMER_RIGHT_0_TM=59.923
PRIMER_INTERNAL_0_TM=59.478
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=0.527440
PRIMER_LEFT_1_PENALTY=0.450843
PRIMER_RIGHT_1_PENALTY=0.076597
PRIMER_INTERNAL_1_PENALTY=0.521793
PRIMER_LEFT_1_SEQUENCE=CCCTGAACCAGGAAACTCAC
PRIMER_RIGHT_1_SEQUENCE=GTGGCCAGGCATGATAGAAT
PRIMER_INTERNAL_1_SEQUENCE=CAGGGAATCAAGATGGGAAG
PRIMER_LEFT_1=46,20
PRIMER_RIGHT_1=141,20
PRIMER_INTERNAL_1=69,20
PRIMER_LEFT_1_TM=59.549
PRIMER_RIGHT_1_TM=59.923
PRIMER_INTERNAL_1_TM=59.478
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=0.527440
PRIMER_LEFT_2_PENALTY=0.450843
PRIMER_RIGHT_2_PENALTY=0.076597
PRIMER_INTERNAL_2_PENALTY=0.521793
PRIMER_LEFT_2_SEQUENCE=ACCCTGAACCAGGAAACTCA
PRIMER_RIGHT_2_SEQUENCE=GTGGCCAGGCATGATAGAAT
PRIMER_INTERNAL_2_SEQUENCE=CAGGGAATCAAGATGGGAAG
PRIMER_LEFT_2=45,20
PRIMER_RIGHT_2=141,20
PRIMER_INTERNAL_2=69,20
PRIMER_LEFT_2_TM=59.549
PRIMER_RIGHT_2_TM=59.923
PRIMER_INTERNAL_2_TM=59.478
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=0.731663
PRIMER_LEFT_3_PENALTY=0.521793
PRIMER_RIGHT_3_PENALTY=0.209870
PRIMER_INTERNAL_3_PENALTY=5.617642
PRIMER_LEFT_3_SEQUENCE=CAGGGAATCAAGATGGGAAG
PRIMER_RIGHT_3_SEQUENCE=TATCTTCTCCAGGTGGCCAG
PRIMER_INTERNAL_3_SEQUENCE=AAAGAAAATATTCCCCTAAGTCAGG
PRIMER_LEFT_3=69,20
PRIMER_RIGHT_3=153,20
PRIMER_INTERNAL_3=92,25
PRIMER_LEFT_3_TM=59.478
PRIMER_RIGHT_3_TM=60.210
PRIMER_INTERNAL_3_TM=59.382
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=36.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=0.731663
PRIMER_LEFT_4_PENALTY=0.521793
PRIMER_RIGHT_4_PENALTY=0.209870
PRIMER_INTERNAL_4_PENALTY=5.617642
PRIMER_LEFT_4_SEQUENCE=CAGGGAATCAAGATGGGAAG
PRIMER_RIGHT_4_SEQUENCE=CTATCTTCTCCAGGTGGCCA
PRIMER_INTERNAL_4_SEQUENCE=AAAGAAAATATTCCCCTAAGTCAGG
PRIMER_LEFT_4=69,20
PRIMER_RIGHT_4=154,20
PRIMER_INTERNAL_4=92,25
PRIMER_LEFT_4_TM=59.478
PRIMER_RIGHT_4_TM=60.210
PRIMER_INTERNAL_4_TM=59.382
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=36.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH1070
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGCTATATAGCCAAGTCGGTATTTATANNNTGTTTCCACCCTTGATAATTTCATGCCAGTACTCATCAGAATGGAACACTGACTTGGCTCTGTCTGTCCTTATTCTCATCTCCATCCCCTCCTTTCCAACACACTGCAATTCTAGATTGAAGACTGCACATTTATTTATTTATTTATTTTTTTGAGACGGA
SEQUENCE_INCLUDED_REGION=36,193
P3_COMMENT=CL is CTTGCTATATAGCCAAGTCGG
P3_COMMENT=CR is ATTGCAGTGTGTTGGAAAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.697841
PRIMER_LEFT_0_PENALTY=1.570745
PRIMER_RIGHT_0_PENALTY=0.127097
PRIMER_INTERNAL_0_PENALTY=1.032397
PRIMER_LEFT_0_SEQUENCE=CCCTTGATAATTTCATGCCA
PRIMER_RIGHT_0_SEQUENCE=TGCAGTGTGTTGGAAAGGAG
PRIMER_INTERNAL_0_SEQUENCE=TGACTTGGCTCTGTCTGTCC
PRIMER_LEFT_0=76,20
PRIMER_RIGHT_0=175,20
PRIMER_INTERNAL_0=117,20
PRIMER_LEFT_0_TM=58.429
PRIMER_RIGHT_0_TM=59.873
PRIMER_INTERNAL_0_TM=58.968
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=1.707283
PRIMER_LEFT_1_PENALTY=1.580186
PRIMER_RIGHT_1_PENALTY=0.127097
PRIMER_INTERNAL_1_PENALTY=1.032397
PRIMER_LEFT_1_SEQUENCE=CCCTTGATAATTTCATGCCAG
PRIMER_RIGHT_1_SEQUENCE=TGCAGTGTGTTGGAAAGGAG
PRIMER_INTERNAL_1_SEQUENCE=TGACTTGGCTCTGTCTGTCC
PRIMER_LEFT_1=76,21
PRIMER_RIGHT_1=175,20
PRIMER_INTERNAL_1=117,20
PRIMER_LEFT_1_TM=59.420
PRIMER_RIGHT_1_TM=59.873
PRIMER_INTERNAL_1_TM=58.968
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=1.982967
PRIMER_LEFT_2_PENALTY=1.570745
PRIMER_RIGHT_2_PENALTY=0.412222
PRIMER_INTERNAL_2_PENALTY=1.032397
PRIMER_LEFT_2_SEQUENCE=CCCTTGATAATTTCATGCCA
PRIMER_RIGHT_2_SEQUENCE=AAGGAGGGGATGGAGATGAG
PRIMER_INTERNAL_2_SEQUENCE=TGACTTGGCTCTGTCTGTCC
PRIMER_LEFT_2=76,20
PRIMER_RIGHT_2=161,20
PRIMER_INTERNAL_2=117,20
PRIMER_LEFT_2_TM=58.429
PRIMER_RIGHT_2_TM=60.412
PRIMER_INTERNAL_2_TM=58.968
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=1.992408
PRIMER_LEFT_3_PENALTY=1.580186
PRIMER_RIGHT_3_PENALTY=0.412222
PRIMER_INTERNAL_3_PENALTY=1.032397
PRIMER_LEFT_3_SEQUENCE=CCCTTGATAATTTCATGCCAG
PRIMER_RIGHT_3_SEQUENCE=AAGGAGGGGATGGAGATGAG
PRIMER_INTERNAL_3_SEQUENCE=TGACTTGGCTCTGTCTGTCC
PRIMER_LEFT_3=76,21
PRIMER_RIGHT_3=161,20
PRIMER_INTERNAL_3=117,20
PRIMER_LEFT_3_TM=59.420
PRIMER_RIGHT_3_TM=60.412
PRIMER_INTERNAL_3_TM=58.968
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=2.106319
PRIMER_LEFT_4_PENALTY=1.570745
PRIMER_RIGHT_4_PENALTY=0.535575
PRIMER_INTERNAL_4_PENALTY=1.032397
PRIMER_LEFT_4_SEQUENCE=CCCTTGATAATTTCATGCCA
PRIMER_RIGHT_4_SEQUENCE=CAGTGTGTTGGAAAGGAGGG
PRIMER_INTERNAL_4_SEQUENCE=TGACTTGGCTCTGTCTGTCC
PRIMER_LEFT_4=76,20
PRIMER_RIGHT_4=173,20
PRIMER_INTERNAL_4=117,20
PRIMER_LEFT_4_TM=58.429
PRIMER_RIGHT_4_TM=60.536
PRIMER_INTERNAL_4_TM=58.968
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH1075
SEQUENCE_TEMPLATE=TCTTCATGCTNCAGGTCGACTCTAGAGGATCCCCCTTGAATGAGGAGTNNCTTCTTATGGATTAGCAAAGCAAGTGGTTTCTTGAGATAGAATCTACTCCTGGTGAAGGCACTGTGNNNNTTGTTGAAATGNCAGTAAAAGATATAGAATATTCCATATTCTTAGATGATAAGGCAGTGGCAGGGTTTGAGAGGATTGACTCCAGTTGTGAAAGAAGTTCTACTGTGGGTAAAATGCTATCAAACAGNATCACAGGCTACAGAGAAATATTTTGTGAAAGGAAGAGTCAATCAATATGGCAAAGTTCATTGTTGTCTTATTTTAAGAAATTATCACAGCCATCTCTACCTTCAGCAACCAC
SEQUENCE_INCLUDED_REGION=34,327
P3_COMMENT=CL is TGGATTAGCAAAGCAAGTGG
P3_COMMENT=CR is TTGCTGAAGGTAGAGATGGC
P3_COMMENT=CM is TTCTTGAGATAGAATCTACTCCTGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.456778
PRIMER_LEFT_0_PENALTY=0.243525
PRIMER_RIGHT_0_PENALTY=2.213253
PRIMER_INTERNAL_0_PENALTY=6.677004
PRIMER_LEFT_0_SEQUENCE=ACTCCTGGTGAAGGCACTGT
PRIMER_RIGHT_0_SEQUENCE=TCCTCTCAAACCCTGCCAC
PRIMER_INTERNAL_0_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
PRIMER_LEFT_0=95,20
PRIMER_RIGHT_0=194,19
PRIMER_INTERNAL_0=151,25
PRIMER_LEFT_0_TM=59.756
PRIMER_RIGHT_0_TM=61.213
PRIMER_INTERNAL_0_TM=58.323
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_INTERNAL_0_GC_PERCENT=32.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=2.573257
PRIMER_LEFT_1_PENALTY=0.243525
PRIMER_RIGHT_1_PENALTY=2.329733
PRIMER_INTERNAL_1_PENALTY=6.677004
PRIMER_LEFT_1_SEQUENCE=ACTCCTGGTGAAGGCACTGT
PRIMER_RIGHT_1_SEQUENCE=TCCTCTCAAACCCTGCCA
PRIMER_INTERNAL_1_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
PRIMER_LEFT_1=95,20
PRIMER_RIGHT_1=194,18
PRIMER_INTERNAL_1=151,25
PRIMER_LEFT_1_TM=59.756
PRIMER_RIGHT_1_TM=60.330
PRIMER_INTERNAL_1_TM=58.323
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.556
PRIMER_INTERNAL_1_GC_PERCENT=32.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=3.063587
PRIMER_LEFT_2_PENALTY=0.243525
PRIMER_RIGHT_2_PENALTY=2.820062
PRIMER_INTERNAL_2_PENALTY=6.677004
PRIMER_LEFT_2_SEQUENCE=ACTCCTGGTGAAGGCACTGT
PRIMER_RIGHT_2_SEQUENCE=CCTCTCAAACCCTGCCAC
PRIMER_INTERNAL_2_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
PRIMER_LEFT_2=95,20
PRIMER_RIGHT_2=193,18
PRIMER_INTERNAL_2=151,25
PRIMER_LEFT_2_TM=59.756
PRIMER_RIGHT_2_TM=59.180
PRIMER_INTERNAL_2_TM=58.323
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_INTERNAL_2_GC_PERCENT=32.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=3.313830
PRIMER_LEFT_3_PENALTY=1.884424
PRIMER_RIGHT_3_PENALTY=1.429406
PRIMER_INTERNAL_3_PENALTY=6.677004
PRIMER_LEFT_3_SEQUENCE=CTCCTGGTGAAGGCACTGTG
PRIMER_RIGHT_3_SEQUENCE=ATCCTCTCAAACCCTGCCAC
PRIMER_INTERNAL_3_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
PRIMER_LEFT_3=96,20
PRIMER_RIGHT_3=195,20
PRIMER_INTERNAL_3=151,25
PRIMER_LEFT_3_TM=61.884
PRIMER_RIGHT_3_TM=61.429
PRIMER_INTERNAL_3_TM=58.323
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=32.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=3.316946
PRIMER_LEFT_4_PENALTY=1.887540
PRIMER_RIGHT_4_PENALTY=1.429406
PRIMER_INTERNAL_4_PENALTY=6.677004
PRIMER_LEFT_4_SEQUENCE=TCCTGGTGAAGGCACTGTG
PRIMER_RIGHT_4_SEQUENCE=ATCCTCTCAAACCCTGCCAC
PRIMER_INTERNAL_4_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA
PRIMER_LEFT_4=97,19
PRIMER_RIGHT_4=195,20
PRIMER_INTERNAL_4=151,25
PRIMER_LEFT_4_TM=60.888
PRIMER_RIGHT_4_TM=61.429
PRIMER_INTERNAL_4_TM=58.323
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH1076
SEQUENCE_TEMPLATE=AGGTGAACGTGTGGTTAAAACTAGGCAATTGGTTAAAAATCAATTTAAAAAACAGGCCTAGAAACAGTGACCACACCTCAAGCAATGATTATCCCTAGCACTCAGATTATGTTCTTGAAATACCATTTTCTGCTTTCAAAAGAAAGACATGAGGGCTTCTTGAAGAAATGGCTGATACCAAGCCTGCAGTGAAAAATGCACATGATGAGCCTGGAACATGTTGTCATATGAGTTAAAAAGGAAG
SEQUENCE_INCLUDED_REGION=0,244
P3_COMMENT=CL is TGAACGTGTGGTTAAAACTAGG
P3_COMMENT=CR is ACAACATGTTCCAGGCTCAT
P3_COMMENT=CM is TTAAAAATCAATTTAAAAAACAGGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.511601
PRIMER_LEFT_0_PENALTY=1.193666
PRIMER_RIGHT_0_PENALTY=0.317935
PRIMER_INTERNAL_0_PENALTY=0.248900
PRIMER_LEFT_0_SEQUENCE=GAGGGCTTCTTGAAGAAATGG
PRIMER_RIGHT_0_SEQUENCE=GACAACATGTTCCAGGCTCA
PRIMER_INTERNAL_0_SEQUENCE=CCAAGCCTGCAGTGAAAAAT
PRIMER_LEFT_0=150,21
PRIMER_RIGHT_0=224,20
PRIMER_INTERNAL_0=177,20
PRIMER_LEFT_0_TM=60.194
PRIMER_RIGHT_0_TM=59.682
PRIMER_INTERNAL_0_TM=60.249
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=1.511601
PRIMER_LEFT_1_PENALTY=1.193666
PRIMER_RIGHT_1_PENALTY=0.317935
PRIMER_INTERNAL_1_PENALTY=0.248900
PRIMER_LEFT_1_SEQUENCE=GAGGGCTTCTTGAAGAAATGG
PRIMER_RIGHT_1_SEQUENCE=TGACAACATGTTCCAGGCTC
PRIMER_INTERNAL_1_SEQUENCE=CCAAGCCTGCAGTGAAAAAT
PRIMER_LEFT_1=150,21
PRIMER_RIGHT_1=225,20
PRIMER_INTERNAL_1=177,20
PRIMER_LEFT_1_TM=60.194
PRIMER_RIGHT_1_TM=59.682
PRIMER_INTERNAL_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=1.652107
PRIMER_LEFT_2_PENALTY=1.580170
PRIMER_RIGHT_2_PENALTY=0.071937
PRIMER_INTERNAL_2_PENALTY=0.095771
PRIMER_LEFT_2_SEQUENCE=GACATGAGGGCTTCTTGAAGA
PRIMER_RIGHT_2_SEQUENCE=CATGTTCCAGGCTCATCATG
PRIMER_INTERNAL_2_SEQUENCE=AATGGCTGATACCAAGCCTG
PRIMER_LEFT_2=145,21
PRIMER_RIGHT_2=219,20
PRIMER_INTERNAL_2=166,20
PRIMER_LEFT_2_TM=59.420
PRIMER_RIGHT_2_TM=60.072
PRIMER_INTERNAL_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=1.769643
PRIMER_LEFT_3_PENALTY=1.697705
PRIMER_RIGHT_3_PENALTY=0.071937
PRIMER_INTERNAL_3_PENALTY=0.095771
PRIMER_LEFT_3_SEQUENCE=TCAAAAGAAAGACATGAGGGC
PRIMER_RIGHT_3_SEQUENCE=CATGTTCCAGGCTCATCATG
PRIMER_INTERNAL_3_SEQUENCE=AATGGCTGATACCAAGCCTG
PRIMER_LEFT_3=135,21
PRIMER_RIGHT_3=219,20
PRIMER_INTERNAL_3=166,20
PRIMER_LEFT_3_TM=59.302
PRIMER_RIGHT_3_TM=60.072
PRIMER_INTERNAL_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=1.879457
PRIMER_LEFT_4_PENALTY=1.561523
PRIMER_RIGHT_4_PENALTY=0.317935
PRIMER_INTERNAL_4_PENALTY=0.248900
PRIMER_LEFT_4_SEQUENCE=TGAGGGCTTCTTGAAGAAATG
PRIMER_RIGHT_4_SEQUENCE=GACAACATGTTCCAGGCTCA
PRIMER_INTERNAL_4_SEQUENCE=CCAAGCCTGCAGTGAAAAAT
PRIMER_LEFT_4=149,21
PRIMER_RIGHT_4=224,20
PRIMER_INTERNAL_4=177,20
PRIMER_LEFT_4_TM=59.438
PRIMER_RIGHT_4_TM=59.682
PRIMER_INTERNAL_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=9.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1080
SEQUENCE_TEMPLATE=CAAGGCACGTCTCAGGGTCCTGGGATCTGGGACAGGTAGGGACATTGCACCAGTCGAAGGGGAATTTTGAGGGTCTGGGGCCCTCCACTTCCGTCCCCCTCCAGCCTTACCCTCCACCTCTGGGTGCTTCATGAGCAGCAAGAAGCCATAGCGCAGGGTGGTGCTGACCGTCTCGGTGCCTGCAATGAAGAGGTTCAACGTGCTCATCATCAGGTTCTTCAAGTAGAACTCCGTNTTGGGGTTCTTCTCCTCCTGCAGGGAGAGGGGGCTTTAGG
SEQUENCE_INCLUDED_REGION=0,275
P3_COMMENT=CL is GACAGGTAGGGACATTGCAC
P3_COMMENT=CR is ACGGAGTTCTACTTGAAGAACC
P3_COMMENT=CM is AGTCGAAGGGGAATTTTGAG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.304879
PRIMER_LEFT_0_PENALTY=0.044122
PRIMER_RIGHT_0_PENALTY=0.260757
PRIMER_INTERNAL_0_PENALTY=2.394220
PRIMER_LEFT_0_SEQUENCE=CAGTCGAAGGGGAATTTTGA
PRIMER_RIGHT_0_SEQUENCE=CTCATGAAGCACCCAGAGGT
PRIMER_INTERNAL_0_SEQUENCE=CCTCCAGCCTTACCCTCC
PRIMER_LEFT_0=50,20
PRIMER_RIGHT_0=134,20
PRIMER_INTERNAL_0=97,18
PRIMER_LEFT_0_TM=60.044
PRIMER_RIGHT_0_TM=60.261
PRIMER_INTERNAL_0_TM=59.606
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=0.339625
PRIMER_LEFT_1_PENALTY=0.044122
PRIMER_RIGHT_1_PENALTY=0.295503
PRIMER_INTERNAL_1_PENALTY=0.639568
PRIMER_LEFT_1_SEQUENCE=CAGTCGAAGGGGAATTTTGA
PRIMER_RIGHT_1_SEQUENCE=TCTTGCTGCTCATGAAGCAC
PRIMER_INTERNAL_1_SEQUENCE=AGCCTTACCCTCCACCTCTG
PRIMER_LEFT_1=50,20
PRIMER_RIGHT_1=142,20
PRIMER_INTERNAL_1=102,20
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=60.296
PRIMER_INTERNAL_1_TM=60.640
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=0.373082
PRIMER_LEFT_2_PENALTY=0.010545
PRIMER_RIGHT_2_PENALTY=0.362536
PRIMER_INTERNAL_2_PENALTY=0.255861
PRIMER_LEFT_2_SEQUENCE=AGGTAGGGACATTGCACCAG
PRIMER_RIGHT_2_SEQUENCE=ACCCAGAGGTGGAGGGTAAG
PRIMER_INTERNAL_2_SEQUENCE=GAAGGGGAATTTTGAGGGTC
PRIMER_LEFT_2=33,20
PRIMER_RIGHT_2=124,20
PRIMER_INTERNAL_2=55,20
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=60.363
PRIMER_INTERNAL_2_TM=59.744
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=0.406659
PRIMER_LEFT_3_PENALTY=0.044122
PRIMER_RIGHT_3_PENALTY=0.362536
PRIMER_INTERNAL_3_PENALTY=4.432013
PRIMER_LEFT_3_SEQUENCE=CAGTCGAAGGGGAATTTTGA
PRIMER_RIGHT_3_SEQUENCE=ACCCAGAGGTGGAGGGTAAG
PRIMER_INTERNAL_3_SEQUENCE=ACTTCCGTCCCCCTCCAG
PRIMER_LEFT_3=50,20
PRIMER_RIGHT_3=124,20
PRIMER_INTERNAL_3=86,18
PRIMER_LEFT_3_TM=60.044
PRIMER_RIGHT_3_TM=60.363
PRIMER_INTERNAL_3_TM=62.432
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=66.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=0.420506
PRIMER_LEFT_4_PENALTY=0.260757
PRIMER_RIGHT_4_PENALTY=0.159750
PRIMER_INTERNAL_4_PENALTY=2.261603
PRIMER_LEFT_4_SEQUENCE=ACCTCTGGGTGCTTCATGAG
PRIMER_RIGHT_4_SEQUENCE=CGTTGAACCTCTTCATTGCA
PRIMER_INTERNAL_4_SEQUENCE=GGTGGTGCTGACCGTCTC
PRIMER_LEFT_4=115,20
PRIMER_RIGHT_4=199,20
PRIMER_INTERNAL_4=156,18
PRIMER_LEFT_4_TM=60.261
PRIMER_RIGHT_4_TM=59.840
PRIMER_INTERNAL_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH1082
SEQUENCE_TEMPLATE=AAACACTGTTATTCAATTTAATTTTGCTTGAAACCAATACACATGTATTTGAAATCATATTGACTGGGAATTTGGATCCTAGCAATATAAATTCCCCACATCACAAAGTACTTGAGGTGCTGAATGAACTAAAATAAAAGATAACTATTTATATTTATTTTNGTTCAATCATAATAATGTATTTAATTTATTATGTTGCAGCCCTAAAGAATTAAAGGGAG
SEQUENCE_INCLUDED_REGION=0,221
P3_COMMENT=CL is TAATTTTGCTTGAAACCAATACA
P3_COMMENT=CR is CCTTTAATTCTTTAGGGCTGC
P3_COMMENT=CM is TGTATTTGAAATCATATTGACTGGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.341381
PRIMER_LEFT_0_PENALTY=0.273480
PRIMER_RIGHT_0_PENALTY=4.067900
PRIMER_INTERNAL_0_PENALTY=2.636481
PRIMER_LEFT_0_SEQUENCE=TTGACTGGGAATTTGGATCC
PRIMER_RIGHT_0_SEQUENCE=TTTTAGTTCATTCAGCACCTCAA
PRIMER_INTERNAL_0_SEQUENCE=AAATTCCCCACATCACAAAG
PRIMER_LEFT_0=59,20
PRIMER_RIGHT_0=133,23
PRIMER_INTERNAL_0=88,20
PRIMER_LEFT_0_TM=59.727
PRIMER_RIGHT_0_TM=58.932
PRIMER_INTERNAL_0_TM=57.364
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=34.783
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.8000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=4.445401
PRIMER_LEFT_1_PENALTY=0.273480
PRIMER_RIGHT_1_PENALTY=4.171921
PRIMER_INTERNAL_1_PENALTY=2.636481
PRIMER_LEFT_1_SEQUENCE=TTGACTGGGAATTTGGATCC
PRIMER_RIGHT_1_SEQUENCE=TTTTAGTTCATTCAGCACCTCAAG
PRIMER_INTERNAL_1_SEQUENCE=AAATTCCCCACATCACAAAG
PRIMER_LEFT_1=59,20
PRIMER_RIGHT_1=133,24
PRIMER_INTERNAL_1=88,20
PRIMER_LEFT_1_TM=59.727
PRIMER_RIGHT_1_TM=59.828
PRIMER_INTERNAL_1_TM=57.364
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=37.500
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=4.700234
PRIMER_LEFT_2_PENALTY=0.273480
PRIMER_RIGHT_2_PENALTY=4.426754
PRIMER_INTERNAL_2_PENALTY=2.636481
PRIMER_LEFT_2_SEQUENCE=TTGACTGGGAATTTGGATCC
PRIMER_RIGHT_2_SEQUENCE=TTTTAGTTCATTCAGCACCTCA
PRIMER_INTERNAL_2_SEQUENCE=AAATTCCCCACATCACAAAG
PRIMER_LEFT_2=59,20
PRIMER_RIGHT_2=133,22
PRIMER_INTERNAL_2=88,20
PRIMER_LEFT_2_TM=59.727
PRIMER_RIGHT_2_TM=57.573
PRIMER_INTERNAL_2_TM=57.364
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=36.364
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=5.046100
PRIMER_LEFT_3_PENALTY=0.273480
PRIMER_RIGHT_3_PENALTY=4.772620
PRIMER_INTERNAL_3_PENALTY=2.636481
PRIMER_LEFT_3_SEQUENCE=TTGACTGGGAATTTGGATCC
PRIMER_RIGHT_3_SEQUENCE=ATTTTAGTTCATTCAGCACCTCAA
PRIMER_INTERNAL_3_SEQUENCE=AAATTCCCCACATCACAAAG
PRIMER_LEFT_3=59,20
PRIMER_RIGHT_3=134,24
PRIMER_INTERNAL_3=88,20
PRIMER_LEFT_3_TM=59.727
PRIMER_RIGHT_3_TM=59.227
PRIMER_INTERNAL_3_TM=57.364
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=5.077873
PRIMER_LEFT_4_PENALTY=1.009973
PRIMER_RIGHT_4_PENALTY=4.067900
PRIMER_INTERNAL_4_PENALTY=2.636481
PRIMER_LEFT_4_SEQUENCE=ATTGACTGGGAATTTGGATCC
PRIMER_RIGHT_4_SEQUENCE=TTTTAGTTCATTCAGCACCTCAA
PRIMER_INTERNAL_4_SEQUENCE=AAATTCCCCACATCACAAAG
PRIMER_LEFT_4=58,21
PRIMER_RIGHT_4=133,23
PRIMER_INTERNAL_4=88,20
PRIMER_LEFT_4_TM=60.010
PRIMER_RIGHT_4_TM=58.932
PRIMER_INTERNAL_4_TM=57.364
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1083
SEQUENCE_TEMPLATE=TTGTTCAAGGAGACATATACAAAGATGTATATTTTTGAGTTGTTAATTGTTACAAGGCTAAGAAAATCTTATTGTCAAACAGCAGGGGGATGAAAAAATACACTGTTGTTTGTTGGTCCAGCAGAATGCCTAAAATGAAAATGAATGAATTGGATTTGAATGTATCAACATAGCAAGTCTCAGAGATATAATGTTGTGGCTAAAAAAAAAAAAAGTTGAATATAGNGTATCAANCATTTAGGAGTTTATC
SEQUENCE_INCLUDED_REGION=0,250
P3_COMMENT=CL is GTTCAAGGAGACATATACAAAGATG
P3_COMMENT=CR is TATTCAACTTTTTTTTTTTTTAGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.390337
PRIMER_LEFT_0_PENALTY=1.376703
PRIMER_RIGHT_0_PENALTY=3.013634
PRIMER_INTERNAL_0_PENALTY=2.022362
PRIMER_LEFT_0_SEQUENCE=TTATTGTCAAACAGCAGGGG
PRIMER_RIGHT_0_SEQUENCE=CATTTTCATTTTAGGCATTCTGC
PRIMER_INTERNAL_0_SEQUENCE=CACTGTTGTTTGTTGGTCCA
PRIMER_LEFT_0=68,20
PRIMER_RIGHT_0=142,23
PRIMER_INTERNAL_0=100,20
PRIMER_LEFT_0_TM=58.623
PRIMER_RIGHT_0_TM=59.986
PRIMER_INTERNAL_0_TM=57.978
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=34.783
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=4.395781
PRIMER_LEFT_1_PENALTY=1.382147
PRIMER_RIGHT_1_PENALTY=3.013634
PRIMER_INTERNAL_1_PENALTY=2.022362
PRIMER_LEFT_1_SEQUENCE=CTTATTGTCAAACAGCAGGGG
PRIMER_RIGHT_1_SEQUENCE=CATTTTCATTTTAGGCATTCTGC
PRIMER_INTERNAL_1_SEQUENCE=CACTGTTGTTTGTTGGTCCA
PRIMER_LEFT_1=67,21
PRIMER_RIGHT_1=142,23
PRIMER_INTERNAL_1=100,20
PRIMER_LEFT_1_TM=59.618
PRIMER_RIGHT_1_TM=59.986
PRIMER_INTERNAL_1_TM=57.978
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=5.045595
PRIMER_LEFT_2_PENALTY=0.883387
PRIMER_RIGHT_2_PENALTY=4.162208
PRIMER_INTERNAL_2_PENALTY=0.213506
PRIMER_LEFT_2_SEQUENCE=TATTGTCAAACAGCAGGGGG
PRIMER_RIGHT_2_SEQUENCE=CAAATCCAATTCATTCATTTTCA
PRIMER_INTERNAL_2_SEQUENCE=GGTCCAGCAGAATGCCTAAA
PRIMER_LEFT_2=69,20
PRIMER_RIGHT_2=157,23
PRIMER_INTERNAL_2=114,20
PRIMER_LEFT_2_TM=60.883
PRIMER_RIGHT_2_TM=58.838
PRIMER_INTERNAL_2_TM=60.214
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=26.087
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=5.171308
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=4.957802
PRIMER_INTERNAL_3_PENALTY=4.162208
PRIMER_LEFT_3_SEQUENCE=GGTCCAGCAGAATGCCTAAA
PRIMER_RIGHT_3_SEQUENCE=TTTTTTTTTTTTTAGCCACAACA
PRIMER_INTERNAL_3_SEQUENCE=TGAAAATGAATGAATTGGATTTG
PRIMER_LEFT_3=114,20
PRIMER_RIGHT_3=213,23
PRIMER_INTERNAL_3=135,23
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=58.042
PRIMER_INTERNAL_3_TM=58.838
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=21.739
PRIMER_INTERNAL_3_GC_PERCENT=26.087
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=5.218852
PRIMER_LEFT_4_PENALTY=0.883387
PRIMER_RIGHT_4_PENALTY=4.335465
PRIMER_INTERNAL_4_PENALTY=0.277985
PRIMER_LEFT_4_SEQUENCE=TATTGTCAAACAGCAGGGGG
PRIMER_RIGHT_4_SEQUENCE=TTCATTCATTTTCATTTTAGGCA
PRIMER_INTERNAL_4_SEQUENCE=TGTTTGTTGGTCCAGCAGAA
PRIMER_LEFT_4=69,20
PRIMER_RIGHT_4=148,23
PRIMER_INTERNAL_4=106,20
PRIMER_LEFT_4_TM=60.883
PRIMER_RIGHT_4_TM=58.665
PRIMER_INTERNAL_4_TM=60.278
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=26.087
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH1084
SEQUENCE_TEMPLATE=GTGTGAGAGAGATAGAAACAGAGAGAAGAAGAAAAGAGGCAGATTGGAAGTGTGAAAAAAATGAAAGAAGAAGAAAAAAAGAGTCTAAATATTCAGAAATGTAAGTGCTGCCCTCAACTGTTCTTTACCCACTTAATTCTGCAATTTTGAAAACTAGATTGAATTCCTTTGCAAAACCCTTGCATCATGGATACCCGAGTTAAACCGTTAATTAAAAGACATTAAACATGGCCTGGTGGCAATCTCCACGNCTCCAAGTCAGGAGCAGGAAAGGAAAAACAGCAG
SEQUENCE_INCLUDED_REGION=0,285
P3_COMMENT=CL is AGAGGCAGATTGGAAGTGTG
P3_COMMENT=CR is CACCAGGCCATGTTTAATGT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.169834
PRIMER_LEFT_0_PENALTY=0.085951
PRIMER_RIGHT_0_PENALTY=0.083883
PRIMER_INTERNAL_0_PENALTY=3.325541
PRIMER_LEFT_0_SEQUENCE=AAGTGCTGCCCTCAACTGTT
PRIMER_RIGHT_0_SEQUENCE=GGGTTTTGCAAAGGAATTCA
PRIMER_INTERNAL_0_SEQUENCE=CCCACTTAATTCTGCAATTTTG
PRIMER_LEFT_0=102,20
PRIMER_RIGHT_0=178,20
PRIMER_INTERNAL_0=127,22
PRIMER_LEFT_0_TM=59.914
PRIMER_RIGHT_0_TM=59.916
PRIMER_INTERNAL_0_TM=58.674
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=0.200855
PRIMER_LEFT_1_PENALTY=0.183145
PRIMER_RIGHT_1_PENALTY=0.017711
PRIMER_INTERNAL_1_PENALTY=1.137415
PRIMER_LEFT_1_SEQUENCE=TGGATACCCGAGTTAAACCG
PRIMER_RIGHT_1_SEQUENCE=CTTTCCTGCTCCTGACTTGG
PRIMER_INTERNAL_1_SEQUENCE=CATTAAACATGGCCTGGTGG
PRIMER_LEFT_1=187,20
PRIMER_RIGHT_1=272,20
PRIMER_INTERNAL_1=219,20
PRIMER_LEFT_1_TM=59.817
PRIMER_RIGHT_1_TM=59.982
PRIMER_INTERNAL_1_TM=61.137
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=86
PRIMER_PAIR_2_PENALTY=0.200855
PRIMER_LEFT_2_PENALTY=0.183145
PRIMER_RIGHT_2_PENALTY=0.017711
PRIMER_INTERNAL_2_PENALTY=1.137415
PRIMER_LEFT_2_SEQUENCE=TGGATACCCGAGTTAAACCG
PRIMER_RIGHT_2_SEQUENCE=CCTTTCCTGCTCCTGACTTG
PRIMER_INTERNAL_2_SEQUENCE=CATTAAACATGGCCTGGTGG
PRIMER_LEFT_2=187,20
PRIMER_RIGHT_2=273,20
PRIMER_INTERNAL_2=219,20
PRIMER_LEFT_2_TM=59.817
PRIMER_RIGHT_2_TM=59.982
PRIMER_INTERNAL_2_TM=61.137
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=0.238063
PRIMER_LEFT_3_PENALTY=0.220352
PRIMER_RIGHT_3_PENALTY=0.017711
PRIMER_INTERNAL_3_PENALTY=1.137415
PRIMER_LEFT_3_SEQUENCE=GCATCATGGATACCCGAGTT
PRIMER_RIGHT_3_SEQUENCE=CTTTCCTGCTCCTGACTTGG
PRIMER_INTERNAL_3_SEQUENCE=CATTAAACATGGCCTGGTGG
PRIMER_LEFT_3=181,20
PRIMER_RIGHT_3=272,20
PRIMER_INTERNAL_3=219,20
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=59.982
PRIMER_INTERNAL_3_TM=61.137
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=0.238063
PRIMER_LEFT_4_PENALTY=0.220352
PRIMER_RIGHT_4_PENALTY=0.017711
PRIMER_INTERNAL_4_PENALTY=1.137415
PRIMER_LEFT_4_SEQUENCE=GCATCATGGATACCCGAGTT
PRIMER_RIGHT_4_SEQUENCE=CCTTTCCTGCTCCTGACTTG
PRIMER_INTERNAL_4_SEQUENCE=CATTAAACATGGCCTGGTGG
PRIMER_LEFT_4=181,20
PRIMER_RIGHT_4=273,20
PRIMER_INTERNAL_4=219,20
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=59.982
PRIMER_INTERNAL_4_TM=61.137
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH1091
SEQUENCE_TEMPLATE=GGGGATGGAGAACTGCCCTCCTGACTCGCCCTGCCCAGGATCTGAAGTTGAAAGAAACAGAAAATCTTTAAACATGATTCTTATTTTGGTTGTGTGTTGAATCTGTGCCTTCCCTCTGAAAAACTCAGGGTTGCCCCAGGTAGGATTTTCCCCAGAACACCAGGGAGAGCACAAGATCAGGTTCCCAGTGCCAGAATGATGGTCAGGGAGGCATGTAACTGGTCAGACAAGGGCATCTGCCAGTGTAAACAAGTTTCTCATGAACT
SEQUENCE_INCLUDED_REGION=0,266
P3_COMMENT=CL is CCCAGGATCTGAAGTTGAAA
P3_COMMENT=CR is ATGAGAAACTTGTTTACACTGGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.179195
PRIMER_LEFT_0_PENALTY=0.168650
PRIMER_RIGHT_0_PENALTY=0.010545
PRIMER_INTERNAL_0_PENALTY=0.066262
PRIMER_LEFT_0_SEQUENCE=AGGATTTTCCCCAGAACACC
PRIMER_RIGHT_0_SEQUENCE=ACCAGTTACATGCCTCCCTG
PRIMER_INTERNAL_0_SEQUENCE=GTTCCCAGTGCCAGAATGAT
PRIMER_LEFT_0=141,20
PRIMER_RIGHT_0=222,20
PRIMER_INTERNAL_0=180,20
PRIMER_LEFT_0_TM=60.169
PRIMER_RIGHT_0_TM=59.989
PRIMER_INTERNAL_0_TM=59.934
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=0.197494
PRIMER_LEFT_1_PENALTY=0.186949
PRIMER_RIGHT_1_PENALTY=0.010545
PRIMER_INTERNAL_1_PENALTY=0.066262
PRIMER_LEFT_1_SEQUENCE=GTTGCCCCAGGTAGGATTTT
PRIMER_RIGHT_1_SEQUENCE=ACCAGTTACATGCCTCCCTG
PRIMER_INTERNAL_1_SEQUENCE=GTTCCCAGTGCCAGAATGAT
PRIMER_LEFT_1=129,20
PRIMER_RIGHT_1=222,20
PRIMER_INTERNAL_1=180,20
PRIMER_LEFT_1_TM=60.187
PRIMER_RIGHT_1_TM=59.989
PRIMER_INTERNAL_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=0.263165
PRIMER_LEFT_2_PENALTY=0.196903
PRIMER_RIGHT_2_PENALTY=0.066262
PRIMER_INTERNAL_2_PENALTY=0.168650
PRIMER_LEFT_2_SEQUENCE=ATCTGTGCCTTCCCTCTGAA
PRIMER_RIGHT_2_SEQUENCE=ATCATTCTGGCACTGGGAAC
PRIMER_INTERNAL_2_SEQUENCE=AGGATTTTCCCCAGAACACC
PRIMER_LEFT_2=100,20
PRIMER_RIGHT_2=199,20
PRIMER_INTERNAL_2=141,20
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=59.934
PRIMER_INTERNAL_2_TM=60.169
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=0.288459
PRIMER_LEFT_3_PENALTY=0.168650
PRIMER_RIGHT_3_PENALTY=0.119809
PRIMER_INTERNAL_3_PENALTY=0.066262
PRIMER_LEFT_3_SEQUENCE=AGGATTTTCCCCAGAACACC
PRIMER_RIGHT_3_SEQUENCE=GATGCCCTTGTCTGACCAGT
PRIMER_INTERNAL_3_SEQUENCE=GTTCCCAGTGCCAGAATGAT
PRIMER_LEFT_3=141,20
PRIMER_RIGHT_3=236,20
PRIMER_INTERNAL_3=180,20
PRIMER_LEFT_3_TM=60.169
PRIMER_RIGHT_3_TM=60.120
PRIMER_INTERNAL_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=0.355066
PRIMER_LEFT_4_PENALTY=0.288804
PRIMER_RIGHT_4_PENALTY=0.066262
PRIMER_INTERNAL_4_PENALTY=0.168650
PRIMER_LEFT_4_SEQUENCE=CTGAAAAACTCAGGGTTGCC
PRIMER_RIGHT_4_SEQUENCE=ATCATTCTGGCACTGGGAAC
PRIMER_INTERNAL_4_SEQUENCE=AGGATTTTCCCCAGAACACC
PRIMER_LEFT_4=115,20
PRIMER_RIGHT_4=199,20
PRIMER_INTERNAL_4=141,20
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=59.934
PRIMER_INTERNAL_4_TM=60.169
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH1093
SEQUENCE_TEMPLATE=CAGGCTTCTCCATTTTCATGCCTACTTCTGCCTCCTGCTAAGTTGCATCAGTTTCAAACACAAAGGTTAAGATGTGTTACCTGGGTTAAGATGTCTTAGCCAGGGGTCAATCCATGACTATTACACTGTGGGGAAGTCTAGGTGATACTAGAGGGNTCAGGGTGCCTGGGTGACAGGTGATCACTTAGAGGGNTCCTGACAGTGCTTATTTACCANCTGGTACAGAAGTTTTTCAGTATCTTTAAACTGGTATGACTGTCCAGTGCATATTGGCTGACTATCAGCCTGA
SEQUENCE_INCLUDED_REGION=0,289
P3_COMMENT=CL is TTTTCATGCCTACTTCTGCC
P3_COMMENT=CR is CAGGCTGATAGTCAGCCAAT
P3_COMMENT=CM is CCTGCTAAGTTGCATCAGTTT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.088326
PRIMER_LEFT_0_PENALTY=0.015375
PRIMER_RIGHT_0_PENALTY=0.072951
PRIMER_INTERNAL_0_PENALTY=4.280778
PRIMER_LEFT_0_SEQUENCE=GCCTCCTGCTAAGTTGCATC
PRIMER_RIGHT_0_SEQUENCE=TGACCCCTGGCTAAGACATC
PRIMER_INTERNAL_0_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG
PRIMER_LEFT_0=29,20
PRIMER_RIGHT_0=108,20
PRIMER_INTERNAL_0=61,24
PRIMER_LEFT_0_TM=59.985
PRIMER_RIGHT_0_TM=60.073
PRIMER_INTERNAL_0_TM=59.719
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=0.434718
PRIMER_LEFT_1_PENALTY=0.361767
PRIMER_RIGHT_1_PENALTY=0.072951
PRIMER_INTERNAL_1_PENALTY=2.345718
PRIMER_LEFT_1_SEQUENCE=TCATGCCTACTTCTGCCTCC
PRIMER_RIGHT_1_SEQUENCE=TGACCCCTGGCTAAGACATC
PRIMER_INTERNAL_1_SEQUENCE=GCATCAGTTTCAAACACAAAGG
PRIMER_LEFT_1=15,20
PRIMER_RIGHT_1=108,20
PRIMER_INTERNAL_1=44,22
PRIMER_LEFT_1_TM=60.362
PRIMER_RIGHT_1_TM=60.073
PRIMER_INTERNAL_1_TM=59.654
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=0.465204
PRIMER_LEFT_2_PENALTY=0.015375
PRIMER_RIGHT_2_PENALTY=0.449829
PRIMER_INTERNAL_2_PENALTY=4.280778
PRIMER_LEFT_2_SEQUENCE=GCCTCCTGCTAAGTTGCATC
PRIMER_RIGHT_2_SEQUENCE=TTGACCCCTGGCTAAGACAT
PRIMER_INTERNAL_2_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG
PRIMER_LEFT_2=29,20
PRIMER_RIGHT_2=109,20
PRIMER_INTERNAL_2=61,24
PRIMER_LEFT_2_TM=59.985
PRIMER_RIGHT_2_TM=59.550
PRIMER_INTERNAL_2_TM=59.719
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=0.465204
PRIMER_LEFT_3_PENALTY=0.015375
PRIMER_RIGHT_3_PENALTY=0.449829
PRIMER_INTERNAL_3_PENALTY=4.280778
PRIMER_LEFT_3_SEQUENCE=GCCTCCTGCTAAGTTGCATC
PRIMER_RIGHT_3_SEQUENCE=ATTGACCCCTGGCTAAGACA
PRIMER_INTERNAL_3_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG
PRIMER_LEFT_3=29,20
PRIMER_RIGHT_3=110,20
PRIMER_INTERNAL_3=61,24
PRIMER_LEFT_3_TM=59.985
PRIMER_RIGHT_3_TM=59.550
PRIMER_INTERNAL_3_TM=59.719
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=0.619362
PRIMER_LEFT_4_PENALTY=0.546410
PRIMER_RIGHT_4_PENALTY=0.072951
PRIMER_INTERNAL_4_PENALTY=2.345718
PRIMER_LEFT_4_SEQUENCE=ATGCCTACTTCTGCCTCCTG
PRIMER_RIGHT_4_SEQUENCE=TGACCCCTGGCTAAGACATC
PRIMER_INTERNAL_4_SEQUENCE=GCATCAGTTTCAAACACAAAGG
PRIMER_LEFT_4=17,20
PRIMER_RIGHT_4=108,20
PRIMER_INTERNAL_4=44,22
PRIMER_LEFT_4_TM=59.454
PRIMER_RIGHT_4_TM=60.073
PRIMER_INTERNAL_4_TM=59.654
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH1095
SEQUENCE_TEMPLATE=TNNTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAATGGAGATTAATTTCTTTAGTAGGAAATGAGGAGCCTTTCATTCATTCAACACATATTTATTGAGTGCTTTCAGTGTGCCAACATCATTCTAAATGATTGGGATACATGAGTNGGAGTNAGTNAAGTGGACAATGGCAGCATTTTAGAAAAGGATTAAGGTAATCAACACTACTTTTTNAGAACAATGNGTCTGTGCCTTAAAAGTGTTTTGTGTGTGTGTGTGTGTTTAGGGGAGATGATCAGGACGATGGGGAGGCGCTATCAGTCAATGTATTGTTTGGGTGAGTGGTGATAAGTGCCTAATACACAAAGCGA
SEQUENCE_INCLUDED_REGION=36,323
P3_COMMENT=CL is TTTCTTTAGTAGGAAATGAGGAGC
P3_COMMENT=CR is TTTGTGTATTAGGCACTTATCACC
P3_COMMENT=CM is CATTCAACACATATTTATTGAGTGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.105706
PRIMER_LEFT_0_PENALTY=0.032351
PRIMER_RIGHT_0_PENALTY=0.073355
PRIMER_INTERNAL_0_PENALTY=5.835450
PRIMER_LEFT_0_SEQUENCE=GAGTGCTTTCAGTGTGCCAA
PRIMER_RIGHT_0_SEQUENCE=TAAAATGCTGCCATTGTCCA
PRIMER_INTERNAL_0_SEQUENCE=CATTCTAAATGATTGGGATACATGA
PRIMER_LEFT_0=105,20
PRIMER_RIGHT_0=188,20
PRIMER_INTERNAL_0=128,25
PRIMER_LEFT_0_TM=60.032
PRIMER_RIGHT_0_TM=60.073
PRIMER_INTERNAL_0_TM=59.165
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=32.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=0.105706
PRIMER_LEFT_1_PENALTY=0.032351
PRIMER_RIGHT_1_PENALTY=0.073355
PRIMER_INTERNAL_1_PENALTY=5.491396
PRIMER_LEFT_1_SEQUENCE=TTGAGTGCTTTCAGTGTGCC
PRIMER_RIGHT_1_SEQUENCE=TAAAATGCTGCCATTGTCCA
PRIMER_INTERNAL_1_SEQUENCE=AACATCATTCTAAATGATTGGGA
PRIMER_LEFT_1=103,20
PRIMER_RIGHT_1=188,20
PRIMER_INTERNAL_1=123,23
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.073
PRIMER_INTERNAL_1_TM=57.509
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=30.435
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=86
PRIMER_PAIR_2_PENALTY=0.383297
PRIMER_LEFT_2_PENALTY=0.309943
PRIMER_RIGHT_2_PENALTY=0.073355
PRIMER_INTERNAL_2_PENALTY=5.835450
PRIMER_LEFT_2_SEQUENCE=TGCTTTCAGTGTGCCAACAT
PRIMER_RIGHT_2_SEQUENCE=TAAAATGCTGCCATTGTCCA
PRIMER_INTERNAL_2_SEQUENCE=CATTCTAAATGATTGGGATACATGA
PRIMER_LEFT_2=108,20
PRIMER_RIGHT_2=188,20
PRIMER_INTERNAL_2=128,25
PRIMER_LEFT_2_TM=60.310
PRIMER_RIGHT_2_TM=60.073
PRIMER_INTERNAL_2_TM=59.165
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=32.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=0.558269
PRIMER_LEFT_3_PENALTY=0.032351
PRIMER_RIGHT_3_PENALTY=0.525918
PRIMER_INTERNAL_3_PENALTY=5.835450
PRIMER_LEFT_3_SEQUENCE=GAGTGCTTTCAGTGTGCCAA
PRIMER_RIGHT_3_SEQUENCE=AATGCTGCCATTGTCCACTT
PRIMER_INTERNAL_3_SEQUENCE=CATTCTAAATGATTGGGATACATGA
PRIMER_LEFT_3=105,20
PRIMER_RIGHT_3=185,20
PRIMER_INTERNAL_3=128,25
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.526
PRIMER_INTERNAL_3_TM=59.165
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=32.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=0.558269
PRIMER_LEFT_4_PENALTY=0.032351
PRIMER_RIGHT_4_PENALTY=0.525918
PRIMER_INTERNAL_4_PENALTY=5.835450
PRIMER_LEFT_4_SEQUENCE=GAGTGCTTTCAGTGTGCCAA
PRIMER_RIGHT_4_SEQUENCE=AAATGCTGCCATTGTCCACT
PRIMER_INTERNAL_4_SEQUENCE=CATTCTAAATGATTGGGATACATGA
PRIMER_LEFT_4=105,20
PRIMER_RIGHT_4=186,20
PRIMER_INTERNAL_4=128,25
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=60.526
PRIMER_INTERNAL_4_TM=59.165
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH1096
SEQUENCE_TEMPLATE=ACTCCCAAATCCAGAGATTTGTGTGTGCAAATGTGTATGTNTNAACGTTCAAAATTGAAGACTGTCTAACTCAGCGACCCTCAATCTTGACTGAGCATCAGGGTCATCTAGAG
SEQUENCE_INCLUDED_REGION=0,113
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.617746
PRIMER_LEFT_0_PENALTY=1.215635
PRIMER_RIGHT_0_PENALTY=0.402111
PRIMER_INTERNAL_0_PENALTY=1.404189
PRIMER_LEFT_0_SEQUENCE=TCCAGAGATTTGTGTGTGCA
PRIMER_RIGHT_0_SEQUENCE=TGACCCTGATGCTCAGTCAA
PRIMER_INTERNAL_0_SEQUENCE=TCTAACTCAGCGACCCTCAA
PRIMER_LEFT_0=9,20
PRIMER_RIGHT_0=105,20
PRIMER_INTERNAL_0=64,20
PRIMER_LEFT_0_TM=58.784
PRIMER_RIGHT_0_TM=60.402
PRIMER_INTERNAL_0_TM=58.596
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=1.653373
PRIMER_LEFT_1_PENALTY=1.251261
PRIMER_RIGHT_1_PENALTY=0.402111
PRIMER_INTERNAL_1_PENALTY=1.404189
PRIMER_LEFT_1_SEQUENCE=CCAGAGATTTGTGTGTGCAAA
PRIMER_RIGHT_1_SEQUENCE=TGACCCTGATGCTCAGTCAA
PRIMER_INTERNAL_1_SEQUENCE=TCTAACTCAGCGACCCTCAA
PRIMER_LEFT_1=10,21
PRIMER_RIGHT_1=105,20
PRIMER_INTERNAL_1=64,20
PRIMER_LEFT_1_TM=59.749
PRIMER_RIGHT_1_TM=60.402
PRIMER_INTERNAL_1_TM=58.596
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=1.696063
PRIMER_LEFT_2_PENALTY=1.293952
PRIMER_RIGHT_2_PENALTY=0.402111
PRIMER_INTERNAL_2_PENALTY=1.404189
PRIMER_LEFT_2_SEQUENCE=TCCAGAGATTTGTGTGTGCAA
PRIMER_RIGHT_2_SEQUENCE=TGACCCTGATGCTCAGTCAA
PRIMER_INTERNAL_2_SEQUENCE=TCTAACTCAGCGACCCTCAA
PRIMER_LEFT_2=9,21
PRIMER_RIGHT_2=105,20
PRIMER_INTERNAL_2=64,20
PRIMER_LEFT_2_TM=60.294
PRIMER_RIGHT_2_TM=60.402
PRIMER_INTERNAL_2_TM=58.596
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=2.004027
PRIMER_LEFT_3_PENALTY=1.215635
PRIMER_RIGHT_3_PENALTY=0.788392
PRIMER_INTERNAL_3_PENALTY=1.105717
PRIMER_LEFT_3_SEQUENCE=TCCAGAGATTTGTGTGTGCA
PRIMER_RIGHT_3_SEQUENCE=ATGACCCTGATGCTCAGTCA
PRIMER_INTERNAL_3_SEQUENCE=AACTCAGCGACCCTCAATCT
PRIMER_LEFT_3=9,20
PRIMER_RIGHT_3=106,20
PRIMER_INTERNAL_3=67,20
PRIMER_LEFT_3_TM=58.784
PRIMER_RIGHT_3_TM=59.212
PRIMER_INTERNAL_3_TM=58.894
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=2.016826
PRIMER_LEFT_4_PENALTY=1.215635
PRIMER_RIGHT_4_PENALTY=0.801192
PRIMER_INTERNAL_4_PENALTY=5.525458
PRIMER_LEFT_4_SEQUENCE=TCCAGAGATTTGTGTGTGCA
PRIMER_RIGHT_4_SEQUENCE=GTCAAGATTGAGGGTCGCTG
PRIMER_INTERNAL_4_SEQUENCE=AACGTTCAAAATTGAAGACTGTC
PRIMER_LEFT_4=9,20
PRIMER_RIGHT_4=90,20
PRIMER_INTERNAL_4=43,23
PRIMER_LEFT_4_TM=58.784
PRIMER_RIGHT_4_TM=60.801
PRIMER_INTERNAL_4_TM=57.475
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH1103
SEQUENCE_TEMPLATE=TCTGCATCCATCTATTTTTCAGATAAAACCATGGGAAGCATTTAACGTNCTAGTAAGTNTAAACAGTAAAATTTCCTTCCTCTGCTTTTCTCTTCTAATAACAAACAATAACTTAAACAGTTACACAAATACATATTCTTACAATAAAAATAGTTTATAATAACACTGTCCCAATAAAGACCACTATTAACTCTTACATCAAAAGGGAGTGGAGAAGAAAAATTTATATTAATAACTCCTTATCTCCCCAACTCTCCACCTCCCCTCAAAAAAAACCTCTGTGGAAAATTAATGTAATTAAG
SEQUENCE_INCLUDED_REGION=0,302
P3_COMMENT=CL is TCTGCATCCATCTATTTTTCA
P3_COMMENT=CR is TAATTACATTAATTTTCCACAGAGG
P3_COMMENT=CM is ATAAAACCATGGGAAGCATTT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.671902
PRIMER_LEFT_0_PENALTY=2.105078
PRIMER_RIGHT_0_PENALTY=0.566824
PRIMER_INTERNAL_0_PENALTY=1.440473
PRIMER_LEFT_0_SEQUENCE=TCAAAAGGGAGTGGAGAAGAA
PRIMER_RIGHT_0_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
PRIMER_INTERNAL_0_SEQUENCE=CCTTATCTCCCCAACTCTCCA
PRIMER_LEFT_0=198,21
PRIMER_RIGHT_0=277,20
PRIMER_INTERNAL_0=237,21
PRIMER_LEFT_0_TM=58.895
PRIMER_RIGHT_0_TM=59.433
PRIMER_INTERNAL_0_TM=60.440
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=2.780399
PRIMER_LEFT_1_PENALTY=2.213575
PRIMER_RIGHT_1_PENALTY=0.566824
PRIMER_INTERNAL_1_PENALTY=1.440473
PRIMER_LEFT_1_SEQUENCE=TCAAAAGGGAGTGGAGAAGAAA
PRIMER_RIGHT_1_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
PRIMER_INTERNAL_1_SEQUENCE=CCTTATCTCCCCAACTCTCCA
PRIMER_LEFT_1=198,22
PRIMER_RIGHT_1=277,20
PRIMER_INTERNAL_1=237,21
PRIMER_LEFT_1_TM=60.214
PRIMER_RIGHT_1_TM=59.433
PRIMER_INTERNAL_1_TM=60.440
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=2.791146
PRIMER_LEFT_2_PENALTY=2.224322
PRIMER_RIGHT_2_PENALTY=0.566824
PRIMER_INTERNAL_2_PENALTY=1.440473
PRIMER_LEFT_2_SEQUENCE=CATCAAAAGGGAGTGGAGAAG
PRIMER_RIGHT_2_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
PRIMER_INTERNAL_2_SEQUENCE=CCTTATCTCCCCAACTCTCCA
PRIMER_LEFT_2=196,21
PRIMER_RIGHT_2=277,20
PRIMER_INTERNAL_2=237,21
PRIMER_LEFT_2_TM=58.776
PRIMER_RIGHT_2_TM=59.433
PRIMER_INTERNAL_2_TM=60.440
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=2.801991
PRIMER_LEFT_3_PENALTY=2.105078
PRIMER_RIGHT_3_PENALTY=0.696913
PRIMER_INTERNAL_3_PENALTY=1.478252
PRIMER_LEFT_3_SEQUENCE=TCAAAAGGGAGTGGAGAAGAA
PRIMER_RIGHT_3_SEQUENCE=GGTTTTTTTTGAGGGGAGGT
PRIMER_INTERNAL_3_SEQUENCE=TCCTTATCTCCCCAACTCTCC
PRIMER_LEFT_3=198,21
PRIMER_RIGHT_3=276,20
PRIMER_INTERNAL_3=236,21
PRIMER_LEFT_3_TM=58.895
PRIMER_RIGHT_3_TM=59.303
PRIMER_INTERNAL_3_TM=59.522
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=2.831268
PRIMER_LEFT_4_PENALTY=2.264444
PRIMER_RIGHT_4_PENALTY=0.566824
PRIMER_INTERNAL_4_PENALTY=1.440473
PRIMER_LEFT_4_SEQUENCE=CAAAAGGGAGTGGAGAAGAAAA
PRIMER_RIGHT_4_SEQUENCE=AGGTTTTTTTTGAGGGGAGG
PRIMER_INTERNAL_4_SEQUENCE=CCTTATCTCCCCAACTCTCCA
PRIMER_LEFT_4=199,22
PRIMER_RIGHT_4=277,20
PRIMER_INTERNAL_4=237,21
PRIMER_LEFT_4_TM=59.736
PRIMER_RIGHT_4_TM=59.433
PRIMER_INTERNAL_4_TM=60.440
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH1109
SEQUENCE_TEMPLATE=NTTTTNNNATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGACCCTAGAGTGTCTTCTGAATAAACCATACCAAGAAGAAAAAATCTATGATGACACAGTCTTGGTTTCTCTACTGGGACATTGCTAAGATGTGGGTTTCCAATAAAAGGATATTGCTACCTTCAATATGAGGCCTAGTTAACTGACCAACCCCACTGAGTAAGCATCTTTTGACATTTCACTGATTTCAGGACTAGAAAAAATGGAAAGTTCACAGTCCCTAGAAATCTTCTTCTAGTCTCAGTGTAAATCTTATATATTTGATGGGATGTTTCAAACTTCTCCCATAAATGAA
SEQUENCE_INCLUDED_REGION=38,300
P3_COMMENT=CL is GAGTGTCTTCTGAATAAACCATACC
P3_COMMENT=CR is TTCTAGGGACTGTGAACTTTCC
P3_COMMENT=CM is AAAATCTATGATGACACAGTCTTGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.879014
PRIMER_LEFT_0_PENALTY=0.877035
PRIMER_RIGHT_0_PENALTY=0.001978
PRIMER_INTERNAL_0_PENALTY=1.804465
PRIMER_LEFT_0_SEQUENCE=TGCTAAGATGTGGGTTTCCA
PRIMER_RIGHT_0_SEQUENCE=CTCAGTGGGGTTGGTCAGTT
PRIMER_INTERNAL_0_SEQUENCE=TGCTACCTTCAATATGAGGCC
PRIMER_LEFT_0=125,20
PRIMER_RIGHT_0=202,20
PRIMER_INTERNAL_0=157,21
PRIMER_LEFT_0_TM=59.123
PRIMER_RIGHT_0_TM=60.002
PRIMER_INTERNAL_0_TM=59.196
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=1.426314
PRIMER_LEFT_1_PENALTY=0.877035
PRIMER_RIGHT_1_PENALTY=0.549279
PRIMER_INTERNAL_1_PENALTY=1.804465
PRIMER_LEFT_1_SEQUENCE=TGCTAAGATGTGGGTTTCCA
PRIMER_RIGHT_1_SEQUENCE=ACTCAGTGGGGTTGGTCAGT
PRIMER_INTERNAL_1_SEQUENCE=TGCTACCTTCAATATGAGGCC
PRIMER_LEFT_1=125,20
PRIMER_RIGHT_1=203,20
PRIMER_INTERNAL_1=157,21
PRIMER_LEFT_1_TM=59.123
PRIMER_RIGHT_1_TM=59.451
PRIMER_INTERNAL_1_TM=59.196
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=1.489075
PRIMER_LEFT_2_PENALTY=1.487097
PRIMER_RIGHT_2_PENALTY=0.001978
PRIMER_INTERNAL_2_PENALTY=1.804465
PRIMER_LEFT_2_SEQUENCE=TGCTAAGATGTGGGTTTCCAA
PRIMER_RIGHT_2_SEQUENCE=CTCAGTGGGGTTGGTCAGTT
PRIMER_INTERNAL_2_SEQUENCE=TGCTACCTTCAATATGAGGCC
PRIMER_LEFT_2=125,21
PRIMER_RIGHT_2=202,20
PRIMER_INTERNAL_2=157,21
PRIMER_LEFT_2_TM=60.487
PRIMER_RIGHT_2_TM=60.002
PRIMER_INTERNAL_2_TM=59.196
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=1.489075
PRIMER_LEFT_3_PENALTY=1.487097
PRIMER_RIGHT_3_PENALTY=0.001978
PRIMER_INTERNAL_3_PENALTY=1.804465
PRIMER_LEFT_3_SEQUENCE=TTGCTAAGATGTGGGTTTCCA
PRIMER_RIGHT_3_SEQUENCE=CTCAGTGGGGTTGGTCAGTT
PRIMER_INTERNAL_3_SEQUENCE=TGCTACCTTCAATATGAGGCC
PRIMER_LEFT_3=124,21
PRIMER_RIGHT_3=202,20
PRIMER_INTERNAL_3=157,21
PRIMER_LEFT_3_TM=60.487
PRIMER_RIGHT_3_TM=60.002
PRIMER_INTERNAL_3_TM=59.196
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=1.811911
PRIMER_LEFT_4_PENALTY=0.877035
PRIMER_RIGHT_4_PENALTY=0.934876
PRIMER_INTERNAL_4_PENALTY=1.804465
PRIMER_LEFT_4_SEQUENCE=TGCTAAGATGTGGGTTTCCA
PRIMER_RIGHT_4_SEQUENCE=GCTTACTCAGTGGGGTTGGT
PRIMER_INTERNAL_4_SEQUENCE=TGCTACCTTCAATATGAGGCC
PRIMER_LEFT_4=125,20
PRIMER_RIGHT_4=207,20
PRIMER_INTERNAL_4=157,21
PRIMER_LEFT_4_TM=59.123
PRIMER_RIGHT_4_TM=59.065
PRIMER_INTERNAL_4_TM=59.196
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=83
=
SEQUENCE_ID=MH1113
SEQUENCE_TEMPLATE=TCACCTTTTCAGGCCCTGGCAGAAGGGTGAAGGCAAAGGCCCCAGACACAGGNNTAGAAGAGATGGNATCTGGNNGCCAATGCAAGACCCACCTTTTACAGATGGGAG
SEQUENCE_INCLUDED_REGION=0,108
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.273937
PRIMER_LEFT_0_PENALTY=2.244684
PRIMER_RIGHT_0_PENALTY=0.029253
PRIMER_INTERNAL_0_PENALTY=1.730588
PRIMER_LEFT_0_SEQUENCE=TCACCTTTTCAGGCCCTG
PRIMER_RIGHT_0_SEQUENCE=AAAAGGTGGGTCTTGCATTG
PRIMER_INTERNAL_0_SEQUENCE=AGAAGGGTGAAGGCAAAGG
PRIMER_LEFT_0=0,18
PRIMER_RIGHT_0=96,20
PRIMER_INTERNAL_0=20,19
PRIMER_LEFT_0_TM=59.755
PRIMER_RIGHT_0_TM=59.971
PRIMER_INTERNAL_0_TM=59.269
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=52.632
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=2.693946
PRIMER_LEFT_1_PENALTY=2.664693
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_INTERNAL_1_PENALTY=2.236832
PRIMER_LEFT_1_SEQUENCE=CTGGCAGAAGGGTGAAGG
PRIMER_RIGHT_1_SEQUENCE=AAAAGGTGGGTCTTGCATTG
PRIMER_INTERNAL_1_SEQUENCE=CAAAGGCCCCAGACACAG
PRIMER_LEFT_1=15,18
PRIMER_RIGHT_1=96,20
PRIMER_INTERNAL_1=33,18
PRIMER_LEFT_1_TM=59.335
PRIMER_RIGHT_1_TM=59.971
PRIMER_INTERNAL_1_TM=60.237
PRIMER_LEFT_1_GC_PERCENT=61.111
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=2.693946
PRIMER_LEFT_2_PENALTY=2.664693
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_INTERNAL_2_PENALTY=2.236832
PRIMER_LEFT_2_SEQUENCE=CCTGGCAGAAGGGTGAAG
PRIMER_RIGHT_2_SEQUENCE=AAAAGGTGGGTCTTGCATTG
PRIMER_INTERNAL_2_SEQUENCE=CAAAGGCCCCAGACACAG
PRIMER_LEFT_2=14,18
PRIMER_RIGHT_2=96,20
PRIMER_INTERNAL_2=33,18
PRIMER_LEFT_2_TM=59.335
PRIMER_RIGHT_2_TM=59.971
PRIMER_INTERNAL_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=61.111
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=3.074875
PRIMER_LEFT_3_PENALTY=2.244684
PRIMER_RIGHT_3_PENALTY=0.830191
PRIMER_INTERNAL_3_PENALTY=1.730588
PRIMER_LEFT_3_SEQUENCE=TCACCTTTTCAGGCCCTG
PRIMER_RIGHT_3_SEQUENCE=TGTAAAAGGTGGGTCTTGCA
PRIMER_INTERNAL_3_SEQUENCE=AGAAGGGTGAAGGCAAAGG
PRIMER_LEFT_3=0,18
PRIMER_RIGHT_3=99,20
PRIMER_INTERNAL_3=20,19
PRIMER_LEFT_3_TM=59.755
PRIMER_RIGHT_3_TM=59.170
PRIMER_INTERNAL_3_TM=59.269
PRIMER_LEFT_3_GC_PERCENT=55.556
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=52.632
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=7.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=3.262372
PRIMER_LEFT_4_PENALTY=2.244684
PRIMER_RIGHT_4_PENALTY=1.017688
PRIMER_INTERNAL_4_PENALTY=1.730588
PRIMER_LEFT_4_SEQUENCE=TCACCTTTTCAGGCCCTG
PRIMER_RIGHT_4_SEQUENCE=TAAAAGGTGGGTCTTGCATTG
PRIMER_INTERNAL_4_SEQUENCE=AGAAGGGTGAAGGCAAAGG
PRIMER_LEFT_4=0,18
PRIMER_RIGHT_4=97,21
PRIMER_INTERNAL_4=20,19
PRIMER_LEFT_4_TM=59.755
PRIMER_RIGHT_4_TM=59.982
PRIMER_INTERNAL_4_TM=59.269
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=52.632
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=7.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH1123
SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTCAAAACTATTAAGGGNATAAATAAAAAGTNGGGCAAGTNCATTTTTGGTTTAAAATGNGACTNTTAAAGCAGAAGGGAGTNTCCTTCCCCCTCCCCCACAAAGAATATTTTATAGATGTGAAAATACTAAGAGATTAAAGTTTCAGCAGAAATAAATATCTCAAAGGAGGCACCATACTATTTCCTTTCCATATGTTTAGTANNTCATTTTCACACATCGTTTGTAGTCTCCTAAGTTTAGACAAATCATCTTAG
SEQUENCE_INCLUDED_REGION=34,256
P3_COMMENT=CL is CCTCCCCCACAAAGAATATT
P3_COMMENT=CR is AGGAGACTACAAACGATGTGTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.044631
PRIMER_LEFT_0_PENALTY=0.010529
PRIMER_RIGHT_0_PENALTY=2.034102
PRIMER_INTERNAL_0_PENALTY=8.987340
PRIMER_LEFT_0_SEQUENCE=CCCTCCCCCACAAAGAATAT
PRIMER_RIGHT_0_SEQUENCE=TGGTGCCTCCTTTGAGATATTT
PRIMER_INTERNAL_0_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
PRIMER_LEFT_0=123,20
PRIMER_RIGHT_0=209,22
PRIMER_INTERNAL_0=161,26
PRIMER_LEFT_0_TM=60.011
PRIMER_RIGHT_0_TM=59.966
PRIMER_INTERNAL_0_TM=57.013
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_INTERNAL_0_GC_PERCENT=30.769
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=5.8000
PRIMER_RIGHT_0_END_STABILITY=6.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=2.207274
PRIMER_LEFT_1_PENALTY=0.010529
PRIMER_RIGHT_1_PENALTY=2.196745
PRIMER_INTERNAL_1_PENALTY=8.987340
PRIMER_LEFT_1_SEQUENCE=CCCTCCCCCACAAAGAATAT
PRIMER_RIGHT_1_SEQUENCE=AGTATGGTGCCTCCTTTGAGA
PRIMER_INTERNAL_1_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
PRIMER_LEFT_1=123,20
PRIMER_RIGHT_1=213,21
PRIMER_INTERNAL_1=161,26
PRIMER_LEFT_1_TM=60.011
PRIMER_RIGHT_1_TM=58.803
PRIMER_INTERNAL_1_TM=57.013
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=30.769
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=2.273455
PRIMER_LEFT_2_PENALTY=0.010529
PRIMER_RIGHT_2_PENALTY=2.262926
PRIMER_INTERNAL_2_PENALTY=8.987340
PRIMER_LEFT_2_SEQUENCE=CCCTCCCCCACAAAGAATAT
PRIMER_RIGHT_2_SEQUENCE=GTATGGTGCCTCCTTTGAGA
PRIMER_INTERNAL_2_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
PRIMER_LEFT_2=123,20
PRIMER_RIGHT_2=212,20
PRIMER_INTERNAL_2=161,26
PRIMER_LEFT_2_TM=60.011
PRIMER_RIGHT_2_TM=57.737
PRIMER_INTERNAL_2_TM=57.013
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=30.769
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=8.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=2.351561
PRIMER_LEFT_3_PENALTY=0.010529
PRIMER_RIGHT_3_PENALTY=2.341032
PRIMER_INTERNAL_3_PENALTY=8.987340
PRIMER_LEFT_3_SEQUENCE=CCCTCCCCCACAAAGAATAT
PRIMER_RIGHT_3_SEQUENCE=TGGTGCCTCCTTTGAGATATT
PRIMER_INTERNAL_3_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
PRIMER_LEFT_3=123,20
PRIMER_RIGHT_3=209,21
PRIMER_INTERNAL_3=161,26
PRIMER_LEFT_3_TM=60.011
PRIMER_RIGHT_3_TM=58.659
PRIMER_INTERNAL_3_TM=57.013
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=87
PRIMER_PAIR_4_PENALTY=2.806077
PRIMER_LEFT_4_PENALTY=0.010529
PRIMER_RIGHT_4_PENALTY=2.795548
PRIMER_INTERNAL_4_PENALTY=8.987340
PRIMER_LEFT_4_SEQUENCE=CCCTCCCCCACAAAGAATAT
PRIMER_RIGHT_4_SEQUENCE=TGGTGCCTCCTTTGAGATAT
PRIMER_INTERNAL_4_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA
PRIMER_LEFT_4=123,20
PRIMER_RIGHT_4=209,20
PRIMER_INTERNAL_4=161,26
PRIMER_LEFT_4_TM=60.011
PRIMER_RIGHT_4_TM=57.204
PRIMER_INTERNAL_4_TM=57.013
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=30.769
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=5.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH1139
SEQUENCE_TEMPLATE=TTGCNNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTTGTNAAAGCCTCAGTGTNNCTTCAGAGCACAGGGCTTATTTTTACTTTTATGTCCTGACTTNNNNTGGGCCGCCTGAGTCATTCAGAAAAGGTGGCCCAGGGACATGAACACCATTTCTTTTCCTTAATTCCCATAATTAATTGCTTTTAAATTAGGGGCAATGACTCTTTGGCAAATNACCCTGCCAAATACTTGCTTACTCCCCTTTGTGAG
SEQUENCE_INCLUDED_REGION=34,220
P3_COMMENT=CL is TTCAGAGCACAGGGCTTATT
P3_COMMENT=CR is TAAGCAAGTATTTGGCAGGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.534736
PRIMER_LEFT_0_PENALTY=0.468413
PRIMER_RIGHT_0_PENALTY=0.066323
PRIMER_INTERNAL_0_PENALTY=1.225160
PRIMER_LEFT_0_SEQUENCE=GTCATTCAGAAAAGGTGGCC
PRIMER_RIGHT_0_SEQUENCE=CCAAAGAGTCATTGCCCCTA
PRIMER_INTERNAL_0_SEQUENCE=CAGGGACATGAACACCATTTC
PRIMER_LEFT_0=117,20
PRIMER_RIGHT_0=212,20
PRIMER_INTERNAL_0=137,21
PRIMER_LEFT_0_TM=59.532
PRIMER_RIGHT_0_TM=60.066
PRIMER_INTERNAL_0_TM=60.225
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=11.2000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=0.700807
PRIMER_LEFT_1_PENALTY=0.634484
PRIMER_RIGHT_1_PENALTY=0.066323
PRIMER_INTERNAL_1_PENALTY=6.124236
PRIMER_LEFT_1_SEQUENCE=CCAGGGACATGAACACCATT
PRIMER_RIGHT_1_SEQUENCE=CCAAAGAGTCATTGCCCCTA
PRIMER_INTERNAL_1_SEQUENCE=TTTCCTTAATTCCCATAATTAATTGC
PRIMER_LEFT_1=136,20
PRIMER_RIGHT_1=212,20
PRIMER_INTERNAL_1=159,26
PRIMER_LEFT_1_TM=60.634
PRIMER_RIGHT_1_TM=60.066
PRIMER_INTERNAL_1_TM=59.876
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=26.923
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=1.291483
PRIMER_LEFT_2_PENALTY=1.225160
PRIMER_RIGHT_2_PENALTY=0.066323
PRIMER_INTERNAL_2_PENALTY=6.124236
PRIMER_LEFT_2_SEQUENCE=CAGGGACATGAACACCATTTC
PRIMER_RIGHT_2_SEQUENCE=CCAAAGAGTCATTGCCCCTA
PRIMER_INTERNAL_2_SEQUENCE=TTTCCTTAATTCCCATAATTAATTGC
PRIMER_LEFT_2=137,21
PRIMER_RIGHT_2=212,20
PRIMER_INTERNAL_2=159,26
PRIMER_LEFT_2_TM=60.225
PRIMER_RIGHT_2_TM=60.066
PRIMER_INTERNAL_2_TM=59.876
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=26.923
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=1.436820
PRIMER_LEFT_3_PENALTY=1.370497
PRIMER_RIGHT_3_PENALTY=0.066323
PRIMER_INTERNAL_3_PENALTY=3.261219
PRIMER_LEFT_3_SEQUENCE=ATTCAGAAAAGGTGGCCCAG
PRIMER_RIGHT_3_SEQUENCE=CCAAAGAGTCATTGCCCCTA
PRIMER_INTERNAL_3_SEQUENCE=GACATGAACACCATTTCTTTTCC
PRIMER_LEFT_3=120,20
PRIMER_RIGHT_3=212,20
PRIMER_INTERNAL_3=141,23
PRIMER_LEFT_3_TM=61.370
PRIMER_RIGHT_3_TM=60.066
PRIMER_INTERNAL_3_TM=59.739
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=93
PRIMER_PAIR_4_PENALTY=1.511732
PRIMER_LEFT_4_PENALTY=1.445408
PRIMER_RIGHT_4_PENALTY=0.066323
PRIMER_INTERNAL_4_PENALTY=2.095574
PRIMER_LEFT_4_SEQUENCE=ATTCAGAAAAGGTGGCCCA
PRIMER_RIGHT_4_SEQUENCE=CCAAAGAGTCATTGCCCCTA
PRIMER_INTERNAL_4_SEQUENCE=GGGACATGAACACCATTTCTTT
PRIMER_LEFT_4=120,19
PRIMER_RIGHT_4=212,20
PRIMER_INTERNAL_4=139,22
PRIMER_LEFT_4_TM=60.445
PRIMER_RIGHT_4_TM=60.066
PRIMER_INTERNAL_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=47.368
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH114
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGATTTTTGTTTCAGAGGTTACCAGGAAAATAATTACACCAGTCTGACAATTTTAAATCAAGATTGAAGTATTGCTTTGGTTTCTTCTGAGATTTTGAGTNCCTAAATGAACATATTTGCAAAATTATTAAATATGAAAGTGGGTGGTAGTTTGGTTATTTTTAAAATCCTGAATAAATTTTTATATTTATATGGATTTTGAGGTGTCCCCATTTTATTTTTTCCTCTTTAACTCTGTCATGTTCATTTGCACCCCTTGTTCCCCTGACCTGTA
SEQUENCE_INCLUDED_REGION=35,275
P3_COMMENT=CL is CTGATTTTTGTTTCAGAGGTTACC
P3_COMMENT=CR is TACAGGTCAGGGGAACAAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.776366
PRIMER_LEFT_0_PENALTY=0.735594
PRIMER_RIGHT_0_PENALTY=0.040772
PRIMER_INTERNAL_0_PENALTY=1.438109
PRIMER_LEFT_0_SEQUENCE=TTTTGAGGTGTCCCCATTTT
PRIMER_RIGHT_0_SEQUENCE=TACAGGTCAGGGGAACAAGG
PRIMER_INTERNAL_0_SEQUENCE=TCTGTCATGTTCATTTGCACC
PRIMER_LEFT_0=232,20
PRIMER_RIGHT_0=309,20
PRIMER_INTERNAL_0=269,21
PRIMER_LEFT_0_TM=59.264
PRIMER_RIGHT_0_TM=59.959
PRIMER_INTERNAL_0_TM=59.562
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=0.990633
PRIMER_LEFT_1_PENALTY=0.949861
PRIMER_RIGHT_1_PENALTY=0.040772
PRIMER_INTERNAL_1_PENALTY=1.438109
PRIMER_LEFT_1_SEQUENCE=GGATTTTGAGGTGTCCCCAT
PRIMER_RIGHT_1_SEQUENCE=TACAGGTCAGGGGAACAAGG
PRIMER_INTERNAL_1_SEQUENCE=TCTGTCATGTTCATTTGCACC
PRIMER_LEFT_1=229,20
PRIMER_RIGHT_1=309,20
PRIMER_INTERNAL_1=269,21
PRIMER_LEFT_1_TM=60.950
PRIMER_RIGHT_1_TM=59.959
PRIMER_INTERNAL_1_TM=59.562
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=81
PRIMER_PAIR_2_PENALTY=0.990633
PRIMER_LEFT_2_PENALTY=0.949861
PRIMER_RIGHT_2_PENALTY=0.040772
PRIMER_INTERNAL_2_PENALTY=1.438109
PRIMER_LEFT_2_SEQUENCE=ATGGATTTTGAGGTGTCCCC
PRIMER_RIGHT_2_SEQUENCE=TACAGGTCAGGGGAACAAGG
PRIMER_INTERNAL_2_SEQUENCE=TCTGTCATGTTCATTTGCACC
PRIMER_LEFT_2=227,20
PRIMER_RIGHT_2=309,20
PRIMER_INTERNAL_2=269,21
PRIMER_LEFT_2_TM=60.950
PRIMER_RIGHT_2_TM=59.959
PRIMER_INTERNAL_2_TM=59.562
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=1.086832
PRIMER_LEFT_3_PENALTY=1.046061
PRIMER_RIGHT_3_PENALTY=0.040772
PRIMER_INTERNAL_3_PENALTY=1.438109
PRIMER_LEFT_3_SEQUENCE=GATTTTGAGGTGTCCCCATTT
PRIMER_RIGHT_3_SEQUENCE=TACAGGTCAGGGGAACAAGG
PRIMER_INTERNAL_3_SEQUENCE=TCTGTCATGTTCATTTGCACC
PRIMER_LEFT_3=230,21
PRIMER_RIGHT_3=309,20
PRIMER_INTERNAL_3=269,21
PRIMER_LEFT_3_TM=60.046
PRIMER_RIGHT_3_TM=59.959
PRIMER_INTERNAL_3_TM=59.562
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=1.349552
PRIMER_LEFT_4_PENALTY=1.308781
PRIMER_RIGHT_4_PENALTY=0.040772
PRIMER_INTERNAL_4_PENALTY=1.438109
PRIMER_LEFT_4_SEQUENCE=GATTTTGAGGTGTCCCCATT
PRIMER_RIGHT_4_SEQUENCE=TACAGGTCAGGGGAACAAGG
PRIMER_INTERNAL_4_SEQUENCE=TCTGTCATGTTCATTTGCACC
PRIMER_LEFT_4=230,20
PRIMER_RIGHT_4=309,20
PRIMER_INTERNAL_4=269,21
PRIMER_LEFT_4_TM=58.691
PRIMER_RIGHT_4_TM=59.959
PRIMER_INTERNAL_4_TM=59.562
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH1146
SEQUENCE_TEMPLATE=CAGGTCGACTCTAGAGGATCCCCTTTGAGTNNACATGCTTATAAGAGATTACATTTATAATACTTATGTGATATTAGTAAATTAAGCCTATTATATAACCAAGCATTAATTAGATTAGAATGCTTTTCTTTAGAATTAAGGATCAGATTATACAGTTGGGCTACAAACTTAGATGTCTGTAGTTGCTGGGCAAGTCAAATCAGCATGTGAAAGGGAAAGATAAGACAGTNTGGGCTGTGGTGGTGTGATAAAACTGGAGAACATGGACCCCTTAGGCCAGCCGCCACACACCAGATTTTACTAAGACACACTGGTGGGGA
SEQUENCE_INCLUDED_REGION=23,297
P3_COMMENT=CL is AGAATGCTTTTCTTTAGAATTAAGG
P3_COMMENT=CR is CCCCACCAGTGTGTCTTAGT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.568552
PRIMER_LEFT_0_PENALTY=0.259704
PRIMER_RIGHT_0_PENALTY=0.308849
PRIMER_INTERNAL_0_PENALTY=0.580157
PRIMER_LEFT_0_SEQUENCE=AGTTGCTGGGCAAGTCAAAT
PRIMER_RIGHT_0_SEQUENCE=TAAGGGGTCCATGTTCTCCA
PRIMER_INTERNAL_0_SEQUENCE=GGCTGTGGTGGTGTGATAAA
PRIMER_LEFT_0=180,20
PRIMER_RIGHT_0=273,20
PRIMER_INTERNAL_0=232,20
PRIMER_LEFT_0_TM=59.740
PRIMER_RIGHT_0_TM=60.309
PRIMER_INTERNAL_0_TM=59.420
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=0.587237
PRIMER_LEFT_1_PENALTY=0.259704
PRIMER_RIGHT_1_PENALTY=0.327533
PRIMER_INTERNAL_1_PENALTY=0.580157
PRIMER_LEFT_1_SEQUENCE=AGTTGCTGGGCAAGTCAAAT
PRIMER_RIGHT_1_SEQUENCE=GCCTAAGGGGTCCATGTTCT
PRIMER_INTERNAL_1_SEQUENCE=GGCTGTGGTGGTGTGATAAA
PRIMER_LEFT_1=180,20
PRIMER_RIGHT_1=276,20
PRIMER_INTERNAL_1=232,20
PRIMER_LEFT_1_TM=59.740
PRIMER_RIGHT_1_TM=60.328
PRIMER_INTERNAL_1_TM=59.420
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=0.758290
PRIMER_LEFT_2_PENALTY=0.449441
PRIMER_RIGHT_2_PENALTY=0.308849
PRIMER_INTERNAL_2_PENALTY=0.580157
PRIMER_LEFT_2_SEQUENCE=TGTAGTTGCTGGGCAAGTCA
PRIMER_RIGHT_2_SEQUENCE=TAAGGGGTCCATGTTCTCCA
PRIMER_INTERNAL_2_SEQUENCE=GGCTGTGGTGGTGTGATAAA
PRIMER_LEFT_2=177,20
PRIMER_RIGHT_2=273,20
PRIMER_INTERNAL_2=232,20
PRIMER_LEFT_2_TM=60.449
PRIMER_RIGHT_2_TM=60.309
PRIMER_INTERNAL_2_TM=59.420
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=0.776975
PRIMER_LEFT_3_PENALTY=0.449441
PRIMER_RIGHT_3_PENALTY=0.327533
PRIMER_INTERNAL_3_PENALTY=0.580157
PRIMER_LEFT_3_SEQUENCE=TGTAGTTGCTGGGCAAGTCA
PRIMER_RIGHT_3_SEQUENCE=GCCTAAGGGGTCCATGTTCT
PRIMER_INTERNAL_3_SEQUENCE=GGCTGTGGTGGTGTGATAAA
PRIMER_LEFT_3=177,20
PRIMER_RIGHT_3=276,20
PRIMER_INTERNAL_3=232,20
PRIMER_LEFT_3_TM=60.449
PRIMER_RIGHT_3_TM=60.328
PRIMER_INTERNAL_3_TM=59.420
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=0.845267
PRIMER_LEFT_4_PENALTY=0.536418
PRIMER_RIGHT_4_PENALTY=0.308849
PRIMER_INTERNAL_4_PENALTY=0.580157
PRIMER_LEFT_4_SEQUENCE=TAGTTGCTGGGCAAGTCAAA
PRIMER_RIGHT_4_SEQUENCE=TAAGGGGTCCATGTTCTCCA
PRIMER_INTERNAL_4_SEQUENCE=GGCTGTGGTGGTGTGATAAA
PRIMER_LEFT_4=179,20
PRIMER_RIGHT_4=273,20
PRIMER_INTERNAL_4=232,20
PRIMER_LEFT_4_TM=59.464
PRIMER_RIGHT_4_TM=60.309
PRIMER_INTERNAL_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH1147
SEQUENCE_TEMPLATE=AGGTCGACTCTAGAGGATCCCCCTACTAGGGAGGCTGAGGTGGGAGAATCACTTAAGCCCAGGAGGCAGAGGTTGCAGTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
SEQUENCE_INCLUDED_REGION=22,60
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1151
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATTTGCCAGTNCTGGGTGGGGGCAAATCCTCGAGGGGTNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
SEQUENCE_INCLUDED_REGION=36,37
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1156
SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTTACTTAATGGTAACCGTAGTAATAATGGCTATCTTTTAATGGTNNTGGGAGCGCTTAGTNTTTGACCACTTACTGAGTGCTAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
SEQUENCE_INCLUDED_REGION=36,88
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH1163
SEQUENCE_TEMPLATE=GCNTNCCTCCAGGTCGACTCTAGAGGATCCCCTGGTGTNNNCTGTCACTCTTCCCACTAGCCGGACTACACACAGACAGACTATACACTTCCAAGCCTCCTAATTTTGCCCATGTTGTTCTCTCTACCCAGAATACCTTTCCCTAAGCTGCCTGTAGGCCCTCTCAAAACATGCTAGTTCACTTTGTCAATGCCAGCAGAAGAATCTCTGTTGCTGCTATTAATGTCTTTCAAAGTCTCATGTGCTTTGGTCAGGCCTACCCAGGATAATCTCCATTTTNATTAACTCAAAGTCAACTGATTAGAGACCTTAATTATATCTGAA
SEQUENCE_INCLUDED_REGION=32,292
P3_COMMENT=CL is TGTCACTCTTCCCACTAGCC
P3_COMMENT=CR is GAGATTATCCTGGGTAGGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.283031
PRIMER_LEFT_0_PENALTY=0.069526
PRIMER_RIGHT_0_PENALTY=0.213506
PRIMER_INTERNAL_0_PENALTY=0.753123
PRIMER_LEFT_0_SEQUENCE=AGCCTCCTAATTTTGCCCAT
PRIMER_RIGHT_0_SEQUENCE=GCATGTTTTGAGAGGGCCTA
PRIMER_INTERNAL_0_SEQUENCE=ACCTTTCCCTAAGCTGCCTG
PRIMER_LEFT_0=93,20
PRIMER_RIGHT_0=173,20
PRIMER_INTERNAL_0=134,20
PRIMER_LEFT_0_TM=59.930
PRIMER_RIGHT_0_TM=60.214
PRIMER_INTERNAL_0_TM=60.753
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=0.283031
PRIMER_LEFT_1_PENALTY=0.069526
PRIMER_RIGHT_1_PENALTY=0.213506
PRIMER_INTERNAL_1_PENALTY=0.753123
PRIMER_LEFT_1_SEQUENCE=AGCCTCCTAATTTTGCCCAT
PRIMER_RIGHT_1_SEQUENCE=TAGCATGTTTTGAGAGGGCC
PRIMER_INTERNAL_1_SEQUENCE=CCTTTCCCTAAGCTGCCTGT
PRIMER_LEFT_1=93,20
PRIMER_RIGHT_1=175,20
PRIMER_INTERNAL_1=135,20
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=60.214
PRIMER_INTERNAL_1_TM=60.753
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.322471
PRIMER_LEFT_2_PENALTY=0.152999
PRIMER_RIGHT_2_PENALTY=0.169471
PRIMER_INTERNAL_2_PENALTY=4.996479
PRIMER_LEFT_2_SEQUENCE=TTCACTTTGTCAATGCCAGC
PRIMER_RIGHT_2_SEQUENCE=CCTGACCAAAGCACATGAGA
PRIMER_INTERNAL_2_SEQUENCE=TGTTGCTGCTATTAATGTCTTTCA
PRIMER_LEFT_2=177,20
PRIMER_RIGHT_2=254,20
PRIMER_INTERNAL_2=208,24
PRIMER_LEFT_2_TM=59.847
PRIMER_RIGHT_2_TM=59.831
PRIMER_INTERNAL_2_TM=59.004
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=0.382977
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=0.169471
PRIMER_INTERNAL_3_PENALTY=0.012060
PRIMER_LEFT_3_SEQUENCE=GGCCCTCTCAAAACATGCTA
PRIMER_RIGHT_3_SEQUENCE=CCTGACCAAAGCACATGAGA
PRIMER_INTERNAL_3_SEQUENCE=TTTGTCAATGCCAGCAGAAG
PRIMER_LEFT_3=156,20
PRIMER_RIGHT_3=254,20
PRIMER_INTERNAL_3=182,20
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=59.831
PRIMER_INTERNAL_3_TM=59.988
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=0.618913
PRIMER_LEFT_4_PENALTY=0.449441
PRIMER_RIGHT_4_PENALTY=0.169471
PRIMER_INTERNAL_4_PENALTY=4.996479
PRIMER_LEFT_4_SEQUENCE=CACTTTGTCAATGCCAGCAG
PRIMER_RIGHT_4_SEQUENCE=CCTGACCAAAGCACATGAGA
PRIMER_INTERNAL_4_SEQUENCE=TGTTGCTGCTATTAATGTCTTTCA
PRIMER_LEFT_4=179,20
PRIMER_RIGHT_4=254,20
PRIMER_INTERNAL_4=208,24
PRIMER_LEFT_4_TM=60.449
PRIMER_RIGHT_4_TM=59.831
PRIMER_INTERNAL_4_TM=59.004
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1166
SEQUENCE_TEMPLATE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCTTAAAAGTNNACTGCTGACTGATAGGGTNGAAAAGAAAAAAGAATGCCTGGGAGAAGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTNCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC
SEQUENCE_INCLUDED_REGION=33,60
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1177
SEQUENCE_TEMPLATE=GACTCTAGAGGATCCCCCTATATAATGGTNAAAATAATGTACAGTTAATGTGACTTGTTACAGGTTTTTACTCTAAGGTNATCACATAAATGAGTNNAGGATTCAAACAATGTTTGTTGTTACTGATGTTGCGAGACTGGCTTCAAGGTTATTTACAGAAGTGGTTCTAGGAAGAGTCATCTTTNGTCAGATGCTGACACCCACTGGTGAAAGATACAAACCTTTCCTAATAGAGGNTCTCTAGACATA
SEQUENCE_INCLUDED_REGION=17,232
P3_COMMENT=CL is AGGATTCAAACAATGTTTGTTG
P3_COMMENT=CR is GGAAAGGTTTGTATCTTTCACC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.085285
PRIMER_LEFT_0_PENALTY=0.955975
PRIMER_RIGHT_0_PENALTY=0.129310
PRIMER_INTERNAL_0_PENALTY=4.902432
PRIMER_LEFT_0_SEQUENCE=GATGTTGCGAGACTGGCTTC
PRIMER_RIGHT_0_SEQUENCE=TCTTTCACCAGTGGGTGTCA
PRIMER_INTERNAL_0_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
PRIMER_LEFT_0=124,20
PRIMER_RIGHT_0=213,20
PRIMER_INTERNAL_0=155,24
PRIMER_LEFT_0_TM=60.956
PRIMER_RIGHT_0_TM=60.129
PRIMER_INTERNAL_0_TM=59.098
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.833
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=1.245275
PRIMER_LEFT_1_PENALTY=1.115965
PRIMER_RIGHT_1_PENALTY=0.129310
PRIMER_INTERNAL_1_PENALTY=4.902432
PRIMER_LEFT_1_SEQUENCE=GTTGCGAGACTGGCTTCAAG
PRIMER_RIGHT_1_SEQUENCE=TCTTTCACCAGTGGGTGTCA
PRIMER_INTERNAL_1_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
PRIMER_LEFT_1=127,20
PRIMER_RIGHT_1=213,20
PRIMER_INTERNAL_1=155,24
PRIMER_LEFT_1_TM=61.116
PRIMER_RIGHT_1_TM=60.129
PRIMER_INTERNAL_1_TM=59.098
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=1.257461
PRIMER_LEFT_2_PENALTY=1.128150
PRIMER_RIGHT_2_PENALTY=0.129310
PRIMER_INTERNAL_2_PENALTY=4.902432
PRIMER_LEFT_2_SEQUENCE=GTTGCGAGACTGGCTTCAA
PRIMER_RIGHT_2_SEQUENCE=TCTTTCACCAGTGGGTGTCA
PRIMER_INTERNAL_2_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
PRIMER_LEFT_2=127,19
PRIMER_RIGHT_2=213,20
PRIMER_INTERNAL_2=155,24
PRIMER_LEFT_2_TM=60.128
PRIMER_RIGHT_2_TM=60.129
PRIMER_INTERNAL_2_TM=59.098
PRIMER_LEFT_2_GC_PERCENT=52.632
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.833
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=1.400001
PRIMER_LEFT_3_PENALTY=1.270690
PRIMER_RIGHT_3_PENALTY=0.129310
PRIMER_INTERNAL_3_PENALTY=4.902432
PRIMER_LEFT_3_SEQUENCE=TTGCGAGACTGGCTTCAAG
PRIMER_RIGHT_3_SEQUENCE=TCTTTCACCAGTGGGTGTCA
PRIMER_INTERNAL_3_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
PRIMER_LEFT_3=128,19
PRIMER_RIGHT_3=213,20
PRIMER_INTERNAL_3=155,24
PRIMER_LEFT_3_TM=60.271
PRIMER_RIGHT_3_TM=60.129
PRIMER_INTERNAL_3_TM=59.098
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.833
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=1.551559
PRIMER_LEFT_4_PENALTY=0.955975
PRIMER_RIGHT_4_PENALTY=0.595585
PRIMER_INTERNAL_4_PENALTY=4.902432
PRIMER_LEFT_4_SEQUENCE=GATGTTGCGAGACTGGCTTC
PRIMER_RIGHT_4_SEQUENCE=ACCAGTGGGTGTCAGCATCT
PRIMER_INTERNAL_4_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA
PRIMER_LEFT_4=124,20
PRIMER_RIGHT_4=207,20
PRIMER_INTERNAL_4=155,24
PRIMER_LEFT_4_TM=60.956
PRIMER_RIGHT_4_TM=60.596
PRIMER_INTERNAL_4_TM=59.098
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH1184
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTGTTCACATGAGGNNATGAGGACACTATCTTTTCTAGTNAGAAGTGAAGATGTGACAGGTTTTGTTATTGGAATGAGGGAGAACTTCGTAAGATATTCCCTTTTTTCGCCCCTACAAACCCAGCATCTTTAAAACCCCCTGTCTGAAAGCGTGACTCGTCTGTTGCCTTTCCACTCTGGTCAGTGGGTCCTCGAAAACACCATAACATGTTTCAATAATTCTGACAAACCCATGAAGATTTTTTAAAGTTAATGAATCATTCTTTCTCCTGAAAACACATAAAAACACGTTT
SEQUENCE_INCLUDED_REGION=35,292
P3_COMMENT=CL is GGAATGAGGGAGAACTTCGT
P3_COMMENT=CR is CGTGTTTTTATGTGTTTTCAGG
P3_COMMENT=CM is AGATATTCCCTTTTTTCGCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.398252
PRIMER_LEFT_0_PENALTY=0.248857
PRIMER_RIGHT_0_PENALTY=0.149395
PRIMER_INTERNAL_0_PENALTY=0.475706
PRIMER_LEFT_0_SEQUENCE=GATATTCCCTTTTTTCGCCC
PRIMER_RIGHT_0_SEQUENCE=GGAAAGGCAACAGACGAGTC
PRIMER_INTERNAL_0_SEQUENCE=AAAACCCCCTGTCTGAAAGC
PRIMER_LEFT_0=126,20
PRIMER_RIGHT_0=206,20
PRIMER_INTERNAL_0=165,20
PRIMER_LEFT_0_TM=59.751
PRIMER_RIGHT_0_TM=59.851
PRIMER_INTERNAL_0_TM=60.476
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=12.9000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=0.401799
PRIMER_LEFT_1_PENALTY=0.248857
PRIMER_RIGHT_1_PENALTY=0.152942
PRIMER_INTERNAL_1_PENALTY=0.230410
PRIMER_LEFT_1_SEQUENCE=GATATTCCCTTTTTTCGCCC
PRIMER_RIGHT_1_SEQUENCE=AGGACCCACTGACCAGAGTG
PRIMER_INTERNAL_1_SEQUENCE=CTGAAAGCGTGACTCGTCTG
PRIMER_LEFT_1=126,20
PRIMER_RIGHT_1=225,20
PRIMER_INTERNAL_1=177,20
PRIMER_LEFT_1_TM=59.751
PRIMER_RIGHT_1_TM=60.153
PRIMER_INTERNAL_1_TM=59.770
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=0.417734
PRIMER_LEFT_2_PENALTY=0.187324
PRIMER_RIGHT_2_PENALTY=0.230410
PRIMER_INTERNAL_2_PENALTY=0.248857
PRIMER_LEFT_2_SEQUENCE=TGGAATGAGGGAGAACTTCG
PRIMER_RIGHT_2_SEQUENCE=CGAGTCACGCTTTCAGACAG
PRIMER_INTERNAL_2_SEQUENCE=GATATTCCCTTTTTTCGCCC
PRIMER_LEFT_2=103,20
PRIMER_RIGHT_2=192,20
PRIMER_INTERNAL_2=126,20
PRIMER_LEFT_2_TM=60.187
PRIMER_RIGHT_2_TM=59.770
PRIMER_INTERNAL_2_TM=59.751
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=0.417734
PRIMER_LEFT_3_PENALTY=0.187324
PRIMER_RIGHT_3_PENALTY=0.230410
PRIMER_INTERNAL_3_PENALTY=0.248857
PRIMER_LEFT_3_SEQUENCE=TGGAATGAGGGAGAACTTCG
PRIMER_RIGHT_3_SEQUENCE=CAGACGAGTCACGCTTTCAG
PRIMER_INTERNAL_3_SEQUENCE=GATATTCCCTTTTTTCGCCC
PRIMER_LEFT_3=103,20
PRIMER_RIGHT_3=196,20
PRIMER_INTERNAL_3=126,20
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=59.770
PRIMER_INTERNAL_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=0.545579
PRIMER_LEFT_4_PENALTY=0.248857
PRIMER_RIGHT_4_PENALTY=0.296722
PRIMER_INTERNAL_4_PENALTY=0.230410
PRIMER_LEFT_4_SEQUENCE=GATATTCCCTTTTTTCGCCC
PRIMER_RIGHT_4_SEQUENCE=GACCAGAGTGGAAAGGCAAC
PRIMER_INTERNAL_4_SEQUENCE=CTGTCTGAAAGCGTGACTCG
PRIMER_LEFT_4=126,20
PRIMER_RIGHT_4=215,20
PRIMER_INTERNAL_4=173,20
PRIMER_LEFT_4_TM=59.751
PRIMER_RIGHT_4_TM=59.703
PRIMER_INTERNAL_4_TM=59.770
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=12.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH1185
SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTAAGTNNAGAATGTTTTTTTTTCAGGAAACCTCTACAATGCAGATGCTAGAGATGAGGGGTACCGAGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG
SEQUENCE_INCLUDED_REGION=33,57
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1186
SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACCTGGTACNNCNGNANTGANTGATCCGGATAAGGACAAAAAACGCCAGAAGAAAAGAGGCATTTTCCCCAAAGTAGCAACAAATATCATGAGAGCATGGCTCTTCCAGCATCTCACAGTAAGTCAAGATGAAAGAGNNAAAAAAAATAGTTTTTGTTGTTTTGCTAGCAAGTTATACGGTCTCATACTGATTTTTTTTTAANCTAAGGTGCCTATTTTCTTATTCTTGAAATCTGATGACTAACAGACTAGTTAAAGTTTTTTTTTTCATCAACTATTACCATCCAA
SEQUENCE_INCLUDED_REGION=35,292
P3_COMMENT=CL is TGATCCGGATAAGGACAAAA
P3_COMMENT=CR is TCAAGAATAAGAAAATAGGCACC
P3_COMMENT=CM is ACGCCAGAAGAAAAGAGGCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.141189
PRIMER_LEFT_0_PENALTY=0.227356
PRIMER_RIGHT_0_PENALTY=0.913833
PRIMER_INTERNAL_0_PENALTY=0.059875
PRIMER_LEFT_0_SEQUENCE=GGACAAAAAACGCCAGAAGA
PRIMER_RIGHT_0_SEQUENCE=TGGAAGAGCCATGCTCTCAT
PRIMER_INTERNAL_0_SEQUENCE=AGAGGCATTTTCCCCAAAGT
PRIMER_LEFT_0=72,20
PRIMER_RIGHT_0=146,20
PRIMER_INTERNAL_0=94,20
PRIMER_LEFT_0_TM=60.227
PRIMER_RIGHT_0_TM=60.914
PRIMER_INTERNAL_0_TM=59.940
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=1.141189
PRIMER_LEFT_1_PENALTY=0.227356
PRIMER_RIGHT_1_PENALTY=0.913833
PRIMER_INTERNAL_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=GGACAAAAAACGCCAGAAGA
PRIMER_RIGHT_1_SEQUENCE=GAGATGCTGGAAGAGCCATG
PRIMER_INTERNAL_1_SEQUENCE=AGAGGCATTTTCCCCAAAGT
PRIMER_LEFT_1=72,20
PRIMER_RIGHT_1=153,20
PRIMER_INTERNAL_1=94,20
PRIMER_LEFT_1_TM=60.227
PRIMER_RIGHT_1_TM=60.914
PRIMER_INTERNAL_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=1.141189
PRIMER_LEFT_2_PENALTY=0.227356
PRIMER_RIGHT_2_PENALTY=0.913833
PRIMER_INTERNAL_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=GGACAAAAAACGCCAGAAGA
PRIMER_RIGHT_2_SEQUENCE=TGAGATGCTGGAAGAGCCAT
PRIMER_INTERNAL_2_SEQUENCE=AGAGGCATTTTCCCCAAAGT
PRIMER_LEFT_2=72,20
PRIMER_RIGHT_2=154,20
PRIMER_INTERNAL_2=94,20
PRIMER_LEFT_2_TM=60.227
PRIMER_RIGHT_2_TM=60.914
PRIMER_INTERNAL_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=1.189582
PRIMER_LEFT_3_PENALTY=0.275749
PRIMER_RIGHT_3_PENALTY=0.913833
PRIMER_INTERNAL_3_PENALTY=0.059875
PRIMER_LEFT_3_SEQUENCE=AAGGACAAAAAACGCCAGAA
PRIMER_RIGHT_3_SEQUENCE=GGAAGAGCCATGCTCTCATG
PRIMER_INTERNAL_3_SEQUENCE=AGAGGCATTTTCCCCAAAGT
PRIMER_LEFT_3=70,20
PRIMER_RIGHT_3=145,20
PRIMER_INTERNAL_3=94,20
PRIMER_LEFT_3_TM=59.724
PRIMER_RIGHT_3_TM=60.914
PRIMER_INTERNAL_3_TM=59.940
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=1.189582
PRIMER_LEFT_4_PENALTY=0.275749
PRIMER_RIGHT_4_PENALTY=0.913833
PRIMER_INTERNAL_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=AAGGACAAAAAACGCCAGAA
PRIMER_RIGHT_4_SEQUENCE=TGGAAGAGCCATGCTCTCAT
PRIMER_INTERNAL_4_SEQUENCE=AGAGGCATTTTCCCCAAAGT
PRIMER_LEFT_4=70,20
PRIMER_RIGHT_4=146,20
PRIMER_INTERNAL_4=94,20
PRIMER_LEFT_4_TM=59.724
PRIMER_RIGHT_4_TM=60.914
PRIMER_INTERNAL_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH1187
SEQUENCE_TEMPLATE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTAGAAAAGAAATAAGATACTGGGTCATAGGATTGCCCCTGCTTCCTGAAAGCATCCAATCCAGAGTAAAGTTCCATTTCTTTGAGGCTCCTCCCAAATACTCTATCACAAGTNCAAATCCTATAATAAGGCTTTTCTAATGCACTCTCACCGAGAAGCCTTATGGTCCCCATGGTGTGTGTTCTCCCTTGTTGCAAAGACTCAATAAACTGAAACTTGTTCAGTTCCAGGTATTCCTGGTTGTCTTGATTGGAGGGAATTGATAAGTCTATAGGGGTA
SEQUENCE_INCLUDED_REGION=33,279
P3_COMMENT=CL is TACTGGGTCATAGGATTGCC
P3_COMMENT=CR is TCAATTCCCTCCAATCAAGA
P3_COMMENT=CM is CTGCTTCCTGAAAGCATCCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.370640
PRIMER_LEFT_0_PENALTY=0.273747
PRIMER_RIGHT_0_PENALTY=0.096893
PRIMER_INTERNAL_0_PENALTY=3.115731
PRIMER_LEFT_0_SEQUENCE=GTGTGTTCTCCCTTGTTGCA
PRIMER_RIGHT_0_SEQUENCE=TCCCTCCAATCAAGACAACC
PRIMER_INTERNAL_0_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG
PRIMER_LEFT_0=209,20
PRIMER_RIGHT_0=290,20
PRIMER_INTERNAL_0=242,22
PRIMER_LEFT_0_TM=59.726
PRIMER_RIGHT_0_TM=59.903
PRIMER_INTERNAL_0_TM=58.884
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=0.370640
PRIMER_LEFT_1_PENALTY=0.273747
PRIMER_RIGHT_1_PENALTY=0.096893
PRIMER_INTERNAL_1_PENALTY=3.115731
PRIMER_LEFT_1_SEQUENCE=TGTGTGTTCTCCCTTGTTGC
PRIMER_RIGHT_1_SEQUENCE=TCCCTCCAATCAAGACAACC
PRIMER_INTERNAL_1_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG
PRIMER_LEFT_1=208,20
PRIMER_RIGHT_1=290,20
PRIMER_INTERNAL_1=242,22
PRIMER_LEFT_1_TM=59.726
PRIMER_RIGHT_1_TM=59.903
PRIMER_INTERNAL_1_TM=58.884
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.374878
PRIMER_LEFT_2_PENALTY=0.277985
PRIMER_RIGHT_2_PENALTY=0.096893
PRIMER_INTERNAL_2_PENALTY=3.115731
PRIMER_LEFT_2_SEQUENCE=TGTGTTCTCCCTTGTTGCAA
PRIMER_RIGHT_2_SEQUENCE=TCCCTCCAATCAAGACAACC
PRIMER_INTERNAL_2_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG
PRIMER_LEFT_2=210,20
PRIMER_RIGHT_2=290,20
PRIMER_INTERNAL_2=242,22
PRIMER_LEFT_2_TM=60.278
PRIMER_RIGHT_2_TM=59.903
PRIMER_INTERNAL_2_TM=58.884
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=0.518779
PRIMER_LEFT_3_PENALTY=0.348730
PRIMER_RIGHT_3_PENALTY=0.170049
PRIMER_INTERNAL_3_PENALTY=7.082104
PRIMER_LEFT_3_SEQUENCE=ATTTCTTTGAGGCTCCTCCC
PRIMER_RIGHT_3_SEQUENCE=CCATAAGGCTTCTCGGTGAG
PRIMER_INTERNAL_3_SEQUENCE=TCCTATAATAAGGCTTTTCTAATGCA
PRIMER_LEFT_3=108,20
PRIMER_RIGHT_3=198,20
PRIMER_INTERNAL_3=151,26
PRIMER_LEFT_3_TM=59.651
PRIMER_RIGHT_3_TM=59.830
PRIMER_INTERNAL_3_TM=58.918
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=0.530467
PRIMER_LEFT_4_PENALTY=0.360418
PRIMER_RIGHT_4_PENALTY=0.170049
PRIMER_INTERNAL_4_PENALTY=7.082104
PRIMER_LEFT_4_SEQUENCE=TTTGAGGCTCCTCCCAAATA
PRIMER_RIGHT_4_SEQUENCE=CCATAAGGCTTCTCGGTGAG
PRIMER_INTERNAL_4_SEQUENCE=TCCTATAATAAGGCTTTTCTAATGCA
PRIMER_LEFT_4=113,20
PRIMER_RIGHT_4=198,20
PRIMER_INTERNAL_4=151,26
PRIMER_LEFT_4_TM=59.640
PRIMER_RIGHT_4_TM=59.830
PRIMER_INTERNAL_4_TM=58.918
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=30.769
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH1188
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAGTNTTAAATGTTCTCATGAAATTATTCATTTTCTAAAACAAAGTTTTCCTTCCATGAACCAAACAGGAAAAATCCTTTTCATTCTTCGGTCTCTATGGCCCAAAATTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTG
SEQUENCE_INCLUDED_REGION=36,114
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.773284
PRIMER_LEFT_0_PENALTY=3.933413
PRIMER_RIGHT_0_PENALTY=1.839872
PRIMER_INTERNAL_0_PENALTY=2.986824
PRIMER_LEFT_0_SEQUENCE=AAAACAAAGTTTTCCTTCCATGA
PRIMER_RIGHT_0_SEQUENCE=TGAATTTTGGGCCATAGAGA
PRIMER_INTERNAL_0_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
PRIMER_LEFT_0=75,23
PRIMER_RIGHT_0=149,20
PRIMER_INTERNAL_0=99,22
PRIMER_LEFT_0_TM=59.067
PRIMER_RIGHT_0_TM=58.160
PRIMER_INTERNAL_0_TM=59.013
PRIMER_LEFT_0_GC_PERCENT=30.435
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=5.900543
PRIMER_LEFT_1_PENALTY=3.933413
PRIMER_RIGHT_1_PENALTY=1.967130
PRIMER_INTERNAL_1_PENALTY=2.986824
PRIMER_LEFT_1_SEQUENCE=AAAACAAAGTTTTCCTTCCATGA
PRIMER_RIGHT_1_SEQUENCE=TGAATTTTGGGCCATAGAGAC
PRIMER_INTERNAL_1_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
PRIMER_LEFT_1=75,23
PRIMER_RIGHT_1=149,21
PRIMER_INTERNAL_1=99,22
PRIMER_LEFT_1_TM=59.067
PRIMER_RIGHT_1_TM=59.033
PRIMER_INTERNAL_1_TM=59.013
PRIMER_LEFT_1_GC_PERCENT=30.435
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_INTERNAL_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=6.077816
PRIMER_LEFT_2_PENALTY=4.237945
PRIMER_RIGHT_2_PENALTY=1.839872
PRIMER_INTERNAL_2_PENALTY=2.986824
PRIMER_LEFT_2_SEQUENCE=AAAACAAAGTTTTCCTTCCATGAA
PRIMER_RIGHT_2_SEQUENCE=TGAATTTTGGGCCATAGAGA
PRIMER_INTERNAL_2_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
PRIMER_LEFT_2=75,24
PRIMER_RIGHT_2=149,20
PRIMER_INTERNAL_2=99,22
PRIMER_LEFT_2_TM=60.238
PRIMER_RIGHT_2_TM=58.160
PRIMER_INTERNAL_2_TM=59.013
PRIMER_LEFT_2_GC_PERCENT=29.167
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=36.364
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=6.102398
PRIMER_LEFT_3_PENALTY=4.893307
PRIMER_RIGHT_3_PENALTY=1.209091
PRIMER_INTERNAL_3_PENALTY=2.986824
PRIMER_LEFT_3_SEQUENCE=TAAAACAAAGTTTTCCTTCCATGA
PRIMER_RIGHT_3_SEQUENCE=GAATTTTGGGCCATAGAGACC
PRIMER_INTERNAL_3_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
PRIMER_LEFT_3=74,24
PRIMER_RIGHT_3=148,21
PRIMER_INTERNAL_3=99,22
PRIMER_LEFT_3_TM=59.107
PRIMER_RIGHT_3_TM=59.791
PRIMER_INTERNAL_3_TM=59.013
PRIMER_LEFT_3_GC_PERCENT=29.167
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=36.364
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=6.205074
PRIMER_LEFT_4_PENALTY=4.237945
PRIMER_RIGHT_4_PENALTY=1.967130
PRIMER_INTERNAL_4_PENALTY=2.986824
PRIMER_LEFT_4_SEQUENCE=AAAACAAAGTTTTCCTTCCATGAA
PRIMER_RIGHT_4_SEQUENCE=TGAATTTTGGGCCATAGAGAC
PRIMER_INTERNAL_4_SEQUENCE=CCAAACAGGAAAAATCCTTTTC
PRIMER_LEFT_4=75,24
PRIMER_RIGHT_4=149,21
PRIMER_INTERNAL_4=99,22
PRIMER_LEFT_4_TM=60.238
PRIMER_RIGHT_4_TM=59.033
PRIMER_INTERNAL_4_TM=59.013
PRIMER_LEFT_4_GC_PERCENT=29.167
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=36.364
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH119
SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGACAGATTGAGTNTAGAGAAGTGAAAATACCTTCTTCCTCCAGGCCAGGTTAGAGAAAATCAGGCAAAGGAAACAAATTTCAGTGCTGTGTGAAATGGTCTGACATGGACATGGATACCTGTATAGATAAANCCAAGATCTGAACAAAAGTTACCCATTTTGGAGACAGAATGTCCTCCCCCAGAAAGGTTCATGNTAATCCTACCAG
SEQUENCE_INCLUDED_REGION=35,212
P3_COMMENT=CL is GGCAAAGGAAACAAATTTCA
P3_COMMENT=CR is CATTCTGTCTCCAAAATGGG
P3_COMMENT=CM is GTGTGAAATGGTCTGACATGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.864013
PRIMER_LEFT_0_PENALTY=0.815754
PRIMER_RIGHT_0_PENALTY=0.048259
PRIMER_INTERNAL_0_PENALTY=4.261278
PRIMER_LEFT_0_SEQUENCE=ATGGTCTGACATGGACATGG
PRIMER_RIGHT_0_SEQUENCE=GGGAGGACATTCTGTCTCCA
PRIMER_INTERNAL_0_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
PRIMER_LEFT_0=133,20
PRIMER_RIGHT_0=218,20
PRIMER_INTERNAL_0=171,24
PRIMER_LEFT_0_TM=59.184
PRIMER_RIGHT_0_TM=60.048
PRIMER_INTERNAL_0_TM=60.261
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=1.008672
PRIMER_LEFT_1_PENALTY=0.192918
PRIMER_RIGHT_1_PENALTY=0.815754
PRIMER_INTERNAL_1_PENALTY=2.271131
PRIMER_LEFT_1_SEQUENCE=TCCAGGCCAGGTTAGAGAAA
PRIMER_RIGHT_1_SEQUENCE=CCATGTCCATGTCAGACCAT
PRIMER_INTERNAL_1_SEQUENCE=GGCAAAGGAAACAAATTTCAG
PRIMER_LEFT_1=77,20
PRIMER_RIGHT_1=152,20
PRIMER_INTERNAL_1=101,21
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=59.184
PRIMER_INTERNAL_1_TM=58.729
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=1.030658
PRIMER_LEFT_2_PENALTY=0.982399
PRIMER_RIGHT_2_PENALTY=0.048259
PRIMER_INTERNAL_2_PENALTY=4.261278
PRIMER_LEFT_2_SEQUENCE=TGGTCTGACATGGACATGGA
PRIMER_RIGHT_2_SEQUENCE=GGGAGGACATTCTGTCTCCA
PRIMER_INTERNAL_2_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
PRIMER_LEFT_2=134,20
PRIMER_RIGHT_2=218,20
PRIMER_INTERNAL_2=171,24
PRIMER_LEFT_2_TM=60.982
PRIMER_RIGHT_2_TM=60.048
PRIMER_INTERNAL_2_TM=60.261
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=1.097237
PRIMER_LEFT_3_PENALTY=1.048978
PRIMER_RIGHT_3_PENALTY=0.048259
PRIMER_INTERNAL_3_PENALTY=4.261278
PRIMER_LEFT_3_SEQUENCE=TGAAATGGTCTGACATGGACA
PRIMER_RIGHT_3_SEQUENCE=GGGAGGACATTCTGTCTCCA
PRIMER_INTERNAL_3_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
PRIMER_LEFT_3=129,21
PRIMER_RIGHT_3=218,20
PRIMER_INTERNAL_3=171,24
PRIMER_LEFT_3_TM=59.951
PRIMER_RIGHT_3_TM=60.048
PRIMER_INTERNAL_3_TM=60.261
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=1.097237
PRIMER_LEFT_4_PENALTY=1.048978
PRIMER_RIGHT_4_PENALTY=0.048259
PRIMER_INTERNAL_4_PENALTY=4.261278
PRIMER_LEFT_4_SEQUENCE=TGTGAAATGGTCTGACATGGA
PRIMER_RIGHT_4_SEQUENCE=GGGAGGACATTCTGTCTCCA
PRIMER_INTERNAL_4_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC
PRIMER_LEFT_4=127,21
PRIMER_RIGHT_4=218,20
PRIMER_INTERNAL_4=171,24
PRIMER_LEFT_4_TM=59.951
PRIMER_RIGHT_4_TM=60.048
PRIMER_INTERNAL_4_TM=60.261
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH1190
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTATACATCTGTNTATCTTTCTATTTCTCTATCTATCTATAAACTCCAATATACAAATTGGGAGAAAAAGAGTTGTTCTTGTGTTGGTGTGGATAAAGAAGAAAATAGATAGAGAGAAGCCAGTGATAAAAGCAACATCAAAGTAAATTACATTAAATTTCAATAAAATATAANGACCCAAACACACCAGAAACTGCCAATCATATATGACGTCTGCTCTTATTCTCTGCTTTTNGTCTTGTACATTTTCTAAGCAATGGACAATTTCTTCGTCAACAAAATTCCATCTTCTCTCAAATATGCAAG
SEQUENCE_INCLUDED_REGION=36,306
P3_COMMENT=CL is TTGTTCTTGTGTTGGTGTGG
P3_COMMENT=CR is CGAAGAAATTGTCCATTGCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.247663
PRIMER_LEFT_0_PENALTY=0.812957
PRIMER_RIGHT_0_PENALTY=1.434707
PRIMER_INTERNAL_0_PENALTY=3.629138
PRIMER_LEFT_0_SEQUENCE=AACACACCAGAAACTGCCAA
PRIMER_RIGHT_0_SEQUENCE=GACGAAGAAATTGTCCATTGC
PRIMER_INTERNAL_0_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
PRIMER_LEFT_0=216,20
PRIMER_RIGHT_0=309,21
PRIMER_INTERNAL_0=244,22
PRIMER_LEFT_0_TM=59.187
PRIMER_RIGHT_0_TM=59.565
PRIMER_INTERNAL_0_TM=58.371
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=2.247663
PRIMER_LEFT_1_PENALTY=0.812957
PRIMER_RIGHT_1_PENALTY=1.434707
PRIMER_INTERNAL_1_PENALTY=3.629138
PRIMER_LEFT_1_SEQUENCE=AAACACACCAGAAACTGCCA
PRIMER_RIGHT_1_SEQUENCE=GACGAAGAAATTGTCCATTGC
PRIMER_INTERNAL_1_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
PRIMER_LEFT_1=215,20
PRIMER_RIGHT_1=309,21
PRIMER_INTERNAL_1=244,22
PRIMER_LEFT_1_TM=59.187
PRIMER_RIGHT_1_TM=59.565
PRIMER_INTERNAL_1_TM=58.371
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=2.247663
PRIMER_LEFT_2_PENALTY=0.812957
PRIMER_RIGHT_2_PENALTY=1.434707
PRIMER_INTERNAL_2_PENALTY=3.629138
PRIMER_LEFT_2_SEQUENCE=CAAACACACCAGAAACTGCC
PRIMER_RIGHT_2_SEQUENCE=GACGAAGAAATTGTCCATTGC
PRIMER_INTERNAL_2_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
PRIMER_LEFT_2=214,20
PRIMER_RIGHT_2=309,21
PRIMER_INTERNAL_2=244,22
PRIMER_LEFT_2_TM=59.187
PRIMER_RIGHT_2_TM=59.565
PRIMER_INTERNAL_2_TM=58.371
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=2.247663
PRIMER_LEFT_3_PENALTY=0.812957
PRIMER_RIGHT_3_PENALTY=1.434707
PRIMER_INTERNAL_3_PENALTY=3.629138
PRIMER_LEFT_3_SEQUENCE=CCAAACACACCAGAAACTGC
PRIMER_RIGHT_3_SEQUENCE=GACGAAGAAATTGTCCATTGC
PRIMER_INTERNAL_3_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
PRIMER_LEFT_3=213,20
PRIMER_RIGHT_3=309,21
PRIMER_INTERNAL_3=244,22
PRIMER_LEFT_3_TM=59.187
PRIMER_RIGHT_3_TM=59.565
PRIMER_INTERNAL_3_TM=58.371
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=2.295870
PRIMER_LEFT_4_PENALTY=0.861164
PRIMER_RIGHT_4_PENALTY=1.434707
PRIMER_INTERNAL_4_PENALTY=3.629138
PRIMER_LEFT_4_SEQUENCE=ACACCAGAAACTGCCAATCA
PRIMER_RIGHT_4_SEQUENCE=GACGAAGAAATTGTCCATTGC
PRIMER_INTERNAL_4_SEQUENCE=GACGTCTGCTCTTATTCTCTGC
PRIMER_LEFT_4=219,20
PRIMER_RIGHT_4=309,21
PRIMER_INTERNAL_4=244,22
PRIMER_LEFT_4_TM=59.139
PRIMER_RIGHT_4_TM=59.565
PRIMER_INTERNAL_4_TM=58.371
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH1192
SEQUENCE_TEMPLATE=GCTTGCATNNCTNCAGGTCGACTCTAGAGGATCCCCTGCAGGACTCTTTGAAGAGTAAATTGAACAATGATGTCAGGCACCAAGAAAGACGCCTGGAAGAGACCAGGGCTCCATGAGAGTNAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGA
SEQUENCE_INCLUDED_REGION=36,87
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.745299
PRIMER_LEFT_0_PENALTY=4.674205
PRIMER_RIGHT_0_PENALTY=1.071094
PRIMER_INTERNAL_0_PENALTY=0.032945
PRIMER_LEFT_0_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
PRIMER_RIGHT_0_SEQUENCE=GAGCCCTGGTCTCTTCCAG
PRIMER_INTERNAL_0_SEQUENCE=ACAATGATGTCAGGCACCAA
PRIMER_LEFT_0=36,23
PRIMER_RIGHT_0=110,19
PRIMER_INTERNAL_0=63,20
PRIMER_LEFT_0_TM=58.326
PRIMER_RIGHT_0_TM=59.929
PRIMER_INTERNAL_0_TM=59.967
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=10.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=5.881064
PRIMER_LEFT_1_PENALTY=4.674205
PRIMER_RIGHT_1_PENALTY=1.206859
PRIMER_INTERNAL_1_PENALTY=0.032945
PRIMER_LEFT_1_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
PRIMER_RIGHT_1_SEQUENCE=CATGGAGCCCTGGTCTCTT
PRIMER_INTERNAL_1_SEQUENCE=ACAATGATGTCAGGCACCAA
PRIMER_LEFT_1=36,23
PRIMER_RIGHT_1=114,19
PRIMER_INTERNAL_1=63,20
PRIMER_LEFT_1_TM=58.326
PRIMER_RIGHT_1_TM=60.207
PRIMER_INTERNAL_1_TM=59.967
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=6.020542
PRIMER_LEFT_2_PENALTY=4.674205
PRIMER_RIGHT_2_PENALTY=1.346336
PRIMER_INTERNAL_2_PENALTY=0.032945
PRIMER_LEFT_2_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
PRIMER_RIGHT_2_SEQUENCE=ACTCTCATGGAGCCCTGGT
PRIMER_INTERNAL_2_SEQUENCE=ACAATGATGTCAGGCACCAA
PRIMER_LEFT_2=36,23
PRIMER_RIGHT_2=119,19
PRIMER_INTERNAL_2=63,20
PRIMER_LEFT_2_TM=58.326
PRIMER_RIGHT_2_TM=59.654
PRIMER_INTERNAL_2_TM=59.967
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=9.00
PRIMER_PAIR_2_COMPL_END=7.00
PRIMER_PAIR_2_PRODUCT_SIZE=84
PRIMER_PAIR_3_PENALTY=6.219599
PRIMER_LEFT_3_PENALTY=5.148505
PRIMER_RIGHT_3_PENALTY=1.071094
PRIMER_INTERNAL_3_PENALTY=0.032945
PRIMER_LEFT_3_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAATT
PRIMER_RIGHT_3_SEQUENCE=GAGCCCTGGTCTCTTCCAG
PRIMER_INTERNAL_3_SEQUENCE=ACAATGATGTCAGGCACCAA
PRIMER_LEFT_3=36,25
PRIMER_RIGHT_3=110,19
PRIMER_INTERNAL_3=63,20
PRIMER_LEFT_3_TM=59.851
PRIMER_RIGHT_3_TM=59.929
PRIMER_INTERNAL_3_TM=59.967
PRIMER_LEFT_3_GC_PERCENT=36.000
PRIMER_RIGHT_3_GC_PERCENT=63.158
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=6.295713
PRIMER_LEFT_4_PENALTY=4.674205
PRIMER_RIGHT_4_PENALTY=1.621508
PRIMER_INTERNAL_4_PENALTY=0.032945
PRIMER_LEFT_4_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA
PRIMER_RIGHT_4_SEQUENCE=ACTCTCATGGAGCCCTGGTC
PRIMER_INTERNAL_4_SEQUENCE=ACAATGATGTCAGGCACCAA
PRIMER_LEFT_4=36,23
PRIMER_RIGHT_4=119,20
PRIMER_INTERNAL_4=63,20
PRIMER_LEFT_4_TM=58.326
PRIMER_RIGHT_4_TM=61.622
PRIMER_INTERNAL_4_TM=59.967
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=10.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=9.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH1193
SEQUENCE_TEMPLATE=CCTTTTGTNTCCTCATTTGTNAAGTGGGAAANATGGAATCTATCTAAGGCTTGGNAGGTCACATAAAGAAAGGCACTAGAATGATTTCNTTCTNTCTCCTCACCACCCGCTCGACCCTCCCTCTNACTGCCTTNAGGGTTGGCGAAAGANAGGAGACAGGCGAGGTTANGAGATTTNATAGGGCAGGTTTCACAAGGTCAGNGAAATTACATTAANTTAACACTGNCTACATTAAACATGGAATGCCTTTTTGGNGTGTTGGTCCCCACACTT
SEQUENCE_INCLUDED_REGION=0,273
P3_COMMENT=CL is ATGGAATCTATCTAAGGCTTGG
P3_COMMENT=CR is CAAAAAGGCATTCCATGTTT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.347509
PRIMER_LEFT_0_PENALTY=4.939568
PRIMER_RIGHT_0_PENALTY=0.407941
PRIMER_INTERNAL_0_PENALTY=4.452854
PRIMER_LEFT_0_SEQUENCE=AGGTCACATAAAGAAAGGCACT
PRIMER_RIGHT_0_SEQUENCE=ACCTTGTGAAACCTGCCCTA
PRIMER_INTERNAL_0_SEQUENCE=TCTCCTCACCACCCGCTC
PRIMER_LEFT_0=55,22
PRIMER_RIGHT_0=197,20
PRIMER_INTERNAL_0=94,18
PRIMER_LEFT_0_TM=57.060
PRIMER_RIGHT_0_TM=59.592
PRIMER_INTERNAL_0_TM=62.453
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=143
PRIMER_PAIR_1_PENALTY=5.478193
PRIMER_LEFT_1_PENALTY=5.070252
PRIMER_RIGHT_1_PENALTY=0.407941
PRIMER_INTERNAL_1_PENALTY=4.452854
PRIMER_LEFT_1_SEQUENCE=GGTCACATAAAGAAAGGCACTAGAA
PRIMER_RIGHT_1_SEQUENCE=ACCTTGTGAAACCTGCCCTA
PRIMER_INTERNAL_1_SEQUENCE=TCTCCTCACCACCCGCTC
PRIMER_LEFT_1=56,25
PRIMER_RIGHT_1=197,20
PRIMER_INTERNAL_1=94,18
PRIMER_LEFT_1_TM=60.070
PRIMER_RIGHT_1_TM=59.592
PRIMER_INTERNAL_1_TM=62.453
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_2_PENALTY=5.518600
PRIMER_LEFT_2_PENALTY=5.110659
PRIMER_RIGHT_2_PENALTY=0.407941
PRIMER_INTERNAL_2_PENALTY=4.452854
PRIMER_LEFT_2_SEQUENCE=GGTCACATAAAGAAAGGCACTAGA
PRIMER_RIGHT_2_SEQUENCE=ACCTTGTGAAACCTGCCCTA
PRIMER_INTERNAL_2_SEQUENCE=TCTCCTCACCACCCGCTC
PRIMER_LEFT_2=56,24
PRIMER_RIGHT_2=197,20
PRIMER_INTERNAL_2=94,18
PRIMER_LEFT_2_TM=58.889
PRIMER_RIGHT_2_TM=59.592
PRIMER_INTERNAL_2_TM=62.453
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=66.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=142
PRIMER_PAIR_3_PENALTY=5.629249
PRIMER_LEFT_3_PENALTY=4.939568
PRIMER_RIGHT_3_PENALTY=0.689681
PRIMER_INTERNAL_3_PENALTY=4.452854
PRIMER_LEFT_3_SEQUENCE=AGGTCACATAAAGAAAGGCACT
PRIMER_RIGHT_3_SEQUENCE=CTGACCTTGTGAAACCTGCC
PRIMER_INTERNAL_3_SEQUENCE=TCTCCTCACCACCCGCTC
PRIMER_LEFT_3=55,22
PRIMER_RIGHT_3=200,20
PRIMER_INTERNAL_3=94,18
PRIMER_LEFT_3_TM=57.060
PRIMER_RIGHT_3_TM=60.690
PRIMER_INTERNAL_3_TM=62.453
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=66.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=146
PRIMER_PAIR_4_PENALTY=5.656863
PRIMER_LEFT_4_PENALTY=5.248922
PRIMER_RIGHT_4_PENALTY=0.407941
PRIMER_INTERNAL_4_PENALTY=4.452854
PRIMER_LEFT_4_SEQUENCE=AGGTCACATAAAGAAAGGCACTAGA
PRIMER_RIGHT_4_SEQUENCE=ACCTTGTGAAACCTGCCCTA
PRIMER_INTERNAL_4_SEQUENCE=TCTCCTCACCACCCGCTC
PRIMER_LEFT_4=55,25
PRIMER_RIGHT_4=197,20
PRIMER_INTERNAL_4=94,18
PRIMER_LEFT_4_TM=59.751
PRIMER_RIGHT_4_TM=59.592
PRIMER_INTERNAL_4_TM=62.453
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=143
=
SEQUENCE_ID=MH1195
SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGGGAGCACATGACAGATAACACACTGTCCCTGTTTGTCCCCATCCCTCAGCAGGCATGTTCAAGTTAGGGGCTGGGGGTGAGGAGCAACTNACACNNNTGATCCCAATGGAAGGACCACTGCACTCAGTGCTACAGGAGAGACATAGCAAGATAGGAAAGTGGGAGTGTGTGACTGCTTCTGGCTGGGGGATCAAAAATGGTTTAATGTAGATCTAATTTTTCCTGAGCACCACTTCTATTATCTCATTTAATCTTTATAACAACCATATGGGATAGATATTATCCCCATTTTACAGATGAGAAAACTGGGGCTCCAGAGTAGGGGT
SEQUENCE_INCLUDED_REGION=37,331
P3_COMMENT=CL is TGGGAGCACATGACAGATAA
P3_COMMENT=CR is GAGCCCCAGTTTTCTCATCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.549252
PRIMER_LEFT_0_PENALTY=0.420772
PRIMER_RIGHT_0_PENALTY=0.128480
PRIMER_INTERNAL_0_PENALTY=4.173280
PRIMER_LEFT_0_SEQUENCE=AATGGAAGGACCACTGCACT
PRIMER_RIGHT_0_SEQUENCE=GAAGCAGTCACACACTCCCA
PRIMER_INTERNAL_0_SEQUENCE=TGCTACAGGAGAGACATAGCAAG
PRIMER_LEFT_0=146,20
PRIMER_RIGHT_0=220,20
PRIMER_INTERNAL_0=169,23
PRIMER_LEFT_0_TM=59.579
PRIMER_RIGHT_0_TM=59.872
PRIMER_INTERNAL_0_TM=58.827
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.674645
PRIMER_LEFT_1_PENALTY=0.554836
PRIMER_RIGHT_1_PENALTY=0.119809
PRIMER_INTERNAL_1_PENALTY=3.604007
PRIMER_LEFT_1_SEQUENCE=TCAGCAGGCATGTTCAAGTT
PRIMER_RIGHT_1_SEQUENCE=GAGTGCAGTGGTCCTTCCAT
PRIMER_INTERNAL_1_SEQUENCE=CTGGGGGTGAGGAGCAACT
PRIMER_LEFT_1=87,20
PRIMER_RIGHT_1=166,20
PRIMER_INTERNAL_1=112,19
PRIMER_LEFT_1_TM=59.445
PRIMER_RIGHT_1_TM=60.120
PRIMER_INTERNAL_1_TM=62.604
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=63.158
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=0.703144
PRIMER_LEFT_2_PENALTY=0.574664
PRIMER_RIGHT_2_PENALTY=0.128480
PRIMER_INTERNAL_2_PENALTY=2.184188
PRIMER_LEFT_2_SEQUENCE=ATCCCAATGGAAGGACCACT
PRIMER_RIGHT_2_SEQUENCE=GAAGCAGTCACACACTCCCA
PRIMER_INTERNAL_2_SEQUENCE=GCACTCAGTGCTACAGGAGAGA
PRIMER_LEFT_2=141,20
PRIMER_RIGHT_2=220,20
PRIMER_INTERNAL_2=161,22
PRIMER_LEFT_2_TM=60.575
PRIMER_RIGHT_2_TM=59.872
PRIMER_INTERNAL_2_TM=59.816
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=9.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=0.715749
PRIMER_LEFT_3_PENALTY=0.141085
PRIMER_RIGHT_3_PENALTY=0.574664
PRIMER_INTERNAL_3_PENALTY=0.554836
PRIMER_LEFT_3_SEQUENCE=ACACTGTCCCTGTTTGTCCC
PRIMER_RIGHT_3_SEQUENCE=AGTGGTCCTTCCATTGGGAT
PRIMER_INTERNAL_3_SEQUENCE=TCAGCAGGCATGTTCAAGTT
PRIMER_LEFT_3=61,20
PRIMER_RIGHT_3=160,20
PRIMER_INTERNAL_3=87,20
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=60.575
PRIMER_INTERNAL_3_TM=59.445
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=0.923023
PRIMER_LEFT_4_PENALTY=0.121394
PRIMER_RIGHT_4_PENALTY=0.801629
PRIMER_INTERNAL_4_PENALTY=0.141085
PRIMER_LEFT_4_SEQUENCE=TGTGGGAGCACATGACAGAT
PRIMER_RIGHT_4_SEQUENCE=AGCCCCTAACTTGAACATGC
PRIMER_INTERNAL_4_SEQUENCE=ACACTGTCCCTGTTTGTCCC
PRIMER_LEFT_4=38,20
PRIMER_RIGHT_4=113,20
PRIMER_INTERNAL_4=61,20
PRIMER_LEFT_4_TM=60.121
PRIMER_RIGHT_4_TM=59.198
PRIMER_INTERNAL_4_TM=59.859
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1199
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTCTATTTCCCCAATTTCCTATAATTCAATAAAAAGTCAGCAATAACATGTCCAAGGGCATGTTATCTCAAATGTGGATTTTAATGAAACACAAATATGCCCATGCATGTTTTAGCAAATTGTTCTGGACCATGCAAAAATGCATTAAAAGTGGAGGCATATTTACATTTGTTTTGCATTTGTTATTTAAGAGGTCAGACTATGCAAAGTGCATCCACTTTTGTCAATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA
SEQUENCE_INCLUDED_REGION=36,231
P3_COMMENT=CL is CCTATAATTCAATAAAAAGTCAGCA
P3_COMMENT=CR is AAAGTGGATGCACTTTGCAT
P3_COMMENT=CM is TAACATGTCCAAGGGCATGT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.427417
PRIMER_LEFT_0_PENALTY=0.339170
PRIMER_RIGHT_0_PENALTY=0.088247
PRIMER_INTERNAL_0_PENALTY=0.184188
PRIMER_LEFT_0_SEQUENCE=GCAATAACATGTCCAAGGGC
PRIMER_RIGHT_0_SEQUENCE=TTTTGCATGGTCCAGAACAA
PRIMER_INTERNAL_0_SEQUENCE=AACACAAATATGCCCATGCA
PRIMER_LEFT_0=76,20
PRIMER_RIGHT_0=175,20
PRIMER_INTERNAL_0=124,20
PRIMER_LEFT_0_TM=60.339
PRIMER_RIGHT_0_TM=60.088
PRIMER_INTERNAL_0_TM=59.816
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.693006
PRIMER_LEFT_1_PENALTY=0.604759
PRIMER_RIGHT_1_PENALTY=0.088247
PRIMER_INTERNAL_1_PENALTY=0.184188
PRIMER_LEFT_1_SEQUENCE=CAATAACATGTCCAAGGGCA
PRIMER_RIGHT_1_SEQUENCE=TTTTGCATGGTCCAGAACAA
PRIMER_INTERNAL_1_SEQUENCE=AACACAAATATGCCCATGCA
PRIMER_LEFT_1=77,20
PRIMER_RIGHT_1=175,20
PRIMER_INTERNAL_1=124,20
PRIMER_LEFT_1_TM=59.395
PRIMER_RIGHT_1_TM=60.088
PRIMER_INTERNAL_1_TM=59.816
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=0.693006
PRIMER_LEFT_2_PENALTY=0.604759
PRIMER_RIGHT_2_PENALTY=0.088247
PRIMER_INTERNAL_2_PENALTY=0.184188
PRIMER_LEFT_2_SEQUENCE=CAATAACATGTCCAAGGGCA
PRIMER_RIGHT_2_SEQUENCE=TTTTTGCATGGTCCAGAACA
PRIMER_INTERNAL_2_SEQUENCE=AACACAAATATGCCCATGCA
PRIMER_LEFT_2=77,20
PRIMER_RIGHT_2=176,20
PRIMER_INTERNAL_2=124,20
PRIMER_LEFT_2_TM=59.395
PRIMER_RIGHT_2_TM=60.088
PRIMER_INTERNAL_2_TM=59.816
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=0.777578
PRIMER_LEFT_3_PENALTY=0.339170
PRIMER_RIGHT_3_PENALTY=0.438408
PRIMER_INTERNAL_3_PENALTY=5.031983
PRIMER_LEFT_3_SEQUENCE=GCAATAACATGTCCAAGGGC
PRIMER_RIGHT_3_SEQUENCE=TTTGCTAAAACATGCATGGG
PRIMER_INTERNAL_3_SEQUENCE=CAAATGTGGATTTTAATGAAACACA
PRIMER_LEFT_3=76,20
PRIMER_RIGHT_3=155,20
PRIMER_INTERNAL_3=105,25
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=59.562
PRIMER_INTERNAL_3_TM=60.032
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=28.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=1.013002
PRIMER_LEFT_4_PENALTY=0.924755
PRIMER_RIGHT_4_PENALTY=0.088247
PRIMER_INTERNAL_4_PENALTY=0.184188
PRIMER_LEFT_4_SEQUENCE=TCCAAGGGCATGTTATCTCA
PRIMER_RIGHT_4_SEQUENCE=TTTTGCATGGTCCAGAACAA
PRIMER_INTERNAL_4_SEQUENCE=AACACAAATATGCCCATGCA
PRIMER_LEFT_4=87,20
PRIMER_RIGHT_4=175,20
PRIMER_INTERNAL_4=124,20
PRIMER_LEFT_4_TM=59.075
PRIMER_RIGHT_4_TM=60.088
PRIMER_INTERNAL_4_TM=59.816
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH1200
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATGTTAACATGCTGTGACCAGTAAACCAAAGATGCAGTGATTGCTAGTAGGCTCCATCGTCGGCCCCTTGCAGGTGGTGGGGACATATTCAGGGGTGGTGGATATCAGTGAGCACAGCCCCTGACTGGTGGCTGCCCATGCAGAGTGTGGGGGTGGGGAGTAGATGGGGGGCTTTTCCTAATGCATCTAGCACCCAGTGTCTCTGGCTTCCTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC
SEQUENCE_INCLUDED_REGION=36,218
P3_COMMENT=CL is TGCTGTGACCAGTAAACCAA
P3_COMMENT=CR is TAGGAAGCCAGAGACACTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.103650
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.103613
PRIMER_INTERNAL_0_PENALTY=0.049859
PRIMER_LEFT_0_SEQUENCE=GCTAGTAGGCTCCATCGTCG
PRIMER_RIGHT_0_SEQUENCE=GGGGCTGTGCTCACTGATAT
PRIMER_INTERNAL_0_SEQUENCE=GACATATTCAGGGGTGGTGG
PRIMER_LEFT_0=82,20
PRIMER_RIGHT_0=160,20
PRIMER_INTERNAL_0=121,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=60.104
PRIMER_INTERNAL_0_TM=60.050
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.1000
PRIMER_RIGHT_0_END_STABILITY=5.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=79
PRIMER_PAIR_1_PENALTY=0.146679
PRIMER_LEFT_1_PENALTY=0.049859
PRIMER_RIGHT_1_PENALTY=0.096820
PRIMER_INTERNAL_1_PENALTY=0.822509
PRIMER_LEFT_1_SEQUENCE=ACATATTCAGGGGTGGTGGA
PRIMER_RIGHT_1_SEQUENCE=GAAAAGCCCCCCATCTACTC
PRIMER_INTERNAL_1_SEQUENCE=TATCAGTGAGCACAGCCCCT
PRIMER_LEFT_1=122,20
PRIMER_RIGHT_1=216,20
PRIMER_INTERNAL_1=142,20
PRIMER_LEFT_1_TM=60.050
PRIMER_RIGHT_1_TM=59.903
PRIMER_INTERNAL_1_TM=60.823
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=0.146679
PRIMER_LEFT_2_PENALTY=0.049859
PRIMER_RIGHT_2_PENALTY=0.096820
PRIMER_INTERNAL_2_PENALTY=0.103613
PRIMER_LEFT_2_SEQUENCE=GACATATTCAGGGGTGGTGG
PRIMER_RIGHT_2_SEQUENCE=GAAAAGCCCCCCATCTACTC
PRIMER_INTERNAL_2_SEQUENCE=ATATCAGTGAGCACAGCCCC
PRIMER_LEFT_2=121,20
PRIMER_RIGHT_2=216,20
PRIMER_INTERNAL_2=141,20
PRIMER_LEFT_2_TM=60.050
PRIMER_RIGHT_2_TM=59.903
PRIMER_INTERNAL_2_TM=60.104
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.146679
PRIMER_LEFT_3_PENALTY=0.049859
PRIMER_RIGHT_3_PENALTY=0.096820
PRIMER_INTERNAL_3_PENALTY=0.103613
PRIMER_LEFT_3_SEQUENCE=GGACATATTCAGGGGTGGTG
PRIMER_RIGHT_3_SEQUENCE=GAAAAGCCCCCCATCTACTC
PRIMER_INTERNAL_3_SEQUENCE=ATATCAGTGAGCACAGCCCC
PRIMER_LEFT_3=120,20
PRIMER_RIGHT_3=216,20
PRIMER_INTERNAL_3=141,20
PRIMER_LEFT_3_TM=60.050
PRIMER_RIGHT_3_TM=59.903
PRIMER_INTERNAL_3_TM=60.104
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=0.200433
PRIMER_LEFT_4_PENALTY=0.103613
PRIMER_RIGHT_4_PENALTY=0.096820
PRIMER_INTERNAL_4_PENALTY=2.027014
PRIMER_LEFT_4_SEQUENCE=ATATCAGTGAGCACAGCCCC
PRIMER_RIGHT_4_SEQUENCE=GAAAAGCCCCCCATCTACTC
PRIMER_INTERNAL_4_SEQUENCE=CTGCCCATGCAGAGTGTG
PRIMER_LEFT_4=141,20
PRIMER_RIGHT_4=216,20
PRIMER_INTERNAL_4=171,18
PRIMER_LEFT_4_TM=60.104
PRIMER_RIGHT_4_TM=59.903
PRIMER_INTERNAL_4_TM=59.973
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1203
SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCACATCCACTGCTTACTTACAAAAGAAAGTACAAACTTAGAGAAGGCCTTCCATGGTTTGGTTGCTACTGACTTTGTTAGCCTTACTGCCCACTATGCATTGGAACATTCCCATATTCCAACTAAGCAGGAGTGTTCACAATAAACAACATAGGCTCTTTATTCTCCTTCTTTCATTAATTTTCTTTCACGTTATTCCCTCACCCTGAACGCCCTTCTTCCTTCGTAGTGACATTTTAAAATCCACTTTACACATTCGGACCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
SEQUENCE_INCLUDED_REGION=36,268
P3_COMMENT=CL is TCCATGGTTTGGTTGCTACT
P3_COMMENT=CR is GGTCCGAATGTGTAAAGTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.094669
PRIMER_LEFT_0_PENALTY=0.073866
PRIMER_RIGHT_0_PENALTY=0.020803
PRIMER_INTERNAL_0_PENALTY=1.561873
PRIMER_LEFT_0_SEQUENCE=AGAGAAGGCCTTCCATGGTT
PRIMER_RIGHT_0_SEQUENCE=ATGGGAATGTTCCAATGCAT
PRIMER_INTERNAL_0_SEQUENCE=TTTGTTAGCCTTACTGCCCA
PRIMER_LEFT_0=79,20
PRIMER_RIGHT_0=154,20
PRIMER_INTERNAL_0=113,20
PRIMER_LEFT_0_TM=60.074
PRIMER_RIGHT_0_TM=60.021
PRIMER_INTERNAL_0_TM=58.438
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=10.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=0.328864
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.254998
PRIMER_INTERNAL_1_PENALTY=1.561873
PRIMER_LEFT_1_SEQUENCE=AGAGAAGGCCTTCCATGGTT
PRIMER_RIGHT_1_SEQUENCE=TGGGAATGTTCCAATGCATA
PRIMER_INTERNAL_1_SEQUENCE=TTTGTTAGCCTTACTGCCCA
PRIMER_LEFT_1=79,20
PRIMER_RIGHT_1=153,20
PRIMER_INTERNAL_1=113,20
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.745
PRIMER_INTERNAL_1_TM=58.438
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=0.328864
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.254998
PRIMER_INTERNAL_2_PENALTY=1.561873
PRIMER_LEFT_2_SEQUENCE=AGAGAAGGCCTTCCATGGTT
PRIMER_RIGHT_2_SEQUENCE=TATGGGAATGTTCCAATGCA
PRIMER_INTERNAL_2_SEQUENCE=TTTGTTAGCCTTACTGCCCA
PRIMER_LEFT_2=79,20
PRIMER_RIGHT_2=155,20
PRIMER_INTERNAL_2=113,20
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.745
PRIMER_INTERNAL_2_TM=58.438
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=0.463701
PRIMER_LEFT_3_PENALTY=0.442898
PRIMER_RIGHT_3_PENALTY=0.020803
PRIMER_INTERNAL_3_PENALTY=1.561873
PRIMER_LEFT_3_SEQUENCE=GAGAAGGCCTTCCATGGTTT
PRIMER_RIGHT_3_SEQUENCE=ATGGGAATGTTCCAATGCAT
PRIMER_INTERNAL_3_SEQUENCE=TTTGTTAGCCTTACTGCCCA
PRIMER_LEFT_3=80,20
PRIMER_RIGHT_3=154,20
PRIMER_INTERNAL_3=113,20
PRIMER_LEFT_3_TM=60.443
PRIMER_RIGHT_3_TM=60.021
PRIMER_INTERNAL_3_TM=58.438
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=0.697896
PRIMER_LEFT_4_PENALTY=0.442898
PRIMER_RIGHT_4_PENALTY=0.254998
PRIMER_INTERNAL_4_PENALTY=1.561873
PRIMER_LEFT_4_SEQUENCE=GAGAAGGCCTTCCATGGTTT
PRIMER_RIGHT_4_SEQUENCE=TATGGGAATGTTCCAATGCA
PRIMER_INTERNAL_4_SEQUENCE=TTTGTTAGCCTTACTGCCCA
PRIMER_LEFT_4=80,20
PRIMER_RIGHT_4=155,20
PRIMER_INTERNAL_4=113,20
PRIMER_LEFT_4_TM=60.443
PRIMER_RIGHT_4_TM=59.745
PRIMER_INTERNAL_4_TM=58.438
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=10.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1207
SEQUENCE_TEMPLATE=TNGCATTGCCTNCAGGTCGACTCTAGAGGATCCCGATGTGCTGCAAGGCGATTAAGTTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG
SEQUENCE_INCLUDED_REGION=34,24
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1208
SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTGCCTTTGAGATTTGTNCAAGTTATTGCTCATTATCTAGTTTGTNTCTTTTTATTGCCCTTGTCTTCTGTTTCATGGGTGTGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTC
SEQUENCE_INCLUDED_REGION=35,81
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH1214
SEQUENCE_TEMPLATE=NTGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTTCTTTGAATGTGCATGTGTGTGTGTGTGTCCTGGATGGGATCCTACTGCTATCTCTTCTTTTTCTCACCCAGTGACATGCCTTTCTATGCAGATCCATCTGGATCTNNTNTAGTTTTGCTCACTGCTGCGTAGCTTTCATGGTATGAATGCCAGAAGCCATTTAGCACCTCCCATAATGAGGGGATTTATGTTTTTNCCAGACCAGTGGTCTACAGATTGAGGGGTACNGAGNCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGA
SEQUENCE_INCLUDED_REGION=38,226
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.228568
PRIMER_LEFT_0_PENALTY=0.202638
PRIMER_RIGHT_0_PENALTY=0.025930
PRIMER_INTERNAL_0_PENALTY=0.466916
PRIMER_LEFT_0_SEQUENCE=GTTTTGCTCACTGCTGCGTA
PRIMER_RIGHT_0_SEQUENCE=AATCCCCTCATTATGGGAGG
PRIMER_INTERNAL_0_SEQUENCE=ATGAATGCCAGAAGCCATTT
PRIMER_LEFT_0=154,20
PRIMER_RIGHT_0=228,20
PRIMER_INTERNAL_0=185,20
PRIMER_LEFT_0_TM=60.203
PRIMER_RIGHT_0_TM=59.974
PRIMER_INTERNAL_0_TM=59.533
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=7.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.363749
PRIMER_LEFT_1_PENALTY=0.337818
PRIMER_RIGHT_1_PENALTY=0.025930
PRIMER_INTERNAL_1_PENALTY=0.466916
PRIMER_LEFT_1_SEQUENCE=TAGTTTTGCTCACTGCTGCG
PRIMER_RIGHT_1_SEQUENCE=AATCCCCTCATTATGGGAGG
PRIMER_INTERNAL_1_SEQUENCE=ATGAATGCCAGAAGCCATTT
PRIMER_LEFT_1=152,20
PRIMER_RIGHT_1=228,20
PRIMER_INTERNAL_1=185,20
PRIMER_LEFT_1_TM=60.338
PRIMER_RIGHT_1_TM=59.974
PRIMER_INTERNAL_1_TM=59.533
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=0.702067
PRIMER_LEFT_2_PENALTY=0.624727
PRIMER_RIGHT_2_PENALTY=0.077340
PRIMER_INTERNAL_2_PENALTY=0.202638
PRIMER_LEFT_2_SEQUENCE=TGCCTTTCTATGCAGATCCA
PRIMER_RIGHT_2_SEQUENCE=TATGGGAGGTGCTAAATGGC
PRIMER_INTERNAL_2_SEQUENCE=GTTTTGCTCACTGCTGCGTA
PRIMER_LEFT_2=119,20
PRIMER_RIGHT_2=217,20
PRIMER_INTERNAL_2=154,20
PRIMER_LEFT_2_TM=59.375
PRIMER_RIGHT_2_TM=59.923
PRIMER_INTERNAL_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=0.712421
PRIMER_LEFT_3_PENALTY=0.087694
PRIMER_RIGHT_3_PENALTY=0.624727
PRIMER_INTERNAL_3_PENALTY=2.248781
PRIMER_LEFT_3_SEQUENCE=TGTGTGTGTGTGTGTCCTGG
PRIMER_RIGHT_3_SEQUENCE=TGGATCTGCATAGAAAGGCA
PRIMER_INTERNAL_3_SEQUENCE=TTCTTTTTCTCACCCAGTGACA
PRIMER_LEFT_3=56,20
PRIMER_RIGHT_3=138,20
PRIMER_INTERNAL_3=97,22
PRIMER_LEFT_3_TM=60.088
PRIMER_RIGHT_3_TM=59.375
PRIMER_INTERNAL_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=0.797136
PRIMER_LEFT_4_PENALTY=0.376364
PRIMER_RIGHT_4_PENALTY=0.420772
PRIMER_INTERNAL_4_PENALTY=0.120594
PRIMER_LEFT_4_SEQUENCE=TGTGCATGTGTGTGTGTGTG
PRIMER_RIGHT_4_SEQUENCE=AGAAAGGCATGTCACTGGGT
PRIMER_INTERNAL_4_SEQUENCE=TCCTGGATGGGATCCTACTG
PRIMER_LEFT_4=50,20
PRIMER_RIGHT_4=127,20
PRIMER_INTERNAL_4=70,20
PRIMER_LEFT_4_TM=59.624
PRIMER_RIGHT_4_TM=59.579
PRIMER_INTERNAL_4_TM=59.879
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH1215
SEQUENCE_TEMPLATE=TTGCANNGCCTNCAGGTCGACTCTAGAGGATCCCCCTAGGCAGAGNNNTATCTCATTTTCTTTCATGGTTTAGGTNAAGTNACGTTAACTGCAGGCAGANGGCTAGTTTATAGTTTGAACAGACTCAAAATTNNTTTANACAGGACAAAGAAACAACAGAAGTACATATTATAAANCTTTTAAACTNTTCCCTAATATAACACTTCNTTTCCACTTGATACTGAAAATTTTTAAANTTCTCAACCTTAATTCAATTTACATCTATTTAAAAACATTATATAANACATTCTATATTANCAATGAATTGATTTGACTGTTTTC
SEQUENCE_INCLUDED_REGION=35,286
P3_COMMENT=CL is CTCATTTTCTTTCATGGTTTAGG
P3_COMMENT=CR is AAAACAGTCAAATCAATTCATTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=10.675442
PRIMER_LEFT_0_PENALTY=5.264677
PRIMER_RIGHT_0_PENALTY=5.410764
PRIMER_INTERNAL_0_PENALTY=8.349211
PRIMER_LEFT_0_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG
PRIMER_RIGHT_0_SEQUENCE=CTTCTGTTGTTTCTTTGTCCTGT
PRIMER_INTERNAL_0_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
PRIMER_LEFT_0=50,24
PRIMER_RIGHT_0=161,23
PRIMER_INTERNAL_0=100,27
PRIMER_LEFT_0_TM=58.735
PRIMER_RIGHT_0_TM=57.589
PRIMER_INTERNAL_0_TM=58.651
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_INTERNAL_0_GC_PERCENT=37.037
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.5000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=112
PRIMER_PAIR_1_PENALTY=10.675442
PRIMER_LEFT_1_PENALTY=5.264677
PRIMER_RIGHT_1_PENALTY=5.410764
PRIMER_INTERNAL_1_PENALTY=8.349211
PRIMER_LEFT_1_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG
PRIMER_RIGHT_1_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTG
PRIMER_INTERNAL_1_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
PRIMER_LEFT_1=50,24
PRIMER_RIGHT_1=162,23
PRIMER_INTERNAL_1=100,27
PRIMER_LEFT_1_TM=58.735
PRIMER_RIGHT_1_TM=57.589
PRIMER_INTERNAL_1_TM=58.651
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_INTERNAL_1_GC_PERCENT=37.037
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=113
PRIMER_PAIR_2_PENALTY=10.831436
PRIMER_LEFT_2_PENALTY=5.264677
PRIMER_RIGHT_2_PENALTY=5.566758
PRIMER_INTERNAL_2_PENALTY=8.349211
PRIMER_LEFT_2_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG
PRIMER_RIGHT_2_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTGT
PRIMER_INTERNAL_2_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
PRIMER_LEFT_2=50,24
PRIMER_RIGHT_2=162,24
PRIMER_INTERNAL_2=100,27
PRIMER_LEFT_2_TM=58.735
PRIMER_RIGHT_2_TM=58.433
PRIMER_INTERNAL_2_TM=58.651
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=37.500
PRIMER_INTERNAL_2_GC_PERCENT=37.037
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=113
PRIMER_PAIR_3_PENALTY=10.939152
PRIMER_LEFT_3_PENALTY=5.528388
PRIMER_RIGHT_3_PENALTY=5.410764
PRIMER_INTERNAL_3_PENALTY=8.349211
PRIMER_LEFT_3_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGGT
PRIMER_RIGHT_3_SEQUENCE=CTTCTGTTGTTTCTTTGTCCTGT
PRIMER_INTERNAL_3_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
PRIMER_LEFT_3=50,25
PRIMER_RIGHT_3=161,23
PRIMER_INTERNAL_3=100,27
PRIMER_LEFT_3_TM=59.472
PRIMER_RIGHT_3_TM=57.589
PRIMER_INTERNAL_3_TM=58.651
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_INTERNAL_3_GC_PERCENT=37.037
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=112
PRIMER_PAIR_4_PENALTY=10.939152
PRIMER_LEFT_4_PENALTY=5.528388
PRIMER_RIGHT_4_PENALTY=5.410764
PRIMER_INTERNAL_4_PENALTY=8.349211
PRIMER_LEFT_4_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGGT
PRIMER_RIGHT_4_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTG
PRIMER_INTERNAL_4_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA
PRIMER_LEFT_4=50,25
PRIMER_RIGHT_4=162,23
PRIMER_INTERNAL_4=100,27
PRIMER_LEFT_4_TM=59.472
PRIMER_RIGHT_4_TM=57.589
PRIMER_INTERNAL_4_TM=58.651
PRIMER_LEFT_4_GC_PERCENT=32.000
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_INTERNAL_4_GC_PERCENT=37.037
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=113
=
SEQUENCE_ID=MH1216
SEQUENCE_TEMPLATE=GCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCAGGGTNNTGACCTGTGGAGTCTCAACCCTAACAGCACATTAGCACCACTTGGGGAGCTTCTGAAAAATACTGGTACCCGGGGCCTTAGCATAGGTATTGTTCAAAACCTCTCAAGGTGATTTAAATGAGTAACAAGGGTTGAAAAACCNTGATTTTGGCAGCACAAACCAAAAGAGCAGGCAGGGCCGNGAGAGGGAAGTCAGTGGTACCAGAAGGTAGATGGGCTCCCTTGCAGGCTCCTTGTTCTCCTGCCATCACCAGTAGAACCTTCTGGCTGACAGACCAGGGACAAGTAGACTGGGTTCAAAGTG
SEQUENCE_INCLUDED_REGION=32,314
P3_COMMENT=CL is TGACCTGTGGAGTCTCAACC
P3_COMMENT=CR is TTGAACCCAGTCTACTTGTCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.160863
PRIMER_LEFT_0_PENALTY=0.027122
PRIMER_RIGHT_0_PENALTY=0.133741
PRIMER_INTERNAL_0_PENALTY=0.979027
PRIMER_LEFT_0_SEQUENCE=CAGCACAAACCAAAAGAGCA
PRIMER_RIGHT_0_SEQUENCE=GAGAACAAGGAGCCTGCAAG
PRIMER_INTERNAL_0_SEQUENCE=CCAGAAGGTAGATGGGCTCC
PRIMER_LEFT_0=193,20
PRIMER_RIGHT_0=282,20
PRIMER_INTERNAL_0=243,20
PRIMER_LEFT_0_TM=60.027
PRIMER_RIGHT_0_TM=60.134
PRIMER_INTERNAL_0_TM=60.979
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=0.224025
PRIMER_LEFT_1_PENALTY=0.027122
PRIMER_RIGHT_1_PENALTY=0.196903
PRIMER_INTERNAL_1_PENALTY=0.455083
PRIMER_LEFT_1_SEQUENCE=CAGCACAAACCAAAAGAGCA
PRIMER_RIGHT_1_SEQUENCE=GATGGCAGGAGAACAAGGAG
PRIMER_INTERNAL_1_SEQUENCE=AAGGTAGATGGGCTCCCTTG
PRIMER_LEFT_1=193,20
PRIMER_RIGHT_1=290,20
PRIMER_INTERNAL_1=247,20
PRIMER_LEFT_1_TM=60.027
PRIMER_RIGHT_1_TM=59.803
PRIMER_INTERNAL_1_TM=60.455
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=98
PRIMER_PAIR_2_PENALTY=0.683515
PRIMER_LEFT_2_PENALTY=0.027122
PRIMER_RIGHT_2_PENALTY=0.656393
PRIMER_INTERNAL_2_PENALTY=0.455083
PRIMER_LEFT_2_SEQUENCE=CAGCACAAACCAAAAGAGCA
PRIMER_RIGHT_2_SEQUENCE=GTGATGGCAGGAGAACAAGG
PRIMER_INTERNAL_2_SEQUENCE=AAGGTAGATGGGCTCCCTTG
PRIMER_LEFT_2=193,20
PRIMER_RIGHT_2=292,20
PRIMER_INTERNAL_2=247,20
PRIMER_LEFT_2_TM=60.027
PRIMER_RIGHT_2_TM=60.656
PRIMER_INTERNAL_2_TM=60.455
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=0.778219
PRIMER_LEFT_3_PENALTY=0.466041
PRIMER_RIGHT_3_PENALTY=0.312177
PRIMER_INTERNAL_3_PENALTY=0.366450
PRIMER_LEFT_3_SEQUENCE=GACCTGTGGAGTCTCAACCC
PRIMER_RIGHT_3_SEQUENCE=ATACCTATGCTAAGGCCCCG
PRIMER_INTERNAL_3_SEQUENCE=CACTTGGGGAGCTTCTGAAA
PRIMER_LEFT_3=43,20
PRIMER_RIGHT_3=131,20
PRIMER_INTERNAL_3=80,20
PRIMER_LEFT_3_TM=59.534
PRIMER_RIGHT_3_TM=60.312
PRIMER_INTERNAL_3_TM=60.366
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=0.887023
PRIMER_LEFT_4_PENALTY=0.455083
PRIMER_RIGHT_4_PENALTY=0.431941
PRIMER_INTERNAL_4_PENALTY=0.119809
PRIMER_LEFT_4_SEQUENCE=AAGGTAGATGGGCTCCCTTG
PRIMER_RIGHT_4_SEQUENCE=CCCAGTCTACTTGTCCCTGG
PRIMER_INTERNAL_4_SEQUENCE=GTTCTCCTGCCATCACCAGT
PRIMER_LEFT_4=247,20
PRIMER_RIGHT_4=336,20
PRIMER_INTERNAL_4=277,20
PRIMER_LEFT_4_TM=60.455
PRIMER_RIGHT_4_TM=59.568
PRIMER_INTERNAL_4_TM=60.120
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH1217
SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGAAGACCAAAAAGACAGACCTCCCTTTCTTACTGAGAAGAGCGTAGTCAGACATGTCAAGTCTGGCACGCTGCTGCAGAAGGCGTGATTCATCTTAAGATCAAAAATTATTTCATCTTGCTTTCTTNNTCATAAGGGTACCGNGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATNCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGC
SEQUENCE_INCLUDED_REGION=37,137
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.405698
PRIMER_LEFT_0_PENALTY=1.035219
PRIMER_RIGHT_0_PENALTY=0.370479
PRIMER_INTERNAL_0_PENALTY=0.318581
PRIMER_LEFT_0_SEQUENCE=GACCAAAAAGACAGACCTCCC
PRIMER_RIGHT_0_SEQUENCE=AGATGAATCACGCCTTCTGC
PRIMER_INTERNAL_0_SEQUENCE=GACATGTCAAGTCTGGCACG
PRIMER_LEFT_0=43,21
PRIMER_RIGHT_0=133,20
PRIMER_INTERNAL_0=89,20
PRIMER_LEFT_0_TM=59.965
PRIMER_RIGHT_0_TM=60.370
PRIMER_INTERNAL_0_TM=60.319
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=1.673804
PRIMER_LEFT_1_PENALTY=1.303324
PRIMER_RIGHT_1_PENALTY=0.370479
PRIMER_INTERNAL_1_PENALTY=0.318581
PRIMER_LEFT_1_SEQUENCE=TGGGAAGACCAAAAAGACAGA
PRIMER_RIGHT_1_SEQUENCE=AGATGAATCACGCCTTCTGC
PRIMER_INTERNAL_1_SEQUENCE=GACATGTCAAGTCTGGCACG
PRIMER_LEFT_1=37,21
PRIMER_RIGHT_1=133,20
PRIMER_INTERNAL_1=89,20
PRIMER_LEFT_1_TM=59.697
PRIMER_RIGHT_1_TM=60.370
PRIMER_INTERNAL_1_TM=60.319
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=1.768499
PRIMER_LEFT_2_PENALTY=1.398020
PRIMER_RIGHT_2_PENALTY=0.370479
PRIMER_INTERNAL_2_PENALTY=0.318581
PRIMER_LEFT_2_SEQUENCE=CCAAAAAGACAGACCTCCCTT
PRIMER_RIGHT_2_SEQUENCE=AGATGAATCACGCCTTCTGC
PRIMER_INTERNAL_2_SEQUENCE=GACATGTCAAGTCTGGCACG
PRIMER_LEFT_2=45,21
PRIMER_RIGHT_2=133,20
PRIMER_INTERNAL_2=89,20
PRIMER_LEFT_2_TM=59.602
PRIMER_RIGHT_2_TM=60.370
PRIMER_INTERNAL_2_TM=60.319
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=2.142615
PRIMER_LEFT_3_PENALTY=1.772136
PRIMER_RIGHT_3_PENALTY=0.370479
PRIMER_INTERNAL_3_PENALTY=0.318581
PRIMER_LEFT_3_SEQUENCE=CCAAAAAGACAGACCTCCCT
PRIMER_RIGHT_3_SEQUENCE=AGATGAATCACGCCTTCTGC
PRIMER_INTERNAL_3_SEQUENCE=GACATGTCAAGTCTGGCACG
PRIMER_LEFT_3=45,20
PRIMER_RIGHT_3=133,20
PRIMER_INTERNAL_3=89,20
PRIMER_LEFT_3_TM=58.228
PRIMER_RIGHT_3_TM=60.370
PRIMER_INTERNAL_3_TM=60.319
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=2.269568
PRIMER_LEFT_4_PENALTY=1.899089
PRIMER_RIGHT_4_PENALTY=0.370479
PRIMER_INTERNAL_4_PENALTY=0.318581
PRIMER_LEFT_4_SEQUENCE=ACCAAAAAGACAGACCTCCCT
PRIMER_RIGHT_4_SEQUENCE=AGATGAATCACGCCTTCTGC
PRIMER_INTERNAL_4_SEQUENCE=GACATGTCAAGTCTGGCACG
PRIMER_LEFT_4=44,21
PRIMER_RIGHT_4=133,20
PRIMER_INTERNAL_4=89,20
PRIMER_LEFT_4_TM=59.101
PRIMER_RIGHT_4_TM=60.370
PRIMER_INTERNAL_4_TM=60.319
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH1218
SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGACTACAGGGATCTCTTAAGCNCGGGAGGNGAGGNNAACGTGGGCTGTGATCGCACACTTCCACTCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTNGGGAAACCTGTCGTGCCAGCTGCATTAATG
SEQUENCE_INCLUDED_REGION=33,71
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1221
SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTAAATGTNNCCTCTTCAGAAAAGCCCTCTCTGACCATCTGCTCAGAAGCAGTCCCATCCAGTGACTCTCCTTTGCTGTTATCACCACTTGAAGTTTGCAATTAGTGCATTTCTATGTGTACCTCCTGCTTTCCCCATTAGAATGTGTCTTAGTTCATGTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGNNNGCTAACTCACATTAATTGCGTTGCGCTCACTGC
SEQUENCE_INCLUDED_REGION=37,164
P3_COMMENT=CL is CCTCTTCAGAAAAGCCCTCT
P3_COMMENT=CR is ATTCTAATGGGGAAAGCAGG
P3_COMMENT=CM is TGACCATCTGCTCAGAAGCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.230886
PRIMER_LEFT_0_PENALTY=0.119809
PRIMER_RIGHT_0_PENALTY=0.111077
PRIMER_INTERNAL_0_PENALTY=2.132661
PRIMER_LEFT_0_SEQUENCE=AAGCAGTCCCATCCAGTGAC
PRIMER_RIGHT_0_SEQUENCE=GGGAAAGCAGGAGGTACACA
PRIMER_INTERNAL_0_SEQUENCE=TCACCACTTGAAGTTTGCAA
PRIMER_LEFT_0=84,20
PRIMER_RIGHT_0=172,20
PRIMER_INTERNAL_0=119,20
PRIMER_LEFT_0_TM=60.120
PRIMER_RIGHT_0_TM=60.111
PRIMER_INTERNAL_0_TM=57.867
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=0.371834
PRIMER_LEFT_1_PENALTY=0.260757
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_INTERNAL_1_PENALTY=2.132661
PRIMER_LEFT_1_SEQUENCE=AGAAGCAGTCCCATCCAGTG
PRIMER_RIGHT_1_SEQUENCE=GGGAAAGCAGGAGGTACACA
PRIMER_INTERNAL_1_SEQUENCE=TCACCACTTGAAGTTTGCAA
PRIMER_LEFT_1=82,20
PRIMER_RIGHT_1=172,20
PRIMER_INTERNAL_1=119,20
PRIMER_LEFT_1_TM=60.261
PRIMER_RIGHT_1_TM=60.111
PRIMER_INTERNAL_1_TM=57.867
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.371834
PRIMER_LEFT_2_PENALTY=0.260757
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_INTERNAL_2_PENALTY=2.132661
PRIMER_LEFT_2_SEQUENCE=CAGAAGCAGTCCCATCCAGT
PRIMER_RIGHT_2_SEQUENCE=GGGAAAGCAGGAGGTACACA
PRIMER_INTERNAL_2_SEQUENCE=TCACCACTTGAAGTTTGCAA
PRIMER_LEFT_2=81,20
PRIMER_RIGHT_2=172,20
PRIMER_INTERNAL_2=119,20
PRIMER_LEFT_2_TM=60.261
PRIMER_RIGHT_2_TM=60.111
PRIMER_INTERNAL_2_TM=57.867
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=0.397701
PRIMER_LEFT_3_PENALTY=0.286624
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_INTERNAL_3_PENALTY=2.132661
PRIMER_LEFT_3_SEQUENCE=AGCAGTCCCATCCAGTGACT
PRIMER_RIGHT_3_SEQUENCE=GGGAAAGCAGGAGGTACACA
PRIMER_INTERNAL_3_SEQUENCE=TCACCACTTGAAGTTTGCAA
PRIMER_LEFT_3=85,20
PRIMER_RIGHT_3=172,20
PRIMER_INTERNAL_3=119,20
PRIMER_LEFT_3_TM=59.713
PRIMER_RIGHT_3_TM=60.111
PRIMER_INTERNAL_3_TM=57.867
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=0.447343
PRIMER_LEFT_4_PENALTY=0.119809
PRIMER_RIGHT_4_PENALTY=0.327533
PRIMER_INTERNAL_4_PENALTY=2.132661
PRIMER_LEFT_4_SEQUENCE=AAGCAGTCCCATCCAGTGAC
PRIMER_RIGHT_4_SEQUENCE=ATGGGGAAAGCAGGAGGTAC
PRIMER_INTERNAL_4_SEQUENCE=TCACCACTTGAAGTTTGCAA
PRIMER_LEFT_4=84,20
PRIMER_RIGHT_4=175,20
PRIMER_INTERNAL_4=119,20
PRIMER_LEFT_4_TM=60.120
PRIMER_RIGHT_4_TM=60.328
PRIMER_INTERNAL_4_TM=57.867
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH1225
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATAGAAAGCATTGTCTATCTGTAACCTAACCTAGTNCCTCTAACACTTCCTTGCACTTGTTGTTTACAAGGCTGCTTCCCCATTAGACTACGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTNCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
SEQUENCE_INCLUDED_REGION=36,97
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.426139
PRIMER_LEFT_0_PENALTY=7.827404
PRIMER_RIGHT_0_PENALTY=0.598735
PRIMER_INTERNAL_0_PENALTY=2.671989
PRIMER_LEFT_0_SEQUENCE=GCATTGTCTATCTGTAACCTAACCT
PRIMER_RIGHT_0_SEQUENCE=GTCTAATGGGGAAGCAGCCT
PRIMER_INTERNAL_0_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
PRIMER_LEFT_0=46,25
PRIMER_RIGHT_0=126,20
PRIMER_INTERNAL_0=82,22
PRIMER_LEFT_0_TM=57.173
PRIMER_RIGHT_0_TM=60.599
PRIMER_INTERNAL_0_TM=59.328
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=8.426139
PRIMER_LEFT_1_PENALTY=7.827404
PRIMER_RIGHT_1_PENALTY=0.598735
PRIMER_INTERNAL_1_PENALTY=2.671989
PRIMER_LEFT_1_SEQUENCE=GCATTGTCTATCTGTAACCTAACCT
PRIMER_RIGHT_1_SEQUENCE=AGTCTAATGGGGAAGCAGCC
PRIMER_INTERNAL_1_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
PRIMER_LEFT_1=46,25
PRIMER_RIGHT_1=127,20
PRIMER_INTERNAL_1=82,22
PRIMER_LEFT_1_TM=57.173
PRIMER_RIGHT_1_TM=60.599
PRIMER_INTERNAL_1_TM=59.328
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=8.426139
PRIMER_LEFT_2_PENALTY=7.827404
PRIMER_RIGHT_2_PENALTY=0.598735
PRIMER_INTERNAL_2_PENALTY=2.671989
PRIMER_LEFT_2_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC
PRIMER_RIGHT_2_SEQUENCE=GTCTAATGGGGAAGCAGCCT
PRIMER_INTERNAL_2_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
PRIMER_LEFT_2=45,25
PRIMER_RIGHT_2=126,20
PRIMER_INTERNAL_2=82,22
PRIMER_LEFT_2_TM=57.173
PRIMER_RIGHT_2_TM=60.599
PRIMER_INTERNAL_2_TM=59.328
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=8.426139
PRIMER_LEFT_3_PENALTY=7.827404
PRIMER_RIGHT_3_PENALTY=0.598735
PRIMER_INTERNAL_3_PENALTY=2.671989
PRIMER_LEFT_3_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC
PRIMER_RIGHT_3_SEQUENCE=AGTCTAATGGGGAAGCAGCC
PRIMER_INTERNAL_3_SEQUENCE=CACTTCCTTGCACTTGTTGTTT
PRIMER_LEFT_3=45,25
PRIMER_RIGHT_3=127,20
PRIMER_INTERNAL_3=82,22
PRIMER_LEFT_3_TM=57.173
PRIMER_RIGHT_3_TM=60.599
PRIMER_INTERNAL_3_TM=59.328
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=8.814430
PRIMER_LEFT_4_PENALTY=7.827404
PRIMER_RIGHT_4_PENALTY=0.987026
PRIMER_INTERNAL_4_PENALTY=3.333513
PRIMER_LEFT_4_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC
PRIMER_RIGHT_4_SEQUENCE=GGGGAAGCAGCCTTGTAAAC
PRIMER_INTERNAL_4_SEQUENCE=CCTCTAACACTTCCTTGCACTTG
PRIMER_LEFT_4=45,25
PRIMER_RIGHT_4=119,20
PRIMER_INTERNAL_4=75,23
PRIMER_LEFT_4_TM=57.173
PRIMER_RIGHT_4_TM=60.987
PRIMER_INTERNAL_4_TM=60.334
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH1227
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTGATAATTCACATACCATAAAATCCAACCATGGAAGGCACACAGTTCAGCAATTTTNAGTCTAGTCACAGAGTNGTGCGATCACCACTACAGTCTAATTTCAGATGTTTTCATTACCCCTAAAAGAAATCTTGTACCCATTAGCAATTATTCCTCATTCCTGCCCTCACCCCCAGGCCCTACTCTTTATCGCTATAGATTTGCCCACTTGACATATCATACACATGGAGCCATACATATGTGTGCCCTTCATGATTGGCTTCTTTCACTGAGAATAATGTTTTCAAGGT
SEQUENCE_INCLUDED_REGION=36,291
P3_COMMENT=CL is CCATAAAATCCAACCATGGA
P3_COMMENT=CR is TCATGAAGGGCACACATATG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.188598
PRIMER_LEFT_0_PENALTY=0.562302
PRIMER_RIGHT_0_PENALTY=0.626296
PRIMER_INTERNAL_0_PENALTY=1.249969
PRIMER_LEFT_0_SEQUENCE=CCCCAGGCCCTACTCTTTAT
PRIMER_RIGHT_0_SEQUENCE=CAATCATGAAGGGCACACAT
PRIMER_INTERNAL_0_SEQUENCE=CGCTATAGATTTGCCCACTTG
PRIMER_LEFT_0=207,20
PRIMER_RIGHT_0=294,20
PRIMER_INTERNAL_0=227,21
PRIMER_LEFT_0_TM=59.438
PRIMER_RIGHT_0_TM=59.374
PRIMER_INTERNAL_0_TM=59.750
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.2000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=1.436802
PRIMER_LEFT_1_PENALTY=0.562302
PRIMER_RIGHT_1_PENALTY=0.874500
PRIMER_INTERNAL_1_PENALTY=1.249969
PRIMER_LEFT_1_SEQUENCE=CCCCAGGCCCTACTCTTTAT
PRIMER_RIGHT_1_SEQUENCE=AAGAAGCCAATCATGAAGGG
PRIMER_INTERNAL_1_SEQUENCE=CGCTATAGATTTGCCCACTTG
PRIMER_LEFT_1=207,20
PRIMER_RIGHT_1=301,20
PRIMER_INTERNAL_1=227,21
PRIMER_LEFT_1_TM=59.438
PRIMER_RIGHT_1_TM=59.126
PRIMER_INTERNAL_1_TM=59.750
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=1.653378
PRIMER_LEFT_2_PENALTY=0.562302
PRIMER_RIGHT_2_PENALTY=1.091076
PRIMER_INTERNAL_2_PENALTY=1.249969
PRIMER_LEFT_2_SEQUENCE=CCCCAGGCCCTACTCTTTAT
PRIMER_RIGHT_2_SEQUENCE=CCAATCATGAAGGGCACAC
PRIMER_INTERNAL_2_SEQUENCE=CGCTATAGATTTGCCCACTTG
PRIMER_LEFT_2=207,20
PRIMER_RIGHT_2=295,19
PRIMER_INTERNAL_2=227,21
PRIMER_LEFT_2_TM=59.438
PRIMER_RIGHT_2_TM=59.909
PRIMER_INTERNAL_2_TM=59.750
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=52.632
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=1.674482
PRIMER_LEFT_3_PENALTY=1.048185
PRIMER_RIGHT_3_PENALTY=0.626296
PRIMER_INTERNAL_3_PENALTY=2.170825
PRIMER_LEFT_3_SEQUENCE=CAGGCCCTACTCTTTATCGC
PRIMER_RIGHT_3_SEQUENCE=CAATCATGAAGGGCACACAT
PRIMER_INTERNAL_3_SEQUENCE=GATTTGCCCACTTGACATATCA
PRIMER_LEFT_3=210,20
PRIMER_RIGHT_3=294,20
PRIMER_INTERNAL_3=234,22
PRIMER_LEFT_3_TM=58.952
PRIMER_RIGHT_3_TM=59.374
PRIMER_INTERNAL_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=9.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=1.741440
PRIMER_LEFT_4_PENALTY=1.115144
PRIMER_RIGHT_4_PENALTY=0.626296
PRIMER_INTERNAL_4_PENALTY=2.170825
PRIMER_LEFT_4_SEQUENCE=CAGGCCCTACTCTTTATCGCT
PRIMER_RIGHT_4_SEQUENCE=CAATCATGAAGGGCACACAT
PRIMER_INTERNAL_4_SEQUENCE=GATTTGCCCACTTGACATATCA
PRIMER_LEFT_4=210,21
PRIMER_RIGHT_4=294,20
PRIMER_INTERNAL_4=234,22
PRIMER_LEFT_4_TM=59.885
PRIMER_RIGHT_4_TM=59.374
PRIMER_INTERNAL_4_TM=59.829
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH1228
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTTGCATGAACAAAATTGGAAAGGAATGTATTCTGTTTNTCTGAATTTCCTTAATTTGCTTCTTTCCTTAATGTNCAAATTTGAGATGTGTGATATCAACTTGGGAGACATATATCAACGATAATATTTTATAAGAGCATTTTCTAAAATAATACAAAAATGCAGAATAATAGGATGGATTTGTTGGAGAAATAATGGGCTTTGAATCTGGATATTTCTGGGTTCAGATCTCAGCCCTGCCACTTACTCATTACATGACCTTGCATGTGTCACTTCCCCTCTCAAAGTC
SEQUENCE_INCLUDED_REGION=36,288
P3_COMMENT=CL is TGAGATGTGTGATATCAACTTGG
P3_COMMENT=CR is GAGAGGGGAAGTGACACATG
P3_COMMENT=CM is ACAAAAATGCAGAATAATAGGATGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.586514
PRIMER_LEFT_0_PENALTY=1.466705
PRIMER_RIGHT_0_PENALTY=0.119809
PRIMER_INTERNAL_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=TGGAGAAATAATGGGCTTTGA
PRIMER_RIGHT_0_SEQUENCE=AGAGGGGAAGTGACACATGC
PRIMER_INTERNAL_0_SEQUENCE=CTCAGCCCTGCCACTTACTC
PRIMER_LEFT_0=219,21
PRIMER_RIGHT_0=316,20
PRIMER_INTERNAL_0=264,20
PRIMER_LEFT_0_TM=59.533
PRIMER_RIGHT_0_TM=60.120
PRIMER_INTERNAL_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=1.666329
PRIMER_LEFT_1_PENALTY=1.546520
PRIMER_RIGHT_1_PENALTY=0.119809
PRIMER_INTERNAL_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=AATGGGCTTTGAATCTGGAT
PRIMER_RIGHT_1_SEQUENCE=AGAGGGGAAGTGACACATGC
PRIMER_INTERNAL_1_SEQUENCE=CTCAGCCCTGCCACTTACTC
PRIMER_LEFT_1=228,20
PRIMER_RIGHT_1=316,20
PRIMER_INTERNAL_1=264,20
PRIMER_LEFT_1_TM=58.453
PRIMER_RIGHT_1_TM=60.120
PRIMER_INTERNAL_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=89
PRIMER_PAIR_2_PENALTY=1.959681
PRIMER_LEFT_2_PENALTY=1.839872
PRIMER_RIGHT_2_PENALTY=0.119809
PRIMER_INTERNAL_2_PENALTY=0.012470
PRIMER_LEFT_2_SEQUENCE=TAATGGGCTTTGAATCTGGA
PRIMER_RIGHT_2_SEQUENCE=AGAGGGGAAGTGACACATGC
PRIMER_INTERNAL_2_SEQUENCE=CTCAGCCCTGCCACTTACTC
PRIMER_LEFT_2=227,20
PRIMER_RIGHT_2=316,20
PRIMER_INTERNAL_2=264,20
PRIMER_LEFT_2_TM=58.160
PRIMER_RIGHT_2_TM=60.120
PRIMER_INTERNAL_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=2.055020
PRIMER_LEFT_3_PENALTY=1.935211
PRIMER_RIGHT_3_PENALTY=0.119809
PRIMER_INTERNAL_3_PENALTY=0.012470
PRIMER_LEFT_3_SEQUENCE=TTGGAGAAATAATGGGCTTTG
PRIMER_RIGHT_3_SEQUENCE=AGAGGGGAAGTGACACATGC
PRIMER_INTERNAL_3_SEQUENCE=CTCAGCCCTGCCACTTACTC
PRIMER_LEFT_3=218,21
PRIMER_RIGHT_3=316,20
PRIMER_INTERNAL_3=264,20
PRIMER_LEFT_3_TM=59.065
PRIMER_RIGHT_3_TM=60.120
PRIMER_INTERNAL_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=2.276288
PRIMER_LEFT_4_PENALTY=2.156479
PRIMER_RIGHT_4_PENALTY=0.119809
PRIMER_INTERNAL_4_PENALTY=0.012470
PRIMER_LEFT_4_SEQUENCE=GTTGGAGAAATAATGGGCTTTG
PRIMER_RIGHT_4_SEQUENCE=AGAGGGGAAGTGACACATGC
PRIMER_INTERNAL_4_SEQUENCE=CTCAGCCCTGCCACTTACTC
PRIMER_LEFT_4=217,22
PRIMER_RIGHT_4=316,20
PRIMER_INTERNAL_4=264,20
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=60.120
PRIMER_INTERNAL_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH1230
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCCTCCACCTCTGGCCCACACACCAAATCACACTCTCTACATTTTCTGATTTCTGTATTGAGACCAAGTTTTGATTTATATTTCTGAGGGGTCAATCTGCAATTGACCNACGTGCCAAATCTGTAGACTATATCCAGTTTTCAAACTTATTTCCACATCCTTCAAAACAACTGCTCACACCATTGCTCATCTCCCTACTTCTGCATTTCAACACTACTCAGGGAGACGTGGGACAGNANGNTCTGTGGCACAGCACAGGCCCACAGTGGGCTCATCTAGTGCACTGTGTCTGGACCCTGCTCTGC
SEQUENCE_INCLUDED_REGION=36,307
P3_COMMENT=CL is CCACACACCAAATCACACTC
P3_COMMENT=CR is ACACAGTGCACTAGATGAGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.426859
PRIMER_LEFT_0_PENALTY=0.111814
PRIMER_RIGHT_0_PENALTY=0.315045
PRIMER_INTERNAL_0_PENALTY=0.668065
PRIMER_LEFT_0_SEQUENCE=TACTCAGGGAGACGTGGGAC
PRIMER_RIGHT_0_SEQUENCE=GAGCAGGGTCCAGACACAGT
PRIMER_INTERNAL_0_SEQUENCE=CCCACAGTGGGCTCATCTAG
PRIMER_LEFT_0=252,20
PRIMER_RIGHT_0=339,20
PRIMER_INTERNAL_0=297,20
PRIMER_LEFT_0_TM=60.112
PRIMER_RIGHT_0_TM=60.315
PRIMER_INTERNAL_0_TM=60.668
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=9.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.567294
PRIMER_LEFT_1_PENALTY=0.252249
PRIMER_RIGHT_1_PENALTY=0.315045
PRIMER_INTERNAL_1_PENALTY=0.668065
PRIMER_LEFT_1_SEQUENCE=CTACTCAGGGAGACGTGGGA
PRIMER_RIGHT_1_SEQUENCE=GAGCAGGGTCCAGACACAGT
PRIMER_INTERNAL_1_SEQUENCE=CCCACAGTGGGCTCATCTAG
PRIMER_LEFT_1=251,20
PRIMER_RIGHT_1=339,20
PRIMER_INTERNAL_1=297,20
PRIMER_LEFT_1_TM=60.252
PRIMER_RIGHT_1_TM=60.315
PRIMER_INTERNAL_1_TM=60.668
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=9.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=89
PRIMER_PAIR_2_PENALTY=0.569545
PRIMER_LEFT_2_PENALTY=0.111814
PRIMER_RIGHT_2_PENALTY=0.457731
PRIMER_INTERNAL_2_PENALTY=0.008216
PRIMER_LEFT_2_SEQUENCE=TACTCAGGGAGACGTGGGAC
PRIMER_RIGHT_2_SEQUENCE=AGAGCAGGGTCCAGACACAG
PRIMER_INTERNAL_2_SEQUENCE=CAGTGGGCTCATCTAGTGCA
PRIMER_LEFT_2=252,20
PRIMER_RIGHT_2=340,20
PRIMER_INTERNAL_2=301,20
PRIMER_LEFT_2_TM=60.112
PRIMER_RIGHT_2_TM=60.458
PRIMER_INTERNAL_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=0.709980
PRIMER_LEFT_3_PENALTY=0.252249
PRIMER_RIGHT_3_PENALTY=0.457731
PRIMER_INTERNAL_3_PENALTY=0.008216
PRIMER_LEFT_3_SEQUENCE=CTACTCAGGGAGACGTGGGA
PRIMER_RIGHT_3_SEQUENCE=AGAGCAGGGTCCAGACACAG
PRIMER_INTERNAL_3_SEQUENCE=CAGTGGGCTCATCTAGTGCA
PRIMER_LEFT_3=251,20
PRIMER_RIGHT_3=340,20
PRIMER_INTERNAL_3=301,20
PRIMER_LEFT_3_TM=60.252
PRIMER_RIGHT_3_TM=60.458
PRIMER_INTERNAL_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=0.789588
PRIMER_LEFT_4_PENALTY=0.716637
PRIMER_RIGHT_4_PENALTY=0.072951
PRIMER_INTERNAL_4_PENALTY=5.746125
PRIMER_LEFT_4_SEQUENCE=CCCACACACCAAATCACACT
PRIMER_RIGHT_4_SEQUENCE=ATTGCAGATTGACCCCTCAG
PRIMER_INTERNAL_4_SEQUENCE=TTTCTGTATTGAGACCAAGTTTTGA
PRIMER_LEFT_4=52,20
PRIMER_RIGHT_4=140,20
PRIMER_INTERNAL_4=87,25
PRIMER_LEFT_4_TM=59.283
PRIMER_RIGHT_4_TM=60.073
PRIMER_INTERNAL_4_TM=59.254
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH1231
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTCCCCTCCTACGTNAGAGTNNATACCCAAGGAAGCGGAAGTAGAAGATGACTCTTCAAGAGANNTATCCCCTGCTGTTACATGTATACATACANGATACATACATATGGAATGCATGGGAAAGGAAGGCATTAGAATTCCACCTGAACTTTCTGTGCTTAAAAGATTAGAGGAGACAGTGTACAAAAAGAGAGATCATTGATTCTCCGCCCACTGCCTTGACTCTATGTGTGTTTTCTATTTTGATACTGTTGCTCCTCTTATTCACATGCTGTTTAAAATATACAGGGGTACCGAGNCTCGAATTCGTAATCATGGTCATAGCTGT
SEQUENCE_INCLUDED_REGION=36,291
P3_COMMENT=CL is CAAGGAAGCGGAAGTAGAAG
P3_COMMENT=CR is CATAGAGTCAAGGCAGTGGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.111146
PRIMER_LEFT_0_PENALTY=0.298125
PRIMER_RIGHT_0_PENALTY=0.813021
PRIMER_INTERNAL_0_PENALTY=5.287628
PRIMER_LEFT_0_SEQUENCE=TACCCAAGGAAGCGGAAGTA
PRIMER_RIGHT_0_SEQUENCE=TTTCCCATGCATTCCATATG
PRIMER_INTERNAL_0_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
PRIMER_LEFT_0=62,20
PRIMER_RIGHT_0=161,20
PRIMER_INTERNAL_0=106,25
PRIMER_LEFT_0_TM=59.702
PRIMER_RIGHT_0_TM=59.187
PRIMER_INTERNAL_0_TM=59.712
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=5.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=1.212472
PRIMER_LEFT_1_PENALTY=0.552282
PRIMER_RIGHT_1_PENALTY=0.660189
PRIMER_INTERNAL_1_PENALTY=5.287628
PRIMER_LEFT_1_SEQUENCE=CCAAGGAAGCGGAAGTAGAA
PRIMER_RIGHT_1_SEQUENCE=CCTTTCCCATGCATTCCATA
PRIMER_INTERNAL_1_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
PRIMER_LEFT_1=65,20
PRIMER_RIGHT_1=163,20
PRIMER_INTERNAL_1=106,25
PRIMER_LEFT_1_TM=59.448
PRIMER_RIGHT_1_TM=60.660
PRIMER_INTERNAL_1_TM=59.712
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=1.328827
PRIMER_LEFT_2_PENALTY=0.298125
PRIMER_RIGHT_2_PENALTY=1.030702
PRIMER_INTERNAL_2_PENALTY=5.287628
PRIMER_LEFT_2_SEQUENCE=TACCCAAGGAAGCGGAAGTA
PRIMER_RIGHT_2_SEQUENCE=TTTCCCATGCATTCCATATGT
PRIMER_INTERNAL_2_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
PRIMER_LEFT_2=62,20
PRIMER_RIGHT_2=161,21
PRIMER_INTERNAL_2=106,25
PRIMER_LEFT_2_TM=59.702
PRIMER_RIGHT_2_TM=60.031
PRIMER_INTERNAL_2_TM=59.712
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=10.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=8.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=1.365304
PRIMER_LEFT_3_PENALTY=0.552282
PRIMER_RIGHT_3_PENALTY=0.813021
PRIMER_INTERNAL_3_PENALTY=5.287628
PRIMER_LEFT_3_SEQUENCE=CCAAGGAAGCGGAAGTAGAA
PRIMER_RIGHT_3_SEQUENCE=TTTCCCATGCATTCCATATG
PRIMER_INTERNAL_3_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
PRIMER_LEFT_3=65,20
PRIMER_RIGHT_3=161,20
PRIMER_INTERNAL_3=106,25
PRIMER_LEFT_3_TM=59.448
PRIMER_RIGHT_3_TM=59.187
PRIMER_INTERNAL_3_TM=59.712
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=1.392215
PRIMER_LEFT_4_PENALTY=0.038361
PRIMER_RIGHT_4_PENALTY=1.353854
PRIMER_INTERNAL_4_PENALTY=5.287628
PRIMER_LEFT_4_SEQUENCE=ATACCCAAGGAAGCGGAAGT
PRIMER_RIGHT_4_SEQUENCE=TTCCCATGCATTCCATATGT
PRIMER_INTERNAL_4_SEQUENCE=TCCCCTGCTGTTACATGTATACATA
PRIMER_LEFT_4=61,20
PRIMER_RIGHT_4=160,20
PRIMER_INTERNAL_4=106,25
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=58.646
PRIMER_INTERNAL_4_TM=59.712
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH1232
SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTACCCACCCCCTTCACCCCACCTGAATGAGATGATTTCTGAAGTCCTCAGCAGCCCTGATTCTAAGCCTCATAAGGAAGAGTAGGTGTTAATGGCATCCTAGGGCAATGGTAGGTGCCTGATGCAGATCTGCTGTGAGCCATGTGCTGGCATCACAGGGGTGGTTTATTAATTTCATTTATCATCTGGACAGCCCCTTCTTATAACGTACATCCTTGCCTCTTCTGAGGCGCTAAGATCCCCAAGGTGGCTCCTGTATCCAGAAACCCNTNGGCCTTCCCTCCCAGTTA
SEQUENCE_INCLUDED_REGION=34,289
P3_COMMENT=CL is GATGATTTCTGAAGTCCTCAGC
P3_COMMENT=CR is TTTCTGGATACAGGAGCCAC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.157984
PRIMER_LEFT_0_PENALTY=0.051674
PRIMER_RIGHT_0_PENALTY=0.106310
PRIMER_INTERNAL_0_PENALTY=0.305090
PRIMER_LEFT_0_SEQUENCE=TCCTAGGGCAATGGTAGGTG
PRIMER_RIGHT_0_SEQUENCE=GGGGCTGTCCAGATGATAAA
PRIMER_INTERNAL_0_SEQUENCE=GATGCAGATCTGCTGTGAGC
PRIMER_LEFT_0=130,20
PRIMER_RIGHT_0=229,20
PRIMER_INTERNAL_0=153,20
PRIMER_LEFT_0_TM=59.948
PRIMER_RIGHT_0_TM=59.894
PRIMER_INTERNAL_0_TM=59.695
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.179262
PRIMER_LEFT_1_PENALTY=0.106310
PRIMER_RIGHT_1_PENALTY=0.072951
PRIMER_INTERNAL_1_PENALTY=0.056936
PRIMER_LEFT_1_SEQUENCE=TTTATCATCTGGACAGCCCC
PRIMER_RIGHT_1_SEQUENCE=TTCTGGATACAGGAGCCACC
PRIMER_INTERNAL_1_SEQUENCE=CATCCTTGCCTCTTCTGAGG
PRIMER_LEFT_1=210,20
PRIMER_RIGHT_1=297,20
PRIMER_INTERNAL_1=243,20
PRIMER_LEFT_1_TM=59.894
PRIMER_RIGHT_1_TM=60.073
PRIMER_INTERNAL_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=0.209924
PRIMER_LEFT_2_PENALTY=0.103613
PRIMER_RIGHT_2_PENALTY=0.106310
PRIMER_INTERNAL_2_PENALTY=0.323721
PRIMER_LEFT_2_SEQUENCE=GGTAGGTGCCTGATGCAGAT
PRIMER_RIGHT_2_SEQUENCE=GGGGCTGTCCAGATGATAAA
PRIMER_INTERNAL_2_SEQUENCE=GCATCACAGGGGTGGTTTAT
PRIMER_LEFT_2=142,20
PRIMER_RIGHT_2=229,20
PRIMER_INTERNAL_2=182,20
PRIMER_LEFT_2_TM=60.104
PRIMER_RIGHT_2_TM=59.894
PRIMER_INTERNAL_2_TM=59.676
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=0.221430
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.103613
PRIMER_INTERNAL_3_PENALTY=0.077340
PRIMER_LEFT_3_SEQUENCE=CAGCAGCCCTGATTCTAAGC
PRIMER_RIGHT_3_SEQUENCE=ATCTGCATCAGGCACCTACC
PRIMER_INTERNAL_3_SEQUENCE=GTTAATGGCATCCTAGGGCA
PRIMER_LEFT_3=81,20
PRIMER_RIGHT_3=161,20
PRIMER_INTERNAL_3=120,20
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.104
PRIMER_INTERNAL_3_TM=59.923
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=0.340133
PRIMER_LEFT_4_PENALTY=0.262792
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_INTERNAL_4_PENALTY=0.117817
PRIMER_LEFT_4_SEQUENCE=CCCCACCTGAATGAGATGAT
PRIMER_RIGHT_4_SEQUENCE=GCCCTAGGATGCCATTAACA
PRIMER_INTERNAL_4_SEQUENCE=CAGCAGCCCTGATTCTAAGC
PRIMER_LEFT_4=49,20
PRIMER_RIGHT_4=138,20
PRIMER_INTERNAL_4=81,20
PRIMER_LEFT_4_TM=59.737
PRIMER_RIGHT_4_TM=59.923
PRIMER_INTERNAL_4_TM=60.118
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH1233
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATATTATAGAACATTATATTTGCATGTATCCAAGTNGCATTATTGAAAAACTCATTTGGATNAACATTGAGATTTTAGGAGTNNCTTTAAAAGAGTNNTGTACATAACTACATTTAAGAAACTCCCTCATGACAATTCCATGAGATAGTTACTATTATCCACATTTTGCAGATGAAGATACTGAGACTTAACAAAAATAAGTAAAATTGTCTACGGTTATGAGTNTAGCAAGATACCAGTGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
SEQUENCE_INCLUDED_REGION=36,247
P3_COMMENT=CL is GAACATTATATTTGCATGTATCCA
P3_COMMENT=CR is CCGTAGACAATTTTACTTATTTTTG
P3_COMMENT=CM is GCATTATTGAAAAACTCATTTGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=10.747149
PRIMER_LEFT_0_PENALTY=4.460533
PRIMER_RIGHT_0_PENALTY=6.286617
PRIMER_INTERNAL_0_PENALTY=9.564502
PRIMER_LEFT_0_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
PRIMER_RIGHT_0_SEQUENCE=GTCTCAGTATCTTCATCTGCAAAA
PRIMER_INTERNAL_0_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
PRIMER_LEFT_0=150,24
PRIMER_RIGHT_0=226,24
PRIMER_INTERNAL_0=176,27
PRIMER_LEFT_0_TM=59.539
PRIMER_RIGHT_0_TM=57.713
PRIMER_INTERNAL_0_TM=57.435
PRIMER_LEFT_0_GC_PERCENT=37.500
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_INTERNAL_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=9.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=10.794372
PRIMER_LEFT_1_PENALTY=4.460533
PRIMER_RIGHT_1_PENALTY=6.333840
PRIMER_INTERNAL_1_PENALTY=9.564502
PRIMER_LEFT_1_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
PRIMER_RIGHT_1_SEQUENCE=AGTCTCAGTATCTTCATCTGCAAAA
PRIMER_INTERNAL_1_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
PRIMER_LEFT_1=150,24
PRIMER_RIGHT_1=227,25
PRIMER_INTERNAL_1=176,27
PRIMER_LEFT_1_TM=59.539
PRIMER_RIGHT_1_TM=58.666
PRIMER_INTERNAL_1_TM=57.435
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=36.000
PRIMER_INTERNAL_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=9.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=10.794372
PRIMER_LEFT_2_PENALTY=4.460533
PRIMER_RIGHT_2_PENALTY=6.333840
PRIMER_INTERNAL_2_PENALTY=9.564502
PRIMER_LEFT_2_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
PRIMER_RIGHT_2_SEQUENCE=AAGTCTCAGTATCTTCATCTGCAAA
PRIMER_INTERNAL_2_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
PRIMER_LEFT_2=150,24
PRIMER_RIGHT_2=228,25
PRIMER_INTERNAL_2=176,27
PRIMER_LEFT_2_TM=59.539
PRIMER_RIGHT_2_TM=58.666
PRIMER_INTERNAL_2_TM=57.435
PRIMER_LEFT_2_GC_PERCENT=37.500
PRIMER_RIGHT_2_GC_PERCENT=36.000
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=9.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=11.114895
PRIMER_LEFT_3_PENALTY=4.460533
PRIMER_RIGHT_3_PENALTY=6.654362
PRIMER_INTERNAL_3_PENALTY=9.564502
PRIMER_LEFT_3_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
PRIMER_RIGHT_3_SEQUENCE=AGTCTCAGTATCTTCATCTGCAAA
PRIMER_INTERNAL_3_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
PRIMER_LEFT_3=150,24
PRIMER_RIGHT_3=227,24
PRIMER_INTERNAL_3=176,27
PRIMER_LEFT_3_TM=59.539
PRIMER_RIGHT_3_TM=57.346
PRIMER_INTERNAL_3_TM=57.435
PRIMER_LEFT_3_GC_PERCENT=37.500
PRIMER_RIGHT_3_GC_PERCENT=37.500
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=9.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=11.114895
PRIMER_LEFT_4_PENALTY=4.460533
PRIMER_RIGHT_4_PENALTY=6.654362
PRIMER_INTERNAL_4_PENALTY=9.564502
PRIMER_LEFT_4_SEQUENCE=CATTTAAGAAACTCCCTCATGACA
PRIMER_RIGHT_4_SEQUENCE=AAGTCTCAGTATCTTCATCTGCAA
PRIMER_INTERNAL_4_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA
PRIMER_LEFT_4=150,24
PRIMER_RIGHT_4=228,24
PRIMER_INTERNAL_4=176,27
PRIMER_LEFT_4_TM=59.539
PRIMER_RIGHT_4_TM=57.346
PRIMER_INTERNAL_4_TM=57.435
PRIMER_LEFT_4_GC_PERCENT=37.500
PRIMER_RIGHT_4_GC_PERCENT=37.500
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=9.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH1234
SEQUENCE_TEMPLATE=CTTGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTNGTNNCTCTTCTTTGTNGATCAGAAATTACAGCAGGAACTACTGCTACTGGGTNAGGAAACCTAAATGCAATGNGGATCACTGGATNCAGGGTNNCACTTTAGTCGCCAAAGACAAGGTGGNATGGNTACTATAATAGACATCAGAATCAAAGCAATAATCAGAATAGCCTAAGAGATGTATGGCATTGGCTAGTTGATCATAATGTCTCCACAAGTGAAGTAGNTNGGCAGTCAACAAAATTCTTACTTGATTTGTNTANGGGNAACAACTCCATATCAAGTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTG
SEQUENCE_INCLUDED_REGION=35,291
P3_COMMENT=CL is GAAATTACAGCAGGAACTACTGC
P3_COMMENT=CR is AAGTAAGAATTTTGTTGACTGCC
P3_COMMENT=CM is CACTTTAGTCGCCAAAGACAA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.353299
PRIMER_LEFT_0_PENALTY=2.133445
PRIMER_RIGHT_0_PENALTY=0.219854
PRIMER_INTERNAL_0_PENALTY=5.342972
PRIMER_LEFT_0_SEQUENCE=AGTCGCCAAAGACAAGGTG
PRIMER_RIGHT_0_SEQUENCE=TGATCAACTAGCCAATGCCA
PRIMER_INTERNAL_0_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
PRIMER_LEFT_0=142,19
PRIMER_RIGHT_0=241,20
PRIMER_INTERNAL_0=180,25
PRIMER_LEFT_0_TM=58.867
PRIMER_RIGHT_0_TM=60.220
PRIMER_INTERNAL_0_TM=59.657
PRIMER_LEFT_0_GC_PERCENT=52.632
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=32.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=2.765222
PRIMER_LEFT_1_PENALTY=2.545368
PRIMER_RIGHT_1_PENALTY=0.219854
PRIMER_INTERNAL_1_PENALTY=5.342972
PRIMER_LEFT_1_SEQUENCE=AGTCGCCAAAGACAAGGTGG
PRIMER_RIGHT_1_SEQUENCE=TGATCAACTAGCCAATGCCA
PRIMER_INTERNAL_1_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
PRIMER_LEFT_1=142,20
PRIMER_RIGHT_1=241,20
PRIMER_INTERNAL_1=180,25
PRIMER_LEFT_1_TM=62.545
PRIMER_RIGHT_1_TM=60.220
PRIMER_INTERNAL_1_TM=59.657
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=32.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=2.872243
PRIMER_LEFT_2_PENALTY=2.652389
PRIMER_RIGHT_2_PENALTY=0.219854
PRIMER_INTERNAL_2_PENALTY=5.342972
PRIMER_LEFT_2_SEQUENCE=GTCGCCAAAGACAAGGTGG
PRIMER_RIGHT_2_SEQUENCE=TGATCAACTAGCCAATGCCA
PRIMER_INTERNAL_2_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
PRIMER_LEFT_2=143,19
PRIMER_RIGHT_2=241,20
PRIMER_INTERNAL_2=180,25
PRIMER_LEFT_2_TM=61.652
PRIMER_RIGHT_2_TM=60.220
PRIMER_INTERNAL_2_TM=59.657
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=32.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=2.881005
PRIMER_LEFT_3_PENALTY=1.076949
PRIMER_RIGHT_3_PENALTY=1.804056
PRIMER_INTERNAL_3_PENALTY=5.342972
PRIMER_LEFT_3_SEQUENCE=TAGTCGCCAAAGACAAGGTG
PRIMER_RIGHT_3_SEQUENCE=TCAACTAGCCAATGCCATACA
PRIMER_INTERNAL_3_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
PRIMER_LEFT_3=141,20
PRIMER_RIGHT_3=238,21
PRIMER_INTERNAL_3=180,25
PRIMER_LEFT_3_TM=58.923
PRIMER_RIGHT_3_TM=59.196
PRIMER_INTERNAL_3_TM=59.657
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=32.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=3.026281
PRIMER_LEFT_4_PENALTY=2.806427
PRIMER_RIGHT_4_PENALTY=0.219854
PRIMER_INTERNAL_4_PENALTY=5.342972
PRIMER_LEFT_4_SEQUENCE=TCGCCAAAGACAAGGTGG
PRIMER_RIGHT_4_SEQUENCE=TGATCAACTAGCCAATGCCA
PRIMER_INTERNAL_4_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA
PRIMER_LEFT_4=144,18
PRIMER_RIGHT_4=241,20
PRIMER_INTERNAL_4=180,25
PRIMER_LEFT_4_TM=60.806
PRIMER_RIGHT_4_TM=60.220
PRIMER_INTERNAL_4_TM=59.657
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH1236
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTACATGTNNNACAGGTCTTGTTTCAAAAACTTAATCCCCATGAAAGTTTCTGNTTGTACCCCAGATGGTAGTGTGTAACTTGCTTCTCCATCAGGGAAAGTCCTGCTGTCCATGTGAGGCTCTGGCTCTNCCATTTTCCACCCCTGGGCTGTGATCACAGCAGAGCAGGCTTCTAGAATNCATGTCCCCAGCACCCCATCCTTGCAGCAATGGCGAGGAGACAGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC
SEQUENCE_INCLUDED_REGION=36,230
P3_COMMENT=CL is TTGTTTCAAAAACTTAATCCCC
P3_COMMENT=CR is GTCTCCTCGCCATTGCTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.706826
PRIMER_LEFT_0_PENALTY=0.196903
PRIMER_RIGHT_0_PENALTY=0.509923
PRIMER_INTERNAL_0_PENALTY=0.738752
PRIMER_LEFT_0_SEQUENCE=ACTTGCTTCTCCATCAGGGA
PRIMER_RIGHT_0_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
PRIMER_INTERNAL_0_SEQUENCE=AAGTCCTGCTGTCCATGTGA
PRIMER_LEFT_0=114,20
PRIMER_RIGHT_0=211,20
PRIMER_INTERNAL_0=134,20
PRIMER_LEFT_0_TM=59.803
PRIMER_RIGHT_0_TM=59.490
PRIMER_INTERNAL_0_TM=59.261
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=0.840967
PRIMER_LEFT_1_PENALTY=0.331043
PRIMER_RIGHT_1_PENALTY=0.509923
PRIMER_INTERNAL_1_PENALTY=1.003769
PRIMER_LEFT_1_SEQUENCE=TGCTTCTCCATCAGGGAAAG
PRIMER_RIGHT_1_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
PRIMER_INTERNAL_1_SEQUENCE=CTGCTGTCCATGTGAGGCT
PRIMER_LEFT_1=117,20
PRIMER_RIGHT_1=211,20
PRIMER_INTERNAL_1=139,19
PRIMER_LEFT_1_TM=60.331
PRIMER_RIGHT_1_TM=59.490
PRIMER_INTERNAL_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=0.840967
PRIMER_LEFT_2_PENALTY=0.331043
PRIMER_RIGHT_2_PENALTY=0.509923
PRIMER_INTERNAL_2_PENALTY=1.003769
PRIMER_LEFT_2_SEQUENCE=CTTGCTTCTCCATCAGGGAA
PRIMER_RIGHT_2_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
PRIMER_INTERNAL_2_SEQUENCE=CTGCTGTCCATGTGAGGCT
PRIMER_LEFT_2=115,20
PRIMER_RIGHT_2=211,20
PRIMER_INTERNAL_2=139,19
PRIMER_LEFT_2_TM=60.331
PRIMER_RIGHT_2_TM=59.490
PRIMER_INTERNAL_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=0.861071
PRIMER_LEFT_3_PENALTY=0.351148
PRIMER_RIGHT_3_PENALTY=0.509923
PRIMER_INTERNAL_3_PENALTY=1.853206
PRIMER_LEFT_3_SEQUENCE=CTCCATCAGGGAAAGTCCTG
PRIMER_RIGHT_3_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
PRIMER_INTERNAL_3_SEQUENCE=TGTCCATGTGAGGCTCTGG
PRIMER_LEFT_3=122,20
PRIMER_RIGHT_3=211,20
PRIMER_INTERNAL_3=143,19
PRIMER_LEFT_3_TM=59.649
PRIMER_RIGHT_3_TM=59.490
PRIMER_INTERNAL_3_TM=60.853
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=0.895679
PRIMER_LEFT_4_PENALTY=0.385756
PRIMER_RIGHT_4_PENALTY=0.509923
PRIMER_INTERNAL_4_PENALTY=1.910613
PRIMER_LEFT_4_SEQUENCE=TCAGGGAAAGTCCTGCTGTC
PRIMER_RIGHT_4_SEQUENCE=TAGAAGCCTGCTCTGCTGTG
PRIMER_INTERNAL_4_SEQUENCE=CATGTGAGGCTCTGGCTCT
PRIMER_LEFT_4=127,20
PRIMER_RIGHT_4=211,20
PRIMER_INTERNAL_4=147,19
PRIMER_LEFT_4_TM=60.386
PRIMER_RIGHT_4_TM=59.490
PRIMER_INTERNAL_4_TM=59.089
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH1237
SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCTTTTCTTTCAGTNCTTCATTTCCANNATCGACTTGTGAGAAAAATGTCTTTGACACAGAAAGAAGTGCTTCTCTGGGCCACTGTNNNTCAAGGAAAAATCTAGAAAATCTGTTGTNTNATTTCAATAACATACAGTTTAATACCAAGAAAAACATATTTCTCGGGAACTCCTCCCTGCCCAGTNCCCTCTTCACCTAGTTAACTCCTACTCATGTCTATGGTGCTTAATGAACTGAAGGGCTCTGTNCATTTGAGGGNTGCCTGGGTNTGCATTTAGTGACCAGTATGTCTCCACAGTATCATGCACACGGA
SEQUENCE_INCLUDED_REGION=36,312
P3_COMMENT=CL is GTGAGAAAAATGTCTTTGACACA
P3_COMMENT=CR is GCCCTTCAGTTCATTAAGCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.900545
PRIMER_LEFT_0_PENALTY=2.013682
PRIMER_RIGHT_0_PENALTY=0.886863
PRIMER_INTERNAL_0_PENALTY=5.661030
PRIMER_LEFT_0_SEQUENCE=TGCTTAATGAACTGAAGGGCT
PRIMER_RIGHT_0_SEQUENCE=TCCGTGTGCATGATACTGTG
PRIMER_INTERNAL_0_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
PRIMER_LEFT_0=257,21
PRIMER_RIGHT_0=347,20
PRIMER_INTERNAL_0=304,24
PRIMER_LEFT_0_TM=58.986
PRIMER_RIGHT_0_TM=59.113
PRIMER_INTERNAL_0_TM=58.339
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=2.915660
PRIMER_LEFT_1_PENALTY=2.028797
PRIMER_RIGHT_1_PENALTY=0.886863
PRIMER_INTERNAL_1_PENALTY=5.661030
PRIMER_LEFT_1_SEQUENCE=TGCTTAATGAACTGAAGGGC
PRIMER_RIGHT_1_SEQUENCE=TCCGTGTGCATGATACTGTG
PRIMER_INTERNAL_1_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
PRIMER_LEFT_1=257,20
PRIMER_RIGHT_1=347,20
PRIMER_INTERNAL_1=304,24
PRIMER_LEFT_1_TM=57.971
PRIMER_RIGHT_1_TM=59.113
PRIMER_INTERNAL_1_TM=58.339
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=3.037245
PRIMER_LEFT_2_PENALTY=2.150382
PRIMER_RIGHT_2_PENALTY=0.886863
PRIMER_INTERNAL_2_PENALTY=5.661030
PRIMER_LEFT_2_SEQUENCE=GTGCTTAATGAACTGAAGGGC
PRIMER_RIGHT_2_SEQUENCE=TCCGTGTGCATGATACTGTG
PRIMER_INTERNAL_2_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
PRIMER_LEFT_2=256,21
PRIMER_RIGHT_2=347,20
PRIMER_INTERNAL_2=304,24
PRIMER_LEFT_2_TM=58.850
PRIMER_RIGHT_2_TM=59.113
PRIMER_INTERNAL_2_TM=58.339
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=3.097749
PRIMER_LEFT_3_PENALTY=2.210886
PRIMER_RIGHT_3_PENALTY=0.886863
PRIMER_INTERNAL_3_PENALTY=5.661030
PRIMER_LEFT_3_SEQUENCE=GTGCTTAATGAACTGAAGGGCT
PRIMER_RIGHT_3_SEQUENCE=TCCGTGTGCATGATACTGTG
PRIMER_INTERNAL_3_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
PRIMER_LEFT_3=256,22
PRIMER_RIGHT_3=347,20
PRIMER_INTERNAL_3=304,24
PRIMER_LEFT_3_TM=59.789
PRIMER_RIGHT_3_TM=59.113
PRIMER_INTERNAL_3_TM=58.339
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=3.179952
PRIMER_LEFT_4_PENALTY=2.293089
PRIMER_RIGHT_4_PENALTY=0.886863
PRIMER_INTERNAL_4_PENALTY=5.661030
PRIMER_LEFT_4_SEQUENCE=GGTGCTTAATGAACTGAAGGG
PRIMER_RIGHT_4_SEQUENCE=TCCGTGTGCATGATACTGTG
PRIMER_INTERNAL_4_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC
PRIMER_LEFT_4=255,21
PRIMER_RIGHT_4=347,20
PRIMER_INTERNAL_4=304,24
PRIMER_LEFT_4_TM=58.707
PRIMER_RIGHT_4_TM=59.113
PRIMER_INTERNAL_4_TM=58.339
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH1238
SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTGTNGTNTTGACTGTTTGTTTGTTTTTTTACTGCCTACAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTT
SEQUENCE_INCLUDED_REGION=35,44
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1239
SEQUENCE_TEMPLATE=TTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACAACTGTGAGCAGCAATGCCGTCTTTCAGTATAGCATGCTTCATATTTGCAGAGATCCGCATGCACATAATATTCTCACCATCACAACACAGTTAAGTATGCTTGCACACGTGTGTACACACACACACACACACACACACACACTGAGAAGGCAGTCAGTGACTACCTTCCATTTTCCAACCACAAACTGCTGTCAGAATGAATACTACGTTTTAGCTTTTCAAATAACTGGAAATAACATCAGATTAAGTAAAGAAGACAGGGTAC
SEQUENCE_INCLUDED_REGION=34,271
P3_COMMENT=CL is GCAATGCCGTCTTTCAGTAT
P3_COMMENT=CR is AAACGTAGTATTCATTCTGACAGC
P3_COMMENT=CM is GCATGCTTCATATTTGCAGA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.228181
PRIMER_LEFT_0_PENALTY=0.207669
PRIMER_RIGHT_0_PENALTY=1.020512
PRIMER_INTERNAL_0_PENALTY=3.883941
PRIMER_LEFT_0_SEQUENCE=TATTTGCAGAGATCCGCATG
PRIMER_RIGHT_0_SEQUENCE=TGTACACACGTGTGCAAGCA
PRIMER_INTERNAL_0_SEQUENCE=TTCTCACCATCACAACACAGTT
PRIMER_LEFT_0=81,20
PRIMER_RIGHT_0=156,20
PRIMER_INTERNAL_0=110,22
PRIMER_LEFT_0_TM=59.792
PRIMER_RIGHT_0_TM=61.021
PRIMER_INTERNAL_0_TM=58.116
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=10.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=7.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=1.228181
PRIMER_LEFT_1_PENALTY=0.207669
PRIMER_RIGHT_1_PENALTY=1.020512
PRIMER_INTERNAL_1_PENALTY=3.883941
PRIMER_LEFT_1_SEQUENCE=CATATTTGCAGAGATCCGCA
PRIMER_RIGHT_1_SEQUENCE=TGTACACACGTGTGCAAGCA
PRIMER_INTERNAL_1_SEQUENCE=TTCTCACCATCACAACACAGTT
PRIMER_LEFT_1=79,20
PRIMER_RIGHT_1=156,20
PRIMER_INTERNAL_1=110,22
PRIMER_LEFT_1_TM=59.792
PRIMER_RIGHT_1_TM=61.021
PRIMER_INTERNAL_1_TM=58.116
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=1.286948
PRIMER_LEFT_2_PENALTY=1.020512
PRIMER_RIGHT_2_PENALTY=0.266437
PRIMER_INTERNAL_2_PENALTY=1.079874
PRIMER_LEFT_2_SEQUENCE=TGCTTGCACACGTGTGTACA
PRIMER_RIGHT_2_SEQUENCE=CAGCAGTTTGTGGTTGGAAA
PRIMER_INTERNAL_2_SEQUENCE=CACACACTGAGAAGGCAGTCA
PRIMER_LEFT_2=137,20
PRIMER_RIGHT_2=230,20
PRIMER_INTERNAL_2=175,21
PRIMER_LEFT_2_TM=61.021
PRIMER_RIGHT_2_TM=59.734
PRIMER_INTERNAL_2_TM=60.080
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=8.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=1.294259
PRIMER_LEFT_3_PENALTY=1.020512
PRIMER_RIGHT_3_PENALTY=0.273747
PRIMER_INTERNAL_3_PENALTY=1.079874
PRIMER_LEFT_3_SEQUENCE=TGCTTGCACACGTGTGTACA
PRIMER_RIGHT_3_SEQUENCE=GACAGCAGTTTGTGGTTGGA
PRIMER_INTERNAL_3_SEQUENCE=CACACACTGAGAAGGCAGTCA
PRIMER_LEFT_3=137,20
PRIMER_RIGHT_3=232,20
PRIMER_INTERNAL_3=175,21
PRIMER_LEFT_3_TM=61.021
PRIMER_RIGHT_3_TM=59.726
PRIMER_INTERNAL_3_TM=60.080
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=1.389613
PRIMER_LEFT_4_PENALTY=0.207669
PRIMER_RIGHT_4_PENALTY=1.181943
PRIMER_INTERNAL_4_PENALTY=3.883941
PRIMER_LEFT_4_SEQUENCE=TATTTGCAGAGATCCGCATG
PRIMER_RIGHT_4_SEQUENCE=TGTGTGTGTACACACGTGTGC
PRIMER_INTERNAL_4_SEQUENCE=TTCTCACCATCACAACACAGTT
PRIMER_LEFT_4=81,20
PRIMER_RIGHT_4=162,21
PRIMER_INTERNAL_4=110,22
PRIMER_LEFT_4_TM=59.792
PRIMER_RIGHT_4_TM=60.182
PRIMER_INTERNAL_4_TM=58.116
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=12.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=8.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH124
SEQUENCE_TEMPLATE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGAGGCCAGGAGGGTAGGTTGATTTCAAAGCGGGCAGATGAATGTCTTATTTAGGAGTAGGATTGGGTTCACTCAAATATAAGTTTTATTTGAATTGCAAAAGGCGGCAGGTTAGATGCTTGTATACATTCTCATGTATTTNTNATTTTAAACTCTGCTGTGCCTTGGTTACATCTTTGACCTTCTTAAGAATGAGTGAAGACATTTGTTTCATTTAGCATTGGTTGAGCCTCAGATGGAGGTTGGATATTTGATTATGAAAGATGGAACGAAATGTTAG
SEQUENCE_INCLUDED_REGION=36,282
P3_COMMENT=CL is CCAGGAGGGTAGGTTGATTT
P3_COMMENT=CR is GAGGCTCAACCAATGCTAAA
P3_COMMENT=CM is AAAGCGGGCAGATGAATGTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.158903
PRIMER_LEFT_0_PENALTY=0.085951
PRIMER_RIGHT_0_PENALTY=0.072951
PRIMER_INTERNAL_0_PENALTY=5.711025
PRIMER_LEFT_0_SEQUENCE=AACTCTGCTGTGCCTTGGTT
PRIMER_RIGHT_0_SEQUENCE=CCATCTGAGGCTCAACCAAT
PRIMER_INTERNAL_0_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA
PRIMER_LEFT_0=188,20
PRIMER_RIGHT_0=276,20
PRIMER_INTERNAL_0=227,24
PRIMER_LEFT_0_TM=59.914
PRIMER_RIGHT_0_TM=60.073
PRIMER_INTERNAL_0_TM=58.289
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=0.158903
PRIMER_LEFT_1_PENALTY=0.085951
PRIMER_RIGHT_1_PENALTY=0.072951
PRIMER_INTERNAL_1_PENALTY=5.711025
PRIMER_LEFT_1_SEQUENCE=AAACTCTGCTGTGCCTTGGT
PRIMER_RIGHT_1_SEQUENCE=CCATCTGAGGCTCAACCAAT
PRIMER_INTERNAL_1_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA
PRIMER_LEFT_1=187,20
PRIMER_RIGHT_1=276,20
PRIMER_INTERNAL_1=227,24
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.073
PRIMER_INTERNAL_1_TM=58.289
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=0.282855
PRIMER_LEFT_2_PENALTY=0.085951
PRIMER_RIGHT_2_PENALTY=0.196903
PRIMER_INTERNAL_2_PENALTY=4.212142
PRIMER_LEFT_2_SEQUENCE=AACTCTGCTGTGCCTTGGTT
PRIMER_RIGHT_2_SEQUENCE=AACCTCCATCTGAGGCTCAA
PRIMER_INTERNAL_2_SEQUENCE=GACATTTGTTTCATTTAGCATTGG
PRIMER_LEFT_2=188,20
PRIMER_RIGHT_2=281,20
PRIMER_INTERNAL_2=238,24
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=59.803
PRIMER_INTERNAL_2_TM=59.788
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=0.282855
PRIMER_LEFT_3_PENALTY=0.085951
PRIMER_RIGHT_3_PENALTY=0.196903
PRIMER_INTERNAL_3_PENALTY=4.212142
PRIMER_LEFT_3_SEQUENCE=AAACTCTGCTGTGCCTTGGT
PRIMER_RIGHT_3_SEQUENCE=AACCTCCATCTGAGGCTCAA
PRIMER_INTERNAL_3_SEQUENCE=GACATTTGTTTCATTTAGCATTGG
PRIMER_LEFT_3=187,20
PRIMER_RIGHT_3=281,20
PRIMER_INTERNAL_3=238,24
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=59.803
PRIMER_INTERNAL_3_TM=59.788
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=0.312095
PRIMER_LEFT_4_PENALTY=0.085951
PRIMER_RIGHT_4_PENALTY=0.226143
PRIMER_INTERNAL_4_PENALTY=5.711025
PRIMER_LEFT_4_SEQUENCE=AACTCTGCTGTGCCTTGGTT
PRIMER_RIGHT_4_SEQUENCE=ATCTGAGGCTCAACCAATGC
PRIMER_INTERNAL_4_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA
PRIMER_LEFT_4=188,20
PRIMER_RIGHT_4=274,20
PRIMER_INTERNAL_4=227,24
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.226
PRIMER_INTERNAL_4_TM=58.289
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=7.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH1240
SEQUENCE_TEMPLATE=GNTNGCANNCCTCCAGGTCGACTCTAGAGGATCCCTGGCCTTGCAGACATNACCAGACGACACCCAAGAAAAATGGCAGCGCAGAGCCGTGAGCATCACAGGAGGTCTCAGGGNCACGCCAAGGCCGCCTCCCAGGANAAAGCAGAACACCGGAAAAGACCTTGTGAAGACTTNTTATCTTCCCCTCCAAATGGTGTCTCTTGCTTTGGCTCTTGTCGAGTTGGGGCTTGTTGGAAGACCAGGTGAAGGCCCAGGGTNNCGAGCTCGANTTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAANTTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAG
SEQUENCE_INCLUDED_REGION=35,218
P3_COMMENT=CL is ACCAGACGACACCCAAGAAA
P3_COMMENT=CR is GGCCTTCACCTGGTCTTCC
P3_COMMENT=CM is GCAGAGCCGTGAGCATCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.176548
PRIMER_LEFT_0_PENALTY=0.029253
PRIMER_RIGHT_0_PENALTY=0.147295
PRIMER_INTERNAL_0_PENALTY=0.667795
PRIMER_LEFT_0_SEQUENCE=ACACCCAAGAAAAATGGCAG
PRIMER_RIGHT_0_SEQUENCE=TCTTTTCCGGTGTTCTGCTT
PRIMER_INTERNAL_0_SEQUENCE=CATCACAGGAGGTCTCAGGG
PRIMER_LEFT_0=59,20
PRIMER_RIGHT_0=158,20
PRIMER_INTERNAL_0=93,20
PRIMER_LEFT_0_TM=59.971
PRIMER_RIGHT_0_TM=59.853
PRIMER_INTERNAL_0_TM=60.668
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.384190
PRIMER_LEFT_1_PENALTY=0.005232
PRIMER_RIGHT_1_PENALTY=0.378958
PRIMER_INTERNAL_1_PENALTY=1.011075
PRIMER_LEFT_1_SEQUENCE=ACACCGGAAAAGACCTTGTG
PRIMER_RIGHT_1_SEQUENCE=AACTCGACAAGAGCCAAAGC
PRIMER_INTERNAL_1_SEQUENCE=CCCTCCAAATGGTGTCTCTT
PRIMER_LEFT_1=146,20
PRIMER_RIGHT_1=221,20
PRIMER_INTERNAL_1=182,20
PRIMER_LEFT_1_TM=60.005
PRIMER_RIGHT_1_TM=59.621
PRIMER_INTERNAL_1_TM=58.989
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=0.526253
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.378958
PRIMER_INTERNAL_2_PENALTY=1.011075
PRIMER_LEFT_2_SEQUENCE=AAGCAGAACACCGGAAAAGA
PRIMER_RIGHT_2_SEQUENCE=AACTCGACAAGAGCCAAAGC
PRIMER_INTERNAL_2_SEQUENCE=CCCTCCAAATGGTGTCTCTT
PRIMER_LEFT_2=139,20
PRIMER_RIGHT_2=221,20
PRIMER_INTERNAL_2=182,20
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=59.621
PRIMER_INTERNAL_2_TM=58.989
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.674472
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=0.645218
PRIMER_INTERNAL_3_PENALTY=0.667795
PRIMER_LEFT_3_SEQUENCE=ACACCCAAGAAAAATGGCAG
PRIMER_RIGHT_3_SEQUENCE=CTTTTCCGGTGTTCTGCTTT
PRIMER_INTERNAL_3_SEQUENCE=CATCACAGGAGGTCTCAGGG
PRIMER_LEFT_3=59,20
PRIMER_RIGHT_3=157,20
PRIMER_INTERNAL_3=93,20
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=59.355
PRIMER_INTERNAL_3_TM=60.668
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=0.694913
PRIMER_LEFT_4_PENALTY=0.005232
PRIMER_RIGHT_4_PENALTY=0.689681
PRIMER_INTERNAL_4_PENALTY=0.003322
PRIMER_LEFT_4_SEQUENCE=ACACCGGAAAAGACCTTGTG
PRIMER_RIGHT_4_SEQUENCE=CACCTGGTCTTCCAACAAGC
PRIMER_INTERNAL_4_SEQUENCE=GGTGTCTCTTGCTTTGGCTC
PRIMER_LEFT_4=146,20
PRIMER_RIGHT_4=244,20
PRIMER_INTERNAL_4=192,20
PRIMER_LEFT_4_TM=60.005
PRIMER_RIGHT_4_TM=60.690
PRIMER_INTERNAL_4_TM=59.997
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH1241
SEQUENCE_TEMPLATE=CTTGCATNCCTCCAGGTCGACTCTAGAGGATCCCCCTGCAGAAAGTGAGAGAAGGAGGGGACAGGACAGGATGTCTGGGCCATCCCGATGGTCCACTTGGTCTGTTCATTTGCTGAGCGGCCTTGTTCCGTGAGTNCCCTGTGGNTNTCCAGGACAACAGATGCACCATCTCTAGTGCCATTTGAAACTAAATGAANGAATGAGGAGGAAAAAAAAAAGGAAACTGGTATCTGCTGCCCAACTGATANNTGCGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
SEQUENCE_INCLUDED_REGION=35,220
P3_COMMENT=CL is GAAAGTGAGAGAAGGAGGGG
P3_COMMENT=CR is ATCAGTTGGGCAGCAGATAC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.578120
PRIMER_LEFT_0_PENALTY=0.129310
PRIMER_RIGHT_0_PENALTY=0.448810
PRIMER_INTERNAL_0_PENALTY=4.255414
PRIMER_LEFT_0_SEQUENCE=TGGTCCACTTGGTCTGTTCA
PRIMER_RIGHT_0_SEQUENCE=ATGGTGCATCTGTTGTCCTG
PRIMER_INTERNAL_0_SEQUENCE=CGGCCTTGTTCCGTGAGT
PRIMER_LEFT_0=88,20
PRIMER_RIGHT_0=168,20
PRIMER_INTERNAL_0=117,18
PRIMER_LEFT_0_TM=60.129
PRIMER_RIGHT_0_TM=59.551
PRIMER_INTERNAL_0_TM=62.255
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=0.590321
PRIMER_LEFT_1_PENALTY=0.448810
PRIMER_RIGHT_1_PENALTY=0.141511
PRIMER_INTERNAL_1_PENALTY=2.756595
PRIMER_LEFT_1_SEQUENCE=CAGGACAACAGATGCACCAT
PRIMER_RIGHT_1_SEQUENCE=GTTGGGCAGCAGATACCAGT
PRIMER_INTERNAL_1_SEQUENCE=TGAGGAGGAAAAAAAAAAGGAA
PRIMER_LEFT_1=149,20
PRIMER_RIGHT_1=241,20
PRIMER_INTERNAL_1=200,22
PRIMER_LEFT_1_TM=59.551
PRIMER_RIGHT_1_TM=60.142
PRIMER_INTERNAL_1_TM=59.243
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=31.818
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=0.725622
PRIMER_LEFT_2_PENALTY=0.448810
PRIMER_RIGHT_2_PENALTY=0.276812
PRIMER_INTERNAL_2_PENALTY=2.756595
PRIMER_LEFT_2_SEQUENCE=CAGGACAACAGATGCACCAT
PRIMER_RIGHT_2_SEQUENCE=AGTTGGGCAGCAGATACCAG
PRIMER_INTERNAL_2_SEQUENCE=TGAGGAGGAAAAAAAAAAGGAA
PRIMER_LEFT_2=149,20
PRIMER_RIGHT_2=242,20
PRIMER_INTERNAL_2=200,22
PRIMER_LEFT_2_TM=59.551
PRIMER_RIGHT_2_TM=60.277
PRIMER_INTERNAL_2_TM=59.243
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=31.818
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=0.749053
PRIMER_LEFT_3_PENALTY=0.448810
PRIMER_RIGHT_3_PENALTY=0.300243
PRIMER_INTERNAL_3_PENALTY=5.113576
PRIMER_LEFT_3_SEQUENCE=CAGGACAACAGATGCACCAT
PRIMER_RIGHT_3_SEQUENCE=GGCAGCAGATACCAGTTTCC
PRIMER_INTERNAL_3_SEQUENCE=AGTGCCATTTGAAACTAAATGAA
PRIMER_LEFT_3=149,20
PRIMER_RIGHT_3=237,20
PRIMER_INTERNAL_3=173,23
PRIMER_LEFT_3_TM=59.551
PRIMER_RIGHT_3_TM=59.700
PRIMER_INTERNAL_3_TM=57.886
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=30.435
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=0.749053
PRIMER_LEFT_4_PENALTY=0.448810
PRIMER_RIGHT_4_PENALTY=0.300243
PRIMER_INTERNAL_4_PENALTY=5.113576
PRIMER_LEFT_4_SEQUENCE=CAGGACAACAGATGCACCAT
PRIMER_RIGHT_4_SEQUENCE=GGGCAGCAGATACCAGTTTC
PRIMER_INTERNAL_4_SEQUENCE=AGTGCCATTTGAAACTAAATGAA
PRIMER_LEFT_4=149,20
PRIMER_RIGHT_4=238,20
PRIMER_INTERNAL_4=173,23
PRIMER_LEFT_4_TM=59.551
PRIMER_RIGHT_4_TM=59.700
PRIMER_INTERNAL_4_TM=57.886
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=30.435
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH1242
SEQUENCE_TEMPLATE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTTACTAGCCTGAGTNTTTTGTATATGAAATGAGAACAATGACCAATAGTGAGAAGACTTTTTTGTAGGACTGAGTNNAATAATACATGCAAAGACCCCAGCCCCTCAGTGCTCTCAGGTCAACACATATTAATTTCCTTCGTCTCTCTTTGAAGGTCAGAAAATTTGGACAGTGGCGTAGAATTACAGTGCTGTGGGCCAGTGTGCCATNTGATGGCAGAAAGCACTNNTTGGATCTCTCTGCATGT
SEQUENCE_INCLUDED_REGION=36,250
P3_COMMENT=CL is TGTATATGAAATGAGAACAATGACC
P3_COMMENT=CR is TACGCCACTGTCCAAATTTT
P3_COMMENT=CM is GTGAGAAGACTTTTTTGTAGGACTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.060277
PRIMER_LEFT_0_PENALTY=0.037936
PRIMER_RIGHT_0_PENALTY=0.022341
PRIMER_INTERNAL_0_PENALTY=0.875462
PRIMER_LEFT_0_SEQUENCE=ATACATGCAAAGACCCCAGC
PRIMER_RIGHT_0_SEQUENCE=ACGCCACTGTCCAAATTTTC
PRIMER_INTERNAL_0_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
PRIMER_LEFT_0=120,20
PRIMER_RIGHT_0=216,20
PRIMER_INTERNAL_0=141,20
PRIMER_LEFT_0_TM=59.962
PRIMER_RIGHT_0_TM=59.978
PRIMER_INTERNAL_0_TM=59.125
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=0.148328
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.110392
PRIMER_INTERNAL_1_PENALTY=0.875462
PRIMER_LEFT_1_SEQUENCE=ATACATGCAAAGACCCCAGC
PRIMER_RIGHT_1_SEQUENCE=CGCCACTGTCCAAATTTTCT
PRIMER_INTERNAL_1_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
PRIMER_LEFT_1=120,20
PRIMER_RIGHT_1=215,20
PRIMER_INTERNAL_1=141,20
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.110
PRIMER_INTERNAL_1_TM=59.125
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=0.897803
PRIMER_LEFT_2_PENALTY=0.875462
PRIMER_RIGHT_2_PENALTY=0.022341
PRIMER_INTERNAL_2_PENALTY=2.008930
PRIMER_LEFT_2_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
PRIMER_RIGHT_2_SEQUENCE=ACGCCACTGTCCAAATTTTC
PRIMER_INTERNAL_2_SEQUENCE=TTCCTTCGTCTCTCTTTGAAGG
PRIMER_LEFT_2=141,20
PRIMER_RIGHT_2=216,20
PRIMER_INTERNAL_2=172,22
PRIMER_LEFT_2_TM=59.125
PRIMER_RIGHT_2_TM=59.978
PRIMER_INTERNAL_2_TM=59.991
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=0.985854
PRIMER_LEFT_3_PENALTY=0.875462
PRIMER_RIGHT_3_PENALTY=0.110392
PRIMER_INTERNAL_3_PENALTY=2.008930
PRIMER_LEFT_3_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
PRIMER_RIGHT_3_SEQUENCE=CGCCACTGTCCAAATTTTCT
PRIMER_INTERNAL_3_SEQUENCE=TTCCTTCGTCTCTCTTTGAAGG
PRIMER_LEFT_3=141,20
PRIMER_RIGHT_3=215,20
PRIMER_INTERNAL_3=172,22
PRIMER_LEFT_3_TM=59.125
PRIMER_RIGHT_3_TM=60.110
PRIMER_INTERNAL_3_TM=59.991
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=1.048926
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=1.010990
PRIMER_INTERNAL_4_PENALTY=0.875462
PRIMER_LEFT_4_SEQUENCE=ATACATGCAAAGACCCCAGC
PRIMER_RIGHT_4_SEQUENCE=TACGCCACTGTCCAAATTTTC
PRIMER_INTERNAL_4_SEQUENCE=CCTCAGTGCTCTCAGGTCAA
PRIMER_LEFT_4=120,20
PRIMER_RIGHT_4=217,21
PRIMER_INTERNAL_4=141,20
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=59.989
PRIMER_INTERNAL_4_TM=59.125
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH1244
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGCATTTGGCTGCTCTCCAGTCATTTAATCTGGNATTTAATNACAAATAATAGTTNTATTGAGTNNCACAAAGTCAATNCTTCTTACACTCTTTTATTCAGGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC
SEQUENCE_INCLUDED_REGION=36,109
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH1245
SEQUENCE_TEMPLATE=TGCNTGCCTCCAGGTCGACTCTAGAGGATCCCCTGGCATAATACATAAGATTTAGCACTTTGAATATAATGTTTTTTCATTGAGAACATTATTTTTCTTANNAANACAACCAATATTTTTGTAATTTATAATTAATATATGACAATCCCTGTAATTCTGGTATCAGAAGATCTGGTAAAATCTAATATGTTCCGTGTGTTGTAAGATTTTNNCTTTCTGAATATAAATAANGTCATAGTATCATTGAATGCTTTTATTCATTCAAAATCAGTTATTGAAGCAATCTCTGGGTAGCTATCCAAATATCCTCTTGCAG
SEQUENCE_INCLUDED_REGION=33,283
P3_COMMENT=CL is TGGCATAATACATAAGATTTAGCA
P3_COMMENT=CR is GCTACCCAGAGATTGCTTCA
P3_COMMENT=CM is TATATGACAATCCCTGTAATTCTGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.961298
PRIMER_LEFT_0_PENALTY=5.323258
PRIMER_RIGHT_0_PENALTY=3.638040
PRIMER_INTERNAL_0_PENALTY=3.135712
PRIMER_LEFT_0_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
PRIMER_RIGHT_0_SEQUENCE=TGCAAGAGGATATTTGGATAGC
PRIMER_INTERNAL_0_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
PRIMER_LEFT_0=240,24
PRIMER_RIGHT_0=314,22
PRIMER_INTERNAL_0=268,23
PRIMER_LEFT_0_TM=58.677
PRIMER_RIGHT_0_TM=58.362
PRIMER_INTERNAL_0_TM=60.136
PRIMER_LEFT_0_GC_PERCENT=25.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=9.038563
PRIMER_LEFT_1_PENALTY=5.323258
PRIMER_RIGHT_1_PENALTY=3.715305
PRIMER_INTERNAL_1_PENALTY=3.135712
PRIMER_LEFT_1_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
PRIMER_RIGHT_1_SEQUENCE=TGCAAGAGGATATTTGGATAGCT
PRIMER_INTERNAL_1_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
PRIMER_LEFT_1=240,24
PRIMER_RIGHT_1=314,23
PRIMER_INTERNAL_1=268,23
PRIMER_LEFT_1_TM=58.677
PRIMER_RIGHT_1_TM=59.285
PRIMER_INTERNAL_1_TM=60.136
PRIMER_LEFT_1_GC_PERCENT=25.000
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_INTERNAL_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=8.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=9.038563
PRIMER_LEFT_2_PENALTY=5.323258
PRIMER_RIGHT_2_PENALTY=3.715305
PRIMER_INTERNAL_2_PENALTY=3.135712
PRIMER_LEFT_2_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
PRIMER_RIGHT_2_SEQUENCE=CTGCAAGAGGATATTTGGATAGC
PRIMER_INTERNAL_2_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
PRIMER_LEFT_2=240,24
PRIMER_RIGHT_2=315,23
PRIMER_INTERNAL_2=268,23
PRIMER_LEFT_2_TM=58.677
PRIMER_RIGHT_2_TM=59.285
PRIMER_INTERNAL_2_TM=60.136
PRIMER_LEFT_2_GC_PERCENT=25.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=8.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=9.267901
PRIMER_LEFT_3_PENALTY=5.552596
PRIMER_RIGHT_3_PENALTY=3.715305
PRIMER_INTERNAL_3_PENALTY=3.135712
PRIMER_LEFT_3_SEQUENCE=CATTGAATGCTTTTATTCATTCAAA
PRIMER_RIGHT_3_SEQUENCE=CTGCAAGAGGATATTTGGATAGC
PRIMER_INTERNAL_3_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
PRIMER_LEFT_3=241,25
PRIMER_RIGHT_3=315,23
PRIMER_INTERNAL_3=268,23
PRIMER_LEFT_3_TM=59.447
PRIMER_RIGHT_3_TM=59.285
PRIMER_INTERNAL_3_TM=60.136
PRIMER_LEFT_3_GC_PERCENT=24.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_INTERNAL_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=9.456046
PRIMER_LEFT_4_PENALTY=5.323258
PRIMER_RIGHT_4_PENALTY=4.132788
PRIMER_INTERNAL_4_PENALTY=3.135712
PRIMER_LEFT_4_SEQUENCE=TCATTGAATGCTTTTATTCATTCA
PRIMER_RIGHT_4_SEQUENCE=CTGCAAGAGGATATTTGGATAGCT
PRIMER_INTERNAL_4_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG
PRIMER_LEFT_4=240,24
PRIMER_RIGHT_4=315,24
PRIMER_INTERNAL_4=268,23
PRIMER_LEFT_4_TM=58.677
PRIMER_RIGHT_4_TM=60.133
PRIMER_INTERNAL_4_TM=60.136
PRIMER_LEFT_4_GC_PERCENT=25.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=8.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH1246
SEQUENCE_TEMPLATE=NNNTGAGNTCGCNTGCCTNCAGGTCGACTCTAGAGGATCCCTCCACTGCTGTNNTTCACACAGTNGGGNATGCTGCAACCTTAGGGCCTTTTCACTCTTCTCTCTTCATAGAACACTCCTCCCTGAGACAGCCATGTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG
SEQUENCE_INCLUDED_REGION=41,97
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH1247
SEQUENCE_TEMPLATE=NNTCCCTCCAGGTCGACTCTAGAGGNNCCCCTTACATCTCCATAAGCAGCATGTGTNTTAATTGCTGAGCCCCAGTNACCACCAGACAATCTAATCAATGTCTCCATAATTAACCTCACCAAATGGAAAGTCATAAATTTAGAACTAAAATGAAATTCTGCTCAGTCTGCATATAGAAACTGACATAGCCCAAAAGGAGACAACAGGGTNNTTACCAGAATAATTTAAGAAAACCTTGTTTGGAAGGGAAGGAAAACAAAATAATGTGATACCAAAATGACCAGATGGATCATATACATTAATCGTAGTTGCTGGGTGNCTGCTGGAAAAATATGTCTCTCACA
SEQUENCE_INCLUDED_REGION=31,313
P3_COMMENT=CL is TCTCCATAAGCAGCATGTGT
P3_COMMENT=CR is GTGAGAGACATATTTTTCCAGC
P3_COMMENT=CM is ACCACCAGACAATCTAATCAATG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.117963
PRIMER_LEFT_0_PENALTY=1.635755
PRIMER_RIGHT_0_PENALTY=0.482208
PRIMER_INTERNAL_0_PENALTY=8.377222
PRIMER_LEFT_0_SEQUENCE=TAGCCCAAAAGGAGACAACAG
PRIMER_RIGHT_0_SEQUENCE=TTTGTTTTCCTTCCCTTCCA
PRIMER_INTERNAL_0_SEQUENCE=CCAGAATAATTTAAGAAAACCTTGTT
PRIMER_LEFT_0=185,21
PRIMER_RIGHT_0=259,20
PRIMER_INTERNAL_0=214,26
PRIMER_LEFT_0_TM=59.364
PRIMER_RIGHT_0_TM=59.518
PRIMER_INTERNAL_0_TM=57.623
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=26.923
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=2.130360
PRIMER_LEFT_1_PENALTY=1.458762
PRIMER_RIGHT_1_PENALTY=0.671597
PRIMER_INTERNAL_1_PENALTY=1.269110
PRIMER_LEFT_1_SEQUENCE=CCTCACCAAATGGAAAGTCA
PRIMER_RIGHT_1_SEQUENCE=CTGTTGTCTCCTTTTGGGCT
PRIMER_INTERNAL_1_SEQUENCE=TGAAATTCTGCTCAGTCTGCA
PRIMER_LEFT_1=113,20
PRIMER_RIGHT_1=205,20
PRIMER_INTERNAL_1=150,21
PRIMER_LEFT_1_TM=58.541
PRIMER_RIGHT_1_TM=59.328
PRIMER_INTERNAL_1_TM=59.731
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=2.130608
PRIMER_LEFT_2_PENALTY=1.648400
PRIMER_RIGHT_2_PENALTY=0.482208
PRIMER_INTERNAL_2_PENALTY=8.377222
PRIMER_LEFT_2_SEQUENCE=TAGCCCAAAAGGAGACAACA
PRIMER_RIGHT_2_SEQUENCE=TTTGTTTTCCTTCCCTTCCA
PRIMER_INTERNAL_2_SEQUENCE=CCAGAATAATTTAAGAAAACCTTGTT
PRIMER_LEFT_2=185,20
PRIMER_RIGHT_2=259,20
PRIMER_INTERNAL_2=214,26
PRIMER_LEFT_2_TM=58.352
PRIMER_RIGHT_2_TM=59.518
PRIMER_INTERNAL_2_TM=57.623
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=26.923
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=2.160491
PRIMER_LEFT_3_PENALTY=1.678283
PRIMER_RIGHT_3_PENALTY=0.482208
PRIMER_INTERNAL_3_PENALTY=9.300837
PRIMER_LEFT_3_SEQUENCE=CTGACATAGCCCAAAAGGAGA
PRIMER_RIGHT_3_SEQUENCE=TGTTTTCCTTCCCTTCCAAA
PRIMER_INTERNAL_3_SEQUENCE=TTACCAGAATAATTTAAGAAAACCTTG
PRIMER_LEFT_3=179,21
PRIMER_RIGHT_3=257,20
PRIMER_INTERNAL_3=211,27
PRIMER_LEFT_3_TM=59.322
PRIMER_RIGHT_3_TM=59.518
PRIMER_INTERNAL_3_TM=57.699
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=25.926
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=2.160491
PRIMER_LEFT_4_PENALTY=1.678283
PRIMER_RIGHT_4_PENALTY=0.482208
PRIMER_INTERNAL_4_PENALTY=8.800552
PRIMER_LEFT_4_SEQUENCE=CTGACATAGCCCAAAAGGAGA
PRIMER_RIGHT_4_SEQUENCE=TTGTTTTCCTTCCCTTCCAA
PRIMER_INTERNAL_4_SEQUENCE=ACCAGAATAATTTAAGAAAACCTTGT
PRIMER_LEFT_4=179,21
PRIMER_RIGHT_4=258,20
PRIMER_INTERNAL_4=213,26
PRIMER_LEFT_4_TM=59.322
PRIMER_RIGHT_4_TM=59.518
PRIMER_INTERNAL_4_TM=57.199
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=26.923
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH1248
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCTTCTCTTTCTCAGCCTGTTTTCCTCATCTGTAAAATAAAAGGGTTGTATCAGACAATTTCTATTATCTTTCTCTAACTCATTTTGTCTTATATAGGTATAAATGAAAATACAAGAAATTGTCTTTAATCTGGACAAGTTCTATAGGACACACCGTGCTGAATCTTACAGAGAAGNACTTGGGTTAGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA
SEQUENCE_INCLUDED_REGION=36,191
P3_COMMENT=CL is CAGCCTGTTTTCCTCATCTG
P3_COMMENT=CR is TCAGCACGGTGTGTCCTATA
P3_COMMENT=CM is AAAATAAAAGGGTTGTATCAGACAA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.100570
PRIMER_LEFT_0_PENALTY=6.265639
PRIMER_RIGHT_0_PENALTY=0.834931
PRIMER_INTERNAL_0_PENALTY=6.056317
PRIMER_LEFT_0_SEQUENCE=AAAAGGGTTGTATCAGACAATTTC
PRIMER_RIGHT_0_SEQUENCE=TTCAGCACGGTGTGTCCTAT
PRIMER_INTERNAL_0_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
PRIMER_LEFT_0=75,24
PRIMER_RIGHT_0=199,20
PRIMER_INTERNAL_0=149,26
PRIMER_LEFT_0_TM=57.734
PRIMER_RIGHT_0_TM=59.165
PRIMER_INTERNAL_0_TM=59.944
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=30.769
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=125
PRIMER_PAIR_1_PENALTY=7.210709
PRIMER_LEFT_1_PENALTY=6.375778
PRIMER_RIGHT_1_PENALTY=0.834931
PRIMER_INTERNAL_1_PENALTY=6.056317
PRIMER_LEFT_1_SEQUENCE=AAAAGGGTTGTATCAGACAATTTCT
PRIMER_RIGHT_1_SEQUENCE=TTCAGCACGGTGTGTCCTAT
PRIMER_INTERNAL_1_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
PRIMER_LEFT_1=75,25
PRIMER_RIGHT_1=199,20
PRIMER_INTERNAL_1=149,26
PRIMER_LEFT_1_TM=58.624
PRIMER_RIGHT_1_TM=59.165
PRIMER_INTERNAL_1_TM=59.944
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=30.769
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=125
PRIMER_PAIR_2_PENALTY=7.443073
PRIMER_LEFT_2_PENALTY=6.608142
PRIMER_RIGHT_2_PENALTY=0.834931
PRIMER_INTERNAL_2_PENALTY=6.056317
PRIMER_LEFT_2_SEQUENCE=AAAGGGTTGTATCAGACAATTTCT
PRIMER_RIGHT_2_SEQUENCE=TTCAGCACGGTGTGTCCTAT
PRIMER_INTERNAL_2_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
PRIMER_LEFT_2=76,24
PRIMER_RIGHT_2=199,20
PRIMER_INTERNAL_2=149,26
PRIMER_LEFT_2_TM=57.392
PRIMER_RIGHT_2_TM=59.165
PRIMER_INTERNAL_2_TM=59.944
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=30.769
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=124
PRIMER_PAIR_3_PENALTY=7.443073
PRIMER_LEFT_3_PENALTY=6.608142
PRIMER_RIGHT_3_PENALTY=0.834931
PRIMER_INTERNAL_3_PENALTY=6.056317
PRIMER_LEFT_3_SEQUENCE=AAAGGGTTGTATCAGACAATTTCT
PRIMER_RIGHT_3_SEQUENCE=ATTCAGCACGGTGTGTCCTA
PRIMER_INTERNAL_3_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA
PRIMER_LEFT_3=76,24
PRIMER_RIGHT_3=200,20
PRIMER_INTERNAL_3=149,26
PRIMER_LEFT_3_TM=57.392
PRIMER_RIGHT_3_TM=59.165
PRIMER_INTERNAL_3_TM=59.944
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=125
PRIMER_PAIR_4_PENALTY=7.768938
PRIMER_LEFT_4_PENALTY=1.558232
PRIMER_RIGHT_4_PENALTY=6.210707
PRIMER_INTERNAL_4_PENALTY=6.265639
PRIMER_LEFT_4_SEQUENCE=CAGCCTGTTTTCCTCATCTG
PRIMER_RIGHT_4_SEQUENCE=TGTCCAGATTAAAGACAATTTCTTG
PRIMER_INTERNAL_4_SEQUENCE=AAAAGGGTTGTATCAGACAATTTC
PRIMER_LEFT_4=49,20
PRIMER_RIGHT_4=173,25
PRIMER_INTERNAL_4=75,24
PRIMER_LEFT_4_TM=58.442
PRIMER_RIGHT_4_TM=58.789
PRIMER_INTERNAL_4_TM=57.734
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=32.000
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=125
=
SEQUENCE_ID=MH1250
SEQUENCE_TEMPLATE=CTCTTCACTCCAGGTCGACTCTAGAGGATCCCTCTGTTATNTATAATTTNATTTGGAAAACTTAAACCTAGACAGGGATCATAACTTGGAAATCATCAAGTTGAAAAAAGAATCATAGATGGCCTCTGGAATTTNGTTCAAAAGAANACTGGAAACTGAAAATTNNGGGACTCATTTAAGTCATGTATTCAGTTTTTTAAAATATCTTTAAGANCNNTANNNNNNCCAGAGAAAAGTTGTCTATCTGTATGTGTGTGNGNGTGTGTGTTTGTGTGTGCACAC
SEQUENCE_INCLUDED_REGION=32,250
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.972563
PRIMER_LEFT_0_PENALTY=4.066464
PRIMER_RIGHT_0_PENALTY=1.906099
PRIMER_INTERNAL_0_PENALTY=4.134716
PRIMER_LEFT_0_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
PRIMER_RIGHT_0_SEQUENCE=TCCAGAGGCCATCTATGATTC
PRIMER_INTERNAL_0_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
PRIMER_LEFT_0=53,24
PRIMER_RIGHT_0=129,21
PRIMER_INTERNAL_0=85,24
PRIMER_LEFT_0_TM=59.934
PRIMER_RIGHT_0_TM=59.094
PRIMER_INTERNAL_0_TM=59.865
PRIMER_LEFT_0_GC_PERCENT=41.667
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=25.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=6.126660
PRIMER_LEFT_1_PENALTY=4.066464
PRIMER_RIGHT_1_PENALTY=2.060196
PRIMER_INTERNAL_1_PENALTY=4.359729
PRIMER_LEFT_1_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
PRIMER_RIGHT_1_SEQUENCE=TCCAGAGGCCATCTATGATTCT
PRIMER_INTERNAL_1_SEQUENCE=TTGGAAATCATCAAGTTGAAAAA
PRIMER_LEFT_1=53,24
PRIMER_RIGHT_1=129,22
PRIMER_INTERNAL_1=85,23
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=60.060
PRIMER_INTERNAL_1_TM=58.640
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=26.087
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=6.135990
PRIMER_LEFT_2_PENALTY=4.066464
PRIMER_RIGHT_2_PENALTY=2.069526
PRIMER_INTERNAL_2_PENALTY=4.134716
PRIMER_LEFT_2_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
PRIMER_RIGHT_2_SEQUENCE=AAATTCCAGAGGCCATCTATGA
PRIMER_INTERNAL_2_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
PRIMER_LEFT_2=53,24
PRIMER_RIGHT_2=133,22
PRIMER_INTERNAL_2=85,24
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=59.930
PRIMER_INTERNAL_2_TM=59.865
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_INTERNAL_2_GC_PERCENT=25.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=6.456280
PRIMER_LEFT_3_PENALTY=4.066464
PRIMER_RIGHT_3_PENALTY=2.389816
PRIMER_INTERNAL_3_PENALTY=4.134716
PRIMER_LEFT_3_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
PRIMER_RIGHT_3_SEQUENCE=TTCCAGAGGCCATCTATGATT
PRIMER_INTERNAL_3_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
PRIMER_LEFT_3=53,24
PRIMER_RIGHT_3=130,21
PRIMER_INTERNAL_3=85,24
PRIMER_LEFT_3_TM=59.934
PRIMER_RIGHT_3_TM=58.610
PRIMER_INTERNAL_3_TM=59.865
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=25.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=6.456280
PRIMER_LEFT_4_PENALTY=4.066464
PRIMER_RIGHT_4_PENALTY=2.389816
PRIMER_INTERNAL_4_PENALTY=4.134716
PRIMER_LEFT_4_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG
PRIMER_RIGHT_4_SEQUENCE=AATTCCAGAGGCCATCTATGA
PRIMER_INTERNAL_4_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA
PRIMER_LEFT_4=53,24
PRIMER_RIGHT_4=132,21
PRIMER_INTERNAL_4=85,24
PRIMER_LEFT_4_TM=59.934
PRIMER_RIGHT_4_TM=58.610
PRIMER_INTERNAL_4_TM=59.865
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=25.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH1251
SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAAGGAGTNNGCCACATGGATGATTGGAGAAAGTGCATCCTAGGCAGAGGANACAGCAAGTGCAAATGCCTTAAGATAGCGTAAGTCTAGGGAGTGTGAGATTTGCAAGGATGCAGGGTAATTGAAGAAAAGTGAGTAAAGGAGCCAGAAAGAGATGGATGTAGAGAGGTATCCAGTGGCCAGACCATACATAGCCCTGTGAGCCTTTTTAAGGAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACA
SEQUENCE_INCLUDED_REGION=36,218
P3_COMMENT=CL is CCACATGGATGATTGGAGAA
P3_COMMENT=CR is TAAAAAGGCTCACAGGGCTA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.078512
PRIMER_LEFT_0_PENALTY=0.026532
PRIMER_RIGHT_0_PENALTY=0.051981
PRIMER_INTERNAL_0_PENALTY=0.014163
PRIMER_LEFT_0_SEQUENCE=AGTGCATCCTAGGCAGAGGA
PRIMER_RIGHT_0_SEQUENCE=CTGCATCCTTGCAAATCTCA
PRIMER_INTERNAL_0_SEQUENCE=CAGCAAGTGCAAATGCCTTA
PRIMER_LEFT_0=69,20
PRIMER_RIGHT_0=153,20
PRIMER_INTERNAL_0=91,20
PRIMER_LEFT_0_TM=59.973
PRIMER_RIGHT_0_TM=59.948
PRIMER_INTERNAL_0_TM=60.014
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=0.221130
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.194598
PRIMER_INTERNAL_1_PENALTY=0.014163
PRIMER_LEFT_1_SEQUENCE=AGTGCATCCTAGGCAGAGGA
PRIMER_RIGHT_1_SEQUENCE=GCATCCTTGCAAATCTCACA
PRIMER_INTERNAL_1_SEQUENCE=CAGCAAGTGCAAATGCCTTA
PRIMER_LEFT_1=69,20
PRIMER_RIGHT_1=151,20
PRIMER_INTERNAL_1=91,20
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.805
PRIMER_INTERNAL_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.221130
PRIMER_LEFT_2_PENALTY=0.026532
PRIMER_RIGHT_2_PENALTY=0.194598
PRIMER_INTERNAL_2_PENALTY=0.014163
PRIMER_LEFT_2_SEQUENCE=AGTGCATCCTAGGCAGAGGA
PRIMER_RIGHT_2_SEQUENCE=TGCATCCTTGCAAATCTCAC
PRIMER_INTERNAL_2_SEQUENCE=CAGCAAGTGCAAATGCCTTA
PRIMER_LEFT_2=69,20
PRIMER_RIGHT_2=152,20
PRIMER_INTERNAL_2=91,20
PRIMER_LEFT_2_TM=59.973
PRIMER_RIGHT_2_TM=59.805
PRIMER_INTERNAL_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=84
PRIMER_PAIR_3_PENALTY=0.242322
PRIMER_LEFT_3_PENALTY=0.051981
PRIMER_RIGHT_3_PENALTY=0.190341
PRIMER_INTERNAL_3_PENALTY=1.033644
PRIMER_LEFT_3_SEQUENCE=TGAGATTTGCAAGGATGCAG
PRIMER_RIGHT_3_SEQUENCE=ATGGTCTGGCCACTGGATAC
PRIMER_INTERNAL_3_SEQUENCE=AGGAGCCAGAAAGAGATGGA
PRIMER_LEFT_3=134,20
PRIMER_RIGHT_3=225,20
PRIMER_INTERNAL_3=177,20
PRIMER_LEFT_3_TM=59.948
PRIMER_RIGHT_3_TM=59.810
PRIMER_INTERNAL_3_TM=58.966
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=0.242322
PRIMER_LEFT_4_PENALTY=0.051981
PRIMER_RIGHT_4_PENALTY=0.190341
PRIMER_INTERNAL_4_PENALTY=1.033644
PRIMER_LEFT_4_SEQUENCE=TGAGATTTGCAAGGATGCAG
PRIMER_RIGHT_4_SEQUENCE=GTATGGTCTGGCCACTGGAT
PRIMER_INTERNAL_4_SEQUENCE=AGGAGCCAGAAAGAGATGGA
PRIMER_LEFT_4=134,20
PRIMER_RIGHT_4=227,20
PRIMER_INTERNAL_4=177,20
PRIMER_LEFT_4_TM=59.948
PRIMER_RIGHT_4_TM=59.810
PRIMER_INTERNAL_4_TM=58.966
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=94
=
SEQUENCE_ID=MH1254
SEQUENCE_TEMPLATE=CTTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGCCTACCTCTCACTCCCTCTAGCCAGCNTACCTCTGGCCAGTNTCCTGGCAGGCCATGCATTTCTAGCCCTCTCCACCTGTGCATGTCCTGTCTCCTCCCCGTTCTTCTCTGCCAAACTAATTTCTGTCCTCCTCATGTGGAGCCTGGACCCCCACCTTGAGGCCAGTGCCCATTTTCTGTGGCCCCATTGCACCTTCTAGCAGAACCCTTAAACTGCACACAGCAAGGGGTACNGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
SEQUENCE_INCLUDED_REGION=35,231
P3_COMMENT=CL is TACCTCTCACTCCCTCTAGCC
P3_COMMENT=CR is GGTTCTGCTAGAAGGTGCAA
P3_COMMENT=CM is GGCAGGCCATGCATTTCTAG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.388917
PRIMER_LEFT_0_PENALTY=0.216148
PRIMER_RIGHT_0_PENALTY=0.172769
PRIMER_INTERNAL_0_PENALTY=0.707509
PRIMER_LEFT_0_SEQUENCE=GGCCTACCTCTCACTCCCTC
PRIMER_RIGHT_0_SEQUENCE=GAGGAGACAGGACATGCACA
PRIMER_INTERNAL_0_SEQUENCE=CCATGCATTTCTAGCCCTCT
PRIMER_LEFT_0=37,20
PRIMER_RIGHT_0=135,20
PRIMER_INTERNAL_0=91,20
PRIMER_LEFT_0_TM=60.216
PRIMER_RIGHT_0_TM=59.827
PRIMER_INTERNAL_0_TM=59.292
PRIMER_LEFT_0_GC_PERCENT=65.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=0.469491
PRIMER_LEFT_1_PENALTY=0.296722
PRIMER_RIGHT_1_PENALTY=0.172769
PRIMER_INTERNAL_1_PENALTY=0.707509
PRIMER_LEFT_1_SEQUENCE=TCTCACTCCCTCTAGCCAGC
PRIMER_RIGHT_1_SEQUENCE=GAGGAGACAGGACATGCACA
PRIMER_INTERNAL_1_SEQUENCE=CCATGCATTTCTAGCCCTCT
PRIMER_LEFT_1=45,20
PRIMER_RIGHT_1=135,20
PRIMER_INTERNAL_1=91,20
PRIMER_LEFT_1_TM=59.703
PRIMER_RIGHT_1_TM=59.827
PRIMER_INTERNAL_1_TM=59.292
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.478476
PRIMER_LEFT_2_PENALTY=0.216148
PRIMER_RIGHT_2_PENALTY=0.262328
PRIMER_INTERNAL_2_PENALTY=0.707509
PRIMER_LEFT_2_SEQUENCE=GGCCTACCTCTCACTCCCTC
PRIMER_RIGHT_2_SEQUENCE=AGACAGGACATGCACAGGTG
PRIMER_INTERNAL_2_SEQUENCE=CCATGCATTTCTAGCCCTCT
PRIMER_LEFT_2=37,20
PRIMER_RIGHT_2=131,20
PRIMER_INTERNAL_2=91,20
PRIMER_LEFT_2_TM=60.216
PRIMER_RIGHT_2_TM=59.738
PRIMER_INTERNAL_2_TM=59.292
PRIMER_LEFT_2_GC_PERCENT=65.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=0.559051
PRIMER_LEFT_3_PENALTY=0.296722
PRIMER_RIGHT_3_PENALTY=0.262328
PRIMER_INTERNAL_3_PENALTY=0.707509
PRIMER_LEFT_3_SEQUENCE=TCTCACTCCCTCTAGCCAGC
PRIMER_RIGHT_3_SEQUENCE=AGACAGGACATGCACAGGTG
PRIMER_INTERNAL_3_SEQUENCE=CCATGCATTTCTAGCCCTCT
PRIMER_LEFT_3=45,20
PRIMER_RIGHT_3=131,20
PRIMER_INTERNAL_3=91,20
PRIMER_LEFT_3_TM=59.703
PRIMER_RIGHT_3_TM=59.738
PRIMER_INTERNAL_3_TM=59.292
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=87
PRIMER_PAIR_4_PENALTY=0.623612
PRIMER_LEFT_4_PENALTY=0.450843
PRIMER_RIGHT_4_PENALTY=0.172769
PRIMER_INTERNAL_4_PENALTY=0.707509
PRIMER_LEFT_4_SEQUENCE=CCTCTCACTCCCTCTAGCCA
PRIMER_RIGHT_4_SEQUENCE=GAGGAGACAGGACATGCACA
PRIMER_INTERNAL_4_SEQUENCE=CCATGCATTTCTAGCCCTCT
PRIMER_LEFT_4=43,20
PRIMER_RIGHT_4=135,20
PRIMER_INTERNAL_4=91,20
PRIMER_LEFT_4_TM=59.549
PRIMER_RIGHT_4_TM=59.827
PRIMER_INTERNAL_4_TM=59.292
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH1258
SEQUENCE_TEMPLATE=CTTGNATGCCTCCAGGTCGACTCTAGAGGATCCCCTGTAATATTGGTAGGAACAAATTCTTGTGCTAGATCCTATGTNGAGTNTAGACNGGCACTCATGGAAAAAGCCTGTGAGTTCCTCCCAGCAATGACTACTGGGACTTTGTACTAAACCATATCCTTTCAAGGGGCAATTATCAGCTTTCTATTGGNTATTAATTGAAACTACTGCAAAGACTAAAGGACATCAAATAATCTTGAAACTTGAAATACCCATGTTGTCATGGTGATGTAAGAGAAACATTTTAATGGGGATAGCAGTGCCCAGAAAAGTTTCATAATAAAAATGGGAAGTGGTTTATAGGGAATCATGCTGTGTGGG
SEQUENCE_INCLUDED_REGION=35,325
P3_COMMENT=CL is GAACAAATTCTTGTGCTAGATCC
P3_COMMENT=CR is ACAGCATGATTCCCTATAAACC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.555836
PRIMER_LEFT_0_PENALTY=0.077340
PRIMER_RIGHT_0_PENALTY=0.478496
PRIMER_INTERNAL_0_PENALTY=5.566536
PRIMER_LEFT_0_SEQUENCE=TTAATGGGGATAGCAGTGCC
PRIMER_RIGHT_0_SEQUENCE=CCACACAGCATGATTCCCTA
PRIMER_INTERNAL_0_SEQUENCE=TTTCATAATAAAAATGGGAAGTGG
PRIMER_LEFT_0=283,20
PRIMER_RIGHT_0=358,20
PRIMER_INTERNAL_0=311,24
PRIMER_LEFT_0_TM=59.923
PRIMER_RIGHT_0_TM=59.522
PRIMER_INTERNAL_0_TM=58.433
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=0.666384
PRIMER_LEFT_1_PENALTY=0.628294
PRIMER_RIGHT_1_PENALTY=0.038090
PRIMER_INTERNAL_1_PENALTY=3.604943
PRIMER_LEFT_1_SEQUENCE=AAAGCCTGTGAGTTCCTCCC
PRIMER_RIGHT_1_SEQUENCE=GCTGATAATTGCCCCTTGAA
PRIMER_INTERNAL_1_SEQUENCE=AGCAATGACTACTGGGACTTTG
PRIMER_LEFT_1=102,20
PRIMER_RIGHT_1=179,20
PRIMER_INTERNAL_1=122,22
PRIMER_LEFT_1_TM=60.628
PRIMER_RIGHT_1_TM=60.038
PRIMER_INTERNAL_1_TM=58.395
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=0.813808
PRIMER_LEFT_2_PENALTY=0.775718
PRIMER_RIGHT_2_PENALTY=0.038090
PRIMER_INTERNAL_2_PENALTY=0.339231
PRIMER_LEFT_2_SEQUENCE=GCACTCATGGAAAAAGCCTG
PRIMER_RIGHT_2_SEQUENCE=GCTGATAATTGCCCCTTGAA
PRIMER_INTERNAL_2_SEQUENCE=GAGTTCCTCCCAGCAATGAC
PRIMER_LEFT_2=90,20
PRIMER_RIGHT_2=179,20
PRIMER_INTERNAL_2=111,20
PRIMER_LEFT_2_TM=60.776
PRIMER_RIGHT_2_TM=60.038
PRIMER_INTERNAL_2_TM=59.661
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=0.881630
PRIMER_LEFT_3_PENALTY=0.775718
PRIMER_RIGHT_3_PENALTY=0.105912
PRIMER_INTERNAL_3_PENALTY=0.339231
PRIMER_LEFT_3_SEQUENCE=GCACTCATGGAAAAAGCCTG
PRIMER_RIGHT_3_SEQUENCE=TTGCCCCTTGAAAGGATATG
PRIMER_INTERNAL_3_SEQUENCE=GAGTTCCTCCCAGCAATGAC
PRIMER_LEFT_3=90,20
PRIMER_RIGHT_3=171,20
PRIMER_INTERNAL_3=111,20
PRIMER_LEFT_3_TM=60.776
PRIMER_RIGHT_3_TM=59.894
PRIMER_INTERNAL_3_TM=59.661
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=0.928040
PRIMER_LEFT_4_PENALTY=0.775718
PRIMER_RIGHT_4_PENALTY=0.152322
PRIMER_INTERNAL_4_PENALTY=0.339231
PRIMER_LEFT_4_SEQUENCE=GCACTCATGGAAAAAGCCTG
PRIMER_RIGHT_4_SEQUENCE=GCCCCTTGAAAGGATATGGT
PRIMER_INTERNAL_4_SEQUENCE=GAGTTCCTCCCAGCAATGAC
PRIMER_LEFT_4=90,20
PRIMER_RIGHT_4=169,20
PRIMER_INTERNAL_4=111,20
PRIMER_LEFT_4_TM=60.776
PRIMER_RIGHT_4_TM=60.152
PRIMER_INTERNAL_4_TM=59.661
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH1259
SEQUENCE_TEMPLATE=CTNGCANCCCTCCAGGTCGACTCTAGAGGATCCCCCTGTAAATATAACATGCTCACTAGAAATATTTTGTTTAAGGGGAAAACAAATATTGCATACCTTTTTGGGTTGTTCATTCAAAAAACCAAAGTAGTGTCTGTTTAGGGAGGGATAAGTNGATTTNCTATTAAAGGGNCTTTTCAACAAAAACAGTAGAAAGGAGACCTTTCATTTGAACAAGTCTTTATAACAAACAACACTTTGTTCTCTTAGTNNGAAAGACTATTTNCTTCTCAGGNACCAATACCTAACANCTAAAANCAGAAGATAAAGATGTATTGTTCGGAGCA
SEQUENCE_INCLUDED_REGION=35,291
P3_COMMENT=CL is TGCATACCTTTTTGGGTTGT
P3_COMMENT=CR is CGAACAATACATCTTTATCTTCTG
P3_COMMENT=CM is CATTCAAAAAACCAAAGTAGTGTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.912298
PRIMER_LEFT_0_PENALTY=2.515919
PRIMER_RIGHT_0_PENALTY=4.396379
PRIMER_INTERNAL_0_PENALTY=1.175168
PRIMER_LEFT_0_SEQUENCE=AAGGGGAAAACAAATATTGCA
PRIMER_RIGHT_0_SEQUENCE=TCCCTCCCTAAACAGACACTACTT
PRIMER_INTERNAL_0_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
PRIMER_LEFT_0=72,21
PRIMER_RIGHT_0=147,24
PRIMER_INTERNAL_0=95,21
PRIMER_LEFT_0_TM=58.484
PRIMER_RIGHT_0_TM=59.604
PRIMER_INTERNAL_0_TM=59.825
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=45.833
PRIMER_INTERNAL_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=7.134140
PRIMER_LEFT_1_PENALTY=2.515919
PRIMER_RIGHT_1_PENALTY=4.618221
PRIMER_INTERNAL_1_PENALTY=1.175168
PRIMER_LEFT_1_SEQUENCE=AAGGGGAAAACAAATATTGCA
PRIMER_RIGHT_1_SEQUENCE=CTTATCCCTCCCTAAACAGACA
PRIMER_INTERNAL_1_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
PRIMER_LEFT_1=72,21
PRIMER_RIGHT_1=151,22
PRIMER_INTERNAL_1=95,21
PRIMER_LEFT_1_TM=58.484
PRIMER_RIGHT_1_TM=57.382
PRIMER_INTERNAL_1_TM=59.825
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=7.167059
PRIMER_LEFT_2_PENALTY=2.515919
PRIMER_RIGHT_2_PENALTY=4.651140
PRIMER_INTERNAL_2_PENALTY=1.175168
PRIMER_LEFT_2_SEQUENCE=AAGGGGAAAACAAATATTGCA
PRIMER_RIGHT_2_SEQUENCE=TCCCTCCCTAAACAGACACTACT
PRIMER_INTERNAL_2_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
PRIMER_LEFT_2=72,21
PRIMER_RIGHT_2=147,23
PRIMER_INTERNAL_2=95,21
PRIMER_LEFT_2_TM=58.484
PRIMER_RIGHT_2_TM=58.349
PRIMER_INTERNAL_2_TM=59.825
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_INTERNAL_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=7.170905
PRIMER_LEFT_3_PENALTY=2.515919
PRIMER_RIGHT_3_PENALTY=4.654986
PRIMER_INTERNAL_3_PENALTY=1.175168
PRIMER_LEFT_3_SEQUENCE=AAGGGGAAAACAAATATTGCA
PRIMER_RIGHT_3_SEQUENCE=TCCCTCCCTAAACAGACACTAC
PRIMER_INTERNAL_3_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
PRIMER_LEFT_3=72,21
PRIMER_RIGHT_3=147,22
PRIMER_INTERNAL_3=95,21
PRIMER_LEFT_3_TM=58.484
PRIMER_RIGHT_3_TM=57.345
PRIMER_INTERNAL_3_TM=59.825
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=5.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=7.279827
PRIMER_LEFT_4_PENALTY=2.515919
PRIMER_RIGHT_4_PENALTY=4.763908
PRIMER_INTERNAL_4_PENALTY=1.175168
PRIMER_LEFT_4_SEQUENCE=AAGGGGAAAACAAATATTGCA
PRIMER_RIGHT_4_SEQUENCE=TTATCCCTCCCTAAACAGACAC
PRIMER_INTERNAL_4_SEQUENCE=CCTTTTTGGGTTGTTCATTCA
PRIMER_LEFT_4=72,21
PRIMER_RIGHT_4=150,22
PRIMER_INTERNAL_4=95,21
PRIMER_LEFT_4_TM=58.484
PRIMER_RIGHT_4_TM=57.236
PRIMER_INTERNAL_4_TM=59.825
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH1262
SEQUENCE_TEMPLATE=CTTGCATGACTCCAGGTCGACTCTAGAGGATCCCCCTGTNCTGGGCAACCTGAAAAGCATTTTCCCAAGAGAGTNGTAAGACTGACTTCTTTCTTTGTNAGGTCTCTGCTTAAATGACATTTACTCACTGAGGCCTTCCTTGATTTCCTGACATTAAAAACTCACCTACAGCCCCCTCTGCTACCTATCCTAGTTTATCTCCCTTTTTCCAGAGTATATATCACCATCTGATAATACATTTTGACTTTCTTCTTTGTTATGTCTGTTTCTGCCAACTGGAAGGCAGAAGCTCTTTTTGACCAGAGTTGTATTCTCAGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC
SEQUENCE_INCLUDED_REGION=35,281
P3_COMMENT=CL is AACCTGAAAAGCATTTTCCC
P3_COMMENT=CR is AAAAAGAGCTTCTGCCTTCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.761875
PRIMER_LEFT_0_PENALTY=0.045749
PRIMER_RIGHT_0_PENALTY=0.716126
PRIMER_INTERNAL_0_PENALTY=7.723451
PRIMER_LEFT_0_SEQUENCE=CCTGAAAAGCATTTTCCCAA
PRIMER_RIGHT_0_SEQUENCE=AATCAAGGAAGGCCTCAGTG
PRIMER_INTERNAL_0_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
PRIMER_LEFT_0=48,20
PRIMER_RIGHT_0=144,20
PRIMER_INTERNAL_0=100,25
PRIMER_LEFT_0_TM=60.046
PRIMER_RIGHT_0_TM=59.284
PRIMER_INTERNAL_0_TM=57.277
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=36.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=11.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=1.170173
PRIMER_LEFT_1_PENALTY=0.454047
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_INTERNAL_1_PENALTY=7.723451
PRIMER_LEFT_1_SEQUENCE=ACCTGAAAAGCATTTTCCCA
PRIMER_RIGHT_1_SEQUENCE=AATCAAGGAAGGCCTCAGTG
PRIMER_INTERNAL_1_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
PRIMER_LEFT_1=47,20
PRIMER_RIGHT_1=144,20
PRIMER_INTERNAL_1=100,25
PRIMER_LEFT_1_TM=59.546
PRIMER_RIGHT_1_TM=59.284
PRIMER_INTERNAL_1_TM=57.277
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=36.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=11.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=98
PRIMER_PAIR_2_PENALTY=1.184523
PRIMER_LEFT_2_PENALTY=0.045749
PRIMER_RIGHT_2_PENALTY=1.138774
PRIMER_INTERNAL_2_PENALTY=7.723451
PRIMER_LEFT_2_SEQUENCE=CCTGAAAAGCATTTTCCCAA
PRIMER_RIGHT_2_SEQUENCE=GAAATCAAGGAAGGCCTCAG
PRIMER_INTERNAL_2_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
PRIMER_LEFT_2=48,20
PRIMER_RIGHT_2=146,20
PRIMER_INTERNAL_2=100,25
PRIMER_LEFT_2_TM=60.046
PRIMER_RIGHT_2_TM=58.861
PRIMER_INTERNAL_2_TM=57.277
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=36.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=11.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=7.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=1.341210
PRIMER_LEFT_3_PENALTY=0.045749
PRIMER_RIGHT_3_PENALTY=1.295461
PRIMER_INTERNAL_3_PENALTY=7.723451
PRIMER_LEFT_3_SEQUENCE=CCTGAAAAGCATTTTCCCAA
PRIMER_RIGHT_3_SEQUENCE=GAAATCAAGGAAGGCCTCAGT
PRIMER_INTERNAL_3_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
PRIMER_LEFT_3=48,20
PRIMER_RIGHT_3=146,21
PRIMER_INTERNAL_3=100,25
PRIMER_LEFT_3_TM=60.046
PRIMER_RIGHT_3_TM=59.705
PRIMER_INTERNAL_3_TM=57.277
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=36.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=11.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=1.505752
PRIMER_LEFT_4_PENALTY=0.045749
PRIMER_RIGHT_4_PENALTY=1.460004
PRIMER_INTERNAL_4_PENALTY=7.723451
PRIMER_LEFT_4_SEQUENCE=CCTGAAAAGCATTTTCCCAA
PRIMER_RIGHT_4_SEQUENCE=GGAAATCAAGGAAGGCCTCA
PRIMER_INTERNAL_4_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT
PRIMER_LEFT_4=48,20
PRIMER_RIGHT_4=147,20
PRIMER_INTERNAL_4=100,25
PRIMER_LEFT_4_TM=60.046
PRIMER_RIGHT_4_TM=61.460
PRIMER_INTERNAL_4_TM=57.277
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=36.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=11.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH1267
SEQUENCE_TEMPLATE=NTNCATNCTCCAGGTCGACTCTAGAGGATCCCCCTGGGAGCAGGTGTGTNAGGCCCCCACACACTGTAAAGCGATCCCCTTCCAAGGNGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAGCCTGGGNNNCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGANTCGGCCAACGCGCGGGGAGAGGCGNTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
SEQUENCE_INCLUDED_REGION=33,52
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1270
SEQUENCE_TEMPLATE=CTTGCATGCNTNCAGGTCGACTCTAGAGGATCCCTCCTNGACCTNGGGCTCCGGGACTGTGTACTCGGCGCCCCAGTCGAAGCGGCGCACCGGGGCNTGTACTTGTAAAACGCGAACTGCAGCGGCGTGTCGNGCTTCTGCGNGTGCAGGCGCGTGTCGNAGCGCANACCACCCCACCCAGCGCCGNGCCGGGCCTCCCGGNACNCCATCACCCTCAGCACCGGCGCCCGGAACAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGNCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTG
SEQUENCE_INCLUDED_REGION=34,201
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH1276
SEQUENCE_TEMPLATE=GTTNATNNCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCAGATGGGGAGTTGGTGCATGAGTNCACAGAGGCTGGAAGNGGGNGTTGGGGAGTNGGTGGACGGTGGAACCCAGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
SEQUENCE_INCLUDED_REGION=34,83
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH128
SEQUENCE_TEMPLATE=GCTTGCNTGCCTGCAGGACGTCNACTGTAGAGGATCCCCTTNAGGGTTCCNATTTAGTGCTTTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATNAATTGCGTTGCGCNCANTGGCCGCTTTCCAGG
SEQUENCE_INCLUDED_REGION=39,26
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH1284
SEQUENCE_TEMPLATE=CTTCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTCTCCAGCAGTNGGGAACTCATACATTACACGGCAGCCCATTTCACAGAAGGATGACTTCGGGCTCACATCTCTTAGCAACTAACTGCCTAGCCCTTCCTGGCAGAGAAGTCTCAGGTGGACTCTGTCTAAGTGGGCAACACAGGAATGCACATCAGTNCACACCCTCCTAAGGAGAAGCATCTTCCCACACCTCCATTGCAGGGCTTCTAACAATTTTNTCCCCCTCCTGGTAGGCGATGCCTGGCTCCTCTCCTAAGAAATCAAAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGNGCCGGAAGCATAAAGTGTAAA
SEQUENCE_INCLUDED_REGION=34,271
P3_COMMENT=CL is GGGCTCACATCTCTTAGCAA
P3_COMMENT=CR is TGATTTCTTAGGAGAGGAGCC
P3_COMMENT=CM is TAACTGCCTAGCCCTTCCTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.211322
PRIMER_LEFT_0_PENALTY=0.014419
PRIMER_RIGHT_0_PENALTY=0.196903
PRIMER_INTERNAL_0_PENALTY=0.128434
PRIMER_LEFT_0_SEQUENCE=GGCAGAGAAGTCTCAGGTGG
PRIMER_RIGHT_0_SEQUENCE=TGTGGGAAGATGCTTCTCCT
PRIMER_INTERNAL_0_SEQUENCE=GCAACACAGGAATGCACATC
PRIMER_LEFT_0=137,20
PRIMER_RIGHT_0=227,20
PRIMER_INTERNAL_0=172,20
PRIMER_LEFT_0_TM=59.986
PRIMER_RIGHT_0_TM=59.803
PRIMER_INTERNAL_0_TM=60.128
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=0.235264
PRIMER_LEFT_1_PENALTY=0.014419
PRIMER_RIGHT_1_PENALTY=0.220845
PRIMER_INTERNAL_1_PENALTY=0.128434
PRIMER_LEFT_1_SEQUENCE=GGCAGAGAAGTCTCAGGTGG
PRIMER_RIGHT_1_SEQUENCE=AATGGAGGTGTGGGAAGATG
PRIMER_INTERNAL_1_SEQUENCE=GCAACACAGGAATGCACATC
PRIMER_LEFT_1=137,20
PRIMER_RIGHT_1=235,20
PRIMER_INTERNAL_1=172,20
PRIMER_LEFT_1_TM=59.986
PRIMER_RIGHT_1_TM=59.779
PRIMER_INTERNAL_1_TM=60.128
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=0.235264
PRIMER_LEFT_2_PENALTY=0.014419
PRIMER_RIGHT_2_PENALTY=0.220845
PRIMER_INTERNAL_2_PENALTY=0.128434
PRIMER_LEFT_2_SEQUENCE=GGCAGAGAAGTCTCAGGTGG
PRIMER_RIGHT_2_SEQUENCE=CAATGGAGGTGTGGGAAGAT
PRIMER_INTERNAL_2_SEQUENCE=GCAACACAGGAATGCACATC
PRIMER_LEFT_2=137,20
PRIMER_RIGHT_2=236,20
PRIMER_INTERNAL_2=172,20
PRIMER_LEFT_2_TM=59.986
PRIMER_RIGHT_2_TM=59.779
PRIMER_INTERNAL_2_TM=60.128
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=0.324229
PRIMER_LEFT_3_PENALTY=0.127326
PRIMER_RIGHT_3_PENALTY=0.196903
PRIMER_INTERNAL_3_PENALTY=0.128434
PRIMER_LEFT_3_SEQUENCE=CCTGGCAGAGAAGTCTCAGG
PRIMER_RIGHT_3_SEQUENCE=TGTGGGAAGATGCTTCTCCT
PRIMER_INTERNAL_3_SEQUENCE=GCAACACAGGAATGCACATC
PRIMER_LEFT_3=134,20
PRIMER_RIGHT_3=227,20
PRIMER_INTERNAL_3=172,20
PRIMER_LEFT_3_TM=60.127
PRIMER_RIGHT_3_TM=59.803
PRIMER_INTERNAL_3_TM=60.128
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=0.341939
PRIMER_LEFT_4_PENALTY=0.128434
PRIMER_RIGHT_4_PENALTY=0.213506
PRIMER_INTERNAL_4_PENALTY=0.196903
PRIMER_LEFT_4_SEQUENCE=GCAACACAGGAATGCACATC
PRIMER_RIGHT_4_SEQUENCE=GTTAGAAGCCCTGCAATGGA
PRIMER_INTERNAL_4_SEQUENCE=AGGAGAAGCATCTTCCCACA
PRIMER_LEFT_4=172,20
PRIMER_RIGHT_4=249,20
PRIMER_INTERNAL_4=208,20
PRIMER_LEFT_4_TM=60.128
PRIMER_RIGHT_4_TM=60.214
PRIMER_INTERNAL_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH1290
SEQUENCE_TEMPLATE=CTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTGCGCGTNGGTNGAATTGTTAATAAATTACANNCACTCTNTTCTTTGAATGTTTCTGCTCCTTTTGTATATAAGTTCCANNGGTTTCCCATGATGGATATTAATACCTACTGAAAAGAGTGACAATTGTGCTCATTGCTAAGCTGAAAAAATGTAATCCGTCTATCTGTNTTCTCTGCAGTACATAACACTAGAAAATCTAATCACCTTGAAATTGTTTGAAAGCAAGCACAAGAAAATTCATGGATGTTCACTATAGAATGAGCACAATAGAGCACCTCAGGGTNNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCC
SEQUENCE_INCLUDED_REGION=34,283
P3_COMMENT=CL is TTCTTTGAATGTTTCTGCTCC
P3_COMMENT=CR is CATCCATGAATTTTCTTGTGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.122709
PRIMER_LEFT_0_PENALTY=2.728489
PRIMER_RIGHT_0_PENALTY=1.394220
PRIMER_INTERNAL_0_PENALTY=2.920989
PRIMER_LEFT_0_SEQUENCE=CCTTGAAATTGTTTGAAAGCAA
PRIMER_RIGHT_0_SEQUENCE=TGAGGTGCTCTATTGTGCTCA
PRIMER_INTERNAL_0_SEQUENCE=GCACAAGAAAATTCATGGATG
PRIMER_LEFT_0=241,22
PRIMER_RIGHT_0=316,21
PRIMER_INTERNAL_0=263,21
PRIMER_LEFT_0_TM=59.272
PRIMER_RIGHT_0_TM=59.606
PRIMER_INTERNAL_0_TM=58.079
PRIMER_LEFT_0_GC_PERCENT=31.818
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=4.291738
PRIMER_LEFT_1_PENALTY=1.232835
PRIMER_RIGHT_1_PENALTY=3.058902
PRIMER_INTERNAL_1_PENALTY=9.021019
PRIMER_LEFT_1_SEQUENCE=TGTGCTCATTGCTAAGCTGAA
PRIMER_RIGHT_1_SEQUENCE=TGCTTTCAAACAATTTCAAGG
PRIMER_INTERNAL_1_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA
PRIMER_LEFT_1=162,21
PRIMER_RIGHT_1=261,21
PRIMER_INTERNAL_1=206,27
PRIMER_LEFT_1_TM=59.767
PRIMER_RIGHT_1_TM=57.941
PRIMER_INTERNAL_1_TM=57.979
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=33.333
PRIMER_INTERNAL_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=4.424336
PRIMER_LEFT_2_PENALTY=3.030116
PRIMER_RIGHT_2_PENALTY=1.394220
PRIMER_INTERNAL_2_PENALTY=2.920989
PRIMER_LEFT_2_SEQUENCE=ACCTTGAAATTGTTTGAAAGCAA
PRIMER_RIGHT_2_SEQUENCE=TGAGGTGCTCTATTGTGCTCA
PRIMER_INTERNAL_2_SEQUENCE=GCACAAGAAAATTCATGGATG
PRIMER_LEFT_2=240,23
PRIMER_RIGHT_2=316,21
PRIMER_INTERNAL_2=263,21
PRIMER_LEFT_2_TM=60.030
PRIMER_RIGHT_2_TM=59.606
PRIMER_INTERNAL_2_TM=58.079
PRIMER_LEFT_2_GC_PERCENT=30.435
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=4.445603
PRIMER_LEFT_3_PENALTY=1.232835
PRIMER_RIGHT_3_PENALTY=3.212768
PRIMER_INTERNAL_3_PENALTY=9.021019
PRIMER_LEFT_3_SEQUENCE=TGTGCTCATTGCTAAGCTGAA
PRIMER_RIGHT_3_SEQUENCE=GCTTTCAAACAATTTCAAGGTG
PRIMER_INTERNAL_3_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA
PRIMER_LEFT_3=162,21
PRIMER_RIGHT_3=260,22
PRIMER_INTERNAL_3=206,27
PRIMER_LEFT_3_TM=59.767
PRIMER_RIGHT_3_TM=58.787
PRIMER_INTERNAL_3_TM=57.979
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=36.364
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=4.445603
PRIMER_LEFT_4_PENALTY=1.232835
PRIMER_RIGHT_4_PENALTY=3.212768
PRIMER_INTERNAL_4_PENALTY=9.021019
PRIMER_LEFT_4_SEQUENCE=TGTGCTCATTGCTAAGCTGAA
PRIMER_RIGHT_4_SEQUENCE=TGCTTTCAAACAATTTCAAGGT
PRIMER_INTERNAL_4_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA
PRIMER_LEFT_4=162,21
PRIMER_RIGHT_4=261,22
PRIMER_INTERNAL_4=206,27
PRIMER_LEFT_4_TM=59.767
PRIMER_RIGHT_4_TM=58.787
PRIMER_INTERNAL_4_TM=57.979
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=31.818
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH1291
SEQUENCE_TEMPLATE=GCTTGCANNCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAAGTNAGAATCAGGAAATATGATTTTNCTATCATGAAGGGAATTTAAATTGAGCGTGATATCTATTGCTTATAATTTTAAATTAGGCTTAATATTANGTTTTTAAAAAGTGCTAAACCACCCCTTTTAAAAACGTTTACATCTGATTCACAGAACTAAACTAAAATGTATTTGTTACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCNAGTCGGGAAACC
SEQUENCE_INCLUDED_REGION=36,185
P3_COMMENT=CL is GAAGGGAATTTAAATTGAGCG
P3_COMMENT=CR is CATTTTAGTTTAGTTCTGTGAATCA
P3_COMMENT=CM is GCAATAGATATCACGCTCAATTT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=13.351711
PRIMER_LEFT_0_PENALTY=5.547770
PRIMER_RIGHT_0_PENALTY=7.803941
PRIMER_INTERNAL_0_PENALTY=6.402746
PRIMER_LEFT_0_SEQUENCE=AATTGAGCGTGATATCTATTGCTT
PRIMER_RIGHT_0_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
PRIMER_INTERNAL_0_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
PRIMER_LEFT_0=88,24
PRIMER_RIGHT_0=187,25
PRIMER_INTERNAL_0=139,24
PRIMER_LEFT_0_TM=58.452
PRIMER_RIGHT_0_TM=57.196
PRIMER_INTERNAL_0_TM=57.597
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=28.000
PRIMER_INTERNAL_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=7.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=13.627595
PRIMER_LEFT_1_PENALTY=5.823654
PRIMER_RIGHT_1_PENALTY=7.803941
PRIMER_INTERNAL_1_PENALTY=6.402746
PRIMER_LEFT_1_SEQUENCE=ATTGAGCGTGATATCTATTGCTT
PRIMER_RIGHT_1_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
PRIMER_INTERNAL_1_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
PRIMER_LEFT_1=89,23
PRIMER_RIGHT_1=187,25
PRIMER_INTERNAL_1=139,24
PRIMER_LEFT_1_TM=57.176
PRIMER_RIGHT_1_TM=57.196
PRIMER_INTERNAL_1_TM=57.597
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=28.000
PRIMER_INTERNAL_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=13.627595
PRIMER_LEFT_2_PENALTY=5.823654
PRIMER_RIGHT_2_PENALTY=7.803941
PRIMER_INTERNAL_2_PENALTY=6.402746
PRIMER_LEFT_2_SEQUENCE=AATTGAGCGTGATATCTATTGCT
PRIMER_RIGHT_2_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
PRIMER_INTERNAL_2_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
PRIMER_LEFT_2=88,23
PRIMER_RIGHT_2=187,25
PRIMER_INTERNAL_2=139,24
PRIMER_LEFT_2_TM=57.176
PRIMER_RIGHT_2_TM=57.196
PRIMER_INTERNAL_2_TM=57.597
PRIMER_LEFT_2_GC_PERCENT=34.783
PRIMER_RIGHT_2_GC_PERCENT=28.000
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=10.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=7.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=14.293279
PRIMER_LEFT_3_PENALTY=6.489338
PRIMER_RIGHT_3_PENALTY=7.803941
PRIMER_INTERNAL_3_PENALTY=6.402746
PRIMER_LEFT_3_SEQUENCE=AATTGAGCGTGATATCTATTGCTTA
PRIMER_RIGHT_3_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
PRIMER_INTERNAL_3_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
PRIMER_LEFT_3=88,25
PRIMER_RIGHT_3=187,25
PRIMER_INTERNAL_3=139,24
PRIMER_LEFT_3_TM=58.511
PRIMER_RIGHT_3_TM=57.196
PRIMER_INTERNAL_3_TM=57.597
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=28.000
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=7.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=14.523720
PRIMER_LEFT_4_PENALTY=6.719779
PRIMER_RIGHT_4_PENALTY=7.803941
PRIMER_INTERNAL_4_PENALTY=6.402746
PRIMER_LEFT_4_SEQUENCE=TTGAGCGTGATATCTATTGCTTAT
PRIMER_RIGHT_4_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG
PRIMER_INTERNAL_4_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC
PRIMER_LEFT_4=90,24
PRIMER_RIGHT_4=187,25
PRIMER_INTERNAL_4=139,24
PRIMER_LEFT_4_TM=57.280
PRIMER_RIGHT_4_TM=57.196
PRIMER_INTERNAL_4_TM=57.597
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=28.000
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=7.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH1292
SEQUENCE_TEMPLATE=CTNCANCCCTCCAGGTCGACTCTAGAGGATCCCTAGTNCNGANTNAGGTNGTNCCTTCAGAGCNGAGAAAGGGAGACAGGGTTACTCAGACCGGCAGTGTCACCAGTGGATGATTCCCCCNAAACACACTTCTCAACAGACTCAAGTGAGATGAAGTATACACNACAGGCAACTCGTGAATATTAACTACTTNTCTATGCTCATGCAGAATAACTTAAAATAATAAAGANTTCAACACAAATGATCAGTGACATGAATGGGAAGAGACCAATTATGATTAAACACAGCAATACTGTGAGATGAGTCAGGGGTACCGAGCTCGNATTCGTAATCATGGTCATAGCTGTTTCCNGTGTG
SEQUENCE_INCLUDED_REGION=33,275
P3_COMMENT=CL is GGAGACAGGGTTACTCAGACC
P3_COMMENT=CR is TTGGTCTCTTCCCATTCATG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.289927
PRIMER_LEFT_0_PENALTY=0.426379
PRIMER_RIGHT_0_PENALTY=4.863548
PRIMER_INTERNAL_0_PENALTY=0.184073
PRIMER_LEFT_0_SEQUENCE=GACAGGGTTACTCAGACCGG
PRIMER_RIGHT_0_SEQUENCE=TCATCTCACTTGAGTCTGTTGAGA
PRIMER_INTERNAL_0_SEQUENCE=GTCACCAGTGGATGATTCCC
PRIMER_LEFT_0=74,20
PRIMER_RIGHT_0=153,24
PRIMER_INTERNAL_0=98,20
PRIMER_LEFT_0_TM=59.574
PRIMER_RIGHT_0_TM=59.136
PRIMER_INTERNAL_0_TM=60.184
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=41.667
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=7.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=11.1000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=5.564178
PRIMER_LEFT_1_PENALTY=0.426379
PRIMER_RIGHT_1_PENALTY=5.137799
PRIMER_INTERNAL_1_PENALTY=0.184073
PRIMER_LEFT_1_SEQUENCE=GACAGGGTTACTCAGACCGG
PRIMER_RIGHT_1_SEQUENCE=TCTCACTTGAGTCTGTTGAGAAGTG
PRIMER_INTERNAL_1_SEQUENCE=GTCACCAGTGGATGATTCCC
PRIMER_LEFT_1=74,20
PRIMER_RIGHT_1=150,25
PRIMER_INTERNAL_1=98,20
PRIMER_LEFT_1_TM=59.574
PRIMER_RIGHT_1_TM=60.138
PRIMER_INTERNAL_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=44.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=5.574732
PRIMER_LEFT_2_PENALTY=0.711184
PRIMER_RIGHT_2_PENALTY=4.863548
PRIMER_INTERNAL_2_PENALTY=1.747386
PRIMER_LEFT_2_SEQUENCE=GGTTACTCAGACCGGCAGTG
PRIMER_RIGHT_2_SEQUENCE=TCATCTCACTTGAGTCTGTTGAGA
PRIMER_INTERNAL_2_SEQUENCE=TCACCAGTGGATGATTCCC
PRIMER_LEFT_2=79,20
PRIMER_RIGHT_2=153,24
PRIMER_INTERNAL_2=99,19
PRIMER_LEFT_2_TM=60.711
PRIMER_RIGHT_2_TM=59.136
PRIMER_INTERNAL_2_TM=59.253
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_INTERNAL_2_GC_PERCENT=52.632
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=7.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=5.626006
PRIMER_LEFT_3_PENALTY=0.184073
PRIMER_RIGHT_3_PENALTY=5.441933
PRIMER_INTERNAL_3_PENALTY=4.863548
PRIMER_LEFT_3_SEQUENCE=GTCACCAGTGGATGATTCCC
PRIMER_RIGHT_3_SEQUENCE=AGTTAATATTCACGAGTTGCCTG
PRIMER_INTERNAL_3_SEQUENCE=TCTCAACAGACTCAAGTGAGATGA
PRIMER_LEFT_3=98,20
PRIMER_RIGHT_3=187,23
PRIMER_INTERNAL_3=130,24
PRIMER_LEFT_3_TM=60.184
PRIMER_RIGHT_3_TM=57.558
PRIMER_INTERNAL_3_TM=59.136
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=5.764823
PRIMER_LEFT_4_PENALTY=0.184073
PRIMER_RIGHT_4_PENALTY=5.580751
PRIMER_INTERNAL_4_PENALTY=4.863548
PRIMER_LEFT_4_SEQUENCE=GTCACCAGTGGATGATTCCC
PRIMER_RIGHT_4_SEQUENCE=GTTAATATTCACGAGTTGCCTGT
PRIMER_INTERNAL_4_SEQUENCE=TCTCAACAGACTCAAGTGAGATGA
PRIMER_LEFT_4=98,20
PRIMER_RIGHT_4=186,23
PRIMER_INTERNAL_4=130,24
PRIMER_LEFT_4_TM=60.184
PRIMER_RIGHT_4_TM=57.419
PRIMER_INTERNAL_4_TM=59.136
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH1296
SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTTTGAAAATGTNTTAAGTNCATTATTCAAACCCAAAGTNTTAATTTTTTTAAAAAACCAATTTTCCCATCTTTCAAATAAGGGGGATGGAGCAGATGATGTTCAAGATGCTCAAAATTCTTTTTGCTCCGTGAGATCTAAAACCTGACCTTGGCATCACGTAATGACCATCACAGTCAACACTGCTCACAACATATACAATGGGATGCCTGGTATGTTTTACAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACANTTCCACACA
SEQUENCE_INCLUDED_REGION=34,223
P3_COMMENT=CL is GGATGGAGCAGATGATGTTC
P3_COMMENT=CR is ACCAGGCATCCCATTGTATA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.133274
PRIMER_LEFT_0_PENALTY=0.106742
PRIMER_RIGHT_0_PENALTY=0.026532
PRIMER_INTERNAL_0_PENALTY=1.255615
PRIMER_LEFT_0_SEQUENCE=GGGATGGAGCAGATGATGTT
PRIMER_RIGHT_0_SEQUENCE=GTGATGCCAAGGTCAGGTTT
PRIMER_INTERNAL_0_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
PRIMER_LEFT_0=116,20
PRIMER_RIGHT_0=192,20
PRIMER_INTERNAL_0=145,21
PRIMER_LEFT_0_TM=59.893
PRIMER_RIGHT_0_TM=59.973
PRIMER_INTERNAL_0_TM=59.744
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=0.218212
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.191680
PRIMER_INTERNAL_1_PENALTY=1.674451
PRIMER_LEFT_1_SEQUENCE=AAACCTGACCTTGGCATCAC
PRIMER_RIGHT_1_SEQUENCE=AAAACATACCAGGCATCCCA
PRIMER_INTERNAL_1_SEQUENCE=CCATCACAGTCAACACTGCTC
PRIMER_LEFT_1=173,20
PRIMER_RIGHT_1=253,20
PRIMER_INTERNAL_1=200,21
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=60.192
PRIMER_INTERNAL_1_TM=59.326
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=81
PRIMER_PAIR_2_PENALTY=0.276511
PRIMER_LEFT_2_PENALTY=0.249979
PRIMER_RIGHT_2_PENALTY=0.026532
PRIMER_INTERNAL_2_PENALTY=1.255615
PRIMER_LEFT_2_SEQUENCE=ATAAGGGGGATGGAGCAGAT
PRIMER_RIGHT_2_SEQUENCE=GTGATGCCAAGGTCAGGTTT
PRIMER_INTERNAL_2_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
PRIMER_LEFT_2=110,20
PRIMER_RIGHT_2=192,20
PRIMER_INTERNAL_2=145,21
PRIMER_LEFT_2_TM=59.750
PRIMER_RIGHT_2_TM=59.973
PRIMER_INTERNAL_2_TM=59.744
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.305015
PRIMER_LEFT_3_PENALTY=0.278483
PRIMER_RIGHT_3_PENALTY=0.026532
PRIMER_INTERNAL_3_PENALTY=1.255615
PRIMER_LEFT_3_SEQUENCE=AAATAAGGGGGATGGAGCAG
PRIMER_RIGHT_3_SEQUENCE=GTGATGCCAAGGTCAGGTTT
PRIMER_INTERNAL_3_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
PRIMER_LEFT_3=108,20
PRIMER_RIGHT_3=192,20
PRIMER_INTERNAL_3=145,21
PRIMER_LEFT_3_TM=60.278
PRIMER_RIGHT_3_TM=59.973
PRIMER_INTERNAL_3_TM=59.744
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=0.391116
PRIMER_LEFT_4_PENALTY=0.106742
PRIMER_RIGHT_4_PENALTY=0.284374
PRIMER_INTERNAL_4_PENALTY=1.255615
PRIMER_LEFT_4_SEQUENCE=GGGATGGAGCAGATGATGTT
PRIMER_RIGHT_4_SEQUENCE=TTACGTGATGCCAAGGTCAG
PRIMER_INTERNAL_4_SEQUENCE=CAAAATTCTTTTTGCTCCGTG
PRIMER_LEFT_4=116,20
PRIMER_RIGHT_4=196,20
PRIMER_INTERNAL_4=145,21
PRIMER_LEFT_4_TM=59.893
PRIMER_RIGHT_4_TM=59.716
PRIMER_INTERNAL_4_TM=59.744
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH1304
SEQUENCE_TEMPLATE=GNGGCATGNNCAGGTCGACTCTAGAGGATCCCCTGTGTGACAGACCCTAAAGTGGGGAACCAGTNTAAGCCATACTCAGATTNNCAAACCACCGAAACAGTGAAATATTCAGTGTATGTNATTTTAAACTGCTGATTTTAGTGGTGACTTGTTATGCAACANTAGATAACTAATACATATATAANTGGAAACGCATACACATATGCCCTTTATGTTTGGCATACTTTTCTGGAGANTTGGAAAGGTTAAATAGGAGTTTACACCATAAACATGTAAAANNNNTGCAANTTGAATGTTAGANNTTNTNCTCAGTCCTTCANCAGGGTA
SEQUENCE_INCLUDED_REGION=33,294
P3_COMMENT=CL is ACAGACCCTAAAGTGGGGAA
P3_COMMENT=CR is TTTACATGTTTATGGTGTAAACTCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.505285
PRIMER_LEFT_0_PENALTY=3.785641
PRIMER_RIGHT_0_PENALTY=2.719644
PRIMER_INTERNAL_0_PENALTY=1.231281
PRIMER_LEFT_0_SEQUENCE=AGTGGTGACTTGTTATGCAACA
PRIMER_RIGHT_0_SEQUENCE=TCCAGAAAAGTATGCCAAACA
PRIMER_INTERNAL_0_SEQUENCE=GAAACGCATACACATATGCCC
PRIMER_LEFT_0=139,22
PRIMER_RIGHT_0=232,21
PRIMER_INTERNAL_0=187,21
PRIMER_LEFT_0_TM=58.214
PRIMER_RIGHT_0_TM=58.280
PRIMER_INTERNAL_0_TM=60.231
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=6.518732
PRIMER_LEFT_1_PENALTY=3.785641
PRIMER_RIGHT_1_PENALTY=2.733091
PRIMER_INTERNAL_1_PENALTY=1.231281
PRIMER_LEFT_1_SEQUENCE=AGTGGTGACTTGTTATGCAACA
PRIMER_RIGHT_1_SEQUENCE=CTCCAGAAAAGTATGCCAAACA
PRIMER_INTERNAL_1_SEQUENCE=GAAACGCATACACATATGCCC
PRIMER_LEFT_1=139,22
PRIMER_RIGHT_1=233,22
PRIMER_INTERNAL_1=187,21
PRIMER_LEFT_1_TM=58.214
PRIMER_RIGHT_1_TM=59.267
PRIMER_INTERNAL_1_TM=60.231
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=6.619916
PRIMER_LEFT_2_PENALTY=3.900272
PRIMER_RIGHT_2_PENALTY=2.719644
PRIMER_INTERNAL_2_PENALTY=1.231281
PRIMER_LEFT_2_SEQUENCE=GTGGTGACTTGTTATGCAACA
PRIMER_RIGHT_2_SEQUENCE=TCCAGAAAAGTATGCCAAACA
PRIMER_INTERNAL_2_SEQUENCE=GAAACGCATACACATATGCCC
PRIMER_LEFT_2=140,21
PRIMER_RIGHT_2=232,21
PRIMER_INTERNAL_2=187,21
PRIMER_LEFT_2_TM=57.100
PRIMER_RIGHT_2_TM=58.280
PRIMER_INTERNAL_2_TM=60.231
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=93
PRIMER_PAIR_3_PENALTY=6.633363
PRIMER_LEFT_3_PENALTY=3.900272
PRIMER_RIGHT_3_PENALTY=2.733091
PRIMER_INTERNAL_3_PENALTY=1.231281
PRIMER_LEFT_3_SEQUENCE=GTGGTGACTTGTTATGCAACA
PRIMER_RIGHT_3_SEQUENCE=CTCCAGAAAAGTATGCCAAACA
PRIMER_INTERNAL_3_SEQUENCE=GAAACGCATACACATATGCCC
PRIMER_LEFT_3=140,21
PRIMER_RIGHT_3=233,22
PRIMER_INTERNAL_3=187,21
PRIMER_LEFT_3_TM=57.100
PRIMER_RIGHT_3_TM=59.267
PRIMER_INTERNAL_3_TM=60.231
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_INTERNAL_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=7.101617
PRIMER_LEFT_4_PENALTY=4.381973
PRIMER_RIGHT_4_PENALTY=2.719644
PRIMER_INTERNAL_4_PENALTY=1.231281
PRIMER_LEFT_4_SEQUENCE=TTAGTGGTGACTTGTTATGCAACA
PRIMER_RIGHT_4_SEQUENCE=TCCAGAAAAGTATGCCAAACA
PRIMER_INTERNAL_4_SEQUENCE=GAAACGCATACACATATGCCC
PRIMER_LEFT_4=137,24
PRIMER_RIGHT_4=232,21
PRIMER_INTERNAL_4=187,21
PRIMER_LEFT_4_TM=59.618
PRIMER_RIGHT_4_TM=58.280
PRIMER_INTERNAL_4_TM=60.231
PRIMER_LEFT_4_GC_PERCENT=37.500
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH1306
SEQUENCE_TEMPLATE=NTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTCTGTACCTCACTTCTGATTTCTGTATGTCACTTTCCTTTTGTTGTNTATAAATTTGTTCTGACCACGTGGCATCCCTGGNGTNNCTCTGNATCTGCTGTGATTCTGGAGACTGCCAGATCTGCTAATCATTTTTTTTTCTTGGTCCGTTAAACTCTGTTAAATTTAATTTGTCCGAAGTTTTCTTTTAACAGGGGAAAGCATCGTATTCATAATGGCAGTAAGAACCCGATATAATTCCCTAGAAAAAAGTTATTCTCCTTTAACCCAGTAACCCTACTTCTATGACTCTATTTATGGGNAAAATCCAAAGTA
SEQUENCE_INCLUDED_REGION=35,318
P3_COMMENT=CL is TCTGATTTCTGTATGTCACTTTCC
P3_COMMENT=CR is TCGGGTTCTTACTGCCATTA
P3_COMMENT=CM is TATAAATTTGTTCTGACCACGTGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.155134
PRIMER_LEFT_0_PENALTY=1.087947
PRIMER_RIGHT_0_PENALTY=0.067187
PRIMER_INTERNAL_0_PENALTY=1.915462
PRIMER_LEFT_0_SEQUENCE=TGGAGACTGCCAGATCTGC
PRIMER_RIGHT_0_SEQUENCE=CGATGCTTTCCCCTGTTAAA
PRIMER_INTERNAL_0_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
PRIMER_LEFT_0=144,19
PRIMER_RIGHT_0=243,20
PRIMER_INTERNAL_0=166,21
PRIMER_LEFT_0_TM=60.088
PRIMER_RIGHT_0_TM=60.067
PRIMER_INTERNAL_0_TM=59.085
PRIMER_LEFT_0_GC_PERCENT=57.895
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=1.192410
PRIMER_LEFT_1_PENALTY=1.125223
PRIMER_RIGHT_1_PENALTY=0.067187
PRIMER_INTERNAL_1_PENALTY=1.915462
PRIMER_LEFT_1_SEQUENCE=TGGAGACTGCCAGATCTGCT
PRIMER_RIGHT_1_SEQUENCE=CGATGCTTTCCCCTGTTAAA
PRIMER_INTERNAL_1_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
PRIMER_LEFT_1=144,20
PRIMER_RIGHT_1=243,20
PRIMER_INTERNAL_1=166,21
PRIMER_LEFT_1_TM=61.125
PRIMER_RIGHT_1_TM=60.067
PRIMER_INTERNAL_1_TM=59.085
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=1.478710
PRIMER_LEFT_2_PENALTY=1.411523
PRIMER_RIGHT_2_PENALTY=0.067187
PRIMER_INTERNAL_2_PENALTY=1.915462
PRIMER_LEFT_2_SEQUENCE=GGAGACTGCCAGATCTGCTAA
PRIMER_RIGHT_2_SEQUENCE=CGATGCTTTCCCCTGTTAAA
PRIMER_INTERNAL_2_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
PRIMER_LEFT_2=145,21
PRIMER_RIGHT_2=243,20
PRIMER_INTERNAL_2=166,21
PRIMER_LEFT_2_TM=59.588
PRIMER_RIGHT_2_TM=60.067
PRIMER_INTERNAL_2_TM=59.085
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=1.842580
PRIMER_LEFT_3_PENALTY=1.411523
PRIMER_RIGHT_3_PENALTY=0.431057
PRIMER_INTERNAL_3_PENALTY=1.915462
PRIMER_LEFT_3_SEQUENCE=GGAGACTGCCAGATCTGCTAA
PRIMER_RIGHT_3_SEQUENCE=ACGATGCTTTCCCCTGTTAA
PRIMER_INTERNAL_3_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
PRIMER_LEFT_3=145,21
PRIMER_RIGHT_3=244,20
PRIMER_INTERNAL_3=166,21
PRIMER_LEFT_3_TM=59.588
PRIMER_RIGHT_3_TM=59.569
PRIMER_INTERNAL_3_TM=59.085
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=1.938080
PRIMER_LEFT_4_PENALTY=1.870893
PRIMER_RIGHT_4_PENALTY=0.067187
PRIMER_INTERNAL_4_PENALTY=1.915462
PRIMER_LEFT_4_SEQUENCE=GGAGACTGCCAGATCTGCTA
PRIMER_RIGHT_4_SEQUENCE=CGATGCTTTCCCCTGTTAAA
PRIMER_INTERNAL_4_SEQUENCE=TCATTTTTTTTTCTTGGTCCG
PRIMER_LEFT_4=145,20
PRIMER_RIGHT_4=243,20
PRIMER_INTERNAL_4=166,21
PRIMER_LEFT_4_TM=58.129
PRIMER_RIGHT_4_TM=60.067
PRIMER_INTERNAL_4_TM=59.085
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH1307
SEQUENCE_TEMPLATE=CTTCATTCTCCAGGTCGACTCTAGAGGATCCCCTGTCTAACACAGCCTATCGATATAGTNTNNTAGATCTATTTTCTGCAGGACTATGCATAAAATCCTGGGAGAGTTCATAGAAAGTNCATCTACCTAAGGCAGAAGAACAGGCAGCACCTTCAAGTNCTTCTGTTACATGGGCAATAGGATGNAGAAGTGAGCAGCATGAGCAAAGTTATTGAAGTATGCTAGAAAATAATGCTCAAGGTCTTAAGCAATTTAATAGGTAAAGGTTAGNGTNCATGNGGGTGGTAGAAGGTCAGANTAGAGTNNTAGANGNGGCATGGTAAANGNCTGGAGCAGTGG
SEQUENCE_INCLUDED_REGION=33,306
P3_COMMENT=CL is ATCTATTTTCTGCAGGACTATGC
P3_COMMENT=CR is CCTTTACCTATTAAATTGCTTAAGA
P3_COMMENT=CM is TAAAATCCTGGGAGAGTTCATAGA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.257145
PRIMER_LEFT_0_PENALTY=0.003322
PRIMER_RIGHT_0_PENALTY=0.253823
PRIMER_INTERNAL_0_PENALTY=2.526059
PRIMER_LEFT_0_SEQUENCE=GAAGAACAGGCAGCACCTTC
PRIMER_RIGHT_0_SEQUENCE=AACTTTGCTCATGCTGCTCA
PRIMER_INTERNAL_0_SEQUENCE=TTCTGTTACATGGGCAATAGGA
PRIMER_LEFT_0=134,20
PRIMER_RIGHT_0=209,20
PRIMER_INTERNAL_0=160,22
PRIMER_LEFT_0_TM=59.997
PRIMER_RIGHT_0_TM=59.746
PRIMER_INTERNAL_0_TM=59.474
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=0.808009
PRIMER_LEFT_1_PENALTY=0.003322
PRIMER_RIGHT_1_PENALTY=0.804687
PRIMER_INTERNAL_1_PENALTY=2.526059
PRIMER_LEFT_1_SEQUENCE=GAAGAACAGGCAGCACCTTC
PRIMER_RIGHT_1_SEQUENCE=ACTTTGCTCATGCTGCTCAC
PRIMER_INTERNAL_1_SEQUENCE=TTCTGTTACATGGGCAATAGGA
PRIMER_LEFT_1=134,20
PRIMER_RIGHT_1=208,20
PRIMER_INTERNAL_1=160,22
PRIMER_LEFT_1_TM=59.997
PRIMER_RIGHT_1_TM=59.195
PRIMER_INTERNAL_1_TM=59.474
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=0.842790
PRIMER_LEFT_2_PENALTY=0.588967
PRIMER_RIGHT_2_PENALTY=0.253823
PRIMER_INTERNAL_2_PENALTY=2.526059
PRIMER_LEFT_2_SEQUENCE=CAGAAGAACAGGCAGCACCT
PRIMER_RIGHT_2_SEQUENCE=TTTGCTCATGCTGCTCACTT
PRIMER_INTERNAL_2_SEQUENCE=TTCTGTTACATGGGCAATAGGA
PRIMER_LEFT_2=132,20
PRIMER_RIGHT_2=206,20
PRIMER_INTERNAL_2=160,22
PRIMER_LEFT_2_TM=60.589
PRIMER_RIGHT_2_TM=59.746
PRIMER_INTERNAL_2_TM=59.474
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=0.842790
PRIMER_LEFT_3_PENALTY=0.588967
PRIMER_RIGHT_3_PENALTY=0.253823
PRIMER_INTERNAL_3_PENALTY=2.526059
PRIMER_LEFT_3_SEQUENCE=CAGAAGAACAGGCAGCACCT
PRIMER_RIGHT_3_SEQUENCE=AACTTTGCTCATGCTGCTCA
PRIMER_INTERNAL_3_SEQUENCE=TTCTGTTACATGGGCAATAGGA
PRIMER_LEFT_3=132,20
PRIMER_RIGHT_3=209,20
PRIMER_INTERNAL_3=160,22
PRIMER_LEFT_3_TM=60.589
PRIMER_RIGHT_3_TM=59.746
PRIMER_INTERNAL_3_TM=59.474
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=1.036230
PRIMER_LEFT_4_PENALTY=0.923358
PRIMER_RIGHT_4_PENALTY=0.112872
PRIMER_INTERNAL_4_PENALTY=2.526059
PRIMER_LEFT_4_SEQUENCE=CCTAAGGCAGAAGAACAGGC
PRIMER_RIGHT_4_SEQUENCE=TGCTCATGCTGCTCACTTCT
PRIMER_INTERNAL_4_SEQUENCE=TTCTGTTACATGGGCAATAGGA
PRIMER_LEFT_4=125,20
PRIMER_RIGHT_4=204,20
PRIMER_INTERNAL_4=160,22
PRIMER_LEFT_4_TM=59.077
PRIMER_RIGHT_4_TM=59.887
PRIMER_INTERNAL_4_TM=59.474
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH131
SEQUENCE_TEMPLATE=TCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAATAATAATTTAGTAGGATTTTTTTTTTCTTTGCCTTATGTAGACTCTGTNCTTCCTTGCAGCCCATTGAGAGCAATGATCACCATGACTGTNCCCTCACATGCAGCAGCAATGATTGTCCAAGATATACATGTTCACAGAACAAACTTTTATCATACAAGTTTTCTTTTTCTGAGAAATGTGAAGTTCCAGAATACCCTTATTTTTTTAATTTNCT
SEQUENCE_INCLUDED_REGION=31,219
P3_COMMENT=CL is TGAGAGCAATGATCACCATG
P3_COMMENT=CR is AGGGTATTCTGGAACTTCACA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.439719
PRIMER_LEFT_0_PENALTY=2.343918
PRIMER_RIGHT_0_PENALTY=1.095801
PRIMER_INTERNAL_0_PENALTY=1.895060
PRIMER_LEFT_0_SEQUENCE=CTTCCTTGCAGCCCATTG
PRIMER_RIGHT_0_SEQUENCE=ATCTTGGACAATCATTGCTGC
PRIMER_INTERNAL_0_SEQUENCE=GAGCAATGATCACCATGACTG
PRIMER_LEFT_0=84,18
PRIMER_RIGHT_0=158,21
PRIMER_INTERNAL_0=103,21
PRIMER_LEFT_0_TM=60.344
PRIMER_RIGHT_0_TM=60.096
PRIMER_INTERNAL_0_TM=59.105
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=4.194322
PRIMER_LEFT_1_PENALTY=0.163550
PRIMER_RIGHT_1_PENALTY=4.030772
PRIMER_INTERNAL_1_PENALTY=6.469560
PRIMER_LEFT_1_SEQUENCE=ATGCAGCAGCAATGATTGTC
PRIMER_RIGHT_1_SEQUENCE=TCTGGAACTTCACATTTCTCAGA
PRIMER_INTERNAL_1_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
PRIMER_LEFT_1=133,20
PRIMER_RIGHT_1=225,23
PRIMER_INTERNAL_1=162,26
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=58.969
PRIMER_INTERNAL_1_TM=59.530
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_INTERNAL_1_GC_PERCENT=30.769
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=4.225370
PRIMER_LEFT_2_PENALTY=0.194598
PRIMER_RIGHT_2_PENALTY=4.030772
PRIMER_INTERNAL_2_PENALTY=6.469560
PRIMER_LEFT_2_SEQUENCE=GCAGCAATGATTGTCCAAGA
PRIMER_RIGHT_2_SEQUENCE=TCTGGAACTTCACATTTCTCAGA
PRIMER_INTERNAL_2_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
PRIMER_LEFT_2=138,20
PRIMER_RIGHT_2=225,23
PRIMER_INTERNAL_2=162,26
PRIMER_LEFT_2_TM=59.805
PRIMER_RIGHT_2_TM=58.969
PRIMER_INTERNAL_2_TM=59.530
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_INTERNAL_2_GC_PERCENT=30.769
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=7.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=4.278266
PRIMER_LEFT_3_PENALTY=0.163550
PRIMER_RIGHT_3_PENALTY=4.114715
PRIMER_INTERNAL_3_PENALTY=6.469560
PRIMER_LEFT_3_SEQUENCE=ATGCAGCAGCAATGATTGTC
PRIMER_RIGHT_3_SEQUENCE=GGGTATTCTGGAACTTCACATTTC
PRIMER_INTERNAL_3_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
PRIMER_LEFT_3=133,20
PRIMER_RIGHT_3=231,24
PRIMER_INTERNAL_3=162,26
PRIMER_LEFT_3_TM=59.836
PRIMER_RIGHT_3_TM=60.115
PRIMER_INTERNAL_3_TM=59.530
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=4.305833
PRIMER_LEFT_4_PENALTY=0.163550
PRIMER_RIGHT_4_PENALTY=4.142283
PRIMER_INTERNAL_4_PENALTY=6.469560
PRIMER_LEFT_4_SEQUENCE=ATGCAGCAGCAATGATTGTC
PRIMER_RIGHT_4_SEQUENCE=TGGAACTTCACATTTCTCAGAAAA
PRIMER_INTERNAL_4_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA
PRIMER_LEFT_4=133,20
PRIMER_RIGHT_4=223,24
PRIMER_INTERNAL_4=162,26
PRIMER_LEFT_4_TM=59.836
PRIMER_RIGHT_4_TM=60.142
PRIMER_INTERNAL_4_TM=59.530
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=33.333
PRIMER_INTERNAL_4_GC_PERCENT=30.769
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=9.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=7.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH1311
SEQUENCE_TEMPLATE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTCTGGTNNNNGAGNNNNNATATATATTGAATAATAGGTTACACCTATATATTACATCTATANTATGCCAAGAAAACCTTCAGAAGTNATACTAGAGTNNATGTGGTCTGCAGCCTTGAGCAGAAAAATTGCCTGTTAATCACTGATAGTTGAATATAAGNTTAAGATACATGCATACACAATTATACTGCCAGTTTCACATATGAGCCTCTTTGTGTNCAATANAGATTGTGATGAAAATGCACAGGGTACCGAGCTCGAATTNGTAATCATGGTCATAGCTGTTTC
SEQUENCE_INCLUDED_REGION=36,246
P3_COMMENT=CL is TGCCAAGAAAACCTTCAGAA
P3_COMMENT=CR is GGCTCATATGTGAAACTGGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.229880
PRIMER_LEFT_0_PENALTY=0.402858
PRIMER_RIGHT_0_PENALTY=1.827022
PRIMER_INTERNAL_0_PENALTY=5.069319
PRIMER_LEFT_0_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
PRIMER_RIGHT_0_SEQUENCE=GGCTCATATGTGAAACTGGC
PRIMER_INTERNAL_0_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
PRIMER_LEFT_0=145,20
PRIMER_RIGHT_0=244,20
PRIMER_INTERNAL_0=165,25
PRIMER_LEFT_0_TM=59.597
PRIMER_RIGHT_0_TM=58.173
PRIMER_INTERNAL_0_TM=60.069
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=36.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=2.450146
PRIMER_LEFT_1_PENALTY=0.402858
PRIMER_RIGHT_1_PENALTY=2.047287
PRIMER_INTERNAL_1_PENALTY=5.069319
PRIMER_LEFT_1_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
PRIMER_RIGHT_1_SEQUENCE=GGCTCATATGTGAAACTGGCA
PRIMER_INTERNAL_1_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
PRIMER_LEFT_1=145,20
PRIMER_RIGHT_1=244,21
PRIMER_INTERNAL_1=165,25
PRIMER_LEFT_1_TM=59.597
PRIMER_RIGHT_1_TM=61.047
PRIMER_INTERNAL_1_TM=60.069
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=36.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=3.009711
PRIMER_LEFT_2_PENALTY=0.402858
PRIMER_RIGHT_2_PENALTY=2.606853
PRIMER_INTERNAL_2_PENALTY=5.069319
PRIMER_LEFT_2_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
PRIMER_RIGHT_2_SEQUENCE=GCTCATATGTGAAACTGGCAG
PRIMER_INTERNAL_2_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
PRIMER_LEFT_2=145,20
PRIMER_RIGHT_2=243,21
PRIMER_INTERNAL_2=165,25
PRIMER_LEFT_2_TM=59.597
PRIMER_RIGHT_2_TM=58.393
PRIMER_INTERNAL_2_TM=60.069
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=36.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=3.125885
PRIMER_LEFT_3_PENALTY=0.402858
PRIMER_RIGHT_3_PENALTY=2.723026
PRIMER_INTERNAL_3_PENALTY=5.069319
PRIMER_LEFT_3_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
PRIMER_RIGHT_3_SEQUENCE=GCTCATATGTGAAACTGGCA
PRIMER_INTERNAL_3_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
PRIMER_LEFT_3=145,20
PRIMER_RIGHT_3=243,20
PRIMER_INTERNAL_3=165,25
PRIMER_LEFT_3_TM=59.597
PRIMER_RIGHT_3_TM=57.277
PRIMER_INTERNAL_3_TM=60.069
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=36.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=3.132696
PRIMER_LEFT_4_PENALTY=0.402858
PRIMER_RIGHT_4_PENALTY=2.729838
PRIMER_INTERNAL_4_PENALTY=5.069319
PRIMER_LEFT_4_SEQUENCE=GCAGCCTTGAGCAGAAAAAT
PRIMER_RIGHT_4_SEQUENCE=GCTCATATGTGAAACTGGCAGT
PRIMER_INTERNAL_4_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA
PRIMER_LEFT_4=145,20
PRIMER_RIGHT_4=243,22
PRIMER_INTERNAL_4=165,25
PRIMER_LEFT_4_TM=59.597
PRIMER_RIGHT_4_TM=59.270
PRIMER_INTERNAL_4_TM=60.069
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_INTERNAL_4_GC_PERCENT=36.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=7.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH1313
SEQUENCE_TEMPLATE=GNTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCTCAGCCAGANACCTGACTTTCTCTCCCTCACAACCACCCATGACCAAGCCTGTCAATTTCACCTCCTATTAATAATTTCCCCTGAACCAGTCCACTCCCCTCCATCTCTATCACCACCATCCTGGGGGAGGAGACCACTACCATCTCAAACCTATGTTACACACAGTCTTCCTAACTGAAATGCTGAGCCTCTTCTAAAGCACTAGAACTTTCAAAGGCTCAGTCCTACCTTTCTCACCCTCCTGCTCAAAACCTACTCCCACTGCCATTTGTCTAAACAAAACTCTTTTATGTGGCATGAAAGGCATTTCTTGATCTATCTCC
SEQUENCE_INCLUDED_REGION=36,328
P3_COMMENT=CL is CTTTCTCTCCCTCACAACCA
P3_COMMENT=CR is ATAGATCAAGAAATGCCTTTCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.208764
PRIMER_LEFT_0_PENALTY=0.088170
PRIMER_RIGHT_0_PENALTY=0.120594
PRIMER_INTERNAL_0_PENALTY=0.168650
PRIMER_LEFT_0_SEQUENCE=TTCTCTCCCTCACAACCACC
PRIMER_RIGHT_0_SEQUENCE=TGGTGATAGAGATGGAGGGG
PRIMER_INTERNAL_0_SEQUENCE=AATTTCCCCTGAACCAGTCC
PRIMER_LEFT_0=58,20
PRIMER_RIGHT_0=155,20
PRIMER_INTERNAL_0=113,20
PRIMER_LEFT_0_TM=60.088
PRIMER_RIGHT_0_TM=59.879
PRIMER_INTERNAL_0_TM=60.169
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=10.9000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=7.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=0.237022
PRIMER_LEFT_1_PENALTY=0.156061
PRIMER_RIGHT_1_PENALTY=0.080962
PRIMER_INTERNAL_1_PENALTY=0.420772
PRIMER_LEFT_1_SEQUENCE=CTCACCCTCCTGCTCAAAAC
PRIMER_RIGHT_1_SEQUENCE=AGAAATGCCTTTCATGCCAC
PRIMER_INTERNAL_1_SEQUENCE=ACTCCCACTGCCATTTGTCT
PRIMER_LEFT_1=273,20
PRIMER_RIGHT_1=351,20
PRIMER_INTERNAL_1=295,20
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=60.081
PRIMER_INTERNAL_1_TM=59.579
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=0.256820
PRIMER_LEFT_2_PENALTY=0.088170
PRIMER_RIGHT_2_PENALTY=0.168650
PRIMER_INTERNAL_2_PENALTY=0.495173
PRIMER_LEFT_2_SEQUENCE=TTCTCTCCCTCACAACCACC
PRIMER_RIGHT_2_SEQUENCE=GGACTGGTTCAGGGGAAATT
PRIMER_INTERNAL_2_SEQUENCE=CCAAGCCTGTCAATTTCACC
PRIMER_LEFT_2=58,20
PRIMER_RIGHT_2=132,20
PRIMER_INTERNAL_2=83,20
PRIMER_LEFT_2_TM=60.088
PRIMER_RIGHT_2_TM=60.169
PRIMER_INTERNAL_2_TM=60.495
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=0.325634
PRIMER_LEFT_3_PENALTY=0.252279
PRIMER_RIGHT_3_PENALTY=0.073355
PRIMER_INTERNAL_3_PENALTY=0.407941
PRIMER_LEFT_3_SEQUENCE=ACCTTTCTCACCCTCCTGCT
PRIMER_RIGHT_3_SEQUENCE=TGCCTTTCATGCCACATAAA
PRIMER_INTERNAL_3_SEQUENCE=AAAACCTACTCCCACTGCCA
PRIMER_LEFT_3=267,20
PRIMER_RIGHT_3=346,20
PRIMER_INTERNAL_3=288,20
PRIMER_LEFT_3_TM=60.252
PRIMER_RIGHT_3_TM=60.073
PRIMER_INTERNAL_3_TM=59.592
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=0.333241
PRIMER_LEFT_4_PENALTY=0.252279
PRIMER_RIGHT_4_PENALTY=0.080962
PRIMER_INTERNAL_4_PENALTY=0.407941
PRIMER_LEFT_4_SEQUENCE=ACCTTTCTCACCCTCCTGCT
PRIMER_RIGHT_4_SEQUENCE=AGAAATGCCTTTCATGCCAC
PRIMER_INTERNAL_4_SEQUENCE=AAAACCTACTCCCACTGCCA
PRIMER_LEFT_4=267,20
PRIMER_RIGHT_4=351,20
PRIMER_INTERNAL_4=288,20
PRIMER_LEFT_4_TM=60.252
PRIMER_RIGHT_4_TM=60.081
PRIMER_INTERNAL_4_TM=59.592
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH1314
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGACATGCAGACAGTGTGTTCATTCCTTTTCATCATTGCCCTTTTAAGTCTGTTTTATCAAATACTTCTTTCCTGCCCTGGGATAAAATCTTGGGCTTTTCCAACTACTCTCAGGTATTAATGAATAGTGTGAATGAATAATCTAATTTTCCAAGCATTGAAAAGTTTAATATCCGTATCAGTTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGC
SEQUENCE_INCLUDED_REGION=36,187
P3_COMMENT=CL is CATTCCTTTTCATCATTGCC
P3_COMMENT=CR is ATACGGATATTAAACTTTTCAATGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.289165
PRIMER_LEFT_0_PENALTY=1.183253
PRIMER_RIGHT_0_PENALTY=0.105912
PRIMER_INTERNAL_0_PENALTY=0.495347
PRIMER_LEFT_0_SEQUENCE=CTGGACATGCAGACAGTGTG
PRIMER_RIGHT_0_SEQUENCE=CAAGATTTTATCCCAGGGCA
PRIMER_INTERNAL_0_SEQUENCE=TCCTTTTCATCATTGCCCTT
PRIMER_LEFT_0=36,20
PRIMER_RIGHT_0=130,20
PRIMER_INTERNAL_0=61,20
PRIMER_LEFT_0_TM=58.817
PRIMER_RIGHT_0_TM=59.894
PRIMER_INTERNAL_0_TM=59.505
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=1.307131
PRIMER_LEFT_1_PENALTY=1.201218
PRIMER_RIGHT_1_PENALTY=0.105912
PRIMER_INTERNAL_1_PENALTY=0.495347
PRIMER_LEFT_1_SEQUENCE=CTGGACATGCAGACAGTGTGT
PRIMER_RIGHT_1_SEQUENCE=CAAGATTTTATCCCAGGGCA
PRIMER_INTERNAL_1_SEQUENCE=TCCTTTTCATCATTGCCCTT
PRIMER_LEFT_1=36,21
PRIMER_RIGHT_1=130,20
PRIMER_INTERNAL_1=61,20
PRIMER_LEFT_1_TM=59.799
PRIMER_RIGHT_1_TM=59.894
PRIMER_INTERNAL_1_TM=59.505
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=1.325097
PRIMER_LEFT_2_PENALTY=1.219185
PRIMER_RIGHT_2_PENALTY=0.105912
PRIMER_INTERNAL_2_PENALTY=0.495347
PRIMER_LEFT_2_SEQUENCE=TGGACATGCAGACAGTGTGTT
PRIMER_RIGHT_2_SEQUENCE=CAAGATTTTATCCCAGGGCA
PRIMER_INTERNAL_2_SEQUENCE=TCCTTTTCATCATTGCCCTT
PRIMER_LEFT_2=37,21
PRIMER_RIGHT_2=130,20
PRIMER_INTERNAL_2=61,20
PRIMER_LEFT_2_TM=60.219
PRIMER_RIGHT_2_TM=59.894
PRIMER_INTERNAL_2_TM=59.505
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=1.363838
PRIMER_LEFT_3_PENALTY=1.257926
PRIMER_RIGHT_3_PENALTY=0.105912
PRIMER_INTERNAL_3_PENALTY=3.236418
PRIMER_LEFT_3_SEQUENCE=TGCAGACAGTGTGTTCATTCC
PRIMER_RIGHT_3_SEQUENCE=CAAGATTTTATCCCAGGGCA
PRIMER_INTERNAL_3_SEQUENCE=CATCATTGCCCTTTTAAGTCTG
PRIMER_LEFT_3=43,21
PRIMER_RIGHT_3=130,20
PRIMER_INTERNAL_3=68,22
PRIMER_LEFT_3_TM=59.742
PRIMER_RIGHT_3_TM=59.894
PRIMER_INTERNAL_3_TM=58.764
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=1.458357
PRIMER_LEFT_4_PENALTY=1.352445
PRIMER_RIGHT_4_PENALTY=0.105912
PRIMER_INTERNAL_4_PENALTY=0.495347
PRIMER_LEFT_4_SEQUENCE=TGGACATGCAGACAGTGTGT
PRIMER_RIGHT_4_SEQUENCE=CAAGATTTTATCCCAGGGCA
PRIMER_INTERNAL_4_SEQUENCE=TCCTTTTCATCATTGCCCTT
PRIMER_LEFT_4=37,20
PRIMER_RIGHT_4=130,20
PRIMER_INTERNAL_4=61,20
PRIMER_LEFT_4_TM=58.648
PRIMER_RIGHT_4_TM=59.894
PRIMER_INTERNAL_4_TM=59.505
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=94
=
SEQUENCE_ID=MH1316
SEQUENCE_TEMPLATE=NTTNCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTACGCCAACCAGGTCTCTCACCCAAAGCAAGGGGAAAAGGGCTGGATCCTGGGTGGCAAGCCAGTCCCCAAGGAAAGAATGGAGCGATGCAAATNAGAAAAAGGAACTCAAGCAGAAAGGCTCTTTTGCGGCTTCTAGTGGCAAGCCGNCCTGCATTTGCTTGTTCTGTCCCTTAGAGATGCCTCAGTTTCCTCAGCCATAAAATGGGCTGTTGTGAGGATTAAATATGANGTATAAAAATCTGTTGGATCAAGTAAGCCAAAAACATAGGGGCATTTAAAAAATTT
SEQUENCE_INCLUDED_REGION=35,288
P3_COMMENT=CL is ACCAGGTCTCTCACCCAAAG
P3_COMMENT=CR is TAATCCTCACAACAGCCCAT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.430845
PRIMER_LEFT_0_PENALTY=0.132570
PRIMER_RIGHT_0_PENALTY=0.298276
PRIMER_INTERNAL_0_PENALTY=2.003309
PRIMER_LEFT_0_SEQUENCE=CTACGCCAACCAGGTCTCTC
PRIMER_RIGHT_0_SEQUENCE=ATTCTTTCCTTGGGGACTGG
PRIMER_INTERNAL_0_SEQUENCE=AAAAGGGCTGGATCCTGG
PRIMER_LEFT_0=35,20
PRIMER_RIGHT_0=115,20
PRIMER_INTERNAL_0=70,18
PRIMER_LEFT_0_TM=59.867
PRIMER_RIGHT_0_TM=60.298
PRIMER_INTERNAL_0_TM=60.003
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.556
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=0.430845
PRIMER_LEFT_1_PENALTY=0.132570
PRIMER_RIGHT_1_PENALTY=0.298276
PRIMER_INTERNAL_1_PENALTY=2.003309
PRIMER_LEFT_1_SEQUENCE=CTACGCCAACCAGGTCTCTC
PRIMER_RIGHT_1_SEQUENCE=CCATTCTTTCCTTGGGGACT
PRIMER_INTERNAL_1_SEQUENCE=AAAAGGGCTGGATCCTGG
PRIMER_LEFT_1=35,20
PRIMER_RIGHT_1=117,20
PRIMER_INTERNAL_1=70,18
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=60.298
PRIMER_INTERNAL_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.556
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.458945
PRIMER_LEFT_2_PENALTY=0.132570
PRIMER_RIGHT_2_PENALTY=0.326376
PRIMER_INTERNAL_2_PENALTY=2.003309
PRIMER_LEFT_2_SEQUENCE=CTACGCCAACCAGGTCTCTC
PRIMER_RIGHT_2_SEQUENCE=ATTTGCATCGCTCCATTCTT
PRIMER_INTERNAL_2_SEQUENCE=AAAAGGGCTGGATCCTGG
PRIMER_LEFT_2=35,20
PRIMER_RIGHT_2=129,20
PRIMER_INTERNAL_2=70,18
PRIMER_LEFT_2_TM=59.867
PRIMER_RIGHT_2_TM=59.674
PRIMER_INTERNAL_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=55.556
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=0.479375
PRIMER_LEFT_3_PENALTY=0.326376
PRIMER_RIGHT_3_PENALTY=0.152999
PRIMER_INTERNAL_3_PENALTY=0.513718
PRIMER_LEFT_3_SEQUENCE=AAGAATGGAGCGATGCAAAT
PRIMER_RIGHT_3_SEQUENCE=GGACAGAACAAGCAAATGCA
PRIMER_INTERNAL_3_SEQUENCE=TCTTTTGCGGCTTCTAGTGG
PRIMER_LEFT_3=110,20
PRIMER_RIGHT_3=206,20
PRIMER_INTERNAL_3=157,20
PRIMER_LEFT_3_TM=59.674
PRIMER_RIGHT_3_TM=59.847
PRIMER_INTERNAL_3_TM=60.514
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=0.482436
PRIMER_LEFT_4_PENALTY=0.156061
PRIMER_RIGHT_4_PENALTY=0.326376
PRIMER_INTERNAL_4_PENALTY=2.003309
PRIMER_LEFT_4_SEQUENCE=GTCTCTCACCCAAAGCAAGG
PRIMER_RIGHT_4_SEQUENCE=ATTTGCATCGCTCCATTCTT
PRIMER_INTERNAL_4_SEQUENCE=AAAAGGGCTGGATCCTGG
PRIMER_LEFT_4=48,20
PRIMER_RIGHT_4=129,20
PRIMER_INTERNAL_4=70,18
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=59.674
PRIMER_INTERNAL_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=55.556
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH1317
SEQUENCE_TEMPLATE=CCTCTCTCTCTTTCACACTCTGAGCCTGGCTTTTCCCAGGAATCCAATAGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGG
SEQUENCE_INCLUDED_REGION=0,49
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH132
SEQUENCE_TEMPLATE=TTTCATGCTGCAGGTCGACTCTAGAGGATCCCTGACCCCTAGCCCTAGGAGCAGTGGGAAAAAAAAATACATTTTGACTGGAAGTAAACTGAGGAAACATGACATGGAGTCAGGGAGGGATTGTAGATCTTTCCCAGAGGTGTGATTTTTTAACAGACTGGCTTTTGGCGTTTGAGGAAAATCTCAACAACTCTCTGGACAGAATTTTCATAACTGTCCACTTGGCAGGGCTGATGTGTGTCTTGAAATAATTTTCAAGATTTGATTTTGAAATCTGAAATTTGATTTTATTGTATGTGCTC
SEQUENCE_INCLUDED_REGION=31,271
P3_COMMENT=CL is TAGGAGCAGTGGGAAAAAAA
P3_COMMENT=CR is TATTTCAAGACACACATCAGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.132377
PRIMER_LEFT_0_PENALTY=0.079648
PRIMER_RIGHT_0_PENALTY=0.052729
PRIMER_INTERNAL_0_PENALTY=2.023556
PRIMER_LEFT_0_SEQUENCE=ATGACATGGAGTCAGGGAGG
PRIMER_RIGHT_0_SEQUENCE=GATTTTCCTCAAACGCCAAA
PRIMER_INTERNAL_0_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
PRIMER_LEFT_0=98,20
PRIMER_RIGHT_0=182,20
PRIMER_INTERNAL_0=127,22
PRIMER_LEFT_0_TM=59.920
PRIMER_RIGHT_0_TM=60.053
PRIMER_INTERNAL_0_TM=59.976
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=0.271328
PRIMER_LEFT_1_PENALTY=0.079648
PRIMER_RIGHT_1_PENALTY=0.191680
PRIMER_INTERNAL_1_PENALTY=2.023556
PRIMER_LEFT_1_SEQUENCE=ATGACATGGAGTCAGGGAGG
PRIMER_RIGHT_1_SEQUENCE=GAGATTTTCCTCAAACGCCA
PRIMER_INTERNAL_1_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
PRIMER_LEFT_1=98,20
PRIMER_RIGHT_1=184,20
PRIMER_INTERNAL_1=127,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.192
PRIMER_INTERNAL_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=11.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=0.271328
PRIMER_LEFT_2_PENALTY=0.079648
PRIMER_RIGHT_2_PENALTY=0.191680
PRIMER_INTERNAL_2_PENALTY=2.023556
PRIMER_LEFT_2_SEQUENCE=ATGACATGGAGTCAGGGAGG
PRIMER_RIGHT_2_SEQUENCE=TGAGATTTTCCTCAAACGCC
PRIMER_INTERNAL_2_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
PRIMER_LEFT_2=98,20
PRIMER_RIGHT_2=185,20
PRIMER_INTERNAL_2=127,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=60.192
PRIMER_INTERNAL_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=0.294872
PRIMER_LEFT_3_PENALTY=0.079648
PRIMER_RIGHT_3_PENALTY=0.215225
PRIMER_INTERNAL_3_PENALTY=2.023556
PRIMER_LEFT_3_SEQUENCE=ATGACATGGAGTCAGGGAGG
PRIMER_RIGHT_3_SEQUENCE=TTTTCCTCAAACGCCAAAAG
PRIMER_INTERNAL_3_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT
PRIMER_LEFT_3=98,20
PRIMER_RIGHT_3=180,20
PRIMER_INTERNAL_3=127,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.215
PRIMER_INTERNAL_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=0.297432
PRIMER_LEFT_4_PENALTY=0.217785
PRIMER_RIGHT_4_PENALTY=0.079648
PRIMER_INTERNAL_4_PENALTY=4.656911
PRIMER_LEFT_4_SEQUENCE=CTGACCCCTAGCCCTAGGAG
PRIMER_RIGHT_4_SEQUENCE=CCTCCCTGACTCCATGTCAT
PRIMER_INTERNAL_4_SEQUENCE=TTGACTGGAAGTAAACTGAGGAAA
PRIMER_LEFT_4=31,20
PRIMER_RIGHT_4=117,20
PRIMER_INTERNAL_4=73,24
PRIMER_LEFT_4_TM=60.218
PRIMER_RIGHT_4_TM=59.920
PRIMER_INTERNAL_4_TM=59.343
PRIMER_LEFT_4_GC_PERCENT=65.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=8.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH1322
SEQUENCE_TEMPLATE=TGAAAATGTTGTTGAATTTCTAGTCTTTTGAGTNAGGAAGGTAAACTAGAAGGGNGTAGAGTNGAGGGCTAGGGAGCCTGCCCACCATGGGCACCAAACTNTTATTGAAAGAAGGATGAAATCAGTATATTTGTGGCAGGCTGAAAGAGTCAGTGGGCTCAGGCCTCATGTATCAGCAGCAGGTGAAGAACTAGGAGCACCACTGGAAAAGAGTCATCTCCTACAACTGAATCTGGGCTCTCACTCTTTTTTTGCCCTTAACTATGCTGCAGCCCACAAAGGATCTGTGGAATGGGGGTATCTTGTTAGTCAACTTGTAT
SEQUENCE_INCLUDED_REGION=0,320
P3_COMMENT=CL is ATGTTGTTGAATTTCTAGTCTTTTG
P3_COMMENT=CR is ACAAGATACCCCCATTCCAC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.166033
PRIMER_LEFT_0_PENALTY=0.156061
PRIMER_RIGHT_0_PENALTY=0.009973
PRIMER_INTERNAL_0_PENALTY=1.043279
PRIMER_LEFT_0_SEQUENCE=GGAGCACCACTGGAAAAGAG
PRIMER_RIGHT_0_SEQUENCE=GCTGCAGCATAGTTAAGGGC
PRIMER_INTERNAL_0_SEQUENCE=CAACTGAATCTGGGCTCTCA
PRIMER_LEFT_0=193,20
PRIMER_RIGHT_0=272,20
PRIMER_INTERNAL_0=223,20
PRIMER_LEFT_0_TM=59.844
PRIMER_RIGHT_0_TM=60.010
PRIMER_INTERNAL_0_TM=58.957
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=10.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=0.166033
PRIMER_LEFT_1_PENALTY=0.156061
PRIMER_RIGHT_1_PENALTY=0.009973
PRIMER_INTERNAL_1_PENALTY=1.043279
PRIMER_LEFT_1_SEQUENCE=AGGAGCACCACTGGAAAAGA
PRIMER_RIGHT_1_SEQUENCE=GCTGCAGCATAGTTAAGGGC
PRIMER_INTERNAL_1_SEQUENCE=CAACTGAATCTGGGCTCTCA
PRIMER_LEFT_1=192,20
PRIMER_RIGHT_1=272,20
PRIMER_INTERNAL_1=223,20
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=60.010
PRIMER_INTERNAL_1_TM=58.957
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=81
PRIMER_PAIR_2_PENALTY=0.288917
PRIMER_LEFT_2_PENALTY=0.156061
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_INTERNAL_2_PENALTY=1.043279
PRIMER_LEFT_2_SEQUENCE=GGAGCACCACTGGAAAAGAG
PRIMER_RIGHT_2_SEQUENCE=GGCTGCAGCATAGTTAAGGG
PRIMER_INTERNAL_2_SEQUENCE=CAACTGAATCTGGGCTCTCA
PRIMER_LEFT_2=193,20
PRIMER_RIGHT_2=273,20
PRIMER_INTERNAL_2=223,20
PRIMER_LEFT_2_TM=59.844
PRIMER_RIGHT_2_TM=59.867
PRIMER_INTERNAL_2_TM=58.957
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=0.288917
PRIMER_LEFT_3_PENALTY=0.156061
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_INTERNAL_3_PENALTY=1.043279
PRIMER_LEFT_3_SEQUENCE=GGAGCACCACTGGAAAAGAG
PRIMER_RIGHT_3_SEQUENCE=GGGCTGCAGCATAGTTAAGG
PRIMER_INTERNAL_3_SEQUENCE=CAACTGAATCTGGGCTCTCA
PRIMER_LEFT_3=193,20
PRIMER_RIGHT_3=274,20
PRIMER_INTERNAL_3=223,20
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=59.867
PRIMER_INTERNAL_3_TM=58.957
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=0.288917
PRIMER_LEFT_4_PENALTY=0.156061
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_INTERNAL_4_PENALTY=1.043279
PRIMER_LEFT_4_SEQUENCE=AGGAGCACCACTGGAAAAGA
PRIMER_RIGHT_4_SEQUENCE=GGCTGCAGCATAGTTAAGGG
PRIMER_INTERNAL_4_SEQUENCE=CAACTGAATCTGGGCTCTCA
PRIMER_LEFT_4=192,20
PRIMER_RIGHT_4=273,20
PRIMER_INTERNAL_4=223,20
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=59.867
PRIMER_INTERNAL_4_TM=58.957
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH1327
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGAAATCTGTTCTCACCCCCACTCCTCCCCGCCTCATGGTCACCCTCCACTTTCTTTGGTCTGAAACCAATTGTGTAAATTAATTCACTTCTACATTGCTAATTGCTACGGAAGCTGAGAACTCAGAGGCTGCAAATACATCTTTTTGGGTTTTTTTGCCATTTTGAAAGTACAG
SEQUENCE_INCLUDED_REGION=35,178
P3_COMMENT=CL is AGAAATCTGTTCTCACCCCC
P3_COMMENT=CR is TTTGCAGCCTCTGAGTTCTC
P3_COMMENT=CM is CGCCTCATGGTCACCCTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.225404
PRIMER_LEFT_0_PENALTY=0.080729
PRIMER_RIGHT_0_PENALTY=0.144675
PRIMER_INTERNAL_0_PENALTY=1.786628
PRIMER_LEFT_0_SEQUENCE=TCACCCTCCACTTTCTTTGG
PRIMER_RIGHT_0_SEQUENCE=TTGCAGCCTCTGAGTTCTCA
PRIMER_INTERNAL_0_SEQUENCE=TTGCTAATTGCTACGGAAGC
PRIMER_LEFT_0=77,20
PRIMER_RIGHT_0=172,20
PRIMER_INTERNAL_0=133,20
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=59.855
PRIMER_INTERNAL_0_TM=58.213
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=0.520875
PRIMER_LEFT_1_PENALTY=0.080729
PRIMER_RIGHT_1_PENALTY=0.440146
PRIMER_INTERNAL_1_PENALTY=3.501838
PRIMER_LEFT_1_SEQUENCE=TCACCCTCCACTTTCTTTGG
PRIMER_RIGHT_1_SEQUENCE=GCAGCCTCTGAGTTCTCAGC
PRIMER_INTERNAL_1_SEQUENCE=ACATTGCTAATTGCTACGGAA
PRIMER_LEFT_1=77,20
PRIMER_RIGHT_1=170,20
PRIMER_INTERNAL_1=130,21
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.440
PRIMER_INTERNAL_1_TM=57.498
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=0.595518
PRIMER_LEFT_2_PENALTY=0.450843
PRIMER_RIGHT_2_PENALTY=0.144675
PRIMER_INTERNAL_2_PENALTY=1.786628
PRIMER_LEFT_2_SEQUENCE=TGGTCACCCTCCACTTTCTT
PRIMER_RIGHT_2_SEQUENCE=TTGCAGCCTCTGAGTTCTCA
PRIMER_INTERNAL_2_SEQUENCE=TTGCTAATTGCTACGGAAGC
PRIMER_LEFT_2=74,20
PRIMER_RIGHT_2=172,20
PRIMER_INTERNAL_2=133,20
PRIMER_LEFT_2_TM=59.549
PRIMER_RIGHT_2_TM=59.855
PRIMER_INTERNAL_2_TM=58.213
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=0.639975
PRIMER_LEFT_3_PENALTY=0.080729
PRIMER_RIGHT_3_PENALTY=0.559246
PRIMER_INTERNAL_3_PENALTY=3.357217
PRIMER_LEFT_3_SEQUENCE=TCACCCTCCACTTTCTTTGG
PRIMER_RIGHT_3_SEQUENCE=TGCAGCCTCTGAGTTCTCAG
PRIMER_INTERNAL_3_SEQUENCE=CATTGCTAATTGCTACGGAAG
PRIMER_LEFT_3=77,20
PRIMER_RIGHT_3=171,20
PRIMER_INTERNAL_3=131,21
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=59.441
PRIMER_INTERNAL_3_TM=57.643
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=8.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=0.803135
PRIMER_LEFT_4_PENALTY=0.362989
PRIMER_RIGHT_4_PENALTY=0.440146
PRIMER_INTERNAL_4_PENALTY=2.486265
PRIMER_LEFT_4_SEQUENCE=CATGGTCACCCTCCACTTTC
PRIMER_RIGHT_4_SEQUENCE=GCAGCCTCTGAGTTCTCAGC
PRIMER_INTERNAL_4_SEQUENCE=TTTGGTCTGAAACCAATTGTG
PRIMER_LEFT_4=72,20
PRIMER_RIGHT_4=170,20
PRIMER_INTERNAL_4=92,21
PRIMER_LEFT_4_TM=60.363
PRIMER_RIGHT_4_TM=60.440
PRIMER_INTERNAL_4_TM=58.514
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=9.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH1328
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTATGTTCACTATGGTTCTTCTACTCTGTCTTCTGTGTACGCTCATGTAGGGAAATGTTAGAATCAACATGTGACCTTTGAGATTGGTTAAGAAAGGTTGAGTCAGGGAGACATTTAG
SEQUENCE_INCLUDED_REGION=35,119
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.010555
PRIMER_LEFT_0_PENALTY=1.790576
PRIMER_RIGHT_0_PENALTY=2.219979
PRIMER_INTERNAL_0_PENALTY=3.022788
PRIMER_LEFT_0_SEQUENCE=TGTGTACGCTCATGTAGGGAA
PRIMER_RIGHT_0_SEQUENCE=TGTCTCCCTGACTCAACCTTT
PRIMER_INTERNAL_0_SEQUENCE=TCAACATGTGACCTTTGAGATTG
PRIMER_LEFT_0=69,21
PRIMER_RIGHT_0=148,21
PRIMER_INTERNAL_0=99,23
PRIMER_LEFT_0_TM=59.209
PRIMER_RIGHT_0_TM=58.780
PRIMER_INTERNAL_0_TM=60.023
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=4.237825
PRIMER_LEFT_1_PENALTY=2.017846
PRIMER_RIGHT_1_PENALTY=2.219979
PRIMER_INTERNAL_1_PENALTY=3.022788
PRIMER_LEFT_1_SEQUENCE=TGTACGCTCATGTAGGGAAATG
PRIMER_RIGHT_1_SEQUENCE=TGTCTCCCTGACTCAACCTTT
PRIMER_INTERNAL_1_SEQUENCE=TCAACATGTGACCTTTGAGATTG
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=148,21
PRIMER_INTERNAL_1=99,23
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=58.780
PRIMER_INTERNAL_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=4.406569
PRIMER_LEFT_2_PENALTY=2.186590
PRIMER_RIGHT_2_PENALTY=2.219979
PRIMER_INTERNAL_2_PENALTY=3.022788
PRIMER_LEFT_2_SEQUENCE=CTGTGTACGCTCATGTAGGGA
PRIMER_RIGHT_2_SEQUENCE=TGTCTCCCTGACTCAACCTTT
PRIMER_INTERNAL_2_SEQUENCE=TCAACATGTGACCTTTGAGATTG
PRIMER_LEFT_2=68,21
PRIMER_RIGHT_2=148,21
PRIMER_INTERNAL_2=99,23
PRIMER_LEFT_2_TM=58.813
PRIMER_RIGHT_2_TM=58.780
PRIMER_INTERNAL_2_TM=60.023
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=4.406569
PRIMER_LEFT_3_PENALTY=2.186590
PRIMER_RIGHT_3_PENALTY=2.219979
PRIMER_INTERNAL_3_PENALTY=3.022788
PRIMER_LEFT_3_SEQUENCE=TCTGTGTACGCTCATGTAGGG
PRIMER_RIGHT_3_SEQUENCE=TGTCTCCCTGACTCAACCTTT
PRIMER_INTERNAL_3_SEQUENCE=TCAACATGTGACCTTTGAGATTG
PRIMER_LEFT_3=67,21
PRIMER_RIGHT_3=148,21
PRIMER_INTERNAL_3=99,23
PRIMER_LEFT_3_TM=58.813
PRIMER_RIGHT_3_TM=58.780
PRIMER_INTERNAL_3_TM=60.023
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=4.409575
PRIMER_LEFT_4_PENALTY=2.189596
PRIMER_RIGHT_4_PENALTY=2.219979
PRIMER_INTERNAL_4_PENALTY=3.022788
PRIMER_LEFT_4_SEQUENCE=CTGTGTACGCTCATGTAGGGAA
PRIMER_RIGHT_4_SEQUENCE=TGTCTCCCTGACTCAACCTTT
PRIMER_INTERNAL_4_SEQUENCE=TCAACATGTGACCTTTGAGATTG
PRIMER_LEFT_4=68,22
PRIMER_RIGHT_4=148,21
PRIMER_INTERNAL_4=99,23
PRIMER_LEFT_4_TM=60.190
PRIMER_RIGHT_4_TM=58.780
PRIMER_INTERNAL_4_TM=60.023
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH1329
SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTTTGCTCCTGTCAGTCAGAGTNGCAGGTGCTTTTCTTTGCGTTCATGGAAATNATCCAATGGTATACACACAAGCGTCATCACTACAATTTTGGAAAAACCTGTGACTTGGAGGATTCGGGGATGTNTCATTGACTAGATGATTTGGGAGGCTCATCTGCACCCATTGGGCCTGTGCAGGTCTTGCAATAATCCTGTTGTCACCATCCAAGGACGTGAGGGCACCCTGCAAGG
SEQUENCE_INCLUDED_REGION=33,241
P3_COMMENT=CL is TTTTGCTCCTGTCAGTCAGA
P3_COMMENT=CR is CAACAGGATTATTGCAAGACC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.654688
PRIMER_LEFT_0_PENALTY=0.008354
PRIMER_RIGHT_0_PENALTY=0.646334
PRIMER_INTERNAL_0_PENALTY=2.003166
PRIMER_LEFT_0_SEQUENCE=TGCTTTTCTTTGCGTTCATG
PRIMER_RIGHT_0_SEQUENCE=CGAATCCTCCAAGTCACAGG
PRIMER_INTERNAL_0_SEQUENCE=GCGTCATCACTACAATTTTGGA
PRIMER_LEFT_0=68,20
PRIMER_RIGHT_0=159,20
PRIMER_INTERNAL_0=114,22
PRIMER_LEFT_0_TM=59.992
PRIMER_RIGHT_0_TM=60.646
PRIMER_INTERNAL_0_TM=60.003
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=0.654688
PRIMER_LEFT_1_PENALTY=0.008354
PRIMER_RIGHT_1_PENALTY=0.646334
PRIMER_INTERNAL_1_PENALTY=2.003166
PRIMER_LEFT_1_SEQUENCE=TGCTTTTCTTTGCGTTCATG
PRIMER_RIGHT_1_SEQUENCE=CCGAATCCTCCAAGTCACAG
PRIMER_INTERNAL_1_SEQUENCE=GCGTCATCACTACAATTTTGGA
PRIMER_LEFT_1=68,20
PRIMER_RIGHT_1=160,20
PRIMER_INTERNAL_1=114,22
PRIMER_LEFT_1_TM=59.992
PRIMER_RIGHT_1_TM=60.646
PRIMER_INTERNAL_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=0.782234
PRIMER_LEFT_2_PENALTY=0.646334
PRIMER_RIGHT_2_PENALTY=0.135900
PRIMER_INTERNAL_2_PENALTY=0.555419
PRIMER_LEFT_2_SEQUENCE=CTGTGACTTGGAGGATTCGG
PRIMER_RIGHT_2_SEQUENCE=TATTGCAAGACCTGCACAGG
PRIMER_INTERNAL_2_SEQUENCE=GATGATTTGGGAGGCTCATC
PRIMER_LEFT_2=141,20
PRIMER_RIGHT_2=230,20
PRIMER_INTERNAL_2=178,20
PRIMER_LEFT_2_TM=60.646
PRIMER_RIGHT_2_TM=59.864
PRIMER_INTERNAL_2_TM=59.445
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=0.782234
PRIMER_LEFT_3_PENALTY=0.646334
PRIMER_RIGHT_3_PENALTY=0.135900
PRIMER_INTERNAL_3_PENALTY=0.555419
PRIMER_LEFT_3_SEQUENCE=CCTGTGACTTGGAGGATTCG
PRIMER_RIGHT_3_SEQUENCE=TATTGCAAGACCTGCACAGG
PRIMER_INTERNAL_3_SEQUENCE=GATGATTTGGGAGGCTCATC
PRIMER_LEFT_3=140,20
PRIMER_RIGHT_3=230,20
PRIMER_INTERNAL_3=178,20
PRIMER_LEFT_3_TM=60.646
PRIMER_RIGHT_3_TM=59.864
PRIMER_INTERNAL_3_TM=59.445
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=1.014609
PRIMER_LEFT_4_PENALTY=0.008354
PRIMER_RIGHT_4_PENALTY=1.006255
PRIMER_INTERNAL_4_PENALTY=2.021762
PRIMER_LEFT_4_SEQUENCE=TGCTTTTCTTTGCGTTCATG
PRIMER_RIGHT_4_SEQUENCE=CCAAGTCACAGGTTTTTCCAA
PRIMER_INTERNAL_4_SEQUENCE=CCAATGGTATACACACAAGCG
PRIMER_LEFT_4=68,20
PRIMER_RIGHT_4=151,21
PRIMER_INTERNAL_4=96,21
PRIMER_LEFT_4_TM=59.992
PRIMER_RIGHT_4_TM=59.994
PRIMER_INTERNAL_4_TM=58.978
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH1337
SEQUENCE_TEMPLATE=TNNNTNCAGGTCGACTCTAGAGGATCCCCCTTCAGTGCTGAGGAAAGAAGTGGGAGCCGTATGGATACAGCAGGCAGAGAAGCACACATCATGTCCCAAAAGGCCAGGCATGACCAAGATCTGGCTGACGCTTCCAGAGAAAACGTGCAGACCCAGGATTTTNATGAGAAATCACTAGGTTTTTAAAAATGTTGACCAATCCCTCAAAGTAAATTTAAACCAATTGTGGATGAGTATTGTGAGGGCCAAATAAAGCCTGCCTGTGGGCCAGATGGCTGAATACCCAAAAGTCTGTGACCAGCCGGATAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC
SEQUENCE_INCLUDED_REGION=29,279
P3_COMMENT=CL is GAAAGAAGTGGGAGCCGTAT
P3_COMMENT=CR is TTGGCCCTCACAATACTCAT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.368740
PRIMER_LEFT_0_PENALTY=0.156061
PRIMER_RIGHT_0_PENALTY=0.212679
PRIMER_INTERNAL_0_PENALTY=0.032945
PRIMER_LEFT_0_SEQUENCE=GTGCTGAGGAAAGAAGTGGG
PRIMER_RIGHT_0_SEQUENCE=TCAGCCAGATCTTGGTCATG
PRIMER_INTERNAL_0_SEQUENCE=AGCACACATCATGTCCCAAA
PRIMER_LEFT_0=34,20
PRIMER_RIGHT_0=127,20
PRIMER_INTERNAL_0=80,20
PRIMER_LEFT_0_TM=59.844
PRIMER_RIGHT_0_TM=59.787
PRIMER_INTERNAL_0_TM=59.967
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=0.549303
PRIMER_LEFT_1_PENALTY=0.117817
PRIMER_RIGHT_1_PENALTY=0.431486
PRIMER_INTERNAL_1_PENALTY=0.032945
PRIMER_LEFT_1_SEQUENCE=AGCCGTATGGATACAGCAGG
PRIMER_RIGHT_1_SEQUENCE=CACGTTTTCTCTGGAAGCGT
PRIMER_INTERNAL_1_SEQUENCE=AGCACACATCATGTCCCAAA
PRIMER_LEFT_1=54,20
PRIMER_RIGHT_1=146,20
PRIMER_INTERNAL_1=80,20
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=60.431
PRIMER_INTERNAL_1_TM=59.967
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=0.551958
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=0.434141
PRIMER_INTERNAL_2_PENALTY=0.032945
PRIMER_LEFT_2_SEQUENCE=AGCCGTATGGATACAGCAGG
PRIMER_RIGHT_2_SEQUENCE=TCTGCACGTTTTCTCTGGAA
PRIMER_INTERNAL_2_SEQUENCE=AGCACACATCATGTCCCAAA
PRIMER_LEFT_2=54,20
PRIMER_RIGHT_2=150,20
PRIMER_INTERNAL_2=80,20
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=59.566
PRIMER_INTERNAL_2_TM=59.967
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=0.640260
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.522443
PRIMER_INTERNAL_3_PENALTY=0.032945
PRIMER_LEFT_3_SEQUENCE=AGCCGTATGGATACAGCAGG
PRIMER_RIGHT_3_SEQUENCE=ACGTTTTCTCTGGAAGCGTC
PRIMER_INTERNAL_3_SEQUENCE=AGCACACATCATGTCCCAAA
PRIMER_LEFT_3=54,20
PRIMER_RIGHT_3=145,20
PRIMER_INTERNAL_3=80,20
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=59.478
PRIMER_INTERNAL_3_TM=59.967
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=0.774911
PRIMER_LEFT_4_PENALTY=0.117817
PRIMER_RIGHT_4_PENALTY=0.657094
PRIMER_INTERNAL_4_PENALTY=0.032945
PRIMER_LEFT_4_SEQUENCE=AGCCGTATGGATACAGCAGG
PRIMER_RIGHT_4_SEQUENCE=TTTTCTCTGGAAGCGTCAGC
PRIMER_INTERNAL_4_SEQUENCE=AGCACACATCATGTCCCAAA
PRIMER_LEFT_4=54,20
PRIMER_RIGHT_4=142,20
PRIMER_INTERNAL_4=80,20
PRIMER_LEFT_4_TM=60.118
PRIMER_RIGHT_4_TM=60.657
PRIMER_INTERNAL_4_TM=59.967
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH139
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAAAAGTNGGAAGGCAGGACTGTGTTTTGGAGGACAAAAAGTAAAATCTTTTTATATCTTTATTTTTTAATTTTATTTTTTTTCAGGCATATAGACATACATATCCATTATACAACAGAAAAGGGGGCTGGAAAAGAAAGGTCAAGTGAGATTTCAGATATTCTTAAATGCAAGGCTGACAAATTTGGGCTTGATTCTGTAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA
SEQUENCE_INCLUDED_REGION=35,204
P3_COMMENT=CL is GAAGGCAGGACTGTGTTTTG
P3_COMMENT=CR is AATCAAGCCCAAATTTGTCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.090051
PRIMER_LEFT_0_PENALTY=0.103024
PRIMER_RIGHT_0_PENALTY=0.987026
PRIMER_INTERNAL_0_PENALTY=5.175845
PRIMER_LEFT_0_SEQUENCE=TACAACAGAAAAGGGGGCTG
PRIMER_RIGHT_0_SEQUENCE=AGCCCAAATTTGTCAGCCTT
PRIMER_INTERNAL_0_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
PRIMER_LEFT_0=149,20
PRIMER_RIGHT_0=229,20
PRIMER_INTERNAL_0=172,25
PRIMER_LEFT_0_TM=60.103
PRIMER_RIGHT_0_TM=60.987
PRIMER_INTERNAL_0_TM=59.824
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=36.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=1.090051
PRIMER_LEFT_1_PENALTY=0.103024
PRIMER_RIGHT_1_PENALTY=0.987026
PRIMER_INTERNAL_1_PENALTY=5.175845
PRIMER_LEFT_1_SEQUENCE=TACAACAGAAAAGGGGGCTG
PRIMER_RIGHT_1_SEQUENCE=AAGCCCAAATTTGTCAGCCT
PRIMER_INTERNAL_1_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
PRIMER_LEFT_1=149,20
PRIMER_RIGHT_1=230,20
PRIMER_INTERNAL_1=172,25
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=60.987
PRIMER_INTERNAL_1_TM=59.824
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=36.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=1.169167
PRIMER_LEFT_2_PENALTY=0.103024
PRIMER_RIGHT_2_PENALTY=1.066143
PRIMER_INTERNAL_2_PENALTY=5.175845
PRIMER_LEFT_2_SEQUENCE=TACAACAGAAAAGGGGGCTG
PRIMER_RIGHT_2_SEQUENCE=GCCCAAATTTGTCAGCCTT
PRIMER_INTERNAL_2_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
PRIMER_LEFT_2=149,20
PRIMER_RIGHT_2=228,19
PRIMER_INTERNAL_2=172,25
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=60.066
PRIMER_INTERNAL_2_TM=59.824
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.368
PRIMER_INTERNAL_2_GC_PERCENT=36.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=1.169167
PRIMER_LEFT_3_PENALTY=0.103024
PRIMER_RIGHT_3_PENALTY=1.066143
PRIMER_INTERNAL_3_PENALTY=5.175845
PRIMER_LEFT_3_SEQUENCE=TACAACAGAAAAGGGGGCTG
PRIMER_RIGHT_3_SEQUENCE=AAGCCCAAATTTGTCAGCC
PRIMER_INTERNAL_3_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA
PRIMER_LEFT_3=149,20
PRIMER_RIGHT_3=230,19
PRIMER_INTERNAL_3=172,25
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=60.066
PRIMER_INTERNAL_3_TM=59.824
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.368
PRIMER_INTERNAL_3_GC_PERCENT=36.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=1.172651
PRIMER_LEFT_4_PENALTY=0.103024
PRIMER_RIGHT_4_PENALTY=1.069627
PRIMER_INTERNAL_4_PENALTY=5.011655
PRIMER_LEFT_4_SEQUENCE=TACAACAGAAAAGGGGGCTG
PRIMER_RIGHT_4_SEQUENCE=GAATCAAGCCCAAATTTGTCA
PRIMER_INTERNAL_4_SEQUENCE=TTTCAGATATTCTTAAATGCAAGGC
PRIMER_LEFT_4=149,20
PRIMER_RIGHT_4=235,21
PRIMER_INTERNAL_4=190,25
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=59.930
PRIMER_INTERNAL_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH141
SEQUENCE_TEMPLATE=CCTGCAGGTCGACTCTAGAGGATCCCCCTGAATGCAAACATCACTGAGAAGTTTCTGAGAATGCTTCTGTTTGATTTTATATGAAGAAATTCCCGTTTCCAACGAAATCTTCAGAGGGGTACCGCGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAACTGCATTAATGAATCGGC
SEQUENCE_INCLUDED_REGION=27,89
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.132239
PRIMER_LEFT_0_PENALTY=2.678193
PRIMER_RIGHT_0_PENALTY=0.454047
PRIMER_INTERNAL_0_PENALTY=3.855762
PRIMER_LEFT_0_SEQUENCE=TGAATGCAAACATCACTGAGAA
PRIMER_RIGHT_0_SEQUENCE=GAAGATTTCGTTGGAAACGG
PRIMER_INTERNAL_0_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
PRIMER_LEFT_0=28,22
PRIMER_RIGHT_0=111,20
PRIMER_INTERNAL_0=51,23
PRIMER_LEFT_0_TM=59.322
PRIMER_RIGHT_0_TM=59.546
PRIMER_INTERNAL_0_TM=59.144
PRIMER_LEFT_0_GC_PERCENT=36.364
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=3.220455
PRIMER_LEFT_1_PENALTY=2.678193
PRIMER_RIGHT_1_PENALTY=0.542262
PRIMER_INTERNAL_1_PENALTY=3.855762
PRIMER_LEFT_1_SEQUENCE=TGAATGCAAACATCACTGAGAA
PRIMER_RIGHT_1_SEQUENCE=ATTTCGTTGGAAACGGGAAT
PRIMER_INTERNAL_1_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=107,20
PRIMER_INTERNAL_1=51,23
PRIMER_LEFT_1_TM=59.322
PRIMER_RIGHT_1_TM=60.542
PRIMER_INTERNAL_1_TM=59.144
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=3.528058
PRIMER_LEFT_2_PENALTY=3.074011
PRIMER_RIGHT_2_PENALTY=0.454047
PRIMER_INTERNAL_2_PENALTY=3.855762
PRIMER_LEFT_2_SEQUENCE=CTGAATGCAAACATCACTGAGA
PRIMER_RIGHT_2_SEQUENCE=GAAGATTTCGTTGGAAACGG
PRIMER_INTERNAL_2_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=111,20
PRIMER_INTERNAL_2=51,23
PRIMER_LEFT_2_TM=58.926
PRIMER_RIGHT_2_TM=59.546
PRIMER_INTERNAL_2_TM=59.144
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=3.616274
PRIMER_LEFT_3_PENALTY=3.074011
PRIMER_RIGHT_3_PENALTY=0.542262
PRIMER_INTERNAL_3_PENALTY=3.855762
PRIMER_LEFT_3_SEQUENCE=CTGAATGCAAACATCACTGAGA
PRIMER_RIGHT_3_SEQUENCE=ATTTCGTTGGAAACGGGAAT
PRIMER_INTERNAL_3_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=107,20
PRIMER_INTERNAL_3=51,23
PRIMER_LEFT_3_TM=58.926
PRIMER_RIGHT_3_TM=60.542
PRIMER_INTERNAL_3_TM=59.144
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=3.634131
PRIMER_LEFT_4_PENALTY=3.180084
PRIMER_RIGHT_4_PENALTY=0.454047
PRIMER_INTERNAL_4_PENALTY=3.855762
PRIMER_LEFT_4_SEQUENCE=TGAATGCAAACATCACTGAGA
PRIMER_RIGHT_4_SEQUENCE=GAAGATTTCGTTGGAAACGG
PRIMER_INTERNAL_4_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA
PRIMER_LEFT_4=28,21
PRIMER_RIGHT_4=111,20
PRIMER_INTERNAL_4=51,23
PRIMER_LEFT_4_TM=57.820
PRIMER_RIGHT_4_TM=59.546
PRIMER_INTERNAL_4_TM=59.144
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH142
SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCTGTAACAGTCTGGGTTGATTTGTTTTCCGATTTTGNTTTTTTTTAAATCACAGAAACATTTAGTTTCTTCATCTATACAATGGTGATAAAAATCTGTTACATGATCCTGACTTCAGGGCCATAGATAATTTGTCCTGAAGGGGATCCCTGGCCCGCTCTGTCCTTCAATATAGGTAGTAACTGCGGAACGTGAAGATCTAGAGCATGCTTTCCTCATGAAAATGCA
SEQUENCE_INCLUDED_REGION=34,227
P3_COMMENT=CL is TCTGGGTTGATTTGTTTTCC
P3_COMMENT=CR is TTTCATGAGGAAAGCATGCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.335696
PRIMER_LEFT_0_PENALTY=0.209870
PRIMER_RIGHT_0_PENALTY=0.125826
PRIMER_INTERNAL_0_PENALTY=0.132549
PRIMER_LEFT_0_SEQUENCE=CCTGACTTCAGGGCCATAGA
PRIMER_RIGHT_0_SEQUENCE=AGATCTTCACGTTCCGCAGT
PRIMER_INTERNAL_0_SEQUENCE=ATTTGTCCTGAAGGGGATCC
PRIMER_LEFT_0=140,20
PRIMER_RIGHT_0=233,20
PRIMER_INTERNAL_0=162,20
PRIMER_LEFT_0_TM=60.210
PRIMER_RIGHT_0_TM=59.874
PRIMER_INTERNAL_0_TM=60.133
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=0.335696
PRIMER_LEFT_1_PENALTY=0.209870
PRIMER_RIGHT_1_PENALTY=0.125826
PRIMER_INTERNAL_1_PENALTY=0.132549
PRIMER_LEFT_1_SEQUENCE=TCCTGACTTCAGGGCCATAG
PRIMER_RIGHT_1_SEQUENCE=AGATCTTCACGTTCCGCAGT
PRIMER_INTERNAL_1_SEQUENCE=ATTTGTCCTGAAGGGGATCC
PRIMER_LEFT_1=139,20
PRIMER_RIGHT_1=233,20
PRIMER_INTERNAL_1=162,20
PRIMER_LEFT_1_TM=60.210
PRIMER_RIGHT_1_TM=59.874
PRIMER_INTERNAL_1_TM=60.133
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=0.472724
PRIMER_LEFT_2_PENALTY=0.209870
PRIMER_RIGHT_2_PENALTY=0.262854
PRIMER_INTERNAL_2_PENALTY=0.132549
PRIMER_LEFT_2_SEQUENCE=CCTGACTTCAGGGCCATAGA
PRIMER_RIGHT_2_SEQUENCE=GATCTTCACGTTCCGCAGTT
PRIMER_INTERNAL_2_SEQUENCE=ATTTGTCCTGAAGGGGATCC
PRIMER_LEFT_2=140,20
PRIMER_RIGHT_2=232,20
PRIMER_INTERNAL_2=162,20
PRIMER_LEFT_2_TM=60.210
PRIMER_RIGHT_2_TM=60.263
PRIMER_INTERNAL_2_TM=60.133
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=93
PRIMER_PAIR_3_PENALTY=0.472724
PRIMER_LEFT_3_PENALTY=0.209870
PRIMER_RIGHT_3_PENALTY=0.262854
PRIMER_INTERNAL_3_PENALTY=0.132549
PRIMER_LEFT_3_SEQUENCE=TCCTGACTTCAGGGCCATAG
PRIMER_RIGHT_3_SEQUENCE=GATCTTCACGTTCCGCAGTT
PRIMER_INTERNAL_3_SEQUENCE=ATTTGTCCTGAAGGGGATCC
PRIMER_LEFT_3=139,20
PRIMER_RIGHT_3=232,20
PRIMER_INTERNAL_3=162,20
PRIMER_LEFT_3_TM=60.210
PRIMER_RIGHT_3_TM=60.263
PRIMER_INTERNAL_3_TM=60.133
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=0.618308
PRIMER_LEFT_4_PENALTY=0.492482
PRIMER_RIGHT_4_PENALTY=0.125826
PRIMER_INTERNAL_4_PENALTY=0.132549
PRIMER_LEFT_4_SEQUENCE=ATCCTGACTTCAGGGCCATA
PRIMER_RIGHT_4_SEQUENCE=AGATCTTCACGTTCCGCAGT
PRIMER_INTERNAL_4_SEQUENCE=ATTTGTCCTGAAGGGGATCC
PRIMER_LEFT_4=138,20
PRIMER_RIGHT_4=233,20
PRIMER_INTERNAL_4=162,20
PRIMER_LEFT_4_TM=59.508
PRIMER_RIGHT_4_TM=59.874
PRIMER_INTERNAL_4_TM=60.133
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH150
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTGATGCTTGTTTGTAACTGTCCAAAGGAAACCACTGCAACCTTCAAGCATGTCTGTGTTACTCTATTTTGTTCCAGCCACCTGTGGCATTTCCAAAATATGATAATCTCTGCCACCATACTGCTTTAAACACAAATAGAATCTGGCAGCAAAATATAGCATAAGCTTACTTCTAAATCAAAGGCTACCATCAGTACCTTAGCACATTTAAAAAATAAAAACCAACACTGCCCACTTCAATGAATGCTTTCAGCACACACCACAAAGGCTACTGGGG
SEQUENCE_INCLUDED_REGION=35,283
P3_COMMENT=CL is AAACCACTGCAACCTTCAAG
P3_COMMENT=CR is TGGGCAGTGTTGGTTTTTAT
P3_COMMENT=CM is GTGTTACTCTATTTTGTTCCAGCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.082828
PRIMER_LEFT_0_PENALTY=0.536418
PRIMER_RIGHT_0_PENALTY=0.546410
PRIMER_INTERNAL_0_PENALTY=0.298898
PRIMER_LEFT_0_SEQUENCE=CCTGCTTTGATGCTTGTTTG
PRIMER_RIGHT_0_SEQUENCE=ACAGGTGGCTGGAACAAAAT
PRIMER_INTERNAL_0_SEQUENCE=AACCACTGCAACCTTCAAGC
PRIMER_LEFT_0=35,20
PRIMER_RIGHT_0=125,20
PRIMER_INTERNAL_0=70,20
PRIMER_LEFT_0_TM=59.464
PRIMER_RIGHT_0_TM=59.454
PRIMER_INTERNAL_0_TM=60.299
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=1.236370
PRIMER_LEFT_1_PENALTY=0.528861
PRIMER_RIGHT_1_PENALTY=0.707509
PRIMER_INTERNAL_1_PENALTY=5.467656
PRIMER_LEFT_1_SEQUENCE=CAAAGGAAACCACTGCAACC
PRIMER_RIGHT_1_SEQUENCE=ATTTTGGAAATGCCACAGGT
PRIMER_INTERNAL_1_SEQUENCE=TGTTACTCTATTTTGTTCCAGCC
PRIMER_LEFT_1=63,20
PRIMER_RIGHT_1=139,20
PRIMER_INTERNAL_1=97,23
PRIMER_LEFT_1_TM=60.529
PRIMER_RIGHT_1_TM=59.292
PRIMER_INTERNAL_1_TM=57.532
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=1.236370
PRIMER_LEFT_2_PENALTY=0.528861
PRIMER_RIGHT_2_PENALTY=0.707509
PRIMER_INTERNAL_2_PENALTY=5.467656
PRIMER_LEFT_2_SEQUENCE=CCAAAGGAAACCACTGCAAC
PRIMER_RIGHT_2_SEQUENCE=ATTTTGGAAATGCCACAGGT
PRIMER_INTERNAL_2_SEQUENCE=TGTTACTCTATTTTGTTCCAGCC
PRIMER_LEFT_2=62,20
PRIMER_RIGHT_2=139,20
PRIMER_INTERNAL_2=97,23
PRIMER_LEFT_2_TM=60.529
PRIMER_RIGHT_2_TM=59.292
PRIMER_INTERNAL_2_TM=57.532
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=1.408997
PRIMER_LEFT_3_PENALTY=0.298898
PRIMER_RIGHT_3_PENALTY=1.110099
PRIMER_INTERNAL_3_PENALTY=0.546410
PRIMER_LEFT_3_SEQUENCE=AACCACTGCAACCTTCAAGC
PRIMER_RIGHT_3_SEQUENCE=AAAGCAGTATGGTGGCAGAGA
PRIMER_INTERNAL_3_SEQUENCE=ATTTTGTTCCAGCCACCTGT
PRIMER_LEFT_3=70,20
PRIMER_RIGHT_3=167,21
PRIMER_INTERNAL_3=106,20
PRIMER_LEFT_3_TM=60.299
PRIMER_RIGHT_3_TM=59.890
PRIMER_INTERNAL_3_TM=59.454
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=1.559540
PRIMER_LEFT_4_PENALTY=0.449441
PRIMER_RIGHT_4_PENALTY=1.110099
PRIMER_INTERNAL_4_PENALTY=0.546410
PRIMER_LEFT_4_SEQUENCE=CACTGCAACCTTCAAGCATG
PRIMER_RIGHT_4_SEQUENCE=AAAGCAGTATGGTGGCAGAGA
PRIMER_INTERNAL_4_SEQUENCE=ATTTTGTTCCAGCCACCTGT
PRIMER_LEFT_4=73,20
PRIMER_RIGHT_4=167,21
PRIMER_INTERNAL_4=106,20
PRIMER_LEFT_4_TM=60.449
PRIMER_RIGHT_4_TM=59.890
PRIMER_INTERNAL_4_TM=59.454
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH152
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATAATTATAAGACACCTTTATGCCCCAGGACATCCAGGTTCTTTACAGACTTTAGCCCTCTCAGGAGGCTGGTGGCATTTTGCCATTTTACAGATGAAGGTGATTATAAACAAAGCATTGAAAAGATGTATTGCACAGCAGGTTGCAGGAATTTGTGCCAGTATAAAACACCTTCTTTGTTCGCTACTAGTGTTAACATCTATAATGTGCTTAGATTGTTTTTCTCATCCTTAATACAGGGTACCGAG
SEQUENCE_INCLUDED_REGION=35,252
P3_COMMENT=CL is TAAGACACCTTTATGCCCCA
P3_COMMENT=CR is AAGGATGAGAAAAACAATCTAAGC
P3_COMMENT=CM is GACATCCAGGTTCTTTACAGACTT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.321121
PRIMER_LEFT_0_PENALTY=0.064105
PRIMER_RIGHT_0_PENALTY=0.257016
PRIMER_INTERNAL_0_PENALTY=2.627348
PRIMER_LEFT_0_SEQUENCE=TGGCATTTTGCCATTTTACA
PRIMER_RIGHT_0_SEQUENCE=TACTGGCACAAATTCCTGCA
PRIMER_INTERNAL_0_SEQUENCE=TGAAAAGATGTATTGCACAGCA
PRIMER_LEFT_0=111,20
PRIMER_RIGHT_0=201,20
PRIMER_INTERNAL_0=157,22
PRIMER_LEFT_0_TM=59.936
PRIMER_RIGHT_0_TM=60.257
PRIMER_INTERNAL_0_TM=59.373
PRIMER_LEFT_0_GC_PERCENT=35.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=0.323809
PRIMER_LEFT_1_PENALTY=0.064105
PRIMER_RIGHT_1_PENALTY=0.259704
PRIMER_INTERNAL_1_PENALTY=4.031619
PRIMER_LEFT_1_SEQUENCE=TGGCATTTTGCCATTTTACA
PRIMER_RIGHT_1_SEQUENCE=AAATTCCTGCAACCTGCTGT
PRIMER_INTERNAL_1_SEQUENCE=CAAAGCATTGAAAAGATGTATTGC
PRIMER_LEFT_1=111,20
PRIMER_RIGHT_1=192,20
PRIMER_INTERNAL_1=149,24
PRIMER_LEFT_1_TM=59.936
PRIMER_RIGHT_1_TM=59.740
PRIMER_INTERNAL_1_TM=60.032
PRIMER_LEFT_1_GC_PERCENT=35.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.323809
PRIMER_LEFT_2_PENALTY=0.064105
PRIMER_RIGHT_2_PENALTY=0.259704
PRIMER_INTERNAL_2_PENALTY=4.031619
PRIMER_LEFT_2_SEQUENCE=TGGCATTTTGCCATTTTACA
PRIMER_RIGHT_2_SEQUENCE=ACAAATTCCTGCAACCTGCT
PRIMER_INTERNAL_2_SEQUENCE=AAAGCATTGAAAAGATGTATTGCA
PRIMER_LEFT_2=111,20
PRIMER_RIGHT_2=194,20
PRIMER_INTERNAL_2=150,24
PRIMER_LEFT_2_TM=59.936
PRIMER_RIGHT_2_TM=59.740
PRIMER_INTERNAL_2_TM=60.032
PRIMER_LEFT_2_GC_PERCENT=35.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=84
PRIMER_PAIR_3_PENALTY=0.642473
PRIMER_LEFT_3_PENALTY=0.578368
PRIMER_RIGHT_3_PENALTY=0.064105
PRIMER_INTERNAL_3_PENALTY=0.475706
PRIMER_LEFT_3_SEQUENCE=ACCTTTATGCCCCAGGACAT
PRIMER_RIGHT_3_SEQUENCE=TGTAAAATGGCAAAATGCCA
PRIMER_INTERNAL_3_SEQUENCE=TTTAGCCCTCTCAGGAGGCT
PRIMER_LEFT_3=52,20
PRIMER_RIGHT_3=130,20
PRIMER_INTERNAL_3=89,20
PRIMER_LEFT_3_TM=60.578
PRIMER_RIGHT_3_TM=59.936
PRIMER_INTERNAL_3_TM=60.476
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=35.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=7.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=0.937502
PRIMER_LEFT_4_PENALTY=0.680486
PRIMER_RIGHT_4_PENALTY=0.257016
PRIMER_INTERNAL_4_PENALTY=2.627348
PRIMER_LEFT_4_SEQUENCE=GGTGGCATTTTGCCATTTTA
PRIMER_RIGHT_4_SEQUENCE=TACTGGCACAAATTCCTGCA
PRIMER_INTERNAL_4_SEQUENCE=TGAAAAGATGTATTGCACAGCA
PRIMER_LEFT_4=109,20
PRIMER_RIGHT_4=201,20
PRIMER_INTERNAL_4=157,22
PRIMER_LEFT_4_TM=60.680
PRIMER_RIGHT_4_TM=60.257
PRIMER_INTERNAL_4_TM=59.373
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=36.364
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH154
SEQUENCE_TEMPLATE=GTTTTGATGCTGCAGGTCGACTCTAGAGGATCCCCTAAGAAAACAGTGTNGTAATTCCCTGGCTTTGCACATGTTCCTTCATCTCATCATCTGCCCTAAAGTNGATGCTGCTCCAATATTCAATGTGGATTTATACAAACCACTGCATGACACAGCAAACATGGTTGGACCTCATTCATATACCCCTGGCTCACCATGCAAATCACCTGCAGACAGTTCTCACACATGGCCAAGGCTTTTCTGTGTCTGTGGGCAGGCTTGGGCCATGAAGTGTGCATGTGGCCAGATCAGAGAGTTAATTCCCCAGGGAGTGACCTTCTACTACCGTGAAGGGCAGGATGTGGTAGATAAGTAGTTCAGTTTCCTCA
SEQUENCE_INCLUDED_REGION=34,334
P3_COMMENT=CL is GTAATTCCCTGGCTTTGCAC
P3_COMMENT=CR is TTATCTACCACATCCTGCCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.170673
PRIMER_LEFT_0_PENALTY=0.008216
PRIMER_RIGHT_0_PENALTY=0.162457
PRIMER_INTERNAL_0_PENALTY=0.112124
PRIMER_LEFT_0_SEQUENCE=CACAGCAAACATGGTTGGAC
PRIMER_RIGHT_0_SEQUENCE=TGGCCATGTGTGAGAACTGT
PRIMER_INTERNAL_0_SEQUENCE=CTCACCATGCAAATCACCTG
PRIMER_LEFT_0=150,20
PRIMER_RIGHT_0=231,20
PRIMER_INTERNAL_0=189,20
PRIMER_LEFT_0_TM=60.008
PRIMER_RIGHT_0_TM=60.162
PRIMER_INTERNAL_0_TM=60.112
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=0.170673
PRIMER_LEFT_1_PENALTY=0.008216
PRIMER_RIGHT_1_PENALTY=0.162457
PRIMER_INTERNAL_1_PENALTY=0.112124
PRIMER_LEFT_1_SEQUENCE=ACACAGCAAACATGGTTGGA
PRIMER_RIGHT_1_SEQUENCE=TGGCCATGTGTGAGAACTGT
PRIMER_INTERNAL_1_SEQUENCE=CTCACCATGCAAATCACCTG
PRIMER_LEFT_1=149,20
PRIMER_RIGHT_1=231,20
PRIMER_INTERNAL_1=189,20
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.162
PRIMER_INTERNAL_1_TM=60.112
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.170673
PRIMER_LEFT_2_PENALTY=0.008216
PRIMER_RIGHT_2_PENALTY=0.162457
PRIMER_INTERNAL_2_PENALTY=0.112124
PRIMER_LEFT_2_SEQUENCE=GACACAGCAAACATGGTTGG
PRIMER_RIGHT_2_SEQUENCE=TGGCCATGTGTGAGAACTGT
PRIMER_INTERNAL_2_SEQUENCE=CTCACCATGCAAATCACCTG
PRIMER_LEFT_2=148,20
PRIMER_RIGHT_2=231,20
PRIMER_INTERNAL_2=189,20
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.162
PRIMER_INTERNAL_2_TM=60.112
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=84
PRIMER_PAIR_3_PENALTY=0.188989
PRIMER_LEFT_3_PENALTY=0.026532
PRIMER_RIGHT_3_PENALTY=0.162457
PRIMER_INTERNAL_3_PENALTY=0.112124
PRIMER_LEFT_3_SEQUENCE=AGCAAACATGGTTGGACCTC
PRIMER_RIGHT_3_SEQUENCE=TGGCCATGTGTGAGAACTGT
PRIMER_INTERNAL_3_SEQUENCE=CTCACCATGCAAATCACCTG
PRIMER_LEFT_3=153,20
PRIMER_RIGHT_3=231,20
PRIMER_INTERNAL_3=189,20
PRIMER_LEFT_3_TM=59.973
PRIMER_RIGHT_3_TM=60.162
PRIMER_INTERNAL_3_TM=60.112
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=0.353715
PRIMER_LEFT_4_PENALTY=0.226618
PRIMER_RIGHT_4_PENALTY=0.127097
PRIMER_INTERNAL_4_PENALTY=0.162457
PRIMER_LEFT_4_SEQUENCE=TCATATACCCCTGGCTCACC
PRIMER_RIGHT_4_SEQUENCE=TGCCCACAGACACAGAAAAG
PRIMER_INTERNAL_4_SEQUENCE=ACAGTTCTCACACATGGCCA
PRIMER_LEFT_4=175,20
PRIMER_RIGHT_4=254,20
PRIMER_INTERNAL_4=212,20
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.873
PRIMER_INTERNAL_4_TM=60.162
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH157
SEQUENCE_TEMPLATE=AGATTGNCTGCCTGCNGGTCGACTCTAGAGGATCCCCCTCTATTGATACGTGCCTACTTATTCACTTTGGTTTGTGTTTTATGTTGCTGGTATCATCATCTCTGTTTGACTCTTCTCATCTGTCTTGCCCACTGTTCCTAGTTGTCTCTCTCTTTTCTTTCCAGACTTCTAGTTAAGTATCTTATTCTAAGCATCAGATGGTAAAT
SEQUENCE_INCLUDED_REGION=36,170
P3_COMMENT=CL is TACGTGCCTACTTATTCACTTTG
P3_COMMENT=CR is AGAGACAACTAGGAACAGTGGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.466107
PRIMER_LEFT_0_PENALTY=3.168089
PRIMER_RIGHT_0_PENALTY=0.298018
PRIMER_INTERNAL_0_PENALTY=3.146910
PRIMER_LEFT_0_SEQUENCE=CGTGCCTACTTATTCACTTTGG
PRIMER_RIGHT_0_SEQUENCE=AGGAACAGTGGGCAAGACAG
PRIMER_INTERNAL_0_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
PRIMER_LEFT_0=48,22
PRIMER_RIGHT_0=138,20
PRIMER_INTERNAL_0=81,23
PRIMER_LEFT_0_TM=58.832
PRIMER_RIGHT_0_TM=60.298
PRIMER_INTERNAL_0_TM=60.147
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=3.687734
PRIMER_LEFT_1_PENALTY=3.389716
PRIMER_RIGHT_1_PENALTY=0.298018
PRIMER_INTERNAL_1_PENALTY=3.146910
PRIMER_LEFT_1_SEQUENCE=CGTGCCTACTTATTCACTTTGGT
PRIMER_RIGHT_1_SEQUENCE=AGGAACAGTGGGCAAGACAG
PRIMER_INTERNAL_1_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
PRIMER_LEFT_1=48,23
PRIMER_RIGHT_1=138,20
PRIMER_INTERNAL_1=81,23
PRIMER_LEFT_1_TM=59.610
PRIMER_RIGHT_1_TM=60.298
PRIMER_INTERNAL_1_TM=60.147
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=3.687734
PRIMER_LEFT_2_PENALTY=3.389716
PRIMER_RIGHT_2_PENALTY=0.298018
PRIMER_INTERNAL_2_PENALTY=3.146910
PRIMER_LEFT_2_SEQUENCE=ACGTGCCTACTTATTCACTTTGG
PRIMER_RIGHT_2_SEQUENCE=AGGAACAGTGGGCAAGACAG
PRIMER_INTERNAL_2_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
PRIMER_LEFT_2=47,23
PRIMER_RIGHT_2=138,20
PRIMER_INTERNAL_2=81,23
PRIMER_LEFT_2_TM=59.610
PRIMER_RIGHT_2_TM=60.298
PRIMER_INTERNAL_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=3.739324
PRIMER_LEFT_3_PENALTY=2.575721
PRIMER_RIGHT_3_PENALTY=1.163603
PRIMER_INTERNAL_3_PENALTY=5.064245
PRIMER_LEFT_3_SEQUENCE=GGTTTGTGTTTTATGTTGCTGG
PRIMER_RIGHT_3_SEQUENCE=GACAACTAGGAACAGTGGGCA
PRIMER_INTERNAL_3_SEQUENCE=CATCATCTCTGTTTGACTCTTCTCA
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=145,21
PRIMER_INTERNAL_3=93,25
PRIMER_LEFT_3_TM=59.424
PRIMER_RIGHT_3_TM=60.164
PRIMER_INTERNAL_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=3.753478
PRIMER_LEFT_4_PENALTY=3.168089
PRIMER_RIGHT_4_PENALTY=0.585389
PRIMER_INTERNAL_4_PENALTY=3.146910
PRIMER_LEFT_4_SEQUENCE=CGTGCCTACTTATTCACTTTGG
PRIMER_RIGHT_4_SEQUENCE=CAGTGGGCAAGACAGATGAG
PRIMER_INTERNAL_4_SEQUENCE=TGTTGCTGGTATCATCATCTCTG
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=133,20
PRIMER_INTERNAL_4=81,23
PRIMER_LEFT_4_TM=58.832
PRIMER_RIGHT_4_TM=59.415
PRIMER_INTERNAL_4_TM=60.147
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH159
SEQUENCE_TEMPLATE=CTCCAGGCCAACAGAGCCCCAGATGTGGAAGTAGAGTNNGTTGGACTGCAGTCCCACTGGCTACAATCAAACATGGCAGGCCAGATCTGTTCAGTATTAGTNCTGGNTCCAAAACCTATACATCTAGATGTNTTTCAGAATTCAGAATTATTTAGATTTTGGAAAGGTAATTTGGCATGTATGTGTGTATATTTAATAATACACTGATGGAGTCAGGGGATAGTATGCCATAATCGAACATTATTATCTTCATAGCAAATGTATGA
SEQUENCE_INCLUDED_REGION=0,266
P3_COMMENT=CL is CCCACTGGCTACAATCAAAC
P3_COMMENT=CR is TTTGCTATGAAGATAATAATGTTCG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.490504
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=0.417552
PRIMER_INTERNAL_0_PENALTY=0.141511
PRIMER_LEFT_0_SEQUENCE=GCCCCAGATGTGGAAGTAGA
PRIMER_RIGHT_0_SEQUENCE=ACTGAACAGATCTGGCCTGC
PRIMER_INTERNAL_0_SEQUENCE=GCAGTCCCACTGGCTACAAT
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=94,20
PRIMER_INTERNAL_0=47,20
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=60.418
PRIMER_INTERNAL_0_TM=60.142
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=0.490504
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.417552
PRIMER_INTERNAL_1_PENALTY=0.141511
PRIMER_LEFT_1_SEQUENCE=GAGCCCCAGATGTGGAAGTA
PRIMER_RIGHT_1_SEQUENCE=ACTGAACAGATCTGGCCTGC
PRIMER_INTERNAL_1_SEQUENCE=GCAGTCCCACTGGCTACAAT
PRIMER_LEFT_1=13,20
PRIMER_RIGHT_1=94,20
PRIMER_INTERNAL_1=47,20
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=60.418
PRIMER_INTERNAL_1_TM=60.142
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=1.255488
PRIMER_LEFT_2_PENALTY=0.837936
PRIMER_RIGHT_2_PENALTY=0.417552
PRIMER_INTERNAL_2_PENALTY=0.141511
PRIMER_LEFT_2_SEQUENCE=AGCCCCAGATGTGGAAGTAG
PRIMER_RIGHT_2_SEQUENCE=ACTGAACAGATCTGGCCTGC
PRIMER_INTERNAL_2_SEQUENCE=GCAGTCCCACTGGCTACAAT
PRIMER_LEFT_2=14,20
PRIMER_RIGHT_2=94,20
PRIMER_INTERNAL_2=47,20
PRIMER_LEFT_2_TM=59.162
PRIMER_RIGHT_2_TM=60.418
PRIMER_INTERNAL_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=1.469898
PRIMER_LEFT_3_PENALTY=1.052345
PRIMER_RIGHT_3_PENALTY=0.417552
PRIMER_INTERNAL_3_PENALTY=0.141511
PRIMER_LEFT_3_SEQUENCE=AGAGCCCCAGATGTGGAAGT
PRIMER_RIGHT_3_SEQUENCE=ACTGAACAGATCTGGCCTGC
PRIMER_INTERNAL_3_SEQUENCE=GCAGTCCCACTGGCTACAAT
PRIMER_LEFT_3=12,20
PRIMER_RIGHT_3=94,20
PRIMER_INTERNAL_3=47,20
PRIMER_LEFT_3_TM=61.052
PRIMER_RIGHT_3_TM=60.418
PRIMER_INTERNAL_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=1.481742
PRIMER_LEFT_4_PENALTY=1.064190
PRIMER_RIGHT_4_PENALTY=0.417552
PRIMER_INTERNAL_4_PENALTY=0.141511
PRIMER_LEFT_4_SEQUENCE=GAGCCCCAGATGTGGAAGT
PRIMER_RIGHT_4_SEQUENCE=ACTGAACAGATCTGGCCTGC
PRIMER_INTERNAL_4_SEQUENCE=GCAGTCCCACTGGCTACAAT
PRIMER_LEFT_4=13,19
PRIMER_RIGHT_4=94,20
PRIMER_INTERNAL_4=47,20
PRIMER_LEFT_4_TM=60.064
PRIMER_RIGHT_4_TM=60.418
PRIMER_INTERNAL_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH160
SEQUENCE_TEMPLATE=CTGTGGGAAAAATAAGGCCTTGAAGAATGAAAAAAGTTCTATTAAAACAATTCTATTTAGGATCTGGTTACACACATTCATAAAACTAAAGATGTNNGATTCTGATTNGATTGGTAAAGTCAGTTAGNCACAAAGTAAAGAATCAAACTTAAAGGNTACAGGNCAGAGACTGAAGNTCATTGTTAAGANGGCAATGAACACCTTTAGTCTAATAAAG
SEQUENCE_INCLUDED_REGION=0,217
P3_COMMENT=CL is TGGGAAAAATAAGGCCTTGA
P3_COMMENT=CR is TATTAGACTAAAGGTGTTCATTGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=10.419566
PRIMER_LEFT_0_PENALTY=4.951910
PRIMER_RIGHT_0_PENALTY=5.467656
PRIMER_INTERNAL_0_PENALTY=8.811170
PRIMER_LEFT_0_SEQUENCE=AGGATCTGGTTACACACATTCA
PRIMER_RIGHT_0_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
PRIMER_INTERNAL_0_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
PRIMER_LEFT_0=58,22
PRIMER_RIGHT_0=211,23
PRIMER_INTERNAL_0=128,27
PRIMER_LEFT_0_TM=57.048
PRIMER_RIGHT_0_TM=57.532
PRIMER_INTERNAL_0_TM=58.189
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_INTERNAL_0_GC_PERCENT=29.630
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=154
PRIMER_PAIR_1_PENALTY=10.646861
PRIMER_LEFT_1_PENALTY=5.179204
PRIMER_RIGHT_1_PENALTY=5.467656
PRIMER_INTERNAL_1_PENALTY=8.811170
PRIMER_LEFT_1_SEQUENCE=TTTAGGATCTGGTTACACACATTCA
PRIMER_RIGHT_1_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
PRIMER_INTERNAL_1_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
PRIMER_LEFT_1=55,25
PRIMER_RIGHT_1=211,23
PRIMER_INTERNAL_1=128,27
PRIMER_LEFT_1_TM=59.821
PRIMER_RIGHT_1_TM=57.532
PRIMER_INTERNAL_1_TM=58.189
PRIMER_LEFT_1_GC_PERCENT=36.000
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_INTERNAL_1_GC_PERCENT=29.630
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=157
PRIMER_PAIR_2_PENALTY=10.904149
PRIMER_LEFT_2_PENALTY=5.436493
PRIMER_RIGHT_2_PENALTY=5.467656
PRIMER_INTERNAL_2_PENALTY=8.811170
PRIMER_LEFT_2_SEQUENCE=TTAGGATCTGGTTACACACATTCA
PRIMER_RIGHT_2_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
PRIMER_INTERNAL_2_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
PRIMER_LEFT_2=56,24
PRIMER_RIGHT_2=211,23
PRIMER_INTERNAL_2=128,27
PRIMER_LEFT_2_TM=58.564
PRIMER_RIGHT_2_TM=57.532
PRIMER_INTERNAL_2_TM=58.189
PRIMER_LEFT_2_GC_PERCENT=37.500
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_INTERNAL_2_GC_PERCENT=29.630
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=156
PRIMER_PAIR_3_PENALTY=10.995102
PRIMER_LEFT_3_PENALTY=5.527445
PRIMER_RIGHT_3_PENALTY=5.467656
PRIMER_INTERNAL_3_PENALTY=8.811170
PRIMER_LEFT_3_SEQUENCE=AGGATCTGGTTACACACATTCAT
PRIMER_RIGHT_3_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC
PRIMER_INTERNAL_3_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
PRIMER_LEFT_3=58,23
PRIMER_RIGHT_3=211,23
PRIMER_INTERNAL_3=128,27
PRIMER_LEFT_3_TM=57.473
PRIMER_RIGHT_3_TM=57.532
PRIMER_INTERNAL_3_TM=58.189
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_INTERNAL_3_GC_PERCENT=29.630
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=154
PRIMER_PAIR_4_PENALTY=11.064529
PRIMER_LEFT_4_PENALTY=4.951910
PRIMER_RIGHT_4_PENALTY=6.112620
PRIMER_INTERNAL_4_PENALTY=8.811170
PRIMER_LEFT_4_SEQUENCE=AGGATCTGGTTACACACATTCA
PRIMER_RIGHT_4_SEQUENCE=ATTAGACTAAAGGTGTTCATTGCC
PRIMER_INTERNAL_4_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG
PRIMER_LEFT_4=58,22
PRIMER_RIGHT_4=212,24
PRIMER_INTERNAL_4=128,27
PRIMER_LEFT_4_TM=57.048
PRIMER_RIGHT_4_TM=57.887
PRIMER_INTERNAL_4_TM=58.189
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=37.500
PRIMER_INTERNAL_4_GC_PERCENT=29.630
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=155
=
SEQUENCE_ID=MH161
SEQUENCE_TEMPLATE=CTCTTACTAATAGGATGGGAGAGTNNGAAGGAACATCAGAGATTATTTTACAGATAACAAAACTAAGACTCAGATTAAGATGCTTCTTCATGGTTACTGTAGGATTTTAGGCACCTCACTCTTTCCAAATTGCTCTAATTGACAATTCAAACAAAATTCTACCCTCTTGTGCCTCATCCATTCATACATCCTTTCTGTCCACAACAGTGTACTTTATACATGTATATACACCCACCTACATACATATAG
SEQUENCE_INCLUDED_REGION=0,249
P3_COMMENT=CL is CTCTTACTAATAGGATGGGAGAGT
P3_COMMENT=CR is TACACTGTTGTGGACAGAAAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.357046
PRIMER_LEFT_0_PENALTY=0.156061
PRIMER_RIGHT_0_PENALTY=0.200985
PRIMER_INTERNAL_0_PENALTY=5.051029
PRIMER_LEFT_0_SEQUENCE=AGGCACCTCACTCTTTCCAA
PRIMER_RIGHT_0_SEQUENCE=TGAATGGATGAGGCACAAGA
PRIMER_INTERNAL_0_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
PRIMER_LEFT_0=108,20
PRIMER_RIGHT_0=183,20
PRIMER_INTERNAL_0=130,25
PRIMER_LEFT_0_TM=59.844
PRIMER_RIGHT_0_TM=60.201
PRIMER_INTERNAL_0_TM=60.051
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=28.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=9.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=0.433284
PRIMER_LEFT_1_PENALTY=0.232299
PRIMER_RIGHT_1_PENALTY=0.200985
PRIMER_INTERNAL_1_PENALTY=5.051029
PRIMER_LEFT_1_SEQUENCE=GGCACCTCACTCTTTCCAAA
PRIMER_RIGHT_1_SEQUENCE=TGAATGGATGAGGCACAAGA
PRIMER_INTERNAL_1_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
PRIMER_LEFT_1=109,20
PRIMER_RIGHT_1=183,20
PRIMER_INTERNAL_1=130,25
PRIMER_LEFT_1_TM=60.232
PRIMER_RIGHT_1_TM=60.201
PRIMER_INTERNAL_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=28.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=9.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=1.212143
PRIMER_LEFT_2_PENALTY=1.139192
PRIMER_RIGHT_2_PENALTY=0.072951
PRIMER_INTERNAL_2_PENALTY=5.051029
PRIMER_LEFT_2_SEQUENCE=TAGGCACCTCACTCTTTCCAA
PRIMER_RIGHT_2_SEQUENCE=AATGGATGAGGCACAAGAGG
PRIMER_INTERNAL_2_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
PRIMER_LEFT_2=107,21
PRIMER_RIGHT_2=181,20
PRIMER_INTERNAL_2=130,25
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=60.073
PRIMER_INTERNAL_2_TM=60.051
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=28.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=9.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=1.212143
PRIMER_LEFT_3_PENALTY=1.139192
PRIMER_RIGHT_3_PENALTY=0.072951
PRIMER_INTERNAL_3_PENALTY=5.051029
PRIMER_LEFT_3_SEQUENCE=TTAGGCACCTCACTCTTTCCA
PRIMER_RIGHT_3_SEQUENCE=AATGGATGAGGCACAAGAGG
PRIMER_INTERNAL_3_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
PRIMER_LEFT_3=106,21
PRIMER_RIGHT_3=181,20
PRIMER_INTERNAL_3=130,25
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=60.073
PRIMER_INTERNAL_3_TM=60.051
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=28.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=9.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=1.216344
PRIMER_LEFT_4_PENALTY=0.156061
PRIMER_RIGHT_4_PENALTY=1.060283
PRIMER_INTERNAL_4_PENALTY=5.051029
PRIMER_LEFT_4_SEQUENCE=AGGCACCTCACTCTTTCCAA
PRIMER_RIGHT_4_SEQUENCE=TGTATGAATGGATGAGGCACA
PRIMER_INTERNAL_4_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA
PRIMER_LEFT_4=108,20
PRIMER_RIGHT_4=187,21
PRIMER_INTERNAL_4=130,25
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=59.940
PRIMER_INTERNAL_4_TM=60.051
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=28.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=9.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH162
SEQUENCE_TEMPLATE=ATGGAGATGTTGTCACCTGGCATTATATGCTATAATNCTTGGTCACATTTATAAGTTTTTTTATTCTAATACAATGGATCTGAGTNTCATTAGGAGCCTTGCAGACAAGTGTTTCTGGGTAACTTCTCAGTAACATCTTTGACATTTGTTTAGTTGCAGACCTAAATGGCCACTGATGGATACTGCAGTTTCAGTAAGCAGTGGAGTTTATTTCCCTGG
SEQUENCE_INCLUDED_REGION=0,219
P3_COMMENT=CL is AGATGTTGTCACCTGGCATT
P3_COMMENT=CR is ACTCCACTGCTTACTGAAACTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.313697
PRIMER_LEFT_0_PENALTY=0.127097
PRIMER_RIGHT_0_PENALTY=0.186600
PRIMER_INTERNAL_0_PENALTY=5.416361
PRIMER_LEFT_0_SEQUENCE=TGCAGACAAGTGTTTCTGGG
PRIMER_RIGHT_0_SEQUENCE=CCATCAGTGGCCATTTAGGT
PRIMER_INTERNAL_0_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA
PRIMER_LEFT_0=99,20
PRIMER_RIGHT_0=178,20
PRIMER_INTERNAL_0=135,23
PRIMER_LEFT_0_TM=59.873
PRIMER_RIGHT_0_TM=59.813
PRIMER_INTERNAL_0_TM=57.584
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=30.435
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=0.639932
PRIMER_LEFT_1_PENALTY=0.453332
PRIMER_RIGHT_1_PENALTY=0.186600
PRIMER_INTERNAL_1_PENALTY=5.416361
PRIMER_LEFT_1_SEQUENCE=GGAGCCTTGCAGACAAGTGT
PRIMER_RIGHT_1_SEQUENCE=CCATCAGTGGCCATTTAGGT
PRIMER_INTERNAL_1_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA
PRIMER_LEFT_1=92,20
PRIMER_RIGHT_1=178,20
PRIMER_INTERNAL_1=135,23
PRIMER_LEFT_1_TM=60.453
PRIMER_RIGHT_1_TM=59.813
PRIMER_INTERNAL_1_TM=57.584
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=30.435
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=0.775568
PRIMER_LEFT_2_PENALTY=0.588967
PRIMER_RIGHT_2_PENALTY=0.186600
PRIMER_INTERNAL_2_PENALTY=5.416361
PRIMER_LEFT_2_SEQUENCE=AGGAGCCTTGCAGACAAGTG
PRIMER_RIGHT_2_SEQUENCE=CCATCAGTGGCCATTTAGGT
PRIMER_INTERNAL_2_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA
PRIMER_LEFT_2=91,20
PRIMER_RIGHT_2=178,20
PRIMER_INTERNAL_2=135,23
PRIMER_LEFT_2_TM=60.589
PRIMER_RIGHT_2_TM=59.813
PRIMER_INTERNAL_2_TM=57.584
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=30.435
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=0.784190
PRIMER_LEFT_3_PENALTY=0.127097
PRIMER_RIGHT_3_PENALTY=0.657094
PRIMER_INTERNAL_3_PENALTY=7.414202
PRIMER_LEFT_3_SEQUENCE=TGCAGACAAGTGTTTCTGGG
PRIMER_RIGHT_3_SEQUENCE=AGTGGCCATTTAGGTCTGCA
PRIMER_INTERNAL_3_SEQUENCE=TTCTCAGTAACATCTTTGACATTTG
PRIMER_LEFT_3=99,20
PRIMER_RIGHT_3=173,20
PRIMER_INTERNAL_3=123,25
PRIMER_LEFT_3_TM=59.873
PRIMER_RIGHT_3_TM=60.657
PRIMER_INTERNAL_3_TM=57.586
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=32.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=0.784190
PRIMER_LEFT_4_PENALTY=0.127097
PRIMER_RIGHT_4_PENALTY=0.657094
PRIMER_INTERNAL_4_PENALTY=7.414202
PRIMER_LEFT_4_SEQUENCE=TGCAGACAAGTGTTTCTGGG
PRIMER_RIGHT_4_SEQUENCE=CAGTGGCCATTTAGGTCTGC
PRIMER_INTERNAL_4_SEQUENCE=TTCTCAGTAACATCTTTGACATTTG
PRIMER_LEFT_4=99,20
PRIMER_RIGHT_4=174,20
PRIMER_INTERNAL_4=123,25
PRIMER_LEFT_4_TM=59.873
PRIMER_RIGHT_4_TM=60.657
PRIMER_INTERNAL_4_TM=57.586
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH164
SEQUENCE_TEMPLATE=CTACACCACCCAAAATGAGCAGTTTCTCTAAAGAGATTGGGTTCAGTAAAGGTCAGTCCTACTTCAATTTCAGTCTGGCCCTTCTCATTCTTATGTTCTACTTAAATATTTATCTTCTATTTTCGTACCAAAATTATTAGATGTTTCTTAGTTTACATTAAGTTTCTTTTAACTGGTAACACGTGAGGATTTTCATAGACAATTTTTTCTTTTGAACAAAATTGTCAATATGTCTT
SEQUENCE_INCLUDED_REGION=0,236
P3_COMMENT=CL is CTACACCACCCAAAATGAGC
P3_COMMENT=CR is AATTGTCTATGAAAATCCTCACG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.226156
PRIMER_LEFT_0_PENALTY=0.029253
PRIMER_RIGHT_0_PENALTY=0.196903
PRIMER_INTERNAL_0_PENALTY=2.394672
PRIMER_LEFT_0_SEQUENCE=CCACCCAAAATGAGCAGTTT
PRIMER_RIGHT_0_SEQUENCE=ATGAGAAGGGCCAGACTGAA
PRIMER_INTERNAL_0_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
PRIMER_LEFT_0=5,20
PRIMER_RIGHT_0=87,20
PRIMER_INTERNAL_0=37,22
PRIMER_LEFT_0_TM=59.971
PRIMER_RIGHT_0_TM=59.803
PRIMER_INTERNAL_0_TM=60.395
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=83
PRIMER_PAIR_1_PENALTY=0.226156
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.196903
PRIMER_INTERNAL_1_PENALTY=2.394672
PRIMER_LEFT_1_SEQUENCE=CCACCCAAAATGAGCAGTTT
PRIMER_RIGHT_1_SEQUENCE=AATGAGAAGGGCCAGACTGA
PRIMER_INTERNAL_1_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
PRIMER_LEFT_1=5,20
PRIMER_RIGHT_1=88,20
PRIMER_INTERNAL_1=37,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.803
PRIMER_INTERNAL_1_TM=60.395
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=0.226156
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.196903
PRIMER_INTERNAL_2_PENALTY=2.394672
PRIMER_LEFT_2_SEQUENCE=CCACCCAAAATGAGCAGTTT
PRIMER_RIGHT_2_SEQUENCE=GAATGAGAAGGGCCAGACTG
PRIMER_INTERNAL_2_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
PRIMER_LEFT_2=5,20
PRIMER_RIGHT_2=89,20
PRIMER_INTERNAL_2=37,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=59.803
PRIMER_INTERNAL_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=0.232446
PRIMER_LEFT_3_PENALTY=0.035543
PRIMER_RIGHT_3_PENALTY=0.196903
PRIMER_INTERNAL_3_PENALTY=2.394672
PRIMER_LEFT_3_SEQUENCE=TACACCACCCAAAATGAGCA
PRIMER_RIGHT_3_SEQUENCE=ATGAGAAGGGCCAGACTGAA
PRIMER_INTERNAL_3_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=87,20
PRIMER_INTERNAL_3=37,22
PRIMER_LEFT_3_TM=59.964
PRIMER_RIGHT_3_TM=59.803
PRIMER_INTERNAL_3_TM=60.395
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=87
PRIMER_PAIR_4_PENALTY=0.232446
PRIMER_LEFT_4_PENALTY=0.035543
PRIMER_RIGHT_4_PENALTY=0.196903
PRIMER_INTERNAL_4_PENALTY=2.394672
PRIMER_LEFT_4_SEQUENCE=TACACCACCCAAAATGAGCA
PRIMER_RIGHT_4_SEQUENCE=AATGAGAAGGGCCAGACTGA
PRIMER_INTERNAL_4_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=88,20
PRIMER_INTERNAL_4=37,22
PRIMER_LEFT_4_TM=59.964
PRIMER_RIGHT_4_TM=59.803
PRIMER_INTERNAL_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH168
SEQUENCE_TEMPLATE=CTTAGAAATAGAAGAGGCTTCCTCCCTCTAATGGTTCCACTCAAGTNCCTATCTGGTTCCAAGAGGTCTTCTTTTTGGATTAACAAAAATTAGTTCCAAAAGTGAGAAATTGTGCATCTTGTGGAAATGAGAGAACCACTGGCTATGCCTAGAAAATGTATCCTTGGAGGCACTAGGAAAATGGCAGTAAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAT
SEQUENCE_INCLUDED_REGION=0,189
P3_COMMENT=CL is GAAATAGAAGAGGCTTCCTCCC
P3_COMMENT=CR is CCATTTTCCTAGTGCCTCCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.170026
PRIMER_LEFT_0_PENALTY=1.103702
PRIMER_RIGHT_0_PENALTY=0.066323
PRIMER_INTERNAL_0_PENALTY=0.687142
PRIMER_LEFT_0_SEQUENCE=TTGTGCATCTTGTGGAAATGA
PRIMER_RIGHT_0_SEQUENCE=CCATTTTCCTAGTGCCTCCA
PRIMER_INTERNAL_0_SEQUENCE=GAGAACCACTGGCTATGCCT
PRIMER_LEFT_0=109,21
PRIMER_RIGHT_0=183,20
PRIMER_INTERNAL_0=130,20
PRIMER_LEFT_0_TM=60.104
PRIMER_RIGHT_0_TM=60.066
PRIMER_INTERNAL_0_TM=59.313
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=1.317208
PRIMER_LEFT_1_PENALTY=1.103702
PRIMER_RIGHT_1_PENALTY=0.213506
PRIMER_INTERNAL_1_PENALTY=0.687142
PRIMER_LEFT_1_SEQUENCE=TTGTGCATCTTGTGGAAATGA
PRIMER_RIGHT_1_SEQUENCE=TGCCATTTTCCTAGTGCCTC
PRIMER_INTERNAL_1_SEQUENCE=GAGAACCACTGGCTATGCCT
PRIMER_LEFT_1=109,21
PRIMER_RIGHT_1=185,20
PRIMER_INTERNAL_1=130,20
PRIMER_LEFT_1_TM=60.104
PRIMER_RIGHT_1_TM=60.214
PRIMER_INTERNAL_1_TM=59.313
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=1.506940
PRIMER_LEFT_2_PENALTY=1.293434
PRIMER_RIGHT_2_PENALTY=0.213506
PRIMER_INTERNAL_2_PENALTY=1.629732
PRIMER_LEFT_2_SEQUENCE=TGTGCATCTTGTGGAAATGAG
PRIMER_RIGHT_2_SEQUENCE=TGCCATTTTCCTAGTGCCTC
PRIMER_INTERNAL_2_SEQUENCE=CCACTGGCTATGCCTAGAAAA
PRIMER_LEFT_2=110,21
PRIMER_RIGHT_2=185,20
PRIMER_INTERNAL_2=135,21
PRIMER_LEFT_2_TM=59.707
PRIMER_RIGHT_2_TM=60.214
PRIMER_INTERNAL_2_TM=59.370
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=1.593388
PRIMER_LEFT_3_PENALTY=1.379883
PRIMER_RIGHT_3_PENALTY=0.213506
PRIMER_INTERNAL_3_PENALTY=0.687142
PRIMER_LEFT_3_SEQUENCE=TGTGCATCTTGTGGAAATGA
PRIMER_RIGHT_3_SEQUENCE=TGCCATTTTCCTAGTGCCTC
PRIMER_INTERNAL_3_SEQUENCE=GAGAACCACTGGCTATGCCT
PRIMER_LEFT_3=110,20
PRIMER_RIGHT_3=185,20
PRIMER_INTERNAL_3=130,20
PRIMER_LEFT_3_TM=58.620
PRIMER_RIGHT_3_TM=60.214
PRIMER_INTERNAL_3_TM=59.313
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=1.768027
PRIMER_LEFT_4_PENALTY=1.103702
PRIMER_RIGHT_4_PENALTY=0.664324
PRIMER_INTERNAL_4_PENALTY=0.687142
PRIMER_LEFT_4_SEQUENCE=TTGTGCATCTTGTGGAAATGA
PRIMER_RIGHT_4_SEQUENCE=CTGCCATTTTCCTAGTGCCT
PRIMER_INTERNAL_4_SEQUENCE=GAGAACCACTGGCTATGCCT
PRIMER_LEFT_4=109,21
PRIMER_RIGHT_4=186,20
PRIMER_INTERNAL_4=130,20
PRIMER_LEFT_4_TM=60.104
PRIMER_RIGHT_4_TM=59.336
PRIMER_INTERNAL_4_TM=59.313
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH170
SEQUENCE_TEMPLATE=CTCGATCTTCAGAAAGTGACTCTATCAAATCTGAAGGACACCCAAATTTAAAAAAGTATCATGTAATTAAAAAAACCACTTTGCATGAATGTATCTTTTNCACTTTAAAAAATTAAAAAAAATAGTACANNGCAGTACANACCTTTATCCCGTTCTTGTTGCTGCATTGTACTTTTCAACTTATCACTAAGNTCATTTATTATGTTTTCTTTTCTCATTTTCTCTCTTGTTAAAACAGTGTTAAAATTGG
SEQUENCE_INCLUDED_REGION=0,250
P3_COMMENT=CL is CTATCAAATCTGAAGGACACCC
P3_COMMENT=CR is CACTGTTTTAACAAGAGAGAAAATG
P3_COMMENT=CM is CATGTAATTAAAAAAACCACTTTGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.866629
PRIMER_LEFT_0_PENALTY=5.899214
PRIMER_RIGHT_0_PENALTY=2.967416
PRIMER_INTERNAL_0_PENALTY=2.917484
PRIMER_LEFT_0_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA
PRIMER_RIGHT_0_SEQUENCE=CATTCATGCAAAGTGGTTTTTT
PRIMER_INTERNAL_0_SEQUENCE=TCTGAAGGACACCCAAATTT
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=90,22
PRIMER_INTERNAL_0=29,20
PRIMER_LEFT_0_TM=59.101
PRIMER_RIGHT_0_TM=59.033
PRIMER_INTERNAL_0_TM=57.083
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=31.818
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=8.958627
PRIMER_LEFT_1_PENALTY=5.991211
PRIMER_RIGHT_1_PENALTY=2.967416
PRIMER_INTERNAL_1_PENALTY=8.915111
PRIMER_LEFT_1_SEQUENCE=TGACTCTATCAAATCTGAAGGACA
PRIMER_RIGHT_1_SEQUENCE=CATTCATGCAAAGTGGTTTTTT
PRIMER_INTERNAL_1_SEQUENCE=CCCAAATTTAAAAAAGTATCATGTAA
PRIMER_LEFT_1=16,24
PRIMER_RIGHT_1=90,22
PRIMER_INTERNAL_1=40,26
PRIMER_LEFT_1_TM=58.009
PRIMER_RIGHT_1_TM=59.033
PRIMER_INTERNAL_1_TM=57.085
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=31.818
PRIMER_INTERNAL_1_GC_PERCENT=23.077
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=9.093109
PRIMER_LEFT_2_PENALTY=5.899214
PRIMER_RIGHT_2_PENALTY=3.193896
PRIMER_INTERNAL_2_PENALTY=2.917484
PRIMER_LEFT_2_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA
PRIMER_RIGHT_2_SEQUENCE=ACATTCATGCAAAGTGGTTTTTT
PRIMER_INTERNAL_2_SEQUENCE=TCTGAAGGACACCCAAATTT
PRIMER_LEFT_2=2,25
PRIMER_RIGHT_2=91,23
PRIMER_INTERNAL_2=29,20
PRIMER_LEFT_2_TM=59.101
PRIMER_RIGHT_2_TM=59.806
PRIMER_INTERNAL_2_TM=57.083
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=9.127334
PRIMER_LEFT_3_PENALTY=6.159919
PRIMER_RIGHT_3_PENALTY=2.967416
PRIMER_INTERNAL_3_PENALTY=8.915111
PRIMER_LEFT_3_SEQUENCE=GTGACTCTATCAAATCTGAAGGACA
PRIMER_RIGHT_3_SEQUENCE=CATTCATGCAAAGTGGTTTTTT
PRIMER_INTERNAL_3_SEQUENCE=CCCAAATTTAAAAAAGTATCATGTAA
PRIMER_LEFT_3=15,25
PRIMER_RIGHT_3=90,22
PRIMER_INTERNAL_3=40,26
PRIMER_LEFT_3_TM=58.840
PRIMER_RIGHT_3_TM=59.033
PRIMER_INTERNAL_3_TM=57.085
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=31.818
PRIMER_INTERNAL_3_GC_PERCENT=23.077
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=9.156599
PRIMER_LEFT_4_PENALTY=5.899214
PRIMER_RIGHT_4_PENALTY=3.257385
PRIMER_INTERNAL_4_PENALTY=2.917484
PRIMER_LEFT_4_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA
PRIMER_RIGHT_4_SEQUENCE=CATTCATGCAAAGTGGTTTTTTT
PRIMER_INTERNAL_4_SEQUENCE=TCTGAAGGACACCCAAATTT
PRIMER_LEFT_4=2,25
PRIMER_RIGHT_4=90,23
PRIMER_INTERNAL_4=29,20
PRIMER_LEFT_4_TM=59.101
PRIMER_RIGHT_4_TM=60.257
PRIMER_INTERNAL_4_TM=57.083
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=30.435
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH176
SEQUENCE_TEMPLATE=CTGGTATAAAGGCCCCTTTGGATGANATTNACCCCAACCAGACTAATGGGAGTNCAGAAGGGATGCTGTGGGTCCCCCACATCNTCAGAATGCCAACGGGACCTGGGGGTCCTGTTAATCTTTTTAGCCAGACTGAATTCCATTCTGTTCTTATAAAATCCC
SEQUENCE_INCLUDED_REGION=0,162
P3_COMMENT=CL is CTGGTATAAAGGCCCCTTTG
P3_COMMENT=CR is AAGATTAACAGGACCCCCAG
P3_COMMENT=CM is ACCCCAACCAGACTAATGGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.651395
PRIMER_LEFT_0_PENALTY=0.599485
PRIMER_RIGHT_0_PENALTY=0.051910
PRIMER_INTERNAL_0_PENALTY=1.064190
PRIMER_LEFT_0_SEQUENCE=CCCAACCAGACTAATGGGAG
PRIMER_RIGHT_0_SEQUENCE=ATTAACAGGACCCCCAGGTC
PRIMER_INTERNAL_0_SEQUENCE=AGAAGGGATGCTGTGGGTC
PRIMER_LEFT_0=32,20
PRIMER_RIGHT_0=118,20
PRIMER_INTERNAL_0=55,19
PRIMER_LEFT_0_TM=59.401
PRIMER_RIGHT_0_TM=60.052
PRIMER_INTERNAL_0_TM=60.064
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=0.651395
PRIMER_LEFT_1_PENALTY=0.599485
PRIMER_RIGHT_1_PENALTY=0.051910
PRIMER_INTERNAL_1_PENALTY=1.064190
PRIMER_LEFT_1_SEQUENCE=CCCAACCAGACTAATGGGAG
PRIMER_RIGHT_1_SEQUENCE=GATTAACAGGACCCCCAGGT
PRIMER_INTERNAL_1_SEQUENCE=AGAAGGGATGCTGTGGGTC
PRIMER_LEFT_1=32,20
PRIMER_RIGHT_1=119,20
PRIMER_INTERNAL_1=55,19
PRIMER_LEFT_1_TM=59.401
PRIMER_RIGHT_1_TM=60.052
PRIMER_INTERNAL_1_TM=60.064
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=0.782143
PRIMER_LEFT_2_PENALTY=0.599485
PRIMER_RIGHT_2_PENALTY=0.182658
PRIMER_INTERNAL_2_PENALTY=1.064190
PRIMER_LEFT_2_SEQUENCE=CCCAACCAGACTAATGGGAG
PRIMER_RIGHT_2_SEQUENCE=AGATTAACAGGACCCCCAGG
PRIMER_INTERNAL_2_SEQUENCE=AGAAGGGATGCTGTGGGTC
PRIMER_LEFT_2=32,20
PRIMER_RIGHT_2=120,20
PRIMER_INTERNAL_2=55,19
PRIMER_LEFT_2_TM=59.401
PRIMER_RIGHT_2_TM=60.183
PRIMER_INTERNAL_2_TM=60.064
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=1.039931
PRIMER_LEFT_3_PENALTY=0.988020
PRIMER_RIGHT_3_PENALTY=0.051910
PRIMER_INTERNAL_3_PENALTY=1.064190
PRIMER_LEFT_3_SEQUENCE=ACCCCAACCAGACTAATGGG
PRIMER_RIGHT_3_SEQUENCE=ATTAACAGGACCCCCAGGTC
PRIMER_INTERNAL_3_SEQUENCE=AGAAGGGATGCTGTGGGTC
PRIMER_LEFT_3=30,20
PRIMER_RIGHT_3=118,20
PRIMER_INTERNAL_3=55,19
PRIMER_LEFT_3_TM=60.988
PRIMER_RIGHT_3_TM=60.052
PRIMER_INTERNAL_3_TM=60.064
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=1.039931
PRIMER_LEFT_4_PENALTY=0.988020
PRIMER_RIGHT_4_PENALTY=0.051910
PRIMER_INTERNAL_4_PENALTY=1.064190
PRIMER_LEFT_4_SEQUENCE=ACCCCAACCAGACTAATGGG
PRIMER_RIGHT_4_SEQUENCE=GATTAACAGGACCCCCAGGT
PRIMER_INTERNAL_4_SEQUENCE=AGAAGGGATGCTGTGGGTC
PRIMER_LEFT_4=30,20
PRIMER_RIGHT_4=119,20
PRIMER_INTERNAL_4=55,19
PRIMER_LEFT_4_TM=60.988
PRIMER_RIGHT_4_TM=60.052
PRIMER_INTERNAL_4_TM=60.064
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH177
SEQUENCE_TEMPLATE=CTTTAGATATATTTAAATCATTTGCCATCAGAAGTCTGTACTGTAGGTCTTACTATTATCCTCATTTTACAGATGAAGCACAAAGATCCATAGTCACATATTTTGTAACAAGAGCAGGATCTGCAGCCAGGCAGTCTGGGTCCATGGTCCATGTTCTTACCCATGGTGCAATACTGACTCTTGAAGTCCAGGGTCTACTAAGAATCCAGGTCTACCTGACTCCAAAG
SEQUENCE_INCLUDED_REGION=0,227
P3_COMMENT=CL is CATTTGCCATCAGAAGTCTG
P3_COMMENT=CR is TGGAGTCAGGTAGACCTGGA
P3_COMMENT=CM is ATTATCCTCATTTTACAGATGAAGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.657623
PRIMER_LEFT_0_PENALTY=0.363753
PRIMER_RIGHT_0_PENALTY=1.293870
PRIMER_INTERNAL_0_PENALTY=1.390393
PRIMER_LEFT_0_SEQUENCE=GGTCCATGGTCCATGTTCTT
PRIMER_RIGHT_0_SEQUENCE=TTGGAGTCAGGTAGACCTGGA
PRIMER_INTERNAL_0_SEQUENCE=CCCATGGTGCAATACTGACTC
PRIMER_LEFT_0=138,20
PRIMER_RIGHT_0=224,21
PRIMER_INTERNAL_0=159,21
PRIMER_LEFT_0_TM=59.636
PRIMER_RIGHT_0_TM=59.706
PRIMER_INTERNAL_0_TM=60.390
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=1.842081
PRIMER_LEFT_1_PENALTY=0.035543
PRIMER_RIGHT_1_PENALTY=1.806538
PRIMER_INTERNAL_1_PENALTY=1.710351
PRIMER_LEFT_1_SEQUENCE=TGTTCTTACCCATGGTGCAA
PRIMER_RIGHT_1_SEQUENCE=TTTGGAGTCAGGTAGACCTGG
PRIMER_INTERNAL_1_SEQUENCE=TGACTCTTGAAGTCCAGGGTC
PRIMER_LEFT_1=151,20
PRIMER_RIGHT_1=225,21
PRIMER_INTERNAL_1=174,21
PRIMER_LEFT_1_TM=59.964
PRIMER_RIGHT_1_TM=59.193
PRIMER_INTERNAL_1_TM=59.290
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=2.147662
PRIMER_LEFT_2_PENALTY=0.363753
PRIMER_RIGHT_2_PENALTY=1.783908
PRIMER_INTERNAL_2_PENALTY=1.390393
PRIMER_LEFT_2_SEQUENCE=GGTCCATGGTCCATGTTCTT
PRIMER_RIGHT_2_SEQUENCE=TGGAGTCAGGTAGACCTGGA
PRIMER_INTERNAL_2_SEQUENCE=CCCATGGTGCAATACTGACTC
PRIMER_LEFT_2=138,20
PRIMER_RIGHT_2=223,20
PRIMER_INTERNAL_2=159,21
PRIMER_LEFT_2_TM=59.636
PRIMER_RIGHT_2_TM=58.216
PRIMER_INTERNAL_2_TM=60.390
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=2.170291
PRIMER_LEFT_3_PENALTY=0.363753
PRIMER_RIGHT_3_PENALTY=1.806538
PRIMER_INTERNAL_3_PENALTY=1.390393
PRIMER_LEFT_3_SEQUENCE=GGTCCATGGTCCATGTTCTT
PRIMER_RIGHT_3_SEQUENCE=TTTGGAGTCAGGTAGACCTGG
PRIMER_INTERNAL_3_SEQUENCE=CCCATGGTGCAATACTGACTC
PRIMER_LEFT_3=138,20
PRIMER_RIGHT_3=225,21
PRIMER_INTERNAL_3=159,21
PRIMER_LEFT_3_TM=59.636
PRIMER_RIGHT_3_TM=59.193
PRIMER_INTERNAL_3_TM=60.390
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=9.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=2.195208
PRIMER_LEFT_4_PENALTY=0.035543
PRIMER_RIGHT_4_PENALTY=2.159665
PRIMER_INTERNAL_4_PENALTY=1.710351
PRIMER_LEFT_4_SEQUENCE=TGTTCTTACCCATGGTGCAA
PRIMER_RIGHT_4_SEQUENCE=CTTTGGAGTCAGGTAGACCTGG
PRIMER_INTERNAL_4_SEQUENCE=TGACTCTTGAAGTCCAGGGTC
PRIMER_LEFT_4=151,20
PRIMER_RIGHT_4=226,22
PRIMER_INTERNAL_4=174,21
PRIMER_LEFT_4_TM=59.964
PRIMER_RIGHT_4_TM=60.160
PRIMER_INTERNAL_4_TM=59.290
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH184
SEQUENCE_TEMPLATE=CTACATTTCAAACCGGAATCATTGGCTCAGTNTGGATTCTCCAGGAAGCAGGCCTGAGGCAAAGGCTGATGTGCTAACACATCAATAGAGTNTATAGTCTTAGGGGCAACAATAGGGAATGAGGTGAGAAAGACAGGTAAGGAGACAGACTCAATAGGAAGATGTGTCATCAAGCAGGACACT
SEQUENCE_INCLUDED_REGION=0,183
P3_COMMENT=CL is CAAAGGCTGATGTGCTAACA
P3_COMMENT=CR is GTGTCCTGCTTGATGACACA
P3_COMMENT=CM is TATAGTCTTAGGGGCAACAATAGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.692117
PRIMER_LEFT_0_PENALTY=1.098301
PRIMER_RIGHT_0_PENALTY=0.593816
PRIMER_INTERNAL_0_PENALTY=0.934426
PRIMER_LEFT_0_SEQUENCE=GATTCTCCAGGAAGCAGGC
PRIMER_RIGHT_0_SEQUENCE=TTCCCTATTGTTGCCCCTAA
PRIMER_INTERNAL_0_SEQUENCE=AGGCAAAGGCTGATGTGCTA
PRIMER_LEFT_0=34,19
PRIMER_RIGHT_0=118,20
PRIMER_INTERNAL_0=56,20
PRIMER_LEFT_0_TM=59.902
PRIMER_RIGHT_0_TM=59.406
PRIMER_INTERNAL_0_TM=60.934
PRIMER_LEFT_0_GC_PERCENT=57.895
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=1.711520
PRIMER_LEFT_1_PENALTY=1.098301
PRIMER_RIGHT_1_PENALTY=0.613219
PRIMER_INTERNAL_1_PENALTY=0.934426
PRIMER_LEFT_1_SEQUENCE=GATTCTCCAGGAAGCAGGC
PRIMER_RIGHT_1_SEQUENCE=CTCATTCCCTATTGTTGCCC
PRIMER_INTERNAL_1_SEQUENCE=AGGCAAAGGCTGATGTGCTA
PRIMER_LEFT_1=34,19
PRIMER_RIGHT_1=122,20
PRIMER_INTERNAL_1=56,20
PRIMER_LEFT_1_TM=59.902
PRIMER_RIGHT_1_TM=59.387
PRIMER_INTERNAL_1_TM=60.934
PRIMER_LEFT_1_GC_PERCENT=57.895
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=89
PRIMER_PAIR_2_PENALTY=1.711520
PRIMER_LEFT_2_PENALTY=1.098301
PRIMER_RIGHT_2_PENALTY=0.613219
PRIMER_INTERNAL_2_PENALTY=0.934426
PRIMER_LEFT_2_SEQUENCE=GATTCTCCAGGAAGCAGGC
PRIMER_RIGHT_2_SEQUENCE=CCTCATTCCCTATTGTTGCC
PRIMER_INTERNAL_2_SEQUENCE=AGGCAAAGGCTGATGTGCTA
PRIMER_LEFT_2=34,19
PRIMER_RIGHT_2=123,20
PRIMER_INTERNAL_2=56,20
PRIMER_LEFT_2_TM=59.902
PRIMER_RIGHT_2_TM=59.387
PRIMER_INTERNAL_2_TM=60.934
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=1.847639
PRIMER_LEFT_3_PENALTY=1.253823
PRIMER_RIGHT_3_PENALTY=0.593816
PRIMER_INTERNAL_3_PENALTY=0.934426
PRIMER_LEFT_3_SEQUENCE=GGATTCTCCAGGAAGCAGG
PRIMER_RIGHT_3_SEQUENCE=TTCCCTATTGTTGCCCCTAA
PRIMER_INTERNAL_3_SEQUENCE=AGGCAAAGGCTGATGTGCTA
PRIMER_LEFT_3=33,19
PRIMER_RIGHT_3=118,20
PRIMER_INTERNAL_3=56,20
PRIMER_LEFT_3_TM=59.746
PRIMER_RIGHT_3_TM=59.406
PRIMER_INTERNAL_3_TM=60.934
PRIMER_LEFT_3_GC_PERCENT=57.895
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=1.867041
PRIMER_LEFT_4_PENALTY=1.253823
PRIMER_RIGHT_4_PENALTY=0.613219
PRIMER_INTERNAL_4_PENALTY=0.934426
PRIMER_LEFT_4_SEQUENCE=GGATTCTCCAGGAAGCAGG
PRIMER_RIGHT_4_SEQUENCE=CTCATTCCCTATTGTTGCCC
PRIMER_INTERNAL_4_SEQUENCE=AGGCAAAGGCTGATGTGCTA
PRIMER_LEFT_4=33,19
PRIMER_RIGHT_4=122,20
PRIMER_INTERNAL_4=56,20
PRIMER_LEFT_4_TM=59.746
PRIMER_RIGHT_4_TM=59.387
PRIMER_INTERNAL_4_TM=60.934
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH192
SEQUENCE_TEMPLATE=AGTGGCCTGATGATGGCTCACTGTAACGTAACTCCTGGCCTCAGCCTCCAGAGTNNCTGGAACTATAGGTACACGCCAACACACCAGGGTAATTTTTTTTTTTTTTTTTTGTAGAGACAGGGTCTCGCTATGTTGCCCACTCTGTTCTATTTTGAAGGTACAACCAACAAGCTTTCTTGACAGNTTGAAAGTGAAAATGAGAGTATGAGAGGAGTTATGGATGATATAAAGTTTTTGGCCTGAGTAACTAGAAATCCTCGCCATCAACCTAGAATGGGGAAGACCATACATTACTTACAGGTTCG
SEQUENCE_INCLUDED_REGION=0,305
P3_COMMENT=CL is GCTATGTTGCCCACTCTGTT
P3_COMMENT=CR is ATGGTCTTCCCCATTCTAGG
P3_COMMENT=CM is TATTTTGAAGGTACAACCAACAAG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.795044
PRIMER_LEFT_0_PENALTY=0.509923
PRIMER_RIGHT_0_PENALTY=0.285121
PRIMER_INTERNAL_0_PENALTY=7.399766
PRIMER_LEFT_0_SEQUENCE=TAGGTACACGCCAACACACC
PRIMER_RIGHT_0_SEQUENCE=AGTGGGCAACATAGCGAGAC
PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTGTAGAGACAGG
PRIMER_LEFT_0=65,20
PRIMER_RIGHT_0=140,20
PRIMER_INTERNAL_0=94,27
PRIMER_LEFT_0_TM=59.490
PRIMER_RIGHT_0_TM=60.285
PRIMER_INTERNAL_0_TM=59.600
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=22.222
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=0.928609
PRIMER_LEFT_1_PENALTY=0.509923
PRIMER_RIGHT_1_PENALTY=0.418685
PRIMER_INTERNAL_1_PENALTY=5.782983
PRIMER_LEFT_1_SEQUENCE=TAGGTACACGCCAACACACC
PRIMER_RIGHT_1_SEQUENCE=AGAGTGGGCAACATAGCGAG
PRIMER_INTERNAL_1_SEQUENCE=TTTTTTTTTTTTTGTAGAGACAGGG
PRIMER_LEFT_1=65,20
PRIMER_RIGHT_1=142,20
PRIMER_INTERNAL_1=97,25
PRIMER_LEFT_1_TM=59.490
PRIMER_RIGHT_1_TM=60.419
PRIMER_INTERNAL_1_TM=59.217
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=28.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=1.490526
PRIMER_LEFT_2_PENALTY=1.205406
PRIMER_RIGHT_2_PENALTY=0.285121
PRIMER_INTERNAL_2_PENALTY=7.399766
PRIMER_LEFT_2_SEQUENCE=ATAGGTACACGCCAACACACC
PRIMER_RIGHT_2_SEQUENCE=AGTGGGCAACATAGCGAGAC
PRIMER_INTERNAL_2_SEQUENCE=TTTTTTTTTTTTTTTTGTAGAGACAGG
PRIMER_LEFT_2=64,21
PRIMER_RIGHT_2=140,20
PRIMER_INTERNAL_2=94,27
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=60.285
PRIMER_INTERNAL_2_TM=59.600
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=22.222
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=1.546608
PRIMER_LEFT_3_PENALTY=1.263740
PRIMER_RIGHT_3_PENALTY=0.282868
PRIMER_INTERNAL_3_PENALTY=0.647368
PRIMER_LEFT_3_SEQUENCE=GCCTGATGATGGCTCACTGT
PRIMER_RIGHT_3_SEQUENCE=AATTACCCTGGTGTGTTGGC
PRIMER_INTERNAL_3_SEQUENCE=AACGTAACTCCTGGCCTCAG
PRIMER_LEFT_3=4,20
PRIMER_RIGHT_3=93,20
PRIMER_INTERNAL_3=24,20
PRIMER_LEFT_3_TM=61.264
PRIMER_RIGHT_3_TM=59.717
PRIMER_INTERNAL_3_TM=59.353
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=1.551977
PRIMER_LEFT_4_PENALTY=1.269110
PRIMER_RIGHT_4_PENALTY=0.282868
PRIMER_INTERNAL_4_PENALTY=1.041745
PRIMER_LEFT_4_SEQUENCE=TGATGATGGCTCACTGTAACG
PRIMER_RIGHT_4_SEQUENCE=AATTACCCTGGTGTGTTGGC
PRIMER_INTERNAL_4_SEQUENCE=TAACTCCTGGCCTCAGCCT
PRIMER_LEFT_4=7,21
PRIMER_RIGHT_4=93,20
PRIMER_INTERNAL_4=28,19
PRIMER_LEFT_4_TM=59.731
PRIMER_RIGHT_4_TM=59.717
PRIMER_INTERNAL_4_TM=59.958
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH193
SEQUENCE_TEMPLATE=CTACCACGCCCTGCTCTCCATTCACTCTTGAACTTACTCCAAGCAGACATTTGTCCCCGCCATTCCACAAATAACCTTCATGTAGCCAACTCCAGGAGTNAATACTTTTCCTAAGTCTGTTTACTGGATTTCATCCTTTTTTCAACCTCTAAAAATTTCAGTGCCCTGGGATCTCTGGGTNTCTTTCCCTTGACAATTTCCTCTAATGCTGTGGCTTTTTAGACCACCTTTATGATGATGACATCAAAATTCCTATCCCACTTCTCCTCTGAGGTCCAGTCCCCAATCCCCACTGGAATGTTTAATAGATATATTA
SEQUENCE_INCLUDED_REGION=0,316
P3_COMMENT=CL is CTACCACGCCCTGCTCTC
P3_COMMENT=CR is TAAACATTCCAGTGGGGATT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.889487
PRIMER_LEFT_0_PENALTY=0.536418
PRIMER_RIGHT_0_PENALTY=0.353069
PRIMER_INTERNAL_0_PENALTY=0.648069
PRIMER_LEFT_0_SEQUENCE=TGCTGTGGCTTTTTAGACCA
PRIMER_RIGHT_0_SEQUENCE=AAACATTCCAGTGGGGATTG
PRIMER_INTERNAL_0_SEQUENCE=TCTCCTCTGAGGTCCAGTCC
PRIMER_LEFT_0=206,20
PRIMER_RIGHT_0=302,20
PRIMER_INTERNAL_0=262,20
PRIMER_LEFT_0_TM=59.464
PRIMER_RIGHT_0_TM=59.647
PRIMER_INTERNAL_0_TM=59.352
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=1.184487
PRIMER_LEFT_1_PENALTY=0.536418
PRIMER_RIGHT_1_PENALTY=0.648069
PRIMER_INTERNAL_1_PENALTY=2.726686
PRIMER_LEFT_1_SEQUENCE=TGCTGTGGCTTTTTAGACCA
PRIMER_RIGHT_1_SEQUENCE=GGACTGGACCTCAGAGGAGA
PRIMER_INTERNAL_1_SEQUENCE=TGACATCAAAATTCCTATCCCA
PRIMER_LEFT_1=206,20
PRIMER_RIGHT_1=281,20
PRIMER_INTERNAL_1=238,22
PRIMER_LEFT_1_TM=59.464
PRIMER_RIGHT_1_TM=59.352
PRIMER_INTERNAL_1_TM=59.273
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=1.354362
PRIMER_LEFT_2_PENALTY=0.595208
PRIMER_RIGHT_2_PENALTY=0.759154
PRIMER_INTERNAL_2_PENALTY=0.177008
PRIMER_LEFT_2_SEQUENCE=CCCTGCTCTCCATTCACTCT
PRIMER_RIGHT_2_SEQUENCE=TCCTGGAGTTGGCTACATGA
PRIMER_INTERNAL_2_SEQUENCE=CGCCATTCCACAAATAACCT
PRIMER_LEFT_2=8,20
PRIMER_RIGHT_2=96,20
PRIMER_INTERNAL_2=57,20
PRIMER_LEFT_2_TM=59.405
PRIMER_RIGHT_2_TM=59.241
PRIMER_INTERNAL_2_TM=59.823
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=1.451852
PRIMER_LEFT_3_PENALTY=0.759154
PRIMER_RIGHT_3_PENALTY=0.692698
PRIMER_INTERNAL_3_PENALTY=3.156444
PRIMER_LEFT_3_SEQUENCE=TCATGTAGCCAACTCCAGGA
PRIMER_RIGHT_3_SEQUENCE=ATCCCAGGGCACTGAAATTT
PRIMER_INTERNAL_3_SEQUENCE=GGATTTCATCCTTTTTTCAACC
PRIMER_LEFT_3=77,20
PRIMER_RIGHT_3=171,20
PRIMER_INTERNAL_3=125,22
PRIMER_LEFT_3_TM=59.241
PRIMER_RIGHT_3_TM=60.693
PRIMER_INTERNAL_3_TM=58.844
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=36.364
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=1.592521
PRIMER_LEFT_4_PENALTY=1.239452
PRIMER_RIGHT_4_PENALTY=0.353069
PRIMER_INTERNAL_4_PENALTY=0.648069
PRIMER_LEFT_4_SEQUENCE=ATGCTGTGGCTTTTTAGACCA
PRIMER_RIGHT_4_SEQUENCE=AAACATTCCAGTGGGGATTG
PRIMER_INTERNAL_4_SEQUENCE=TCTCCTCTGAGGTCCAGTCC
PRIMER_LEFT_4=205,21
PRIMER_RIGHT_4=302,20
PRIMER_INTERNAL_4=262,20
PRIMER_LEFT_4_TM=59.761
PRIMER_RIGHT_4_TM=59.647
PRIMER_INTERNAL_4_TM=59.352
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH203
SEQUENCE_TEMPLATE=TGAATAACCAATCCACTCCGCTTTTAGTNGGAGTTTTAGTTGGTTAGTTTTGTCAAACCCACAATAAAACCCTGGGGTGGACCAAGAGAGTNNGCCACTCTAGTCCTTGGCTTTCTATATAGCAGGAGGTGCAGACCTTGGAATCTGGAGGAGTCTCTGGTGGCCCTCCCTTGGCCTTTAAATTTCACCCCACCCTCTCCCTCTCCTGAGGCTTTCTGGGCCTCATCTCTGTGCTGAATGCAAAAATGACCACACTTGGCATTCAAGACTCTTCATGACTTCGCTCCTGCTTACTTCTCCAGGGTAC
SEQUENCE_INCLUDED_REGION=0,307
P3_COMMENT=CL is TGAATAACCAATCCACTCCG
P3_COMMENT=CR is ACCCTGGAGAAGTAAGCAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.093581
PRIMER_LEFT_0_PENALTY=0.081111
PRIMER_RIGHT_0_PENALTY=0.012470
PRIMER_INTERNAL_0_PENALTY=1.100593
PRIMER_LEFT_0_SEQUENCE=CACAATAAAACCCTGGGGTG
PRIMER_RIGHT_0_SEQUENCE=AAGGTCTGCACCTCCTGCTA
PRIMER_INTERNAL_0_SEQUENCE=GCCACTCTAGTCCTTGGCTTT
PRIMER_LEFT_0=59,20
PRIMER_RIGHT_0=138,20
PRIMER_INTERNAL_0=93,21
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=60.012
PRIMER_INTERNAL_0_TM=59.899
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=0.093963
PRIMER_LEFT_1_PENALTY=0.081493
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_INTERNAL_1_PENALTY=1.100593
PRIMER_LEFT_1_SEQUENCE=AATAAAACCCTGGGGTGGAC
PRIMER_RIGHT_1_SEQUENCE=AAGGTCTGCACCTCCTGCTA
PRIMER_INTERNAL_1_SEQUENCE=GCCACTCTAGTCCTTGGCTTT
PRIMER_LEFT_1=62,20
PRIMER_RIGHT_1=138,20
PRIMER_INTERNAL_1=93,21
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=60.012
PRIMER_INTERNAL_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=0.100814
PRIMER_LEFT_2_PENALTY=0.012470
PRIMER_RIGHT_2_PENALTY=0.088344
PRIMER_INTERNAL_2_PENALTY=0.387849
PRIMER_LEFT_2_SEQUENCE=TAGCAGGAGGTGCAGACCTT
PRIMER_RIGHT_2_SEQUENCE=AAGCCTCAGGAGAGGGAGAG
PRIMER_INTERNAL_2_SEQUENCE=TCTGGAGGAGTCTCTGGTGG
PRIMER_LEFT_2=119,20
PRIMER_RIGHT_2=213,20
PRIMER_INTERNAL_2=143,20
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.088
PRIMER_INTERNAL_2_TM=60.388
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=0.181120
PRIMER_LEFT_3_PENALTY=0.012470
PRIMER_RIGHT_3_PENALTY=0.168650
PRIMER_INTERNAL_3_PENALTY=0.387849
PRIMER_LEFT_3_SEQUENCE=TAGCAGGAGGTGCAGACCTT
PRIMER_RIGHT_3_SEQUENCE=GAGAGGGTGGGGTGAAATTT
PRIMER_INTERNAL_3_SEQUENCE=TCTGGAGGAGTCTCTGGTGG
PRIMER_LEFT_3=119,20
PRIMER_RIGHT_3=198,20
PRIMER_INTERNAL_3=143,20
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=60.169
PRIMER_INTERNAL_3_TM=60.388
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=0.201595
PRIMER_LEFT_4_PENALTY=0.168650
PRIMER_RIGHT_4_PENALTY=0.032945
PRIMER_INTERNAL_4_PENALTY=0.056936
PRIMER_LEFT_4_SEQUENCE=AAATTTCACCCCACCCTCTC
PRIMER_RIGHT_4_SEQUENCE=TGAATGCCAAGTGTGGTCAT
PRIMER_INTERNAL_4_SEQUENCE=CTTTCTGGGCCTCATCTCTG
PRIMER_LEFT_4=179,20
PRIMER_RIGHT_4=264,20
PRIMER_INTERNAL_4=211,20
PRIMER_LEFT_4_TM=60.169
PRIMER_RIGHT_4_TM=59.967
PRIMER_INTERNAL_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH209
SEQUENCE_TEMPLATE=CTAGCACCATAGGAAGCCACTAATTATATGCGACCAAAGTGATGAACATAGCAAATAGGTGGTACCTGTGGCACATTTTAGGCATTTTAAAATAAATCTTGTGGCAATGCGTTGAAGGTTTGAATGCAAATGGATCAATTCAATATACTTNTATCTCTACAGGATAGTGGCACTTGACAGTTGATAGAAAGGTAGAAAAATTAAAATGATCATCTCTTCCATGGGCCTTCTTCTATCCCTAAGTNAAATTGATTACTTCCTGGCCACCACTGCTTTATCTTTGCGTTTATTATATTTCAGTGACCACATTATATGAT
SEQUENCE_INCLUDED_REGION=0,317
P3_COMMENT=CL is CATAGGAAGCCACTAATTATATGCG
P3_COMMENT=CR is GTGGTCACTGAAATATAATAAACGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.094290
PRIMER_LEFT_0_PENALTY=0.024663
PRIMER_RIGHT_0_PENALTY=1.069627
PRIMER_INTERNAL_0_PENALTY=0.510724
PRIMER_LEFT_0_SEQUENCE=TAGGTGGTACCTGTGGCACA
PRIMER_RIGHT_0_SEQUENCE=TCCATTTGCATTCAAACCTTC
PRIMER_INTERNAL_0_SEQUENCE=AAATCTTGTGGCAATGCGTT
PRIMER_LEFT_0=55,20
PRIMER_RIGHT_0=133,21
PRIMER_INTERNAL_0=93,20
PRIMER_LEFT_0_TM=60.025
PRIMER_RIGHT_0_TM=59.930
PRIMER_INTERNAL_0_TM=60.511
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=79
PRIMER_PAIR_1_PENALTY=1.376295
PRIMER_LEFT_1_PENALTY=0.306669
PRIMER_RIGHT_1_PENALTY=1.069627
PRIMER_INTERNAL_1_PENALTY=0.510724
PRIMER_LEFT_1_SEQUENCE=AGGTGGTACCTGTGGCACAT
PRIMER_RIGHT_1_SEQUENCE=TCCATTTGCATTCAAACCTTC
PRIMER_INTERNAL_1_SEQUENCE=AAATCTTGTGGCAATGCGTT
PRIMER_LEFT_1=56,20
PRIMER_RIGHT_1=133,21
PRIMER_INTERNAL_1=93,20
PRIMER_LEFT_1_TM=60.307
PRIMER_RIGHT_1_TM=59.930
PRIMER_INTERNAL_1_TM=60.511
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=1.571022
PRIMER_LEFT_2_PENALTY=1.276733
PRIMER_RIGHT_2_PENALTY=0.294290
PRIMER_INTERNAL_2_PENALTY=0.000037
PRIMER_LEFT_2_SEQUENCE=TGCGACCAAAGTGATGAACA
PRIMER_RIGHT_2_SEQUENCE=TTCAAACCTTCAACGCATTG
PRIMER_INTERNAL_2_SEQUENCE=ACCTGTGGCACATTTTAGGC
PRIMER_LEFT_2=28,20
PRIMER_RIGHT_2=123,20
PRIMER_INTERNAL_2=63,20
PRIMER_LEFT_2_TM=61.277
PRIMER_RIGHT_2_TM=59.706
PRIMER_INTERNAL_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=1.600009
PRIMER_LEFT_3_PENALTY=1.305719
PRIMER_RIGHT_3_PENALTY=0.294290
PRIMER_INTERNAL_3_PENALTY=5.545167
PRIMER_LEFT_3_SEQUENCE=ATAGGTGGTACCTGTGGCACA
PRIMER_RIGHT_3_SEQUENCE=TTGCATTCAAACCTTCAACG
PRIMER_INTERNAL_3_SEQUENCE=GCATTTTAAAATAAATCTTGTGGCA
PRIMER_LEFT_3=54,21
PRIMER_RIGHT_3=128,20
PRIMER_INTERNAL_3=81,25
PRIMER_LEFT_3_TM=60.306
PRIMER_RIGHT_3_TM=59.706
PRIMER_INTERNAL_3_TM=60.545
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=28.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=7.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=1.621628
PRIMER_LEFT_4_PENALTY=0.036956
PRIMER_RIGHT_4_PENALTY=1.584672
PRIMER_INTERNAL_4_PENALTY=2.431064
PRIMER_LEFT_4_SEQUENCE=ATCTCTTCCATGGGCCTTCT
PRIMER_RIGHT_4_SEQUENCE=AACGCAAAGATAAAGCAGTGG
PRIMER_INTERNAL_4_SEQUENCE=AAATTGATTACTTCCTGGCCA
PRIMER_LEFT_4=211,20
PRIMER_RIGHT_4=286,21
PRIMER_INTERNAL_4=245,21
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=59.415
PRIMER_INTERNAL_4_TM=58.569
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH215
SEQUENCE_TEMPLATE=CTCCTGCCCTCCGACGGCGTCTCCAGGCCCCAAACGGCCTCCGGTCGGTGGGCTTCTCCAGGCCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
SEQUENCE_INCLUDED_REGION=0,66
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH223
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTTACTACTCTCATATCTATTCANTCCCATCAGTACATAAGAAACTAGGGAAAGAAACATTNTGGTTTGAATCTGTTCTTAAGTAGGNACAATACACATGACTAATTTAAAATATTTTCCAANGTTCTTACACCCGTNATATCTAAATTTGNTACCATCANCTAAAGATTGAAGGCACTGNATTTAAAAAAAAAA
SEQUENCE_INCLUDED_REGION=35,199
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH224
SEQUENCE_TEMPLATE=CTAGTTTTGTNATGTATTATTTATTTATTTATTTCTATATTTATTGCAGAGATGAGGTATTACTCTGTTGCCCAGGCTGGTCTCAGACTCCTCAGTGCAAGTNAATCCTCCTCCCTTGGCCTCCCCAAATGCTGGGATTACAGAGGTGAGACATCATGCCTGGCCATGGTTGCTGGTTTTGAAGATGTGGGAAGGGGACCATGAGCCAAGGAGTGCAGGTGGCCTGTAGAATCTGGGAAAGGAAAGGAAGTGGATTCCCCCAGAG
SEQUENCE_INCLUDED_REGION=0,265
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.510386
PRIMER_LEFT_0_PENALTY=0.096855
PRIMER_RIGHT_0_PENALTY=0.413531
PRIMER_INTERNAL_0_PENALTY=0.141511
PRIMER_LEFT_0_SEQUENCE=TTTTGAAGATGTGGGAAGGG
PRIMER_RIGHT_0_SEQUENCE=TCCACTTCCTTTCCTTTCCC
PRIMER_INTERNAL_0_SEQUENCE=GTGCAGGTGGCCTGTAGAAT
PRIMER_LEFT_0=176,20
PRIMER_RIGHT_0=253,20
PRIMER_INTERNAL_0=212,20
PRIMER_LEFT_0_TM=59.903
PRIMER_RIGHT_0_TM=60.414
PRIMER_INTERNAL_0_TM=60.142
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=0.722253
PRIMER_LEFT_1_PENALTY=0.308722
PRIMER_RIGHT_1_PENALTY=0.413531
PRIMER_INTERNAL_1_PENALTY=0.141511
PRIMER_LEFT_1_SEQUENCE=TGCTGGTTTTGAAGATGTGG
PRIMER_RIGHT_1_SEQUENCE=TCCACTTCCTTTCCTTTCCC
PRIMER_INTERNAL_1_SEQUENCE=GTGCAGGTGGCCTGTAGAAT
PRIMER_LEFT_1=170,20
PRIMER_RIGHT_1=253,20
PRIMER_INTERNAL_1=212,20
PRIMER_LEFT_1_TM=59.691
PRIMER_RIGHT_1_TM=60.414
PRIMER_INTERNAL_1_TM=60.142
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=0.723698
PRIMER_LEFT_2_PENALTY=0.096855
PRIMER_RIGHT_2_PENALTY=0.626843
PRIMER_INTERNAL_2_PENALTY=0.072951
PRIMER_LEFT_2_SEQUENCE=TTTTGAAGATGTGGGAAGGG
PRIMER_RIGHT_2_SEQUENCE=GGGAATCCACTTCCTTTCCT
PRIMER_INTERNAL_2_SEQUENCE=GTGGCCTGTAGAATCTGGGA
PRIMER_LEFT_2=176,20
PRIMER_RIGHT_2=258,20
PRIMER_INTERNAL_2=218,20
PRIMER_LEFT_2_TM=59.903
PRIMER_RIGHT_2_TM=59.373
PRIMER_INTERNAL_2_TM=60.073
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.861343
PRIMER_LEFT_3_PENALTY=0.788392
PRIMER_RIGHT_3_PENALTY=0.072951
PRIMER_INTERNAL_3_PENALTY=0.096855
PRIMER_LEFT_3_SEQUENCE=GAGGTGAGACATCATGCCTG
PRIMER_RIGHT_3_SEQUENCE=TCCCAGATTCTACAGGCCAC
PRIMER_INTERNAL_3_SEQUENCE=TTTTGAAGATGTGGGAAGGG
PRIMER_LEFT_3=142,20
PRIMER_RIGHT_3=237,20
PRIMER_INTERNAL_3=176,20
PRIMER_LEFT_3_TM=59.212
PRIMER_RIGHT_3_TM=60.073
PRIMER_INTERNAL_3_TM=59.903
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=0.861343
PRIMER_LEFT_4_PENALTY=0.788392
PRIMER_RIGHT_4_PENALTY=0.072951
PRIMER_INTERNAL_4_PENALTY=0.096855
PRIMER_LEFT_4_SEQUENCE=CAGAGGTGAGACATCATGCC
PRIMER_RIGHT_4_SEQUENCE=TCCCAGATTCTACAGGCCAC
PRIMER_INTERNAL_4_SEQUENCE=TTTTGAAGATGTGGGAAGGG
PRIMER_LEFT_4=140,20
PRIMER_RIGHT_4=237,20
PRIMER_INTERNAL_4=176,20
PRIMER_LEFT_4_TM=59.212
PRIMER_RIGHT_4_TM=60.073
PRIMER_INTERNAL_4_TM=59.903
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH227
SEQUENCE_TEMPLATE=CTTTTGGTNNTTAAGCCCTGGGTNCTTTCACCTCCACAAAGATGCTTCACAGAGGTGTAAAGTGTNTTAGTCTCAATGGTCTTATACGGCGTTCAATCACATGAGACTCAAGAGGAGCAGTCACCTCAGTAATCTTAATTTTCTCTGTGCTTTCCTTCTGCTAGAAATCTTGGCCCTCCATCATAGAATTATCTGGAAAAGCCCAGGGGAGTTGAAATATGACTCAGTGCAATTCTATTTTTAGACACAGACTTAATGGTTGACTCCCATTGAAATAACTGAAATACAG
SEQUENCE_INCLUDED_REGION=0,289
P3_COMMENT=CL is TCAATGGTCTTATACGGCGT
P3_COMMENT=CR is TTCAATGGGAGTCAACCATT
P3_COMMENT=CM is CAATCACATGAGACTCAAGAGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.748631
PRIMER_LEFT_0_PENALTY=0.575240
PRIMER_RIGHT_0_PENALTY=0.173391
PRIMER_INTERNAL_0_PENALTY=0.875462
PRIMER_LEFT_0_SEQUENCE=ATACGGCGTTCAATCACATG
PRIMER_RIGHT_0_SEQUENCE=GGAGGGCCAAGATTTCTAGC
PRIMER_INTERNAL_0_SEQUENCE=CAAGAGGAGCAGTCACCTCA
PRIMER_LEFT_0=83,20
PRIMER_RIGHT_0=178,20
PRIMER_INTERNAL_0=108,20
PRIMER_LEFT_0_TM=59.425
PRIMER_RIGHT_0_TM=60.173
PRIMER_INTERNAL_0_TM=59.125
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=0.904425
PRIMER_LEFT_1_PENALTY=0.875462
PRIMER_RIGHT_1_PENALTY=0.028963
PRIMER_INTERNAL_1_PENALTY=1.251196
PRIMER_LEFT_1_SEQUENCE=CAAGAGGAGCAGTCACCTCA
PRIMER_RIGHT_1_SEQUENCE=TTCTATGATGGAGGGCCAAG
PRIMER_INTERNAL_1_SEQUENCE=TTCTCTGTGCTTTCCTTCTGC
PRIMER_LEFT_1=108,20
PRIMER_RIGHT_1=187,20
PRIMER_INTERNAL_1=140,21
PRIMER_LEFT_1_TM=59.125
PRIMER_RIGHT_1_TM=60.029
PRIMER_INTERNAL_1_TM=59.749
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=0.984751
PRIMER_LEFT_2_PENALTY=0.575240
PRIMER_RIGHT_2_PENALTY=0.409511
PRIMER_INTERNAL_2_PENALTY=0.875462
PRIMER_LEFT_2_SEQUENCE=ATACGGCGTTCAATCACATG
PRIMER_RIGHT_2_SEQUENCE=GATGGAGGGCCAAGATTTCT
PRIMER_INTERNAL_2_SEQUENCE=CAAGAGGAGCAGTCACCTCA
PRIMER_LEFT_2=83,20
PRIMER_RIGHT_2=181,20
PRIMER_INTERNAL_2=108,20
PRIMER_LEFT_2_TM=59.425
PRIMER_RIGHT_2_TM=60.410
PRIMER_INTERNAL_2_TM=59.125
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=1.079907
PRIMER_LEFT_3_PENALTY=0.906516
PRIMER_RIGHT_3_PENALTY=0.173391
PRIMER_INTERNAL_3_PENALTY=0.875462
PRIMER_LEFT_3_SEQUENCE=GGCGTTCAATCACATGAGAC
PRIMER_RIGHT_3_SEQUENCE=GGAGGGCCAAGATTTCTAGC
PRIMER_INTERNAL_3_SEQUENCE=CAAGAGGAGCAGTCACCTCA
PRIMER_LEFT_3=87,20
PRIMER_RIGHT_3=178,20
PRIMER_INTERNAL_3=108,20
PRIMER_LEFT_3_TM=59.093
PRIMER_RIGHT_3_TM=60.173
PRIMER_INTERNAL_3_TM=59.125
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=1.164835
PRIMER_LEFT_4_PENALTY=0.575240
PRIMER_RIGHT_4_PENALTY=0.589594
PRIMER_INTERNAL_4_PENALTY=0.875462
PRIMER_LEFT_4_SEQUENCE=ATACGGCGTTCAATCACATG
PRIMER_RIGHT_4_SEQUENCE=GGGCCAAGATTTCTAGCAGA
PRIMER_INTERNAL_4_SEQUENCE=CAAGAGGAGCAGTCACCTCA
PRIMER_LEFT_4=83,20
PRIMER_RIGHT_4=175,20
PRIMER_INTERNAL_4=108,20
PRIMER_LEFT_4_TM=59.425
PRIMER_RIGHT_4_TM=59.410
PRIMER_INTERNAL_4_TM=59.125
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH229
SEQUENCE_TEMPLATE=CTAAATATTCCAGGTCAGTGAAAATGCAAAGACATTTTAGGTCACAGGAAACCACCTGGGATGTTTCAGCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCA
SEQUENCE_INCLUDED_REGION=0,72
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH232
SEQUENCE_TEMPLATE=CTACTAAGCAAATTCTGCTCAGCCTTCTGCCTTTCCTGACTGTCTTGTTGGCCCTTCCCACTTTAAGGATGCCTGTTTAAGTAGCCACCTCTAATTAGGAATCTTCCCTTGTTCTTTCTCAGGAGGCTTAGACACTGTCAGTTTCCTGAAGACAGAAAATAAGCCTGCATTATCCTAGTAGTGGATTCAAAACTAATTGTGTCCTGAGTCTTTCAATCATCAAGACATGGGAAACACTCAACAG
SEQUENCE_INCLUDED_REGION=0,244
P3_COMMENT=CL is TTCTGCCTTTCCTGACTGTC
P3_COMMENT=CR is TGTTTCCCATGTCTTGATGA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.521276
PRIMER_LEFT_0_PENALTY=0.296722
PRIMER_RIGHT_0_PENALTY=1.224554
PRIMER_INTERNAL_0_PENALTY=1.761718
PRIMER_LEFT_0_SEQUENCE=GACTGTCTTGTTGGCCCTTC
PRIMER_RIGHT_0_SEQUENCE=CCTCCTGAGAAAGAACAAGGG
PRIMER_INTERNAL_0_SEQUENCE=GGATGCCTGTTTAAGTAGCCA
PRIMER_LEFT_0=37,20
PRIMER_RIGHT_0=125,21
PRIMER_INTERNAL_0=66,21
PRIMER_LEFT_0_TM=59.703
PRIMER_RIGHT_0_TM=60.225
PRIMER_INTERNAL_0_TM=59.238
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=1.522572
PRIMER_LEFT_1_PENALTY=0.298018
PRIMER_RIGHT_1_PENALTY=1.224554
PRIMER_INTERNAL_1_PENALTY=0.066323
PRIMER_LEFT_1_SEQUENCE=CTGACTGTCTTGTTGGCCCT
PRIMER_RIGHT_1_SEQUENCE=CCTCCTGAGAAAGAACAAGGG
PRIMER_INTERNAL_1_SEQUENCE=TCCCACTTTAAGGATGCCTG
PRIMER_LEFT_1=35,20
PRIMER_RIGHT_1=125,21
PRIMER_INTERNAL_1=55,20
PRIMER_LEFT_1_TM=60.298
PRIMER_RIGHT_1_TM=60.225
PRIMER_INTERNAL_1_TM=60.066
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=1.664438
PRIMER_LEFT_2_PENALTY=0.296722
PRIMER_RIGHT_2_PENALTY=1.367716
PRIMER_INTERNAL_2_PENALTY=1.761718
PRIMER_LEFT_2_SEQUENCE=GACTGTCTTGTTGGCCCTTC
PRIMER_RIGHT_2_SEQUENCE=GCCTCCTGAGAAAGAACAAGG
PRIMER_INTERNAL_2_SEQUENCE=GGATGCCTGTTTAAGTAGCCA
PRIMER_LEFT_2=37,20
PRIMER_RIGHT_2=126,21
PRIMER_INTERNAL_2=66,21
PRIMER_LEFT_2_TM=59.703
PRIMER_RIGHT_2_TM=60.368
PRIMER_INTERNAL_2_TM=59.238
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=1.665734
PRIMER_LEFT_3_PENALTY=0.298018
PRIMER_RIGHT_3_PENALTY=1.367716
PRIMER_INTERNAL_3_PENALTY=0.066323
PRIMER_LEFT_3_SEQUENCE=CTGACTGTCTTGTTGGCCCT
PRIMER_RIGHT_3_SEQUENCE=GCCTCCTGAGAAAGAACAAGG
PRIMER_INTERNAL_3_SEQUENCE=TCCCACTTTAAGGATGCCTG
PRIMER_LEFT_3=35,20
PRIMER_RIGHT_3=126,21
PRIMER_INTERNAL_3=55,20
PRIMER_LEFT_3_TM=60.298
PRIMER_RIGHT_3_TM=60.368
PRIMER_INTERNAL_3_TM=60.066
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=1.804742
PRIMER_LEFT_4_PENALTY=0.580189
PRIMER_RIGHT_4_PENALTY=1.224554
PRIMER_INTERNAL_4_PENALTY=2.978554
PRIMER_LEFT_4_SEQUENCE=GCCCTTCCCACTTTAAGGAT
PRIMER_RIGHT_4_SEQUENCE=CCTCCTGAGAAAGAACAAGGG
PRIMER_INTERNAL_4_SEQUENCE=GCCTGTTTAAGTAGCCACCTC
PRIMER_LEFT_4=50,20
PRIMER_RIGHT_4=125,21
PRIMER_INTERNAL_4=70,21
PRIMER_LEFT_4_TM=59.420
PRIMER_RIGHT_4_TM=60.225
PRIMER_INTERNAL_4_TM=58.021
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH233
SEQUENCE_TEMPLATE=CTTATTTAAATAAATAATGTCTGAAAACTTCCCAAATCTTGGTAGGAATATGGCTATCCATAATCATGAGGCTCAAGCAACATTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGNGAAATNGTTATCCGCTNACANTTCCACANAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTANTGAGTNAGCTAACTCNCATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCA
SEQUENCE_INCLUDED_REGION=0,86
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.722379
PRIMER_LEFT_0_PENALTY=5.626841
PRIMER_RIGHT_0_PENALTY=2.095538
PRIMER_INTERNAL_0_PENALTY=3.527447
PRIMER_LEFT_0_SEQUENCE=AAATAATGTCTGAAAACTTCCCAAA
PRIMER_RIGHT_0_SEQUENCE=CTAATGTTGCTTGAGCCTCATG
PRIMER_INTERNAL_0_SEQUENCE=TTGGTAGGAATATGGCTATCCA
PRIMER_LEFT_0=11,25
PRIMER_RIGHT_0=85,22
PRIMER_INTERNAL_0=38,22
PRIMER_LEFT_0_TM=59.373
PRIMER_RIGHT_0_TM=59.904
PRIMER_INTERNAL_0_TM=58.473
PRIMER_LEFT_0_GC_PERCENT=28.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=7.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=7.895837
PRIMER_LEFT_1_PENALTY=5.800299
PRIMER_RIGHT_1_PENALTY=2.095538
PRIMER_INTERNAL_1_PENALTY=3.527447
PRIMER_LEFT_1_SEQUENCE=AAATAATGTCTGAAAACTTCCCAA
PRIMER_RIGHT_1_SEQUENCE=CTAATGTTGCTTGAGCCTCATG
PRIMER_INTERNAL_1_SEQUENCE=TTGGTAGGAATATGGCTATCCA
PRIMER_LEFT_1=11,24
PRIMER_RIGHT_1=85,22
PRIMER_INTERNAL_1=38,22
PRIMER_LEFT_1_TM=58.200
PRIMER_RIGHT_1_TM=59.904
PRIMER_INTERNAL_1_TM=58.473
PRIMER_LEFT_1_GC_PERCENT=29.167
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=8.735608
PRIMER_LEFT_2_PENALTY=7.612130
PRIMER_RIGHT_2_PENALTY=1.123478
PRIMER_INTERNAL_2_PENALTY=3.527447
PRIMER_LEFT_2_SEQUENCE=ATAAATAATGTCTGAAAACTTCCCA
PRIMER_RIGHT_2_SEQUENCE=AATGTTGCTTGAGCCTCATG
PRIMER_INTERNAL_2_SEQUENCE=TTGGTAGGAATATGGCTATCCA
PRIMER_LEFT_2=9,25
PRIMER_RIGHT_2=83,20
PRIMER_INTERNAL_2=38,22
PRIMER_LEFT_2_TM=57.388
PRIMER_RIGHT_2_TM=58.877
PRIMER_INTERNAL_2_TM=58.473
PRIMER_LEFT_2_GC_PERCENT=28.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=7.00
PRIMER_INTERNAL_2_SELF_END=8.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=8.802618
PRIMER_LEFT_3_PENALTY=6.733780
PRIMER_RIGHT_3_PENALTY=2.068838
PRIMER_INTERNAL_3_PENALTY=3.527447
PRIMER_LEFT_3_SEQUENCE=TAAATAATGTCTGAAAACTTCCCAA
PRIMER_RIGHT_3_SEQUENCE=TAATGTTGCTTGAGCCTCATG
PRIMER_INTERNAL_3_SEQUENCE=TTGGTAGGAATATGGCTATCCA
PRIMER_LEFT_3=10,25
PRIMER_RIGHT_3=84,21
PRIMER_INTERNAL_3=38,22
PRIMER_LEFT_3_TM=58.266
PRIMER_RIGHT_3_TM=58.931
PRIMER_INTERNAL_3_TM=58.473
PRIMER_LEFT_3_GC_PERCENT=28.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=7.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=8.829318
PRIMER_LEFT_4_PENALTY=6.733780
PRIMER_RIGHT_4_PENALTY=2.095538
PRIMER_INTERNAL_4_PENALTY=3.527447
PRIMER_LEFT_4_SEQUENCE=TAAATAATGTCTGAAAACTTCCCAA
PRIMER_RIGHT_4_SEQUENCE=CTAATGTTGCTTGAGCCTCATG
PRIMER_INTERNAL_4_SEQUENCE=TTGGTAGGAATATGGCTATCCA
PRIMER_LEFT_4=10,25
PRIMER_RIGHT_4=85,22
PRIMER_INTERNAL_4=38,22
PRIMER_LEFT_4_TM=58.266
PRIMER_RIGHT_4_TM=59.904
PRIMER_INTERNAL_4_TM=58.473
PRIMER_LEFT_4_GC_PERCENT=28.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=7.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH238
SEQUENCE_TEMPLATE=AAAGACTACCCATAGTATGAGTCAATTTATTTTAAAATTACAGAAGATGCTAAACTAGGAGCAGCAAGGAGATTAGTACTTTCAGAGATCAGGGCAAGGGAAGGGGCTTGACTGCAAATGGACACAAGAAAACTTTTTAAGTAACGAATATGTTCTGTATCATGATTCTGATCATGGTTTAANTACCTGTCAAAATTCATCAATTATACATTTTAAATTGGTAAACTTTATTCCAAGTAATTCATACCT
SEQUENCE_INCLUDED_REGION=0,249
P3_COMMENT=CL is AAGATGCTAAACTAGGAGCAGC
P3_COMMENT=CR is GAATTACTTGGAATAAAGTTTACCA
P3_COMMENT=CM is GATTAGTACTTTCAGAGATCAGGGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.871921
PRIMER_LEFT_0_PENALTY=2.169174
PRIMER_RIGHT_0_PENALTY=0.702747
PRIMER_INTERNAL_0_PENALTY=0.331043
PRIMER_LEFT_0_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
PRIMER_RIGHT_0_SEQUENCE=GTGTCCATTTGCAGTCAAGC
PRIMER_INTERNAL_0_SEQUENCE=TTTCAGAGATCAGGGCAAGG
PRIMER_LEFT_0=48,22
PRIMER_RIGHT_0=124,20
PRIMER_INTERNAL_0=79,20
PRIMER_LEFT_0_TM=60.169
PRIMER_RIGHT_0_TM=59.297
PRIMER_INTERNAL_0_TM=60.331
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=3.263418
PRIMER_LEFT_1_PENALTY=2.560670
PRIMER_RIGHT_1_PENALTY=0.702747
PRIMER_INTERNAL_1_PENALTY=0.331043
PRIMER_LEFT_1_SEQUENCE=GCTAAACTAGGAGCAGCAAGG
PRIMER_RIGHT_1_SEQUENCE=GTGTCCATTTGCAGTCAAGC
PRIMER_INTERNAL_1_SEQUENCE=TTTCAGAGATCAGGGCAAGG
PRIMER_LEFT_1=48,21
PRIMER_RIGHT_1=124,20
PRIMER_INTERNAL_1=79,20
PRIMER_LEFT_1_TM=58.439
PRIMER_RIGHT_1_TM=59.297
PRIMER_INTERNAL_1_TM=60.331
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=3.420435
PRIMER_LEFT_2_PENALTY=2.169174
PRIMER_RIGHT_2_PENALTY=1.251261
PRIMER_INTERNAL_2_PENALTY=0.331043
PRIMER_LEFT_2_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
PRIMER_RIGHT_2_SEQUENCE=TTGTGTCCATTTGCAGTCAAG
PRIMER_INTERNAL_2_SEQUENCE=TTTCAGAGATCAGGGCAAGG
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=126,21
PRIMER_INTERNAL_2=79,20
PRIMER_LEFT_2_TM=60.169
PRIMER_RIGHT_2_TM=59.749
PRIMER_INTERNAL_2_TM=60.331
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=3.420435
PRIMER_LEFT_3_PENALTY=2.169174
PRIMER_RIGHT_3_PENALTY=1.251261
PRIMER_INTERNAL_3_PENALTY=0.331043
PRIMER_LEFT_3_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
PRIMER_RIGHT_3_SEQUENCE=CTTGTGTCCATTTGCAGTCAA
PRIMER_INTERNAL_3_SEQUENCE=TTTCAGAGATCAGGGCAAGG
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=127,21
PRIMER_INTERNAL_3=79,20
PRIMER_LEFT_3_TM=60.169
PRIMER_RIGHT_3_TM=59.749
PRIMER_INTERNAL_3_TM=60.331
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=3.463126
PRIMER_LEFT_4_PENALTY=2.169174
PRIMER_RIGHT_4_PENALTY=1.293952
PRIMER_INTERNAL_4_PENALTY=0.331043
PRIMER_LEFT_4_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA
PRIMER_RIGHT_4_SEQUENCE=TCTTGTGTCCATTTGCAGTCA
PRIMER_INTERNAL_4_SEQUENCE=TTTCAGAGATCAGGGCAAGG
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=128,21
PRIMER_INTERNAL_4=79,20
PRIMER_LEFT_4_TM=60.169
PRIMER_RIGHT_4_TM=60.294
PRIMER_INTERNAL_4_TM=60.331
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH240
SEQUENCE_TEMPLATE=CAGATCTGTAATCTGCTTTCTAGATCCCTTTCTTACCCATTCTTAAGCCATCACAGCAGAGTACCTTTCTAACTTATAAGATTGTGTAGAGGTTGGAAGGAGGACAGGACTGTTCTGTTGGTATAATGACCCTGTGTCCAGTTAATCCATG
SEQUENCE_INCLUDED_REGION=0,151
P3_COMMENT=CL is AGCCATCACAGCAGAGTACC
P3_COMMENT=CR is TGGATTAACTGGACACAGGG
P3_COMMENT=CM is GTGTAGAGGTTGGAAGGAGGA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.067153
PRIMER_LEFT_0_PENALTY=1.278288
PRIMER_RIGHT_0_PENALTY=1.788865
PRIMER_INTERNAL_0_PENALTY=4.295192
PRIMER_LEFT_0_SEQUENCE=CCATTCTTAAGCCATCACAGC
PRIMER_RIGHT_0_SEQUENCE=CCAACAGAACAGTCCTGTCCT
PRIMER_INTERNAL_0_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG
PRIMER_LEFT_0=36,21
PRIMER_RIGHT_0=120,21
PRIMER_INTERNAL_0=77,23
PRIMER_LEFT_0_TM=59.722
PRIMER_RIGHT_0_TM=59.211
PRIMER_INTERNAL_0_TM=58.705
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=9.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=8.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=3.165558
PRIMER_LEFT_1_PENALTY=1.278288
PRIMER_RIGHT_1_PENALTY=1.887270
PRIMER_INTERNAL_1_PENALTY=3.825817
PRIMER_LEFT_1_SEQUENCE=CCATTCTTAAGCCATCACAGC
PRIMER_RIGHT_1_SEQUENCE=CCAACAGAACAGTCCTGTCC
PRIMER_INTERNAL_1_SEQUENCE=GATTGTGTAGAGGTTGGAAGGA
PRIMER_LEFT_1=36,21
PRIMER_RIGHT_1=120,20
PRIMER_INTERNAL_1=79,22
PRIMER_LEFT_1_TM=59.722
PRIMER_RIGHT_1_TM=58.113
PRIMER_INTERNAL_1_TM=58.174
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=9.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=3.211098
PRIMER_LEFT_2_PENALTY=1.422232
PRIMER_RIGHT_2_PENALTY=1.788865
PRIMER_INTERNAL_2_PENALTY=4.295192
PRIMER_LEFT_2_SEQUENCE=CCCATTCTTAAGCCATCACAG
PRIMER_RIGHT_2_SEQUENCE=CCAACAGAACAGTCCTGTCCT
PRIMER_INTERNAL_2_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG
PRIMER_LEFT_2=35,21
PRIMER_RIGHT_2=120,21
PRIMER_INTERNAL_2=77,23
PRIMER_LEFT_2_TM=59.578
PRIMER_RIGHT_2_TM=59.211
PRIMER_INTERNAL_2_TM=58.705
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=9.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=8.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=3.214224
PRIMER_LEFT_3_PENALTY=1.278288
PRIMER_RIGHT_3_PENALTY=1.935937
PRIMER_INTERNAL_3_PENALTY=3.825817
PRIMER_LEFT_3_SEQUENCE=CCATTCTTAAGCCATCACAGC
PRIMER_RIGHT_3_SEQUENCE=ACCAACAGAACAGTCCTGTCC
PRIMER_INTERNAL_3_SEQUENCE=GATTGTGTAGAGGTTGGAAGGA
PRIMER_LEFT_3=36,21
PRIMER_RIGHT_3=121,21
PRIMER_INTERNAL_3=79,22
PRIMER_LEFT_3_TM=59.722
PRIMER_RIGHT_3_TM=59.064
PRIMER_INTERNAL_3_TM=58.174
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=9.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=3.216673
PRIMER_LEFT_4_PENALTY=1.427808
PRIMER_RIGHT_4_PENALTY=1.788865
PRIMER_INTERNAL_4_PENALTY=4.295192
PRIMER_LEFT_4_SEQUENCE=CCCATTCTTAAGCCATCACA
PRIMER_RIGHT_4_SEQUENCE=CCAACAGAACAGTCCTGTCCT
PRIMER_INTERNAL_4_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG
PRIMER_LEFT_4=35,20
PRIMER_RIGHT_4=120,21
PRIMER_INTERNAL_4=77,23
PRIMER_LEFT_4_TM=58.572
PRIMER_RIGHT_4_TM=59.211
PRIMER_INTERNAL_4_TM=58.705
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=9.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=8.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH243
SEQUENCE_TEMPLATE=NGGNNNCCNNCAGNGNCTGAAGGAAAGNACTGGCTTTTGGGGTACAAGGGTGACTAGAACCTTNCTGGGACTNTGGCTATAGTACTTTGAAATTATCACCCATATAAAAGAGGGACATGGACTATAACGTTGATTTCTTATTGCTGTACATTGCGTTTTTAACCTGCAAGTTCTCAGTTTCTTCACCATNACTCCATCAAAGNCCCTGGCTATAAGATCTGGATTTTACCCACTCCATCTTCTCTTTCCTTCTTACTGTGTNAATTCCTATTTTCTTTCAGAATCTTCTAAAAACAGTTGTATCTAACCGCATGTGCTGTTGTG
SEQUENCE_INCLUDED_REGION=0,324
P3_COMMENT=CL is TTGGGGTACAAGGGTGACTA
P3_COMMENT=CR is TGGAGTGGGTAAAATCCAGA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.271416
PRIMER_LEFT_0_PENALTY=1.059316
PRIMER_RIGHT_0_PENALTY=0.212099
PRIMER_INTERNAL_0_PENALTY=8.275230
PRIMER_LEFT_0_SEQUENCE=CCCACTCCATCTTCTCTTTCC
PRIMER_RIGHT_0_SEQUENCE=ACAACAGCACATGCGGTTAG
PRIMER_INTERNAL_0_SEQUENCE=TCTTTCAGAATCTTCTAAAAACAGTTG
PRIMER_LEFT_0=228,21
PRIMER_RIGHT_0=322,20
PRIMER_INTERNAL_0=273,27
PRIMER_LEFT_0_TM=60.059
PRIMER_RIGHT_0_TM=59.788
PRIMER_INTERNAL_0_TM=58.725
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=29.630
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=1.924735
PRIMER_LEFT_1_PENALTY=1.059316
PRIMER_RIGHT_1_PENALTY=0.865419
PRIMER_INTERNAL_1_PENALTY=8.275230
PRIMER_LEFT_1_SEQUENCE=CCCACTCCATCTTCTCTTTCC
PRIMER_RIGHT_1_SEQUENCE=CAACAGCACATGCGGTTAGA
PRIMER_INTERNAL_1_SEQUENCE=TCTTTCAGAATCTTCTAAAAACAGTTG
PRIMER_LEFT_1=228,21
PRIMER_RIGHT_1=321,20
PRIMER_INTERNAL_1=273,27
PRIMER_LEFT_1_TM=60.059
PRIMER_RIGHT_1_TM=60.865
PRIMER_INTERNAL_1_TM=58.725
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=29.630
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=2.056572
PRIMER_LEFT_2_PENALTY=0.997256
PRIMER_RIGHT_2_PENALTY=1.059316
PRIMER_INTERNAL_2_PENALTY=3.152909
PRIMER_LEFT_2_SEQUENCE=TTGCGTTTTTAACCTGCAAG
PRIMER_RIGHT_2_SEQUENCE=GGAAAGAGAAGATGGAGTGGG
PRIMER_INTERNAL_2_SEQUENCE=CCCTGGCTATAAGATCTGGATTT
PRIMER_LEFT_2=150,20
PRIMER_RIGHT_2=248,21
PRIMER_INTERNAL_2=203,23
PRIMER_LEFT_2_TM=59.003
PRIMER_RIGHT_2_TM=60.059
PRIMER_INTERNAL_2_TM=59.847
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=2.250975
PRIMER_LEFT_3_PENALTY=1.191659
PRIMER_RIGHT_3_PENALTY=1.059316
PRIMER_INTERNAL_3_PENALTY=3.152909
PRIMER_LEFT_3_SEQUENCE=TGCGTTTTTAACCTGCAAGTT
PRIMER_RIGHT_3_SEQUENCE=GGAAAGAGAAGATGGAGTGGG
PRIMER_INTERNAL_3_SEQUENCE=CCCTGGCTATAAGATCTGGATTT
PRIMER_LEFT_3=151,21
PRIMER_RIGHT_3=248,21
PRIMER_INTERNAL_3=203,23
PRIMER_LEFT_3_TM=59.808
PRIMER_RIGHT_3_TM=60.059
PRIMER_INTERNAL_3_TM=59.847
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=2.250975
PRIMER_LEFT_4_PENALTY=1.191659
PRIMER_RIGHT_4_PENALTY=1.059316
PRIMER_INTERNAL_4_PENALTY=3.152909
PRIMER_LEFT_4_SEQUENCE=TTGCGTTTTTAACCTGCAAGT
PRIMER_RIGHT_4_SEQUENCE=GGAAAGAGAAGATGGAGTGGG
PRIMER_INTERNAL_4_SEQUENCE=CCCTGGCTATAAGATCTGGATTT
PRIMER_LEFT_4=150,21
PRIMER_RIGHT_4=248,21
PRIMER_INTERNAL_4=203,23
PRIMER_LEFT_4_TM=59.808
PRIMER_RIGHT_4_TM=60.059
PRIMER_INTERNAL_4_TM=59.847
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH245
SEQUENCE_TEMPLATE=AAGCACAGTGGATATTTTTAGGCCTAGCATTCTTTATATTTATCCACTTAAAAATATATTAGTTTCACCTAGATCTAGGTAAAATAGTTTGCAAAAAGATTTTAGAATTGGGCAATTAATTTTAATTCTGACATCTTGGGCTTTTCACTTTTNCTTAGGCTCTCCTCGCTGACTTCGTTATTTACTTCATCTAGCATCCATCTTAACTTCTGTGTGAGCCCCTTGCCTTTGAAAACATGTCTTACTTTGT
SEQUENCE_INCLUDED_REGION=0,250
P3_COMMENT=CL is AGCACAGTGGATATTTTTAGGC
P3_COMMENT=CR is GGGGCTCACACAGAAGTTAA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.727342
PRIMER_LEFT_0_PENALTY=0.254998
PRIMER_RIGHT_0_PENALTY=0.472344
PRIMER_INTERNAL_0_PENALTY=4.219443
PRIMER_LEFT_0_SEQUENCE=CTCTCCTCGCTGACTTCGTT
PRIMER_RIGHT_0_SEQUENCE=CATGTTTTCAAAGGCAAGGG
PRIMER_INTERNAL_0_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
PRIMER_LEFT_0=159,20
PRIMER_RIGHT_0=238,20
PRIMER_INTERNAL_0=193,23
PRIMER_LEFT_0_TM=59.745
PRIMER_RIGHT_0_TM=60.472
PRIMER_INTERNAL_0_TM=58.781
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=1.139469
PRIMER_LEFT_1_PENALTY=0.667126
PRIMER_RIGHT_1_PENALTY=0.472344
PRIMER_INTERNAL_1_PENALTY=4.219443
PRIMER_LEFT_1_SEQUENCE=TAGGCTCTCCTCGCTGACTT
PRIMER_RIGHT_1_SEQUENCE=CATGTTTTCAAAGGCAAGGG
PRIMER_INTERNAL_1_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
PRIMER_LEFT_1=155,20
PRIMER_RIGHT_1=238,20
PRIMER_INTERNAL_1=193,23
PRIMER_LEFT_1_TM=59.333
PRIMER_RIGHT_1_TM=60.472
PRIMER_INTERNAL_1_TM=58.781
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=1.139469
PRIMER_LEFT_2_PENALTY=0.667126
PRIMER_RIGHT_2_PENALTY=0.472344
PRIMER_INTERNAL_2_PENALTY=4.219443
PRIMER_LEFT_2_SEQUENCE=TTAGGCTCTCCTCGCTGACT
PRIMER_RIGHT_2_SEQUENCE=CATGTTTTCAAAGGCAAGGG
PRIMER_INTERNAL_2_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
PRIMER_LEFT_2=154,20
PRIMER_RIGHT_2=238,20
PRIMER_INTERNAL_2=193,23
PRIMER_LEFT_2_TM=59.333
PRIMER_RIGHT_2_TM=60.472
PRIMER_INTERNAL_2_TM=58.781
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=1.139469
PRIMER_LEFT_3_PENALTY=0.667126
PRIMER_RIGHT_3_PENALTY=0.472344
PRIMER_INTERNAL_3_PENALTY=4.219443
PRIMER_LEFT_3_SEQUENCE=CTTAGGCTCTCCTCGCTGAC
PRIMER_RIGHT_3_SEQUENCE=CATGTTTTCAAAGGCAAGGG
PRIMER_INTERNAL_3_SEQUENCE=GCATCCATCTTAACTTCTGTGTG
PRIMER_LEFT_3=153,20
PRIMER_RIGHT_3=238,20
PRIMER_INTERNAL_3=193,23
PRIMER_LEFT_3_TM=59.333
PRIMER_RIGHT_3_TM=60.472
PRIMER_INTERNAL_3_TM=58.781
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=1.450582
PRIMER_LEFT_4_PENALTY=1.152564
PRIMER_RIGHT_4_PENALTY=0.298018
PRIMER_INTERNAL_4_PENALTY=0.254998
PRIMER_LEFT_4_SEQUENCE=CTGACATCTTGGGCTTTTCA
PRIMER_RIGHT_4_SEQUENCE=CAAGGGGCTCACACAGAAGT
PRIMER_INTERNAL_4_SEQUENCE=CTCTCCTCGCTGACTTCGTT
PRIMER_LEFT_4=127,20
PRIMER_RIGHT_4=224,20
PRIMER_INTERNAL_4=159,20
PRIMER_LEFT_4_TM=58.847
PRIMER_RIGHT_4_TM=60.298
PRIMER_INTERNAL_4_TM=59.745
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH250
SEQUENCE_TEMPLATE=TGAATCTAGTTCAGATAAAAAATGTGCTCAACCTCAGATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
SEQUENCE_INCLUDED_REGION=0,41
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH268
SEQUENCE_TEMPLATE=GCTCGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTTCATTTAATCAAAAGTTTAAAATTATAAGCATTAAAATGGCTGATAAATCACCTGCTAAAACCCTAGGAAAAAAATCTGCAACATGGAACAGGGACAGAAATCGTTAACTAAAGAGAAGAACTGGAAATTAAAAATGAGCATGAAATATGGTAGGATTAATAAANCAATAATAAGATAATCAGCAAGTTGGAAGTGAAATGGGATAATGTAGAAATTATGTTAGGACAGAAATTTACATCCTTATAACAG
SEQUENCE_INCLUDED_REGION=35,253
P3_COMMENT=CL is TGGCTGATAAATCACCTGCT
P3_COMMENT=CR is AGGATGTAAATTTCTGTCCTAACA
P3_COMMENT=CM is AAACCCTAGGAAAAAAATCTGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.532615
PRIMER_LEFT_0_PENALTY=0.480482
PRIMER_RIGHT_0_PENALTY=5.052133
PRIMER_INTERNAL_0_PENALTY=6.873727
PRIMER_LEFT_0_SEQUENCE=CAACATGGAACAGGGACAGA
PRIMER_RIGHT_0_SEQUENCE=TCCTACCATATTTCATGCTCATTT
PRIMER_INTERNAL_0_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA
PRIMER_LEFT_0=117,20
PRIMER_RIGHT_0=193,24
PRIMER_INTERNAL_0=139,26
PRIMER_LEFT_0_TM=59.520
PRIMER_RIGHT_0_TM=58.948
PRIMER_INTERNAL_0_TM=59.126
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=33.333
PRIMER_INTERNAL_0_GC_PERCENT=34.615
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=5.587782
PRIMER_LEFT_1_PENALTY=0.480482
PRIMER_RIGHT_1_PENALTY=5.107300
PRIMER_INTERNAL_1_PENALTY=6.873727
PRIMER_LEFT_1_SEQUENCE=CAACATGGAACAGGGACAGA
PRIMER_RIGHT_1_SEQUENCE=TCCTACCATATTTCATGCTCATTTT
PRIMER_INTERNAL_1_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA
PRIMER_LEFT_1=117,20
PRIMER_RIGHT_1=193,25
PRIMER_INTERNAL_1=139,26
PRIMER_LEFT_1_TM=59.520
PRIMER_RIGHT_1_TM=60.107
PRIMER_INTERNAL_1_TM=59.126
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=32.000
PRIMER_INTERNAL_1_GC_PERCENT=34.615
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=5.699135
PRIMER_LEFT_2_PENALTY=1.557510
PRIMER_RIGHT_2_PENALTY=4.141625
PRIMER_INTERNAL_2_PENALTY=4.708877
PRIMER_LEFT_2_SEQUENCE=GGAAAAAAATCTGCAACATGG
PRIMER_RIGHT_2_SEQUENCE=TTTCATGCTCATTTTTAATTTCCA
PRIMER_INTERNAL_2_SEQUENCE=AACAGGGACAGAAATCGTTAAC
PRIMER_LEFT_2=104,21
PRIMER_RIGHT_2=183,24
PRIMER_INTERNAL_2=125,22
PRIMER_LEFT_2_TM=59.442
PRIMER_RIGHT_2_TM=59.858
PRIMER_INTERNAL_2_TM=57.291
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=25.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=5.784504
PRIMER_LEFT_3_PENALTY=4.776618
PRIMER_RIGHT_3_PENALTY=1.007886
PRIMER_INTERNAL_3_PENALTY=2.627407
PRIMER_LEFT_3_SEQUENCE=GCATTAAAATGGCTGATAAATCA
PRIMER_RIGHT_3_SEQUENCE=ACGATTTCTGTCCCTGTTCC
PRIMER_INTERNAL_3_SEQUENCE=CCCTAGGAAAAAAATCTGCAA
PRIMER_LEFT_3=66,23
PRIMER_RIGHT_3=142,20
PRIMER_INTERNAL_3=99,21
PRIMER_LEFT_3_TM=58.223
PRIMER_RIGHT_3_TM=58.992
PRIMER_INTERNAL_3_TM=58.373
PRIMER_LEFT_3_GC_PERCENT=30.435
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=5.793391
PRIMER_LEFT_4_PENALTY=0.480482
PRIMER_RIGHT_4_PENALTY=5.312909
PRIMER_INTERNAL_4_PENALTY=6.873727
PRIMER_LEFT_4_SEQUENCE=CAACATGGAACAGGGACAGA
PRIMER_RIGHT_4_SEQUENCE=CCTACCATATTTCATGCTCATTTTT
PRIMER_INTERNAL_4_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA
PRIMER_LEFT_4=117,20
PRIMER_RIGHT_4=192,25
PRIMER_INTERNAL_4=139,26
PRIMER_LEFT_4_TM=59.520
PRIMER_RIGHT_4_TM=59.687
PRIMER_INTERNAL_4_TM=59.126
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=32.000
PRIMER_INTERNAL_4_GC_PERCENT=34.615
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH271
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTATTTTAAAAAAATTCTTATTTATTTATTTATTTATTTATTTATTTTGTAGAGANNAGGTCTTGTTACCTACCAGTCTGATCTTGAACTCCTGGCCACATGAAACTTATTAAAGGAAGTGACCATAATACTTTGTACAGTAGCATAGCACATTTATAATGCACATTGAACTTCAGGGGAATATATGAAAGTAAGAAGAAAATTTGCTTAGAACTTTCCTGCAGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCC
SEQUENCE_INCLUDED_REGION=35,224
P3_COMMENT=CL is AGGTCTTGTTACCTACCAGTCTG
P3_COMMENT=CR is TAAGCAAATTTTCTTCTTACTTTCA
P3_COMMENT=CM is TCTTGAACTCCTGGCCACAT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.041150
PRIMER_LEFT_0_PENALTY=0.026532
PRIMER_RIGHT_0_PENALTY=1.014618
PRIMER_INTERNAL_0_PENALTY=8.234100
PRIMER_LEFT_0_SEQUENCE=AACTCCTGGCCACATGAAAC
PRIMER_RIGHT_0_SEQUENCE=CCCTGAAGTTCAATGTGCATT
PRIMER_INTERNAL_0_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
PRIMER_LEFT_0=123,20
PRIMER_RIGHT_0=214,21
PRIMER_INTERNAL_0=166,26
PRIMER_LEFT_0_TM=59.973
PRIMER_RIGHT_0_TM=59.985
PRIMER_INTERNAL_0_TM=57.766
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=34.615
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=1.072499
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=1.045967
PRIMER_INTERNAL_1_PENALTY=8.234100
PRIMER_LEFT_1_SEQUENCE=AACTCCTGGCCACATGAAAC
PRIMER_RIGHT_1_SEQUENCE=TCCCCTGAAGTTCAATGTGC
PRIMER_INTERNAL_1_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
PRIMER_LEFT_1=123,20
PRIMER_RIGHT_1=216,20
PRIMER_INTERNAL_1=166,26
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=61.046
PRIMER_INTERNAL_1_TM=57.766
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=34.615
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=1.125695
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=1.014618
PRIMER_INTERNAL_2_PENALTY=8.234100
PRIMER_LEFT_2_SEQUENCE=CTCCTGGCCACATGAAACTT
PRIMER_RIGHT_2_SEQUENCE=CCCTGAAGTTCAATGTGCATT
PRIMER_INTERNAL_2_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
PRIMER_LEFT_2=125,20
PRIMER_RIGHT_2=214,21
PRIMER_INTERNAL_2=166,26
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=59.985
PRIMER_INTERNAL_2_TM=57.766
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=34.615
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=1.157044
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=1.045967
PRIMER_INTERNAL_3_PENALTY=8.234100
PRIMER_LEFT_3_SEQUENCE=CTCCTGGCCACATGAAACTT
PRIMER_RIGHT_3_SEQUENCE=TCCCCTGAAGTTCAATGTGC
PRIMER_INTERNAL_3_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT
PRIMER_LEFT_3=125,20
PRIMER_RIGHT_3=216,20
PRIMER_INTERNAL_3=166,26
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=61.046
PRIMER_INTERNAL_3_TM=57.766
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=34.615
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=7.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=1.353849
PRIMER_LEFT_4_PENALTY=0.339231
PRIMER_RIGHT_4_PENALTY=1.014618
PRIMER_INTERNAL_4_PENALTY=6.619157
PRIMER_LEFT_4_SEQUENCE=GATCTTGAACTCCTGGCCAC
PRIMER_RIGHT_4_SEQUENCE=CCCTGAAGTTCAATGTGCATT
PRIMER_INTERNAL_4_SEQUENCE=TGAAACTTATTAAAGGAAGTGACCA
PRIMER_LEFT_4=116,20
PRIMER_RIGHT_4=214,21
PRIMER_INTERNAL_4=137,25
PRIMER_LEFT_4_TM=59.661
PRIMER_RIGHT_4_TM=59.985
PRIMER_INTERNAL_4_TM=58.381
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH274
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAGTCACGTGTCACATGCAGTGTTTGCACTTGCCATTCATGGTTGTGAGGATTAAACAGACAAAAAGTNTACATAAAGAACCTAGCACGGCCACTGGCACGTAGTAGGAACACAAAAGAAAATGGTATTCCTTNTGATCTTTNTTATTTNATTTAGTCCTCATAATGCTTNTGTGNCAGCATTATCACCATTCCTGCACGAGGAAACAAGGCTTCAAAAGGATCAATAATTATGTAAACCCAAGGCTTGTGGGTGGCAGAGATCCGGCCGGAATTCA
SEQUENCE_INCLUDED_REGION=35,281
P3_COMMENT=CL is CACATGCAGTGTTTGCACTT
P3_COMMENT=CR is TTGATCCTTTTGAAGCCTTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.831026
PRIMER_LEFT_0_PENALTY=0.068789
PRIMER_RIGHT_0_PENALTY=0.762236
PRIMER_INTERNAL_0_PENALTY=0.119180
PRIMER_LEFT_0_SEQUENCE=CAGTCACGTGTCACATGCAG
PRIMER_RIGHT_0_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
PRIMER_INTERNAL_0_SEQUENCE=GTTTGCACTTGCCATTCATG
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=131,20
PRIMER_INTERNAL_0=60,20
PRIMER_LEFT_0_TM=59.931
PRIMER_RIGHT_0_TM=60.762
PRIMER_INTERNAL_0_TM=60.119
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=93
PRIMER_PAIR_1_PENALTY=0.831026
PRIMER_LEFT_1_PENALTY=0.068789
PRIMER_RIGHT_1_PENALTY=0.762236
PRIMER_INTERNAL_1_PENALTY=0.119180
PRIMER_LEFT_1_SEQUENCE=CTGCAGTCACGTGTCACATG
PRIMER_RIGHT_1_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
PRIMER_INTERNAL_1_SEQUENCE=GTTTGCACTTGCCATTCATG
PRIMER_LEFT_1=36,20
PRIMER_RIGHT_1=131,20
PRIMER_INTERNAL_1=60,20
PRIMER_LEFT_1_TM=59.931
PRIMER_RIGHT_1_TM=60.762
PRIMER_INTERNAL_1_TM=60.119
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=0.984730
PRIMER_LEFT_2_PENALTY=0.222493
PRIMER_RIGHT_2_PENALTY=0.762236
PRIMER_INTERNAL_2_PENALTY=0.516718
PRIMER_LEFT_2_SEQUENCE=CGTGTCACATGCAGTGTTTG
PRIMER_RIGHT_2_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
PRIMER_INTERNAL_2_SEQUENCE=TTGCCATTCATGGTTGTGAG
PRIMER_LEFT_2=45,20
PRIMER_RIGHT_2=131,20
PRIMER_INTERNAL_2=68,20
PRIMER_LEFT_2_TM=59.778
PRIMER_RIGHT_2_TM=60.762
PRIMER_INTERNAL_2_TM=60.517
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=0.990657
PRIMER_LEFT_3_PENALTY=0.228420
PRIMER_RIGHT_3_PENALTY=0.762236
PRIMER_INTERNAL_3_PENALTY=0.516718
PRIMER_LEFT_3_SEQUENCE=TCACGTGTCACATGCAGTGT
PRIMER_RIGHT_3_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
PRIMER_INTERNAL_3_SEQUENCE=TTGCCATTCATGGTTGTGAG
PRIMER_LEFT_3=42,20
PRIMER_RIGHT_3=131,20
PRIMER_INTERNAL_3=68,20
PRIMER_LEFT_3_TM=59.772
PRIMER_RIGHT_3_TM=60.762
PRIMER_INTERNAL_3_TM=60.517
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=0.990657
PRIMER_LEFT_4_PENALTY=0.228420
PRIMER_RIGHT_4_PENALTY=0.762236
PRIMER_INTERNAL_4_PENALTY=0.516718
PRIMER_LEFT_4_SEQUENCE=GTCACGTGTCACATGCAGTG
PRIMER_RIGHT_4_SEQUENCE=TGGCCGTGCTAGGTTCTTTA
PRIMER_INTERNAL_4_SEQUENCE=TTGCCATTCATGGTTGTGAG
PRIMER_LEFT_4=41,20
PRIMER_RIGHT_4=131,20
PRIMER_INTERNAL_4=68,20
PRIMER_LEFT_4_TM=59.772
PRIMER_RIGHT_4_TM=60.762
PRIMER_INTERNAL_4_TM=60.517
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH286
SEQUENCE_TEMPLATE=CTTCTTTTAAAAATCCTGTCAGCTGCAAAGGAAAGGAAGGACTAATATTTTCCACGAGATCAGTAACATCTTTATGAAGATGCTCCTTTTGAATGTGGTACAGAAAGTTGGAAAACACTTAGGCATGGTATCTGGCATAGTGCCCCTTAGGGAGCACTTCACATGCAGACGGTCTGGGGCTTAGGATGGTTTGACTTAGAATTTTTTGACTTTATGATAGTTGCAAAGGCGATGCACATTCAGTAGAAAGTGAACTTTGAGTCCCCATAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGT
SEQUENCE_INCLUDED_REGION=0,271
P3_COMMENT=CL is AAATCCTGTCAGCTGCAAAG
P3_COMMENT=CR is TTCTACTGAATGTGCATCGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.413785
PRIMER_LEFT_0_PENALTY=0.246438
PRIMER_RIGHT_0_PENALTY=0.167348
PRIMER_INTERNAL_0_PENALTY=0.327533
PRIMER_LEFT_0_SEQUENCE=CCCTTAGGGAGCACTTCACA
PRIMER_RIGHT_0_SEQUENCE=CATCGCCTTTGCAACTATCA
PRIMER_INTERNAL_0_SEQUENCE=GTCTGGGGCTTAGGATGGTT
PRIMER_LEFT_0=143,20
PRIMER_RIGHT_0=233,20
PRIMER_INTERNAL_0=171,20
PRIMER_LEFT_0_TM=60.246
PRIMER_RIGHT_0_TM=59.833
PRIMER_INTERNAL_0_TM=60.328
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=5.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=0.485929
PRIMER_LEFT_1_PENALTY=0.318581
PRIMER_RIGHT_1_PENALTY=0.167348
PRIMER_INTERNAL_1_PENALTY=0.692698
PRIMER_LEFT_1_SEQUENCE=CACTTCACATGCAGACGGTC
PRIMER_RIGHT_1_SEQUENCE=CATCGCCTTTGCAACTATCA
PRIMER_INTERNAL_1_SEQUENCE=GGGGCTTAGGATGGTTTGAC
PRIMER_LEFT_1=154,20
PRIMER_RIGHT_1=233,20
PRIMER_INTERNAL_1=175,20
PRIMER_LEFT_1_TM=60.319
PRIMER_RIGHT_1_TM=59.833
PRIMER_INTERNAL_1_TM=60.693
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=0.488912
PRIMER_LEFT_2_PENALTY=0.318581
PRIMER_RIGHT_2_PENALTY=0.170331
PRIMER_INTERNAL_2_PENALTY=0.692698
PRIMER_LEFT_2_SEQUENCE=CACTTCACATGCAGACGGTC
PRIMER_RIGHT_2_SEQUENCE=TCTACTGAATGTGCATCGCC
PRIMER_INTERNAL_2_SEQUENCE=GGGGCTTAGGATGGTTTGAC
PRIMER_LEFT_2=154,20
PRIMER_RIGHT_2=246,20
PRIMER_INTERNAL_2=175,20
PRIMER_LEFT_2_TM=60.319
PRIMER_RIGHT_2_TM=59.830
PRIMER_INTERNAL_2_TM=60.693
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=93
PRIMER_PAIR_3_PENALTY=0.497864
PRIMER_LEFT_3_PENALTY=0.327533
PRIMER_RIGHT_3_PENALTY=0.170331
PRIMER_INTERNAL_3_PENALTY=7.511946
PRIMER_LEFT_3_SEQUENCE=GTCTGGGGCTTAGGATGGTT
PRIMER_RIGHT_3_SEQUENCE=TCTACTGAATGTGCATCGCC
PRIMER_INTERNAL_3_SEQUENCE=TTTTTTGACTTTATGATAGTTGCAAA
PRIMER_LEFT_3=171,20
PRIMER_RIGHT_3=246,20
PRIMER_INTERNAL_3=201,26
PRIMER_LEFT_3_TM=60.328
PRIMER_RIGHT_3_TM=59.830
PRIMER_INTERNAL_3_TM=58.488
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=23.077
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=0.580576
PRIMER_LEFT_4_PENALTY=0.413229
PRIMER_RIGHT_4_PENALTY=0.167348
PRIMER_INTERNAL_4_PENALTY=0.327533
PRIMER_LEFT_4_SEQUENCE=TAGGGAGCACTTCACATGCA
PRIMER_RIGHT_4_SEQUENCE=CATCGCCTTTGCAACTATCA
PRIMER_INTERNAL_4_SEQUENCE=GTCTGGGGCTTAGGATGGTT
PRIMER_LEFT_4=147,20
PRIMER_RIGHT_4=233,20
PRIMER_INTERNAL_4=171,20
PRIMER_LEFT_4_TM=60.413
PRIMER_RIGHT_4_TM=59.833
PRIMER_INTERNAL_4_TM=60.328
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH288
SEQUENCE_TEMPLATE=CTCCACTTCCCGGGTTCNTGCCATTGTCCTGCCTCAGCGTCCTGAATAGGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
SEQUENCE_INCLUDED_REGION=0,49
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH293
SEQUENCE_TEMPLATE=CTACTGGAGAGAGAACTGGGATTCAAATCCAGGAGCCCGCTCTTCACTCCAACACTATATTGCTTACTTAATGGTTACAGATTAAGCCCAGAAAGGAAGCCTGTCTCAATACACTAGATATAGTTACTGTGATTATATATTTTAATAAATGGTCCTTTTATTAAAAAAAAAAAAGNTATCTAAAGAGAAAACCATAATAATCTCTCAGGTAATTATGGCCACAGCCAAAACCAGTCTTTCTAAACCTAAAGACTCTCATAAAGGCCCTATCACATAACTTCTCCACTTCCCAGTAAAGCATTGAGG
SEQUENCE_INCLUDED_REGION=0,306
P3_COMMENT=CL is CTCTTCACTCCAACACTATATTGC
P3_COMMENT=CR is TGCTTTACTGGGAAGTGGAG
P3_COMMENT=CM is TACTTAATGGTTACAGATTAAGCCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.853930
PRIMER_LEFT_0_PENALTY=0.521793
PRIMER_RIGHT_0_PENALTY=0.332137
PRIMER_INTERNAL_0_PENALTY=0.444121
PRIMER_LEFT_0_SEQUENCE=CTGGGATTCAAATCCAGGAG
PRIMER_RIGHT_0_SEQUENCE=AGGCTTCCTTTCTGGGCTTA
PRIMER_INTERNAL_0_SEQUENCE=CCGCTCTTCACTCCAACACT
PRIMER_LEFT_0=15,20
PRIMER_RIGHT_0=101,20
PRIMER_INTERNAL_0=36,20
PRIMER_LEFT_0_TM=59.478
PRIMER_RIGHT_0_TM=60.332
PRIMER_INTERNAL_0_TM=60.444
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=0.995685
PRIMER_LEFT_1_PENALTY=0.663548
PRIMER_RIGHT_1_PENALTY=0.332137
PRIMER_INTERNAL_1_PENALTY=0.444121
PRIMER_LEFT_1_SEQUENCE=ACTGGGATTCAAATCCAGGA
PRIMER_RIGHT_1_SEQUENCE=AGGCTTCCTTTCTGGGCTTA
PRIMER_INTERNAL_1_SEQUENCE=CCGCTCTTCACTCCAACACT
PRIMER_LEFT_1=14,20
PRIMER_RIGHT_1=101,20
PRIMER_INTERNAL_1=36,20
PRIMER_LEFT_1_TM=59.336
PRIMER_RIGHT_1_TM=60.332
PRIMER_INTERNAL_1_TM=60.444
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=1.204747
PRIMER_LEFT_2_PENALTY=0.521793
PRIMER_RIGHT_2_PENALTY=0.682954
PRIMER_INTERNAL_2_PENALTY=0.444121
PRIMER_LEFT_2_SEQUENCE=CTGGGATTCAAATCCAGGAG
PRIMER_RIGHT_2_SEQUENCE=GGCTTCCTTTCTGGGCTTAA
PRIMER_INTERNAL_2_SEQUENCE=CCGCTCTTCACTCCAACACT
PRIMER_LEFT_2=15,20
PRIMER_RIGHT_2=100,20
PRIMER_INTERNAL_2=36,20
PRIMER_LEFT_2_TM=59.478
PRIMER_RIGHT_2_TM=60.683
PRIMER_INTERNAL_2_TM=60.444
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=1.277882
PRIMER_LEFT_3_PENALTY=0.521793
PRIMER_RIGHT_3_PENALTY=0.756089
PRIMER_INTERNAL_3_PENALTY=0.444121
PRIMER_LEFT_3_SEQUENCE=CTGGGATTCAAATCCAGGAG
PRIMER_RIGHT_3_SEQUENCE=AGACAGGCTTCCTTTCTGGG
PRIMER_INTERNAL_3_SEQUENCE=CCGCTCTTCACTCCAACACT
PRIMER_LEFT_3=15,20
PRIMER_RIGHT_3=105,20
PRIMER_INTERNAL_3=36,20
PRIMER_LEFT_3_TM=59.478
PRIMER_RIGHT_3_TM=60.756
PRIMER_INTERNAL_3_TM=60.444
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=1.287701
PRIMER_LEFT_4_PENALTY=0.148509
PRIMER_RIGHT_4_PENALTY=1.139192
PRIMER_INTERNAL_4_PENALTY=3.342564
PRIMER_LEFT_4_SEQUENCE=CCACAGCCAAAACCAGTCTT
PRIMER_RIGHT_4_SEQUENCE=TGCTTTACTGGGAAGTGGAGA
PRIMER_INTERNAL_4_SEQUENCE=CCTAAAGACTCTCATAAAGGCCC
PRIMER_LEFT_4=218,20
PRIMER_RIGHT_4=299,21
PRIMER_INTERNAL_4=244,23
PRIMER_LEFT_4_TM=60.149
PRIMER_RIGHT_4_TM=59.861
PRIMER_INTERNAL_4_TM=59.657
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH295
SEQUENCE_TEMPLATE=CTGTGACCAATTGTTATTTTAGAGGGTTTAACAATGGCCTGACTATCACCTGATGGTCGCCAGAATTTCCTGGGGGAGGGCCTCCCCTGCCCTGATCATGTCTACCTAACTGCCTACTCTAACAATACTACTCCTGTGGTATGGGGATCCTAAGCCAAAAAGCTGAAATGAACATGTTCTAGCACTACAGAAATCCATACTGCCCCTCAGTAAAGGCAAATTTTAAATCTCTTTGGATAACCCAGGGCACATGTGAACAACCCCTTCTGAAGAAGCACTTATTCTTCTTCACACTCACCATGAAAA
SEQUENCE_INCLUDED_REGION=0,306
P3_COMMENT=CL is TGTGACCAATTGTTATTTTAGAGG
P3_COMMENT=CR is ATGGTGAGTGTGAAGAAGAATAA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.472761
PRIMER_LEFT_0_PENALTY=0.106310
PRIMER_RIGHT_0_PENALTY=0.366450
PRIMER_INTERNAL_0_PENALTY=0.816516
PRIMER_LEFT_0_SEQUENCE=GAAATCCATACTGCCCCTCA
PRIMER_RIGHT_0_SEQUENCE=TGCTTCTTCAGAAGGGGTTG
PRIMER_INTERNAL_0_SEQUENCE=CTCTTTGGATAACCCAGGGC
PRIMER_LEFT_0=189,20
PRIMER_RIGHT_0=276,20
PRIMER_INTERNAL_0=228,20
PRIMER_LEFT_0_TM=59.894
PRIMER_RIGHT_0_TM=60.366
PRIMER_INTERNAL_0_TM=60.817
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.483020
PRIMER_LEFT_1_PENALTY=0.376709
PRIMER_RIGHT_1_PENALTY=0.106310
PRIMER_INTERNAL_1_PENALTY=1.236972
PRIMER_LEFT_1_SEQUENCE=CCTGTGGTATGGGGATCCTA
PRIMER_RIGHT_1_SEQUENCE=TGAGGGGCAGTATGGATTTC
PRIMER_INTERNAL_1_SEQUENCE=GCCAAAAAGCTGAAATGAACA
PRIMER_LEFT_1=132,20
PRIMER_RIGHT_1=208,20
PRIMER_INTERNAL_1=153,21
PRIMER_LEFT_1_TM=59.623
PRIMER_RIGHT_1_TM=59.894
PRIMER_INTERNAL_1_TM=60.237
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=0.491775
PRIMER_LEFT_2_PENALTY=0.040562
PRIMER_RIGHT_2_PENALTY=0.451213
PRIMER_INTERNAL_2_PENALTY=5.263882
PRIMER_LEFT_2_SEQUENCE=GGGATCCTAAGCCAAAAAGC
PRIMER_RIGHT_2_SEQUENCE=ATTTGCCTTTACTGAGGGGC
PRIMER_INTERNAL_2_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA
PRIMER_LEFT_2=143,20
PRIMER_RIGHT_2=220,20
PRIMER_INTERNAL_2=172,25
PRIMER_LEFT_2_TM=60.041
PRIMER_RIGHT_2_TM=60.451
PRIMER_INTERNAL_2_TM=59.736
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=12.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=0.548000
PRIMER_LEFT_3_PENALTY=0.096788
PRIMER_RIGHT_3_PENALTY=0.451213
PRIMER_INTERNAL_3_PENALTY=5.263882
PRIMER_LEFT_3_SEQUENCE=GGGGATCCTAAGCCAAAAAG
PRIMER_RIGHT_3_SEQUENCE=ATTTGCCTTTACTGAGGGGC
PRIMER_INTERNAL_3_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA
PRIMER_LEFT_3=142,20
PRIMER_RIGHT_3=220,20
PRIMER_INTERNAL_3=172,25
PRIMER_LEFT_3_TM=59.903
PRIMER_RIGHT_3_TM=60.451
PRIMER_INTERNAL_3_TM=59.736
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=12.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=0.761112
PRIMER_LEFT_4_PENALTY=0.096788
PRIMER_RIGHT_4_PENALTY=0.664324
PRIMER_INTERNAL_4_PENALTY=5.263882
PRIMER_LEFT_4_SEQUENCE=GGGGATCCTAAGCCAAAAAG
PRIMER_RIGHT_4_SEQUENCE=GCCTTTACTGAGGGGCAGTA
PRIMER_INTERNAL_4_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA
PRIMER_LEFT_4=142,20
PRIMER_RIGHT_4=216,20
PRIMER_INTERNAL_4=172,25
PRIMER_LEFT_4_TM=59.903
PRIMER_RIGHT_4_TM=59.336
PRIMER_INTERNAL_4_TM=59.736
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH303
SEQUENCE_TEMPLATE=CTCACCATTACTTGCTCCACTGGGNCCCTGCAGGNATGCTGTGGTCCTCCCACTGGCAGGCTTCAGGGTACNGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCA
SEQUENCE_INCLUDED_REGION=0,65
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH315
SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTAGCCATTAGGACTTCCTGAAGACAAGGCAGGCAGAACAAAAGAGAAAGAGCAAAGGTTCACAGGCAAGAAAGGACCTAGCAGCATGTCCAAGAAACAGNAAAAGGCTTGTGTGACTCAAGTGGGAAATCATGGGAAACAGGTCTGATATGAGGCTAAGGATCCAGGCAAAAGCCAGACTGTAAAGGGCAGT
SEQUENCE_INCLUDED_REGION=31,193
P3_COMMENT=CL is GACTTCCTGAAGACAAGGCA
P3_COMMENT=CR is TTTACAGTCTGGCTTTTGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.433504
PRIMER_LEFT_0_PENALTY=0.308849
PRIMER_RIGHT_0_PENALTY=0.124655
PRIMER_INTERNAL_0_PENALTY=0.566932
PRIMER_LEFT_0_SEQUENCE=CTCAAGTGGGAAATCATGGG
PRIMER_RIGHT_0_SEQUENCE=TGCCCTTTACAGTCTGGCTT
PRIMER_INTERNAL_0_SEQUENCE=ATGAGGCTAAGGATCCAGGC
PRIMER_LEFT_0=147,20
PRIMER_RIGHT_0=221,20
PRIMER_INTERNAL_0=180,20
PRIMER_LEFT_0_TM=60.309
PRIMER_RIGHT_0_TM=59.875
PRIMER_INTERNAL_0_TM=60.567
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.464909
PRIMER_LEFT_1_PENALTY=0.156061
PRIMER_RIGHT_1_PENALTY=0.308849
PRIMER_INTERNAL_1_PENALTY=0.554836
PRIMER_LEFT_1_SEQUENCE=TCACAGGCAAGAAAGGACCT
PRIMER_RIGHT_1_SEQUENCE=CCCATGATTTCCCACTTGAG
PRIMER_INTERNAL_1_SEQUENCE=GCAGCATGTCCAAGAAACAG
PRIMER_LEFT_1=90,20
PRIMER_RIGHT_1=166,20
PRIMER_INTERNAL_1=111,20
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=60.309
PRIMER_INTERNAL_1_TM=59.445
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=0.541148
PRIMER_LEFT_2_PENALTY=0.232299
PRIMER_RIGHT_2_PENALTY=0.308849
PRIMER_INTERNAL_2_PENALTY=0.554836
PRIMER_LEFT_2_SEQUENCE=TTCACAGGCAAGAAAGGACC
PRIMER_RIGHT_2_SEQUENCE=CCCATGATTTCCCACTTGAG
PRIMER_INTERNAL_2_SEQUENCE=GCAGCATGTCCAAGAAACAG
PRIMER_LEFT_2=89,20
PRIMER_RIGHT_2=166,20
PRIMER_INTERNAL_2=111,20
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=60.309
PRIMER_INTERNAL_2_TM=59.445
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=0.541148
PRIMER_LEFT_3_PENALTY=0.232299
PRIMER_RIGHT_3_PENALTY=0.308849
PRIMER_INTERNAL_3_PENALTY=0.554836
PRIMER_LEFT_3_SEQUENCE=GGTTCACAGGCAAGAAAGGA
PRIMER_RIGHT_3_SEQUENCE=CCCATGATTTCCCACTTGAG
PRIMER_INTERNAL_3_SEQUENCE=GCAGCATGTCCAAGAAACAG
PRIMER_LEFT_3=87,20
PRIMER_RIGHT_3=166,20
PRIMER_INTERNAL_3=111,20
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=60.309
PRIMER_INTERNAL_3_TM=59.445
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=0.558158
PRIMER_LEFT_4_PENALTY=0.003322
PRIMER_RIGHT_4_PENALTY=0.554836
PRIMER_INTERNAL_4_PENALTY=0.156061
PRIMER_LEFT_4_SEQUENCE=GAAGACAAGGCAGGCAGAAC
PRIMER_RIGHT_4_SEQUENCE=TGTTTCTTGGACATGCTGCT
PRIMER_INTERNAL_4_SEQUENCE=TCACAGGCAAGAAAGGACCT
PRIMER_LEFT_4=50,20
PRIMER_RIGHT_4=129,20
PRIMER_INTERNAL_4=90,20
PRIMER_LEFT_4_TM=59.997
PRIMER_RIGHT_4_TM=59.445
PRIMER_INTERNAL_4_TM=59.844
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH318
SEQUENCE_TEMPLATE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGTTATTTTTCAGATTTGTTCATGTAGTTGCTTCGTAATGTCATTGGTATTTGCACTTAAGCGGAGGTAGTGGTTTTTCCTTTCCATATTTATTCATATTTATTATTTTCCTTTTTTTTTAAGTCACACATGAGTTTTTATTAAAGNCATATACTTACATTTTNAAAGCAAATATATTGACTAGTTCTTCTGAAAAATTGCTTGAAAAATGTAATTTTTTTT
SEQUENCE_INCLUDED_REGION=32,224
P3_COMMENT=CL is TATTTGCACTTAAGCGGAGG
P3_COMMENT=CR is ACATTTTTCAAGCAATTTTTCA
P3_COMMENT=CM is AGTGGTTTTTCCTTTCCATATTT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.512761
PRIMER_LEFT_0_PENALTY=5.657140
PRIMER_RIGHT_0_PENALTY=0.855622
PRIMER_INTERNAL_0_PENALTY=2.773104
PRIMER_LEFT_0_SEQUENCE=TGGTTATTTTTCAGATTTGTTCATG
PRIMER_RIGHT_0_SEQUENCE=GGAAAAACCACTACCTCCGC
PRIMER_INTERNAL_0_SEQUENCE=TTGCTTCGTAATGTCATTGG
PRIMER_LEFT_0=33,25
PRIMER_RIGHT_0=113,20
PRIMER_INTERNAL_0=61,20
PRIMER_LEFT_0_TM=59.343
PRIMER_RIGHT_0_TM=60.856
PRIMER_INTERNAL_0_TM=57.227
PRIMER_LEFT_0_GC_PERCENT=28.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=11.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=6.882352
PRIMER_LEFT_1_PENALTY=5.390387
PRIMER_RIGHT_1_PENALTY=1.491965
PRIMER_INTERNAL_1_PENALTY=3.252332
PRIMER_LEFT_1_SEQUENCE=TTTTCAGATTTGTTCATGTAGTTGC
PRIMER_RIGHT_1_SEQUENCE=AAGGAAAAACCACTACCTCCG
PRIMER_INTERNAL_1_SEQUENCE=TCGTAATGTCATTGGTATTTGCA
PRIMER_LEFT_1=40,25
PRIMER_RIGHT_1=115,21
PRIMER_INTERNAL_1=66,23
PRIMER_LEFT_1_TM=59.610
PRIMER_RIGHT_1_TM=59.508
PRIMER_INTERNAL_1_TM=60.252
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=6.978959
PRIMER_LEFT_2_PENALTY=1.136289
PRIMER_RIGHT_2_PENALTY=5.842670
PRIMER_INTERNAL_2_PENALTY=2.914618
PRIMER_LEFT_2_SEQUENCE=TTGGTATTTGCACTTAAGCGG
PRIMER_RIGHT_2_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA
PRIMER_INTERNAL_2_SEQUENCE=GGTAGTGGTTTTTCCTTTCCA
PRIMER_LEFT_2=77,21
PRIMER_RIGHT_2=166,25
PRIMER_INTERNAL_2=99,21
PRIMER_LEFT_2_TM=60.136
PRIMER_RIGHT_2_TM=59.157
PRIMER_INTERNAL_2_TM=58.085
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=28.000
PRIMER_INTERNAL_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=6.982128
PRIMER_LEFT_3_PENALTY=1.139458
PRIMER_RIGHT_3_PENALTY=5.842670
PRIMER_INTERNAL_3_PENALTY=5.718597
PRIMER_LEFT_3_SEQUENCE=ATTTGCACTTAAGCGGAGGT
PRIMER_RIGHT_3_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA
PRIMER_INTERNAL_3_SEQUENCE=AGTGGTTTTTCCTTTCCATATTT
PRIMER_LEFT_3=82,20
PRIMER_RIGHT_3=166,25
PRIMER_INTERNAL_3=102,23
PRIMER_LEFT_3_TM=58.861
PRIMER_RIGHT_3_TM=59.157
PRIMER_INTERNAL_3_TM=57.281
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=28.000
PRIMER_INTERNAL_3_GC_PERCENT=30.435
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=7.001609
PRIMER_LEFT_4_PENALTY=1.158939
PRIMER_RIGHT_4_PENALTY=5.842670
PRIMER_INTERNAL_4_PENALTY=2.914618
PRIMER_LEFT_4_SEQUENCE=TGGTATTTGCACTTAAGCGG
PRIMER_RIGHT_4_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA
PRIMER_INTERNAL_4_SEQUENCE=GGTAGTGGTTTTTCCTTTCCA
PRIMER_LEFT_4=78,20
PRIMER_RIGHT_4=166,25
PRIMER_INTERNAL_4=99,21
PRIMER_LEFT_4_TM=58.841
PRIMER_RIGHT_4_TM=59.157
PRIMER_INTERNAL_4_TM=58.085
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=28.000
PRIMER_INTERNAL_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH319
SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCTGAGAATCAAATCAAGAACTCAACCCCTTTCACAATAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTT
SEQUENCE_INCLUDED_REGION=31,37
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH323
SEQUENCE_TEMPLATE=TCATGCCTGAGGTCGACTCTAGAGGATCCCCCTTATTTTGAAAAATAGTTCATATATTTATAAACATCTTTTAATTCCATTTTTCCATGAACTTTTTGAACTCCTCATATTCTCTATATCTNTNTTTAAAAATTTACAAAAATAAAAACTTTTAAAAGTTATAAAAGAAATATTTTCATGTCAGATATAGGAAAAAATACAACANGGTCACCTAGAATTTTACCAGAACCACGTAGCAACCTCTAATCATTTTGTGTGCTTAATTTTAGTCATTTTTATTATTCTTAAGATGTTAAATAATTTAAA
SEQUENCE_INCLUDED_REGION=31,275
P3_COMMENT=CL is AACATCTTTTAATTCCATTTTTCC
P3_COMMENT=CR is CAAAATGATTAGAGGTTGCTACG
P3_COMMENT=CM is TGAACTTTTTGAACTCCTCATATTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.269945
PRIMER_LEFT_0_PENALTY=3.376837
PRIMER_RIGHT_0_PENALTY=3.893108
PRIMER_INTERNAL_0_PENALTY=9.512142
PRIMER_LEFT_0_SEQUENCE=TCCATGAACTTTTTGAACTCCTC
PRIMER_RIGHT_0_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGG
PRIMER_INTERNAL_0_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
PRIMER_LEFT_0=83,23
PRIMER_RIGHT_0=232,23
PRIMER_INTERNAL_0=175,27
PRIMER_LEFT_0_TM=59.623
PRIMER_RIGHT_0_TM=59.107
PRIMER_INTERNAL_0_TM=57.488
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=43.478
PRIMER_INTERNAL_0_GC_PERCENT=25.926
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=150
PRIMER_PAIR_1_PENALTY=7.392962
PRIMER_LEFT_1_PENALTY=3.499854
PRIMER_RIGHT_1_PENALTY=3.893108
PRIMER_INTERNAL_1_PENALTY=9.512142
PRIMER_LEFT_1_SEQUENCE=CCATGAACTTTTTGAACTCCTCA
PRIMER_RIGHT_1_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGG
PRIMER_INTERNAL_1_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
PRIMER_LEFT_1=84,23
PRIMER_RIGHT_1=232,23
PRIMER_INTERNAL_1=175,27
PRIMER_LEFT_1_TM=60.500
PRIMER_RIGHT_1_TM=59.107
PRIMER_INTERNAL_1_TM=57.488
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_INTERNAL_1_GC_PERCENT=25.926
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=149
PRIMER_PAIR_2_PENALTY=7.537689
PRIMER_LEFT_2_PENALTY=3.376837
PRIMER_RIGHT_2_PENALTY=4.160852
PRIMER_INTERNAL_2_PENALTY=9.512142
PRIMER_LEFT_2_SEQUENCE=TCCATGAACTTTTTGAACTCCTC
PRIMER_RIGHT_2_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGGT
PRIMER_INTERNAL_2_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
PRIMER_LEFT_2=83,23
PRIMER_RIGHT_2=232,24
PRIMER_INTERNAL_2=175,27
PRIMER_LEFT_2_TM=59.623
PRIMER_RIGHT_2_TM=59.839
PRIMER_INTERNAL_2_TM=57.488
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_INTERNAL_2_GC_PERCENT=25.926
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=150
PRIMER_PAIR_3_PENALTY=7.660707
PRIMER_LEFT_3_PENALTY=3.499854
PRIMER_RIGHT_3_PENALTY=4.160852
PRIMER_INTERNAL_3_PENALTY=9.512142
PRIMER_LEFT_3_SEQUENCE=CCATGAACTTTTTGAACTCCTCA
PRIMER_RIGHT_3_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGGT
PRIMER_INTERNAL_3_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
PRIMER_LEFT_3=84,23
PRIMER_RIGHT_3=232,24
PRIMER_INTERNAL_3=175,27
PRIMER_LEFT_3_TM=60.500
PRIMER_RIGHT_3_TM=59.839
PRIMER_INTERNAL_3_TM=57.488
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_INTERNAL_3_GC_PERCENT=25.926
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=149
PRIMER_PAIR_4_PENALTY=7.660707
PRIMER_LEFT_4_PENALTY=3.499854
PRIMER_RIGHT_4_PENALTY=4.160852
PRIMER_INTERNAL_4_PENALTY=9.512142
PRIMER_LEFT_4_SEQUENCE=CCATGAACTTTTTGAACTCCTCA
PRIMER_RIGHT_4_SEQUENCE=ACGTGGTTCTGGTAAAATTCTAGG
PRIMER_INTERNAL_4_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA
PRIMER_LEFT_4=84,23
PRIMER_RIGHT_4=233,24
PRIMER_INTERNAL_4=175,27
PRIMER_LEFT_4_TM=60.500
PRIMER_RIGHT_4_TM=59.839
PRIMER_INTERNAL_4_TM=57.488
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_INTERNAL_4_GC_PERCENT=25.926
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=150
=
SEQUENCE_ID=MH328
SEQUENCE_TEMPLATE=NCTTCATGCTNAGGTCGACTCTAGAGGATCCCCTAATTAACATATGCATTATCTCACTTATCTCTTTTTTGTGGTGAGAACACTTAAAATCTAAGAATGATCAATTTCAAATAAAGATGGTAGTGAGCGAACAGAAGAGGTTTCATTGACTCCTAAACTGAGTACTCAAAAACGAGCAGGTGCTCACAGTCAGGAAGCAGGTGCTGAGTACAGGATGGGAAAAAGCATGGGGGTGTGCACTTGTCAAGAAAATGACAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG
SEQUENCE_INCLUDED_REGION=33,190
P3_COMMENT=CL is TCACTTATCTCTTTTTTGTGGTG
P3_COMMENT=CR is ATCCTGTACTCAGCACCTGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.415468
PRIMER_LEFT_0_PENALTY=1.393460
PRIMER_RIGHT_0_PENALTY=0.022009
PRIMER_INTERNAL_0_PENALTY=0.440316
PRIMER_LEFT_0_SEQUENCE=GAGCGAACAGAAGAGGTTTCA
PRIMER_RIGHT_0_SEQUENCE=CACCTGCTTCCTGACTGTGA
PRIMER_INTERNAL_0_SEQUENCE=ACTCAAAAACGAGCAGGTGC
PRIMER_LEFT_0=124,21
PRIMER_RIGHT_0=202,20
PRIMER_INTERNAL_0=163,20
PRIMER_LEFT_0_TM=59.607
PRIMER_RIGHT_0_TM=60.022
PRIMER_INTERNAL_0_TM=60.440
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=79
PRIMER_PAIR_1_PENALTY=1.415468
PRIMER_LEFT_1_PENALTY=1.393460
PRIMER_RIGHT_1_PENALTY=0.022009
PRIMER_INTERNAL_1_PENALTY=0.440316
PRIMER_LEFT_1_SEQUENCE=TGAGCGAACAGAAGAGGTTTC
PRIMER_RIGHT_1_SEQUENCE=CACCTGCTTCCTGACTGTGA
PRIMER_INTERNAL_1_SEQUENCE=ACTCAAAAACGAGCAGGTGC
PRIMER_LEFT_1=123,21
PRIMER_RIGHT_1=202,20
PRIMER_INTERNAL_1=163,20
PRIMER_LEFT_1_TM=59.607
PRIMER_RIGHT_1_TM=60.022
PRIMER_INTERNAL_1_TM=60.440
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=1.450346
PRIMER_LEFT_2_PENALTY=1.393460
PRIMER_RIGHT_2_PENALTY=0.056887
PRIMER_INTERNAL_2_PENALTY=0.440316
PRIMER_LEFT_2_SEQUENCE=GAGCGAACAGAAGAGGTTTCA
PRIMER_RIGHT_2_SEQUENCE=AGCACCTGCTTCCTGACTGT
PRIMER_INTERNAL_2_SEQUENCE=ACTCAAAAACGAGCAGGTGC
PRIMER_LEFT_2=124,21
PRIMER_RIGHT_2=204,20
PRIMER_INTERNAL_2=163,20
PRIMER_LEFT_2_TM=59.607
PRIMER_RIGHT_2_TM=60.057
PRIMER_INTERNAL_2_TM=60.440
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=1.450346
PRIMER_LEFT_3_PENALTY=1.393460
PRIMER_RIGHT_3_PENALTY=0.056887
PRIMER_INTERNAL_3_PENALTY=0.440316
PRIMER_LEFT_3_SEQUENCE=TGAGCGAACAGAAGAGGTTTC
PRIMER_RIGHT_3_SEQUENCE=AGCACCTGCTTCCTGACTGT
PRIMER_INTERNAL_3_SEQUENCE=ACTCAAAAACGAGCAGGTGC
PRIMER_LEFT_3=123,21
PRIMER_RIGHT_3=204,20
PRIMER_INTERNAL_3=163,20
PRIMER_LEFT_3_TM=59.607
PRIMER_RIGHT_3_TM=60.057
PRIMER_INTERNAL_3_TM=60.440
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=1.580464
PRIMER_LEFT_4_PENALTY=1.393460
PRIMER_RIGHT_4_PENALTY=0.187005
PRIMER_INTERNAL_4_PENALTY=4.466328
PRIMER_LEFT_4_SEQUENCE=GAGCGAACAGAAGAGGTTTCA
PRIMER_RIGHT_4_SEQUENCE=TGCTTCCTGACTGTGAGCAC
PRIMER_INTERNAL_4_SEQUENCE=AAACTGAGTACTCAAAAACGAGCA
PRIMER_LEFT_4=124,21
PRIMER_RIGHT_4=198,20
PRIMER_INTERNAL_4=154,24
PRIMER_LEFT_4_TM=59.607
PRIMER_RIGHT_4_TM=60.187
PRIMER_INTERNAL_4_TM=59.534
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH331
SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTATACATTTNAATATTGACTGCAATTGCACGTCCTATATCAATACTCACCTGTGTTTTTTAAACTGTGAACCAGTAAAGCATGATGATGATTGGCATCCCTCTGCAGTTAAAAAGAAGCCTAATAGTGTTAAAAATTCAATTCATCCAACACAAAGTTCTAAAATTTTNATGCCTTATAGAATTACTTGAGCACTCTCCAAAATCCCATATCCATGTCACAACCAATATTAAGTCCAAATATAACCAGTCCTTTTCTTTTTAATAGTCTTCATTTTTTTANGAGCAGTTTGAAATCCACAGCAAAGGGGTACCGA
SEQUENCE_INCLUDED_REGION=31,275
P3_COMMENT=CL is ACTGCAATTGCACGTCCTAT
P3_COMMENT=CR is AGACTATTAAAAAGAAAAGGACTGG
P3_COMMENT=CM is TGTTTTTTAAACTGTGAACCAGTAA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.169955
PRIMER_LEFT_0_PENALTY=0.129070
PRIMER_RIGHT_0_PENALTY=0.040885
PRIMER_INTERNAL_0_PENALTY=4.945226
PRIMER_LEFT_0_SEQUENCE=GACTGCAATTGCACGTCCTA
PRIMER_RIGHT_0_SEQUENCE=TGCCAATCATCATCATGCTT
PRIMER_INTERNAL_0_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
PRIMER_LEFT_0=48,20
PRIMER_RIGHT_0=127,20
PRIMER_INTERNAL_0=80,24
PRIMER_LEFT_0_TM=59.871
PRIMER_RIGHT_0_TM=60.041
PRIMER_INTERNAL_0_TM=59.055
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=8.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=0.273745
PRIMER_LEFT_1_PENALTY=0.129070
PRIMER_RIGHT_1_PENALTY=0.144675
PRIMER_INTERNAL_1_PENALTY=4.945226
PRIMER_LEFT_1_SEQUENCE=GACTGCAATTGCACGTCCTA
PRIMER_RIGHT_1_SEQUENCE=GATGCCAATCATCATCATGC
PRIMER_INTERNAL_1_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
PRIMER_LEFT_1=48,20
PRIMER_RIGHT_1=129,20
PRIMER_INTERNAL_1=80,24
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=59.855
PRIMER_INTERNAL_1_TM=59.055
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.342575
PRIMER_LEFT_2_PENALTY=0.129070
PRIMER_RIGHT_2_PENALTY=0.213506
PRIMER_INTERNAL_2_PENALTY=3.010529
PRIMER_LEFT_2_SEQUENCE=GACTGCAATTGCACGTCCTA
PRIMER_RIGHT_2_SEQUENCE=TTTAACTGCAGAGGGATGCC
PRIMER_INTERNAL_2_SEQUENCE=TGAACCAGTAAAGCATGATGATG
PRIMER_LEFT_2=48,20
PRIMER_RIGHT_2=143,20
PRIMER_INTERNAL_2=98,23
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.214
PRIMER_INTERNAL_2_TM=60.011
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.381318
PRIMER_LEFT_3_PENALTY=0.129070
PRIMER_RIGHT_3_PENALTY=0.252249
PRIMER_INTERNAL_3_PENALTY=4.945226
PRIMER_LEFT_3_SEQUENCE=GACTGCAATTGCACGTCCTA
PRIMER_RIGHT_3_SEQUENCE=GAGGGATGCCAATCATCATC
PRIMER_INTERNAL_3_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
PRIMER_LEFT_3=48,20
PRIMER_RIGHT_3=133,20
PRIMER_INTERNAL_3=80,24
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=60.252
PRIMER_INTERNAL_3_TM=59.055
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=0.434388
PRIMER_LEFT_4_PENALTY=0.129070
PRIMER_RIGHT_4_PENALTY=0.305318
PRIMER_INTERNAL_4_PENALTY=4.945226
PRIMER_LEFT_4_SEQUENCE=GACTGCAATTGCACGTCCTA
PRIMER_RIGHT_4_SEQUENCE=GGATGCCAATCATCATCATG
PRIMER_INTERNAL_4_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC
PRIMER_LEFT_4=48,20
PRIMER_RIGHT_4=130,20
PRIMER_INTERNAL_4=80,24
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=59.695
PRIMER_INTERNAL_4_TM=59.055
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=12.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=83
=
SEQUENCE_ID=MH333
SEQUENCE_TEMPLATE=NNCCANNNGGTCGACTCTAGAGGATCCCCCTAATCCTGATCTATTCAAATCTTAACAACTTAAGTTGATGGAGTNGACTTCTTCTAAATTAGTNATTCCCTAATTTGCCTGACTGTTGGAATTTCCAGGGCATGTTGAAAATACACATTATCCAGACTCTTACCTCTGCAGATCTATTTAGTGGTTCTAAAATGGCAGCCAGGAGTCTGGATTTTTCCCAGGGGTCCTGTGTAATTCACACTGATGAACAGGCAAGTTTGGGAAATAGTGCCTTAAGGAGATTTTTCATTAAGCAGTCTTCATTTGAAATGAGG
SEQUENCE_INCLUDED_REGION=29,285
P3_COMMENT=CL is ATCCTGATCTATTCAAATCTTAACA
P3_COMMENT=CR is AAGACTGCTTAATGAAAAATCTCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.301918
PRIMER_LEFT_0_PENALTY=0.213506
PRIMER_RIGHT_0_PENALTY=0.088413
PRIMER_INTERNAL_0_PENALTY=0.740815
PRIMER_LEFT_0_SEQUENCE=TAAAATGGCAGCCAGGAGTC
PRIMER_RIGHT_0_SEQUENCE=TTTCCCAAACTTGCCTGTTC
PRIMER_INTERNAL_0_SEQUENCE=CCAGGGGTCCTGTGTAATTC
PRIMER_LEFT_0=187,20
PRIMER_RIGHT_0=264,20
PRIMER_INTERNAL_0=217,20
PRIMER_LEFT_0_TM=60.214
PRIMER_RIGHT_0_TM=60.088
PRIMER_INTERNAL_0_TM=59.259
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=0.380827
PRIMER_LEFT_1_PENALTY=0.292414
PRIMER_RIGHT_1_PENALTY=0.088413
PRIMER_INTERNAL_1_PENALTY=0.298276
PRIMER_LEFT_1_SEQUENCE=GTTCTAAAATGGCAGCCAGG
PRIMER_RIGHT_1_SEQUENCE=TTTCCCAAACTTGCCTGTTC
PRIMER_INTERNAL_1_SEQUENCE=AGTCTGGATTTTTCCCAGGG
PRIMER_LEFT_1=183,20
PRIMER_RIGHT_1=264,20
PRIMER_INTERNAL_1=203,20
PRIMER_LEFT_1_TM=59.708
PRIMER_RIGHT_1_TM=60.088
PRIMER_INTERNAL_1_TM=60.298
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.380827
PRIMER_LEFT_2_PENALTY=0.292414
PRIMER_RIGHT_2_PENALTY=0.088413
PRIMER_INTERNAL_2_PENALTY=0.298276
PRIMER_LEFT_2_SEQUENCE=GGTTCTAAAATGGCAGCCAG
PRIMER_RIGHT_2_SEQUENCE=TTTCCCAAACTTGCCTGTTC
PRIMER_INTERNAL_2_SEQUENCE=AGTCTGGATTTTTCCCAGGG
PRIMER_LEFT_2=182,20
PRIMER_RIGHT_2=264,20
PRIMER_INTERNAL_2=203,20
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=60.088
PRIMER_INTERNAL_2_TM=60.298
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.432363
PRIMER_LEFT_3_PENALTY=0.343950
PRIMER_RIGHT_3_PENALTY=0.088413
PRIMER_INTERNAL_3_PENALTY=0.740815
PRIMER_LEFT_3_SEQUENCE=TCTAAAATGGCAGCCAGGAG
PRIMER_RIGHT_3_SEQUENCE=TTTCCCAAACTTGCCTGTTC
PRIMER_INTERNAL_3_SEQUENCE=CCAGGGGTCCTGTGTAATTC
PRIMER_LEFT_3=185,20
PRIMER_RIGHT_3=264,20
PRIMER_INTERNAL_3=217,20
PRIMER_LEFT_3_TM=60.344
PRIMER_RIGHT_3_TM=60.088
PRIMER_INTERNAL_3_TM=59.259
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=0.514222
PRIMER_LEFT_4_PENALTY=0.425809
PRIMER_RIGHT_4_PENALTY=0.088413
PRIMER_INTERNAL_4_PENALTY=0.298276
PRIMER_LEFT_4_SEQUENCE=GTGGTTCTAAAATGGCAGCC
PRIMER_RIGHT_4_SEQUENCE=TTTCCCAAACTTGCCTGTTC
PRIMER_INTERNAL_4_SEQUENCE=AGTCTGGATTTTTCCCAGGG
PRIMER_LEFT_4=180,20
PRIMER_RIGHT_4=264,20
PRIMER_INTERNAL_4=203,20
PRIMER_LEFT_4_TM=59.574
PRIMER_RIGHT_4_TM=60.088
PRIMER_INTERNAL_4_TM=60.298
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH335
SEQUENCE_TEMPLATE=NATGCTGAGGTCGACTCTAGAGGATCCCCTCAGATTTCTTCTCTACGTTGGTTAATGAGTNCCTTTTCAAACGGTTATATTTTGGGGTTGAGGTAAAGGTTTTTAGAGTNNTTCCAATCGTGTATGTAAGCAGTCAACTATTTNCCCTATGTGTAGGTCTGGCACCACAGACCTGTAGAAGTCTTCTCTTTAATTGTACCATTATTTTGAATGTAAAAAAAAANCAANTAAACATGTTGTTGTCATTGANCTGGCTGTAGATGCATACATAAG
SEQUENCE_INCLUDED_REGION=29,244
P3_COMMENT=CL is TCAGATTTCTTCTCTACGTTGG
P3_COMMENT=CR is TTCTACAGGTCTGTGGTGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.387185
PRIMER_LEFT_0_PENALTY=1.077784
PRIMER_RIGHT_0_PENALTY=0.309402
PRIMER_INTERNAL_0_PENALTY=3.067516
PRIMER_LEFT_0_SEQUENCE=TTTTGGGGTTGAGGTAAAGG
PRIMER_RIGHT_0_SEQUENCE=TCTGTGGTGCCAGACCTACA
PRIMER_INTERNAL_0_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
PRIMER_LEFT_0=79,20
PRIMER_RIGHT_0=170,20
PRIMER_INTERNAL_0=113,23
PRIMER_LEFT_0_TM=58.922
PRIMER_RIGHT_0_TM=60.309
PRIMER_INTERNAL_0_TM=60.068
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=1.387185
PRIMER_LEFT_1_PENALTY=1.077784
PRIMER_RIGHT_1_PENALTY=0.309402
PRIMER_INTERNAL_1_PENALTY=3.067516
PRIMER_LEFT_1_SEQUENCE=TTTTGGGGTTGAGGTAAAGG
PRIMER_RIGHT_1_SEQUENCE=TACAGGTCTGTGGTGCCAGA
PRIMER_INTERNAL_1_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
PRIMER_LEFT_1=79,20
PRIMER_RIGHT_1=176,20
PRIMER_INTERNAL_1=113,23
PRIMER_LEFT_1_TM=58.922
PRIMER_RIGHT_1_TM=60.309
PRIMER_INTERNAL_1_TM=60.068
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=98
PRIMER_PAIR_2_PENALTY=1.387185
PRIMER_LEFT_2_PENALTY=1.077784
PRIMER_RIGHT_2_PENALTY=0.309402
PRIMER_INTERNAL_2_PENALTY=3.067516
PRIMER_LEFT_2_SEQUENCE=TTTTGGGGTTGAGGTAAAGG
PRIMER_RIGHT_2_SEQUENCE=TCTACAGGTCTGTGGTGCCA
PRIMER_INTERNAL_2_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
PRIMER_LEFT_2=79,20
PRIMER_RIGHT_2=178,20
PRIMER_INTERNAL_2=113,23
PRIMER_LEFT_2_TM=58.922
PRIMER_RIGHT_2_TM=60.309
PRIMER_INTERNAL_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=1.581709
PRIMER_LEFT_3_PENALTY=1.272307
PRIMER_RIGHT_3_PENALTY=0.309402
PRIMER_INTERNAL_3_PENALTY=3.067516
PRIMER_LEFT_3_SEQUENCE=TGGGGTTGAGGTAAAGGTTTT
PRIMER_RIGHT_3_SEQUENCE=TCTGTGGTGCCAGACCTACA
PRIMER_INTERNAL_3_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
PRIMER_LEFT_3=82,21
PRIMER_RIGHT_3=170,20
PRIMER_INTERNAL_3=113,23
PRIMER_LEFT_3_TM=59.728
PRIMER_RIGHT_3_TM=60.309
PRIMER_INTERNAL_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=1.581709
PRIMER_LEFT_4_PENALTY=1.272307
PRIMER_RIGHT_4_PENALTY=0.309402
PRIMER_INTERNAL_4_PENALTY=3.067516
PRIMER_LEFT_4_SEQUENCE=TGGGGTTGAGGTAAAGGTTTT
PRIMER_RIGHT_4_SEQUENCE=TACAGGTCTGTGGTGCCAGA
PRIMER_INTERNAL_4_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA
PRIMER_LEFT_4=82,21
PRIMER_RIGHT_4=176,20
PRIMER_INTERNAL_4=113,23
PRIMER_LEFT_4_TM=59.728
PRIMER_RIGHT_4_TM=60.309
PRIMER_INTERNAL_4_TM=60.068
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH336
SEQUENCE_TEMPLATE=GCCTGCAGGTCGACTCTAGAGGATCCCACCTATCCCATTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
SEQUENCE_INCLUDED_REGION=27,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH341
SEQUENCE_TEMPLATE=CGTTCATGCTCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGGNCAAGTACAGCATGAAGGAATAATACTGTGGAAGTATAAACAGCAGGCTCCAGGCTCATCAGAAGAAAGCCTTACTGTGTACTTCTCTTTAAAAAAAAATATGTGAAATCAGATATTTATAGGAATTAGATATGTATTTNCATATTGATACATTTTGTGTATTATATGGGATTCCTGATATATCTATGTAGTATGGTTTCTNATACATAANNTATCTGATTTCATATGT
SEQUENCE_INCLUDED_REGION=33,239
P3_COMMENT=CL is ACTGTGGAAGTATAAACAGCAGG
P3_COMMENT=CR is ATCAGGAATCCCATATAATACACA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=11.127070
PRIMER_LEFT_0_PENALTY=4.081412
PRIMER_RIGHT_0_PENALTY=7.045658
PRIMER_INTERNAL_0_PENALTY=1.664117
PRIMER_LEFT_0_SEQUENCE=CAGCATGAAGGAATAATACTGTGG
PRIMER_RIGHT_0_SEQUENCE=AGTACACAGTAAGGCTTTCTTCTGA
PRIMER_INTERNAL_0_SEQUENCE=ATAAACAGCAGGCTCCAGGC
PRIMER_LEFT_0=50,24
PRIMER_RIGHT_0=125,25
PRIMER_INTERNAL_0=78,20
PRIMER_LEFT_0_TM=59.919
PRIMER_RIGHT_0_TM=57.954
PRIMER_INTERNAL_0_TM=61.664
PRIMER_LEFT_0_GC_PERCENT=41.667
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=11.522908
PRIMER_LEFT_1_PENALTY=4.081412
PRIMER_RIGHT_1_PENALTY=7.441496
PRIMER_INTERNAL_1_PENALTY=1.664117
PRIMER_LEFT_1_SEQUENCE=CAGCATGAAGGAATAATACTGTGG
PRIMER_RIGHT_1_SEQUENCE=AAGTACACAGTAAGGCTTTCTTCTG
PRIMER_INTERNAL_1_SEQUENCE=ATAAACAGCAGGCTCCAGGC
PRIMER_LEFT_1=50,24
PRIMER_RIGHT_1=126,25
PRIMER_INTERNAL_1=78,20
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=57.559
PRIMER_INTERNAL_1_TM=61.664
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=6.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=11.565640
PRIMER_LEFT_2_PENALTY=5.632647
PRIMER_RIGHT_2_PENALTY=5.932992
PRIMER_INTERNAL_2_PENALTY=1.664117
PRIMER_LEFT_2_SEQUENCE=ACAGCATGAAGGAATAATACTGTGG
PRIMER_RIGHT_2_SEQUENCE=TACACAGTAAGGCTTTCTTCTGATG
PRIMER_INTERNAL_2_SEQUENCE=ATAAACAGCAGGCTCCAGGC
PRIMER_LEFT_2=49,25
PRIMER_RIGHT_2=123,25
PRIMER_INTERNAL_2=78,20
PRIMER_LEFT_2_TM=60.633
PRIMER_RIGHT_2_TM=59.067
PRIMER_INTERNAL_2_TM=61.664
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=7.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=11.706451
PRIMER_LEFT_3_PENALTY=4.081412
PRIMER_RIGHT_3_PENALTY=7.625039
PRIMER_INTERNAL_3_PENALTY=1.664117
PRIMER_LEFT_3_SEQUENCE=CAGCATGAAGGAATAATACTGTGG
PRIMER_RIGHT_3_SEQUENCE=GTACACAGTAAGGCTTTCTTCTGAT
PRIMER_INTERNAL_3_SEQUENCE=ATAAACAGCAGGCTCCAGGC
PRIMER_LEFT_3=50,24
PRIMER_RIGHT_3=124,25
PRIMER_INTERNAL_3=78,20
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=57.375
PRIMER_INTERNAL_3_TM=61.664
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=11.963614
PRIMER_LEFT_4_PENALTY=4.917956
PRIMER_RIGHT_4_PENALTY=7.045658
PRIMER_INTERNAL_4_PENALTY=1.664117
PRIMER_LEFT_4_SEQUENCE=AGCATGAAGGAATAATACTGTGGA
PRIMER_RIGHT_4_SEQUENCE=AGTACACAGTAAGGCTTTCTTCTGA
PRIMER_INTERNAL_4_SEQUENCE=ATAAACAGCAGGCTCCAGGC
PRIMER_LEFT_4=51,24
PRIMER_RIGHT_4=125,25
PRIMER_INTERNAL_4=78,20
PRIMER_LEFT_4_TM=59.082
PRIMER_RIGHT_4_TM=57.954
PRIMER_INTERNAL_4_TM=61.664
PRIMER_LEFT_4_GC_PERCENT=37.500
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH343
SEQUENCE_TEMPLATE=GTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATCTTGTNTGCTCCTTACTGTACAGGTGGCTGACAAAGAGAAGGGAAGATGGAGCCGCCATCTTGAACATGATTGACGCAGGGTNCCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC
SEQUENCE_INCLUDED_REGION=33,82
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH345
SEQUENCE_TEMPLATE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTTCATAACTAGTNAGGGCCTTCATTCTATCTGAATAGATTNTAGCAAACCATCTATTCCATGCAGATAAATTCAGTCAATTAGGGTCCGNGCTCGAATTCGTAATCATGGTCATAGCTNTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGCGCCGGAAGCATAANGTGTAAAGCCTGGGNTGCCTAATGAGNGTGCTAACTCACA
SEQUENCE_INCLUDED_REGION=32,83
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH346
SEQUENCE_TEMPLATE=GTTCATGCTCAGGTCGACTCTAGAGGATCCCCTCATTGTGTTATGGTCAAAAAAAAAGTTTTTCAGGGGCAAATATGTGCTTCATTTATGGTAAATTTGTGTTCTCCAACTTTATTTACTTAATGTATTTCTNGTTCTTCTGCATATACAATGNNNTATCATTTCAAGGCTGCTTTGTAGTGTAATCTCTATGCANACATGTTAAGTAAAGCANATTAAGATGCACCATGTATTGTGCAGTCAGTGCAAATAGCCTAGGTGACAATCTGNTGANTGGNTAGATGTCTTCCACCTTGGGCCAACTTCACATANTTGTAAGGGAAGACA
SEQUENCE_INCLUDED_REGION=32,295
P3_COMMENT=CL is AAAGTTTTTCAGGGGCAAAT
P3_COMMENT=CR is AGGCTATTTGCACTGACTGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.894088
PRIMER_LEFT_0_PENALTY=1.010701
PRIMER_RIGHT_0_PENALTY=0.883387
PRIMER_INTERNAL_0_PENALTY=2.095538
PRIMER_LEFT_0_SEQUENCE=GATGCACCATGTATTGTGCAG
PRIMER_RIGHT_0_SEQUENCE=TATGTGAAGTTGGCCCAAGG
PRIMER_INTERNAL_0_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA
PRIMER_LEFT_0=219,21
PRIMER_RIGHT_0=310,20
PRIMER_INTERNAL_0=240,22
PRIMER_LEFT_0_TM=60.011
PRIMER_RIGHT_0_TM=60.883
PRIMER_INTERNAL_0_TM=59.904
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=10.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=2.310635
PRIMER_LEFT_1_PENALTY=1.427248
PRIMER_RIGHT_1_PENALTY=0.883387
PRIMER_INTERNAL_1_PENALTY=2.548500
PRIMER_LEFT_1_SEQUENCE=TGCACCATGTATTGTGCAGT
PRIMER_RIGHT_1_SEQUENCE=TATGTGAAGTTGGCCCAAGG
PRIMER_INTERNAL_1_SEQUENCE=TGCAAATAGCCTAGGTGACAA
PRIMER_LEFT_1=221,20
PRIMER_RIGHT_1=310,20
PRIMER_INTERNAL_1=244,21
PRIMER_LEFT_1_TM=58.573
PRIMER_RIGHT_1_TM=60.883
PRIMER_INTERNAL_1_TM=58.451
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=2.440107
PRIMER_LEFT_2_PENALTY=1.010701
PRIMER_RIGHT_2_PENALTY=1.429406
PRIMER_INTERNAL_2_PENALTY=2.095538
PRIMER_LEFT_2_SEQUENCE=GATGCACCATGTATTGTGCAG
PRIMER_RIGHT_2_SEQUENCE=GCCCAAGGTGGAAGACATCT
PRIMER_INTERNAL_2_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA
PRIMER_LEFT_2=219,21
PRIMER_RIGHT_2=298,20
PRIMER_INTERNAL_2=240,22
PRIMER_LEFT_2_TM=60.011
PRIMER_RIGHT_2_TM=61.429
PRIMER_INTERNAL_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=8.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=2.478967
PRIMER_LEFT_3_PENALTY=1.010701
PRIMER_RIGHT_3_PENALTY=1.468266
PRIMER_INTERNAL_3_PENALTY=2.095538
PRIMER_LEFT_3_SEQUENCE=GATGCACCATGTATTGTGCAG
PRIMER_RIGHT_3_SEQUENCE=GCCCAAGGTGGAAGACATC
PRIMER_INTERNAL_3_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA
PRIMER_LEFT_3=219,21
PRIMER_RIGHT_3=298,19
PRIMER_INTERNAL_3=240,22
PRIMER_LEFT_3_TM=60.011
PRIMER_RIGHT_3_TM=60.468
PRIMER_INTERNAL_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=2.483650
PRIMER_LEFT_4_PENALTY=1.600264
PRIMER_RIGHT_4_PENALTY=0.883387
PRIMER_INTERNAL_4_PENALTY=2.548500
PRIMER_LEFT_4_SEQUENCE=GCACCATGTATTGTGCAGTCA
PRIMER_RIGHT_4_SEQUENCE=TATGTGAAGTTGGCCCAAGG
PRIMER_INTERNAL_4_SEQUENCE=TGCAAATAGCCTAGGTGACAA
PRIMER_LEFT_4=222,21
PRIMER_RIGHT_4=310,20
PRIMER_INTERNAL_4=244,21
PRIMER_LEFT_4_TM=60.600
PRIMER_RIGHT_4_TM=60.883
PRIMER_INTERNAL_4_TM=58.451
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH347
SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCTATCTGTNNATCTTGCCTCTGAAGTTTTGACGNCTTATGGAGGTACTTGTCTTAAGTCTAGATTTGGGGTNTTCAGAAAATTGCTGTTTTTCTTTTCTGAAGCAGGCTACATGAATGATTGGAGTAGATAAAAAATCACTNTCTGCACAATNTTTTTTGTAAATTAATTAATGTATGGAGCCATTCTAATGATAGATAGGGAAATTATTTCCACTGTAACTTGAAGAACAAAAGCTGCCGTATTTTAAGTTAAAGAGGTCTAAAATT
SEQUENCE_INCLUDED_REGION=31,270
P3_COMMENT=CL is TCTTGCCTCTGAAGTTTTGA
P3_COMMENT=CR is GACCTCTTTAACTTAAAATACGGC
P3_COMMENT=CM is ACTTGTCTTAAGTCTAGATTTGGGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.200640
PRIMER_LEFT_0_PENALTY=0.953367
PRIMER_RIGHT_0_PENALTY=2.247273
PRIMER_INTERNAL_0_PENALTY=6.343049
PRIMER_LEFT_0_SEQUENCE=TTGCCTCTGAAGTTTTGACG
PRIMER_RIGHT_0_SEQUENCE=GCCTGCTTCAGAAAAGAAAAA
PRIMER_INTERNAL_0_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
PRIMER_LEFT_0=46,20
PRIMER_RIGHT_0=140,21
PRIMER_INTERNAL_0=79,24
PRIMER_LEFT_0_TM=59.047
PRIMER_RIGHT_0_TM=58.753
PRIMER_INTERNAL_0_TM=57.657
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=3.284990
PRIMER_LEFT_1_PENALTY=1.037717
PRIMER_RIGHT_1_PENALTY=2.247273
PRIMER_INTERNAL_1_PENALTY=6.343049
PRIMER_LEFT_1_SEQUENCE=CTTGCCTCTGAAGTTTTGACG
PRIMER_RIGHT_1_SEQUENCE=GCCTGCTTCAGAAAAGAAAAA
PRIMER_INTERNAL_1_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
PRIMER_LEFT_1=45,21
PRIMER_RIGHT_1=140,21
PRIMER_INTERNAL_1=79,24
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=58.753
PRIMER_INTERNAL_1_TM=57.657
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=3.286596
PRIMER_LEFT_2_PENALTY=0.953367
PRIMER_RIGHT_2_PENALTY=2.333229
PRIMER_INTERNAL_2_PENALTY=6.343049
PRIMER_LEFT_2_SEQUENCE=TTGCCTCTGAAGTTTTGACG
PRIMER_RIGHT_2_SEQUENCE=AGCCTGCTTCAGAAAAGAAAAA
PRIMER_INTERNAL_2_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
PRIMER_LEFT_2=46,20
PRIMER_RIGHT_2=141,22
PRIMER_INTERNAL_2=79,24
PRIMER_LEFT_2_TM=59.047
PRIMER_RIGHT_2_TM=59.667
PRIMER_INTERNAL_2_TM=57.657
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=36.364
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=3.370947
PRIMER_LEFT_3_PENALTY=1.037717
PRIMER_RIGHT_3_PENALTY=2.333229
PRIMER_INTERNAL_3_PENALTY=6.343049
PRIMER_LEFT_3_SEQUENCE=CTTGCCTCTGAAGTTTTGACG
PRIMER_RIGHT_3_SEQUENCE=AGCCTGCTTCAGAAAAGAAAAA
PRIMER_INTERNAL_3_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
PRIMER_LEFT_3=45,21
PRIMER_RIGHT_3=141,22
PRIMER_INTERNAL_3=79,24
PRIMER_LEFT_3_TM=60.038
PRIMER_RIGHT_3_TM=59.667
PRIMER_INTERNAL_3_TM=57.657
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=36.364
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=3.409191
PRIMER_LEFT_4_PENALTY=0.953367
PRIMER_RIGHT_4_PENALTY=2.455824
PRIMER_INTERNAL_4_PENALTY=6.343049
PRIMER_LEFT_4_SEQUENCE=TTGCCTCTGAAGTTTTGACG
PRIMER_RIGHT_4_SEQUENCE=GCCTGCTTCAGAAAAGAAAAAC
PRIMER_INTERNAL_4_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG
PRIMER_LEFT_4=46,20
PRIMER_RIGHT_4=140,22
PRIMER_INTERNAL_4=79,24
PRIMER_LEFT_4_TM=59.047
PRIMER_RIGHT_4_TM=59.544
PRIMER_INTERNAL_4_TM=57.657
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH348
SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCACACTGATACATCCAATTCAAATTTAACACCATTGGGTATATTATAATTTTNGCTCTATCCATAGTTCTAACCCTCTTCTCTGACAGTGAGACACCTGCCTTCTATTGTCCTTGACGTATTAACGTATTCGATCAGTCACCCATCTGGAACCAAGGTTTCATTTCTGCTGACCCCTCCCTCCTCACCCTACTTGGGCTCTGACTTCCTTTCCTGGGCTGAACCTTCTCTGTGTGGCTGTCCGCCTTCCTCTGCTTGGGCTCCAATACCCTACATGGGTGTCTGCTCATCCTGCGCAGAGGGGTAC
SEQUENCE_INCLUDED_REGION=31,308
P3_COMMENT=CL is TTCAAATTTAACACCATTGGG
P3_COMMENT=CR is CACCCATGTAGGGTATTGGA
P3_COMMENT=CM is GCTCTATCCATAGTTCTAACCCTCT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.302917
PRIMER_LEFT_0_PENALTY=0.139259
PRIMER_RIGHT_0_PENALTY=0.163658
PRIMER_INTERNAL_0_PENALTY=0.550349
PRIMER_LEFT_0_SEQUENCE=CTCACCCTACTTGGGCTCTG
PRIMER_RIGHT_0_SEQUENCE=GTATTGGAGCCCAAGCAGAG
PRIMER_INTERNAL_0_SEQUENCE=CTGGGCTGAACCTTCTCTGT
PRIMER_LEFT_0=215,20
PRIMER_RIGHT_0=300,20
PRIMER_INTERNAL_0=245,20
PRIMER_LEFT_0_TM=59.861
PRIMER_RIGHT_0_TM=59.836
PRIMER_INTERNAL_0_TM=59.450
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=10.00
PRIMER_PAIR_0_COMPL_END=8.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=0.442505
PRIMER_LEFT_1_PENALTY=0.278847
PRIMER_RIGHT_1_PENALTY=0.163658
PRIMER_INTERNAL_1_PENALTY=0.550349
PRIMER_LEFT_1_SEQUENCE=CACCCTACTTGGGCTCTGAC
PRIMER_RIGHT_1_SEQUENCE=GTATTGGAGCCCAAGCAGAG
PRIMER_INTERNAL_1_SEQUENCE=CTGGGCTGAACCTTCTCTGT
PRIMER_LEFT_1=217,20
PRIMER_RIGHT_1=300,20
PRIMER_INTERNAL_1=245,20
PRIMER_LEFT_1_TM=59.721
PRIMER_RIGHT_1_TM=59.836
PRIMER_INTERNAL_1_TM=59.450
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=10.00
PRIMER_PAIR_1_COMPL_END=8.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=0.461882
PRIMER_LEFT_2_PENALTY=0.139259
PRIMER_RIGHT_2_PENALTY=0.322623
PRIMER_INTERNAL_2_PENALTY=0.550349
PRIMER_LEFT_2_SEQUENCE=CTCACCCTACTTGGGCTCTG
PRIMER_RIGHT_2_SEQUENCE=TGTAGGGTATTGGAGCCCAA
PRIMER_INTERNAL_2_SEQUENCE=CTGGGCTGAACCTTCTCTGT
PRIMER_LEFT_2=215,20
PRIMER_RIGHT_2=306,20
PRIMER_INTERNAL_2=245,20
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=60.323
PRIMER_INTERNAL_2_TM=59.450
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=0.481624
PRIMER_LEFT_3_PENALTY=0.182682
PRIMER_RIGHT_3_PENALTY=0.298942
PRIMER_INTERNAL_3_PENALTY=0.163658
PRIMER_LEFT_3_SEQUENCE=GGGCTCTGACTTCCTTTCCT
PRIMER_RIGHT_3_SEQUENCE=TGAGCAGACACCCATGTAGG
PRIMER_INTERNAL_3_SEQUENCE=CTCTGCTTGGGCTCCAATAC
PRIMER_LEFT_3=227,20
PRIMER_RIGHT_3=320,20
PRIMER_INTERNAL_3=281,20
PRIMER_LEFT_3_TM=59.817
PRIMER_RIGHT_3_TM=59.701
PRIMER_INTERNAL_3_TM=59.836
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=0.505306
PRIMER_LEFT_4_PENALTY=0.182682
PRIMER_RIGHT_4_PENALTY=0.322623
PRIMER_INTERNAL_4_PENALTY=0.695412
PRIMER_LEFT_4_SEQUENCE=GGGCTCTGACTTCCTTTCCT
PRIMER_RIGHT_4_SEQUENCE=TGTAGGGTATTGGAGCCCAA
PRIMER_INTERNAL_4_SEQUENCE=GGGCTGAACCTTCTCTGTGT
PRIMER_LEFT_4=227,20
PRIMER_RIGHT_4=306,20
PRIMER_INTERNAL_4=247,20
PRIMER_LEFT_4_TM=59.817
PRIMER_RIGHT_4_TM=60.323
PRIMER_INTERNAL_4_TM=59.305
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH354
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTGAATTTAAAAGTNGCCAGTTATTCAGAACCTTGAAAGCACCGTGCAGACCAAGCCAAGGCGCATCCGGTGTGTGCGTCCTCTCCGGTTGGTGGTCTCACTGAGGATGAGGCTGGGGTTTGGATCTTGTGGAGGCAGAGCTGTGTCCTGAGCACAAGAAGGCATGGGATGGGAAAGGCCATTTTCAGCAGCATTTGATGGCATCGAGGTTAGCCAGGCCAGACGTAGAGGGCACTCCCTTTGGGGTCCCCCTTGTTACTGGGGCCCTCT
SEQUENCE_INCLUDED_REGION=36,272
P3_COMMENT=CL is CAGTTATTCAGAACCTTGAAAGC
P3_COMMENT=CR is AGGGCCCCAGTAACAAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.289133
PRIMER_LEFT_0_PENALTY=0.187005
PRIMER_RIGHT_0_PENALTY=0.102129
PRIMER_INTERNAL_0_PENALTY=0.190632
PRIMER_LEFT_0_SEQUENCE=GCTGTGTCCTGAGCACAAGA
PRIMER_RIGHT_0_SEQUENCE=CTACGTCTGGCCTGGCTAAC
PRIMER_INTERNAL_0_SEQUENCE=TTTTCAGCAGCATTTGATGG
PRIMER_LEFT_0=177,20
PRIMER_RIGHT_0=265,20
PRIMER_INTERNAL_0=219,20
PRIMER_LEFT_0_TM=60.187
PRIMER_RIGHT_0_TM=59.898
PRIMER_INTERNAL_0_TM=59.809
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=0.289133
PRIMER_LEFT_1_PENALTY=0.187005
PRIMER_RIGHT_1_PENALTY=0.102129
PRIMER_INTERNAL_1_PENALTY=0.190632
PRIMER_LEFT_1_SEQUENCE=GAGCTGTGTCCTGAGCACAA
PRIMER_RIGHT_1_SEQUENCE=CTACGTCTGGCCTGGCTAAC
PRIMER_INTERNAL_1_SEQUENCE=TTTTCAGCAGCATTTGATGG
PRIMER_LEFT_1=175,20
PRIMER_RIGHT_1=265,20
PRIMER_INTERNAL_1=219,20
PRIMER_LEFT_1_TM=60.187
PRIMER_RIGHT_1_TM=59.898
PRIMER_INTERNAL_1_TM=59.809
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.338502
PRIMER_LEFT_2_PENALTY=0.236374
PRIMER_RIGHT_2_PENALTY=0.102129
PRIMER_INTERNAL_2_PENALTY=0.190632
PRIMER_LEFT_2_SEQUENCE=AGAGCTGTGTCCTGAGCACA
PRIMER_RIGHT_2_SEQUENCE=CTACGTCTGGCCTGGCTAAC
PRIMER_INTERNAL_2_SEQUENCE=TTTTCAGCAGCATTTGATGG
PRIMER_LEFT_2=174,20
PRIMER_RIGHT_2=265,20
PRIMER_INTERNAL_2=219,20
PRIMER_LEFT_2_TM=59.764
PRIMER_RIGHT_2_TM=59.898
PRIMER_INTERNAL_2_TM=59.809
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=0.338502
PRIMER_LEFT_3_PENALTY=0.236374
PRIMER_RIGHT_3_PENALTY=0.102129
PRIMER_INTERNAL_3_PENALTY=0.190632
PRIMER_LEFT_3_SEQUENCE=CAGAGCTGTGTCCTGAGCAC
PRIMER_RIGHT_3_SEQUENCE=CTACGTCTGGCCTGGCTAAC
PRIMER_INTERNAL_3_SEQUENCE=TTTTCAGCAGCATTTGATGG
PRIMER_LEFT_3=173,20
PRIMER_RIGHT_3=265,20
PRIMER_INTERNAL_3=219,20
PRIMER_LEFT_3_TM=59.764
PRIMER_RIGHT_3_TM=59.898
PRIMER_INTERNAL_3_TM=59.809
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=93
PRIMER_PAIR_4_PENALTY=0.491819
PRIMER_LEFT_4_PENALTY=0.290589
PRIMER_RIGHT_4_PENALTY=0.201230
PRIMER_INTERNAL_4_PENALTY=2.261603
PRIMER_LEFT_4_SEQUENCE=AGAACCTTGAAAGCACCGTG
PRIMER_RIGHT_4_SEQUENCE=GCCTCATCCTCAGTGAGACC
PRIMER_INTERNAL_4_SEQUENCE=GGTGTGTGCGTCCTCTCC
PRIMER_LEFT_4=64,20
PRIMER_RIGHT_4=150,20
PRIMER_INTERNAL_4=106,18
PRIMER_LEFT_4_TM=60.291
PRIMER_RIGHT_4_TM=59.799
PRIMER_INTERNAL_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH356
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAATAATAAGGAATTACTTAGTCAAGATAGTTCTTGATCGGTAAATTTTGTCTCACGTACATAGCAGTATATAAAATTCAATTTTTATTTTAGTTAAACTAAGTNTCAAATGACCACAGTGACGATTCCAGCACTGGATCCTGTATTCCCTGGTATTCTATTAGGTCATATTTCAACTACTGTAAATATGTAAATTCTGTTACACAGAAGATCTGGAAATAACCAAATATTCTTTTAAAAGTCAAAACTGTTAGCACTTCAAACCCATTTACTAAAAACTAGAGT
SEQUENCE_INCLUDED_REGION=36,288
P3_COMMENT=CL is TTAGTCAAGATAGTTCTTGATCGG
P3_COMMENT=CR is TTTAGTAAATGGGTTTGAAGTGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.508092
PRIMER_LEFT_0_PENALTY=0.759154
PRIMER_RIGHT_0_PENALTY=4.748938
PRIMER_INTERNAL_0_PENALTY=6.732607
PRIMER_LEFT_0_SEQUENCE=TTCCAGCACTGGATCCTGTA
PRIMER_RIGHT_0_SEQUENCE=TGGTTATTTCCAGATCTTCTGTG
PRIMER_INTERNAL_0_SEQUENCE=TTCCCTGGTATTCTATTAGGTCAT
PRIMER_LEFT_0=164,20
PRIMER_RIGHT_0=263,23
PRIMER_INTERNAL_0=184,24
PRIMER_LEFT_0_TM=59.241
PRIMER_RIGHT_0_TM=58.251
PRIMER_INTERNAL_0_TM=57.267
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_INTERNAL_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=5.690360
PRIMER_LEFT_1_PENALTY=0.759154
PRIMER_RIGHT_1_PENALTY=4.931205
PRIMER_INTERNAL_1_PENALTY=6.732607
PRIMER_LEFT_1_SEQUENCE=TTCCAGCACTGGATCCTGTA
PRIMER_RIGHT_1_SEQUENCE=TGGTTATTTCCAGATCTTCTGTGT
PRIMER_INTERNAL_1_SEQUENCE=TTCCCTGGTATTCTATTAGGTCAT
PRIMER_LEFT_1=164,20
PRIMER_RIGHT_1=263,24
PRIMER_INTERNAL_1=184,24
PRIMER_LEFT_1_TM=59.241
PRIMER_RIGHT_1_TM=59.069
PRIMER_INTERNAL_1_TM=57.267
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=37.500
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=5.895779
PRIMER_LEFT_2_PENALTY=1.435246
PRIMER_RIGHT_2_PENALTY=4.460533
PRIMER_INTERNAL_2_PENALTY=8.821726
PRIMER_LEFT_2_SEQUENCE=CCAGCACTGGATCCTGTATTC
PRIMER_RIGHT_2_SEQUENCE=TTGGTTATTTCCAGATCTTCTGTG
PRIMER_INTERNAL_2_SEQUENCE=CCTGGTATTCTATTAGGTCATATTTCA
PRIMER_LEFT_2=166,21
PRIMER_RIGHT_2=264,24
PRIMER_INTERNAL_2=187,27
PRIMER_LEFT_2_TM=59.565
PRIMER_RIGHT_2_TM=59.539
PRIMER_INTERNAL_2_TM=58.178
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=37.500
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=5.895779
PRIMER_LEFT_3_PENALTY=1.435246
PRIMER_RIGHT_3_PENALTY=4.460533
PRIMER_INTERNAL_3_PENALTY=8.188024
PRIMER_LEFT_3_SEQUENCE=TCCAGCACTGGATCCTGTATT
PRIMER_RIGHT_3_SEQUENCE=TTGGTTATTTCCAGATCTTCTGTG
PRIMER_INTERNAL_3_SEQUENCE=CCCTGGTATTCTATTAGGTCATATTTC
PRIMER_LEFT_3=165,21
PRIMER_RIGHT_3=264,24
PRIMER_INTERNAL_3=186,27
PRIMER_LEFT_3_TM=59.565
PRIMER_RIGHT_3_TM=59.539
PRIMER_INTERNAL_3_TM=58.812
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=37.500
PRIMER_INTERNAL_3_GC_PERCENT=37.037
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=6.184184
PRIMER_LEFT_4_PENALTY=1.435246
PRIMER_RIGHT_4_PENALTY=4.748938
PRIMER_INTERNAL_4_PENALTY=8.821726
PRIMER_LEFT_4_SEQUENCE=CCAGCACTGGATCCTGTATTC
PRIMER_RIGHT_4_SEQUENCE=TGGTTATTTCCAGATCTTCTGTG
PRIMER_INTERNAL_4_SEQUENCE=CCTGGTATTCTATTAGGTCATATTTCA
PRIMER_LEFT_4=166,21
PRIMER_RIGHT_4=263,23
PRIMER_INTERNAL_4=187,27
PRIMER_LEFT_4_TM=59.565
PRIMER_RIGHT_4_TM=58.251
PRIMER_INTERNAL_4_TM=58.178
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH362
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGGCTTTGGAACTAAGCCTGAAGGTCAAATAGGGTATCCATAGCAGGGGTCTGGGGCACTAGACAGTCTGTTCATTTTCAGGAATATATGGGTCANTTTTGTATGTGTGACCT
SEQUENCE_INCLUDED_REGION=33,113
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.681940
PRIMER_LEFT_0_PENALTY=0.377925
PRIMER_RIGHT_0_PENALTY=3.304014
PRIMER_INTERNAL_0_PENALTY=0.326207
PRIMER_LEFT_0_SEQUENCE=TGGCTTTGGAACTAAGCCTG
PRIMER_RIGHT_0_SEQUENCE=AATGAACAGACTGTCTAGTGCCC
PRIMER_INTERNAL_0_SEQUENCE=AGGGTATCCATAGCAGGGGT
PRIMER_LEFT_0=33,20
PRIMER_RIGHT_0=108,23
PRIMER_INTERNAL_0=63,20
PRIMER_LEFT_0_TM=60.378
PRIMER_RIGHT_0_TM=59.696
PRIMER_INTERNAL_0_TM=59.674
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.826
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=3.851522
PRIMER_LEFT_1_PENALTY=0.547508
PRIMER_RIGHT_1_PENALTY=3.304014
PRIMER_INTERNAL_1_PENALTY=0.326207
PRIMER_LEFT_1_SEQUENCE=GGCTTTGGAACTAAGCCTGA
PRIMER_RIGHT_1_SEQUENCE=AATGAACAGACTGTCTAGTGCCC
PRIMER_INTERNAL_1_SEQUENCE=AGGGTATCCATAGCAGGGGT
PRIMER_LEFT_1=34,20
PRIMER_RIGHT_1=108,23
PRIMER_INTERNAL_1=63,20
PRIMER_LEFT_1_TM=59.452
PRIMER_RIGHT_1_TM=59.696
PRIMER_INTERNAL_1_TM=59.674
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=4.042376
PRIMER_LEFT_2_PENALTY=0.377925
PRIMER_RIGHT_2_PENALTY=3.664451
PRIMER_INTERNAL_2_PENALTY=0.326207
PRIMER_LEFT_2_SEQUENCE=TGGCTTTGGAACTAAGCCTG
PRIMER_RIGHT_2_SEQUENCE=ATGAACAGACTGTCTAGTGCCC
PRIMER_INTERNAL_2_SEQUENCE=AGGGTATCCATAGCAGGGGT
PRIMER_LEFT_2=33,20
PRIMER_RIGHT_2=107,22
PRIMER_INTERNAL_2=63,20
PRIMER_LEFT_2_TM=60.378
PRIMER_RIGHT_2_TM=58.336
PRIMER_INTERNAL_2_TM=59.674
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=4.790071
PRIMER_LEFT_3_PENALTY=0.377925
PRIMER_RIGHT_3_PENALTY=4.412146
PRIMER_INTERNAL_3_PENALTY=0.090155
PRIMER_LEFT_3_SEQUENCE=TGGCTTTGGAACTAAGCCTG
PRIMER_RIGHT_3_SEQUENCE=TGACCCATATATTCCTGAAAATGA
PRIMER_INTERNAL_3_SEQUENCE=TATCCATAGCAGGGGTCTGG
PRIMER_LEFT_3=33,20
PRIMER_RIGHT_3=127,24
PRIMER_INTERNAL_3=67,20
PRIMER_LEFT_3_TM=60.378
PRIMER_RIGHT_3_TM=59.588
PRIMER_INTERNAL_3_TM=59.910
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=4.959654
PRIMER_LEFT_4_PENALTY=0.547508
PRIMER_RIGHT_4_PENALTY=4.412146
PRIMER_INTERNAL_4_PENALTY=0.090155
PRIMER_LEFT_4_SEQUENCE=GGCTTTGGAACTAAGCCTGA
PRIMER_RIGHT_4_SEQUENCE=TGACCCATATATTCCTGAAAATGA
PRIMER_INTERNAL_4_SEQUENCE=TATCCATAGCAGGGGTCTGG
PRIMER_LEFT_4=34,20
PRIMER_RIGHT_4=127,24
PRIMER_INTERNAL_4=67,20
PRIMER_LEFT_4_TM=59.452
PRIMER_RIGHT_4_TM=59.588
PRIMER_INTERNAL_4_TM=59.910
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=33.333
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=94
=
SEQUENCE_ID=MH364
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGGCTTCCTTTCTCTACAAGGAGGAAATTGAAACCATTTGTGAAAGAGCATTTCCTTTTCAGAGTCTTATCCTAGTACCTCCGAGAACTCTGTGAGTCTGTTCATAGACGGGGAGGGAGCAGGAGAAAAGGCTGAGGGCTTTTTAATACCTGGGNCAAAAGAATCTTTCCCTTAAAATAAAAAATGC
SEQUENCE_INCLUDED_REGION=36,190
P3_COMMENT=CL is TAGGCTTCCTTTCTCTACAAGG
P3_COMMENT=CR is CCCAGGTATTAAAAAGCCCT
P3_COMMENT=CM is GGAAATTGAAACCATTTGTGA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.421963
PRIMER_LEFT_0_PENALTY=2.356567
PRIMER_RIGHT_0_PENALTY=0.065396
PRIMER_INTERNAL_0_PENALTY=3.234797
PRIMER_LEFT_0_SEQUENCE=CAAGGAGGAAATTGAAACCA
PRIMER_RIGHT_0_SEQUENCE=CCTCCCCGTCTATGAACAGA
PRIMER_INTERNAL_0_SEQUENCE=TTGTGAAAGAGCATTTCCTTTTC
PRIMER_LEFT_0=55,20
PRIMER_RIGHT_0=154,20
PRIMER_INTERNAL_0=76,23
PRIMER_LEFT_0_TM=57.643
PRIMER_RIGHT_0_TM=60.065
PRIMER_INTERNAL_0_TM=59.765
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=2.660388
PRIMER_LEFT_1_PENALTY=2.594992
PRIMER_RIGHT_1_PENALTY=0.065396
PRIMER_INTERNAL_1_PENALTY=3.349942
PRIMER_LEFT_1_SEQUENCE=GGAGGAAATTGAAACCATTTG
PRIMER_RIGHT_1_SEQUENCE=CCTCCCCGTCTATGAACAGA
PRIMER_INTERNAL_1_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA
PRIMER_LEFT_1=58,21
PRIMER_RIGHT_1=154,20
PRIMER_INTERNAL_1=79,23
PRIMER_LEFT_1_TM=58.405
PRIMER_RIGHT_1_TM=60.065
PRIMER_INTERNAL_1_TM=60.350
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=2.713818
PRIMER_LEFT_2_PENALTY=2.648422
PRIMER_RIGHT_2_PENALTY=0.065396
PRIMER_INTERNAL_2_PENALTY=3.882472
PRIMER_LEFT_2_SEQUENCE=TGAAACCATTTGTGAAAGAGCA
PRIMER_RIGHT_2_SEQUENCE=CCTCCCCGTCTATGAACAGA
PRIMER_INTERNAL_2_SEQUENCE=TACCTCCGAGAACTCTGTGAG
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=154,20
PRIMER_INTERNAL_2=114,21
PRIMER_LEFT_2_TM=60.648
PRIMER_RIGHT_2_TM=60.065
PRIMER_INTERNAL_2_TM=57.118
PRIMER_LEFT_2_GC_PERCENT=36.364
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=2.714943
PRIMER_LEFT_3_PENALTY=2.649547
PRIMER_RIGHT_3_PENALTY=0.065396
PRIMER_INTERNAL_3_PENALTY=3.349942
PRIMER_LEFT_3_SEQUENCE=AGGAGGAAATTGAAACCATTTG
PRIMER_RIGHT_3_SEQUENCE=CCTCCCCGTCTATGAACAGA
PRIMER_INTERNAL_3_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=154,20
PRIMER_INTERNAL_3=79,23
PRIMER_LEFT_3_TM=59.350
PRIMER_RIGHT_3_TM=60.065
PRIMER_INTERNAL_3_TM=60.350
PRIMER_LEFT_3_GC_PERCENT=36.364
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=2.714943
PRIMER_LEFT_4_PENALTY=2.649547
PRIMER_RIGHT_4_PENALTY=0.065396
PRIMER_INTERNAL_4_PENALTY=3.349942
PRIMER_LEFT_4_SEQUENCE=CAAGGAGGAAATTGAAACCATT
PRIMER_RIGHT_4_SEQUENCE=CCTCCCCGTCTATGAACAGA
PRIMER_INTERNAL_4_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=154,20
PRIMER_INTERNAL_4=79,23
PRIMER_LEFT_4_TM=59.350
PRIMER_RIGHT_4_TM=60.065
PRIMER_INTERNAL_4_TM=60.350
PRIMER_LEFT_4_GC_PERCENT=36.364
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH367
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTCGGGAGGCTGAGGCAGGAGAATGGCCAGAACCTAGGAGGCGGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTT
SEQUENCE_INCLUDED_REGION=36,49
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH373
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTATTCTCTCTCTGTTTCACTCTCACTTCCCCTTTCTCTGCCTTGAATGCCTATNATATATTGGCTTTACTTGGATTTCCTCTTTAAATTGAAGGTGAATATAATGTCTTTACTTTTTGGGTTATTGGCTCTGTAGATGTTCCTCTTCAGTTGCAAGGGACAGAAACCAATCTATTTTTCCTGACAACCTCTGAAATAAAAGTATGGAAAAGTTAAATGGCTTTAATATAAG
SEQUENCE_INCLUDED_REGION=34,235
P3_COMMENT=CL is TGTTTCACTCTCACTTCCCC
P3_COMMENT=CR is GTTTCTGTCCCTTGCAACTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.757357
PRIMER_LEFT_0_PENALTY=2.390906
PRIMER_RIGHT_0_PENALTY=0.366450
PRIMER_INTERNAL_0_PENALTY=2.244591
PRIMER_LEFT_0_SEQUENCE=TTGGCTTTACTTGGATTTCCTC
PRIMER_RIGHT_0_SEQUENCE=CCCTTGCAACTGAAGAGGAA
PRIMER_INTERNAL_0_SEQUENCE=TTTTTGGGTTATTGGCTCTG
PRIMER_LEFT_0=97,22
PRIMER_RIGHT_0=195,20
PRIMER_INTERNAL_0=150,20
PRIMER_LEFT_0_TM=59.609
PRIMER_RIGHT_0_TM=60.366
PRIMER_INTERNAL_0_TM=57.755
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=2.840700
PRIMER_LEFT_1_PENALTY=2.684639
PRIMER_RIGHT_1_PENALTY=0.156061
PRIMER_INTERNAL_1_PENALTY=2.244591
PRIMER_LEFT_1_SEQUENCE=TGGCTTTACTTGGATTTCCTC
PRIMER_RIGHT_1_SEQUENCE=GTCCCTTGCAACTGAAGAGG
PRIMER_INTERNAL_1_SEQUENCE=TTTTTGGGTTATTGGCTCTG
PRIMER_LEFT_1=98,21
PRIMER_RIGHT_1=197,20
PRIMER_INTERNAL_1=150,20
PRIMER_LEFT_1_TM=58.315
PRIMER_RIGHT_1_TM=59.844
PRIMER_INTERNAL_1_TM=57.755
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=2.892165
PRIMER_LEFT_2_PENALTY=2.736104
PRIMER_RIGHT_2_PENALTY=0.156061
PRIMER_INTERNAL_2_PENALTY=2.244591
PRIMER_LEFT_2_SEQUENCE=TGGCTTTACTTGGATTTCCTCT
PRIMER_RIGHT_2_SEQUENCE=GTCCCTTGCAACTGAAGAGG
PRIMER_INTERNAL_2_SEQUENCE=TTTTTGGGTTATTGGCTCTG
PRIMER_LEFT_2=98,22
PRIMER_RIGHT_2=197,20
PRIMER_INTERNAL_2=150,20
PRIMER_LEFT_2_TM=59.264
PRIMER_RIGHT_2_TM=59.844
PRIMER_INTERNAL_2_TM=57.755
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=3.051089
PRIMER_LEFT_3_PENALTY=2.684639
PRIMER_RIGHT_3_PENALTY=0.366450
PRIMER_INTERNAL_3_PENALTY=2.244591
PRIMER_LEFT_3_SEQUENCE=TGGCTTTACTTGGATTTCCTC
PRIMER_RIGHT_3_SEQUENCE=CCCTTGCAACTGAAGAGGAA
PRIMER_INTERNAL_3_SEQUENCE=TTTTTGGGTTATTGGCTCTG
PRIMER_LEFT_3=98,21
PRIMER_RIGHT_3=195,20
PRIMER_INTERNAL_3=150,20
PRIMER_LEFT_3_TM=58.315
PRIMER_RIGHT_3_TM=60.366
PRIMER_INTERNAL_3_TM=57.755
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=3.102554
PRIMER_LEFT_4_PENALTY=2.736104
PRIMER_RIGHT_4_PENALTY=0.366450
PRIMER_INTERNAL_4_PENALTY=2.244591
PRIMER_LEFT_4_SEQUENCE=TGGCTTTACTTGGATTTCCTCT
PRIMER_RIGHT_4_SEQUENCE=CCCTTGCAACTGAAGAGGAA
PRIMER_INTERNAL_4_SEQUENCE=TTTTTGGGTTATTGGCTCTG
PRIMER_LEFT_4=98,22
PRIMER_RIGHT_4=195,20
PRIMER_INTERNAL_4=150,20
PRIMER_LEFT_4_TM=59.264
PRIMER_RIGHT_4_TM=60.366
PRIMER_INTERNAL_4_TM=57.755
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=7.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH375
SEQUENCE_TEMPLATE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTAGCATTTAGACCTGGAAGCCTTTCTGCTACCCAAAGTGCCACGTGTTGGGTTGAAGCAGGCTGCCTCAGGACAACCAGTCACTCGGAGCACTTGCCTCCATATCCAGGCTAACCAGTGTGTTGGTATAAGTGGTTTTCAAGTAGGG
SEQUENCE_INCLUDED_REGION=31,151
P3_COMMENT=CL is CTTCTAGCATTTAGACCTGGA
P3_COMMENT=CR is CCTACTTGAAAACCACTTATACC
P3_COMMENT=CM is GCCTTTCTGCTACCCAAAGT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.590422
PRIMER_LEFT_0_PENALTY=0.248900
PRIMER_RIGHT_0_PENALTY=0.341522
PRIMER_INTERNAL_0_PENALTY=0.723365
PRIMER_LEFT_0_SEQUENCE=TTTCTGCTACCCAAAGTGCC
PRIMER_RIGHT_0_SEQUENCE=GATATGGAGGCAAGTGCTCC
PRIMER_INTERNAL_0_SEQUENCE=GCCTCAGGACAACCAGTCAC
PRIMER_LEFT_0=56,20
PRIMER_RIGHT_0=139,20
PRIMER_INTERNAL_0=98,20
PRIMER_LEFT_0_TM=60.249
PRIMER_RIGHT_0_TM=59.658
PRIMER_INTERNAL_0_TM=60.723
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=0.590422
PRIMER_LEFT_1_PENALTY=0.248900
PRIMER_RIGHT_1_PENALTY=0.341522
PRIMER_INTERNAL_1_PENALTY=0.135808
PRIMER_LEFT_1_SEQUENCE=TTTCTGCTACCCAAAGTGCC
PRIMER_RIGHT_1_SEQUENCE=GGATATGGAGGCAAGTGCTC
PRIMER_INTERNAL_1_SEQUENCE=TCAGGACAACCAGTCACTCG
PRIMER_LEFT_1=56,20
PRIMER_RIGHT_1=140,20
PRIMER_INTERNAL_1=101,20
PRIMER_LEFT_1_TM=60.249
PRIMER_RIGHT_1_TM=59.658
PRIMER_INTERNAL_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=0.640168
PRIMER_LEFT_2_PENALTY=0.547508
PRIMER_RIGHT_2_PENALTY=0.092660
PRIMER_INTERNAL_2_PENALTY=1.264463
PRIMER_LEFT_2_SEQUENCE=GAAGCCTTTCTGCTACCCAA
PRIMER_RIGHT_2_SEQUENCE=AGTGCTCCGAGTGACTGGTT
PRIMER_INTERNAL_2_SEQUENCE=ACGTGTTGGGTTGAAGCAG
PRIMER_LEFT_2=50,20
PRIMER_RIGHT_2=127,20
PRIMER_INTERNAL_2=76,19
PRIMER_LEFT_2_TM=59.452
PRIMER_RIGHT_2_TM=59.907
PRIMER_INTERNAL_2_TM=59.736
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=52.632
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=0.640168
PRIMER_LEFT_3_PENALTY=0.547508
PRIMER_RIGHT_3_PENALTY=0.092660
PRIMER_INTERNAL_3_PENALTY=1.264463
PRIMER_LEFT_3_SEQUENCE=GAAGCCTTTCTGCTACCCAA
PRIMER_RIGHT_3_SEQUENCE=AAGTGCTCCGAGTGACTGGT
PRIMER_INTERNAL_3_SEQUENCE=ACGTGTTGGGTTGAAGCAG
PRIMER_LEFT_3=50,20
PRIMER_RIGHT_3=128,20
PRIMER_INTERNAL_3=76,19
PRIMER_LEFT_3_TM=59.452
PRIMER_RIGHT_3_TM=59.907
PRIMER_INTERNAL_3_TM=59.736
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=52.632
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=0.722452
PRIMER_LEFT_4_PENALTY=0.248900
PRIMER_RIGHT_4_PENALTY=0.473552
PRIMER_INTERNAL_4_PENALTY=0.135808
PRIMER_LEFT_4_SEQUENCE=TTTCTGCTACCCAAAGTGCC
PRIMER_RIGHT_4_SEQUENCE=CCTGGATATGGAGGCAAGTG
PRIMER_INTERNAL_4_SEQUENCE=TCAGGACAACCAGTCACTCG
PRIMER_LEFT_4=56,20
PRIMER_RIGHT_4=143,20
PRIMER_INTERNAL_4=101,20
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=60.474
PRIMER_INTERNAL_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH378
SEQUENCE_TEMPLATE=CATCTGGGATTTTAGTTTTTACTATTAATATTTGTGGGAACATTTCCGTCATTAAAAACTCCACATCATCATACCATATGGTGTAATAATATCCGCTTGTNTGGGCATACCATGACTTAACTGCTCTCCTATTGTTACATGTTTAGAATGTTTCCATTTTTTCACAATTAAAAATGGTGCCATCTGTGCTCATATCCATGATTATTTCCTTAAGTAAGTTCCAGAAGTGGAATTGTTGTATCAAGGGATTTGACAAATTCTAAAGCTTTCG
SEQUENCE_INCLUDED_REGION=0,271
P3_COMMENT=CL is CATCTGGGATTTTAGTTTTTACTAT
P3_COMMENT=CR is AATCCCTTGATACAACAATTCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.464599
PRIMER_LEFT_0_PENALTY=0.347318
PRIMER_RIGHT_0_PENALTY=2.117281
PRIMER_INTERNAL_0_PENALTY=3.162827
PRIMER_LEFT_0_SEQUENCE=GTGGGAACATTTCCGTCATT
PRIMER_RIGHT_0_SEQUENCE=CAGTTAAGTCATGGTATGCCCA
PRIMER_INTERNAL_0_SEQUENCE=CCACATCATCATACCATATGGTG
PRIMER_LEFT_0=33,20
PRIMER_RIGHT_0=122,22
PRIMER_INTERNAL_0=60,23
PRIMER_LEFT_0_TM=59.653
PRIMER_RIGHT_0_TM=59.883
PRIMER_INTERNAL_0_TM=59.837
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=2.481949
PRIMER_LEFT_1_PENALTY=2.117281
PRIMER_RIGHT_1_PENALTY=0.364668
PRIMER_INTERNAL_1_PENALTY=5.273133
PRIMER_LEFT_1_SEQUENCE=TGGGCATACCATGACTTAACTG
PRIMER_RIGHT_1_SEQUENCE=GGATATGAGCACAGATGGCA
PRIMER_INTERNAL_1_SEQUENCE=AGAATGTTTCCATTTTTTCACAA
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=196,20
PRIMER_INTERNAL_1=144,23
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=59.635
PRIMER_INTERNAL_1_TM=57.727
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=26.087
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=2.481949
PRIMER_LEFT_2_PENALTY=2.117281
PRIMER_RIGHT_2_PENALTY=0.364668
PRIMER_INTERNAL_2_PENALTY=5.273133
PRIMER_LEFT_2_SEQUENCE=TGGGCATACCATGACTTAACTG
PRIMER_RIGHT_2_SEQUENCE=TGGATATGAGCACAGATGGC
PRIMER_INTERNAL_2_SEQUENCE=AGAATGTTTCCATTTTTTCACAA
PRIMER_LEFT_2=101,22
PRIMER_RIGHT_2=197,20
PRIMER_INTERNAL_2=144,23
PRIMER_LEFT_2_TM=59.883
PRIMER_RIGHT_2_TM=59.635
PRIMER_INTERNAL_2_TM=57.727
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=26.087
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=2.643199
PRIMER_LEFT_3_PENALTY=2.117281
PRIMER_RIGHT_3_PENALTY=0.525918
PRIMER_INTERNAL_3_PENALTY=5.273133
PRIMER_LEFT_3_SEQUENCE=TGGGCATACCATGACTTAACTG
PRIMER_RIGHT_3_SEQUENCE=AGCACAGATGGCACCATTTT
PRIMER_INTERNAL_3_SEQUENCE=AGAATGTTTCCATTTTTTCACAA
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=189,20
PRIMER_INTERNAL_3=144,23
PRIMER_LEFT_3_TM=59.883
PRIMER_RIGHT_3_TM=60.526
PRIMER_INTERNAL_3_TM=57.727
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=26.087
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=2.851445
PRIMER_LEFT_4_PENALTY=0.734164
PRIMER_RIGHT_4_PENALTY=2.117281
PRIMER_INTERNAL_4_PENALTY=3.162827
PRIMER_LEFT_4_SEQUENCE=TTTGTGGGAACATTTCCGTC
PRIMER_RIGHT_4_SEQUENCE=CAGTTAAGTCATGGTATGCCCA
PRIMER_INTERNAL_4_SEQUENCE=CCACATCATCATACCATATGGTG
PRIMER_LEFT_4=30,20
PRIMER_RIGHT_4=122,22
PRIMER_INTERNAL_4=60,23
PRIMER_LEFT_4_TM=60.734
PRIMER_RIGHT_4_TM=59.883
PRIMER_INTERNAL_4_TM=59.837
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH379
SEQUENCE_TEMPLATE=GTTTTGCGGGTTAGAGAGCGGGGCGTGGGGGCGGGGAGGGAGAGTAACAATTTTGGAAGTCCCTGCGTGGTATCTGGAAAGTTAACGACGCTTCAGGTTCTAGGTTGAGCCCAATGACCGTCTTGCCNNGGCAGGGTACCNAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCNCACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
SEQUENCE_INCLUDED_REGION=0,133
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.565856
PRIMER_LEFT_0_PENALTY=0.319419
PRIMER_RIGHT_0_PENALTY=0.246438
PRIMER_INTERNAL_0_PENALTY=0.133741
PRIMER_LEFT_0_SEQUENCE=CGGGGAGGGAGAGTAACAAT
PRIMER_RIGHT_0_SEQUENCE=TGGGCTCAACCTAGAACCTG
PRIMER_INTERNAL_0_SEQUENCE=AAGTCCCTGCGTGGTATCTG
PRIMER_LEFT_0=31,20
PRIMER_RIGHT_0=112,20
PRIMER_INTERNAL_0=56,20
PRIMER_LEFT_0_TM=60.319
PRIMER_RIGHT_0_TM=60.246
PRIMER_INTERNAL_0_TM=60.134
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=0.769248
PRIMER_LEFT_1_PENALTY=0.319419
PRIMER_RIGHT_1_PENALTY=0.449829
PRIMER_INTERNAL_1_PENALTY=0.133741
PRIMER_LEFT_1_SEQUENCE=CGGGGAGGGAGAGTAACAAT
PRIMER_RIGHT_1_SEQUENCE=GGTCATTGGGCTCAACCTAG
PRIMER_INTERNAL_1_SEQUENCE=AAGTCCCTGCGTGGTATCTG
PRIMER_LEFT_1=31,20
PRIMER_RIGHT_1=118,20
PRIMER_INTERNAL_1=56,20
PRIMER_LEFT_1_TM=60.319
PRIMER_RIGHT_1_TM=59.550
PRIMER_INTERNAL_1_TM=60.134
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=1.035545
PRIMER_LEFT_2_PENALTY=0.319419
PRIMER_RIGHT_2_PENALTY=0.716126
PRIMER_INTERNAL_2_PENALTY=0.133741
PRIMER_LEFT_2_SEQUENCE=CGGGGAGGGAGAGTAACAAT
PRIMER_RIGHT_2_SEQUENCE=GGGCTCAACCTAGAACCTGA
PRIMER_INTERNAL_2_SEQUENCE=AAGTCCCTGCGTGGTATCTG
PRIMER_LEFT_2=31,20
PRIMER_RIGHT_2=111,20
PRIMER_INTERNAL_2=56,20
PRIMER_LEFT_2_TM=60.319
PRIMER_RIGHT_2_TM=59.284
PRIMER_INTERNAL_2_TM=60.134
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=1.296602
PRIMER_LEFT_3_PENALTY=1.050165
PRIMER_RIGHT_3_PENALTY=0.246438
PRIMER_INTERNAL_3_PENALTY=0.133741
PRIMER_LEFT_3_SEQUENCE=CGGGGAGGGAGAGTAACAA
PRIMER_RIGHT_3_SEQUENCE=TGGGCTCAACCTAGAACCTG
PRIMER_INTERNAL_3_SEQUENCE=AAGTCCCTGCGTGGTATCTG
PRIMER_LEFT_3=31,19
PRIMER_RIGHT_3=112,20
PRIMER_INTERNAL_3=56,20
PRIMER_LEFT_3_TM=60.050
PRIMER_RIGHT_3_TM=60.246
PRIMER_INTERNAL_3_TM=60.134
PRIMER_LEFT_3_GC_PERCENT=57.895
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=1.499994
PRIMER_LEFT_4_PENALTY=1.050165
PRIMER_RIGHT_4_PENALTY=0.449829
PRIMER_INTERNAL_4_PENALTY=0.133741
PRIMER_LEFT_4_SEQUENCE=CGGGGAGGGAGAGTAACAA
PRIMER_RIGHT_4_SEQUENCE=GGTCATTGGGCTCAACCTAG
PRIMER_INTERNAL_4_SEQUENCE=AAGTCCCTGCGTGGTATCTG
PRIMER_LEFT_4=31,19
PRIMER_RIGHT_4=118,20
PRIMER_INTERNAL_4=56,20
PRIMER_LEFT_4_TM=60.050
PRIMER_RIGHT_4_TM=59.550
PRIMER_INTERNAL_4_TM=60.134
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH385
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTGAACAAATGCAGGGGTGTGGGTTGGAGTGGCGTGTCTCTACTAAAGGCTCCTGGGAAGGAGGGGTACCGAGNTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGNTAACNCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
SEQUENCE_INCLUDED_REGION=35,63
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH396
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTATGAGATTCAGTGTNTTGTCATCTATCCCATCTCATCACCCACCCTTCTCTAACTTGGTTACTCTTTTCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
SEQUENCE_INCLUDED_REGION=35,76
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH399
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATATTACTCCCTATAATTATAACAGACTACCAGACTGGATTTTAGGCTTGTCTGTTCCACTAAAGAACTGGTGACTCTGGGACTAGAATTTGTAAGATCAGTGTAGACTAGGATGGGATTTACAATTCTGGTGCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGA
SEQUENCE_INCLUDED_REGION=36,138
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.405024
PRIMER_LEFT_0_PENALTY=2.219979
PRIMER_RIGHT_0_PENALTY=1.185045
PRIMER_INTERNAL_0_PENALTY=9.644191
PRIMER_LEFT_0_SEQUENCE=AAAGAACTGGTGACTCTGGGA
PRIMER_RIGHT_0_SEQUENCE=TGGCACCAGAATTGTAAATCC
PRIMER_INTERNAL_0_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG
PRIMER_LEFT_0=99,21
PRIMER_RIGHT_0=173,21
PRIMER_INTERNAL_0=126,27
PRIMER_LEFT_0_TM=58.780
PRIMER_RIGHT_0_TM=59.815
PRIMER_INTERNAL_0_TM=57.356
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=37.037
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=3.539926
PRIMER_LEFT_1_PENALTY=2.354881
PRIMER_RIGHT_1_PENALTY=1.185045
PRIMER_INTERNAL_1_PENALTY=9.644191
PRIMER_LEFT_1_SEQUENCE=AAAGAACTGGTGACTCTGGGAC
PRIMER_RIGHT_1_SEQUENCE=TGGCACCAGAATTGTAAATCC
PRIMER_INTERNAL_1_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=173,21
PRIMER_INTERNAL_1=126,27
PRIMER_LEFT_1_TM=59.645
PRIMER_RIGHT_1_TM=59.815
PRIMER_INTERNAL_1_TM=57.356
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_INTERNAL_1_GC_PERCENT=37.037
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=4.048802
PRIMER_LEFT_2_PENALTY=2.863756
PRIMER_RIGHT_2_PENALTY=1.185045
PRIMER_INTERNAL_2_PENALTY=2.219979
PRIMER_LEFT_2_SEQUENCE=GGATTTTAGGCTTGTCTGTTCC
PRIMER_RIGHT_2_SEQUENCE=GCACCAGAATTGTAAATCCCA
PRIMER_INTERNAL_2_SEQUENCE=AAAGAACTGGTGACTCTGGGA
PRIMER_LEFT_2=74,22
PRIMER_RIGHT_2=171,21
PRIMER_INTERNAL_2=99,21
PRIMER_LEFT_2_TM=59.136
PRIMER_RIGHT_2_TM=59.815
PRIMER_INTERNAL_2_TM=58.780
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=98
PRIMER_PAIR_3_PENALTY=4.048802
PRIMER_LEFT_3_PENALTY=2.863756
PRIMER_RIGHT_3_PENALTY=1.185045
PRIMER_INTERNAL_3_PENALTY=2.219979
PRIMER_LEFT_3_SEQUENCE=GGATTTTAGGCTTGTCTGTTCC
PRIMER_RIGHT_3_SEQUENCE=TGGCACCAGAATTGTAAATCC
PRIMER_INTERNAL_3_SEQUENCE=AAAGAACTGGTGACTCTGGGA
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=173,21
PRIMER_INTERNAL_3=99,21
PRIMER_LEFT_3_TM=59.136
PRIMER_RIGHT_3_TM=59.815
PRIMER_INTERNAL_3_TM=58.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=4.347765
PRIMER_LEFT_4_PENALTY=3.162720
PRIMER_RIGHT_4_PENALTY=1.185045
PRIMER_INTERNAL_4_PENALTY=9.644191
PRIMER_LEFT_4_SEQUENCE=TAAAGAACTGGTGACTCTGGGA
PRIMER_RIGHT_4_SEQUENCE=TGGCACCAGAATTGTAAATCC
PRIMER_INTERNAL_4_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG
PRIMER_LEFT_4=98,22
PRIMER_RIGHT_4=173,21
PRIMER_INTERNAL_4=126,27
PRIMER_LEFT_4_TM=58.837
PRIMER_RIGHT_4_TM=59.815
PRIMER_INTERNAL_4_TM=57.356
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=37.037
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH426
SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCGGACATGGTGGTGGGCACTGTAGTCCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
SEQUENCE_INCLUDED_REGION=35,29
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH429
SEQUENCE_TEMPLATE=CTCTATACATATTTAAAATTTTTNTTGTTACAAAGATAACTGTATCAAGGTAGAGGNTAAAACATATTTNATTTAACTTTTTGTTGTCATTGTTGTTGTTGTTTTGAGATGGAGTNTCGCTCTGTCGCCCAGGTTGGAGTGCAGTGGTGTGATCTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
SEQUENCE_INCLUDED_REGION=0,158
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.245683
PRIMER_LEFT_0_PENALTY=4.147950
PRIMER_RIGHT_0_PENALTY=2.097734
PRIMER_INTERNAL_0_PENALTY=4.867800
PRIMER_LEFT_0_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG
PRIMER_RIGHT_0_SEQUENCE=CTGAGATCACACCACTGCACT
PRIMER_INTERNAL_0_SEQUENCE=CTCTGTCGCCCAGGTTGG
PRIMER_LEFT_0=77,24
PRIMER_RIGHT_0=157,21
PRIMER_INTERNAL_0=119,18
PRIMER_LEFT_0_TM=59.852
PRIMER_RIGHT_0_TM=58.902
PRIMER_INTERNAL_0_TM=62.868
PRIMER_LEFT_0_GC_PERCENT=29.167
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_INTERNAL_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=6.428273
PRIMER_LEFT_1_PENALTY=4.330540
PRIMER_RIGHT_1_PENALTY=2.097734
PRIMER_INTERNAL_1_PENALTY=4.867800
PRIMER_LEFT_1_SEQUENCE=TTGTCATTGTTGTTGTTGTTTTGA
PRIMER_RIGHT_1_SEQUENCE=CTGAGATCACACCACTGCACT
PRIMER_INTERNAL_1_SEQUENCE=CTCTGTCGCCCAGGTTGG
PRIMER_LEFT_1=83,24
PRIMER_RIGHT_1=157,21
PRIMER_INTERNAL_1=119,18
PRIMER_LEFT_1_TM=60.331
PRIMER_RIGHT_1_TM=58.902
PRIMER_INTERNAL_1_TM=62.868
PRIMER_LEFT_1_GC_PERCENT=29.167
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=6.434184
PRIMER_LEFT_2_PENALTY=4.147950
PRIMER_RIGHT_2_PENALTY=2.286234
PRIMER_INTERNAL_2_PENALTY=4.867800
PRIMER_LEFT_2_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG
PRIMER_RIGHT_2_SEQUENCE=TGAGATCACACCACTGCACT
PRIMER_INTERNAL_2_SEQUENCE=CTCTGTCGCCCAGGTTGG
PRIMER_LEFT_2=77,24
PRIMER_RIGHT_2=156,20
PRIMER_INTERNAL_2=119,18
PRIMER_LEFT_2_TM=59.852
PRIMER_RIGHT_2_TM=57.714
PRIMER_INTERNAL_2_TM=62.868
PRIMER_LEFT_2_GC_PERCENT=29.167
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=66.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=6.434184
PRIMER_LEFT_3_PENALTY=4.147950
PRIMER_RIGHT_3_PENALTY=2.286234
PRIMER_INTERNAL_3_PENALTY=4.867800
PRIMER_LEFT_3_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG
PRIMER_RIGHT_3_SEQUENCE=CTGAGATCACACCACTGCAC
PRIMER_INTERNAL_3_SEQUENCE=CTCTGTCGCCCAGGTTGG
PRIMER_LEFT_3=77,24
PRIMER_RIGHT_3=157,20
PRIMER_INTERNAL_3=119,18
PRIMER_LEFT_3_TM=59.852
PRIMER_RIGHT_3_TM=57.714
PRIMER_INTERNAL_3_TM=62.868
PRIMER_LEFT_3_GC_PERCENT=29.167
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=66.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=6.546818
PRIMER_LEFT_4_PENALTY=4.449085
PRIMER_RIGHT_4_PENALTY=2.097734
PRIMER_INTERNAL_4_PENALTY=4.867800
PRIMER_LEFT_4_SEQUENCE=TTGTCATTGTTGTTGTTGTTTTG
PRIMER_RIGHT_4_SEQUENCE=CTGAGATCACACCACTGCACT
PRIMER_INTERNAL_4_SEQUENCE=CTCTGTCGCCCAGGTTGG
PRIMER_LEFT_4=83,23
PRIMER_RIGHT_4=157,21
PRIMER_INTERNAL_4=119,18
PRIMER_LEFT_4_TM=58.551
PRIMER_RIGHT_4_TM=58.902
PRIMER_INTERNAL_4_TM=62.868
PRIMER_LEFT_4_GC_PERCENT=30.435
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH436
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGAAAACAAAAATTTCGTACAGTNCAAATAACATTTGGGAATTCCTTAAGTNACCCATAAAGTACAATATTCGATGTTTCGTGTNNACAACCCCAGGAAAAATGTTTAATGCCCGTAGTAATGTACCTGATAGGAAAAAAAGTGCATATGCCAAAATAGAATGTTTGATGTAAAGGAGAGACAGAGAGAGGAAAGAGAGAGAGAAAGAGAGAGAGACGAAAGAGAGAGAGGAAG
SEQUENCE_INCLUDED_REGION=34,236
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.982775
PRIMER_LEFT_0_PENALTY=0.699731
PRIMER_RIGHT_0_PENALTY=4.283044
PRIMER_INTERNAL_0_PENALTY=3.932182
PRIMER_LEFT_0_SEQUENCE=GGAAAAATGTTTAATGCCCG
PRIMER_RIGHT_0_SEQUENCE=CATCAAACATTCTATTTTGGCA
PRIMER_INTERNAL_0_SEQUENCE=CCTGATAGGAAAAAAAGTGCA
PRIMER_LEFT_0=131,20
PRIMER_RIGHT_0=205,22
PRIMER_INTERNAL_0=161,21
PRIMER_LEFT_0_TM=59.300
PRIMER_RIGHT_0_TM=57.717
PRIMER_INTERNAL_0_TM=57.068
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=31.818
PRIMER_INTERNAL_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=12.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=9.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=5.133665
PRIMER_LEFT_1_PENALTY=1.043085
PRIMER_RIGHT_1_PENALTY=4.090580
PRIMER_INTERNAL_1_PENALTY=4.222735
PRIMER_LEFT_1_SEQUENCE=GGAAAAAAAGTGCATATGCCA
PRIMER_RIGHT_1_SEQUENCE=TCCTCTCTCTCTTTCGTCTCTCTC
PRIMER_INTERNAL_1_SEQUENCE=GGAGAGACAGAGAGAGGAAAGAGA
PRIMER_LEFT_1=168,21
PRIMER_RIGHT_1=267,24
PRIMER_INTERNAL_1=210,24
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.909
PRIMER_INTERNAL_1_TM=59.777
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=5.144079
PRIMER_LEFT_2_PENALTY=0.699731
PRIMER_RIGHT_2_PENALTY=4.444347
PRIMER_INTERNAL_2_PENALTY=3.932182
PRIMER_LEFT_2_SEQUENCE=GGAAAAATGTTTAATGCCCG
PRIMER_RIGHT_2_SEQUENCE=ACATCAAACATTCTATTTTGGCA
PRIMER_INTERNAL_2_SEQUENCE=CCTGATAGGAAAAAAAGTGCA
PRIMER_LEFT_2=131,20
PRIMER_RIGHT_2=206,23
PRIMER_INTERNAL_2=161,21
PRIMER_LEFT_2_TM=59.300
PRIMER_RIGHT_2_TM=58.556
PRIMER_INTERNAL_2_TM=57.068
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_INTERNAL_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=9.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=5.327400
PRIMER_LEFT_3_PENALTY=4.627669
PRIMER_RIGHT_3_PENALTY=0.699731
PRIMER_INTERNAL_3_PENALTY=6.371570
PRIMER_LEFT_3_SEQUENCE=CAAATAACATTTGGGAATTCCTT
PRIMER_RIGHT_3_SEQUENCE=CGGGCATTAAACATTTTTCC
PRIMER_INTERNAL_3_SEQUENCE=AAAGTACAATATTCGATGTTTCGTG
PRIMER_LEFT_3=60,23
PRIMER_RIGHT_3=150,20
PRIMER_INTERNAL_3=94,25
PRIMER_LEFT_3_TM=58.372
PRIMER_RIGHT_3_TM=59.300
PRIMER_INTERNAL_3_TM=58.628
PRIMER_LEFT_3_GC_PERCENT=30.435
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=32.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=5.351938
PRIMER_LEFT_4_PENALTY=1.068894
PRIMER_RIGHT_4_PENALTY=4.283044
PRIMER_INTERNAL_4_PENALTY=3.932182
PRIMER_LEFT_4_SEQUENCE=GGAAAAATGTTTAATGCCCGT
PRIMER_RIGHT_4_SEQUENCE=CATCAAACATTCTATTTTGGCA
PRIMER_INTERNAL_4_SEQUENCE=CCTGATAGGAAAAAAAGTGCA
PRIMER_LEFT_4=131,21
PRIMER_RIGHT_4=205,22
PRIMER_INTERNAL_4=161,21
PRIMER_LEFT_4_TM=60.069
PRIMER_RIGHT_4_TM=57.717
PRIMER_INTERNAL_4_TM=57.068
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=31.818
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=9.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH437
SEQUENCE_TEMPLATE=TGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAGGACGGGCAAAGTTGAGTGGCTGAAAGTGTAGGGAACAGCATTTCATGCAGAGGGAATTGCATGTCCAAAGCCTGGCATGCAGCAAGC
SEQUENCE_INCLUDED_REGION=37,93
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.127660
PRIMER_LEFT_0_PENALTY=1.046699
PRIMER_RIGHT_0_PENALTY=0.080962
PRIMER_INTERNAL_0_PENALTY=0.194598
PRIMER_LEFT_0_SEQUENCE=AAGGACGGGCAAAGTTGAGT
PRIMER_RIGHT_0_SEQUENCE=AGGCTTTGGACATGCAATTC
PRIMER_INTERNAL_0_SEQUENCE=GGAACAGCATTTCATGCAGA
PRIMER_LEFT_0=40,20
PRIMER_RIGHT_0=115,20
PRIMER_INTERNAL_0=74,20
PRIMER_LEFT_0_TM=61.047
PRIMER_RIGHT_0_TM=60.081
PRIMER_INTERNAL_0_TM=59.805
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=1.306828
PRIMER_LEFT_1_PENALTY=1.239392
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_INTERNAL_1_PENALTY=1.276629
PRIMER_LEFT_1_SEQUENCE=CTGAAGGACGGGCAAAGTT
PRIMER_RIGHT_1_SEQUENCE=CTTTGGACATGCAATTCCCT
PRIMER_INTERNAL_1_SEQUENCE=TAGGGAACAGCATTTCATGC
PRIMER_LEFT_1=37,19
PRIMER_RIGHT_1=112,20
PRIMER_INTERNAL_1=71,20
PRIMER_LEFT_1_TM=60.239
PRIMER_RIGHT_1_TM=59.933
PRIMER_INTERNAL_1_TM=58.723
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=1.320353
PRIMER_LEFT_2_PENALTY=1.239392
PRIMER_RIGHT_2_PENALTY=0.080962
PRIMER_INTERNAL_2_PENALTY=0.194598
PRIMER_LEFT_2_SEQUENCE=AAGGACGGGCAAAGTTGAG
PRIMER_RIGHT_2_SEQUENCE=AGGCTTTGGACATGCAATTC
PRIMER_INTERNAL_2_SEQUENCE=GGAACAGCATTTCATGCAGA
PRIMER_LEFT_2=40,19
PRIMER_RIGHT_2=115,20
PRIMER_INTERNAL_2=74,20
PRIMER_LEFT_2_TM=60.239
PRIMER_RIGHT_2_TM=60.081
PRIMER_INTERNAL_2_TM=59.805
PRIMER_LEFT_2_GC_PERCENT=52.632
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=1.320353
PRIMER_LEFT_3_PENALTY=1.239392
PRIMER_RIGHT_3_PENALTY=0.080962
PRIMER_INTERNAL_3_PENALTY=0.194598
PRIMER_LEFT_3_SEQUENCE=CTGAAGGACGGGCAAAGTT
PRIMER_RIGHT_3_SEQUENCE=AGGCTTTGGACATGCAATTC
PRIMER_INTERNAL_3_SEQUENCE=GGAACAGCATTTCATGCAGA
PRIMER_LEFT_3=37,19
PRIMER_RIGHT_3=115,20
PRIMER_INTERNAL_3=74,20
PRIMER_LEFT_3_TM=60.239
PRIMER_RIGHT_3_TM=60.081
PRIMER_INTERNAL_3_TM=59.805
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=1.368717
PRIMER_LEFT_4_PENALTY=1.287755
PRIMER_RIGHT_4_PENALTY=0.080962
PRIMER_INTERNAL_4_PENALTY=0.194598
PRIMER_LEFT_4_SEQUENCE=AGGACGGGCAAAGTTGAGT
PRIMER_RIGHT_4_SEQUENCE=AGGCTTTGGACATGCAATTC
PRIMER_INTERNAL_4_SEQUENCE=GGAACAGCATTTCATGCAGA
PRIMER_LEFT_4=41,19
PRIMER_RIGHT_4=115,20
PRIMER_INTERNAL_4=74,20
PRIMER_LEFT_4_TM=59.712
PRIMER_RIGHT_4_TM=60.081
PRIMER_INTERNAL_4_TM=59.805
PRIMER_LEFT_4_GC_PERCENT=52.632
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH441
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATACTAGGAACTCAGTTCCTTTTTCTTAAAACAACATAAGACAGGAAAACATCACATCCTTTAGGTNNCGAAGACTGAGCCCATTTTAAACGCCGTTATTTTNCCCANCCCACCGNCCCAAGCCCCCCGCCCCCCGCCCCGAGCGAATGCTGTTATAACTGGTTTATCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG
SEQUENCE_INCLUDED_REGION=36,134
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.224529
PRIMER_LEFT_0_PENALTY=6.453153
PRIMER_RIGHT_0_PENALTY=0.771376
PRIMER_INTERNAL_0_PENALTY=3.615875
PRIMER_LEFT_0_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAAA
PRIMER_RIGHT_0_SEQUENCE=GCGTTTAAAATGGGCTCAGT
PRIMER_INTERNAL_0_SEQUENCE=GACAGGAAAACATCACATCCTTT
PRIMER_LEFT_0=44,25
PRIMER_RIGHT_0=130,20
PRIMER_INTERNAL_0=77,23
PRIMER_LEFT_0_TM=58.547
PRIMER_RIGHT_0_TM=59.229
PRIMER_INTERNAL_0_TM=59.384
PRIMER_LEFT_0_GC_PERCENT=32.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=11.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=7.246225
PRIMER_LEFT_1_PENALTY=6.474849
PRIMER_RIGHT_1_PENALTY=0.771376
PRIMER_INTERNAL_1_PENALTY=3.615875
PRIMER_LEFT_1_SEQUENCE=TCAGTTCCTTTTTCTTAAAACAACA
PRIMER_RIGHT_1_SEQUENCE=GCGTTTAAAATGGGCTCAGT
PRIMER_INTERNAL_1_SEQUENCE=GACAGGAAAACATCACATCCTTT
PRIMER_LEFT_1=49,25
PRIMER_RIGHT_1=130,20
PRIMER_INTERNAL_1=77,23
PRIMER_LEFT_1_TM=58.525
PRIMER_RIGHT_1_TM=59.229
PRIMER_INTERNAL_1_TM=59.384
PRIMER_LEFT_1_GC_PERCENT=28.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=7.423825
PRIMER_LEFT_2_PENALTY=6.652448
PRIMER_RIGHT_2_PENALTY=0.771376
PRIMER_INTERNAL_2_PENALTY=3.615875
PRIMER_LEFT_2_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAA
PRIMER_RIGHT_2_SEQUENCE=GCGTTTAAAATGGGCTCAGT
PRIMER_INTERNAL_2_SEQUENCE=GACAGGAAAACATCACATCCTTT
PRIMER_LEFT_2=44,24
PRIMER_RIGHT_2=130,20
PRIMER_INTERNAL_2=77,23
PRIMER_LEFT_2_TM=57.348
PRIMER_RIGHT_2_TM=59.229
PRIMER_INTERNAL_2_TM=59.384
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=11.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=7.535615
PRIMER_LEFT_3_PENALTY=6.453153
PRIMER_RIGHT_3_PENALTY=1.082462
PRIMER_INTERNAL_3_PENALTY=3.615875
PRIMER_LEFT_3_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAAA
PRIMER_RIGHT_3_SEQUENCE=AATAACGGCGTTTAAAATGGG
PRIMER_INTERNAL_3_SEQUENCE=GACAGGAAAACATCACATCCTTT
PRIMER_LEFT_3=44,25
PRIMER_RIGHT_3=137,21
PRIMER_INTERNAL_3=77,23
PRIMER_LEFT_3_TM=58.547
PRIMER_RIGHT_3_TM=60.082
PRIMER_INTERNAL_3_TM=59.384
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=11.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=7.557311
PRIMER_LEFT_4_PENALTY=6.474849
PRIMER_RIGHT_4_PENALTY=1.082462
PRIMER_INTERNAL_4_PENALTY=3.615875
PRIMER_LEFT_4_SEQUENCE=TCAGTTCCTTTTTCTTAAAACAACA
PRIMER_RIGHT_4_SEQUENCE=AATAACGGCGTTTAAAATGGG
PRIMER_INTERNAL_4_SEQUENCE=GACAGGAAAACATCACATCCTTT
PRIMER_LEFT_4=49,25
PRIMER_RIGHT_4=137,21
PRIMER_INTERNAL_4=77,23
PRIMER_LEFT_4_TM=58.525
PRIMER_RIGHT_4_TM=60.082
PRIMER_INTERNAL_4_TM=59.384
PRIMER_LEFT_4_GC_PERCENT=28.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH459
SEQUENCE_TEMPLATE=AGACAACCCAGACAGTGAGACACTAATCAGGAGTCTAATACAATACAAAAGAGCAATGAGCATATGAACCATCATATGCTGTTATCATTGAGCCACTTATACCCTGCATGATCAAGCACCCAGTGCCCTTGTAGTCTACATCCCAGTACGAATAAAAGCCAGAGTATTGACTCTCTTTAAAAGGTAAATTTTAATTTTTTCCTTACCACTAAGTATAATTCAGATAGCCCAGG
SEQUENCE_INCLUDED_REGION=0,233
P3_COMMENT=CL is CAGACAGTGAGACACTAATCAGG
P3_COMMENT=CR is AGTCAATACTCTGGCTTTTATTCG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.829603
PRIMER_LEFT_0_PENALTY=2.262496
PRIMER_RIGHT_0_PENALTY=0.567107
PRIMER_INTERNAL_0_PENALTY=0.703016
PRIMER_LEFT_0_SEQUENCE=GAGCAATGAGCATATGAACCA
PRIMER_RIGHT_0_SEQUENCE=GACTACAAGGGCACTGGGTG
PRIMER_INTERNAL_0_SEQUENCE=TGAGCCACTTATACCCTGCA
PRIMER_LEFT_0=50,21
PRIMER_RIGHT_0=135,20
PRIMER_INTERNAL_0=88,20
PRIMER_LEFT_0_TM=58.738
PRIMER_RIGHT_0_TM=60.567
PRIMER_INTERNAL_0_TM=59.297
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=2.831250
PRIMER_LEFT_1_PENALTY=2.264143
PRIMER_RIGHT_1_PENALTY=0.567107
PRIMER_INTERNAL_1_PENALTY=0.703016
PRIMER_LEFT_1_SEQUENCE=AGAGCAATGAGCATATGAACCA
PRIMER_RIGHT_1_SEQUENCE=GACTACAAGGGCACTGGGTG
PRIMER_INTERNAL_1_SEQUENCE=TGAGCCACTTATACCCTGCA
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=135,20
PRIMER_INTERNAL_1=88,20
PRIMER_LEFT_1_TM=59.736
PRIMER_RIGHT_1_TM=60.567
PRIMER_INTERNAL_1_TM=59.297
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=3.184342
PRIMER_LEFT_2_PENALTY=0.703016
PRIMER_RIGHT_2_PENALTY=2.481326
PRIMER_INTERNAL_2_PENALTY=0.567107
PRIMER_LEFT_2_SEQUENCE=TGAGCCACTTATACCCTGCA
PRIMER_RIGHT_2_SEQUENCE=TCTGGCTTTTATTCGTACTGGG
PRIMER_INTERNAL_2_SEQUENCE=CACCCAGTGCCCTTGTAGTC
PRIMER_LEFT_2=88,20
PRIMER_RIGHT_2=162,22
PRIMER_INTERNAL_2=116,20
PRIMER_LEFT_2_TM=59.297
PRIMER_RIGHT_2_TM=60.481
PRIMER_INTERNAL_2_TM=60.567
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=3.433889
PRIMER_LEFT_3_PENALTY=2.866782
PRIMER_RIGHT_3_PENALTY=0.567107
PRIMER_INTERNAL_3_PENALTY=0.703016
PRIMER_LEFT_3_SEQUENCE=GCAATGAGCATATGAACCATCA
PRIMER_RIGHT_3_SEQUENCE=GACTACAAGGGCACTGGGTG
PRIMER_INTERNAL_3_SEQUENCE=TGAGCCACTTATACCCTGCA
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=135,20
PRIMER_INTERNAL_3=88,20
PRIMER_LEFT_3_TM=60.867
PRIMER_RIGHT_3_TM=60.567
PRIMER_INTERNAL_3_TM=59.297
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=3.493466
PRIMER_LEFT_4_PENALTY=2.926360
PRIMER_RIGHT_4_PENALTY=0.567107
PRIMER_INTERNAL_4_PENALTY=0.703016
PRIMER_LEFT_4_SEQUENCE=AGCAATGAGCATATGAACCATC
PRIMER_RIGHT_4_SEQUENCE=GACTACAAGGGCACTGGGTG
PRIMER_INTERNAL_4_SEQUENCE=TGAGCCACTTATACCCTGCA
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=135,20
PRIMER_INTERNAL_4=88,20
PRIMER_LEFT_4_TM=59.074
PRIMER_RIGHT_4_TM=60.567
PRIMER_INTERNAL_4_TM=59.297
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH465
SEQUENCE_TEMPLATE=CGNNNTTGATGCTGCAGGTCGACTCTAGAGGATCCCCCTATGTGCAAATCAGACCACAAGAGAGGANGGAAATTGAATCAATGGAATGAGAATTATTTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCC
SEQUENCE_INCLUDED_REGION=37,64
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH471
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGCTCTGATTTTACCTCACCTCTAGAGTNNGTTTTATATGTAGACTACTTAAGGCACTTCCTTCGGACAGTGTTGGGAGAGACAGTGATAGATTTTTTTTCAGATGCCTGACTTGCTTTTTTTCAGATGTCATTACANTGTACTGAAATACTTTTTTCAAATACGTAATAATTCAGATAGATAACCATGTGTGTTCAGGGGACCCCCAGATGATCCTGAGGTTCAGTGACTCACCAGAAGTACCCACGGAACTCAGCATATAGAAACATGTTTTAGTTTGCTGAACTTTTATCCTGTTATTTTACTGAAG
SEQUENCE_INCLUDED_REGION=36,313
P3_COMMENT=CL is GTGCTCTGATTTTACCTCACC
P3_COMMENT=CR is TCTATATGCTGAGTTCCGTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.704234
PRIMER_LEFT_0_PENALTY=0.700912
PRIMER_RIGHT_0_PENALTY=0.003322
PRIMER_INTERNAL_0_PENALTY=0.539612
PRIMER_LEFT_0_SEQUENCE=ACCATGTGTGTTCAGGGGAC
PRIMER_RIGHT_0_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
PRIMER_INTERNAL_0_SEQUENCE=CCCAGATGATCCTGAGGTTC
PRIMER_LEFT_0=222,20
PRIMER_RIGHT_0=297,20
PRIMER_INTERNAL_0=243,20
PRIMER_LEFT_0_TM=60.701
PRIMER_RIGHT_0_TM=59.997
PRIMER_INTERNAL_0_TM=59.460
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=0.740939
PRIMER_LEFT_1_PENALTY=0.737617
PRIMER_RIGHT_1_PENALTY=0.003322
PRIMER_INTERNAL_1_PENALTY=0.539612
PRIMER_LEFT_1_SEQUENCE=TAACCATGTGTGTTCAGGGG
PRIMER_RIGHT_1_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
PRIMER_INTERNAL_1_SEQUENCE=CCCAGATGATCCTGAGGTTC
PRIMER_LEFT_1=220,20
PRIMER_RIGHT_1=297,20
PRIMER_INTERNAL_1=243,20
PRIMER_LEFT_1_TM=59.262
PRIMER_RIGHT_1_TM=59.997
PRIMER_INTERNAL_1_TM=59.460
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=1.218783
PRIMER_LEFT_2_PENALTY=1.215461
PRIMER_RIGHT_2_PENALTY=0.003322
PRIMER_INTERNAL_2_PENALTY=0.539612
PRIMER_LEFT_2_SEQUENCE=CCATGTGTGTTCAGGGGAC
PRIMER_RIGHT_2_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
PRIMER_INTERNAL_2_SEQUENCE=CCCAGATGATCCTGAGGTTC
PRIMER_LEFT_2=223,19
PRIMER_RIGHT_2=297,20
PRIMER_INTERNAL_2=243,20
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=59.997
PRIMER_INTERNAL_2_TM=59.460
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=7.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=1.218783
PRIMER_LEFT_3_PENALTY=1.215461
PRIMER_RIGHT_3_PENALTY=0.003322
PRIMER_INTERNAL_3_PENALTY=0.539612
PRIMER_LEFT_3_SEQUENCE=ACCATGTGTGTTCAGGGGA
PRIMER_RIGHT_3_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
PRIMER_INTERNAL_3_SEQUENCE=CCCAGATGATCCTGAGGTTC
PRIMER_LEFT_3=222,19
PRIMER_RIGHT_3=297,20
PRIMER_INTERNAL_3=243,20
PRIMER_LEFT_3_TM=59.785
PRIMER_RIGHT_3_TM=59.997
PRIMER_INTERNAL_3_TM=59.460
PRIMER_LEFT_3_GC_PERCENT=52.632
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=1.241404
PRIMER_LEFT_4_PENALTY=1.238082
PRIMER_RIGHT_4_PENALTY=0.003322
PRIMER_INTERNAL_4_PENALTY=0.539612
PRIMER_LEFT_4_SEQUENCE=AACCATGTGTGTTCAGGGGA
PRIMER_RIGHT_4_SEQUENCE=ATGCTGAGTTCCGTGGGTAC
PRIMER_INTERNAL_4_SEQUENCE=CCCAGATGATCCTGAGGTTC
PRIMER_LEFT_4=221,20
PRIMER_RIGHT_4=297,20
PRIMER_INTERNAL_4=243,20
PRIMER_LEFT_4_TM=61.238
PRIMER_RIGHT_4_TM=59.997
PRIMER_INTERNAL_4_TM=59.460
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH476
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGCCCTTCCCGGCATCAACCACCAGGCCCGAGTGTGGGGCCCGAGTTGCTGGCTGCCCTGAGTGCCCTGGTCCCTGGGGCCCCCATGGGAGATCAAGGGGCTCAGGAGAGGGCATGGGACTCAGGAGGTCCCGGGGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
SEQUENCE_INCLUDED_REGION=36,141
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.554176
PRIMER_LEFT_0_PENALTY=3.262057
PRIMER_RIGHT_0_PENALTY=2.292120
PRIMER_INTERNAL_0_PENALTY=1.271648
PRIMER_LEFT_0_SEQUENCE=CTGAGTGCCCTGGTCCCT
PRIMER_RIGHT_0_SEQUENCE=GGGACCTCCTGAGTCCCAT
PRIMER_INTERNAL_0_SEQUENCE=GATCAAGGGGCTCAGGAGAG
PRIMER_LEFT_0=96,18
PRIMER_RIGHT_0=170,19
PRIMER_INTERNAL_0=129,20
PRIMER_LEFT_0_TM=61.262
PRIMER_RIGHT_0_TM=61.292
PRIMER_INTERNAL_0_TM=61.272
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=6.317680
PRIMER_LEFT_1_PENALTY=3.262057
PRIMER_RIGHT_1_PENALTY=3.055624
PRIMER_INTERNAL_1_PENALTY=1.271648
PRIMER_LEFT_1_SEQUENCE=CTGAGTGCCCTGGTCCCT
PRIMER_RIGHT_1_SEQUENCE=GGGACCTCCTGAGTCCCA
PRIMER_INTERNAL_1_SEQUENCE=GATCAAGGGGCTCAGGAGAG
PRIMER_LEFT_1=96,18
PRIMER_RIGHT_1=170,18
PRIMER_INTERNAL_1=129,20
PRIMER_LEFT_1_TM=61.262
PRIMER_RIGHT_1_TM=61.056
PRIMER_INTERNAL_1_TM=61.272
PRIMER_LEFT_1_GC_PERCENT=66.667
PRIMER_RIGHT_1_GC_PERCENT=66.667
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=6.847421
PRIMER_LEFT_2_PENALTY=4.486184
PRIMER_RIGHT_2_PENALTY=2.361236
PRIMER_INTERNAL_2_PENALTY=3.262057
PRIMER_LEFT_2_SEQUENCE=CTTCCCGGCATCAACCAC
PRIMER_RIGHT_2_SEQUENCE=GAGCCCCTTGATCTCCCAT
PRIMER_INTERNAL_2_SEQUENCE=CTGAGTGCCCTGGTCCCT
PRIMER_LEFT_2=43,18
PRIMER_RIGHT_2=141,19
PRIMER_INTERNAL_2=96,18
PRIMER_LEFT_2_TM=62.486
PRIMER_RIGHT_2_TM=61.361
PRIMER_INTERNAL_2_TM=61.262
PRIMER_LEFT_2_GC_PERCENT=61.111
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_INTERNAL_2_GC_PERCENT=66.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=7.071457
PRIMER_LEFT_3_PENALTY=4.486184
PRIMER_RIGHT_3_PENALTY=2.585272
PRIMER_INTERNAL_3_PENALTY=3.262057
PRIMER_LEFT_3_SEQUENCE=CTTCCCGGCATCAACCAC
PRIMER_RIGHT_3_SEQUENCE=AGCCCCTTGATCTCCCAT
PRIMER_INTERNAL_3_SEQUENCE=CTGAGTGCCCTGGTCCCT
PRIMER_LEFT_3=43,18
PRIMER_RIGHT_3=140,18
PRIMER_INTERNAL_3=96,18
PRIMER_LEFT_3_TM=62.486
PRIMER_RIGHT_3_TM=59.415
PRIMER_INTERNAL_3_TM=61.262
PRIMER_LEFT_3_GC_PERCENT=61.111
PRIMER_RIGHT_3_GC_PERCENT=55.556
PRIMER_INTERNAL_3_GC_PERCENT=66.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=7.623076
PRIMER_LEFT_4_PENALTY=4.486184
PRIMER_RIGHT_4_PENALTY=3.136891
PRIMER_INTERNAL_4_PENALTY=3.262057
PRIMER_LEFT_4_SEQUENCE=CTTCCCGGCATCAACCAC
PRIMER_RIGHT_4_SEQUENCE=GAGCCCCTTGATCTCCCA
PRIMER_INTERNAL_4_SEQUENCE=CTGAGTGCCCTGGTCCCT
PRIMER_LEFT_4=43,18
PRIMER_RIGHT_4=141,18
PRIMER_INTERNAL_4=96,18
PRIMER_LEFT_4_TM=62.486
PRIMER_RIGHT_4_TM=61.137
PRIMER_INTERNAL_4_TM=61.262
PRIMER_LEFT_4_GC_PERCENT=61.111
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH479
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGATTTCAGAATCACTATAGACAAGTGACCGCTATTGTTTCCTCTTCTCCCCTTTATAAATGGGAGAATTTACTATCCTACGCCTATTTCACCATTGTATATTGGTTCTACATGAGTAGATCACGTGTCTCTTTAGTTCACAAGTCTTTAAGAGGAAATGCACTTGAATATGGGCTGGACTTTGTGATTTGCTCCATCATGAAAATGTAGAGGAAGTAATATTCTAGGACTTCCAAGGCTAACTTATGAAAAGCCTTGCAGTTTTTGTCTGGATGTCTGCATCTCTTGGCATGTTTCTTCCTTAGCAACTCCC
SEQUENCE_INCLUDED_REGION=36,315
P3_COMMENT=CL is TTGTTTCCTCTTCTCCCCTT
P3_COMMENT=CR is GCTAAGGAAGAAACATGCCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.452342
PRIMER_LEFT_0_PENALTY=0.645726
PRIMER_RIGHT_0_PENALTY=0.806616
PRIMER_INTERNAL_0_PENALTY=4.026278
PRIMER_LEFT_0_SEQUENCE=GGCTGGACTTTGTGATTTGC
PRIMER_RIGHT_0_SEQUENCE=CCAGACAAAAACTGCAAGGC
PRIMER_INTERNAL_0_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA
PRIMER_LEFT_0=210,20
PRIMER_RIGHT_0=309,20
PRIMER_INTERNAL_0=264,24
PRIMER_LEFT_0_TM=60.646
PRIMER_RIGHT_0_TM=60.807
PRIMER_INTERNAL_0_TM=60.026
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=1.871422
PRIMER_LEFT_1_PENALTY=1.064806
PRIMER_RIGHT_1_PENALTY=0.806616
PRIMER_INTERNAL_1_PENALTY=4.026278
PRIMER_LEFT_1_SEQUENCE=TGTGATTTGCTCCATCATGAA
PRIMER_RIGHT_1_SEQUENCE=CCAGACAAAAACTGCAAGGC
PRIMER_INTERNAL_1_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA
PRIMER_LEFT_1=220,21
PRIMER_RIGHT_1=309,20
PRIMER_INTERNAL_1=264,24
PRIMER_LEFT_1_TM=60.065
PRIMER_RIGHT_1_TM=60.807
PRIMER_INTERNAL_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=1.871422
PRIMER_LEFT_2_PENALTY=1.064806
PRIMER_RIGHT_2_PENALTY=0.806616
PRIMER_INTERNAL_2_PENALTY=4.026278
PRIMER_LEFT_2_SEQUENCE=TTGTGATTTGCTCCATCATGA
PRIMER_RIGHT_2_SEQUENCE=CCAGACAAAAACTGCAAGGC
PRIMER_INTERNAL_2_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA
PRIMER_LEFT_2=219,21
PRIMER_RIGHT_2=309,20
PRIMER_INTERNAL_2=264,24
PRIMER_LEFT_2_TM=60.065
PRIMER_RIGHT_2_TM=60.807
PRIMER_INTERNAL_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_3_PENALTY=1.889809
PRIMER_LEFT_3_PENALTY=0.495173
PRIMER_RIGHT_3_PENALTY=1.394636
PRIMER_INTERNAL_3_PENALTY=4.431064
PRIMER_LEFT_3_SEQUENCE=GGGCTGGACTTTGTGATTTG
PRIMER_RIGHT_3_SEQUENCE=CAAAAACTGCAAGGCTTTTCA
PRIMER_INTERNAL_3_SEQUENCE=TCCATCATGAAAATGTAGAGGAA
PRIMER_LEFT_3=209,20
PRIMER_RIGHT_3=304,21
PRIMER_INTERNAL_3=230,23
PRIMER_LEFT_3_TM=60.495
PRIMER_RIGHT_3_TM=60.395
PRIMER_INTERNAL_3_TM=58.569
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_INTERNAL_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=1.889809
PRIMER_LEFT_4_PENALTY=0.495173
PRIMER_RIGHT_4_PENALTY=1.394636
PRIMER_INTERNAL_4_PENALTY=3.020535
PRIMER_LEFT_4_SEQUENCE=TGGGCTGGACTTTGTGATTT
PRIMER_RIGHT_4_SEQUENCE=CAAAAACTGCAAGGCTTTTCA
PRIMER_INTERNAL_4_SEQUENCE=GCTCCATCATGAAAATGTAGAGG
PRIMER_LEFT_4=208,20
PRIMER_RIGHT_4=304,21
PRIMER_INTERNAL_4=228,23
PRIMER_LEFT_4_TM=60.495
PRIMER_RIGHT_4_TM=60.395
PRIMER_INTERNAL_4_TM=59.979
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=97
=
SEQUENCE_ID=MH480
SEQUENCE_TEMPLATE=CAGTAACTTTTCATAAAGCAACCTCACCTCTACCATTATGGTGCTCACTGTCTGAATTATCCTCCTCTTCTAGAAACTATATTTACAGTTTGTCGATGATCCTGGAAATGACTACTCTGTTAAATCTGTGAAACTGATCTTGAGGTGTTTTATTTATTTATTTTTTCTATCAAGACTAAAATTTCCCAGAGGGGTACCGAG
SEQUENCE_INCLUDED_REGION=0,126
P3_COMMENT=CL is AAGCAACCTCACCTCTACCA
P3_COMMENT=CR is GTAGTCATTTCCAGGATCATCG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.698290
PRIMER_LEFT_0_PENALTY=2.149011
PRIMER_RIGHT_0_PENALTY=0.549279
PRIMER_INTERNAL_0_PENALTY=3.018344
PRIMER_LEFT_0_SEQUENCE=AAAGCAACCTCACCTCTACCA
PRIMER_RIGHT_0_SEQUENCE=CATTTCCAGGATCATCGACA
PRIMER_INTERNAL_0_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
PRIMER_LEFT_0=14,21
PRIMER_RIGHT_0=109,20
PRIMER_INTERNAL_0=39,23
PRIMER_LEFT_0_TM=58.851
PRIMER_RIGHT_0_TM=59.451
PRIMER_INTERNAL_0_TM=60.018
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=3.063067
PRIMER_LEFT_1_PENALTY=2.149011
PRIMER_RIGHT_1_PENALTY=0.914056
PRIMER_INTERNAL_1_PENALTY=3.018344
PRIMER_LEFT_1_SEQUENCE=AAAGCAACCTCACCTCTACCA
PRIMER_RIGHT_1_SEQUENCE=CCAGGATCATCGACAAACTG
PRIMER_INTERNAL_1_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
PRIMER_LEFT_1=14,21
PRIMER_RIGHT_1=104,20
PRIMER_INTERNAL_1=39,23
PRIMER_LEFT_1_TM=58.851
PRIMER_RIGHT_1_TM=59.086
PRIMER_INTERNAL_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=3.099713
PRIMER_LEFT_2_PENALTY=2.149011
PRIMER_RIGHT_2_PENALTY=0.950702
PRIMER_INTERNAL_2_PENALTY=3.018344
PRIMER_LEFT_2_SEQUENCE=AAAGCAACCTCACCTCTACCA
PRIMER_RIGHT_2_SEQUENCE=TTTCCAGGATCATCGACAAA
PRIMER_INTERNAL_2_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
PRIMER_LEFT_2=14,21
PRIMER_RIGHT_2=107,20
PRIMER_INTERNAL_2=39,23
PRIMER_LEFT_2_TM=58.851
PRIMER_RIGHT_2_TM=59.049
PRIMER_INTERNAL_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=3.162285
PRIMER_LEFT_3_PENALTY=2.613006
PRIMER_RIGHT_3_PENALTY=0.549279
PRIMER_INTERNAL_3_PENALTY=3.018344
PRIMER_LEFT_3_SEQUENCE=AAGCAACCTCACCTCTACCA
PRIMER_RIGHT_3_SEQUENCE=CATTTCCAGGATCATCGACA
PRIMER_INTERNAL_3_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
PRIMER_LEFT_3=15,20
PRIMER_RIGHT_3=109,20
PRIMER_INTERNAL_3=39,23
PRIMER_LEFT_3_TM=57.387
PRIMER_RIGHT_3_TM=59.451
PRIMER_INTERNAL_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=3.167523
PRIMER_LEFT_4_PENALTY=2.149011
PRIMER_RIGHT_4_PENALTY=1.018512
PRIMER_INTERNAL_4_PENALTY=3.018344
PRIMER_LEFT_4_SEQUENCE=AAAGCAACCTCACCTCTACCA
PRIMER_RIGHT_4_SEQUENCE=CCAGGATCATCGACAAACTGT
PRIMER_INTERNAL_4_SEQUENCE=GGTGCTCACTGTCTGAATTATCC
PRIMER_LEFT_4=14,21
PRIMER_RIGHT_4=104,21
PRIMER_INTERNAL_4=39,23
PRIMER_LEFT_4_TM=58.851
PRIMER_RIGHT_4_TM=59.981
PRIMER_INTERNAL_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH481
SEQUENCE_TEMPLATE=AATGAAAGAAGATCTGAGAGACATGTGATCTTATTATGTTCGTTGTAAATACACAGAATACTGGAAACAACATAAAGCCAAATAAAAAATGATCATTTTTATAATGTATGGAATAACTGTGCAATTTTTCTAGTACAATAAAATAATTTAAATCTATATTGTATGATTGCATGGCAGTTCACGATATATTAATAAAAA
SEQUENCE_INCLUDED_REGION=0,198
P3_COMMENT=CL is GAGACATGTGATCTTATTATGTTCG
P3_COMMENT=CR is ATATATCGTGAACTGCCATGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=11.414870
PRIMER_LEFT_0_PENALTY=5.064245
PRIMER_RIGHT_0_PENALTY=6.350625
PRIMER_INTERNAL_0_PENALTY=6.911248
PRIMER_LEFT_0_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
PRIMER_RIGHT_0_SEQUENCE=TCATTTTTTATTTGGCTTTATGTTG
PRIMER_INTERNAL_0_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
PRIMER_LEFT_0=2,25
PRIMER_RIGHT_0=91,25
PRIMER_INTERNAL_0=39,26
PRIMER_LEFT_0_TM=59.936
PRIMER_RIGHT_0_TM=58.649
PRIMER_INTERNAL_0_TM=59.089
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=24.000
PRIMER_INTERNAL_0_GC_PERCENT=34.615
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=11.454780
PRIMER_LEFT_1_PENALTY=5.064245
PRIMER_RIGHT_1_PENALTY=6.390536
PRIMER_INTERNAL_1_PENALTY=6.911248
PRIMER_LEFT_1_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
PRIMER_RIGHT_1_SEQUENCE=TGATCATTTTTTATTTGGCTTTATG
PRIMER_INTERNAL_1_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
PRIMER_LEFT_1=2,25
PRIMER_RIGHT_1=94,25
PRIMER_INTERNAL_1=39,26
PRIMER_LEFT_1_TM=59.936
PRIMER_RIGHT_1_TM=58.609
PRIMER_INTERNAL_1_TM=59.089
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=24.000
PRIMER_INTERNAL_1_GC_PERCENT=34.615
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=11.509976
PRIMER_LEFT_2_PENALTY=5.064245
PRIMER_RIGHT_2_PENALTY=6.445731
PRIMER_INTERNAL_2_PENALTY=6.911248
PRIMER_LEFT_2_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
PRIMER_RIGHT_2_SEQUENCE=AAATGATCATTTTTTATTTGGCTTT
PRIMER_INTERNAL_2_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
PRIMER_LEFT_2=2,25
PRIMER_RIGHT_2=97,25
PRIMER_INTERNAL_2=39,26
PRIMER_LEFT_2_TM=59.936
PRIMER_RIGHT_2_TM=58.554
PRIMER_INTERNAL_2_TM=59.089
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=20.000
PRIMER_INTERNAL_2_GC_PERCENT=34.615
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=12.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=11.661126
PRIMER_LEFT_3_PENALTY=5.064245
PRIMER_RIGHT_3_PENALTY=6.596881
PRIMER_INTERNAL_3_PENALTY=6.911248
PRIMER_LEFT_3_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
PRIMER_RIGHT_3_SEQUENCE=AATGATCATTTTTTATTTGGCTTT
PRIMER_INTERNAL_3_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
PRIMER_LEFT_3=2,25
PRIMER_RIGHT_3=96,24
PRIMER_INTERNAL_3=39,26
PRIMER_LEFT_3_TM=59.936
PRIMER_RIGHT_3_TM=57.403
PRIMER_INTERNAL_3_TM=59.089
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=20.833
PRIMER_INTERNAL_3_GC_PERCENT=34.615
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=10.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=11.661126
PRIMER_LEFT_4_PENALTY=5.064245
PRIMER_RIGHT_4_PENALTY=6.596881
PRIMER_INTERNAL_4_PENALTY=6.911248
PRIMER_LEFT_4_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG
PRIMER_RIGHT_4_SEQUENCE=AAATGATCATTTTTTATTTGGCTT
PRIMER_INTERNAL_4_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA
PRIMER_LEFT_4=2,25
PRIMER_RIGHT_4=97,24
PRIMER_INTERNAL_4=39,26
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=57.403
PRIMER_INTERNAL_4_TM=59.089
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=20.833
PRIMER_INTERNAL_4_GC_PERCENT=34.615
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=12.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH484
SEQUENCE_TEMPLATE=TCCATTAAAGAGACATAAGATCACATCACTTCTGTGCTCAAAACCCCACAATGACTCTCTATTTCCCAGAGGGAAAGCCAGTCTTTACCAGAGTCTACAAAGCCTACACACTCGATCCCTGTTGTTTCTTGACCTCATTTCTTACATCTTCTCCAGGNCCACTCCACTCCAGCCACACAGCCTCCTTGCTTTTCCTTGAAAACACCAGGCACTCACACACTGCGAGGCTTTGAAGTGGCTGTTCTCTCTGCCTGGAATATTTCAATACATGTATCTGCATAGGGGTACCGAGCTCGAATTCGTAATCATGG
SEQUENCE_INCLUDED_REGION=0,162
P3_COMMENT=CL is CCATTAAAGAGACATAAGATCACA
P3_COMMENT=CR is TGGAGAAGATGTAAGAAATGAGG
P3_COMMENT=CM is CACTTCTGTGCTCAAAACCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.039952
PRIMER_LEFT_0_PENALTY=0.361767
PRIMER_RIGHT_0_PENALTY=0.678186
PRIMER_INTERNAL_0_PENALTY=0.390581
PRIMER_LEFT_0_SEQUENCE=GCTCAAAACCCCACAATGAC
PRIMER_RIGHT_0_SEQUENCE=CAGGGATCGAGTGTGTAGGC
PRIMER_INTERNAL_0_SEQUENCE=TATTTCCCAGAGGGAAAGCC
PRIMER_LEFT_0=35,20
PRIMER_RIGHT_0=120,20
PRIMER_INTERNAL_0=59,20
PRIMER_LEFT_0_TM=60.362
PRIMER_RIGHT_0_TM=60.678
PRIMER_INTERNAL_0_TM=60.391
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=9.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.3000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=1.195846
PRIMER_LEFT_1_PENALTY=0.361767
PRIMER_RIGHT_1_PENALTY=0.834079
PRIMER_INTERNAL_1_PENALTY=0.390581
PRIMER_LEFT_1_SEQUENCE=GCTCAAAACCCCACAATGAC
PRIMER_RIGHT_1_SEQUENCE=GGGATCGAGTGTGTAGGCTT
PRIMER_INTERNAL_1_SEQUENCE=TATTTCCCAGAGGGAAAGCC
PRIMER_LEFT_1=35,20
PRIMER_RIGHT_1=118,20
PRIMER_INTERNAL_1=59,20
PRIMER_LEFT_1_TM=60.362
PRIMER_RIGHT_1_TM=59.166
PRIMER_INTERNAL_1_TM=60.391
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=9.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=1.382055
PRIMER_LEFT_2_PENALTY=0.361767
PRIMER_RIGHT_2_PENALTY=1.020289
PRIMER_INTERNAL_2_PENALTY=0.390581
PRIMER_LEFT_2_SEQUENCE=GCTCAAAACCCCACAATGAC
PRIMER_RIGHT_2_SEQUENCE=AAACAACAGGGATCGAGTGTG
PRIMER_INTERNAL_2_SEQUENCE=TATTTCCCAGAGGGAAAGCC
PRIMER_LEFT_2=35,20
PRIMER_RIGHT_2=126,21
PRIMER_INTERNAL_2=59,20
PRIMER_LEFT_2_TM=60.362
PRIMER_RIGHT_2_TM=60.020
PRIMER_INTERNAL_2_TM=60.391
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=9.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=1.491207
PRIMER_LEFT_3_PENALTY=0.813021
PRIMER_RIGHT_3_PENALTY=0.678186
PRIMER_INTERNAL_3_PENALTY=0.390581
PRIMER_LEFT_3_SEQUENCE=ACTTCTGTGCTCAAAACCCC
PRIMER_RIGHT_3_SEQUENCE=CAGGGATCGAGTGTGTAGGC
PRIMER_INTERNAL_3_SEQUENCE=TATTTCCCAGAGGGAAAGCC
PRIMER_LEFT_3=27,20
PRIMER_RIGHT_3=120,20
PRIMER_INTERNAL_3=59,20
PRIMER_LEFT_3_TM=59.187
PRIMER_RIGHT_3_TM=60.678
PRIMER_INTERNAL_3_TM=60.391
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=9.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.6000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=1.507911
PRIMER_LEFT_4_PENALTY=0.361767
PRIMER_RIGHT_4_PENALTY=1.146144
PRIMER_INTERNAL_4_PENALTY=0.390581
PRIMER_LEFT_4_SEQUENCE=GCTCAAAACCCCACAATGAC
PRIMER_RIGHT_4_SEQUENCE=AGGGATCGAGTGTGTAGGCTT
PRIMER_INTERNAL_4_SEQUENCE=TATTTCCCAGAGGGAAAGCC
PRIMER_LEFT_4=35,20
PRIMER_RIGHT_4=119,21
PRIMER_INTERNAL_4=59,20
PRIMER_LEFT_4_TM=60.362
PRIMER_RIGHT_4_TM=60.146
PRIMER_INTERNAL_4_TM=60.391
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=9.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH486
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCAAAAAACTTGATTTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
SEQUENCE_INCLUDED_REGION=36,15
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH488
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGACATTGTTCTTACCCTAGATCATTGTGATTTACAGAGAAAGAGCAATATAAGATCAATTGTACAAAACTCAAATGTTAGGAATTTCTCC
SEQUENCE_INCLUDED_REGION=36,94
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=12.602333
PRIMER_LEFT_0_PENALTY=5.889990
PRIMER_RIGHT_0_PENALTY=6.712343
PRIMER_INTERNAL_0_PENALTY=8.906203
PRIMER_LEFT_0_SEQUENCE=TTGTTCTTACCCTAGATCATTGTGA
PRIMER_RIGHT_0_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT
PRIMER_INTERNAL_0_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
PRIMER_LEFT_0=44,25
PRIMER_RIGHT_0=129,25
PRIMER_INTERNAL_0=72,26
PRIMER_LEFT_0_TM=59.110
PRIMER_RIGHT_0_TM=58.288
PRIMER_INTERNAL_0_TM=57.094
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=32.000
PRIMER_INTERNAL_0_GC_PERCENT=30.769
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=12.832894
PRIMER_LEFT_1_PENALTY=5.889990
PRIMER_RIGHT_1_PENALTY=6.942904
PRIMER_INTERNAL_1_PENALTY=8.906203
PRIMER_LEFT_1_SEQUENCE=TTGTTCTTACCCTAGATCATTGTGA
PRIMER_RIGHT_1_SEQUENCE=GGAGAAATTCCTAACATTTGAGTT
PRIMER_INTERNAL_1_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
PRIMER_LEFT_1=44,25
PRIMER_RIGHT_1=129,24
PRIMER_INTERNAL_1=72,26
PRIMER_LEFT_1_TM=59.110
PRIMER_RIGHT_1_TM=57.057
PRIMER_INTERNAL_1_TM=57.094
PRIMER_LEFT_1_GC_PERCENT=36.000
PRIMER_RIGHT_1_GC_PERCENT=33.333
PRIMER_INTERNAL_1_GC_PERCENT=30.769
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=86
PRIMER_PAIR_2_PENALTY=12.884753
PRIMER_LEFT_2_PENALTY=6.172410
PRIMER_RIGHT_2_PENALTY=6.712343
PRIMER_INTERNAL_2_PENALTY=8.906203
PRIMER_LEFT_2_SEQUENCE=TGTTCTTACCCTAGATCATTGTGA
PRIMER_RIGHT_2_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT
PRIMER_INTERNAL_2_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
PRIMER_LEFT_2=45,24
PRIMER_RIGHT_2=129,25
PRIMER_INTERNAL_2=72,26
PRIMER_LEFT_2_TM=57.828
PRIMER_RIGHT_2_TM=58.288
PRIMER_INTERNAL_2_TM=57.094
PRIMER_LEFT_2_GC_PERCENT=37.500
PRIMER_RIGHT_2_GC_PERCENT=32.000
PRIMER_INTERNAL_2_GC_PERCENT=30.769
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=13.115314
PRIMER_LEFT_3_PENALTY=6.172410
PRIMER_RIGHT_3_PENALTY=6.942904
PRIMER_INTERNAL_3_PENALTY=8.906203
PRIMER_LEFT_3_SEQUENCE=TGTTCTTACCCTAGATCATTGTGA
PRIMER_RIGHT_3_SEQUENCE=GGAGAAATTCCTAACATTTGAGTT
PRIMER_INTERNAL_3_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
PRIMER_LEFT_3=45,24
PRIMER_RIGHT_3=129,24
PRIMER_INTERNAL_3=72,26
PRIMER_LEFT_3_TM=57.828
PRIMER_RIGHT_3_TM=57.057
PRIMER_INTERNAL_3_TM=57.094
PRIMER_LEFT_3_GC_PERCENT=37.500
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=13.302043
PRIMER_LEFT_4_PENALTY=6.589700
PRIMER_RIGHT_4_PENALTY=6.712343
PRIMER_INTERNAL_4_PENALTY=8.906203
PRIMER_LEFT_4_SEQUENCE=TTGTTCTTACCCTAGATCATTGTG
PRIMER_RIGHT_4_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT
PRIMER_INTERNAL_4_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA
PRIMER_LEFT_4=44,24
PRIMER_RIGHT_4=129,25
PRIMER_INTERNAL_4=72,26
PRIMER_LEFT_4_TM=57.410
PRIMER_RIGHT_4_TM=58.288
PRIMER_INTERNAL_4_TM=57.094
PRIMER_LEFT_4_GC_PERCENT=37.500
PRIMER_RIGHT_4_GC_PERCENT=32.000
PRIMER_INTERNAL_4_GC_PERCENT=30.769
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH490
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTCAAATTATGCATACTATATGCAGACAACTTTGTAAACAAATAAAAAACAGGAAAAGATGGGTAAGTTATACATTAAAATGTTAACAATAATTATCCTTGAGTNNTNGGACCTTGTAATTTTTACGTTTATATATTTNCTTAATATTGAAAATTCATACTCTTGCAGTNNAAACTATGTTTTGAAGCCCTGTCACATAGATGAGAATTAGACTTATTTTCTATGACTCTGGAAGCAGAATAAG
SEQUENCE_INCLUDED_REGION=36,250
P3_COMMENT=CL is ATAAAAAACAGGAAAAGATGGG
P3_COMMENT=CR is TTCTCATCTATGTGACAGGGC
P3_COMMENT=CM is ACCCATCTTTTCCTGTTTTTTAT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.436790
PRIMER_LEFT_0_PENALTY=7.158805
PRIMER_RIGHT_0_PENALTY=0.277985
PRIMER_INTERNAL_0_PENALTY=8.721666
PRIMER_LEFT_0_SEQUENCE=CAGGAAAAGATGGGTAAGTTATACA
PRIMER_RIGHT_0_SEQUENCE=TGTGACAGGGCTTCAAAACA
PRIMER_INTERNAL_0_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
PRIMER_LEFT_0=91,25
PRIMER_RIGHT_0=238,20
PRIMER_INTERNAL_0=184,26
PRIMER_LEFT_0_TM=57.841
PRIMER_RIGHT_0_TM=60.278
PRIMER_INTERNAL_0_TM=57.278
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=26.923
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=148
PRIMER_PAIR_1_PENALTY=7.980162
PRIMER_LEFT_1_PENALTY=5.619366
PRIMER_RIGHT_1_PENALTY=2.360796
PRIMER_INTERNAL_1_PENALTY=8.721666
PRIMER_LEFT_1_SEQUENCE=AAACAGGAAAAGATGGGTAAGTT
PRIMER_RIGHT_1_SEQUENCE=GTGACAGGGCTTCAAAACAT
PRIMER_INTERNAL_1_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
PRIMER_LEFT_1=88,23
PRIMER_RIGHT_1=237,20
PRIMER_INTERNAL_1=184,26
PRIMER_LEFT_1_TM=57.381
PRIMER_RIGHT_1_TM=57.639
PRIMER_INTERNAL_1_TM=57.278
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=26.923
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=150
PRIMER_PAIR_2_PENALTY=8.435604
PRIMER_LEFT_2_PENALTY=5.387193
PRIMER_RIGHT_2_PENALTY=3.048411
PRIMER_INTERNAL_2_PENALTY=8.721666
PRIMER_LEFT_2_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT
PRIMER_RIGHT_2_SEQUENCE=TGACAGGGCTTCAAAACATAGTT
PRIMER_INTERNAL_2_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
PRIMER_LEFT_2=87,24
PRIMER_RIGHT_2=236,23
PRIMER_INTERNAL_2=184,26
PRIMER_LEFT_2_TM=58.613
PRIMER_RIGHT_2_TM=60.048
PRIMER_INTERNAL_2_TM=57.278
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_INTERNAL_2_GC_PERCENT=26.923
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=150
PRIMER_PAIR_3_PENALTY=8.494563
PRIMER_LEFT_3_PENALTY=5.387193
PRIMER_RIGHT_3_PENALTY=3.107370
PRIMER_INTERNAL_3_PENALTY=8.721666
PRIMER_LEFT_3_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT
PRIMER_RIGHT_3_SEQUENCE=TGACAGGGCTTCAAAACATAG
PRIMER_INTERNAL_3_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
PRIMER_LEFT_3=87,24
PRIMER_RIGHT_3=236,21
PRIMER_INTERNAL_3=184,26
PRIMER_LEFT_3_TM=58.613
PRIMER_RIGHT_3_TM=57.893
PRIMER_INTERNAL_3_TM=57.278
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=26.923
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=150
PRIMER_PAIR_4_PENALTY=8.619905
PRIMER_LEFT_4_PENALTY=5.387193
PRIMER_RIGHT_4_PENALTY=3.232712
PRIMER_INTERNAL_4_PENALTY=8.721666
PRIMER_LEFT_4_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT
PRIMER_RIGHT_4_SEQUENCE=TGACAGGGCTTCAAAACATAGT
PRIMER_INTERNAL_4_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG
PRIMER_LEFT_4=87,24
PRIMER_RIGHT_4=236,22
PRIMER_INTERNAL_4=184,26
PRIMER_LEFT_4_TM=58.613
PRIMER_RIGHT_4_TM=58.767
PRIMER_INTERNAL_4_TM=57.278
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_INTERNAL_4_GC_PERCENT=26.923
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=150
=
SEQUENCE_ID=MH504
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACCTGGTTTGAAATTTGGGGGTCTGAGCCTCCACTGTNGTNTTACCTAGCAGGCTTCCCGGACTTAGTCTTACATGCACATGCACACCGTAAAAAAGAGACCAAAGAAAAATCTGCAAGANTTGCTCAAATAACACAAGACGGGGATGAACTGGATCTACAGGTCTAGAGTNNTTCAGAACTAAAGACGCCTGTCACACGGCTTAAGCAAGAGCAGGAAGGGGCTGCCAGGCCTCTGGGAAGACCTCCCTGGGGAGNGAGCAGGCAGACAAATGGCCCGGATAGAGATCCCCCGNTTACGTGATGTTTTCCTTCCTAAAGATAGCGC
SEQUENCE_INCLUDED_REGION=36,331
P3_COMMENT=CL is AACCTGGTTTGAAATTTGGG
P3_COMMENT=CR is TTAAGCCGTGTGACAGGC
P3_COMMENT=CM is TTACCTAGCAGGCTTCCCG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.302471
PRIMER_LEFT_0_PENALTY=0.050165
PRIMER_RIGHT_0_PENALTY=0.252306
PRIMER_INTERNAL_0_PENALTY=1.230410
PRIMER_LEFT_0_SEQUENCE=TGTCACACGGCTTAAGCAAG
PRIMER_RIGHT_0_SEQUENCE=GATCTCTATCCGGGCCATTT
PRIMER_INTERNAL_0_SEQUENCE=CTCTGGGAAGACCTCCCTG
PRIMER_LEFT_0=231,20
PRIMER_RIGHT_0=328,20
PRIMER_INTERNAL_0=272,19
PRIMER_LEFT_0_TM=60.050
PRIMER_RIGHT_0_TM=60.252
PRIMER_INTERNAL_0_TM=59.770
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=0.302471
PRIMER_LEFT_1_PENALTY=0.050165
PRIMER_RIGHT_1_PENALTY=0.252306
PRIMER_INTERNAL_1_PENALTY=1.230410
PRIMER_LEFT_1_SEQUENCE=CTGTCACACGGCTTAAGCAA
PRIMER_RIGHT_1_SEQUENCE=GATCTCTATCCGGGCCATTT
PRIMER_INTERNAL_1_SEQUENCE=CTCTGGGAAGACCTCCCTG
PRIMER_LEFT_1=230,20
PRIMER_RIGHT_1=328,20
PRIMER_INTERNAL_1=272,19
PRIMER_LEFT_1_TM=60.050
PRIMER_RIGHT_1_TM=60.252
PRIMER_INTERNAL_1_TM=59.770
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=63.158
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=0.443054
PRIMER_LEFT_2_PENALTY=0.173087
PRIMER_RIGHT_2_PENALTY=0.269968
PRIMER_INTERNAL_2_PENALTY=2.052187
PRIMER_LEFT_2_SEQUENCE=AACACAAGACGGGGATGAAC
PRIMER_RIGHT_2_SEQUENCE=TTCCTGCTCTTGCTTAAGCC
PRIMER_INTERNAL_2_SEQUENCE=TCAGAACTAAAGACGCCTGTCA
PRIMER_LEFT_2=171,20
PRIMER_RIGHT_2=258,20
PRIMER_INTERNAL_2=214,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=59.730
PRIMER_INTERNAL_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=0.460268
PRIMER_LEFT_3_PENALTY=0.287182
PRIMER_RIGHT_3_PENALTY=0.173087
PRIMER_INTERNAL_3_PENALTY=0.566194
PRIMER_LEFT_3_SEQUENCE=GGCTTCCCGGACTTAGTCTT
PRIMER_RIGHT_3_SEQUENCE=GTTCATCCCCGTCTTGTGTT
PRIMER_INTERNAL_3_SEQUENCE=GCACATGCACACCGTAAAAA
PRIMER_LEFT_3=91,20
PRIMER_RIGHT_3=190,20
PRIMER_INTERNAL_3=115,20
PRIMER_LEFT_3_TM=59.713
PRIMER_RIGHT_3_TM=59.827
PRIMER_INTERNAL_3_TM=60.566
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=0.522274
PRIMER_LEFT_4_PENALTY=0.269968
PRIMER_RIGHT_4_PENALTY=0.252306
PRIMER_INTERNAL_4_PENALTY=1.230410
PRIMER_LEFT_4_SEQUENCE=GGCTTAAGCAAGAGCAGGAA
PRIMER_RIGHT_4_SEQUENCE=GATCTCTATCCGGGCCATTT
PRIMER_INTERNAL_4_SEQUENCE=CTCTGGGAAGACCTCCCTG
PRIMER_LEFT_4=239,20
PRIMER_RIGHT_4=328,20
PRIMER_INTERNAL_4=272,19
PRIMER_LEFT_4_TM=59.730
PRIMER_RIGHT_4_TM=60.252
PRIMER_INTERNAL_4_TM=59.770
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=63.158
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=90
=
SEQUENCE_ID=MH509
SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCCTGGGAAGGAGGCTGAGACTCACATTGTNAGCTTTCACTCACTAAAGAGAGTCTTGCTTGAGGGGCTTGGACAAACAGGAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
SEQUENCE_INCLUDED_REGION=35,86
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.561777
PRIMER_LEFT_0_PENALTY=0.921348
PRIMER_RIGHT_0_PENALTY=1.640429
PRIMER_INTERNAL_0_PENALTY=6.525910
PRIMER_LEFT_0_SEQUENCE=CTGGGAAGGAGGCTGAGACT
PRIMER_RIGHT_0_SEQUENCE=CTGTTTGTCCAAGCCCCTC
PRIMER_INTERNAL_0_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
PRIMER_LEFT_0=41,20
PRIMER_RIGHT_0=118,19
PRIMER_INTERNAL_0=73,25
PRIMER_LEFT_0_TM=60.921
PRIMER_RIGHT_0_TM=60.640
PRIMER_INTERNAL_0_TM=58.474
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=2.663352
PRIMER_LEFT_1_PENALTY=1.022923
PRIMER_RIGHT_1_PENALTY=1.640429
PRIMER_INTERNAL_1_PENALTY=6.525910
PRIMER_LEFT_1_SEQUENCE=GGAAGGAGGCTGAGACTCAC
PRIMER_RIGHT_1_SEQUENCE=CTGTTTGTCCAAGCCCCTC
PRIMER_INTERNAL_1_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
PRIMER_LEFT_1=44,20
PRIMER_RIGHT_1=118,19
PRIMER_INTERNAL_1=73,25
PRIMER_LEFT_1_TM=58.977
PRIMER_RIGHT_1_TM=60.640
PRIMER_INTERNAL_1_TM=58.474
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=2.711523
PRIMER_LEFT_2_PENALTY=1.071094
PRIMER_RIGHT_2_PENALTY=1.640429
PRIMER_INTERNAL_2_PENALTY=6.525910
PRIMER_LEFT_2_SEQUENCE=TGGGAAGGAGGCTGAGACT
PRIMER_RIGHT_2_SEQUENCE=CTGTTTGTCCAAGCCCCTC
PRIMER_INTERNAL_2_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
PRIMER_LEFT_2=42,19
PRIMER_RIGHT_2=118,19
PRIMER_INTERNAL_2=73,25
PRIMER_LEFT_2_TM=59.929
PRIMER_RIGHT_2_TM=60.640
PRIMER_INTERNAL_2_TM=58.474
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=2.711523
PRIMER_LEFT_3_PENALTY=1.071094
PRIMER_RIGHT_3_PENALTY=1.640429
PRIMER_INTERNAL_3_PENALTY=6.525910
PRIMER_LEFT_3_SEQUENCE=CTGGGAAGGAGGCTGAGAC
PRIMER_RIGHT_3_SEQUENCE=CTGTTTGTCCAAGCCCCTC
PRIMER_INTERNAL_3_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC
PRIMER_LEFT_3=41,19
PRIMER_RIGHT_3=118,19
PRIMER_INTERNAL_3=73,25
PRIMER_LEFT_3_TM=59.929
PRIMER_RIGHT_3_TM=60.640
PRIMER_INTERNAL_3_TM=58.474
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=2.862491
PRIMER_LEFT_4_PENALTY=0.921348
PRIMER_RIGHT_4_PENALTY=1.941143
PRIMER_INTERNAL_4_PENALTY=6.645031
PRIMER_LEFT_4_SEQUENCE=CTGGGAAGGAGGCTGAGACT
PRIMER_RIGHT_4_SEQUENCE=GTTTGTCCAAGCCCCTCAAG
PRIMER_INTERNAL_4_SEQUENCE=GCTTTCACTCACTAAAGAGAGTCTTG
PRIMER_LEFT_4=41,20
PRIMER_RIGHT_4=116,20
PRIMER_INTERNAL_4=71,26
PRIMER_LEFT_4_TM=60.921
PRIMER_RIGHT_4_TM=61.941
PRIMER_INTERNAL_4_TM=59.355
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=42.308
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH512
SEQUENCE_TEMPLATE=CCTGCATGTGGCACCCACCTGAGGCCAGAGACATCTGGAACTCTAAGATTGGGCCCCACAGGAGGATGCTGTGTGGGTTCTGTGGATCCCAGCCACTCCAAAGAGGACACTCTCGGCAGAGGTTCT
SEQUENCE_INCLUDED_REGION=0,126
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.103757
PRIMER_LEFT_0_PENALTY=0.096820
PRIMER_RIGHT_0_PENALTY=0.006937
PRIMER_INTERNAL_0_PENALTY=0.119809
PRIMER_LEFT_0_SEQUENCE=GAACTCTAAGATTGGGCCCC
PRIMER_RIGHT_0_SEQUENCE=GAACCTCTGCCGAGAGTGTC
PRIMER_INTERNAL_0_SEQUENCE=GAGGATGCTGTGTGGGTTCT
PRIMER_LEFT_0=37,20
PRIMER_RIGHT_0=124,20
PRIMER_INTERNAL_0=61,20
PRIMER_LEFT_0_TM=59.903
PRIMER_RIGHT_0_TM=59.993
PRIMER_INTERNAL_0_TM=60.120
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.103757
PRIMER_LEFT_1_PENALTY=0.096820
PRIMER_RIGHT_1_PENALTY=0.006937
PRIMER_INTERNAL_1_PENALTY=0.119809
PRIMER_LEFT_1_SEQUENCE=GGAACTCTAAGATTGGGCCC
PRIMER_RIGHT_1_SEQUENCE=GAACCTCTGCCGAGAGTGTC
PRIMER_INTERNAL_1_SEQUENCE=GAGGATGCTGTGTGGGTTCT
PRIMER_LEFT_1=36,20
PRIMER_RIGHT_1=124,20
PRIMER_INTERNAL_1=61,20
PRIMER_LEFT_1_TM=59.903
PRIMER_RIGHT_1_TM=59.993
PRIMER_INTERNAL_1_TM=60.120
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=89
PRIMER_PAIR_2_PENALTY=0.232151
PRIMER_LEFT_2_PENALTY=0.096820
PRIMER_RIGHT_2_PENALTY=0.135331
PRIMER_INTERNAL_2_PENALTY=0.119809
PRIMER_LEFT_2_SEQUENCE=GAACTCTAAGATTGGGCCCC
PRIMER_RIGHT_2_SEQUENCE=TCTGCCGAGAGTGTCCTCTT
PRIMER_INTERNAL_2_SEQUENCE=GAGGATGCTGTGTGGGTTCT
PRIMER_LEFT_2=37,20
PRIMER_RIGHT_2=119,20
PRIMER_INTERNAL_2=61,20
PRIMER_LEFT_2_TM=59.903
PRIMER_RIGHT_2_TM=60.135
PRIMER_INTERNAL_2_TM=60.120
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.232151
PRIMER_LEFT_3_PENALTY=0.096820
PRIMER_RIGHT_3_PENALTY=0.135331
PRIMER_INTERNAL_3_PENALTY=0.119809
PRIMER_LEFT_3_SEQUENCE=GGAACTCTAAGATTGGGCCC
PRIMER_RIGHT_3_SEQUENCE=TCTGCCGAGAGTGTCCTCTT
PRIMER_INTERNAL_3_SEQUENCE=GAGGATGCTGTGTGGGTTCT
PRIMER_LEFT_3=36,20
PRIMER_RIGHT_3=119,20
PRIMER_INTERNAL_3=61,20
PRIMER_LEFT_3_TM=59.903
PRIMER_RIGHT_3_TM=60.135
PRIMER_INTERNAL_3_TM=60.120
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=0.334471
PRIMER_LEFT_4_PENALTY=0.327533
PRIMER_RIGHT_4_PENALTY=0.006937
PRIMER_INTERNAL_4_PENALTY=0.119809
PRIMER_LEFT_4_SEQUENCE=ACTCTAAGATTGGGCCCCAC
PRIMER_RIGHT_4_SEQUENCE=GAACCTCTGCCGAGAGTGTC
PRIMER_INTERNAL_4_SEQUENCE=GAGGATGCTGTGTGGGTTCT
PRIMER_LEFT_4=39,20
PRIMER_RIGHT_4=124,20
PRIMER_INTERNAL_4=61,20
PRIMER_LEFT_4_TM=60.328
PRIMER_RIGHT_4_TM=59.993
PRIMER_INTERNAL_4_TM=60.120
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH516
SEQUENCE_TEMPLATE=TAGGGTCCTTCTCCCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG
SEQUENCE_INCLUDED_REGION=0,17
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH519
SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCGTGGCACCCGGCAGCCTCCCAGGAGACAGCCAGGCAGGAGTGATGATCAGGATTGTGTTCAGTTTTCAGCATGTTTCCATGTACCCAAAAGTTAAGAGTCCCATCTACCTCTATAGTAAAACAAAATGATAGTAAATACATTCCATAAAAATGTTTTTCAAGCCATCAGTTTTGTTTCCGGCCTTATCTGTCAGTATTCCTTTCAGGCACATTCTGAGATTCACAACTGTGTTCTTTAAACAGCCAAAATTCCCTCTTCTCCAGCATGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAG
SEQUENCE_INCLUDED_REGION=36,237
P3_COMMENT=CL is TTCAGTTTTCAGCATGTTTCC
P3_COMMENT=CR is CTCAGAATGTGCCTGAAAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.262193
PRIMER_LEFT_0_PENALTY=2.513402
PRIMER_RIGHT_0_PENALTY=0.748791
PRIMER_INTERNAL_0_PENALTY=0.222782
PRIMER_LEFT_0_SEQUENCE=AGCCTCCCAGGAGACAGC
PRIMER_RIGHT_0_SEQUENCE=TTGGGTACATGGAAACATGC
PRIMER_INTERNAL_0_SEQUENCE=AGGCAGGAGTGATGATCAGG
PRIMER_LEFT_0=52,18
PRIMER_RIGHT_0=127,20
PRIMER_INTERNAL_0=71,20
PRIMER_LEFT_0_TM=60.513
PRIMER_RIGHT_0_TM=59.251
PRIMER_INTERNAL_0_TM=60.223
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=3.279658
PRIMER_LEFT_1_PENALTY=2.530866
PRIMER_RIGHT_1_PENALTY=0.748791
PRIMER_INTERNAL_1_PENALTY=0.222782
PRIMER_LEFT_1_SEQUENCE=CAGCCTCCCAGGAGACAG
PRIMER_RIGHT_1_SEQUENCE=TTGGGTACATGGAAACATGC
PRIMER_INTERNAL_1_SEQUENCE=AGGCAGGAGTGATGATCAGG
PRIMER_LEFT_1=51,18
PRIMER_RIGHT_1=127,20
PRIMER_INTERNAL_1=71,20
PRIMER_LEFT_1_TM=59.469
PRIMER_RIGHT_1_TM=59.251
PRIMER_INTERNAL_1_TM=60.223
PRIMER_LEFT_1_GC_PERCENT=66.667
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=3.334690
PRIMER_LEFT_2_PENALTY=0.222782
PRIMER_RIGHT_2_PENALTY=3.111908
PRIMER_INTERNAL_2_PENALTY=1.692817
PRIMER_LEFT_2_SEQUENCE=AGGCAGGAGTGATGATCAGG
PRIMER_RIGHT_2_SEQUENCE=AGATGGGACTCTTAACTTTTGGG
PRIMER_INTERNAL_2_SEQUENCE=TCAGTTTTCAGCATGTTTCCA
PRIMER_LEFT_2=71,20
PRIMER_RIGHT_2=145,23
PRIMER_INTERNAL_2=98,21
PRIMER_LEFT_2_TM=60.223
PRIMER_RIGHT_2_TM=59.888
PRIMER_INTERNAL_2_TM=59.307
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_INTERNAL_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=3.632149
PRIMER_LEFT_3_PENALTY=0.633068
PRIMER_RIGHT_3_PENALTY=2.999081
PRIMER_INTERNAL_3_PENALTY=1.692817
PRIMER_LEFT_3_SEQUENCE=CAGGCAGGAGTGATGATCAG
PRIMER_RIGHT_3_SEQUENCE=GATGGGACTCTTAACTTTTGGG
PRIMER_INTERNAL_3_SEQUENCE=TCAGTTTTCAGCATGTTTCCA
PRIMER_LEFT_3=70,20
PRIMER_RIGHT_3=144,22
PRIMER_INTERNAL_3=98,21
PRIMER_LEFT_3_TM=59.367
PRIMER_RIGHT_3_TM=59.001
PRIMER_INTERNAL_3_TM=59.307
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=3.663301
PRIMER_LEFT_4_PENALTY=2.513402
PRIMER_RIGHT_4_PENALTY=1.149900
PRIMER_INTERNAL_4_PENALTY=0.222782
PRIMER_LEFT_4_SEQUENCE=AGCCTCCCAGGAGACAGC
PRIMER_RIGHT_4_SEQUENCE=TGGGTACATGGAAACATGCT
PRIMER_INTERNAL_4_SEQUENCE=AGGCAGGAGTGATGATCAGG
PRIMER_LEFT_4=52,18
PRIMER_RIGHT_4=126,20
PRIMER_INTERNAL_4=71,20
PRIMER_LEFT_4_TM=60.513
PRIMER_RIGHT_4_TM=58.850
PRIMER_INTERNAL_4_TM=60.223
PRIMER_LEFT_4_GC_PERCENT=66.667
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH521
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTAAAAAGATATCCTCAAAGAATNTAATCTAGATCCTAGAATTTAGGATAATACTTAAAAATATGTATTTTAGATATACCTTCTCTACGTTATATTATTAAAATAATGTGTTTTCAATTTCCACATTGACAATAACTTTTCTGTAATAACTTGGTTTTTGAACCTATAACAAATACACATGAATATAGGGAGTTAATCATTTGGTAACCAATAAATGCTCCACAGACTTCCCATATTATAATAAACCTCAGGTAATATCTGATTCTTCAGAAATTCACCCA
SEQUENCE_INCLUDED_REGION=33,284
P3_COMMENT=CL is AAATAATGTGTTTTCAATTTCCACA
P3_COMMENT=CR is TGGGTGAATTTCTGAAGAATCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.091192
PRIMER_LEFT_0_PENALTY=2.637146
PRIMER_RIGHT_0_PENALTY=2.454047
PRIMER_INTERNAL_0_PENALTY=8.235014
PRIMER_LEFT_0_SEQUENCE=TTGGTAACCAATAAATGCTCCA
PRIMER_RIGHT_0_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
PRIMER_INTERNAL_0_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
PRIMER_LEFT_0=236,22
PRIMER_RIGHT_0=316,22
PRIMER_INTERNAL_0=262,26
PRIMER_LEFT_0_TM=59.363
PRIMER_RIGHT_0_TM=59.546
PRIMER_INTERNAL_0_TM=57.765
PRIMER_LEFT_0_GC_PERCENT=36.364
PRIMER_RIGHT_0_GC_PERCENT=36.364
PRIMER_INTERNAL_0_GC_PERCENT=34.615
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=7.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=5.422938
PRIMER_LEFT_1_PENALTY=2.968891
PRIMER_RIGHT_1_PENALTY=2.454047
PRIMER_INTERNAL_1_PENALTY=8.235014
PRIMER_LEFT_1_SEQUENCE=TGGTAACCAATAAATGCTCCA
PRIMER_RIGHT_1_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
PRIMER_INTERNAL_1_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
PRIMER_LEFT_1=237,21
PRIMER_RIGHT_1=316,22
PRIMER_INTERNAL_1=262,26
PRIMER_LEFT_1_TM=58.031
PRIMER_RIGHT_1_TM=59.546
PRIMER_INTERNAL_1_TM=57.765
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=36.364
PRIMER_INTERNAL_1_GC_PERCENT=34.615
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=5.575532
PRIMER_LEFT_2_PENALTY=3.121486
PRIMER_RIGHT_2_PENALTY=2.454047
PRIMER_INTERNAL_2_PENALTY=8.235014
PRIMER_LEFT_2_SEQUENCE=TTGGTAACCAATAAATGCTCCAC
PRIMER_RIGHT_2_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
PRIMER_INTERNAL_2_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
PRIMER_LEFT_2=236,23
PRIMER_RIGHT_2=316,22
PRIMER_INTERNAL_2=262,26
PRIMER_LEFT_2_TM=60.121
PRIMER_RIGHT_2_TM=59.546
PRIMER_INTERNAL_2_TM=57.765
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=36.364
PRIMER_INTERNAL_2_GC_PERCENT=34.615
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=10.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=7.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=5.576335
PRIMER_LEFT_3_PENALTY=3.122288
PRIMER_RIGHT_3_PENALTY=2.454047
PRIMER_INTERNAL_3_PENALTY=8.235014
PRIMER_LEFT_3_SEQUENCE=GGTAACCAATAAATGCTCCACA
PRIMER_RIGHT_3_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
PRIMER_INTERNAL_3_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
PRIMER_LEFT_3=238,22
PRIMER_RIGHT_3=316,22
PRIMER_INTERNAL_3=262,26
PRIMER_LEFT_3_TM=58.878
PRIMER_RIGHT_3_TM=59.546
PRIMER_INTERNAL_3_TM=57.765
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=36.364
PRIMER_INTERNAL_3_GC_PERCENT=34.615
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=7.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=5.576335
PRIMER_LEFT_4_PENALTY=3.122288
PRIMER_RIGHT_4_PENALTY=2.454047
PRIMER_INTERNAL_4_PENALTY=8.235014
PRIMER_LEFT_4_SEQUENCE=TGGTAACCAATAAATGCTCCAC
PRIMER_RIGHT_4_SEQUENCE=TGGGTGAATTTCTGAAGAATCA
PRIMER_INTERNAL_4_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG
PRIMER_LEFT_4=237,22
PRIMER_RIGHT_4=316,22
PRIMER_INTERNAL_4=262,26
PRIMER_LEFT_4_TM=58.878
PRIMER_RIGHT_4_TM=59.546
PRIMER_INTERNAL_4_TM=57.765
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=36.364
PRIMER_INTERNAL_4_GC_PERCENT=34.615
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=7.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH522
SEQUENCE_TEMPLATE=AGAATTTTAGTATTTTCTTTACTTTCAAATTTTCTGTCTCTCTATATTCATGGAATTACTAATGATAATTATTTAATACTGTCATCAGCGATCTCTATTATTCAGATCATTTATGTACCTATTCCTAAGATGTGTTTTTATGTTTCTTTTTACTGTCCAGTCATATGATCTTTTCTATTGACATGTCTTGTAATTTTNAAATNATTTTCTAGATAATGTTGTATAAATCATTCAAAGGCTTGAGATTATGACATCATCTGTGAGAGAATATTTACA
SEQUENCE_INCLUDED_REGION=0,276
P3_COMMENT=CL is ATTTTCTTTACTTTCAAATTTTCTG
P3_COMMENT=CR is ACAGATGATGTCATAATCTCAAGC
P3_COMMENT=CM is ATTTAATACTGTCATCAGCGATCTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=12.050740
PRIMER_LEFT_0_PENALTY=5.128157
PRIMER_RIGHT_0_PENALTY=6.922583
PRIMER_INTERNAL_0_PENALTY=9.885775
PRIMER_LEFT_0_SEQUENCE=TGTCATCAGCGATCTCTATTATTCA
PRIMER_RIGHT_0_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT
PRIMER_INTERNAL_0_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
PRIMER_LEFT_0=79,25
PRIMER_RIGHT_0=162,24
PRIMER_INTERNAL_0=112,27
PRIMER_LEFT_0_TM=60.128
PRIMER_RIGHT_0_TM=57.077
PRIMER_INTERNAL_0_TM=57.114
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=33.333
PRIMER_INTERNAL_0_GC_PERCENT=29.630
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=12.050740
PRIMER_LEFT_1_PENALTY=5.128157
PRIMER_RIGHT_1_PENALTY=6.922583
PRIMER_INTERNAL_1_PENALTY=9.885775
PRIMER_LEFT_1_SEQUENCE=TGTCATCAGCGATCTCTATTATTCA
PRIMER_RIGHT_1_SEQUENCE=ATGACTGGACAGTAAAAAGAAACA
PRIMER_INTERNAL_1_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
PRIMER_LEFT_1=79,25
PRIMER_RIGHT_1=163,24
PRIMER_INTERNAL_1=112,27
PRIMER_LEFT_1_TM=60.128
PRIMER_RIGHT_1_TM=57.077
PRIMER_INTERNAL_1_TM=57.114
PRIMER_LEFT_1_GC_PERCENT=36.000
PRIMER_RIGHT_1_GC_PERCENT=33.333
PRIMER_INTERNAL_1_GC_PERCENT=29.630
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=12.511201
PRIMER_LEFT_2_PENALTY=5.588618
PRIMER_RIGHT_2_PENALTY=6.922583
PRIMER_INTERNAL_2_PENALTY=9.885775
PRIMER_LEFT_2_SEQUENCE=TCAGCGATCTCTATTATTCAGATCA
PRIMER_RIGHT_2_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT
PRIMER_INTERNAL_2_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
PRIMER_LEFT_2=84,25
PRIMER_RIGHT_2=162,24
PRIMER_INTERNAL_2=112,27
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=57.077
PRIMER_INTERNAL_2_TM=57.114
PRIMER_LEFT_2_GC_PERCENT=36.000
PRIMER_RIGHT_2_GC_PERCENT=33.333
PRIMER_INTERNAL_2_GC_PERCENT=29.630
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=12.511201
PRIMER_LEFT_3_PENALTY=5.588618
PRIMER_RIGHT_3_PENALTY=6.922583
PRIMER_INTERNAL_3_PENALTY=9.885775
PRIMER_LEFT_3_SEQUENCE=TCAGCGATCTCTATTATTCAGATCA
PRIMER_RIGHT_3_SEQUENCE=ATGACTGGACAGTAAAAAGAAACA
PRIMER_INTERNAL_3_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
PRIMER_LEFT_3=84,25
PRIMER_RIGHT_3=163,24
PRIMER_INTERNAL_3=112,27
PRIMER_LEFT_3_TM=59.411
PRIMER_RIGHT_3_TM=57.077
PRIMER_INTERNAL_3_TM=57.114
PRIMER_LEFT_3_GC_PERCENT=36.000
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=29.630
PRIMER_LEFT_3_SELF_ANY=9.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=7.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=12.511201
PRIMER_LEFT_4_PENALTY=5.588618
PRIMER_RIGHT_4_PENALTY=6.922583
PRIMER_INTERNAL_4_PENALTY=9.885775
PRIMER_LEFT_4_SEQUENCE=TCATCAGCGATCTCTATTATTCAGA
PRIMER_RIGHT_4_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT
PRIMER_INTERNAL_4_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT
PRIMER_LEFT_4=81,25
PRIMER_RIGHT_4=162,24
PRIMER_INTERNAL_4=112,27
PRIMER_LEFT_4_TM=59.411
PRIMER_RIGHT_4_TM=57.077
PRIMER_INTERNAL_4_TM=57.114
PRIMER_LEFT_4_GC_PERCENT=36.000
PRIMER_RIGHT_4_GC_PERCENT=33.333
PRIMER_INTERNAL_4_GC_PERCENT=29.630
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH523
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTGTTTCTCGTCAGGGCAAGCCTTATTCACTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
SEQUENCE_INCLUDED_REGION=36,31
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH529
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCTGATAATTTTGTTTTCTGAAAGTAAATGACAATGTCCTTAATTTTTCGACAAGGGGCTAACTGGATTTGTACCTAGAGGTAGTGAAATNATCTGTGTAGGCATAGTACTGGATTCTAAGGAATNAGTNAACAGGACTTGTGAAAAATCTCCCATTTCTTGCATCTTATATCTTTGATACTTCCATTTATCATGCTGGGGGTAGAGAGTATGAGAAAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG
SEQUENCE_INCLUDED_REGION=36,188
P3_COMMENT=CL is TTCTGAAAGTAAATGACAATGTCC
P3_COMMENT=CR is AAGATGCAAGAAATGGGAGA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.306229
PRIMER_LEFT_0_PENALTY=1.282673
PRIMER_RIGHT_0_PENALTY=2.023556
PRIMER_INTERNAL_0_PENALTY=6.031815
PRIMER_LEFT_0_SEQUENCE=GGGGCTAACTGGATTTGTACC
PRIMER_RIGHT_0_SEQUENCE=GGGAGATTTTTCACAAGTCCTG
PRIMER_INTERNAL_0_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
PRIMER_LEFT_0=95,21
PRIMER_RIGHT_0=194,22
PRIMER_INTERNAL_0=141,24
PRIMER_LEFT_0_TM=59.717
PRIMER_RIGHT_0_TM=59.976
PRIMER_INTERNAL_0_TM=57.968
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=4.612671
PRIMER_LEFT_1_PENALTY=2.589115
PRIMER_RIGHT_1_PENALTY=2.023556
PRIMER_INTERNAL_1_PENALTY=6.031815
PRIMER_LEFT_1_SEQUENCE=GGGGCTAACTGGATTTGTACCT
PRIMER_RIGHT_1_SEQUENCE=GGGAGATTTTTCACAAGTCCTG
PRIMER_INTERNAL_1_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
PRIMER_LEFT_1=95,22
PRIMER_RIGHT_1=194,22
PRIMER_INTERNAL_1=141,24
PRIMER_LEFT_1_TM=60.589
PRIMER_RIGHT_1_TM=59.976
PRIMER_INTERNAL_1_TM=57.968
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=5.019667
PRIMER_LEFT_2_PENALTY=1.282673
PRIMER_RIGHT_2_PENALTY=3.736993
PRIMER_INTERNAL_2_PENALTY=6.031815
PRIMER_LEFT_2_SEQUENCE=GGGGCTAACTGGATTTGTACC
PRIMER_RIGHT_2_SEQUENCE=GGGAGATTTTTCACAAGTCCTGT
PRIMER_INTERNAL_2_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
PRIMER_LEFT_2=95,21
PRIMER_RIGHT_2=194,23
PRIMER_INTERNAL_2=141,24
PRIMER_LEFT_2_TM=59.717
PRIMER_RIGHT_2_TM=60.737
PRIMER_INTERNAL_2_TM=57.968
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=5.041827
PRIMER_LEFT_3_PENALTY=1.282673
PRIMER_RIGHT_3_PENALTY=3.759153
PRIMER_INTERNAL_3_PENALTY=6.031815
PRIMER_LEFT_3_SEQUENCE=GGGGCTAACTGGATTTGTACC
PRIMER_RIGHT_3_SEQUENCE=GGGAGATTTTTCACAAGTCCT
PRIMER_INTERNAL_3_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA
PRIMER_LEFT_3=95,21
PRIMER_RIGHT_3=194,21
PRIMER_INTERNAL_3=141,24
PRIMER_LEFT_3_TM=59.717
PRIMER_RIGHT_3_TM=57.241
PRIMER_INTERNAL_3_TM=57.968
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=5.288961
PRIMER_LEFT_4_PENALTY=4.006288
PRIMER_RIGHT_4_PENALTY=1.282673
PRIMER_INTERNAL_4_PENALTY=4.336347
PRIMER_LEFT_4_SEQUENCE=TGCCTGATAATTTTGTTTTCTGAA
PRIMER_RIGHT_4_SEQUENCE=GGTACAAATCCAGTTAGCCCC
PRIMER_INTERNAL_4_SEQUENCE=TGACAATGTCCTTAATTTTTCGA
PRIMER_LEFT_4=39,24
PRIMER_RIGHT_4=115,21
PRIMER_INTERNAL_4=69,23
PRIMER_LEFT_4_TM=60.006
PRIMER_RIGHT_4_TM=59.717
PRIMER_INTERNAL_4_TM=58.664
PRIMER_LEFT_4_GC_PERCENT=29.167
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=30.435
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH533
SEQUENCE_TEMPLATE=CTTACATTGAAGAGCCAGTAGCGTTTGATAACAAGTTAGAGANNNNAAGTNAATGATGCCTCCAAAAGTTTGATCTGAGAAACACAAAGGATGAAAAGTNAGATGGGAAGAAAGTGGTAGGAACAGACATTTTTNCTTGGTTAGTTAATTGGCTGTTTTGTGTGTGTGTNTNTNTGGTGGGTGGAGAGTNTTGTATGTAGGGGTTGAATAGACCCTCACTTCAGGGCATATTAAGATCAAGATTTCTATGCATCCCATGCTAGATATATACCCAGAAGAAAGGAAGTCTATTGAAAAGATATCTGCACTCCCATGCTTAT
SEQUENCE_INCLUDED_REGION=0,320
P3_COMMENT=CL is ACATTGAAGAGCCAGTAGCG
P3_COMMENT=CR is GCATGGGATGCATAGAAATC
P3_COMMENT=CM is AATGATGCCTCCAAAAGTTTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.817266
PRIMER_LEFT_0_PENALTY=1.984975
PRIMER_RIGHT_0_PENALTY=1.832292
PRIMER_INTERNAL_0_PENALTY=4.120932
PRIMER_LEFT_0_SEQUENCE=GATTTCTATGCATCCCATGCT
PRIMER_RIGHT_0_SEQUENCE=GCATGGGAGTGCAGATATCTT
PRIMER_INTERNAL_0_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
PRIMER_LEFT_0=240,21
PRIMER_RIGHT_0=315,21
PRIMER_INTERNAL_0=270,24
PRIMER_LEFT_0_TM=59.015
PRIMER_RIGHT_0_TM=59.168
PRIMER_INTERNAL_0_TM=60.121
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=10.00
PRIMER_PAIR_0_COMPL_END=8.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=3.874486
PRIMER_LEFT_1_PENALTY=2.042194
PRIMER_RIGHT_1_PENALTY=1.832292
PRIMER_INTERNAL_1_PENALTY=4.120932
PRIMER_LEFT_1_SEQUENCE=AGATTTCTATGCATCCCATGCT
PRIMER_RIGHT_1_SEQUENCE=GCATGGGAGTGCAGATATCTT
PRIMER_INTERNAL_1_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
PRIMER_LEFT_1=239,22
PRIMER_RIGHT_1=315,21
PRIMER_INTERNAL_1=270,24
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=59.168
PRIMER_INTERNAL_1_TM=60.121
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=10.00
PRIMER_PAIR_1_COMPL_END=8.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=4.193884
PRIMER_LEFT_2_PENALTY=0.072951
PRIMER_RIGHT_2_PENALTY=4.120932
PRIMER_INTERNAL_2_PENALTY=1.984975
PRIMER_LEFT_2_SEQUENCE=GACCCTCACTTCAGGGCATA
PRIMER_RIGHT_2_SEQUENCE=TCAATAGACTTCCTTTCTTCTGGG
PRIMER_INTERNAL_2_SEQUENCE=GATTTCTATGCATCCCATGCT
PRIMER_LEFT_2=210,20
PRIMER_RIGHT_2=293,24
PRIMER_INTERNAL_2=240,21
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=60.121
PRIMER_INTERNAL_2_TM=59.015
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_INTERNAL_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=84
PRIMER_PAIR_3_PENALTY=4.398830
PRIMER_LEFT_3_PENALTY=2.566538
PRIMER_RIGHT_3_PENALTY=1.832292
PRIMER_INTERNAL_3_PENALTY=4.120932
PRIMER_LEFT_3_SEQUENCE=CAAGATTTCTATGCATCCCATG
PRIMER_RIGHT_3_SEQUENCE=AAGCATGGGAGTGCAGATATC
PRIMER_INTERNAL_3_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
PRIMER_LEFT_3=237,22
PRIMER_RIGHT_3=317,21
PRIMER_INTERNAL_3=270,24
PRIMER_LEFT_3_TM=59.433
PRIMER_RIGHT_3_TM=59.168
PRIMER_INTERNAL_3_TM=60.121
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=5.5000
PRIMER_PAIR_3_COMPL_ANY=9.00
PRIMER_PAIR_3_COMPL_END=8.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=4.464586
PRIMER_LEFT_4_PENALTY=1.984975
PRIMER_RIGHT_4_PENALTY=2.479611
PRIMER_INTERNAL_4_PENALTY=4.120932
PRIMER_LEFT_4_SEQUENCE=GATTTCTATGCATCCCATGCT
PRIMER_RIGHT_4_SEQUENCE=GCATGGGAGTGCAGATATCTTT
PRIMER_INTERNAL_4_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA
PRIMER_LEFT_4=240,21
PRIMER_RIGHT_4=315,22
PRIMER_INTERNAL_4=270,24
PRIMER_LEFT_4_TM=59.015
PRIMER_RIGHT_4_TM=60.480
PRIMER_INTERNAL_4_TM=60.121
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=10.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH536
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCTTTTGACAGGGCTTCCAAAAAAAGGCAGNGCGGCCCGGGTGTCTCCCTGGCAGCGGCGAGAAGTCAGCGCCGGGGCTGGGGACTTGTAGGACAGAGTCGNAGNTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTG
SEQUENCE_INCLUDED_REGION=36,108
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH539
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGCATCTCTTTTTCACCTTCTGCATACTGGTGCTGAGCCTAAGGCCTTGAATGTAAGTGCTTTTTAAGTATTTACCAAGTGGAAGTTTCCATTTTATTTTCCACCTTATATTATAGTTGATTGTGTCCTAGTATTCCTCCTAAATTAGTGGCTGAGGAGAGAGAGTGTAGCAGCGATCTACTCCTCTGCTGGGACACACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC
SEQUENCE_INCLUDED_REGION=36,168
P3_COMMENT=CL is TCCTGCATCTCTTTTTCACC
P3_COMMENT=CR is CCACTAATTTAGGAGGAATACTAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.082643
PRIMER_LEFT_0_PENALTY=0.168025
PRIMER_RIGHT_0_PENALTY=2.914618
PRIMER_INTERNAL_0_PENALTY=2.328094
PRIMER_LEFT_0_SEQUENCE=TCTGCATACTGGTGCTGAGC
PRIMER_RIGHT_0_SEQUENCE=AAAATGGAAACTTCCACTTGG
PRIMER_INTERNAL_0_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
PRIMER_LEFT_0=58,20
PRIMER_RIGHT_0=134,21
PRIMER_INTERNAL_0=82,22
PRIMER_LEFT_0_TM=60.168
PRIMER_RIGHT_0_TM=58.085
PRIMER_INTERNAL_0_TM=59.672
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=10.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=3.253950
PRIMER_LEFT_1_PENALTY=0.168025
PRIMER_RIGHT_1_PENALTY=3.085925
PRIMER_INTERNAL_1_PENALTY=2.328094
PRIMER_LEFT_1_SEQUENCE=TCTGCATACTGGTGCTGAGC
PRIMER_RIGHT_1_SEQUENCE=AAAATGGAAACTTCCACTTGGT
PRIMER_INTERNAL_1_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
PRIMER_LEFT_1=58,20
PRIMER_RIGHT_1=134,22
PRIMER_INTERNAL_1=82,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=58.914
PRIMER_INTERNAL_1_TM=59.672
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=36.364
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=3.449505
PRIMER_LEFT_2_PENALTY=0.534888
PRIMER_RIGHT_2_PENALTY=2.914618
PRIMER_INTERNAL_2_PENALTY=2.328094
PRIMER_LEFT_2_SEQUENCE=CCTTCTGCATACTGGTGCTG
PRIMER_RIGHT_2_SEQUENCE=AAAATGGAAACTTCCACTTGG
PRIMER_INTERNAL_2_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
PRIMER_LEFT_2=55,20
PRIMER_RIGHT_2=134,21
PRIMER_INTERNAL_2=82,22
PRIMER_LEFT_2_TM=59.465
PRIMER_RIGHT_2_TM=58.085
PRIMER_INTERNAL_2_TM=59.672
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=10.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=3.596239
PRIMER_LEFT_3_PENALTY=0.681621
PRIMER_RIGHT_3_PENALTY=2.914618
PRIMER_INTERNAL_3_PENALTY=0.343950
PRIMER_LEFT_3_SEQUENCE=CACCTTCTGCATACTGGTGC
PRIMER_RIGHT_3_SEQUENCE=AAAATGGAAACTTCCACTTGG
PRIMER_INTERNAL_3_SEQUENCE=TGAGCCTAAGGCCTTGAATG
PRIMER_LEFT_3=53,20
PRIMER_RIGHT_3=134,21
PRIMER_INTERNAL_3=73,20
PRIMER_LEFT_3_TM=59.318
PRIMER_RIGHT_3_TM=58.085
PRIMER_INTERNAL_3_TM=60.344
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=10.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=3.602678
PRIMER_LEFT_4_PENALTY=0.168025
PRIMER_RIGHT_4_PENALTY=3.434653
PRIMER_INTERNAL_4_PENALTY=2.328094
PRIMER_LEFT_4_SEQUENCE=TCTGCATACTGGTGCTGAGC
PRIMER_RIGHT_4_SEQUENCE=AAATGGAAACTTCCACTTGGT
PRIMER_INTERNAL_4_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT
PRIMER_LEFT_4=58,20
PRIMER_RIGHT_4=133,21
PRIMER_INTERNAL_4=82,22
PRIMER_LEFT_4_TM=60.168
PRIMER_RIGHT_4_TM=57.565
PRIMER_INTERNAL_4_TM=59.672
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=10.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH554
SEQUENCE_TEMPLATE=GCTCCACTGGGCCTAGGTATGCCATAGAGGCCACCCCTCTGAAGGGCATAGGTGGTAAATCTTGGTGGCATCCACAGGGTGCCAATGCTGCAGGCATCCAGAGTGTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
SEQUENCE_INCLUDED_REGION=0,110
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.070177
PRIMER_LEFT_0_PENALTY=0.061961
PRIMER_RIGHT_0_PENALTY=0.008216
PRIMER_INTERNAL_0_PENALTY=0.751463
PRIMER_LEFT_0_SEQUENCE=TGGGCCTAGGTATGCCATAG
PRIMER_RIGHT_0_SEQUENCE=TACACTCTGGATGCCTGCAG
PRIMER_INTERNAL_0_SEQUENCE=GGTAAATCTTGGTGGCATCC
PRIMER_LEFT_0=7,20
PRIMER_RIGHT_0=106,20
PRIMER_INTERNAL_0=53,20
PRIMER_LEFT_0_TM=59.938
PRIMER_RIGHT_0_TM=60.008
PRIMER_INTERNAL_0_TM=59.249
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.974053
PRIMER_LEFT_1_PENALTY=0.965837
PRIMER_RIGHT_1_PENALTY=0.008216
PRIMER_INTERNAL_1_PENALTY=0.751463
PRIMER_LEFT_1_SEQUENCE=AGGTATGCCATAGAGGCCAC
PRIMER_RIGHT_1_SEQUENCE=TACACTCTGGATGCCTGCAG
PRIMER_INTERNAL_1_SEQUENCE=GGTAAATCTTGGTGGCATCC
PRIMER_LEFT_1=14,20
PRIMER_RIGHT_1=106,20
PRIMER_INTERNAL_1=53,20
PRIMER_LEFT_1_TM=59.034
PRIMER_RIGHT_1_TM=60.008
PRIMER_INTERNAL_1_TM=59.249
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=0.974053
PRIMER_LEFT_2_PENALTY=0.965837
PRIMER_RIGHT_2_PENALTY=0.008216
PRIMER_INTERNAL_2_PENALTY=0.751463
PRIMER_LEFT_2_SEQUENCE=AGGTATGCCATAGAGGCCAC
PRIMER_RIGHT_2_SEQUENCE=CTACACTCTGGATGCCTGCA
PRIMER_INTERNAL_2_SEQUENCE=GGTAAATCTTGGTGGCATCC
PRIMER_LEFT_2=14,20
PRIMER_RIGHT_2=107,20
PRIMER_INTERNAL_2=53,20
PRIMER_LEFT_2_TM=59.034
PRIMER_RIGHT_2_TM=60.008
PRIMER_INTERNAL_2_TM=59.249
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=0.985788
PRIMER_LEFT_3_PENALTY=0.977572
PRIMER_RIGHT_3_PENALTY=0.008216
PRIMER_INTERNAL_3_PENALTY=0.751463
PRIMER_LEFT_3_SEQUENCE=GGGCCTAGGTATGCCATAGA
PRIMER_RIGHT_3_SEQUENCE=TACACTCTGGATGCCTGCAG
PRIMER_INTERNAL_3_SEQUENCE=GGTAAATCTTGGTGGCATCC
PRIMER_LEFT_3=8,20
PRIMER_RIGHT_3=106,20
PRIMER_INTERNAL_3=53,20
PRIMER_LEFT_3_TM=59.022
PRIMER_RIGHT_3_TM=60.008
PRIMER_INTERNAL_3_TM=59.249
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=0.985788
PRIMER_LEFT_4_PENALTY=0.977572
PRIMER_RIGHT_4_PENALTY=0.008216
PRIMER_INTERNAL_4_PENALTY=0.751463
PRIMER_LEFT_4_SEQUENCE=GGGCCTAGGTATGCCATAGA
PRIMER_RIGHT_4_SEQUENCE=CTACACTCTGGATGCCTGCA
PRIMER_INTERNAL_4_SEQUENCE=GGTAAATCTTGGTGGCATCC
PRIMER_LEFT_4=8,20
PRIMER_RIGHT_4=107,20
PRIMER_INTERNAL_4=53,20
PRIMER_LEFT_4_TM=59.022
PRIMER_RIGHT_4_TM=60.008
PRIMER_INTERNAL_4_TM=59.249
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH556
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAATCATTAATAAAATTAAGATGTTAAAACTGAATATCCATCTCACCAATATTTTTTGAGCACCTTCCATATTCCAAGCACTATTCAAGATAGTGGTGGTCAATGAACAGGAAACACAATGTTCTTGTACTTGAGGAGTTTATGTGATGGTTAGAGAGACAGACAATAAACAATAAACAAATAGCACTTCTCATCATGGTGGACCCTAGAGATAAGATATTTCAGGACTACAGCAAGAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC
SEQUENCE_INCLUDED_REGION=36,205
P3_COMMENT=CL is GTTAAAACTGAATATCCATCTCACC
P3_COMMENT=CR is CCACCATGATGAGAAGTGCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.420012
PRIMER_LEFT_0_PENALTY=4.355025
PRIMER_RIGHT_0_PENALTY=0.064987
PRIMER_INTERNAL_0_PENALTY=4.129072
PRIMER_LEFT_0_SEQUENCE=CATCTCACCAATATTTTTTGAGCA
PRIMER_RIGHT_0_SEQUENCE=TTCCTGTTCATTGACCACCA
PRIMER_INTERNAL_0_SEQUENCE=TTCCATATTCCAAGCACTATTCAA
PRIMER_LEFT_0=76,24
PRIMER_RIGHT_0=150,20
PRIMER_INTERNAL_0=102,24
PRIMER_LEFT_0_TM=60.355
PRIMER_RIGHT_0_TM=59.935
PRIMER_INTERNAL_0_TM=59.871
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=4.580906
PRIMER_LEFT_1_PENALTY=4.515919
PRIMER_RIGHT_1_PENALTY=0.064987
PRIMER_INTERNAL_1_PENALTY=0.466916
PRIMER_LEFT_1_SEQUENCE=TCCATCTCACCAATATTTTTTGA
PRIMER_RIGHT_1_SEQUENCE=TTCCTGTTCATTGACCACCA
PRIMER_INTERNAL_1_SEQUENCE=GCACCTTCCATATTCCAAGC
PRIMER_LEFT_1=74,23
PRIMER_RIGHT_1=150,20
PRIMER_INTERNAL_1=97,20
PRIMER_LEFT_1_TM=58.484
PRIMER_RIGHT_1_TM=59.935
PRIMER_INTERNAL_1_TM=59.533
PRIMER_LEFT_1_GC_PERCENT=30.435
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=4.670331
PRIMER_LEFT_2_PENALTY=4.605343
PRIMER_RIGHT_2_PENALTY=0.064987
PRIMER_INTERNAL_2_PENALTY=1.427808
PRIMER_LEFT_2_SEQUENCE=TCCATCTCACCAATATTTTTTGAG
PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTCATTGACCACCA
PRIMER_INTERNAL_2_SEQUENCE=CACCTTCCATATTCCAAGCA
PRIMER_LEFT_2=74,24
PRIMER_RIGHT_2=150,20
PRIMER_INTERNAL_2=98,20
PRIMER_LEFT_2_TM=59.395
PRIMER_RIGHT_2_TM=59.935
PRIMER_INTERNAL_2_TM=58.572
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=4.699503
PRIMER_LEFT_3_PENALTY=4.336505
PRIMER_RIGHT_3_PENALTY=0.362999
PRIMER_INTERNAL_3_PENALTY=5.088471
PRIMER_LEFT_3_SEQUENCE=GAACAGGAAACACAATGTTCTTG
PRIMER_RIGHT_3_SEQUENCE=TCCACCATGATGAGAAGTGC
PRIMER_INTERNAL_3_SEQUENCE=TGTGATGGTTAGAGAGACAGACAA
PRIMER_LEFT_3=142,23
PRIMER_RIGHT_3=240,20
PRIMER_INTERNAL_3=180,24
PRIMER_LEFT_3_TM=58.663
PRIMER_RIGHT_3_TM=59.637
PRIMER_INTERNAL_3_TM=58.912
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=4.699503
PRIMER_LEFT_4_PENALTY=4.336505
PRIMER_RIGHT_4_PENALTY=0.362999
PRIMER_INTERNAL_4_PENALTY=5.088471
PRIMER_LEFT_4_SEQUENCE=TGAACAGGAAACACAATGTTCTT
PRIMER_RIGHT_4_SEQUENCE=TCCACCATGATGAGAAGTGC
PRIMER_INTERNAL_4_SEQUENCE=TGTGATGGTTAGAGAGACAGACAA
PRIMER_LEFT_4=141,23
PRIMER_RIGHT_4=240,20
PRIMER_INTERNAL_4=180,24
PRIMER_LEFT_4_TM=58.663
PRIMER_RIGHT_4_TM=59.637
PRIMER_INTERNAL_4_TM=58.912
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH560
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACGTTCTCCGTCCTATTTTAAGAACTCAGTTCTTCATAAGACTTGTGTGGTTTTCGATTTTTTCCCAAGTCTGGTTGATCCTTGTGTNGTNATTTTTTTAAATGTGTATCGTCTGTTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
SEQUENCE_INCLUDED_REGION=36,121
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.226148
PRIMER_LEFT_0_PENALTY=4.129255
PRIMER_RIGHT_0_PENALTY=0.096893
PRIMER_INTERNAL_0_PENALTY=3.813135
PRIMER_LEFT_0_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAA
PRIMER_RIGHT_0_SEQUENCE=GGATCAACCAGACTTGGGAA
PRIMER_INTERNAL_0_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
PRIMER_LEFT_0=39,23
PRIMER_RIGHT_0=118,20
PRIMER_INTERNAL_0=72,23
PRIMER_LEFT_0_TM=58.871
PRIMER_RIGHT_0_TM=59.903
PRIMER_INTERNAL_0_TM=59.187
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=4.491833
PRIMER_LEFT_1_PENALTY=4.394940
PRIMER_RIGHT_1_PENALTY=0.096893
PRIMER_INTERNAL_1_PENALTY=3.813135
PRIMER_LEFT_1_SEQUENCE=CGTTCTCCGTCCTATTTTAAGA
PRIMER_RIGHT_1_SEQUENCE=GGATCAACCAGACTTGGGAA
PRIMER_INTERNAL_1_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=118,20
PRIMER_INTERNAL_1=72,23
PRIMER_LEFT_1_TM=57.605
PRIMER_RIGHT_1_TM=59.903
PRIMER_INTERNAL_1_TM=59.187
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=4.498797
PRIMER_LEFT_2_PENALTY=4.401904
PRIMER_RIGHT_2_PENALTY=0.096893
PRIMER_INTERNAL_2_PENALTY=3.813135
PRIMER_LEFT_2_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAAC
PRIMER_RIGHT_2_SEQUENCE=GGATCAACCAGACTTGGGAA
PRIMER_INTERNAL_2_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
PRIMER_LEFT_2=39,24
PRIMER_RIGHT_2=118,20
PRIMER_INTERNAL_2=72,23
PRIMER_LEFT_2_TM=59.598
PRIMER_RIGHT_2_TM=59.903
PRIMER_INTERNAL_2_TM=59.187
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=4.498797
PRIMER_LEFT_3_PENALTY=4.401904
PRIMER_RIGHT_3_PENALTY=0.096893
PRIMER_INTERNAL_3_PENALTY=3.813135
PRIMER_LEFT_3_SEQUENCE=ACGTTCTCCGTCCTATTTTAAGAA
PRIMER_RIGHT_3_SEQUENCE=GGATCAACCAGACTTGGGAA
PRIMER_INTERNAL_3_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA
PRIMER_LEFT_3=38,24
PRIMER_RIGHT_3=118,20
PRIMER_INTERNAL_3=72,23
PRIMER_LEFT_3_TM=59.598
PRIMER_RIGHT_3_TM=59.903
PRIMER_INTERNAL_3_TM=59.187
PRIMER_LEFT_3_GC_PERCENT=37.500
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=4.624066
PRIMER_LEFT_4_PENALTY=4.129255
PRIMER_RIGHT_4_PENALTY=0.494811
PRIMER_INTERNAL_4_PENALTY=3.554504
PRIMER_LEFT_4_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAA
PRIMER_RIGHT_4_SEQUENCE=AGGATCAACCAGACTTGGGA
PRIMER_INTERNAL_4_SEQUENCE=GACTTGTGTGGTTTTCGATTTTT
PRIMER_LEFT_4=39,23
PRIMER_RIGHT_4=119,20
PRIMER_INTERNAL_4=77,23
PRIMER_LEFT_4_TM=58.871
PRIMER_RIGHT_4_TM=59.505
PRIMER_INTERNAL_4_TM=59.445
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH563
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGCGAGAAAGGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCG
SEQUENCE_INCLUDED_REGION=36,14
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH568
SEQUENCE_TEMPLATE=TCTGGGGATGCCTAGAACAAAAACAGACACCCTCGGCTTCTTCTCACCAGTCCACATGGGTGCCAAACAATCCCACATTCCTACATCCTCCCCACTGGGCTGCCTCCTTCACAACCTCACCAAACTTGGCTTACCGGGAAGCATAAAGCCAAAGCATTTAGTCTTTTATTGCAACATGGTCTGGCTGCAATACACATTAGATAATGACAGCCGNTGGTTGGAAAATGTCACCTNTNNNTTGNAANTT
SEQUENCE_INCLUDED_REGION=0,247
P3_COMMENT=CL is AACAAAAACAGACACCCTCG
P3_COMMENT=CR is GTATTGCAGCCAGACCATGT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.085545
PRIMER_LEFT_0_PENALTY=0.005232
PRIMER_RIGHT_0_PENALTY=0.080313
PRIMER_INTERNAL_0_PENALTY=0.902727
PRIMER_LEFT_0_SEQUENCE=ACAAAAACAGACACCCTCGG
PRIMER_RIGHT_0_SEQUENCE=CAGTGGGGAGGATGTAGGAA
PRIMER_INTERNAL_0_SEQUENCE=CTTCTCACCAGTCCACATGG
PRIMER_LEFT_0=16,20
PRIMER_RIGHT_0=96,20
PRIMER_INTERNAL_0=39,20
PRIMER_LEFT_0_TM=60.005
PRIMER_RIGHT_0_TM=59.920
PRIMER_INTERNAL_0_TM=59.097
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=81
PRIMER_PAIR_1_PENALTY=0.146636
PRIMER_LEFT_1_PENALTY=0.066323
PRIMER_RIGHT_1_PENALTY=0.080313
PRIMER_INTERNAL_1_PENALTY=0.385756
PRIMER_LEFT_1_SEQUENCE=CTGGGGATGCCTAGAACAAA
PRIMER_RIGHT_1_SEQUENCE=CAGTGGGGAGGATGTAGGAA
PRIMER_INTERNAL_1_SEQUENCE=GGCTTCTTCTCACCAGTCCA
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=96,20
PRIMER_INTERNAL_1=34,20
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=59.920
PRIMER_INTERNAL_1_TM=60.386
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=0.398226
PRIMER_LEFT_2_PENALTY=0.385756
PRIMER_RIGHT_2_PENALTY=0.012470
PRIMER_INTERNAL_2_PENALTY=0.080313
PRIMER_LEFT_2_SEQUENCE=GGCTTCTTCTCACCAGTCCA
PRIMER_RIGHT_2_SEQUENCE=GCCAAGTTTGGTGAGGTTGT
PRIMER_INTERNAL_2_SEQUENCE=TTCCTACATCCTCCCCACTG
PRIMER_LEFT_2=34,20
PRIMER_RIGHT_2=129,20
PRIMER_INTERNAL_2=77,20
PRIMER_LEFT_2_TM=60.386
PRIMER_RIGHT_2_TM=60.012
PRIMER_INTERNAL_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.414512
PRIMER_LEFT_3_PENALTY=0.402042
PRIMER_RIGHT_3_PENALTY=0.012470
PRIMER_INTERNAL_3_PENALTY=0.080313
PRIMER_LEFT_3_SEQUENCE=CTCGGCTTCTTCTCACCAGT
PRIMER_RIGHT_3_SEQUENCE=GCCAAGTTTGGTGAGGTTGT
PRIMER_INTERNAL_3_SEQUENCE=TTCCTACATCCTCCCCACTG
PRIMER_LEFT_3=31,20
PRIMER_RIGHT_3=129,20
PRIMER_INTERNAL_3=77,20
PRIMER_LEFT_3_TM=59.598
PRIMER_RIGHT_3_TM=60.012
PRIMER_INTERNAL_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=0.419393
PRIMER_LEFT_4_PENALTY=0.066323
PRIMER_RIGHT_4_PENALTY=0.353069
PRIMER_INTERNAL_4_PENALTY=0.385756
PRIMER_LEFT_4_SEQUENCE=CTGGGGATGCCTAGAACAAA
PRIMER_RIGHT_4_SEQUENCE=AGGAATGTGGGATTGTTTGG
PRIMER_INTERNAL_4_SEQUENCE=GGCTTCTTCTCACCAGTCCA
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=81,20
PRIMER_INTERNAL_4=34,20
PRIMER_LEFT_4_TM=60.066
PRIMER_RIGHT_4_TM=59.647
PRIMER_INTERNAL_4_TM=60.386
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH569
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGAGATAAGACCTTGAAATAGTAGGATTCTTACAGGTGTATTACATGGTTTGAATTGAGATGATTACATGGGCATGTCTCACCTATAGTCAGGTAAACAAGTCTGGAAATCAAGAAATGGAAATGGTTTCTCTGTTGAACATAATAACCCCTTTGTAGGCTTTTTTAACTTTTTGTCCTGGTAACTATCTTGATTTGGTGCTCTTAGTTTCCAGTAACGGGAATTATTTCATTGGGGTTCCAAGAGGGTTGCATTAAACTTTATGAGACTGCCACTTGACCATTTGAGGATCCTTACGTTACTAAACAAACAGGCAAAAAGGGACTATTCTACTGGCTA
SEQUENCE_INCLUDED_REGION=36,342
P3_COMMENT=CL is CATGGGCATGTCTCACCTAT
P3_COMMENT=CR is TTTGCCTGTTTGTTTAGTAACG
P3_COMMENT=CM is GTCAGGTAAACAAGTCTGGAAATC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.510318
PRIMER_LEFT_0_PENALTY=0.096855
PRIMER_RIGHT_0_PENALTY=0.413463
PRIMER_INTERNAL_0_PENALTY=0.711868
PRIMER_LEFT_0_SEQUENCE=TTTCATTGGGGTTCCAAGAG
PRIMER_RIGHT_0_SEQUENCE=TCCCTTTTTGCCTGTTTGTT
PRIMER_INTERNAL_0_SEQUENCE=CTGCCACTTGACCATTTGAG
PRIMER_LEFT_0=265,20
PRIMER_RIGHT_0=362,20
PRIMER_INTERNAL_0=307,20
PRIMER_LEFT_0_TM=59.903
PRIMER_RIGHT_0_TM=59.587
PRIMER_INTERNAL_0_TM=59.288
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=0.882263
PRIMER_LEFT_1_PENALTY=0.096855
PRIMER_RIGHT_1_PENALTY=0.785407
PRIMER_INTERNAL_1_PENALTY=0.711868
PRIMER_LEFT_1_SEQUENCE=TTTCATTGGGGTTCCAAGAG
PRIMER_RIGHT_1_SEQUENCE=AGTCCCTTTTTGCCTGTTTG
PRIMER_INTERNAL_1_SEQUENCE=CTGCCACTTGACCATTTGAG
PRIMER_LEFT_1=265,20
PRIMER_RIGHT_1=364,20
PRIMER_INTERNAL_1=307,20
PRIMER_LEFT_1_TM=59.903
PRIMER_RIGHT_1_TM=59.215
PRIMER_INTERNAL_1_TM=59.288
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=0.989299
PRIMER_LEFT_2_PENALTY=0.096855
PRIMER_RIGHT_2_PENALTY=0.892444
PRIMER_INTERNAL_2_PENALTY=0.711868
PRIMER_LEFT_2_SEQUENCE=TTTCATTGGGGTTCCAAGAG
PRIMER_RIGHT_2_SEQUENCE=CCCTTTTTGCCTGTTTGTTT
PRIMER_INTERNAL_2_SEQUENCE=CTGCCACTTGACCATTTGAG
PRIMER_LEFT_2=265,20
PRIMER_RIGHT_2=361,20
PRIMER_INTERNAL_2=307,20
PRIMER_LEFT_2_TM=59.903
PRIMER_RIGHT_2_TM=59.108
PRIMER_INTERNAL_2_TM=59.288
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=1.020213
PRIMER_LEFT_3_PENALTY=0.096855
PRIMER_RIGHT_3_PENALTY=0.923358
PRIMER_INTERNAL_3_PENALTY=0.711868
PRIMER_LEFT_3_SEQUENCE=TTTCATTGGGGTTCCAAGAG
PRIMER_RIGHT_3_SEQUENCE=GTCCCTTTTTGCCTGTTTGT
PRIMER_INTERNAL_3_SEQUENCE=CTGCCACTTGACCATTTGAG
PRIMER_LEFT_3=265,20
PRIMER_RIGHT_3=363,20
PRIMER_INTERNAL_3=307,20
PRIMER_LEFT_3_TM=59.903
PRIMER_RIGHT_3_TM=59.077
PRIMER_INTERNAL_3_TM=59.288
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=1.122862
PRIMER_LEFT_4_PENALTY=0.096855
PRIMER_RIGHT_4_PENALTY=1.026007
PRIMER_INTERNAL_4_PENALTY=0.711868
PRIMER_LEFT_4_SEQUENCE=TTTCATTGGGGTTCCAAGAG
PRIMER_RIGHT_4_SEQUENCE=AGTCCCTTTTTGCCTGTTTGT
PRIMER_INTERNAL_4_SEQUENCE=CTGCCACTTGACCATTTGAG
PRIMER_LEFT_4=265,20
PRIMER_RIGHT_4=364,21
PRIMER_INTERNAL_4=307,20
PRIMER_LEFT_4_TM=59.903
PRIMER_RIGHT_4_TM=60.026
PRIMER_INTERNAL_4_TM=59.288
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH570
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGATAACGTTGGGAGTNGTAAAAACTCAGTTTATAATCACATTGAATGACATACACTTTAAGGCCCTGAAGTATGTTTCTGTTTTGTCTCTTTTTAAATTCTTATACAGTGGGTATGTGACAGGAATAGAATCAAATGAAGAGGAAATTACAGGGCAGCAACTTTGGACAATAGTGAAAAGGAAAGATGCATTGCTTGCTTTAGTGTTCATGTGACCACTTTATCCTAGAAGCAGAAATTAAGTGGCAGAGAGTCACCTGTGAGTGTGACTAATGCCGTCAATTCCAGGAGAACAAGAAAAAAAAAGCCTTCTGTCCCAACT
SEQUENCE_INCLUDED_REGION=36,326
P3_COMMENT=CL is GGCCCTGAAGTATGTTTCTG
P3_COMMENT=CR is GTTGGGACAGAAGGCTTTTT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.919073
PRIMER_LEFT_0_PENALTY=0.360621
PRIMER_RIGHT_0_PENALTY=0.558452
PRIMER_INTERNAL_0_PENALTY=4.182061
PRIMER_LEFT_0_SEQUENCE=GGAAAGATGCATTGCTTGCT
PRIMER_RIGHT_0_SEQUENCE=CACAGGTGACTCTCTGCCAC
PRIMER_INTERNAL_0_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
PRIMER_LEFT_0=220,20
PRIMER_RIGHT_0=301,20
PRIMER_INTERNAL_0=250,24
PRIMER_LEFT_0_TM=60.361
PRIMER_RIGHT_0_TM=59.442
PRIMER_INTERNAL_0_TM=60.182
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=0.919073
PRIMER_LEFT_1_PENALTY=0.360621
PRIMER_RIGHT_1_PENALTY=0.558452
PRIMER_INTERNAL_1_PENALTY=4.182061
PRIMER_LEFT_1_SEQUENCE=AGGAAAGATGCATTGCTTGC
PRIMER_RIGHT_1_SEQUENCE=CACAGGTGACTCTCTGCCAC
PRIMER_INTERNAL_1_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
PRIMER_LEFT_1=219,20
PRIMER_RIGHT_1=301,20
PRIMER_INTERNAL_1=250,24
PRIMER_LEFT_1_TM=60.361
PRIMER_RIGHT_1_TM=59.442
PRIMER_INTERNAL_1_TM=60.182
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.974220
PRIMER_LEFT_2_PENALTY=0.360621
PRIMER_RIGHT_2_PENALTY=0.613599
PRIMER_INTERNAL_2_PENALTY=4.182061
PRIMER_LEFT_2_SEQUENCE=GGAAAGATGCATTGCTTGCT
PRIMER_RIGHT_2_SEQUENCE=TCACAGGTGACTCTCTGCCA
PRIMER_INTERNAL_2_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
PRIMER_LEFT_2=220,20
PRIMER_RIGHT_2=302,20
PRIMER_INTERNAL_2=250,24
PRIMER_LEFT_2_TM=60.361
PRIMER_RIGHT_2_TM=60.614
PRIMER_INTERNAL_2_TM=60.182
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.974220
PRIMER_LEFT_3_PENALTY=0.360621
PRIMER_RIGHT_3_PENALTY=0.613599
PRIMER_INTERNAL_3_PENALTY=4.182061
PRIMER_LEFT_3_SEQUENCE=AGGAAAGATGCATTGCTTGC
PRIMER_RIGHT_3_SEQUENCE=TCACAGGTGACTCTCTGCCA
PRIMER_INTERNAL_3_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA
PRIMER_LEFT_3=219,20
PRIMER_RIGHT_3=302,20
PRIMER_INTERNAL_3=250,24
PRIMER_LEFT_3_TM=60.361
PRIMER_RIGHT_3_TM=60.614
PRIMER_INTERNAL_3_TM=60.182
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=1.653872
PRIMER_LEFT_4_PENALTY=0.558452
PRIMER_RIGHT_4_PENALTY=1.095420
PRIMER_INTERNAL_4_PENALTY=0.460175
PRIMER_LEFT_4_SEQUENCE=GTGGCAGAGAGTCACCTGTG
PRIMER_RIGHT_4_SEQUENCE=TGGGACAGAAGGCTTTTTTTT
PRIMER_INTERNAL_4_SEQUENCE=CTAATGCCGTCAATTCCAGG
PRIMER_LEFT_4=282,20
PRIMER_RIGHT_4=358,21
PRIMER_INTERNAL_4=309,20
PRIMER_LEFT_4_TM=59.442
PRIMER_RIGHT_4_TM=60.095
PRIMER_INTERNAL_4_TM=60.460
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH572
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAAGGCTGAATCTTAACAGCCTTTTGATGCCAAAGCCACTTTCAGTCTTAATTCTTTTTGGAGCCTAAGATCAGTGCAACCCTCCAAGGCTCCCCAGTATCTGGCACATCTTTCCCTTTTCATCTCCGTTTGTGTGTTTGGCCAAATAATATCTCCCCCAGGGACGTCCTCTTTCTAATCCCTGAAACCTGAGAAAATGTTATCTTATGCAGTGCTATGGTTTGAATGTGTCCCCCACAAAGCACACATTAGAAACTTAATCCCCAGTGCAACAGTNTTGGGAGGTGGGGCCTAATGGAAGATGTTTAGGTCATAAAAGGTACCGAGCTCGAATTCGTAATC
SEQUENCE_INCLUDED_REGION=36,283
P3_COMMENT=CL is AATCTTAACAGCCTTTTGATGC
P3_COMMENT=CR is TGTTGCACTGGGGATTAAGT
P3_COMMENT=CM is AAAGCCACTTTCAGTCTTAATTCT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.298763
PRIMER_LEFT_0_PENALTY=0.248900
PRIMER_RIGHT_0_PENALTY=0.049863
PRIMER_INTERNAL_0_PENALTY=0.300243
PRIMER_LEFT_0_SEQUENCE=CTTTTGATGCCAAAGCCACT
PRIMER_RIGHT_0_SEQUENCE=ATGTGCCAGATACTGGGGAG
PRIMER_INTERNAL_0_SEQUENCE=GCCTAAGATCAGTGCAACCC
PRIMER_LEFT_0=58,20
PRIMER_RIGHT_0=146,20
PRIMER_INTERNAL_0=100,20
PRIMER_LEFT_0_TM=60.249
PRIMER_RIGHT_0_TM=59.950
PRIMER_INTERNAL_0_TM=59.700
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=0.298763
PRIMER_LEFT_1_PENALTY=0.248900
PRIMER_RIGHT_1_PENALTY=0.049863
PRIMER_INTERNAL_1_PENALTY=0.300243
PRIMER_LEFT_1_SEQUENCE=CTTTTGATGCCAAAGCCACT
PRIMER_RIGHT_1_SEQUENCE=AGATGTGCCAGATACTGGGG
PRIMER_INTERNAL_1_SEQUENCE=GCCTAAGATCAGTGCAACCC
PRIMER_LEFT_1=58,20
PRIMER_RIGHT_1=148,20
PRIMER_INTERNAL_1=100,20
PRIMER_LEFT_1_TM=60.249
PRIMER_RIGHT_1_TM=59.950
PRIMER_INTERNAL_1_TM=59.700
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.298763
PRIMER_LEFT_2_PENALTY=0.248900
PRIMER_RIGHT_2_PENALTY=0.049863
PRIMER_INTERNAL_2_PENALTY=0.300243
PRIMER_LEFT_2_SEQUENCE=ACAGCCTTTTGATGCCAAAG
PRIMER_RIGHT_2_SEQUENCE=ATGTGCCAGATACTGGGGAG
PRIMER_INTERNAL_2_SEQUENCE=GCCTAAGATCAGTGCAACCC
PRIMER_LEFT_2=53,20
PRIMER_RIGHT_2=146,20
PRIMER_INTERNAL_2=100,20
PRIMER_LEFT_2_TM=60.249
PRIMER_RIGHT_2_TM=59.950
PRIMER_INTERNAL_2_TM=59.700
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=0.298763
PRIMER_LEFT_3_PENALTY=0.248900
PRIMER_RIGHT_3_PENALTY=0.049863
PRIMER_INTERNAL_3_PENALTY=0.300243
PRIMER_LEFT_3_SEQUENCE=ACAGCCTTTTGATGCCAAAG
PRIMER_RIGHT_3_SEQUENCE=AGATGTGCCAGATACTGGGG
PRIMER_INTERNAL_3_SEQUENCE=GCCTAAGATCAGTGCAACCC
PRIMER_LEFT_3=53,20
PRIMER_RIGHT_3=148,20
PRIMER_INTERNAL_3=100,20
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=59.950
PRIMER_INTERNAL_3_TM=59.700
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=0.309566
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.049863
PRIMER_INTERNAL_4_PENALTY=0.300243
PRIMER_LEFT_4_SEQUENCE=ATGCCAAAGCCACTTTCAGT
PRIMER_RIGHT_4_SEQUENCE=ATGTGCCAGATACTGGGGAG
PRIMER_INTERNAL_4_SEQUENCE=GCCTAAGATCAGTGCAACCC
PRIMER_LEFT_4=64,20
PRIMER_RIGHT_4=146,20
PRIMER_INTERNAL_4=100,20
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=59.950
PRIMER_INTERNAL_4_TM=59.700
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=83
=
SEQUENCE_ID=MH574
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTAACCATTATTAGATTTTCTCCACTCAAGGCATCTCCTCATAAGCAGTNTTTCCTGACCATAAATCTTAGTTGTCCTATCCTCGGACACTTGTTATTTCATCACCCTGCCTTTGCAATTATCTGAAAATATCTTTGGTTATACAATTTATCGTGTCTCCCCACCCAACCCCCATCTCCTAGAACCCACTTCCCTAACCCGAAAATACGGGGCAAGAAACCTGTCTGTACTGGTCACAACCCTATCCCTGGGCCTACAACCGTGTCTGAGTCATAGTAATCCCTCAACAAATTTTGTGTGAACCGAAAGAAAAAAACACTTAAGACAATCTAAG
SEQUENCE_INCLUDED_REGION=36,340
P3_COMMENT=CL is TTCTCCACTCAAGGCATCTC
P3_COMMENT=CR is ACTCAGACACGGTTGTAGGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.416477
PRIMER_LEFT_0_PENALTY=0.349041
PRIMER_RIGHT_0_PENALTY=0.067436
PRIMER_INTERNAL_0_PENALTY=1.118579
PRIMER_LEFT_0_SEQUENCE=TCCACTCAAGGCATCTCCTC
PRIMER_RIGHT_0_SEQUENCE=CAAAGGCAGGGTGATGAAAT
PRIMER_INTERNAL_0_SEQUENCE=TGTCCTATCCTCGGACACTTG
PRIMER_LEFT_0=62,20
PRIMER_RIGHT_0=156,20
PRIMER_INTERNAL_0=114,21
PRIMER_LEFT_0_TM=60.349
PRIMER_RIGHT_0_TM=59.933
PRIMER_INTERNAL_0_TM=60.119
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=0.416477
PRIMER_LEFT_1_PENALTY=0.349041
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_INTERNAL_1_PENALTY=1.118579
PRIMER_LEFT_1_SEQUENCE=TCTCCACTCAAGGCATCTCC
PRIMER_RIGHT_1_SEQUENCE=CAAAGGCAGGGTGATGAAAT
PRIMER_INTERNAL_1_SEQUENCE=TGTCCTATCCTCGGACACTTG
PRIMER_LEFT_1=60,20
PRIMER_RIGHT_1=156,20
PRIMER_INTERNAL_1=114,21
PRIMER_LEFT_1_TM=60.349
PRIMER_RIGHT_1_TM=59.933
PRIMER_INTERNAL_1_TM=60.119
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=0.662644
PRIMER_LEFT_2_PENALTY=0.595208
PRIMER_RIGHT_2_PENALTY=0.067436
PRIMER_INTERNAL_2_PENALTY=1.118579
PRIMER_LEFT_2_SEQUENCE=CTCCACTCAAGGCATCTCCT
PRIMER_RIGHT_2_SEQUENCE=CAAAGGCAGGGTGATGAAAT
PRIMER_INTERNAL_2_SEQUENCE=TGTCCTATCCTCGGACACTTG
PRIMER_LEFT_2=61,20
PRIMER_RIGHT_2=156,20
PRIMER_INTERNAL_2=114,21
PRIMER_LEFT_2_TM=59.405
PRIMER_RIGHT_2_TM=59.933
PRIMER_INTERNAL_2_TM=60.119
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.740789
PRIMER_LEFT_3_PENALTY=0.040146
PRIMER_RIGHT_3_PENALTY=0.700643
PRIMER_INTERNAL_3_PENALTY=1.067353
PRIMER_LEFT_3_SEQUENCE=CACCCTGCCTTTGCAATTAT
PRIMER_RIGHT_3_SEQUENCE=GGGTTAGGGAAGTGGGTTCT
PRIMER_INTERNAL_3_SEQUENCE=AATTTATCGTGTCTCCCCACC
PRIMER_LEFT_3=144,20
PRIMER_RIGHT_3=241,20
PRIMER_INTERNAL_3=186,21
PRIMER_LEFT_3_TM=59.960
PRIMER_RIGHT_3_TM=59.299
PRIMER_INTERNAL_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=0.744507
PRIMER_LEFT_4_PENALTY=0.040146
PRIMER_RIGHT_4_PENALTY=0.704360
PRIMER_INTERNAL_4_PENALTY=1.067353
PRIMER_LEFT_4_SEQUENCE=CACCCTGCCTTTGCAATTAT
PRIMER_RIGHT_4_SEQUENCE=GTGGGTTCTAGGAGATGGGG
PRIMER_INTERNAL_4_SEQUENCE=AATTTATCGTGTCTCCCCACC
PRIMER_LEFT_4=144,20
PRIMER_RIGHT_4=230,20
PRIMER_INTERNAL_4=186,21
PRIMER_LEFT_4_TM=59.960
PRIMER_RIGHT_4_TM=60.704
PRIMER_INTERNAL_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH576
SEQUENCE_TEMPLATE=GCTATATAGACTACAATATGCCTCTTAAATTTATCCCAATTCACTTAAAGTTTATCCTTCCCTGTACTGCAGCCTAAAAACTCCAGGCAGTAATCTGGACAAACGTAGGGCTCAATTCTTTGTTTCTATCTTTTAGGCATCATTGTCCCAGTCTAAATGATGTCTATATTTTCTTTGCATCTTAGTTATTTCAGGTGAGAGATTAAATCATGTCTTTGTTATTCCATCTTGGTTGGAATTGGAAGTCCTAGGTTANGTGTAAATTTAAGAAATGAAAATGATATGCGTGGGCAATTAAGTTGTCAG
SEQUENCE_INCLUDED_REGION=0,306
P3_COMMENT=CL is CAATTCACTTAAAGTTTATCCTTCC
P3_COMMENT=CR is CTTAATTGCCCACGCATATC
P3_COMMENT=CM is TGTACTGCAGCCTAAAAACTCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.842445
PRIMER_LEFT_0_PENALTY=0.377925
PRIMER_RIGHT_0_PENALTY=0.464520
PRIMER_INTERNAL_0_PENALTY=0.254613
PRIMER_LEFT_0_SEQUENCE=CAGCCTAAAAACTCCAGGCA
PRIMER_RIGHT_0_SEQUENCE=AGACTGGGACAATGATGCCT
PRIMER_INTERNAL_0_SEQUENCE=TCTGGACAAACGTAGGGCTC
PRIMER_LEFT_0=69,20
PRIMER_RIGHT_0=153,20
PRIMER_INTERNAL_0=93,20
PRIMER_LEFT_0_TM=60.378
PRIMER_RIGHT_0_TM=59.535
PRIMER_INTERNAL_0_TM=60.255
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=0.842445
PRIMER_LEFT_1_PENALTY=0.377925
PRIMER_RIGHT_1_PENALTY=0.464520
PRIMER_INTERNAL_1_PENALTY=0.254613
PRIMER_LEFT_1_SEQUENCE=TGCAGCCTAAAAACTCCAGG
PRIMER_RIGHT_1_SEQUENCE=AGACTGGGACAATGATGCCT
PRIMER_INTERNAL_1_SEQUENCE=TCTGGACAAACGTAGGGCTC
PRIMER_LEFT_1=67,20
PRIMER_RIGHT_1=153,20
PRIMER_INTERNAL_1=93,20
PRIMER_LEFT_1_TM=60.378
PRIMER_RIGHT_1_TM=59.535
PRIMER_INTERNAL_1_TM=60.255
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=1.412615
PRIMER_LEFT_2_PENALTY=0.377925
PRIMER_RIGHT_2_PENALTY=1.034689
PRIMER_INTERNAL_2_PENALTY=0.254613
PRIMER_LEFT_2_SEQUENCE=CAGCCTAAAAACTCCAGGCA
PRIMER_RIGHT_2_SEQUENCE=TGGGACAATGATGCCTAAAA
PRIMER_INTERNAL_2_SEQUENCE=TCTGGACAAACGTAGGGCTC
PRIMER_LEFT_2=69,20
PRIMER_RIGHT_2=149,20
PRIMER_INTERNAL_2=93,20
PRIMER_LEFT_2_TM=60.378
PRIMER_RIGHT_2_TM=58.965
PRIMER_INTERNAL_2_TM=60.255
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=1.412615
PRIMER_LEFT_3_PENALTY=0.377925
PRIMER_RIGHT_3_PENALTY=1.034689
PRIMER_INTERNAL_3_PENALTY=0.254613
PRIMER_LEFT_3_SEQUENCE=TGCAGCCTAAAAACTCCAGG
PRIMER_RIGHT_3_SEQUENCE=TGGGACAATGATGCCTAAAA
PRIMER_INTERNAL_3_SEQUENCE=TCTGGACAAACGTAGGGCTC
PRIMER_LEFT_3=67,20
PRIMER_RIGHT_3=149,20
PRIMER_INTERNAL_3=93,20
PRIMER_LEFT_3_TM=60.378
PRIMER_RIGHT_3_TM=58.965
PRIMER_INTERNAL_3_TM=60.255
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=1.430622
PRIMER_LEFT_4_PENALTY=0.377925
PRIMER_RIGHT_4_PENALTY=1.052696
PRIMER_INTERNAL_4_PENALTY=0.254613
PRIMER_LEFT_4_SEQUENCE=CAGCCTAAAAACTCCAGGCA
PRIMER_RIGHT_4_SEQUENCE=GACTGGGACAATGATGCCTAA
PRIMER_INTERNAL_4_SEQUENCE=TCTGGACAAACGTAGGGCTC
PRIMER_LEFT_4=69,20
PRIMER_RIGHT_4=152,21
PRIMER_INTERNAL_4=93,20
PRIMER_LEFT_4_TM=60.378
PRIMER_RIGHT_4_TM=59.947
PRIMER_INTERNAL_4_TM=60.255
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH578
SEQUENCE_TEMPLATE=CCNNNNGTTCATGCTCAGGTCGACTCTAGAGGATCCCTGTGGGTTTCATCAGACCTGAGATTCAGCATGAAATCTACCAAAGGTACCACAAATGTAACCTTGTCCAAAACGAATCTCAGTTTCTGCATATGTAAAATGGGAATGATAAGAGCACCCACCTACCTCATGCAACTGTTGAGAGAAATAAATGAGACATTGTAAGTAAAGTTTGTAATGCACTGTTATGGCCTGAATTGTGTACCCTAAAATTCATATGTTGAAGCCCTAACACCCAATATGNCTGTATTTGTACATAAGGCCTTTAGGGNGGTAATTAAGGTTAAATG
SEQUENCE_INCLUDED_REGION=37,289
P3_COMMENT=CL is TCATCAGACCTGAGATTCAGC
P3_COMMENT=CR is TAAAGGCCTTATGTACAAATACAG
P3_COMMENT=CM is TGAAATCTACCAAAGGTACCACA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.334266
PRIMER_LEFT_0_PENALTY=0.010545
PRIMER_RIGHT_0_PENALTY=0.323721
PRIMER_INTERNAL_0_PENALTY=8.866785
PRIMER_LEFT_0_SEQUENCE=CCCACCTACCTCATGCAACT
PRIMER_RIGHT_0_SEQUENCE=GGGTACACAATTCAGGCCAT
PRIMER_INTERNAL_0_SEQUENCE=GTTGAGAGAAATAAATGAGACATTGT
PRIMER_LEFT_0=153,20
PRIMER_RIGHT_0=242,20
PRIMER_INTERNAL_0=173,26
PRIMER_LEFT_0_TM=59.989
PRIMER_RIGHT_0_TM=59.676
PRIMER_INTERNAL_0_TM=57.133
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=30.769
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=0.385635
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=0.375090
PRIMER_INTERNAL_1_PENALTY=8.866785
PRIMER_LEFT_1_SEQUENCE=CCCACCTACCTCATGCAACT
PRIMER_RIGHT_1_SEQUENCE=AGGGTACACAATTCAGGCCA
PRIMER_INTERNAL_1_SEQUENCE=GTTGAGAGAAATAAATGAGACATTGT
PRIMER_LEFT_1=153,20
PRIMER_RIGHT_1=243,20
PRIMER_INTERNAL_1=173,26
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.375
PRIMER_INTERNAL_1_TM=57.133
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=30.769
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.473115
PRIMER_LEFT_2_PENALTY=0.149395
PRIMER_RIGHT_2_PENALTY=0.323721
PRIMER_INTERNAL_2_PENALTY=7.198789
PRIMER_LEFT_2_SEQUENCE=ACCCACCTACCTCATGCAAC
PRIMER_RIGHT_2_SEQUENCE=GGGTACACAATTCAGGCCAT
PRIMER_INTERNAL_2_SEQUENCE=TGTTGAGAGAAATAAATGAGACATTG
PRIMER_LEFT_2=152,20
PRIMER_RIGHT_2=242,20
PRIMER_INTERNAL_2=172,26
PRIMER_LEFT_2_TM=59.851
PRIMER_RIGHT_2_TM=59.676
PRIMER_INTERNAL_2_TM=58.801
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=30.769
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_3_PENALTY=0.524485
PRIMER_LEFT_3_PENALTY=0.149395
PRIMER_RIGHT_3_PENALTY=0.375090
PRIMER_INTERNAL_3_PENALTY=7.198789
PRIMER_LEFT_3_SEQUENCE=ACCCACCTACCTCATGCAAC
PRIMER_RIGHT_3_SEQUENCE=AGGGTACACAATTCAGGCCA
PRIMER_INTERNAL_3_SEQUENCE=TGTTGAGAGAAATAAATGAGACATTG
PRIMER_LEFT_3=152,20
PRIMER_RIGHT_3=243,20
PRIMER_INTERNAL_3=172,26
PRIMER_LEFT_3_TM=59.851
PRIMER_RIGHT_3_TM=60.375
PRIMER_INTERNAL_3_TM=58.801
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=0.602568
PRIMER_LEFT_4_PENALTY=0.278847
PRIMER_RIGHT_4_PENALTY=0.323721
PRIMER_INTERNAL_4_PENALTY=5.173020
PRIMER_LEFT_4_SEQUENCE=AAGAGCACCCACCTACCTCA
PRIMER_RIGHT_4_SEQUENCE=GGGTACACAATTCAGGCCAT
PRIMER_INTERNAL_4_SEQUENCE=TGCAACTGTTGAGAGAAATAAATGA
PRIMER_LEFT_4=146,20
PRIMER_RIGHT_4=242,20
PRIMER_INTERNAL_4=166,25
PRIMER_LEFT_4_TM=59.721
PRIMER_RIGHT_4_TM=59.676
PRIMER_INTERNAL_4_TM=60.173
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=32.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=97
=
SEQUENCE_ID=MH584
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGAATTGACCCACAGCATCCCCACAGAATGTGATTTCTTGAGTCCTTTGTGAAGGTCACCTCCTCTAACCTCTCTATATATACATACTCAAACTTCTTTGGAGACTGGAAGATTGGAGGTACTATCTTTTAGAGTAGTTTTGCTGAAAAGTGATAAACTCCTTTCACAGTGTGGGAAAAGAGATCCAGTAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCT
SEQUENCE_INCLUDED_REGION=34,161
P3_COMMENT=CL is TCTGAATTGACCCACAGCAT
P3_COMMENT=CR is ACTTTTCAGCAAAACTACTCTAAAA
P3_COMMENT=CM is CCCACAGAATGTGATTTCTTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.948659
PRIMER_LEFT_0_PENALTY=1.604487
PRIMER_RIGHT_0_PENALTY=1.344173
PRIMER_INTERNAL_0_PENALTY=0.450843
PRIMER_LEFT_0_SEQUENCE=TCCCCACAGAATGTGATTTCT
PRIMER_RIGHT_0_SEQUENCE=TCCAATCTTCCAGTCTCCAAA
PRIMER_INTERNAL_0_SEQUENCE=CCTTTGTGAAGGTCACCTCC
PRIMER_LEFT_0=54,21
PRIMER_RIGHT_0=153,21
PRIMER_INTERNAL_0=80,20
PRIMER_LEFT_0_TM=59.396
PRIMER_RIGHT_0_TM=59.656
PRIMER_INTERNAL_0_TM=59.549
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=3.013129
PRIMER_LEFT_1_PENALTY=1.668956
PRIMER_RIGHT_1_PENALTY=1.344173
PRIMER_INTERNAL_1_PENALTY=0.450843
PRIMER_LEFT_1_SEQUENCE=TCCCCACAGAATGTGATTTC
PRIMER_RIGHT_1_SEQUENCE=TCCAATCTTCCAGTCTCCAAA
PRIMER_INTERNAL_1_SEQUENCE=CCTTTGTGAAGGTCACCTCC
PRIMER_LEFT_1=54,20
PRIMER_RIGHT_1=153,21
PRIMER_INTERNAL_1=80,20
PRIMER_LEFT_1_TM=58.331
PRIMER_RIGHT_1_TM=59.656
PRIMER_INTERNAL_1_TM=59.549
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=3.365079
PRIMER_LEFT_2_PENALTY=2.029253
PRIMER_RIGHT_2_PENALTY=1.335825
PRIMER_INTERNAL_2_PENALTY=0.450843
PRIMER_LEFT_2_SEQUENCE=CCCACAGAATGTGATTTCTTGA
PRIMER_RIGHT_2_SEQUENCE=CCTCCAATCTTCCAGTCTCC
PRIMER_INTERNAL_2_SEQUENCE=CCTTTGTGAAGGTCACCTCC
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=155,20
PRIMER_INTERNAL_2=80,20
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=58.664
PRIMER_INTERNAL_2_TM=59.549
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=7.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=3.373426
PRIMER_LEFT_3_PENALTY=2.029253
PRIMER_RIGHT_3_PENALTY=1.344173
PRIMER_INTERNAL_3_PENALTY=0.450843
PRIMER_LEFT_3_SEQUENCE=CCCACAGAATGTGATTTCTTGA
PRIMER_RIGHT_3_SEQUENCE=TCCAATCTTCCAGTCTCCAAA
PRIMER_INTERNAL_3_SEQUENCE=CCTTTGTGAAGGTCACCTCC
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=153,21
PRIMER_INTERNAL_3=80,20
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=59.656
PRIMER_INTERNAL_3_TM=59.549
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=3.403359
PRIMER_LEFT_4_PENALTY=1.604487
PRIMER_RIGHT_4_PENALTY=1.798872
PRIMER_INTERNAL_4_PENALTY=0.450843
PRIMER_LEFT_4_SEQUENCE=TCCCCACAGAATGTGATTTCT
PRIMER_RIGHT_4_SEQUENCE=TCCAATCTTCCAGTCTCCAA
PRIMER_INTERNAL_4_SEQUENCE=CCTTTGTGAAGGTCACCTCC
PRIMER_LEFT_4=54,21
PRIMER_RIGHT_4=153,20
PRIMER_INTERNAL_4=80,20
PRIMER_LEFT_4_TM=59.396
PRIMER_RIGHT_4_TM=58.201
PRIMER_INTERNAL_4_TM=59.549
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH585
SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCTGCACCAGATCTTTACCCTCTGGGGGGCTGAATCAACGAGTNCTAGACTGAAGCACCCAGGCACAGCCAAGGGTGAGGGAGCCATGATCCTGAACATAGCATTGACTGATTAATGAGACCCCCTGGGCCTGTCTTCAGTGCACTCCCAGTATGGGGACAGCCAGACTGGCGCACCCCCTCCGTGAGGCAGGAGACCCTAGGAGCGCTCTCTGGGGACAGTGACTGCAACTCTCGGTTCCCTTACAAATGGCTGAGTTGTCACCAGAAAATGACACTCACATATGGCAAACCCTGTCCT
SEQUENCE_INCLUDED_REGION=34,298
P3_COMMENT=CL is TGCACCAGATCTTTACCCTC
P3_COMMENT=CR is CTCAGCCATTTGTAAGGGAA
P3_COMMENT=CM is CTAGACTGAAGCACCCAGGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.268600
PRIMER_LEFT_0_PENALTY=0.012470
PRIMER_RIGHT_0_PENALTY=0.256130
PRIMER_INTERNAL_0_PENALTY=0.106742
PRIMER_LEFT_0_SEQUENCE=CTAGACTGAAGCACCCAGGC
PRIMER_RIGHT_0_SEQUENCE=CCAGGGGGTCTCATTAATCA
PRIMER_INTERNAL_0_SEQUENCE=GGAGCCATGATCCTGAACAT
PRIMER_LEFT_0=76,20
PRIMER_RIGHT_0=159,20
PRIMER_INTERNAL_0=111,20
PRIMER_LEFT_0_TM=60.012
PRIMER_RIGHT_0_TM=59.744
PRIMER_INTERNAL_0_TM=59.893
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=0.276782
PRIMER_LEFT_1_PENALTY=0.022009
PRIMER_RIGHT_1_PENALTY=0.254773
PRIMER_INTERNAL_1_PENALTY=1.359444
PRIMER_LEFT_1_SEQUENCE=TGTCTTCAGTGCACTCCCAG
PRIMER_RIGHT_1_SEQUENCE=AGAGAGCGCTCCTAGGGTCT
PRIMER_INTERNAL_1_SEQUENCE=TATGGGGACAGCCAGACTG
PRIMER_LEFT_1=163,20
PRIMER_RIGHT_1=244,20
PRIMER_INTERNAL_1=183,19
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=59.745
PRIMER_INTERNAL_1_TM=59.641
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.276782
PRIMER_LEFT_2_PENALTY=0.022009
PRIMER_RIGHT_2_PENALTY=0.254773
PRIMER_INTERNAL_2_PENALTY=0.532607
PRIMER_LEFT_2_SEQUENCE=CTGTCTTCAGTGCACTCCCA
PRIMER_RIGHT_2_SEQUENCE=AGAGAGCGCTCCTAGGGTCT
PRIMER_INTERNAL_2_SEQUENCE=GTATGGGGACAGCCAGACTG
PRIMER_LEFT_2=162,20
PRIMER_RIGHT_2=244,20
PRIMER_INTERNAL_2=182,20
PRIMER_LEFT_2_TM=60.022
PRIMER_RIGHT_2_TM=59.745
PRIMER_INTERNAL_2_TM=60.533
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.371384
PRIMER_LEFT_3_PENALTY=0.022009
PRIMER_RIGHT_3_PENALTY=0.349375
PRIMER_INTERNAL_3_PENALTY=0.532607
PRIMER_LEFT_3_SEQUENCE=CTGTCTTCAGTGCACTCCCA
PRIMER_RIGHT_3_SEQUENCE=CTCCTAGGGTCTCCTGCCTC
PRIMER_INTERNAL_3_SEQUENCE=GTATGGGGACAGCCAGACTG
PRIMER_LEFT_3=162,20
PRIMER_RIGHT_3=236,20
PRIMER_INTERNAL_3=182,20
PRIMER_LEFT_3_TM=60.022
PRIMER_RIGHT_3_TM=60.349
PRIMER_INTERNAL_3_TM=60.533
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=0.405684
PRIMER_LEFT_4_PENALTY=0.106742
PRIMER_RIGHT_4_PENALTY=0.298942
PRIMER_INTERNAL_4_PENALTY=0.256130
PRIMER_LEFT_4_SEQUENCE=GGAGCCATGATCCTGAACAT
PRIMER_RIGHT_4_SEQUENCE=CCATACTGGGAGTGCACTGA
PRIMER_INTERNAL_4_SEQUENCE=TGATTAATGAGACCCCCTGG
PRIMER_LEFT_4=111,20
PRIMER_RIGHT_4=187,20
PRIMER_INTERNAL_4=140,20
PRIMER_LEFT_4_TM=59.893
PRIMER_RIGHT_4_TM=59.701
PRIMER_INTERNAL_4_TM=59.744
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH590
SEQUENCE_TEMPLATE=GTTCATGCTGAGGTCGACTCTAGAGGATCCCCCTACCTTCATATACTATACTGTCTCTGTTGAAGGAGGAAATAGTGCAAAATAAACTACTTTGATATGCTGGATCTTGTTAATGAGTNATTCATCTAGTNAGCAAATCATTACTGTTCACCTTATGTGTGCCAGATATTACATCAACACTAGGGATCCTGTAGGTNNGGAGGGAAGTCCCTGCCTTTGTGAAACTTCAAATCAAATGTGGGAGACAGACACTAATCCAAAGAATTTCACCAGTAAG
SEQUENCE_INCLUDED_REGION=32,245
P3_COMMENT=CL is ACTATACTGTCTCTGTTGAAGGAGG
P3_COMMENT=CR is TGTCTGTCTCCCACATTTGA
P3_COMMENT=CM is AACTACTTTGATATGCTGGATCTTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.607801
PRIMER_LEFT_0_PENALTY=1.375502
PRIMER_RIGHT_0_PENALTY=0.232299
PRIMER_INTERNAL_0_PENALTY=3.799512
PRIMER_LEFT_0_SEQUENCE=TGTTCACCTTATGTGTGCCAG
PRIMER_RIGHT_0_SEQUENCE=TTCACAAAGGCAGGGACTTC
PRIMER_INTERNAL_0_SEQUENCE=TCAACACTAGGGATCCTGTAGG
PRIMER_LEFT_0=144,21
PRIMER_RIGHT_0=222,20
PRIMER_INTERNAL_0=173,22
PRIMER_LEFT_0_TM=59.624
PRIMER_RIGHT_0_TM=60.232
PRIMER_INTERNAL_0_TM=58.200
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=79
PRIMER_PAIR_1_PENALTY=1.607801
PRIMER_LEFT_1_PENALTY=1.375502
PRIMER_RIGHT_1_PENALTY=0.232299
PRIMER_INTERNAL_1_PENALTY=3.799512
PRIMER_LEFT_1_SEQUENCE=CTGTTCACCTTATGTGTGCCA
PRIMER_RIGHT_1_SEQUENCE=TTCACAAAGGCAGGGACTTC
PRIMER_INTERNAL_1_SEQUENCE=TCAACACTAGGGATCCTGTAGG
PRIMER_LEFT_1=143,21
PRIMER_RIGHT_1=222,20
PRIMER_INTERNAL_1=173,22
PRIMER_LEFT_1_TM=59.624
PRIMER_RIGHT_1_TM=60.232
PRIMER_INTERNAL_1_TM=58.200
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=1.664306
PRIMER_LEFT_2_PENALTY=1.375502
PRIMER_RIGHT_2_PENALTY=0.288804
PRIMER_INTERNAL_2_PENALTY=3.799512
PRIMER_LEFT_2_SEQUENCE=TGTTCACCTTATGTGTGCCAG
PRIMER_RIGHT_2_SEQUENCE=TTTCACAAAGGCAGGGACTT
PRIMER_INTERNAL_2_SEQUENCE=TCAACACTAGGGATCCTGTAGG
PRIMER_LEFT_2=144,21
PRIMER_RIGHT_2=223,20
PRIMER_INTERNAL_2=173,22
PRIMER_LEFT_2_TM=59.624
PRIMER_RIGHT_2_TM=59.711
PRIMER_INTERNAL_2_TM=58.200
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=1.664306
PRIMER_LEFT_3_PENALTY=1.375502
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_INTERNAL_3_PENALTY=3.799512
PRIMER_LEFT_3_SEQUENCE=TGTTCACCTTATGTGTGCCAG
PRIMER_RIGHT_3_SEQUENCE=AAGTTTCACAAAGGCAGGGA
PRIMER_INTERNAL_3_SEQUENCE=TCAACACTAGGGATCCTGTAGG
PRIMER_LEFT_3=144,21
PRIMER_RIGHT_3=226,20
PRIMER_INTERNAL_3=173,22
PRIMER_LEFT_3_TM=59.624
PRIMER_RIGHT_3_TM=59.711
PRIMER_INTERNAL_3_TM=58.200
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=1.664306
PRIMER_LEFT_4_PENALTY=1.375502
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_INTERNAL_4_PENALTY=3.799512
PRIMER_LEFT_4_SEQUENCE=TGTTCACCTTATGTGTGCCAG
PRIMER_RIGHT_4_SEQUENCE=GAAGTTTCACAAAGGCAGGG
PRIMER_INTERNAL_4_SEQUENCE=TCAACACTAGGGATCCTGTAGG
PRIMER_LEFT_4=144,21
PRIMER_RIGHT_4=227,20
PRIMER_INTERNAL_4=173,22
PRIMER_LEFT_4_TM=59.624
PRIMER_RIGHT_4_TM=59.711
PRIMER_INTERNAL_4_TM=58.200
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH592
SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGCAGTNNCCCCCACAGCAGGGTNCCACCTTTCAGAGTNNAAACAGGTCCTCACTGAACAGTCAATGTCACTTCCAGTCGGGGCACACTGTGTCCNAAGTTTACAATTNNAAACTCTGCTGTCTTGAGAAGNACTGTGGTTAATTCCTGGTACAAACCTTGCATCAGGAGAGTCCTATGCCATCTTCCACTAAAGCAATCAAGTGTGAACANCAGTNCAGAGGGGCTGGTCTGGCCTTAACAATCCTCAGATGGTGCCAGAGTCGGTAGATGCCAGGGGTAC
SEQUENCE_INCLUDED_REGION=33,221
P3_COMMENT=CL is AGTCAATGTCACTTCCAGTCG
P3_COMMENT=CR is GTTCACACTTGATTGCTTTAGTG
P3_COMMENT=CM is CATTGACTGTTCAGTGAGGACC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.907416
PRIMER_LEFT_0_PENALTY=2.165390
PRIMER_RIGHT_0_PENALTY=0.742026
PRIMER_INTERNAL_0_PENALTY=5.383533
PRIMER_LEFT_0_SEQUENCE=AGTCGGGGCACACTGTGT
PRIMER_RIGHT_0_SEQUENCE=GGACTCTCCTGATGCAAGGT
PRIMER_INTERNAL_0_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
PRIMER_LEFT_0=111,18
PRIMER_RIGHT_0=210,20
PRIMER_INTERNAL_0=168,23
PRIMER_LEFT_0_TM=60.165
PRIMER_RIGHT_0_TM=59.258
PRIMER_INTERNAL_0_TM=57.616
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=3.825155
PRIMER_LEFT_1_PENALTY=3.229947
PRIMER_RIGHT_1_PENALTY=0.595208
PRIMER_INTERNAL_1_PENALTY=4.885646
PRIMER_LEFT_1_SEQUENCE=GTCGGGGCACACTGTGTC
PRIMER_RIGHT_1_SEQUENCE=AGGACTCTCCTGATGCAAGG
PRIMER_INTERNAL_1_SEQUENCE=CTGTGGTTAATTCCTGGTACAAA
PRIMER_LEFT_1=112,18
PRIMER_RIGHT_1=211,20
PRIMER_INTERNAL_1=169,23
PRIMER_LEFT_1_TM=61.230
PRIMER_RIGHT_1_TM=59.405
PRIMER_INTERNAL_1_TM=58.114
PRIMER_LEFT_1_GC_PERCENT=66.667
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=3.971973
PRIMER_LEFT_2_PENALTY=3.229947
PRIMER_RIGHT_2_PENALTY=0.742026
PRIMER_INTERNAL_2_PENALTY=5.383533
PRIMER_LEFT_2_SEQUENCE=GTCGGGGCACACTGTGTC
PRIMER_RIGHT_2_SEQUENCE=GGACTCTCCTGATGCAAGGT
PRIMER_INTERNAL_2_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
PRIMER_LEFT_2=112,18
PRIMER_RIGHT_2=210,20
PRIMER_INTERNAL_2=168,23
PRIMER_LEFT_2_TM=61.230
PRIMER_RIGHT_2_TM=59.258
PRIMER_INTERNAL_2_TM=57.616
PRIMER_LEFT_2_GC_PERCENT=66.667
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=3.991869
PRIMER_LEFT_3_PENALTY=3.249843
PRIMER_RIGHT_3_PENALTY=0.742026
PRIMER_INTERNAL_3_PENALTY=5.383533
PRIMER_LEFT_3_SEQUENCE=AGTCGGGGCACACTGTGTC
PRIMER_RIGHT_3_SEQUENCE=GGACTCTCCTGATGCAAGGT
PRIMER_INTERNAL_3_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
PRIMER_LEFT_3=111,19
PRIMER_RIGHT_3=210,20
PRIMER_INTERNAL_3=168,23
PRIMER_LEFT_3_TM=62.250
PRIMER_RIGHT_3_TM=59.258
PRIMER_INTERNAL_3_TM=57.616
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=7.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=4.497249
PRIMER_LEFT_4_PENALTY=3.229947
PRIMER_RIGHT_4_PENALTY=1.267302
PRIMER_INTERNAL_4_PENALTY=5.383533
PRIMER_LEFT_4_SEQUENCE=GTCGGGGCACACTGTGTC
PRIMER_RIGHT_4_SEQUENCE=AGGACTCTCCTGATGCAAGGT
PRIMER_INTERNAL_4_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA
PRIMER_LEFT_4=112,18
PRIMER_RIGHT_4=211,21
PRIMER_INTERNAL_4=168,23
PRIMER_LEFT_4_TM=61.230
PRIMER_RIGHT_4_TM=60.267
PRIMER_INTERNAL_4_TM=57.616
PRIMER_LEFT_4_GC_PERCENT=66.667
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=7.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH599
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAGAGGNNNCTTATTTTTNCTTTTGTTAAAAAATCAATTTATTACTTTTAAAGTAGTTTTTGTAGATAAAGTTGCTAGAATATGTTGCTAAGCAGGTATGCTTTAAGANCTAGATAAGAAAAAAAATTTCTCATTATTAGGCAACAATTTGTATTGGTCGATTTGCAGCAACCAGAGAAGGAANCCAAACCACAATCCCTGCAGCAACCAGTCTGGGAAGTCAANCCACAATCTCTGCAATCATCAGCCCAGAATAATCAGGAAATGGCCAATGGCTGCCATATTTGTATTTGTTATGTAATTGTTGG
SEQUENCE_INCLUDED_REGION=36,312
P3_COMMENT=CL is AGTTGCTAGAATATGTTGCTAAGC
P3_COMMENT=CR is GGCTGATGATTGCAGAGATT
P3_COMMENT=CM is AAAAATTTCTCATTATTAGGCAACA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.404000
PRIMER_LEFT_0_PENALTY=0.215254
PRIMER_RIGHT_0_PENALTY=0.188746
PRIMER_INTERNAL_0_PENALTY=0.298018
PRIMER_LEFT_0_SEQUENCE=TATTGGTCGATTTGCAGCAA
PRIMER_RIGHT_0_SEQUENCE=GGGCTGATGATTGCAGAGAT
PRIMER_INTERNAL_0_SEQUENCE=CAGCAACCAGTCTGGGAAGT
PRIMER_LEFT_0=191,20
PRIMER_RIGHT_0=289,20
PRIMER_INTERNAL_0=241,20
PRIMER_LEFT_0_TM=60.215
PRIMER_RIGHT_0_TM=60.189
PRIMER_INTERNAL_0_TM=60.298
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=0.494699
PRIMER_LEFT_1_PENALTY=0.305953
PRIMER_RIGHT_1_PENALTY=0.188746
PRIMER_INTERNAL_1_PENALTY=0.298018
PRIMER_LEFT_1_SEQUENCE=GTATTGGTCGATTTGCAGCA
PRIMER_RIGHT_1_SEQUENCE=GGGCTGATGATTGCAGAGAT
PRIMER_INTERNAL_1_SEQUENCE=CAGCAACCAGTCTGGGAAGT
PRIMER_LEFT_1=190,20
PRIMER_RIGHT_1=289,20
PRIMER_INTERNAL_1=241,20
PRIMER_LEFT_1_TM=59.694
PRIMER_RIGHT_1_TM=60.189
PRIMER_INTERNAL_1_TM=60.298
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=1.019458
PRIMER_LEFT_2_PENALTY=0.298018
PRIMER_RIGHT_2_PENALTY=0.721440
PRIMER_INTERNAL_2_PENALTY=0.188746
PRIMER_LEFT_2_SEQUENCE=CAGCAACCAGTCTGGGAAGT
PRIMER_RIGHT_2_SEQUENCE=ACAAATATGGCAGCCATTGG
PRIMER_INTERNAL_2_SEQUENCE=ATCTCTGCAATCATCAGCCC
PRIMER_LEFT_2=241,20
PRIMER_RIGHT_2=327,20
PRIMER_INTERNAL_2=270,20
PRIMER_LEFT_2_TM=60.298
PRIMER_RIGHT_2_TM=60.721
PRIMER_INTERNAL_2_TM=60.189
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=9.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=1.186911
PRIMER_LEFT_3_PENALTY=0.998165
PRIMER_RIGHT_3_PENALTY=0.188746
PRIMER_INTERNAL_3_PENALTY=0.298018
PRIMER_LEFT_3_SEQUENCE=GATTTGCAGCAACCAGAGAA
PRIMER_RIGHT_3_SEQUENCE=GGGCTGATGATTGCAGAGAT
PRIMER_INTERNAL_3_SEQUENCE=CAGCAACCAGTCTGGGAAGT
PRIMER_LEFT_3=199,20
PRIMER_RIGHT_3=289,20
PRIMER_INTERNAL_3=241,20
PRIMER_LEFT_3_TM=59.002
PRIMER_RIGHT_3_TM=60.189
PRIMER_INTERNAL_3_TM=60.298
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=1.196415
PRIMER_LEFT_4_PENALTY=1.007669
PRIMER_RIGHT_4_PENALTY=0.188746
PRIMER_INTERNAL_4_PENALTY=0.298018
PRIMER_LEFT_4_SEQUENCE=GATTTGCAGCAACCAGAGAAG
PRIMER_RIGHT_4_SEQUENCE=GGGCTGATGATTGCAGAGAT
PRIMER_INTERNAL_4_SEQUENCE=CAGCAACCAGTCTGGGAAGT
PRIMER_LEFT_4=199,21
PRIMER_RIGHT_4=289,20
PRIMER_INTERNAL_4=241,20
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=60.189
PRIMER_INTERNAL_4_TM=60.298
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH604
SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGTNNATCGCTTCCTCAGAGANNCCGTCCCTGACTCCCAGACAAGGNCGGGCTGGGCCTTTTGTATGTNNTCAGAGCACCCTTGGTGATTCCTTAAGCACTTAGCACAATTTGTAATCATACACCTATTTCTGCAATTATTTGTTGTAGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA
SEQUENCE_INCLUDED_REGION=36,118
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.611000
PRIMER_LEFT_0_PENALTY=0.317911
PRIMER_RIGHT_0_PENALTY=2.293089
PRIMER_INTERNAL_0_PENALTY=1.052696
PRIMER_LEFT_0_SEQUENCE=GTCCCTGACTCCCAGACAAG
PRIMER_RIGHT_0_SEQUENCE=GTGCTTAAGGAATCACCAAGG
PRIMER_INTERNAL_0_SEQUENCE=GGCTGGGCCTTTTGTATGT
PRIMER_LEFT_0=65,20
PRIMER_RIGHT_0=139,21
PRIMER_INTERNAL_0=89,19
PRIMER_LEFT_0_TM=59.682
PRIMER_RIGHT_0_TM=58.707
PRIMER_INTERNAL_0_TM=59.947
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=52.632
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=2.665040
PRIMER_LEFT_1_PENALTY=0.317911
PRIMER_RIGHT_1_PENALTY=2.347129
PRIMER_INTERNAL_1_PENALTY=1.052696
PRIMER_LEFT_1_SEQUENCE=GTCCCTGACTCCCAGACAAG
PRIMER_RIGHT_1_SEQUENCE=AGTGCTTAAGGAATCACCAAGG
PRIMER_INTERNAL_1_SEQUENCE=GGCTGGGCCTTTTGTATGT
PRIMER_LEFT_1=65,20
PRIMER_RIGHT_1=140,22
PRIMER_INTERNAL_1=89,19
PRIMER_LEFT_1_TM=59.682
PRIMER_RIGHT_1_TM=59.653
PRIMER_INTERNAL_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=52.632
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=3.511807
PRIMER_LEFT_2_PENALTY=0.317911
PRIMER_RIGHT_2_PENALTY=3.193896
PRIMER_INTERNAL_2_PENALTY=1.052696
PRIMER_LEFT_2_SEQUENCE=GTCCCTGACTCCCAGACAAG
PRIMER_RIGHT_2_SEQUENCE=GCTAAGTGCTTAAGGAATCACCA
PRIMER_INTERNAL_2_SEQUENCE=GGCTGGGCCTTTTGTATGT
PRIMER_LEFT_2=65,20
PRIMER_RIGHT_2=144,23
PRIMER_INTERNAL_2=89,19
PRIMER_LEFT_2_TM=59.682
PRIMER_RIGHT_2_TM=59.806
PRIMER_INTERNAL_2_TM=59.947
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_INTERNAL_2_GC_PERCENT=52.632
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=3.511807
PRIMER_LEFT_3_PENALTY=0.317911
PRIMER_RIGHT_3_PENALTY=3.193896
PRIMER_INTERNAL_3_PENALTY=1.052696
PRIMER_LEFT_3_SEQUENCE=GTCCCTGACTCCCAGACAAG
PRIMER_RIGHT_3_SEQUENCE=TGCTAAGTGCTTAAGGAATCACC
PRIMER_INTERNAL_3_SEQUENCE=GGCTGGGCCTTTTGTATGT
PRIMER_LEFT_3=65,20
PRIMER_RIGHT_3=145,23
PRIMER_INTERNAL_3=89,19
PRIMER_LEFT_3_TM=59.682
PRIMER_RIGHT_3_TM=59.806
PRIMER_INTERNAL_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_INTERNAL_3_GC_PERCENT=52.632
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=4.164937
PRIMER_LEFT_4_PENALTY=0.317911
PRIMER_RIGHT_4_PENALTY=3.847026
PRIMER_INTERNAL_4_PENALTY=1.052696
PRIMER_LEFT_4_SEQUENCE=GTCCCTGACTCCCAGACAAG
PRIMER_RIGHT_4_SEQUENCE=AAGTGCTTAAGGAATCACCAAGG
PRIMER_INTERNAL_4_SEQUENCE=GGCTGGGCCTTTTGTATGT
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=141,23
PRIMER_INTERNAL_4=89,19
PRIMER_LEFT_4_TM=59.682
PRIMER_RIGHT_4_TM=60.847
PRIMER_INTERNAL_4_TM=59.947
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_INTERNAL_4_GC_PERCENT=52.632
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH618
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAAGCGTAAGAATACGTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
SEQUENCE_INCLUDED_REGION=35,21
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH623
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTTCATAGTNNGCGTNNTACAAAACAACTTAGTNTTCACATTCAATTCAGTAATTAACATGGTGTTTTNTTCCAGTGAACAGAAAAGTTATTATAAAACAATATATTTCCATTCGTCATTGTCTTTTCTCACTTCTGACTAGCCACAAGGTCTTGACAACTTTTTTTCTATGTAAAATGAGACTGTGCTATGAATATCTCAGAGG
SEQUENCE_INCLUDED_REGION=34,206
P3_COMMENT=CL is ACATTCAATTCAGTAATTAACATGG
P3_COMMENT=CR is TTCATAGCACAGTCTCATTTTACA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=8.948001
PRIMER_LEFT_0_PENALTY=2.461740
PRIMER_RIGHT_0_PENALTY=6.486261
PRIMER_INTERNAL_0_PENALTY=9.320856
PRIMER_LEFT_0_SEQUENCE=TCACTTCTGACTAGCCACAAGG
PRIMER_RIGHT_0_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
PRIMER_INTERNAL_0_SEQUENCE=TGACAACTTTTTTTCTATGTAAAATGA
PRIMER_LEFT_0=163,22
PRIMER_RIGHT_0=239,25
PRIMER_INTERNAL_0=188,27
PRIMER_LEFT_0_TM=59.538
PRIMER_RIGHT_0_TM=58.514
PRIMER_INTERNAL_0_TM=57.679
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=44.000
PRIMER_INTERNAL_0_GC_PERCENT=22.222
PRIMER_LEFT_0_SELF_ANY=10.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=9.419196
PRIMER_LEFT_1_PENALTY=2.932936
PRIMER_RIGHT_1_PENALTY=6.486261
PRIMER_INTERNAL_1_PENALTY=1.753359
PRIMER_LEFT_1_SEQUENCE=TTCCATTCGTCATTGTCTTTTC
PRIMER_RIGHT_1_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
PRIMER_INTERNAL_1_SEQUENCE=GCCACAAGGTCTTGACAACTT
PRIMER_LEFT_1=141,22
PRIMER_RIGHT_1=239,25
PRIMER_INTERNAL_1=176,21
PRIMER_LEFT_1_TM=59.067
PRIMER_RIGHT_1_TM=58.514
PRIMER_INTERNAL_1_TM=59.247
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=44.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=9.492569
PRIMER_LEFT_2_PENALTY=3.006308
PRIMER_RIGHT_2_PENALTY=6.486261
PRIMER_INTERNAL_2_PENALTY=1.753359
PRIMER_LEFT_2_SEQUENCE=TTCCATTCGTCATTGTCTTTTCT
PRIMER_RIGHT_2_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
PRIMER_INTERNAL_2_SEQUENCE=GCCACAAGGTCTTGACAACTT
PRIMER_LEFT_2=141,23
PRIMER_RIGHT_2=239,25
PRIMER_INTERNAL_2=176,21
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=58.514
PRIMER_INTERNAL_2_TM=59.247
PRIMER_LEFT_2_GC_PERCENT=34.783
PRIMER_RIGHT_2_GC_PERCENT=44.000
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=9.790787
PRIMER_LEFT_3_PENALTY=3.304526
PRIMER_RIGHT_3_PENALTY=6.486261
PRIMER_INTERNAL_3_PENALTY=1.753359
PRIMER_LEFT_3_SEQUENCE=CCATTCGTCATTGTCTTTTCTC
PRIMER_RIGHT_3_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
PRIMER_INTERNAL_3_SEQUENCE=GCCACAAGGTCTTGACAACTT
PRIMER_LEFT_3=143,22
PRIMER_RIGHT_3=239,25
PRIMER_INTERNAL_3=176,21
PRIMER_LEFT_3_TM=58.695
PRIMER_RIGHT_3_TM=58.514
PRIMER_INTERNAL_3_TM=59.247
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=44.000
PRIMER_INTERNAL_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=9.790787
PRIMER_LEFT_4_PENALTY=3.304526
PRIMER_RIGHT_4_PENALTY=6.486261
PRIMER_INTERNAL_4_PENALTY=1.753359
PRIMER_LEFT_4_SEQUENCE=TCCATTCGTCATTGTCTTTTCT
PRIMER_RIGHT_4_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC
PRIMER_INTERNAL_4_SEQUENCE=GCCACAAGGTCTTGACAACTT
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=239,25
PRIMER_INTERNAL_4=176,21
PRIMER_LEFT_4_TM=58.695
PRIMER_RIGHT_4_TM=58.514
PRIMER_INTERNAL_4_TM=59.247
PRIMER_LEFT_4_GC_PERCENT=36.364
PRIMER_RIGHT_4_GC_PERCENT=44.000
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH629
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTGCCCACAGCAGATGGTGACAGGGTCTACCAGGTAGTTCAAACAGATGACGCAGGTGAGTTCCTTCTGGAAGGCATGTGAGAAGTCTGAGTCCATTTTCCTAAGGAAAGAAANCCACAGGAATTTAATCTTCTACCCTGGAGAGACAAAGATCCAAGCAAAGTTTGAATCAGATCGTGATCGAATAATATCCTTTCTTTCTAAAGAAGTATAGGCTTTAATCTGCA
SEQUENCE_INCLUDED_REGION=33,228
P3_COMMENT=CL is AACAGATGACGCAGGTGAGT
P3_COMMENT=CR is AAGAAAGGATATTATTCGATCACG
P3_COMMENT=CM is CCTTCTGGAAGGCATGTGAG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.917835
PRIMER_LEFT_0_PENALTY=0.286624
PRIMER_RIGHT_0_PENALTY=0.631211
PRIMER_INTERNAL_0_PENALTY=0.269335
PRIMER_LEFT_0_SEQUENCE=AGCAGATGGTGACAGGGTCT
PRIMER_RIGHT_0_SEQUENCE=TTCTCACATGCCTTCCAGAA
PRIMER_INTERNAL_0_SEQUENCE=AGATGACGCAGGTGAGTTCC
PRIMER_LEFT_0=42,20
PRIMER_RIGHT_0=117,20
PRIMER_INTERNAL_0=78,20
PRIMER_LEFT_0_TM=59.713
PRIMER_RIGHT_0_TM=59.369
PRIMER_INTERNAL_0_TM=60.269
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=1.226796
PRIMER_LEFT_1_PENALTY=0.595585
PRIMER_RIGHT_1_PENALTY=0.631211
PRIMER_INTERNAL_1_PENALTY=0.269335
PRIMER_LEFT_1_SEQUENCE=ACAGCAGATGGTGACAGGGT
PRIMER_RIGHT_1_SEQUENCE=TTCTCACATGCCTTCCAGAA
PRIMER_INTERNAL_1_SEQUENCE=AGATGACGCAGGTGAGTTCC
PRIMER_LEFT_1=40,20
PRIMER_RIGHT_1=117,20
PRIMER_INTERNAL_1=78,20
PRIMER_LEFT_1_TM=60.596
PRIMER_RIGHT_1_TM=59.369
PRIMER_INTERNAL_1_TM=60.269
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=1.329902
PRIMER_LEFT_2_PENALTY=0.286624
PRIMER_RIGHT_2_PENALTY=1.043279
PRIMER_INTERNAL_2_PENALTY=0.580148
PRIMER_LEFT_2_SEQUENCE=AGCAGATGGTGACAGGGTCT
PRIMER_RIGHT_2_SEQUENCE=TCTCACATGCCTTCCAGAAG
PRIMER_INTERNAL_2_SEQUENCE=CAGATGACGCAGGTGAGTTC
PRIMER_LEFT_2=42,20
PRIMER_RIGHT_2=116,20
PRIMER_INTERNAL_2=77,20
PRIMER_LEFT_2_TM=59.713
PRIMER_RIGHT_2_TM=58.957
PRIMER_INTERNAL_2_TM=59.420
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=1.329902
PRIMER_LEFT_3_PENALTY=0.286624
PRIMER_RIGHT_3_PENALTY=1.043279
PRIMER_INTERNAL_3_PENALTY=0.269335
PRIMER_LEFT_3_SEQUENCE=AGCAGATGGTGACAGGGTCT
PRIMER_RIGHT_3_SEQUENCE=CTTCTCACATGCCTTCCAGA
PRIMER_INTERNAL_3_SEQUENCE=GATGACGCAGGTGAGTTCCT
PRIMER_LEFT_3=42,20
PRIMER_RIGHT_3=118,20
PRIMER_INTERNAL_3=79,20
PRIMER_LEFT_3_TM=59.713
PRIMER_RIGHT_3_TM=58.957
PRIMER_INTERNAL_3_TM=60.269
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=1.386172
PRIMER_LEFT_4_PENALTY=0.595585
PRIMER_RIGHT_4_PENALTY=0.790588
PRIMER_INTERNAL_4_PENALTY=0.707326
PRIMER_LEFT_4_SEQUENCE=ACAGCAGATGGTGACAGGGT
PRIMER_RIGHT_4_SEQUENCE=CTCACATGCCTTCCAGAAGG
PRIMER_INTERNAL_4_SEQUENCE=TTCAAACAGATGACGCAGGT
PRIMER_LEFT_4=40,20
PRIMER_RIGHT_4=115,20
PRIMER_INTERNAL_4=71,20
PRIMER_LEFT_4_TM=60.596
PRIMER_RIGHT_4_TM=60.791
PRIMER_INTERNAL_4_TM=59.293
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=8.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH633
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCCAAAGAAAAGTGACTAAATCAAGAAGGTTAACTCACATCCCTGCCTGGGAACCGNGANGTCCTGGCCTATAGTAACAAAAATTTCTCTTTCATGCAATGTCTACTCTATTGCAGGCACAGGGCTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATG
SEQUENCE_INCLUDED_REGION=33,132
P3_COMMENT=CL is CTTCCAAAGAAAAGTGACTAAATC
P3_COMMENT=CR is CTAGCCCTGTGCCTGCAATA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.866715
PRIMER_LEFT_0_PENALTY=0.589903
PRIMER_RIGHT_0_PENALTY=0.276812
PRIMER_INTERNAL_0_PENALTY=4.006288
PRIMER_LEFT_0_SEQUENCE=GGTTAACTCACATCCCTGCC
PRIMER_RIGHT_0_SEQUENCE=CCCTGTGCCTGCAATAGAGT
PRIMER_INTERNAL_0_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
PRIMER_LEFT_0=62,20
PRIMER_RIGHT_0=158,20
PRIMER_INTERNAL_0=112,24
PRIMER_LEFT_0_TM=59.410
PRIMER_RIGHT_0_TM=60.277
PRIMER_INTERNAL_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=1.331768
PRIMER_LEFT_1_PENALTY=1.054956
PRIMER_RIGHT_1_PENALTY=0.276812
PRIMER_INTERNAL_1_PENALTY=4.006288
PRIMER_LEFT_1_SEQUENCE=TAACTCACATCCCTGCCTGG
PRIMER_RIGHT_1_SEQUENCE=CCCTGTGCCTGCAATAGAGT
PRIMER_INTERNAL_1_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
PRIMER_LEFT_1=65,20
PRIMER_RIGHT_1=158,20
PRIMER_INTERNAL_1=112,24
PRIMER_LEFT_1_TM=61.055
PRIMER_RIGHT_1_TM=60.277
PRIMER_INTERNAL_1_TM=60.006
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=29.167
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=1.554978
PRIMER_LEFT_2_PENALTY=1.278166
PRIMER_RIGHT_2_PENALTY=0.276812
PRIMER_INTERNAL_2_PENALTY=4.006288
PRIMER_LEFT_2_SEQUENCE=TTAACTCACATCCCTGCCTG
PRIMER_RIGHT_2_SEQUENCE=CCCTGTGCCTGCAATAGAGT
PRIMER_INTERNAL_2_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
PRIMER_LEFT_2=64,20
PRIMER_RIGHT_2=158,20
PRIMER_INTERNAL_2=112,24
PRIMER_LEFT_2_TM=58.722
PRIMER_RIGHT_2_TM=60.277
PRIMER_INTERNAL_2_TM=60.006
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=1.652760
PRIMER_LEFT_3_PENALTY=1.375948
PRIMER_RIGHT_3_PENALTY=0.276812
PRIMER_INTERNAL_3_PENALTY=4.006288
PRIMER_LEFT_3_SEQUENCE=GGTTAACTCACATCCCTGCCT
PRIMER_RIGHT_3_SEQUENCE=CCCTGTGCCTGCAATAGAGT
PRIMER_INTERNAL_3_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
PRIMER_LEFT_3=62,21
PRIMER_RIGHT_3=158,20
PRIMER_INTERNAL_3=112,24
PRIMER_LEFT_3_TM=60.376
PRIMER_RIGHT_3_TM=60.277
PRIMER_INTERNAL_3_TM=60.006
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=1.652760
PRIMER_LEFT_4_PENALTY=1.375948
PRIMER_RIGHT_4_PENALTY=0.276812
PRIMER_INTERNAL_4_PENALTY=4.006288
PRIMER_LEFT_4_SEQUENCE=AGGTTAACTCACATCCCTGCC
PRIMER_RIGHT_4_SEQUENCE=CCCTGTGCCTGCAATAGAGT
PRIMER_INTERNAL_4_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG
PRIMER_LEFT_4=61,21
PRIMER_RIGHT_4=158,20
PRIMER_INTERNAL_4=112,24
PRIMER_LEFT_4_TM=60.376
PRIMER_RIGHT_4_TM=60.277
PRIMER_INTERNAL_4_TM=60.006
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=29.167
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH636
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATCCCCCATCCCAGCCCACAACCATCTCACTGATGAGTCTGTAACATGAAACTAAATCAAAAAGATCCCCAACAGAATTAGAAAAAATATTAAACTCACTGCTTGCTTGTTGATTTAATCAACCTAGCCGGCTGTCATGTGGGATTAGAATAAAATAAACACAAAAATGAAAACACACGATTGCTAACAAAGCAGATTCTTTTTTCAAGGCACACGTAAAGATAATAACTTCAAAGTTGATTATATTAACTTGTCTTCCCATGATAACACG
SEQUENCE_INCLUDED_REGION=36,276
P3_COMMENT=CL is ACTCACTGCTTGCTTGTTGA
P3_COMMENT=CR is TTGAAGTTATTATCTTTACGTGTGC
P3_COMMENT=CM is TTAATCAACCTAGCCGGCTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.296956
PRIMER_LEFT_0_PENALTY=0.222749
PRIMER_RIGHT_0_PENALTY=1.074208
PRIMER_INTERNAL_0_PENALTY=7.771682
PRIMER_LEFT_0_SEQUENCE=TTAATCAACCTAGCCGGCTG
PRIMER_RIGHT_0_SEQUENCE=TGCTTTGTTAGCAATCGTGTG
PRIMER_INTERNAL_0_SEQUENCE=TGTGGGATTAGAATAAAATAAACACAA
PRIMER_LEFT_0=155,20
PRIMER_RIGHT_0=234,21
PRIMER_INTERNAL_0=178,27
PRIMER_LEFT_0_TM=60.223
PRIMER_RIGHT_0_TM=59.926
PRIMER_INTERNAL_0_TM=59.228
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=25.926
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=1.344175
PRIMER_LEFT_1_PENALTY=0.269968
PRIMER_RIGHT_1_PENALTY=1.074208
PRIMER_INTERNAL_1_PENALTY=7.771682
PRIMER_LEFT_1_SEQUENCE=TAATCAACCTAGCCGGCTGT
PRIMER_RIGHT_1_SEQUENCE=TGCTTTGTTAGCAATCGTGTG
PRIMER_INTERNAL_1_SEQUENCE=TGTGGGATTAGAATAAAATAAACACAA
PRIMER_LEFT_1=156,20
PRIMER_RIGHT_1=234,21
PRIMER_INTERNAL_1=178,27
PRIMER_LEFT_1_TM=59.730
PRIMER_RIGHT_1_TM=59.926
PRIMER_INTERNAL_1_TM=59.228
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_INTERNAL_1_GC_PERCENT=25.926
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=1.690886
PRIMER_LEFT_2_PENALTY=1.468137
PRIMER_RIGHT_2_PENALTY=0.222749
PRIMER_INTERNAL_2_PENALTY=2.095684
PRIMER_LEFT_2_SEQUENCE=TCAAAAAGATCCCCAACAGAA
PRIMER_RIGHT_2_SEQUENCE=CAGCCGGCTAGGTTGATTAA
PRIMER_INTERNAL_2_SEQUENCE=AAACTCACTGCTTGCTTGTTGA
PRIMER_LEFT_2=97,21
PRIMER_RIGHT_2=174,20
PRIMER_INTERNAL_2=132,22
PRIMER_LEFT_2_TM=59.532
PRIMER_RIGHT_2_TM=60.223
PRIMER_INTERNAL_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=1.738105
PRIMER_LEFT_3_PENALTY=1.468137
PRIMER_RIGHT_3_PENALTY=0.269968
PRIMER_INTERNAL_3_PENALTY=2.095684
PRIMER_LEFT_3_SEQUENCE=TCAAAAAGATCCCCAACAGAA
PRIMER_RIGHT_3_SEQUENCE=ACAGCCGGCTAGGTTGATTA
PRIMER_INTERNAL_3_SEQUENCE=AAACTCACTGCTTGCTTGTTGA
PRIMER_LEFT_3=97,21
PRIMER_RIGHT_3=175,20
PRIMER_INTERNAL_3=132,22
PRIMER_LEFT_3_TM=59.532
PRIMER_RIGHT_3_TM=59.730
PRIMER_INTERNAL_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=1.757691
PRIMER_LEFT_4_PENALTY=0.222749
PRIMER_RIGHT_4_PENALTY=1.534943
PRIMER_INTERNAL_4_PENALTY=2.725715
PRIMER_LEFT_4_SEQUENCE=TTAATCAACCTAGCCGGCTG
PRIMER_RIGHT_4_SEQUENCE=TGCCTTGAAAAAAGAATCTGC
PRIMER_INTERNAL_4_SEQUENCE=TGAAAACACACGATTGCTAACA
PRIMER_LEFT_4=155,20
PRIMER_RIGHT_4=252,21
PRIMER_INTERNAL_4=208,22
PRIMER_LEFT_4_TM=60.223
PRIMER_RIGHT_4_TM=59.465
PRIMER_INTERNAL_4_TM=59.274
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=36.364
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH640
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTATAATCCACACACCTTGAATTAAATAGCACGTGAGAGGGNTCCTGGGGTAAATGAGCATTTAATCACTTTTCATACCCTTTTTATTGTCAAGGGAATGAACAGGGTCTGGAAAAGTGGTGAGGTATTTTGTTTTACTGCAGTTGCTATTTAGATTCATGGAAGTTTGAATAATGACTATTACTGAAAAAGATTGCTTTGGCCAGTTTAGATATTAAGAAAAAACCTGCAGAAAGATTATGG
SEQUENCE_INCLUDED_REGION=35,242
P3_COMMENT=CL is AATTAAATAGCACGTGAGAGGG
P3_COMMENT=CR is TTTTTTCTTAATATCTAAACTGGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.639098
PRIMER_LEFT_0_PENALTY=0.450843
PRIMER_RIGHT_0_PENALTY=0.188254
PRIMER_INTERNAL_0_PENALTY=4.330336
PRIMER_LEFT_0_SEQUENCE=GGTCTGGAAAAGTGGTGAGG
PRIMER_RIGHT_0_SEQUENCE=GGCCAAAGCAATCTTTTTCA
PRIMER_INTERNAL_0_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA
PRIMER_LEFT_0=139,20
PRIMER_RIGHT_0=237,20
PRIMER_INTERNAL_0=173,24
PRIMER_LEFT_0_TM=59.549
PRIMER_RIGHT_0_TM=60.188
PRIMER_INTERNAL_0_TM=59.670
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=0.639098
PRIMER_LEFT_1_PENALTY=0.450843
PRIMER_RIGHT_1_PENALTY=0.188254
PRIMER_INTERNAL_1_PENALTY=4.330336
PRIMER_LEFT_1_SEQUENCE=GGTCTGGAAAAGTGGTGAGG
PRIMER_RIGHT_1_SEQUENCE=TGGCCAAAGCAATCTTTTTC
PRIMER_INTERNAL_1_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA
PRIMER_LEFT_1=139,20
PRIMER_RIGHT_1=238,20
PRIMER_INTERNAL_1=173,24
PRIMER_LEFT_1_TM=59.549
PRIMER_RIGHT_1_TM=60.188
PRIMER_INTERNAL_1_TM=59.670
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=0.639098
PRIMER_LEFT_2_PENALTY=0.450843
PRIMER_RIGHT_2_PENALTY=0.188254
PRIMER_INTERNAL_2_PENALTY=4.330336
PRIMER_LEFT_2_SEQUENCE=GGGTCTGGAAAAGTGGTGAG
PRIMER_RIGHT_2_SEQUENCE=GGCCAAAGCAATCTTTTTCA
PRIMER_INTERNAL_2_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA
PRIMER_LEFT_2=138,20
PRIMER_RIGHT_2=237,20
PRIMER_INTERNAL_2=173,24
PRIMER_LEFT_2_TM=59.549
PRIMER_RIGHT_2_TM=60.188
PRIMER_INTERNAL_2_TM=59.670
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=1.064062
PRIMER_LEFT_3_PENALTY=0.613219
PRIMER_RIGHT_3_PENALTY=0.450843
PRIMER_INTERNAL_3_PENALTY=2.899153
PRIMER_LEFT_3_SEQUENCE=TCCTGGGGTAAATGAGCATT
PRIMER_RIGHT_3_SEQUENCE=TCACCACTTTTCCAGACCCT
PRIMER_INTERNAL_3_SEQUENCE=CCCTTTTTATTGTCAAGGGAA
PRIMER_LEFT_3=76,20
PRIMER_RIGHT_3=156,20
PRIMER_INTERNAL_3=111,21
PRIMER_LEFT_3_TM=59.387
PRIMER_RIGHT_3_TM=59.549
PRIMER_INTERNAL_3_TM=58.101
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=1.064062
PRIMER_LEFT_4_PENALTY=0.613219
PRIMER_RIGHT_4_PENALTY=0.450843
PRIMER_INTERNAL_4_PENALTY=2.899153
PRIMER_LEFT_4_SEQUENCE=TCCTGGGGTAAATGAGCATT
PRIMER_RIGHT_4_SEQUENCE=CTCACCACTTTTCCAGACCC
PRIMER_INTERNAL_4_SEQUENCE=CCCTTTTTATTGTCAAGGGAA
PRIMER_LEFT_4=76,20
PRIMER_RIGHT_4=157,20
PRIMER_INTERNAL_4=111,21
PRIMER_LEFT_4_TM=59.387
PRIMER_RIGHT_4_TM=59.549
PRIMER_INTERNAL_4_TM=58.101
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH642
SEQUENCE_TEMPLATE=AACCTAAATTTTGCAAAGCAGTCTGGTCATATCGGAAAATATGCCTTTCTAGCAAAGCCTTGGTAAAATCAGAAGTGTTTCAAATTATGTCCTATTACTAAAGAGAACAAATTNTTACCCAACTTATGCAAATAACTGTATTGCCATGAGTTAAGAATACCCACAAATAGTTTCTCAATTTTGGAGGAATGAGGCAGAGAGAAATAGAAATTCTTCAGATTTGGCTAACAGAAGTATACTTTATCCAATGTTTTGTTAG
SEQUENCE_INCLUDED_REGION=0,259
P3_COMMENT=CL is TGCAAAGCAGTCTGGTCATA
P3_COMMENT=CR is CTGAAGAATTTCTATTTCTCTCTGC
P3_COMMENT=CM is CGGAAAATATGCCTTTCTAGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.854017
PRIMER_LEFT_0_PENALTY=3.366081
PRIMER_RIGHT_0_PENALTY=0.487936
PRIMER_INTERNAL_0_PENALTY=7.324192
PRIMER_LEFT_0_SEQUENCE=TGCAAATAACTGTATTGCCATG
PRIMER_RIGHT_0_SEQUENCE=CTCTCTGCCTCATTCCTCCA
PRIMER_INTERNAL_0_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA
PRIMER_LEFT_0=126,22
PRIMER_RIGHT_0=200,20
PRIMER_INTERNAL_0=152,26
PRIMER_LEFT_0_TM=58.634
PRIMER_RIGHT_0_TM=60.488
PRIMER_INTERNAL_0_TM=58.676
PRIMER_LEFT_0_GC_PERCENT=36.364
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=30.769
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=3.868756
PRIMER_LEFT_1_PENALTY=3.380820
PRIMER_RIGHT_1_PENALTY=0.487936
PRIMER_INTERNAL_1_PENALTY=7.324192
PRIMER_LEFT_1_SEQUENCE=TGCAAATAACTGTATTGCCATGA
PRIMER_RIGHT_1_SEQUENCE=CTCTCTGCCTCATTCCTCCA
PRIMER_INTERNAL_1_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA
PRIMER_LEFT_1=126,23
PRIMER_RIGHT_1=200,20
PRIMER_INTERNAL_1=152,26
PRIMER_LEFT_1_TM=60.381
PRIMER_RIGHT_1_TM=60.488
PRIMER_INTERNAL_1_TM=58.676
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=30.769
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=3.880529
PRIMER_LEFT_2_PENALTY=3.366081
PRIMER_RIGHT_2_PENALTY=0.514448
PRIMER_INTERNAL_2_PENALTY=6.655237
PRIMER_LEFT_2_SEQUENCE=TGCAAATAACTGTATTGCCATG
PRIMER_RIGHT_2_SEQUENCE=TCTCTCTGCCTCATTCCTCC
PRIMER_INTERNAL_2_SEQUENCE=ACCCACAAATAGTTTCTCAATTTT
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=201,20
PRIMER_INTERNAL_2=158,24
PRIMER_LEFT_2_TM=58.634
PRIMER_RIGHT_2_TM=59.486
PRIMER_INTERNAL_2_TM=57.345
PRIMER_LEFT_2_GC_PERCENT=36.364
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=3.895268
PRIMER_LEFT_3_PENALTY=3.380820
PRIMER_RIGHT_3_PENALTY=0.514448
PRIMER_INTERNAL_3_PENALTY=6.655237
PRIMER_LEFT_3_SEQUENCE=TGCAAATAACTGTATTGCCATGA
PRIMER_RIGHT_3_SEQUENCE=TCTCTCTGCCTCATTCCTCC
PRIMER_INTERNAL_3_SEQUENCE=ACCCACAAATAGTTTCTCAATTTT
PRIMER_LEFT_3=126,23
PRIMER_RIGHT_3=201,20
PRIMER_INTERNAL_3=158,24
PRIMER_LEFT_3_TM=60.381
PRIMER_RIGHT_3_TM=59.486
PRIMER_INTERNAL_3_TM=57.345
PRIMER_LEFT_3_GC_PERCENT=34.783
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=4.536196
PRIMER_LEFT_4_PENALTY=4.048260
PRIMER_RIGHT_4_PENALTY=0.487936
PRIMER_INTERNAL_4_PENALTY=7.324192
PRIMER_LEFT_4_SEQUENCE=ATGCAAATAACTGTATTGCCATG
PRIMER_RIGHT_4_SEQUENCE=CTCTCTGCCTCATTCCTCCA
PRIMER_INTERNAL_4_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA
PRIMER_LEFT_4=125,23
PRIMER_RIGHT_4=200,20
PRIMER_INTERNAL_4=152,26
PRIMER_LEFT_4_TM=58.952
PRIMER_RIGHT_4_TM=60.488
PRIMER_INTERNAL_4_TM=58.676
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=30.769
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH644
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTGGTNAAACACTGTCTAGATATTGTTGTGAAGGCATTTTGTAGATGTAATTAACATGTTCAATTAGTTGACTTGAAGAAAAGGAGATAGTCTTTGTGGGTGAACCTCATCCAATCAGTNTAAGGTCTTCAGAGANAAAACTGCAGTTTCTCTGAGAAGAAGGACTTCTATCTTACGACTATAATATGGAAGAACTGCTTGAATTTCCATCTTGATGACCTGTCCTATGGATTTCAGACTTGTTAACCCCTGCAATCATCTGACCCAATGATACAGGAGTTAAAAAGAAATTATTTAGGCAGAGAGT
SEQUENCE_INCLUDED_REGION=35,311
P3_COMMENT=CL is AAACACTGTCTAGATATTGTTGTGA
P3_COMMENT=CR is CTCTCTGCCTAAATAATTTCTTTT
P3_COMMENT=CM is AACATGTTCAATTAGTTGACTTGAA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.555001
PRIMER_LEFT_0_PENALTY=2.074231
PRIMER_RIGHT_0_PENALTY=0.480770
PRIMER_INTERNAL_0_PENALTY=3.129645
PRIMER_LEFT_0_SEQUENCE=TGCTTGAATTTCCATCTTGATG
PRIMER_RIGHT_0_SEQUENCE=CATTGGGTCAGATGATTGCA
PRIMER_INTERNAL_0_SEQUENCE=GGATTTCAGACTTGTTAACCCC
PRIMER_LEFT_0=234,22
PRIMER_RIGHT_0=308,20
PRIMER_INTERNAL_0=267,22
PRIMER_LEFT_0_TM=60.074
PRIMER_RIGHT_0_TM=60.481
PRIMER_INTERNAL_0_TM=58.870
PRIMER_LEFT_0_GC_PERCENT=36.364
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=2.618211
PRIMER_LEFT_1_PENALTY=2.074231
PRIMER_RIGHT_1_PENALTY=0.543980
PRIMER_INTERNAL_1_PENALTY=3.129645
PRIMER_LEFT_1_SEQUENCE=TGCTTGAATTTCCATCTTGATG
PRIMER_RIGHT_1_SEQUENCE=TCATTGGGTCAGATGATTGC
PRIMER_INTERNAL_1_SEQUENCE=GGATTTCAGACTTGTTAACCCC
PRIMER_LEFT_1=234,22
PRIMER_RIGHT_1=309,20
PRIMER_INTERNAL_1=267,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.456
PRIMER_INTERNAL_1_TM=58.870
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=2.658942
PRIMER_LEFT_2_PENALTY=2.592680
PRIMER_RIGHT_2_PENALTY=0.066262
PRIMER_INTERNAL_2_PENALTY=2.449829
PRIMER_LEFT_2_SEQUENCE=GGAAGAACTGCTTGAATTTCCA
PRIMER_RIGHT_2_SEQUENCE=GTCAGATGATTGCAGGGGTT
PRIMER_INTERNAL_2_SEQUENCE=TCTTGATGACCTGTCCTATGGA
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=302,20
PRIMER_INTERNAL_2=248,22
PRIMER_LEFT_2_TM=60.593
PRIMER_RIGHT_2_TM=59.934
PRIMER_INTERNAL_2_TM=59.550
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=2.658942
PRIMER_LEFT_3_PENALTY=2.592680
PRIMER_RIGHT_3_PENALTY=0.066262
PRIMER_INTERNAL_3_PENALTY=2.449829
PRIMER_LEFT_3_SEQUENCE=TGGAAGAACTGCTTGAATTTCC
PRIMER_RIGHT_3_SEQUENCE=GTCAGATGATTGCAGGGGTT
PRIMER_INTERNAL_3_SEQUENCE=TCTTGATGACCTGTCCTATGGA
PRIMER_LEFT_3=225,22
PRIMER_RIGHT_3=302,20
PRIMER_INTERNAL_3=248,22
PRIMER_LEFT_3_TM=60.593
PRIMER_RIGHT_3_TM=59.934
PRIMER_INTERNAL_3_TM=59.550
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=3.073450
PRIMER_LEFT_4_PENALTY=2.592680
PRIMER_RIGHT_4_PENALTY=0.480770
PRIMER_INTERNAL_4_PENALTY=2.449829
PRIMER_LEFT_4_SEQUENCE=GGAAGAACTGCTTGAATTTCCA
PRIMER_RIGHT_4_SEQUENCE=CATTGGGTCAGATGATTGCA
PRIMER_INTERNAL_4_SEQUENCE=TCTTGATGACCTGTCCTATGGA
PRIMER_LEFT_4=226,22
PRIMER_RIGHT_4=308,20
PRIMER_INTERNAL_4=248,22
PRIMER_LEFT_4_TM=60.593
PRIMER_RIGHT_4_TM=60.481
PRIMER_INTERNAL_4_TM=59.550
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=83
=
SEQUENCE_ID=MH646
SEQUENCE_TEMPLATE=GCTNGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGGAAGGAAGGACTGTGTTGTGTTCTCCGTTGCGTCCCCACCACGTAACACAGCAGCACTTAGCAGGTGCTTGGTCTGTATTTATTNAAAAATGNATNNATGNATATGTTGAATTAAATGNAAGTNAATTAAGTGAAAAGGATGAAACAGATGGAAGCTTCATGTTTCTTCCAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTNTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
SEQUENCE_INCLUDED_REGION=36,179
P3_COMMENT=CL is CTGTGTTGTGTTCTCCGTTG
P3_COMMENT=CR is TGAAGCTTCCATCTGTTTCA
P3_COMMENT=CM is GTCCCCACCACGTAACACAG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.423963
PRIMER_LEFT_0_PENALTY=0.229181
PRIMER_RIGHT_0_PENALTY=0.194782
PRIMER_INTERNAL_0_PENALTY=1.277790
PRIMER_LEFT_0_SEQUENCE=TCCTGGAAGGAAGGACTGTG
PRIMER_RIGHT_0_SEQUENCE=CAAGCACCTGCTAAGTGCTG
PRIMER_INTERNAL_0_SEQUENCE=GTCCCCACCACGTAACACA
PRIMER_LEFT_0=37,20
PRIMER_RIGHT_0=112,20
PRIMER_INTERNAL_0=73,19
PRIMER_LEFT_0_TM=60.229
PRIMER_RIGHT_0_TM=59.805
PRIMER_INTERNAL_0_TM=60.278
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=0.691018
PRIMER_LEFT_1_PENALTY=0.229181
PRIMER_RIGHT_1_PENALTY=0.461837
PRIMER_INTERNAL_1_PENALTY=1.277790
PRIMER_LEFT_1_SEQUENCE=TCCTGGAAGGAAGGACTGTG
PRIMER_RIGHT_1_SEQUENCE=ACCAAGCACCTGCTAAGTGC
PRIMER_INTERNAL_1_SEQUENCE=GTCCCCACCACGTAACACA
PRIMER_LEFT_1=37,20
PRIMER_RIGHT_1=114,20
PRIMER_INTERNAL_1=73,19
PRIMER_LEFT_1_TM=60.229
PRIMER_RIGHT_1_TM=60.462
PRIMER_INTERNAL_1_TM=60.278
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=0.821413
PRIMER_LEFT_2_PENALTY=0.229181
PRIMER_RIGHT_2_PENALTY=0.592232
PRIMER_INTERNAL_2_PENALTY=1.277790
PRIMER_LEFT_2_SEQUENCE=TCCTGGAAGGAAGGACTGTG
PRIMER_RIGHT_2_SEQUENCE=CCAAGCACCTGCTAAGTGCT
PRIMER_INTERNAL_2_SEQUENCE=GTCCCCACCACGTAACACA
PRIMER_LEFT_2=37,20
PRIMER_RIGHT_2=113,20
PRIMER_INTERNAL_2=73,19
PRIMER_LEFT_2_TM=60.229
PRIMER_RIGHT_2_TM=60.592
PRIMER_INTERNAL_2_TM=60.278
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=8.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=0.969455
PRIMER_LEFT_3_PENALTY=0.229181
PRIMER_RIGHT_3_PENALTY=0.740274
PRIMER_INTERNAL_3_PENALTY=1.277790
PRIMER_LEFT_3_SEQUENCE=TCCTGGAAGGAAGGACTGTG
PRIMER_RIGHT_3_SEQUENCE=AAGCACCTGCTAAGTGCTGC
PRIMER_INTERNAL_3_SEQUENCE=GTCCCCACCACGTAACACA
PRIMER_LEFT_3=37,20
PRIMER_RIGHT_3=111,20
PRIMER_INTERNAL_3=73,19
PRIMER_LEFT_3_TM=60.229
PRIMER_RIGHT_3_TM=60.740
PRIMER_INTERNAL_3_TM=60.278
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=1.045598
PRIMER_LEFT_4_PENALTY=0.850816
PRIMER_RIGHT_4_PENALTY=0.194782
PRIMER_INTERNAL_4_PENALTY=1.277790
PRIMER_LEFT_4_SEQUENCE=CCTGGAAGGAAGGACTGTGT
PRIMER_RIGHT_4_SEQUENCE=CAAGCACCTGCTAAGTGCTG
PRIMER_INTERNAL_4_SEQUENCE=GTCCCCACCACGTAACACA
PRIMER_LEFT_4=38,20
PRIMER_RIGHT_4=112,20
PRIMER_INTERNAL_4=73,19
PRIMER_LEFT_4_TM=59.149
PRIMER_RIGHT_4_TM=59.805
PRIMER_INTERNAL_4_TM=60.278
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH647
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTTGCCATATTATTAAAAGATATCCATCCTACTACTTTTTTACTATTATTTCACTTAACCAGATTGGAAAGAAATTTTATTTGATATCCGCAGCTCTAACCTAGCAATGGAATTTGAGTAATATGTTCCCTACAGCCATATTTTAATATGTAAAAAAGCTTATATCCCTGAAGTTCAATATTATGACACTATACACTATGTTATGACATATCTATTTTCTTAGAATGAAGTGANGAAAGGGAAGACTATGGTATTCTCAGAACTTGGGACTCAAACTCATATTATTGCAGGG
SEQUENCE_INCLUDED_REGION=36,295
P3_COMMENT=CL is TGATATCCGCAGCTCTAACC
P3_COMMENT=CR is CTGCAATAATATGAGTTTGAGTCC
P3_COMMENT=CM is AGCAATGGAATTTGAGTAATATGTT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.808663
PRIMER_LEFT_0_PENALTY=0.668546
PRIMER_RIGHT_0_PENALTY=6.140118
PRIMER_INTERNAL_0_PENALTY=4.081412
PRIMER_LEFT_0_SEQUENCE=CCGCAGCTCTAACCTAGCAA
PRIMER_RIGHT_0_SEQUENCE=TGAACTTCAGGGATATAAGCTTTTT
PRIMER_INTERNAL_0_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
PRIMER_LEFT_0=126,20
PRIMER_RIGHT_0=215,25
PRIMER_INTERNAL_0=153,24
PRIMER_LEFT_0_TM=60.669
PRIMER_RIGHT_0_TM=58.860
PRIMER_INTERNAL_0_TM=59.919
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=32.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=6.808663
PRIMER_LEFT_1_PENALTY=0.668546
PRIMER_RIGHT_1_PENALTY=6.140118
PRIMER_INTERNAL_1_PENALTY=4.081412
PRIMER_LEFT_1_SEQUENCE=CCGCAGCTCTAACCTAGCAA
PRIMER_RIGHT_1_SEQUENCE=TTGAACTTCAGGGATATAAGCTTTT
PRIMER_INTERNAL_1_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
PRIMER_LEFT_1=126,20
PRIMER_RIGHT_1=216,25
PRIMER_INTERNAL_1=153,24
PRIMER_LEFT_1_TM=60.669
PRIMER_RIGHT_1_TM=58.860
PRIMER_INTERNAL_1_TM=59.919
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=32.000
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=6.990026
PRIMER_LEFT_2_PENALTY=0.668546
PRIMER_RIGHT_2_PENALTY=6.321480
PRIMER_INTERNAL_2_PENALTY=4.081412
PRIMER_LEFT_2_SEQUENCE=CCGCAGCTCTAACCTAGCAA
PRIMER_RIGHT_2_SEQUENCE=TGAACTTCAGGGATATAAGCTTTT
PRIMER_INTERNAL_2_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
PRIMER_LEFT_2=126,20
PRIMER_RIGHT_2=215,24
PRIMER_INTERNAL_2=153,24
PRIMER_LEFT_2_TM=60.669
PRIMER_RIGHT_2_TM=57.679
PRIMER_INTERNAL_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=33.333
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=6.990026
PRIMER_LEFT_3_PENALTY=0.668546
PRIMER_RIGHT_3_PENALTY=6.321480
PRIMER_INTERNAL_3_PENALTY=4.081412
PRIMER_LEFT_3_SEQUENCE=CCGCAGCTCTAACCTAGCAA
PRIMER_RIGHT_3_SEQUENCE=TTGAACTTCAGGGATATAAGCTTT
PRIMER_INTERNAL_3_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
PRIMER_LEFT_3=126,20
PRIMER_RIGHT_3=216,24
PRIMER_INTERNAL_3=153,24
PRIMER_LEFT_3_TM=60.669
PRIMER_RIGHT_3_TM=57.679
PRIMER_INTERNAL_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=7.319712
PRIMER_LEFT_4_PENALTY=1.179594
PRIMER_RIGHT_4_PENALTY=6.140118
PRIMER_INTERNAL_4_PENALTY=4.081412
PRIMER_LEFT_4_SEQUENCE=CGCAGCTCTAACCTAGCAATG
PRIMER_RIGHT_4_SEQUENCE=TGAACTTCAGGGATATAAGCTTTTT
PRIMER_INTERNAL_4_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA
PRIMER_LEFT_4=127,21
PRIMER_RIGHT_4=215,25
PRIMER_INTERNAL_4=153,24
PRIMER_LEFT_4_TM=60.180
PRIMER_RIGHT_4_TM=58.860
PRIMER_INTERNAL_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=32.000
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH648
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCAGTNATTTCAAGTGATTGCTGTGGGCATTCTATTTTAGGTNCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAGAGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTAGGAAACATTATTCCCCTTTTTTAATTGCTTTGTTATTTGCTCGTTTAGTTGGATTTTGTGTACTTATTTGCAAATACATATTAGTTAGTGGCTTTTTGTCTTTTAAGAGAAGTTCTGCTCTACTTATGTAGGCAGTTTTCAACTCAGTTATCTCTTGAATTGAAAATAGGAGTAAAGGAAATTAAAATTTTCT
SEQUENCE_INCLUDED_REGION=36,340
P3_COMMENT=CL is TTGCTGTGGGCATTCTATTT
P3_COMMENT=CR is TCCTTTACTCCTATTTTCAATTCA
P3_COMMENT=CM is CTGGTACTCAGGAATCATGGTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.586707
PRIMER_LEFT_0_PENALTY=0.308722
PRIMER_RIGHT_0_PENALTY=0.277985
PRIMER_INTERNAL_0_PENALTY=2.527462
PRIMER_LEFT_0_SEQUENCE=TTTCAAGTGATTGCTGTGGG
PRIMER_RIGHT_0_SEQUENCE=TGTTTCTCCAAGTGCAACCA
PRIMER_INTERNAL_0_SEQUENCE=CTGGTACTCAGGAATCATGGTG
PRIMER_LEFT_0=48,20
PRIMER_RIGHT_0=125,20
PRIMER_INTERNAL_0=84,22
PRIMER_LEFT_0_TM=59.691
PRIMER_RIGHT_0_TM=60.278
PRIMER_INTERNAL_0_TM=59.473
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=1.292603
PRIMER_LEFT_1_PENALTY=1.014618
PRIMER_RIGHT_1_PENALTY=0.277985
PRIMER_INTERNAL_1_PENALTY=2.527462
PRIMER_LEFT_1_SEQUENCE=ATTTCAAGTGATTGCTGTGGG
PRIMER_RIGHT_1_SEQUENCE=TGTTTCTCCAAGTGCAACCA
PRIMER_INTERNAL_1_SEQUENCE=CTGGTACTCAGGAATCATGGTG
PRIMER_LEFT_1=47,21
PRIMER_RIGHT_1=125,20
PRIMER_INTERNAL_1=84,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=60.278
PRIMER_INTERNAL_1_TM=59.473
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=1.852829
PRIMER_LEFT_2_PENALTY=0.308722
PRIMER_RIGHT_2_PENALTY=1.544107
PRIMER_INTERNAL_2_PENALTY=2.527462
PRIMER_LEFT_2_SEQUENCE=TTTCAAGTGATTGCTGTGGG
PRIMER_RIGHT_2_SEQUENCE=ATGTTTCTCCAAGTGCAACCA
PRIMER_INTERNAL_2_SEQUENCE=CTGGTACTCAGGAATCATGGTG
PRIMER_LEFT_2=48,20
PRIMER_RIGHT_2=126,21
PRIMER_INTERNAL_2=84,22
PRIMER_LEFT_2_TM=59.691
PRIMER_RIGHT_2_TM=60.544
PRIMER_INTERNAL_2_TM=59.473
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=1.882221
PRIMER_LEFT_3_PENALTY=0.180577
PRIMER_RIGHT_3_PENALTY=1.701644
PRIMER_INTERNAL_3_PENALTY=2.274705
PRIMER_LEFT_3_SEQUENCE=GAATCATGGTGTGGTTGCAC
PRIMER_RIGHT_3_SEQUENCE=TCCTACCTGAATCTCACAGGC
PRIMER_INTERNAL_3_SEQUENCE=TTGGAGAAACATTAGAGGAGGC
PRIMER_LEFT_3=95,20
PRIMER_RIGHT_3=185,21
PRIMER_INTERNAL_3=115,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=59.298
PRIMER_INTERNAL_3_TM=59.725
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=1.896048
PRIMER_LEFT_4_PENALTY=0.194404
PRIMER_RIGHT_4_PENALTY=1.701644
PRIMER_INTERNAL_4_PENALTY=2.274705
PRIMER_LEFT_4_SEQUENCE=CAGGAATCATGGTGTGGTTG
PRIMER_RIGHT_4_SEQUENCE=TCCTACCTGAATCTCACAGGC
PRIMER_INTERNAL_4_SEQUENCE=TTGGAGAAACATTAGAGGAGGC
PRIMER_LEFT_4=92,20
PRIMER_RIGHT_4=185,21
PRIMER_INTERNAL_4=115,22
PRIMER_LEFT_4_TM=59.806
PRIMER_RIGHT_4_TM=59.298
PRIMER_INTERNAL_4_TM=59.725
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=94
=
SEQUENCE_ID=MH650
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAACAGACCTGTGCCAAGGTTCACCTAACCTAGTACCTAACCTCATGTTTGTTTTACATGCATAAGACAATTCTGGGAAGATTAAAGTGTAAATTCCTAGTGGAGAATTTGAGTGGCCATTTTAATTATTTAATTAATTAATTTTGGTCCAGCACTCTTGTCTGATATTTTCTTCAGGTGACAGACTACACTTTTGAGAAACAATGGTGTTTTTCTTGTGCANCTCTGACTTGAATATACCAATCTGAACCGCTGCCAGTGGAGAATCTAACCACCTTCAAAATGATATCACTTTCATTCAATAATAAACAT
SEQUENCE_INCLUDED_REGION=36,314
P3_COMMENT=CL is CTCAACAGACCTGTGCCAAG
P3_COMMENT=CR is ATGAAAGTGATATCATTTTGAAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.404345
PRIMER_LEFT_0_PENALTY=0.022009
PRIMER_RIGHT_0_PENALTY=1.382337
PRIMER_INTERNAL_0_PENALTY=5.042469
PRIMER_LEFT_0_SEQUENCE=TGGTCCAGCACTCTTGTCTG
PRIMER_RIGHT_0_SEQUENCE=TGCACAAGAAAAACACCATTG
PRIMER_INTERNAL_0_SEQUENCE=TCAGGTGACAGACTACACTTTTGA
PRIMER_LEFT_0=182,20
PRIMER_RIGHT_0=259,21
PRIMER_INTERNAL_0=211,24
PRIMER_LEFT_0_TM=60.022
PRIMER_RIGHT_0_TM=59.618
PRIMER_INTERNAL_0_TM=58.958
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=2.198869
PRIMER_LEFT_1_PENALTY=0.158723
PRIMER_RIGHT_1_PENALTY=2.040146
PRIMER_INTERNAL_1_PENALTY=7.587579
PRIMER_LEFT_1_SEQUENCE=ACAGACCTGTGCCAAGGTTC
PRIMER_RIGHT_1_SEQUENCE=TCCCAGAATTGTCTTATGCATG
PRIMER_INTERNAL_1_SEQUENCE=CCTAGTACCTAACCTCATGTTTGTTTT
PRIMER_LEFT_1=40,20
PRIMER_RIGHT_1=115,22
PRIMER_INTERNAL_1=66,27
PRIMER_LEFT_1_TM=60.159
PRIMER_RIGHT_1_TM=59.960
PRIMER_INTERNAL_1_TM=59.412
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_INTERNAL_1_GC_PERCENT=37.037
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=2.414734
PRIMER_LEFT_2_PENALTY=1.032397
PRIMER_RIGHT_2_PENALTY=1.382337
PRIMER_INTERNAL_2_PENALTY=5.042469
PRIMER_LEFT_2_SEQUENCE=GGTCCAGCACTCTTGTCTGA
PRIMER_RIGHT_2_SEQUENCE=TGCACAAGAAAAACACCATTG
PRIMER_INTERNAL_2_SEQUENCE=TCAGGTGACAGACTACACTTTTGA
PRIMER_LEFT_2=183,20
PRIMER_RIGHT_2=259,21
PRIMER_INTERNAL_2=211,24
PRIMER_LEFT_2_TM=58.968
PRIMER_RIGHT_2_TM=59.618
PRIMER_INTERNAL_2_TM=58.958
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=2.417978
PRIMER_LEFT_3_PENALTY=0.377832
PRIMER_RIGHT_3_PENALTY=2.040146
PRIMER_INTERNAL_3_PENALTY=7.284315
PRIMER_LEFT_3_SEQUENCE=AACAGACCTGTGCCAAGGTT
PRIMER_RIGHT_3_SEQUENCE=TCCCAGAATTGTCTTATGCATG
PRIMER_INTERNAL_3_SEQUENCE=CACCTAACCTAGTACCTAACCTCATG
PRIMER_LEFT_3=39,20
PRIMER_RIGHT_3=115,22
PRIMER_INTERNAL_3=59,26
PRIMER_LEFT_3_TM=59.622
PRIMER_RIGHT_3_TM=59.960
PRIMER_INTERNAL_3_TM=58.716
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_INTERNAL_3_GC_PERCENT=46.154
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=2.462481
PRIMER_LEFT_4_PENALTY=0.022009
PRIMER_RIGHT_4_PENALTY=2.440473
PRIMER_INTERNAL_4_PENALTY=5.042469
PRIMER_LEFT_4_SEQUENCE=TGGTCCAGCACTCTTGTCTG
PRIMER_RIGHT_4_SEQUENCE=TGCACAAGAAAAACACCATTGT
PRIMER_INTERNAL_4_SEQUENCE=TCAGGTGACAGACTACACTTTTGA
PRIMER_LEFT_4=182,20
PRIMER_RIGHT_4=259,22
PRIMER_INTERNAL_4=211,24
PRIMER_LEFT_4_TM=60.022
PRIMER_RIGHT_4_TM=60.440
PRIMER_INTERNAL_4_TM=58.958
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=36.364
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH652
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGGTNACAAGTNGAGGAAAGCAGGAAGGGAGTGAGAAGCCTGAAGCCTCTAGCCACCAGTTTCCCCAGGCAGTCCCCCATCCAAGCAACAGTCAGGCCTGACCTTGCTTAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
SEQUENCE_INCLUDED_REGION=36,111
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.480700
PRIMER_LEFT_0_PENALTY=0.182682
PRIMER_RIGHT_0_PENALTY=0.298018
PRIMER_INTERNAL_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=GAGGAAAGCAGGAAGGGAGT
PRIMER_RIGHT_0_SEQUENCE=AAGGTCAGGCCTGACTGTTG
PRIMER_INTERNAL_0_SEQUENCE=TGAAGCCTCTAGCCACCAGT
PRIMER_LEFT_0=49,20
PRIMER_RIGHT_0=141,20
PRIMER_INTERNAL_0=77,20
PRIMER_LEFT_0_TM=59.817
PRIMER_RIGHT_0_TM=60.298
PRIMER_INTERNAL_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=12.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=93
PRIMER_PAIR_1_PENALTY=0.636014
PRIMER_LEFT_1_PENALTY=0.182682
PRIMER_RIGHT_1_PENALTY=0.453332
PRIMER_INTERNAL_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=GAGGAAAGCAGGAAGGGAGT
PRIMER_RIGHT_1_SEQUENCE=GTCAGGCCTGACTGTTGCTT
PRIMER_INTERNAL_1_SEQUENCE=TGAAGCCTCTAGCCACCAGT
PRIMER_LEFT_1=49,20
PRIMER_RIGHT_1=138,20
PRIMER_INTERNAL_1=77,20
PRIMER_LEFT_1_TM=59.817
PRIMER_RIGHT_1_TM=60.453
PRIMER_INTERNAL_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=12.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=0.768018
PRIMER_LEFT_2_PENALTY=0.470001
PRIMER_RIGHT_2_PENALTY=0.298018
PRIMER_INTERNAL_2_PENALTY=1.007677
PRIMER_LEFT_2_SEQUENCE=GAAGGGAGTGAGAAGCCTGA
PRIMER_RIGHT_2_SEQUENCE=AAGGTCAGGCCTGACTGTTG
PRIMER_INTERNAL_2_SEQUENCE=CTCTAGCCACCAGTTTCCCC
PRIMER_LEFT_2=60,20
PRIMER_RIGHT_2=141,20
PRIMER_INTERNAL_2=83,20
PRIMER_LEFT_2_TM=59.530
PRIMER_RIGHT_2_TM=60.298
PRIMER_INTERNAL_2_TM=61.008
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=12.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=0.859125
PRIMER_LEFT_3_PENALTY=0.561107
PRIMER_RIGHT_3_PENALTY=0.298018
PRIMER_INTERNAL_3_PENALTY=1.007677
PRIMER_LEFT_3_SEQUENCE=AGGAAGGGAGTGAGAAGCCT
PRIMER_RIGHT_3_SEQUENCE=AAGGTCAGGCCTGACTGTTG
PRIMER_INTERNAL_3_SEQUENCE=CTCTAGCCACCAGTTTCCCC
PRIMER_LEFT_3=58,20
PRIMER_RIGHT_3=141,20
PRIMER_INTERNAL_3=83,20
PRIMER_LEFT_3_TM=59.439
PRIMER_RIGHT_3_TM=60.298
PRIMER_INTERNAL_3_TM=61.008
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=12.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=0.923333
PRIMER_LEFT_4_PENALTY=0.470001
PRIMER_RIGHT_4_PENALTY=0.453332
PRIMER_INTERNAL_4_PENALTY=1.007677
PRIMER_LEFT_4_SEQUENCE=GAAGGGAGTGAGAAGCCTGA
PRIMER_RIGHT_4_SEQUENCE=GTCAGGCCTGACTGTTGCTT
PRIMER_INTERNAL_4_SEQUENCE=CTCTAGCCACCAGTTTCCCC
PRIMER_LEFT_4=60,20
PRIMER_RIGHT_4=138,20
PRIMER_INTERNAL_4=83,20
PRIMER_LEFT_4_TM=59.530
PRIMER_RIGHT_4_TM=60.453
PRIMER_INTERNAL_4_TM=61.008
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=12.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH657
SEQUENCE_TEMPLATE=ANGNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAACATGACCACCCTCCCCACCATTGCTGCTGGGATGAGCACCCAGACCAGGGCACCAGAAAGCCACGGAAGCCACAGCCACTAGCCCTGAACCTTGCACACCCCTGGAGTTTCTCTCCCCTCCCTATCCCCTCACCAACACCTTCCAGTGCTTATTCTGCTGTGTCAAAATGATCCTTCTGTTGCTGCACTGTCATTACTGTTGTATGGATTTATAATTATTGTCCAAAAAAGCCCCGAGCCTGGTACAGAAAAGGCATGGGGAAAGATGTGTCAGAATGCACAGTG
SEQUENCE_INCLUDED_REGION=38,290
P3_COMMENT=CL is GACCAGGGCACCAGAAAG
P3_COMMENT=CR is TCTGACACATCTTTCCCCAT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.075898
PRIMER_LEFT_0_PENALTY=0.008461
PRIMER_RIGHT_0_PENALTY=0.067436
PRIMER_INTERNAL_0_PENALTY=1.080628
PRIMER_LEFT_0_SEQUENCE=TGATCCTTCTGTTGCTGCAC
PRIMER_RIGHT_0_SEQUENCE=TCCCCATGCCTTTTCTGTAC
PRIMER_INTERNAL_0_SEQUENCE=TTATTGTCCAAAAAAGCCCC
PRIMER_LEFT_0=211,20
PRIMER_RIGHT_0=304,20
PRIMER_INTERNAL_0=258,20
PRIMER_LEFT_0_TM=59.992
PRIMER_RIGHT_0_TM=59.933
PRIMER_INTERNAL_0_TM=58.919
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=5.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=0.133117
PRIMER_LEFT_1_PENALTY=0.008461
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_INTERNAL_1_PENALTY=1.080628
PRIMER_LEFT_1_SEQUENCE=TGATCCTTCTGTTGCTGCAC
PRIMER_RIGHT_1_SEQUENCE=TGCCTTTTCTGTACCAGGCT
PRIMER_INTERNAL_1_SEQUENCE=TTATTGTCCAAAAAAGCCCC
PRIMER_LEFT_1=211,20
PRIMER_RIGHT_1=298,20
PRIMER_INTERNAL_1=258,20
PRIMER_LEFT_1_TM=59.992
PRIMER_RIGHT_1_TM=59.875
PRIMER_INTERNAL_1_TM=58.919
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=0.139601
PRIMER_LEFT_2_PENALTY=0.008461
PRIMER_RIGHT_2_PENALTY=0.131140
PRIMER_INTERNAL_2_PENALTY=7.859678
PRIMER_LEFT_2_SEQUENCE=TGATCCTTCTGTTGCTGCAC
PRIMER_RIGHT_2_SEQUENCE=CTTTTCTGTACCAGGCTCGG
PRIMER_INTERNAL_2_SEQUENCE=TTTATAATTATTGTCCAAAAAAGCC
PRIMER_LEFT_2=211,20
PRIMER_RIGHT_2=295,20
PRIMER_INTERNAL_2=251,25
PRIMER_LEFT_2_TM=59.992
PRIMER_RIGHT_2_TM=59.869
PRIMER_INTERNAL_2_TM=57.140
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=24.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=0.139601
PRIMER_LEFT_3_PENALTY=0.008461
PRIMER_RIGHT_3_PENALTY=0.131140
PRIMER_INTERNAL_3_PENALTY=3.789337
PRIMER_LEFT_3_SEQUENCE=TGATCCTTCTGTTGCTGCAC
PRIMER_RIGHT_3_SEQUENCE=CCTTTTCTGTACCAGGCTCG
PRIMER_INTERNAL_3_SEQUENCE=AATTATTGTCCAAAAAAGCCC
PRIMER_LEFT_3=211,20
PRIMER_RIGHT_3=296,20
PRIMER_INTERNAL_3=256,21
PRIMER_LEFT_3_TM=59.992
PRIMER_RIGHT_3_TM=59.869
PRIMER_INTERNAL_3_TM=57.211
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=0.194404
PRIMER_LEFT_4_PENALTY=0.185942
PRIMER_RIGHT_4_PENALTY=0.008461
PRIMER_INTERNAL_4_PENALTY=0.001978
PRIMER_LEFT_4_SEQUENCE=CTGGAGTTTCTCTCCCCTCC
PRIMER_RIGHT_4_SEQUENCE=GTGCAGCAACAGAAGGATCA
PRIMER_INTERNAL_4_SEQUENCE=CCTCACCAACACCTTCCAGT
PRIMER_LEFT_4=144,20
PRIMER_RIGHT_4=230,20
PRIMER_INTERNAL_4=170,20
PRIMER_LEFT_4_TM=60.186
PRIMER_RIGHT_4_TM=59.992
PRIMER_INTERNAL_4_TM=60.002
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH660
SEQUENCE_TEMPLATE=GCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCCTGGCCGCCCACCCCGCCCCGCTCCCTGCGAGGAGCGNAGNATCTCAGAAACCAGAAACGGCGCCCACCGCACCCCACACGGCCCCACCTGGGGCCCNTCCACGTGNATGTCCTCGCGGAACTGGGCCAGCGGGTAGACAAGTCGCAGAAGAGCGAGGAGTCCGCGGCGGGGAAGGCGGCGTCCCGGAACAG
SEQUENCE_INCLUDED_REGION=32,197
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.198301
PRIMER_LEFT_0_PENALTY=2.381087
PRIMER_RIGHT_0_PENALTY=1.817214
PRIMER_INTERNAL_0_PENALTY=4.236162
PRIMER_LEFT_0_SEQUENCE=CAGAAACCAGAAACGGCG
PRIMER_RIGHT_0_SEQUENCE=GACTTGTCTACCCGCTGGC
PRIMER_INTERNAL_0_SEQUENCE=ATGTCCTCGCGGAACTGG
PRIMER_LEFT_0=82,18
PRIMER_RIGHT_0=180,19
PRIMER_INTERNAL_0=144,18
PRIMER_LEFT_0_TM=60.381
PRIMER_RIGHT_0_TM=60.817
PRIMER_INTERNAL_0_TM=62.236
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_INTERNAL_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=13.4000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=5.112432
PRIMER_LEFT_1_PENALTY=3.295218
PRIMER_RIGHT_1_PENALTY=1.817214
PRIMER_INTERNAL_1_PENALTY=4.236162
PRIMER_LEFT_1_SEQUENCE=TCAGAAACCAGAAACGGCG
PRIMER_RIGHT_1_SEQUENCE=GACTTGTCTACCCGCTGGC
PRIMER_INTERNAL_1_SEQUENCE=ATGTCCTCGCGGAACTGG
PRIMER_LEFT_1=81,19
PRIMER_RIGHT_1=180,19
PRIMER_INTERNAL_1=144,18
PRIMER_LEFT_1_TM=62.295
PRIMER_RIGHT_1_TM=60.817
PRIMER_INTERNAL_1_TM=62.236
PRIMER_LEFT_1_GC_PERCENT=52.632
PRIMER_RIGHT_1_GC_PERCENT=63.158
PRIMER_INTERNAL_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=13.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=5.146012
PRIMER_LEFT_2_PENALTY=3.328798
PRIMER_RIGHT_2_PENALTY=1.817214
PRIMER_INTERNAL_2_PENALTY=4.236162
PRIMER_LEFT_2_SEQUENCE=AGAAACCAGAAACGGCGC
PRIMER_RIGHT_2_SEQUENCE=GACTTGTCTACCCGCTGGC
PRIMER_INTERNAL_2_SEQUENCE=ATGTCCTCGCGGAACTGG
PRIMER_LEFT_2=83,18
PRIMER_RIGHT_2=180,19
PRIMER_INTERNAL_2=144,18
PRIMER_LEFT_2_TM=61.329
PRIMER_RIGHT_2_TM=60.817
PRIMER_INTERNAL_2_TM=62.236
PRIMER_LEFT_2_GC_PERCENT=55.556
PRIMER_RIGHT_2_GC_PERCENT=63.158
PRIMER_INTERNAL_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=98
PRIMER_PAIR_3_PENALTY=5.178689
PRIMER_LEFT_3_PENALTY=2.381087
PRIMER_RIGHT_3_PENALTY=2.797602
PRIMER_INTERNAL_3_PENALTY=4.236162
PRIMER_LEFT_3_SEQUENCE=CAGAAACCAGAAACGGCG
PRIMER_RIGHT_3_SEQUENCE=CGACTTGTCTACCCGCTGG
PRIMER_INTERNAL_3_SEQUENCE=ATGTCCTCGCGGAACTGG
PRIMER_LEFT_3=82,18
PRIMER_RIGHT_3=181,19
PRIMER_INTERNAL_3=144,18
PRIMER_LEFT_3_TM=60.381
PRIMER_RIGHT_3_TM=61.798
PRIMER_INTERNAL_3_TM=62.236
PRIMER_LEFT_3_GC_PERCENT=55.556
PRIMER_RIGHT_3_GC_PERCENT=63.158
PRIMER_INTERNAL_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=13.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=5.551363
PRIMER_LEFT_4_PENALTY=2.381087
PRIMER_RIGHT_4_PENALTY=3.170275
PRIMER_INTERNAL_4_PENALTY=4.236162
PRIMER_LEFT_4_SEQUENCE=CAGAAACCAGAAACGGCG
PRIMER_RIGHT_4_SEQUENCE=ACTTGTCTACCCGCTGGC
PRIMER_INTERNAL_4_SEQUENCE=ATGTCCTCGCGGAACTGG
PRIMER_LEFT_4=82,18
PRIMER_RIGHT_4=179,18
PRIMER_INTERNAL_4=144,18
PRIMER_LEFT_4_TM=60.381
PRIMER_RIGHT_4_TM=58.830
PRIMER_INTERNAL_4_TM=62.236
PRIMER_LEFT_4_GC_PERCENT=55.556
PRIMER_RIGHT_4_GC_PERCENT=61.111
PRIMER_INTERNAL_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=13.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH661
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCCTCCCGGTGCCGCCCGCGCCTGCTCCTAGGTTCTTTTTAAGAGGTGTTTTAAAGGTGTTTCACTTTATACTAGAATAACACAAAGAAAATATTAAGAAGATTTAACAAGAATAAAATCATAATTAATTACAGGCATATCAGTGTCTGAGAAGATGATGTGTAGAAAACTGATTTGAGCAGGAATAGTCTGTATTACAGTTTCCTGATATGATTTTCTCGGTAACTGGGAGAACTAGACAGAAGATAAAGGTAATAGGGTTTA
SEQUENCE_INCLUDED_REGION=36,265
P3_COMMENT=CL is GCCTGCTCCTAGGTTCTTTT
P3_COMMENT=CR is CCCAGTTACCGAGAAAATCA
P3_COMMENT=CM is AAGAGGTGTTTTAAAGGTGTTTCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.426974
PRIMER_LEFT_0_PENALTY=3.981846
PRIMER_RIGHT_0_PENALTY=1.445128
PRIMER_INTERNAL_0_PENALTY=3.876179
PRIMER_LEFT_0_SEQUENCE=CAGGCATATCAGTGTCTGAGAAG
PRIMER_RIGHT_0_SEQUENCE=TCCCAGTTACCGAGAAAATCA
PRIMER_INTERNAL_0_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
PRIMER_LEFT_0=168,23
PRIMER_RIGHT_0=267,21
PRIMER_INTERNAL_0=200,22
PRIMER_LEFT_0_TM=59.018
PRIMER_RIGHT_0_TM=59.555
PRIMER_INTERNAL_0_TM=58.124
PRIMER_LEFT_0_GC_PERCENT=47.826
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=5.465596
PRIMER_LEFT_1_PENALTY=4.020468
PRIMER_RIGHT_1_PENALTY=1.445128
PRIMER_INTERNAL_1_PENALTY=3.876179
PRIMER_LEFT_1_SEQUENCE=CAGGCATATCAGTGTCTGAGAA
PRIMER_RIGHT_1_SEQUENCE=TCCCAGTTACCGAGAAAATCA
PRIMER_INTERNAL_1_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
PRIMER_LEFT_1=168,22
PRIMER_RIGHT_1=267,21
PRIMER_INTERNAL_1=200,22
PRIMER_LEFT_1_TM=57.980
PRIMER_RIGHT_1_TM=59.555
PRIMER_INTERNAL_1_TM=58.124
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_INTERNAL_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=5.603425
PRIMER_LEFT_2_PENALTY=4.158297
PRIMER_RIGHT_2_PENALTY=1.445128
PRIMER_INTERNAL_2_PENALTY=3.876179
PRIMER_LEFT_2_SEQUENCE=GGCATATCAGTGTCTGAGAAGATG
PRIMER_RIGHT_2_SEQUENCE=TCCCAGTTACCGAGAAAATCA
PRIMER_INTERNAL_2_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
PRIMER_LEFT_2=170,24
PRIMER_RIGHT_2=267,21
PRIMER_INTERNAL_2=200,22
PRIMER_LEFT_2_TM=60.158
PRIMER_RIGHT_2_TM=59.555
PRIMER_INTERNAL_2_TM=58.124
PRIMER_LEFT_2_GC_PERCENT=45.833
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=36.364
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=98
PRIMER_PAIR_3_PENALTY=6.146485
PRIMER_LEFT_3_PENALTY=3.981846
PRIMER_RIGHT_3_PENALTY=2.164639
PRIMER_INTERNAL_3_PENALTY=3.876179
PRIMER_LEFT_3_SEQUENCE=CAGGCATATCAGTGTCTGAGAAG
PRIMER_RIGHT_3_SEQUENCE=TCCCAGTTACCGAGAAAATCAT
PRIMER_INTERNAL_3_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
PRIMER_LEFT_3=168,23
PRIMER_RIGHT_3=267,22
PRIMER_INTERNAL_3=200,22
PRIMER_LEFT_3_TM=59.018
PRIMER_RIGHT_3_TM=59.835
PRIMER_INTERNAL_3_TM=58.124
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_INTERNAL_3_GC_PERCENT=36.364
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=6.185107
PRIMER_LEFT_4_PENALTY=4.020468
PRIMER_RIGHT_4_PENALTY=2.164639
PRIMER_INTERNAL_4_PENALTY=3.876179
PRIMER_LEFT_4_SEQUENCE=CAGGCATATCAGTGTCTGAGAA
PRIMER_RIGHT_4_SEQUENCE=TCCCAGTTACCGAGAAAATCAT
PRIMER_INTERNAL_4_SEQUENCE=AGAAAACTGATTTGAGCAGGAA
PRIMER_LEFT_4=168,22
PRIMER_RIGHT_4=267,22
PRIMER_INTERNAL_4=200,22
PRIMER_LEFT_4_TM=57.980
PRIMER_RIGHT_4_TM=59.835
PRIMER_INTERNAL_4_TM=58.124
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_INTERNAL_4_GC_PERCENT=36.364
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH665
SEQUENCE_TEMPLATE=GGAACACTATGTAGAGANACACAGGTGCAATTCCTNCTNCCCTTTGACCTTATAGTGAGTGNCCCTGCATGAGGGTCATGATAATCACACCAAAAATACCTAATTTCAACTTAAGTGAGAACTGTGAAAGCCTGACCCTAACTAGGAGGCCTGAAGTGGAATTTCTGAGAAG
SEQUENCE_INCLUDED_REGION=0,172
P3_COMMENT=CL is CCCTTTGACCTTATAGTGAGTG
P3_COMMENT=CR is TCTCAGAAATTCCACTTCAGG
P3_COMMENT=CM is CCCTGCATGAGGGTCATG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.597570
PRIMER_LEFT_0_PENALTY=0.523704
PRIMER_RIGHT_0_PENALTY=0.073866
PRIMER_INTERNAL_0_PENALTY=0.296722
PRIMER_LEFT_0_SEQUENCE=CCTGCATGAGGGTCATGATA
PRIMER_RIGHT_0_SEQUENCE=AATTCCACTTCAGGCCTCCT
PRIMER_INTERNAL_0_SEQUENCE=GAACTGTGAAAGCCTGACCC
PRIMER_LEFT_0=63,20
PRIMER_RIGHT_0=162,20
PRIMER_INTERNAL_0=118,20
PRIMER_LEFT_0_TM=59.476
PRIMER_RIGHT_0_TM=60.074
PRIMER_INTERNAL_0_TM=59.703
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.820426
PRIMER_LEFT_1_PENALTY=0.523704
PRIMER_RIGHT_1_PENALTY=0.296722
PRIMER_INTERNAL_1_PENALTY=6.072226
PRIMER_LEFT_1_SEQUENCE=CCTGCATGAGGGTCATGATA
PRIMER_RIGHT_1_SEQUENCE=GGGTCAGGCTTTCACAGTTC
PRIMER_INTERNAL_1_SEQUENCE=TCACACCAAAAATACCTAATTTCAA
PRIMER_LEFT_1=63,20
PRIMER_RIGHT_1=137,20
PRIMER_INTERNAL_1=84,25
PRIMER_LEFT_1_TM=59.476
PRIMER_RIGHT_1_TM=59.703
PRIMER_INTERNAL_1_TM=58.928
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=28.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=1.099059
PRIMER_LEFT_2_PENALTY=0.523704
PRIMER_RIGHT_2_PENALTY=0.575355
PRIMER_INTERNAL_2_PENALTY=0.296722
PRIMER_LEFT_2_SEQUENCE=CCTGCATGAGGGTCATGATA
PRIMER_RIGHT_2_SEQUENCE=TTCCACTTCAGGCCTCCTAG
PRIMER_INTERNAL_2_SEQUENCE=GAACTGTGAAAGCCTGACCC
PRIMER_LEFT_2=63,20
PRIMER_RIGHT_2=160,20
PRIMER_INTERNAL_2=118,20
PRIMER_LEFT_2_TM=59.476
PRIMER_RIGHT_2_TM=59.425
PRIMER_INTERNAL_2_TM=59.703
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=98
PRIMER_PAIR_3_PENALTY=1.605240
PRIMER_LEFT_3_PENALTY=0.523704
PRIMER_RIGHT_3_PENALTY=1.081536
PRIMER_INTERNAL_3_PENALTY=0.296722
PRIMER_LEFT_3_SEQUENCE=CCTGCATGAGGGTCATGATA
PRIMER_RIGHT_3_SEQUENCE=AATTCCACTTCAGGCCTCCTA
PRIMER_INTERNAL_3_SEQUENCE=GAACTGTGAAAGCCTGACCC
PRIMER_LEFT_3=63,20
PRIMER_RIGHT_3=162,21
PRIMER_INTERNAL_3=118,20
PRIMER_LEFT_3_TM=59.476
PRIMER_RIGHT_3_TM=60.082
PRIMER_INTERNAL_3_TM=59.703
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=1.632216
PRIMER_LEFT_4_PENALTY=0.523704
PRIMER_RIGHT_4_PENALTY=1.108512
PRIMER_INTERNAL_4_PENALTY=0.296722
PRIMER_LEFT_4_SEQUENCE=CCTGCATGAGGGTCATGATA
PRIMER_RIGHT_4_SEQUENCE=TCCACTTCAGGCCTCCTAGT
PRIMER_INTERNAL_4_SEQUENCE=GAACTGTGAAAGCCTGACCC
PRIMER_LEFT_4=63,20
PRIMER_RIGHT_4=159,20
PRIMER_INTERNAL_4=118,20
PRIMER_LEFT_4_TM=59.476
PRIMER_RIGHT_4_TM=58.891
PRIMER_INTERNAL_4_TM=59.703
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=97
=
SEQUENCE_ID=MH670
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGTTACGATTCTTTGCACTAAAACATTTTTATGTATCTCATACTTAGGTATTGGCATGTTTATGTTTATAAAACTTCTGATAAATGTTATACATGTCATGAAATTTAAGGGTGAAATATACATACATCTGAAAGTAGTGCCAAAAAACTGAAAATATATTTGCACACTTAAATGTCTTTGTTTCTAGCGATATTCATCTTCAACCATAATAGCCTTTTCTGAAGTGGTTTGTTTGGTTTTTATATTAGGTTTTCGTTGTCAGTGTTCCTTTCTAATTG
SEQUENCE_INCLUDED_REGION=36,281
P3_COMMENT=CL is ATCTCATACTTAGGTATTGGCATG
P3_COMMENT=CR is ATTAGAAAGGAACACTGACAACG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=5.194254
PRIMER_LEFT_0_PENALTY=3.681214
PRIMER_RIGHT_0_PENALTY=1.513039
PRIMER_INTERNAL_0_PENALTY=2.325733
PRIMER_LEFT_0_SEQUENCE=AGCGATATTCATCTTCAACCA
PRIMER_RIGHT_0_SEQUENCE=GGAACACTGACAACGAAAACC
PRIMER_INTERNAL_0_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
PRIMER_LEFT_0=224,21
PRIMER_RIGHT_0=306,21
PRIMER_INTERNAL_0=250,22
PRIMER_LEFT_0_TM=57.319
PRIMER_RIGHT_0_TM=59.487
PRIMER_INTERNAL_0_TM=59.674
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=83
PRIMER_PAIR_1_PENALTY=5.628046
PRIMER_LEFT_1_PENALTY=4.115007
PRIMER_RIGHT_1_PENALTY=1.513039
PRIMER_INTERNAL_1_PENALTY=2.325733
PRIMER_LEFT_1_SEQUENCE=TCTAGCGATATTCATCTTCAACCA
PRIMER_RIGHT_1_SEQUENCE=GGAACACTGACAACGAAAACC
PRIMER_INTERNAL_1_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
PRIMER_LEFT_1=221,24
PRIMER_RIGHT_1=306,21
PRIMER_INTERNAL_1=250,22
PRIMER_LEFT_1_TM=60.115
PRIMER_RIGHT_1_TM=59.487
PRIMER_INTERNAL_1_TM=59.674
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=86
PRIMER_PAIR_2_PENALTY=5.794336
PRIMER_LEFT_2_PENALTY=4.281296
PRIMER_RIGHT_2_PENALTY=1.513039
PRIMER_INTERNAL_2_PENALTY=2.325733
PRIMER_LEFT_2_SEQUENCE=AGCGATATTCATCTTCAACCAT
PRIMER_RIGHT_2_SEQUENCE=GGAACACTGACAACGAAAACC
PRIMER_INTERNAL_2_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
PRIMER_LEFT_2=224,22
PRIMER_RIGHT_2=306,21
PRIMER_INTERNAL_2=250,22
PRIMER_LEFT_2_TM=57.719
PRIMER_RIGHT_2_TM=59.487
PRIMER_INTERNAL_2_TM=59.674
PRIMER_LEFT_2_GC_PERCENT=36.364
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=6.084693
PRIMER_LEFT_3_PENALTY=4.571654
PRIMER_RIGHT_3_PENALTY=1.513039
PRIMER_INTERNAL_3_PENALTY=2.325733
PRIMER_LEFT_3_SEQUENCE=TAGCGATATTCATCTTCAACCA
PRIMER_RIGHT_3_SEQUENCE=GGAACACTGACAACGAAAACC
PRIMER_INTERNAL_3_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
PRIMER_LEFT_3=223,22
PRIMER_RIGHT_3=306,21
PRIMER_INTERNAL_3=250,22
PRIMER_LEFT_3_TM=57.428
PRIMER_RIGHT_3_TM=59.487
PRIMER_INTERNAL_3_TM=59.674
PRIMER_LEFT_3_GC_PERCENT=36.364
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=6.091405
PRIMER_LEFT_4_PENALTY=4.578365
PRIMER_RIGHT_4_PENALTY=1.513039
PRIMER_INTERNAL_4_PENALTY=2.325733
PRIMER_LEFT_4_SEQUENCE=CTAGCGATATTCATCTTCAACCA
PRIMER_RIGHT_4_SEQUENCE=GGAACACTGACAACGAAAACC
PRIMER_INTERNAL_4_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT
PRIMER_LEFT_4=222,23
PRIMER_RIGHT_4=306,21
PRIMER_INTERNAL_4=250,22
PRIMER_LEFT_4_TM=58.422
PRIMER_RIGHT_4_TM=59.487
PRIMER_INTERNAL_4_TM=59.674
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH672
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACCTTGCCAAATGTTCCATTATAAAAGTTATAGTATCAATGATAGAAACTTTAGGAAATTTAAGATGGCGGACATCCTTACAATTTTCTTTGTAAGTACATTTAAAAAAGATGTCTGTGTACTTCAATTGGAAGGGAATGAGGGTTGACTCAAAGCATATAATTAATTATCCTTGGCTATTTTACACTGTGGCTAATTCAGNTGTTACTCTCTCAGGAAGACAGGGAGAAACTTGATTTATTTTGTCTGGAATCCTATTCTTATTAAAAA
SEQUENCE_INCLUDED_REGION=36,273
P3_COMMENT=CL is TACCTTGCCAAATGTTCCAT
P3_COMMENT=CR is TTCTCCCTGTCTTCCTGAGA
P3_COMMENT=CM is GAAACTTTAGGAAATTTAAGATGGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.427758
PRIMER_LEFT_0_PENALTY=0.330865
PRIMER_RIGHT_0_PENALTY=0.096893
PRIMER_INTERNAL_0_PENALTY=5.825088
PRIMER_LEFT_0_SEQUENCE=TGGCGGACATCCTTACAATT
PRIMER_RIGHT_0_SEQUENCE=TTGAGTCAACCCTCATTCCC
PRIMER_INTERNAL_0_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
PRIMER_LEFT_0=104,20
PRIMER_RIGHT_0=191,20
PRIMER_INTERNAL_0=148,24
PRIMER_LEFT_0_TM=60.331
PRIMER_RIGHT_0_TM=59.903
PRIMER_INTERNAL_0_TM=58.175
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.568945
PRIMER_LEFT_1_PENALTY=0.472052
PRIMER_RIGHT_1_PENALTY=0.096893
PRIMER_INTERNAL_1_PENALTY=5.825088
PRIMER_LEFT_1_SEQUENCE=TTAAGATGGCGGACATCCTT
PRIMER_RIGHT_1_SEQUENCE=TTGAGTCAACCCTCATTCCC
PRIMER_INTERNAL_1_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
PRIMER_LEFT_1=98,20
PRIMER_RIGHT_1=191,20
PRIMER_INTERNAL_1=148,24
PRIMER_LEFT_1_TM=59.528
PRIMER_RIGHT_1_TM=59.903
PRIMER_INTERNAL_1_TM=58.175
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=0.568945
PRIMER_LEFT_2_PENALTY=0.472052
PRIMER_RIGHT_2_PENALTY=0.096893
PRIMER_INTERNAL_2_PENALTY=5.825088
PRIMER_LEFT_2_SEQUENCE=TTTAAGATGGCGGACATCCT
PRIMER_RIGHT_2_SEQUENCE=TTGAGTCAACCCTCATTCCC
PRIMER_INTERNAL_2_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
PRIMER_LEFT_2=97,20
PRIMER_RIGHT_2=191,20
PRIMER_INTERNAL_2=148,24
PRIMER_LEFT_2_TM=59.528
PRIMER_RIGHT_2_TM=59.903
PRIMER_INTERNAL_2_TM=58.175
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=0.579555
PRIMER_LEFT_3_PENALTY=0.482662
PRIMER_RIGHT_3_PENALTY=0.096893
PRIMER_INTERNAL_3_PENALTY=5.825088
PRIMER_LEFT_3_SEQUENCE=AGATGGCGGACATCCTTACA
PRIMER_RIGHT_3_SEQUENCE=TTGAGTCAACCCTCATTCCC
PRIMER_INTERNAL_3_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
PRIMER_LEFT_3=101,20
PRIMER_RIGHT_3=191,20
PRIMER_INTERNAL_3=148,24
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=59.903
PRIMER_INTERNAL_3_TM=58.175
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=0.824197
PRIMER_LEFT_4_PENALTY=0.727304
PRIMER_RIGHT_4_PENALTY=0.096893
PRIMER_INTERNAL_4_PENALTY=5.825088
PRIMER_LEFT_4_SEQUENCE=ATGGCGGACATCCTTACAAT
PRIMER_RIGHT_4_SEQUENCE=TTGAGTCAACCCTCATTCCC
PRIMER_INTERNAL_4_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA
PRIMER_LEFT_4=103,20
PRIMER_RIGHT_4=191,20
PRIMER_INTERNAL_4=148,24
PRIMER_LEFT_4_TM=59.273
PRIMER_RIGHT_4_TM=59.903
PRIMER_INTERNAL_4_TM=58.175
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH679
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCACAGTCTCCTTTCCTTCTGCTCAGTACATTGAAGACGTAGTTTCTTTCATTTTGAGGACTTCTCTTAAGCAAAATATTTGAGGACTAACTAATAAATATTTAGGAAAAACTTGTCTCAGTACAAGTGGACATTTTTGTNATTTCTTTGGTCCTCACTCCAACCATCATTTCCCCCTTCTATGCCATTCATCTTTAACAAAATAAGTTTTCCTGTTGCCAGGGAGGTAAAACTTTCACTCTACCCTCTTAGGTTCAGTATTCGGGGGATTGCAAATTAAACCAACAAAACACAGCTCCACCCCATCAACATCTGGGG
SEQUENCE_INCLUDED_REGION=36,319
P3_COMMENT=CL is TGCACAGTCTCCTTTCCTTC
P3_COMMENT=CR is GGAGCTGTGTTTTGTTGGTT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.041723
PRIMER_LEFT_0_PENALTY=0.029253
PRIMER_RIGHT_0_PENALTY=0.012470
PRIMER_INTERNAL_0_PENALTY=0.017342
PRIMER_LEFT_0_SEQUENCE=TGTTGCCAGGGAGGTAAAAC
PRIMER_RIGHT_0_SEQUENCE=GGGTGGAGCTGTGTTTTGTT
PRIMER_INTERNAL_0_SEQUENCE=GGTTCAGTATTCGGGGGATT
PRIMER_LEFT_0=250,20
PRIMER_RIGHT_0=339,20
PRIMER_INTERNAL_0=288,20
PRIMER_LEFT_0_TM=59.971
PRIMER_RIGHT_0_TM=60.012
PRIMER_INTERNAL_0_TM=60.017
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=0.115494
PRIMER_LEFT_1_PENALTY=0.103024
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_INTERNAL_1_PENALTY=0.017342
PRIMER_LEFT_1_SEQUENCE=CTGTTGCCAGGGAGGTAAAA
PRIMER_RIGHT_1_SEQUENCE=GGGTGGAGCTGTGTTTTGTT
PRIMER_INTERNAL_1_SEQUENCE=GGTTCAGTATTCGGGGGATT
PRIMER_LEFT_1=249,20
PRIMER_RIGHT_1=339,20
PRIMER_INTERNAL_1=288,20
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=60.012
PRIMER_INTERNAL_1_TM=60.017
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.201877
PRIMER_LEFT_2_PENALTY=0.172624
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_INTERNAL_2_PENALTY=5.490789
PRIMER_LEFT_2_SEQUENCE=CACTCCAACCATCATTTCCC
PRIMER_RIGHT_2_SEQUENCE=GTTTTACCTCCCTGGCAACA
PRIMER_INTERNAL_2_SEQUENCE=CCTTCTATGCCATTCATCTTTAA
PRIMER_LEFT_2=192,20
PRIMER_RIGHT_2=269,20
PRIMER_INTERNAL_2=212,23
PRIMER_LEFT_2_TM=60.173
PRIMER_RIGHT_2_TM=59.971
PRIMER_INTERNAL_2_TM=57.509
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=0.275648
PRIMER_LEFT_3_PENALTY=0.172624
PRIMER_RIGHT_3_PENALTY=0.103024
PRIMER_INTERNAL_3_PENALTY=5.490789
PRIMER_LEFT_3_SEQUENCE=CACTCCAACCATCATTTCCC
PRIMER_RIGHT_3_SEQUENCE=TTTTACCTCCCTGGCAACAG
PRIMER_INTERNAL_3_SEQUENCE=CCTTCTATGCCATTCATCTTTAA
PRIMER_LEFT_3=192,20
PRIMER_RIGHT_3=268,20
PRIMER_INTERNAL_3=212,23
PRIMER_LEFT_3_TM=60.173
PRIMER_RIGHT_3_TM=60.103
PRIMER_INTERNAL_3_TM=57.509
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=0.392242
PRIMER_LEFT_4_PENALTY=0.362989
PRIMER_RIGHT_4_PENALTY=0.029253
PRIMER_INTERNAL_4_PENALTY=0.372603
PRIMER_LEFT_4_SEQUENCE=TTGGTCCTCACTCCAACCAT
PRIMER_RIGHT_4_SEQUENCE=GTTTTACCTCCCTGGCAACA
PRIMER_INTERNAL_4_SEQUENCE=ATTTCCCCCTTCTATGCCAT
PRIMER_LEFT_4=184,20
PRIMER_RIGHT_4=269,20
PRIMER_INTERNAL_4=205,20
PRIMER_LEFT_4_TM=60.363
PRIMER_RIGHT_4_TM=59.971
PRIMER_INTERNAL_4_TM=59.627
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH683
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTCAATGAAACAAGACATTAGGAAAGAGAATGCAGAAAAAGTGTTACTTTGTCTACTTTAAATTTTCAAGTTGTACATTATCTATTCTCGTTTATCTCTGGGTNAATGACGATACCTTTGTCAGGGTGCGGACAGTAGGATAATAAGTAAGTTGTAAAGCCCAGTACACTTCTCTTGGCACTGAAGCAAATCAATCCCCTGAAATACACTGAAGACAATGGATGATCATTAAGATTTTCAGACAGGGTTTAAACGCATTAAAATGTGAAAA
SEQUENCE_INCLUDED_REGION=35,275
P3_COMMENT=CL is CAAGACATTAGGAAAGAGAATGC
P3_COMMENT=CR is GCGTTTAAACCCTGTCTGAA
P3_COMMENT=CM is TTATCTATTCTCGTTTATCTCTGGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.058439
PRIMER_LEFT_0_PENALTY=0.044832
PRIMER_RIGHT_0_PENALTY=0.013607
PRIMER_INTERNAL_0_PENALTY=1.297410
PRIMER_LEFT_0_SEQUENCE=GGGTGCGGACAGTAGGATAA
PRIMER_RIGHT_0_SEQUENCE=TCAGGGGATTGATTTGCTTC
PRIMER_INTERNAL_0_SEQUENCE=CCCAGTACACTTCTCTTGGCA
PRIMER_LEFT_0=162,20
PRIMER_RIGHT_0=240,20
PRIMER_INTERNAL_0=198,21
PRIMER_LEFT_0_TM=59.955
PRIMER_RIGHT_0_TM=60.014
PRIMER_INTERNAL_0_TM=60.297
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=79
PRIMER_PAIR_1_PENALTY=0.439693
PRIMER_LEFT_1_PENALTY=0.426086
PRIMER_RIGHT_1_PENALTY=0.013607
PRIMER_INTERNAL_1_PENALTY=1.297410
PRIMER_LEFT_1_SEQUENCE=AGGGTGCGGACAGTAGGATA
PRIMER_RIGHT_1_SEQUENCE=TCAGGGGATTGATTTGCTTC
PRIMER_INTERNAL_1_SEQUENCE=CCCAGTACACTTCTCTTGGCA
PRIMER_LEFT_1=161,20
PRIMER_RIGHT_1=240,20
PRIMER_INTERNAL_1=198,21
PRIMER_LEFT_1_TM=59.574
PRIMER_RIGHT_1_TM=60.014
PRIMER_INTERNAL_1_TM=60.297
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=80
PRIMER_PAIR_2_PENALTY=0.528743
PRIMER_LEFT_2_PENALTY=0.516683
PRIMER_RIGHT_2_PENALTY=0.012060
PRIMER_INTERNAL_2_PENALTY=5.952353
PRIMER_LEFT_2_SEQUENCE=GATACCTTTGTCAGGGTGCG
PRIMER_RIGHT_2_SEQUENCE=TGATTTGCTTCAGTGCCAAG
PRIMER_INTERNAL_2_SEQUENCE=AAGTTGTAAAGCCCAGTACACTTC
PRIMER_LEFT_2=149,20
PRIMER_RIGHT_2=231,20
PRIMER_INTERNAL_2=187,24
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=59.988
PRIMER_INTERNAL_2_TM=58.048
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.528743
PRIMER_LEFT_3_PENALTY=0.516683
PRIMER_RIGHT_3_PENALTY=0.012060
PRIMER_INTERNAL_3_PENALTY=5.952353
PRIMER_LEFT_3_SEQUENCE=CGATACCTTTGTCAGGGTGC
PRIMER_RIGHT_3_SEQUENCE=TGATTTGCTTCAGTGCCAAG
PRIMER_INTERNAL_3_SEQUENCE=AAGTTGTAAAGCCCAGTACACTTC
PRIMER_LEFT_3=148,20
PRIMER_RIGHT_3=231,20
PRIMER_INTERNAL_3=187,24
PRIMER_LEFT_3_TM=60.517
PRIMER_RIGHT_3_TM=59.988
PRIMER_INTERNAL_3_TM=58.048
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=0.530290
PRIMER_LEFT_4_PENALTY=0.516683
PRIMER_RIGHT_4_PENALTY=0.013607
PRIMER_INTERNAL_4_PENALTY=1.297410
PRIMER_LEFT_4_SEQUENCE=GATACCTTTGTCAGGGTGCG
PRIMER_RIGHT_4_SEQUENCE=TCAGGGGATTGATTTGCTTC
PRIMER_INTERNAL_4_SEQUENCE=CCCAGTACACTTCTCTTGGCA
PRIMER_LEFT_4=149,20
PRIMER_RIGHT_4=240,20
PRIMER_INTERNAL_4=198,21
PRIMER_LEFT_4_TM=60.517
PRIMER_RIGHT_4_TM=60.014
PRIMER_INTERNAL_4_TM=60.297
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH684
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAGGTGGACCAGTAGCGTTCTGTGGCCTGGGGGCGGAGGCCTCTAAGACACGGGTTGTGGCTATAAGATGAATGACAGCCATAAGGCCCACTCTGACATCTCTCGGGGCCGATGCACTATGTATCACTTAGCAAGATGCTGTAACCTGGTCTGGGGATTTCCAACCCTGGTCTGGGGAATTACTGGGTCTCCATGACAACAGGACCTAAAAAACCTGGTTGCCCTAGAGACAAGGCCTCCTCAGCACATATGCAACCTTCTTGAACCTTAAAACCAACCTTACTCTTACA
SEQUENCE_INCLUDED_REGION=35,292
P3_COMMENT=CL is GTGGACCAGTAGCGTTCTGT
P3_COMMENT=CR is AGGTCCTGTTGTCATGGAGA
P3_COMMENT=CM is GGGCGGAGGCCTCTAAGA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.218298
PRIMER_LEFT_0_PENALTY=0.051910
PRIMER_RIGHT_0_PENALTY=0.166387
PRIMER_INTERNAL_0_PENALTY=0.934117
PRIMER_LEFT_0_SEQUENCE=ACCCTGGTCTGGGGAATTAC
PRIMER_RIGHT_0_SEQUENCE=AGGAGGCCTTGTCTCTAGGG
PRIMER_INTERNAL_0_SEQUENCE=GGGTCTCCATGACAACAGGA
PRIMER_LEFT_0=200,20
PRIMER_RIGHT_0=277,20
PRIMER_INTERNAL_0=221,20
PRIMER_LEFT_0_TM=60.052
PRIMER_RIGHT_0_TM=59.834
PRIMER_INTERNAL_0_TM=60.934
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=78
PRIMER_PAIR_1_PENALTY=0.228966
PRIMER_LEFT_1_PENALTY=0.051910
PRIMER_RIGHT_1_PENALTY=0.177056
PRIMER_INTERNAL_1_PENALTY=0.246438
PRIMER_LEFT_1_SEQUENCE=GGTCTGGGGAATTACTGGGT
PRIMER_RIGHT_1_SEQUENCE=TTGCATATGTGCTGAGGAGG
PRIMER_INTERNAL_1_SEQUENCE=TGGTTGCCCTAGAGACAAGG
PRIMER_LEFT_1=205,20
PRIMER_RIGHT_1=291,20
PRIMER_INTERNAL_1=252,20
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=59.823
PRIMER_INTERNAL_1_TM=60.246
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=0.228966
PRIMER_LEFT_2_PENALTY=0.051910
PRIMER_RIGHT_2_PENALTY=0.177056
PRIMER_INTERNAL_2_PENALTY=0.246438
PRIMER_LEFT_2_SEQUENCE=ACCCTGGTCTGGGGAATTAC
PRIMER_RIGHT_2_SEQUENCE=TTGCATATGTGCTGAGGAGG
PRIMER_INTERNAL_2_SEQUENCE=TGGTTGCCCTAGAGACAAGG
PRIMER_LEFT_2=200,20
PRIMER_RIGHT_2=291,20
PRIMER_INTERNAL_2=252,20
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=59.823
PRIMER_INTERNAL_2_TM=60.246
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=0.253140
PRIMER_LEFT_3_PENALTY=0.201230
PRIMER_RIGHT_3_PENALTY=0.051910
PRIMER_INTERNAL_3_PENALTY=0.010545
PRIMER_LEFT_3_SEQUENCE=GGCCCACTCTGACATCTCTC
PRIMER_RIGHT_3_SEQUENCE=GTAATTCCCCAGACCAGGGT
PRIMER_INTERNAL_3_SEQUENCE=ATGCTGTAACCTGGTCTGGG
PRIMER_LEFT_3=121,20
PRIMER_RIGHT_3=219,20
PRIMER_INTERNAL_3=172,20
PRIMER_LEFT_3_TM=59.799
PRIMER_RIGHT_3_TM=60.052
PRIMER_INTERNAL_3_TM=59.989
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=0.256983
PRIMER_LEFT_4_PENALTY=0.010545
PRIMER_RIGHT_4_PENALTY=0.246438
PRIMER_INTERNAL_4_PENALTY=0.051910
PRIMER_LEFT_4_SEQUENCE=ATGCTGTAACCTGGTCTGGG
PRIMER_RIGHT_4_SEQUENCE=TTGTCTCTAGGGCAACCAGG
PRIMER_INTERNAL_4_SEQUENCE=GGTCTGGGGAATTACTGGGT
PRIMER_LEFT_4=172,20
PRIMER_RIGHT_4=269,20
PRIMER_INTERNAL_4=205,20
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=60.246
PRIMER_INTERNAL_4_TM=60.052
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH688
SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAAAGATTTTTAAAGTGTTTGTTTGGAATGTGTCAAGAGTNNTTTTGATGTGATATATCTAAGGTNACATGCAGAATCACTGAAATATTAAATGCCAAATGGTTTGCAAATAAACATGCAGATAATTATTTTNCTTCCCTAAATTTCAAGTTGGCACTAGCCAGCACTCTTGCCAAATGTTCCATCTATTTTCTTCCATTCTCAGCATCTTGCATGCTACCGTCCACGCTTCTCATAGTGATGCCTCATTACTGATAAGTTGTAATATCACTTGGTTGCCTATGACATTGAATCCAAG
SEQUENCE_INCLUDED_REGION=35,300
P3_COMMENT=CL is TTAAATGCCAAATGGTTTGC
P3_COMMENT=CR is TGTCATAGGCAACCAAGTGA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.357620
PRIMER_LEFT_0_PENALTY=0.080808
PRIMER_RIGHT_0_PENALTY=0.276812
PRIMER_INTERNAL_0_PENALTY=0.503684
PRIMER_LEFT_0_SEQUENCE=AGTTGGCACTAGCCAGCACT
PRIMER_RIGHT_0_SEQUENCE=ATGAGAAGCGTGGACGGTAG
PRIMER_INTERNAL_0_SEQUENCE=TCCATTCTCAGCATCTTGCA
PRIMER_LEFT_0=185,20
PRIMER_RIGHT_0=272,20
PRIMER_INTERNAL_0=231,20
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=60.277
PRIMER_INTERNAL_0_TM=60.504
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.357620
PRIMER_LEFT_1_PENALTY=0.080808
PRIMER_RIGHT_1_PENALTY=0.276812
PRIMER_INTERNAL_1_PENALTY=0.296338
PRIMER_LEFT_1_SEQUENCE=AGTTGGCACTAGCCAGCACT
PRIMER_RIGHT_1_SEQUENCE=CTATGAGAAGCGTGGACGGT
PRIMER_INTERNAL_1_SEQUENCE=TTCTCAGCATCTTGCATGCT
PRIMER_LEFT_1=185,20
PRIMER_RIGHT_1=274,20
PRIMER_INTERNAL_1=235,20
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.277
PRIMER_INTERNAL_1_TM=59.704
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=0.357620
PRIMER_LEFT_2_PENALTY=0.080808
PRIMER_RIGHT_2_PENALTY=0.276812
PRIMER_INTERNAL_2_PENALTY=0.296338
PRIMER_LEFT_2_SEQUENCE=AGTTGGCACTAGCCAGCACT
PRIMER_RIGHT_2_SEQUENCE=ACTATGAGAAGCGTGGACGG
PRIMER_INTERNAL_2_SEQUENCE=TTCTCAGCATCTTGCATGCT
PRIMER_LEFT_2=185,20
PRIMER_RIGHT_2=275,20
PRIMER_INTERNAL_2=235,20
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.277
PRIMER_INTERNAL_2_TM=59.704
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_3_PENALTY=0.372827
PRIMER_LEFT_3_PENALTY=0.064105
PRIMER_RIGHT_3_PENALTY=0.308722
PRIMER_INTERNAL_3_PENALTY=0.536418
PRIMER_LEFT_3_SEQUENCE=TGCCAAATGGTTTGCAAATA
PRIMER_RIGHT_3_SEQUENCE=TGGAACATTTGGCAAGAGTG
PRIMER_INTERNAL_3_SEQUENCE=TTTCAAGTTGGCACTAGCCA
PRIMER_LEFT_3=129,20
PRIMER_RIGHT_3=220,20
PRIMER_INTERNAL_3=180,20
PRIMER_LEFT_3_TM=59.936
PRIMER_RIGHT_3_TM=59.691
PRIMER_INTERNAL_3_TM=59.464
PRIMER_LEFT_3_GC_PERCENT=35.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=9.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=0.372827
PRIMER_LEFT_4_PENALTY=0.064105
PRIMER_RIGHT_4_PENALTY=0.308722
PRIMER_INTERNAL_4_PENALTY=0.536418
PRIMER_LEFT_4_SEQUENCE=TAAATGCCAAATGGTTTGCA
PRIMER_RIGHT_4_SEQUENCE=TGGAACATTTGGCAAGAGTG
PRIMER_INTERNAL_4_SEQUENCE=TTTCAAGTTGGCACTAGCCA
PRIMER_LEFT_4=125,20
PRIMER_RIGHT_4=220,20
PRIMER_INTERNAL_4=180,20
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=59.691
PRIMER_INTERNAL_4_TM=59.464
PRIMER_LEFT_4_GC_PERCENT=35.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=9.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH691
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCATGCCCCACAAAAACATACTTTTTGTGCTCCTGTTTATTAACTGGTACTTACTAAAAGCCAAATACTCCTAGAACTTTGTAGTATTATCCCTTTTAATTCCCACATCAACTAAGGTAGATGCTGTCTGTTATTGACTAAATTGTGTCTTCCCACAAAATTCAAATGTTGTAGCCCTAACTCTCAATGTGATGGTATTTAGAGACGGGGCTTTTGGGAGGTCATTAGGATTACATGAGGTCGTGGG
SEQUENCE_INCLUDED_REGION=34,118
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.127760
PRIMER_LEFT_0_PENALTY=4.387445
PRIMER_RIGHT_0_PENALTY=1.740315
PRIMER_INTERNAL_0_PENALTY=6.319798
PRIMER_LEFT_0_SEQUENCE=TTTGTGCTCCTGTTTATTAACTGG
PRIMER_RIGHT_0_SEQUENCE=TGATGTGGGAATTAAAAGGGA
PRIMER_INTERNAL_0_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
PRIMER_LEFT_0=60,24
PRIMER_RIGHT_0=146,21
PRIMER_INTERNAL_0=92,26
PRIMER_LEFT_0_TM=59.613
PRIMER_RIGHT_0_TM=59.260
PRIMER_INTERNAL_0_TM=59.680
PRIMER_LEFT_0_GC_PERCENT=37.500
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=34.615
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=8.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=6.357965
PRIMER_LEFT_1_PENALTY=4.617650
PRIMER_RIGHT_1_PENALTY=1.740315
PRIMER_INTERNAL_1_PENALTY=6.319798
PRIMER_LEFT_1_SEQUENCE=TTGTGCTCCTGTTTATTAACTGG
PRIMER_RIGHT_1_SEQUENCE=TGATGTGGGAATTAAAAGGGA
PRIMER_INTERNAL_1_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
PRIMER_LEFT_1=61,23
PRIMER_RIGHT_1=146,21
PRIMER_INTERNAL_1=92,26
PRIMER_LEFT_1_TM=58.382
PRIMER_RIGHT_1_TM=59.260
PRIMER_INTERNAL_1_TM=59.680
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_INTERNAL_1_GC_PERCENT=34.615
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=8.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=86
PRIMER_PAIR_2_PENALTY=6.578207
PRIMER_LEFT_2_PENALTY=4.837892
PRIMER_RIGHT_2_PENALTY=1.740315
PRIMER_INTERNAL_2_PENALTY=6.319798
PRIMER_LEFT_2_SEQUENCE=TTGTGCTCCTGTTTATTAACTGGT
PRIMER_RIGHT_2_SEQUENCE=TGATGTGGGAATTAAAAGGGA
PRIMER_INTERNAL_2_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
PRIMER_LEFT_2=61,24
PRIMER_RIGHT_2=146,21
PRIMER_INTERNAL_2=92,26
PRIMER_LEFT_2_TM=59.162
PRIMER_RIGHT_2_TM=59.260
PRIMER_INTERNAL_2_TM=59.680
PRIMER_LEFT_2_GC_PERCENT=37.500
PRIMER_RIGHT_2_GC_PERCENT=38.095
PRIMER_INTERNAL_2_GC_PERCENT=34.615
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=6.593719
PRIMER_LEFT_3_PENALTY=4.387445
PRIMER_RIGHT_3_PENALTY=2.206274
PRIMER_INTERNAL_3_PENALTY=6.319798
PRIMER_LEFT_3_SEQUENCE=TTTGTGCTCCTGTTTATTAACTGG
PRIMER_RIGHT_3_SEQUENCE=TTGATGTGGGAATTAAAAGGG
PRIMER_INTERNAL_3_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
PRIMER_LEFT_3=60,24
PRIMER_RIGHT_3=147,21
PRIMER_INTERNAL_3=92,26
PRIMER_LEFT_3_TM=59.613
PRIMER_RIGHT_3_TM=58.794
PRIMER_INTERNAL_3_TM=59.680
PRIMER_LEFT_3_GC_PERCENT=37.500
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_INTERNAL_3_GC_PERCENT=34.615
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=6.716551
PRIMER_LEFT_4_PENALTY=4.976237
PRIMER_RIGHT_4_PENALTY=1.740315
PRIMER_INTERNAL_4_PENALTY=6.319798
PRIMER_LEFT_4_SEQUENCE=TGTGCTCCTGTTTATTAACTGG
PRIMER_RIGHT_4_SEQUENCE=TGATGTGGGAATTAAAAGGGA
PRIMER_INTERNAL_4_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=146,21
PRIMER_INTERNAL_4=92,26
PRIMER_LEFT_4_TM=57.024
PRIMER_RIGHT_4_TM=59.260
PRIMER_INTERNAL_4_TM=59.680
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=34.615
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=8.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=85
=
SEQUENCE_ID=MH692
SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAAAAGAGGNNTAAAGAAACTAAAAGCAACAAAACCAAACTTAAAAAAAAAAAGGCAAAGGTTCTGAACTGCAAAGGAAATTGTCATACCATTAACTCTGCTGAATGAAAGAGGAATACAGCCTCCTCCGGTTCTTACAGTTGTGTATAAAATAAGGTAGCAGATTAGAAATAGAGAAGATATGCTAAAGATGAGATGTGACTGATGACAGCAGACAGCAAAGGAAGGAAGTATAATTGCTAACATGAAATAAAAGTGCTACAG
SEQUENCE_INCLUDED_REGION=36,267
P3_COMMENT=CL is GGTTCTGAACTGCAAAGGAA
P3_COMMENT=CR is TGTAGCACTTTTATTTCATGTTAGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.618156
PRIMER_LEFT_0_PENALTY=0.522443
PRIMER_RIGHT_0_PENALTY=0.095713
PRIMER_INTERNAL_0_PENALTY=3.799488
PRIMER_LEFT_0_SEQUENCE=AGGCAAAGGTTCTGAACTGC
PRIMER_RIGHT_0_SEQUENCE=AGAACCGGAGGAGGCTGTAT
PRIMER_INTERNAL_0_SEQUENCE=AACTCTGCTGAATGAAAGAGGA
PRIMER_LEFT_0=91,20
PRIMER_RIGHT_0=173,20
PRIMER_INTERNAL_0=132,22
PRIMER_LEFT_0_TM=59.478
PRIMER_RIGHT_0_TM=60.096
PRIMER_INTERNAL_0_TM=58.201
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=5.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=83
PRIMER_PAIR_1_PENALTY=0.986123
PRIMER_LEFT_1_PENALTY=0.522443
PRIMER_RIGHT_1_PENALTY=0.463680
PRIMER_INTERNAL_1_PENALTY=4.206859
PRIMER_LEFT_1_SEQUENCE=AGGCAAAGGTTCTGAACTGC
PRIMER_RIGHT_1_SEQUENCE=AACCGGAGGAGGCTGTATTC
PRIMER_INTERNAL_1_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA
PRIMER_LEFT_1=91,20
PRIMER_RIGHT_1=171,20
PRIMER_INTERNAL_1=127,24
PRIMER_LEFT_1_TM=59.478
PRIMER_RIGHT_1_TM=60.464
PRIMER_INTERNAL_1_TM=59.793
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=81
PRIMER_PAIR_2_PENALTY=0.986123
PRIMER_LEFT_2_PENALTY=0.522443
PRIMER_RIGHT_2_PENALTY=0.463680
PRIMER_INTERNAL_2_PENALTY=4.206859
PRIMER_LEFT_2_SEQUENCE=AGGCAAAGGTTCTGAACTGC
PRIMER_RIGHT_2_SEQUENCE=GAACCGGAGGAGGCTGTATT
PRIMER_INTERNAL_2_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA
PRIMER_LEFT_2=91,20
PRIMER_RIGHT_2=172,20
PRIMER_INTERNAL_2=127,24
PRIMER_LEFT_2_TM=59.478
PRIMER_RIGHT_2_TM=60.464
PRIMER_INTERNAL_2_TM=59.793
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=1.045650
PRIMER_LEFT_3_PENALTY=0.949937
PRIMER_RIGHT_3_PENALTY=0.095713
PRIMER_INTERNAL_3_PENALTY=3.799488
PRIMER_LEFT_3_SEQUENCE=GCAAAGGTTCTGAACTGCAA
PRIMER_RIGHT_3_SEQUENCE=AGAACCGGAGGAGGCTGTAT
PRIMER_INTERNAL_3_SEQUENCE=AACTCTGCTGAATGAAAGAGGA
PRIMER_LEFT_3=93,20
PRIMER_RIGHT_3=173,20
PRIMER_INTERNAL_3=132,22
PRIMER_LEFT_3_TM=59.050
PRIMER_RIGHT_3_TM=60.096
PRIMER_INTERNAL_3_TM=58.201
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=1.413617
PRIMER_LEFT_4_PENALTY=0.949937
PRIMER_RIGHT_4_PENALTY=0.463680
PRIMER_INTERNAL_4_PENALTY=4.206859
PRIMER_LEFT_4_SEQUENCE=GCAAAGGTTCTGAACTGCAA
PRIMER_RIGHT_4_SEQUENCE=AACCGGAGGAGGCTGTATTC
PRIMER_INTERNAL_4_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA
PRIMER_LEFT_4=93,20
PRIMER_RIGHT_4=171,20
PRIMER_INTERNAL_4=127,24
PRIMER_LEFT_4_TM=59.050
PRIMER_RIGHT_4_TM=60.464
PRIMER_INTERNAL_4_TM=59.793
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH693
SEQUENCE_TEMPLATE=GTGTGGGGGCTGGGATGAGAGGTCTACTTGTGGTACTGGAGGTTTCACTGGCTTGTGCTAGAACTAGNAAAGNAGAAAGAGACAGNGATTGGCTAACGCATGGCAGTAGTGGGCCCCAAGGCCTGAGTAATAAGAAAAAATCATTAGATAAATGTCTCATGACCAAAACAAAGTTCAAACANTAGGTGCAGCACANNNGGGTTTTCTCTGGTCATAGAATCTCTTAAAAGGGAATCATGACAGATTTTCTTGGCTTTAAGTCCTTTATTTAGTTCCCTCTACCAAGTGTATCTTTTAAAGCCTTGTTAAGA
SEQUENCE_INCLUDED_REGION=0,311
P3_COMMENT=CL is GGCTGGGATGAGAGGTCTAC
P3_COMMENT=CR is TAACAAGGCTTTAAAAGATACACTT
P3_COMMENT=CM is TGTGGTACTGGAGGTTTCACT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.045873
PRIMER_LEFT_0_PENALTY=0.085951
PRIMER_RIGHT_0_PENALTY=0.959922
PRIMER_INTERNAL_0_PENALTY=0.299723
PRIMER_LEFT_0_SEQUENCE=AGGTTTCACTGGCTTGTGCT
PRIMER_RIGHT_0_SEQUENCE=TTATTACTCAGGCCTTGGGG
PRIMER_INTERNAL_0_SEQUENCE=GGCTAACGCATGGCAGTAGT
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=132,20
PRIMER_INTERNAL_0=90,20
PRIMER_LEFT_0_TM=59.914
PRIMER_RIGHT_0_TM=59.040
PRIMER_INTERNAL_0_TM=60.300
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=1.119235
PRIMER_LEFT_1_PENALTY=0.085951
PRIMER_RIGHT_1_PENALTY=1.033284
PRIMER_INTERNAL_1_PENALTY=0.299723
PRIMER_LEFT_1_SEQUENCE=AGGTTTCACTGGCTTGTGCT
PRIMER_RIGHT_1_SEQUENCE=CTTATTACTCAGGCCTTGGGG
PRIMER_INTERNAL_1_SEQUENCE=GGCTAACGCATGGCAGTAGT
PRIMER_LEFT_1=39,20
PRIMER_RIGHT_1=133,21
PRIMER_INTERNAL_1=90,20
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.967
PRIMER_INTERNAL_1_TM=60.300
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=1.406905
PRIMER_LEFT_2_PENALTY=0.085951
PRIMER_RIGHT_2_PENALTY=1.320954
PRIMER_INTERNAL_2_PENALTY=0.299723
PRIMER_LEFT_2_SEQUENCE=AGGTTTCACTGGCTTGTGCT
PRIMER_RIGHT_2_SEQUENCE=TATTACTCAGGCCTTGGGGC
PRIMER_INTERNAL_2_SEQUENCE=GGCTAACGCATGGCAGTAGT
PRIMER_LEFT_2=39,20
PRIMER_RIGHT_2=131,20
PRIMER_INTERNAL_2=90,20
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=61.321
PRIMER_INTERNAL_2_TM=60.300
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=12.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=93
PRIMER_PAIR_3_PENALTY=1.649603
PRIMER_LEFT_3_PENALTY=0.689681
PRIMER_RIGHT_3_PENALTY=0.959922
PRIMER_INTERNAL_3_PENALTY=0.299723
PRIMER_LEFT_3_SEQUENCE=GGAGGTTTCACTGGCTTGTG
PRIMER_RIGHT_3_SEQUENCE=TTATTACTCAGGCCTTGGGG
PRIMER_INTERNAL_3_SEQUENCE=GGCTAACGCATGGCAGTAGT
PRIMER_LEFT_3=37,20
PRIMER_RIGHT_3=132,20
PRIMER_INTERNAL_3=90,20
PRIMER_LEFT_3_TM=60.690
PRIMER_RIGHT_3_TM=59.040
PRIMER_INTERNAL_3_TM=60.300
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=1.649603
PRIMER_LEFT_4_PENALTY=0.689681
PRIMER_RIGHT_4_PENALTY=0.959922
PRIMER_INTERNAL_4_PENALTY=0.299723
PRIMER_LEFT_4_SEQUENCE=TGGAGGTTTCACTGGCTTGT
PRIMER_RIGHT_4_SEQUENCE=TTATTACTCAGGCCTTGGGG
PRIMER_INTERNAL_4_SEQUENCE=GGCTAACGCATGGCAGTAGT
PRIMER_LEFT_4=36,20
PRIMER_RIGHT_4=132,20
PRIMER_INTERNAL_4=90,20
PRIMER_LEFT_4_TM=60.690
PRIMER_RIGHT_4_TM=59.040
PRIMER_INTERNAL_4_TM=60.300
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=97
=
SEQUENCE_ID=MH694
SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATGTTTATGTTGATATAACTCAAGCCCAGGCACCATTTCTCCTCATTTTCCTTTCAATGTGCTTTTCAAAAATTATGGCATATGTCTGCCTGAAAGTCGGAAACATTTTTGACAATAACTTGGAACTTTCTCTAACCTAATACACTAGATTTAAAGTGAATTATACCGCCATAGGTTGACATGGAGCCATTTTAATAGCAGGTAGTTGACCTGAGAACTCCAAAGCAATTGCTTACATATTGAATTATCTTATA
SEQUENCE_INCLUDED_REGION=36,215
P3_COMMENT=CL is TGTTTATGTTGATATAACTCAAGCC
P3_COMMENT=CR is ACCTGCTATTAAAATGGCTCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.448041
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=0.375090
PRIMER_INTERNAL_0_PENALTY=2.263560
PRIMER_LEFT_0_SEQUENCE=CAGGCACCATTTCTCCTCAT
PRIMER_RIGHT_0_SEQUENCE=TTTCCGACTTTCAGGCAGAC
PRIMER_INTERNAL_0_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
PRIMER_LEFT_0=69,20
PRIMER_RIGHT_0=145,20
PRIMER_INTERNAL_0=91,22
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=60.375
PRIMER_INTERNAL_0_TM=59.736
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=31.818
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=0.448041
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.375090
PRIMER_INTERNAL_1_PENALTY=2.263560
PRIMER_LEFT_1_SEQUENCE=CAGGCACCATTTCTCCTCAT
PRIMER_RIGHT_1_SEQUENCE=GTTTCCGACTTTCAGGCAGA
PRIMER_INTERNAL_1_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
PRIMER_LEFT_1=69,20
PRIMER_RIGHT_1=146,20
PRIMER_INTERNAL_1=91,22
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=60.375
PRIMER_INTERNAL_1_TM=59.736
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=31.818
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=0.708984
PRIMER_LEFT_2_PENALTY=0.072951
PRIMER_RIGHT_2_PENALTY=0.636033
PRIMER_INTERNAL_2_PENALTY=2.263560
PRIMER_LEFT_2_SEQUENCE=CAGGCACCATTTCTCCTCAT
PRIMER_RIGHT_2_SEQUENCE=ATGTTTCCGACTTTCAGGCA
PRIMER_INTERNAL_2_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
PRIMER_LEFT_2=69,20
PRIMER_RIGHT_2=148,20
PRIMER_INTERNAL_2=91,22
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=60.636
PRIMER_INTERNAL_2_TM=59.736
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=31.818
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=0.874143
PRIMER_LEFT_3_PENALTY=0.072951
PRIMER_RIGHT_3_PENALTY=0.801192
PRIMER_INTERNAL_3_PENALTY=2.263560
PRIMER_LEFT_3_SEQUENCE=CAGGCACCATTTCTCCTCAT
PRIMER_RIGHT_3_SEQUENCE=TCCGACTTTCAGGCAGACAT
PRIMER_INTERNAL_3_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
PRIMER_LEFT_3=69,20
PRIMER_RIGHT_3=143,20
PRIMER_INTERNAL_3=91,22
PRIMER_LEFT_3_TM=60.073
PRIMER_RIGHT_3_TM=60.801
PRIMER_INTERNAL_3_TM=59.736
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=31.818
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=0.898749
PRIMER_LEFT_4_PENALTY=0.072951
PRIMER_RIGHT_4_PENALTY=0.825798
PRIMER_INTERNAL_4_PENALTY=2.263560
PRIMER_LEFT_4_SEQUENCE=CAGGCACCATTTCTCCTCAT
PRIMER_RIGHT_4_SEQUENCE=AATGTTTCCGACTTTCAGGC
PRIMER_INTERNAL_4_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA
PRIMER_LEFT_4=69,20
PRIMER_RIGHT_4=149,20
PRIMER_INTERNAL_4=91,22
PRIMER_LEFT_4_TM=60.073
PRIMER_RIGHT_4_TM=59.174
PRIMER_INTERNAL_4_TM=59.736
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=31.818
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH698
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGATGGCAGACACCTGAGGAGCCAGGTGCCTGAAATTTCCTCCACCTGCTTGAGGAGCGCCAAAGCCCAGGTGCTGGCTAGACAACCCCTTCTGGCTGCCTAAGCAGGTGGCAGAGGAAGGAAAAAAGGTCAGAGGCAGAGTNTTGAACCCTGCCTCCCAGGTGGGTGGAAGATGCCTGTCGCCAAACTAGGGCTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT
SEQUENCE_INCLUDED_REGION=36,198
P3_COMMENT=CL is GGCAGACACCTGAGGAGC
P3_COMMENT=CR is CAGGCATCTTCCACCCAC
P3_COMMENT=CM is AGGTGCCTGAAATTTCCTCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.509144
PRIMER_LEFT_0_PENALTY=0.329550
PRIMER_RIGHT_0_PENALTY=0.179594
PRIMER_INTERNAL_0_PENALTY=0.246438
PRIMER_LEFT_0_SEQUENCE=AATTTCCTCCACCTGCTTGA
PRIMER_RIGHT_0_SEQUENCE=CCTTTTTTCCTTCCTCTGCC
PRIMER_INTERNAL_0_SEQUENCE=TAGACAACCCCTTCTGGCTG
PRIMER_LEFT_0=70,20
PRIMER_RIGHT_0=165,20
PRIMER_INTERNAL_0=115,20
PRIMER_LEFT_0_TM=59.670
PRIMER_RIGHT_0_TM=60.180
PRIMER_INTERNAL_0_TM=60.246
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=0.509144
PRIMER_LEFT_1_PENALTY=0.329550
PRIMER_RIGHT_1_PENALTY=0.179594
PRIMER_INTERNAL_1_PENALTY=0.246438
PRIMER_LEFT_1_SEQUENCE=TGAAATTTCCTCCACCTGCT
PRIMER_RIGHT_1_SEQUENCE=CCTTTTTTCCTTCCTCTGCC
PRIMER_INTERNAL_1_SEQUENCE=TAGACAACCCCTTCTGGCTG
PRIMER_LEFT_1=67,20
PRIMER_RIGHT_1=165,20
PRIMER_INTERNAL_1=115,20
PRIMER_LEFT_1_TM=59.670
PRIMER_RIGHT_1_TM=60.180
PRIMER_INTERNAL_1_TM=60.246
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=0.509144
PRIMER_LEFT_2_PENALTY=0.329550
PRIMER_RIGHT_2_PENALTY=0.179594
PRIMER_INTERNAL_2_PENALTY=0.246438
PRIMER_LEFT_2_SEQUENCE=CTGAAATTTCCTCCACCTGC
PRIMER_RIGHT_2_SEQUENCE=CCTTTTTTCCTTCCTCTGCC
PRIMER_INTERNAL_2_SEQUENCE=TAGACAACCCCTTCTGGCTG
PRIMER_LEFT_2=66,20
PRIMER_RIGHT_2=165,20
PRIMER_INTERNAL_2=115,20
PRIMER_LEFT_2_TM=59.670
PRIMER_RIGHT_2_TM=60.180
PRIMER_INTERNAL_2_TM=60.246
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=0.575987
PRIMER_LEFT_3_PENALTY=0.329550
PRIMER_RIGHT_3_PENALTY=0.246438
PRIMER_INTERNAL_3_PENALTY=4.173858
PRIMER_LEFT_3_SEQUENCE=CAGGTGCCTGAAATTTCCTC
PRIMER_RIGHT_3_SEQUENCE=CAGCCAGAAGGGGTTGTCTA
PRIMER_INTERNAL_3_SEQUENCE=CTTGAGGAGCGCCAAAGC
PRIMER_LEFT_3=59,20
PRIMER_RIGHT_3=134,20
PRIMER_INTERNAL_3=85,18
PRIMER_LEFT_3_TM=59.670
PRIMER_RIGHT_3_TM=60.246
PRIMER_INTERNAL_3_TM=62.174
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=0.689335
PRIMER_LEFT_4_PENALTY=0.442898
PRIMER_RIGHT_4_PENALTY=0.246438
PRIMER_INTERNAL_4_PENALTY=4.173858
PRIMER_LEFT_4_SEQUENCE=AGGTGCCTGAAATTTCCTCC
PRIMER_RIGHT_4_SEQUENCE=CAGCCAGAAGGGGTTGTCTA
PRIMER_INTERNAL_4_SEQUENCE=CTTGAGGAGCGCCAAAGC
PRIMER_LEFT_4=60,20
PRIMER_RIGHT_4=134,20
PRIMER_INTERNAL_4=85,18
PRIMER_LEFT_4_TM=60.443
PRIMER_RIGHT_4_TM=60.246
PRIMER_INTERNAL_4_TM=62.174
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH701
SEQUENCE_TEMPLATE=NTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGTTTCATCATATGCGAAACATGGATAAAAACAACTACCTTGCCTTACCTACTTTACAGAGTNTCTCCCCACAATTCCAAGAATTAAATGAAGAAAATGTCTGTAAAAGTGTTTTGCATGCTATAATGTAAAGCATCATTGTGAGATCTGTGATGGTTATCCCAGGTCTTTATTCCAACATCCATTTTCTTATTAATTTTTATAACTTCCATTTTTATCAGTGGGCCTCTTATTCACTAGATTTATCTGATGAAAGCCATCTGGAATGCATATTTACCAGGTTA
SEQUENCE_INCLUDED_REGION=35,156
P3_COMMENT=CL is TCATCATATGCGAAACATGG
P3_COMMENT=CR is TCACAGATCTCACAATGATGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.524112
PRIMER_LEFT_0_PENALTY=1.094139
PRIMER_RIGHT_0_PENALTY=0.429973
PRIMER_INTERNAL_0_PENALTY=5.075685
PRIMER_LEFT_0_SEQUENCE=TCATCATATGCGAAACATGGA
PRIMER_RIGHT_0_SEQUENCE=TTCTTGGAATTGTGGGGAGA
PRIMER_INTERNAL_0_SEQUENCE=AAAAACAACTACCTTGCCTTACC
PRIMER_LEFT_0=41,21
PRIMER_RIGHT_0=119,20
PRIMER_INTERNAL_0=63,23
PRIMER_LEFT_0_TM=59.906
PRIMER_RIGHT_0_TM=60.430
PRIMER_INTERNAL_0_TM=57.924
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=79
PRIMER_PAIR_1_PENALTY=1.873877
PRIMER_LEFT_1_PENALTY=1.094139
PRIMER_RIGHT_1_PENALTY=0.779738
PRIMER_INTERNAL_1_PENALTY=5.075685
PRIMER_LEFT_1_SEQUENCE=TCATCATATGCGAAACATGGA
PRIMER_RIGHT_1_SEQUENCE=AATTCTTGGAATTGTGGGGA
PRIMER_INTERNAL_1_SEQUENCE=AAAAACAACTACCTTGCCTTACC
PRIMER_LEFT_1=41,21
PRIMER_RIGHT_1=121,20
PRIMER_INTERNAL_1=63,23
PRIMER_LEFT_1_TM=59.906
PRIMER_RIGHT_1_TM=59.220
PRIMER_INTERNAL_1_TM=57.924
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=81
PRIMER_PAIR_2_PENALTY=2.038891
PRIMER_LEFT_2_PENALTY=1.608918
PRIMER_RIGHT_2_PENALTY=0.429973
PRIMER_INTERNAL_2_PENALTY=5.075685
PRIMER_LEFT_2_SEQUENCE=TTCATCATATGCGAAACATGG
PRIMER_RIGHT_2_SEQUENCE=TTCTTGGAATTGTGGGGAGA
PRIMER_INTERNAL_2_SEQUENCE=AAAAACAACTACCTTGCCTTACC
PRIMER_LEFT_2=40,21
PRIMER_RIGHT_2=119,20
PRIMER_INTERNAL_2=63,23
PRIMER_LEFT_2_TM=59.391
PRIMER_RIGHT_2_TM=60.430
PRIMER_INTERNAL_2_TM=57.924
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=2.258256
PRIMER_LEFT_3_PENALTY=1.094139
PRIMER_RIGHT_3_PENALTY=1.164117
PRIMER_INTERNAL_3_PENALTY=5.075685
PRIMER_LEFT_3_SEQUENCE=TCATCATATGCGAAACATGGA
PRIMER_RIGHT_3_SEQUENCE=ATTCTTGGAATTGTGGGGAG
PRIMER_INTERNAL_3_SEQUENCE=AAAAACAACTACCTTGCCTTACC
PRIMER_LEFT_3=41,21
PRIMER_RIGHT_3=120,20
PRIMER_INTERNAL_3=63,23
PRIMER_LEFT_3_TM=59.906
PRIMER_RIGHT_3_TM=58.836
PRIMER_INTERNAL_3_TM=57.924
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=2.266978
PRIMER_LEFT_4_PENALTY=1.094139
PRIMER_RIGHT_4_PENALTY=1.172839
PRIMER_INTERNAL_4_PENALTY=5.075685
PRIMER_LEFT_4_SEQUENCE=TCATCATATGCGAAACATGGA
PRIMER_RIGHT_4_SEQUENCE=AATTCTTGGAATTGTGGGGAG
PRIMER_INTERNAL_4_SEQUENCE=AAAAACAACTACCTTGCCTTACC
PRIMER_LEFT_4=41,21
PRIMER_RIGHT_4=121,21
PRIMER_INTERNAL_4=63,23
PRIMER_LEFT_4_TM=59.906
PRIMER_RIGHT_4_TM=60.173
PRIMER_INTERNAL_4_TM=57.924
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH704
SEQUENCE_TEMPLATE=TCGACTCTAGAGGATCCCCCTAACTCTTGTNTTTCTAAGCACTTAACAATGGATAATGNAAACATTCTAGGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTTGCATTAATGAATCGGCCAACGCGCGGGGAG
SEQUENCE_INCLUDED_REGION=19,55
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH707
SEQUENCE_TEMPLATE=TATATGGATTTTTATCTTGGGTNTAATTATTAGGTTATTACAAAAGTAATTATGTTTTTTGGCATTATTTTCAAATGGCAAAAACCGCAGTTACTTTTGCAACAGCCTAATATTTAATAGCCTTGCTTCTTTCTTGGAAACTGTTCTTGTTACTTTGAGTNTCTACCAGTNCCATCTTTTCTTGTTCACGATGTAATGTCTGTTTGGACAGATGTATCAAAGTACCTTAATCTAAG
SEQUENCE_INCLUDED_REGION=0,236
P3_COMMENT=CL is TTTGGCATTATTTTCAAATGG
P3_COMMENT=CR is TGTCCAAACAGACATTACATCG
P3_COMMENT=CM is AAAAACCGCAGTTACTTTTGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.634264
PRIMER_LEFT_0_PENALTY=1.203207
PRIMER_RIGHT_0_PENALTY=0.431057
PRIMER_INTERNAL_0_PENALTY=0.088865
PRIMER_LEFT_0_SEQUENCE=GGCATTATTTTCAAATGGCAA
PRIMER_RIGHT_0_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
PRIMER_INTERNAL_0_SEQUENCE=CCGCAGTTACTTTTGCAACA
PRIMER_LEFT_0=60,21
PRIMER_RIGHT_0=138,20
PRIMER_INTERNAL_0=84,20
PRIMER_LEFT_0_TM=59.797
PRIMER_RIGHT_0_TM=59.569
PRIMER_INTERNAL_0_TM=59.911
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=79
PRIMER_PAIR_1_PENALTY=1.634264
PRIMER_LEFT_1_PENALTY=1.203207
PRIMER_RIGHT_1_PENALTY=0.431057
PRIMER_INTERNAL_1_PENALTY=0.088865
PRIMER_LEFT_1_SEQUENCE=TTGGCATTATTTTCAAATGGC
PRIMER_RIGHT_1_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
PRIMER_INTERNAL_1_SEQUENCE=CCGCAGTTACTTTTGCAACA
PRIMER_LEFT_1=58,21
PRIMER_RIGHT_1=138,20
PRIMER_INTERNAL_1=84,20
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.569
PRIMER_INTERNAL_1_TM=59.911
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=81
PRIMER_PAIR_2_PENALTY=1.943817
PRIMER_LEFT_2_PENALTY=1.512759
PRIMER_RIGHT_2_PENALTY=0.431057
PRIMER_INTERNAL_2_PENALTY=0.088865
PRIMER_LEFT_2_SEQUENCE=GGCATTATTTTCAAATGGCA
PRIMER_RIGHT_2_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
PRIMER_INTERNAL_2_SEQUENCE=CCGCAGTTACTTTTGCAACA
PRIMER_LEFT_2=60,20
PRIMER_RIGHT_2=138,20
PRIMER_INTERNAL_2=84,20
PRIMER_LEFT_2_TM=58.487
PRIMER_RIGHT_2_TM=59.569
PRIMER_INTERNAL_2_TM=59.911
PRIMER_LEFT_2_GC_PERCENT=35.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=1.943817
PRIMER_LEFT_3_PENALTY=1.512759
PRIMER_RIGHT_3_PENALTY=0.431057
PRIMER_INTERNAL_3_PENALTY=0.088865
PRIMER_LEFT_3_SEQUENCE=TGGCATTATTTTCAAATGGC
PRIMER_RIGHT_3_SEQUENCE=TTCCAAGAAAGAAGCAAGGC
PRIMER_INTERNAL_3_SEQUENCE=CCGCAGTTACTTTTGCAACA
PRIMER_LEFT_3=59,20
PRIMER_RIGHT_3=138,20
PRIMER_INTERNAL_3=84,20
PRIMER_LEFT_3_TM=58.487
PRIMER_RIGHT_3_TM=59.569
PRIMER_INTERNAL_3_TM=59.911
PRIMER_LEFT_3_GC_PERCENT=35.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=2.008682
PRIMER_LEFT_4_PENALTY=1.203207
PRIMER_RIGHT_4_PENALTY=0.805475
PRIMER_INTERNAL_4_PENALTY=0.088865
PRIMER_LEFT_4_SEQUENCE=GGCATTATTTTCAAATGGCAA
PRIMER_RIGHT_4_SEQUENCE=TCCAAGAAAGAAGCAAGGCT
PRIMER_INTERNAL_4_SEQUENCE=CCGCAGTTACTTTTGCAACA
PRIMER_LEFT_4=60,21
PRIMER_RIGHT_4=137,20
PRIMER_INTERNAL_4=84,20
PRIMER_LEFT_4_TM=59.797
PRIMER_RIGHT_4_TM=59.195
PRIMER_INTERNAL_4_TM=59.911
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH714
SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAACCTGGACAAATGACTATTGATAAAGAATTTATTTTCTGTCACCTTTAAGGAGAGCAGAGGGTGGTACAGCCTTTGTATTATTTTTNCTTTTTTAAAAAAAGTTTGTTTGAAGCCTCCTTCATGAAAACTTCAGCTGTCAAATCTTGGCAAAAAATGTGTGTTCATCTATATGATAGTTCTTAGGAGAATCAATAG
SEQUENCE_INCLUDED_REGION=35,201
P3_COMMENT=CL is TTCTGTCACCTTTAAGGAGAGC
P3_COMMENT=CR is TGAACACACATTTTTTGCCA
P3_COMMENT=CM is GAGGGTGGTACAGCCTTTGT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.532509
PRIMER_LEFT_0_PENALTY=0.520448
PRIMER_RIGHT_0_PENALTY=0.012060
PRIMER_INTERNAL_0_PENALTY=1.152564
PRIMER_LEFT_0_SEQUENCE=AGAGCAGAGGGTGGTACAGC
PRIMER_RIGHT_0_SEQUENCE=TTGCCAAGATTTGACAGCTG
PRIMER_INTERNAL_0_SEQUENCE=TGTTTGAAGCCTCCTTCATG
PRIMER_LEFT_0=91,20
PRIMER_RIGHT_0=190,20
PRIMER_INTERNAL_0=144,20
PRIMER_LEFT_0_TM=59.480
PRIMER_RIGHT_0_TM=59.988
PRIMER_INTERNAL_0_TM=58.847
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.804296
PRIMER_LEFT_1_PENALTY=0.792236
PRIMER_RIGHT_1_PENALTY=0.012060
PRIMER_INTERNAL_1_PENALTY=1.152564
PRIMER_LEFT_1_SEQUENCE=AGAGGGTGGTACAGCCTTTG
PRIMER_RIGHT_1_SEQUENCE=TTGCCAAGATTTGACAGCTG
PRIMER_INTERNAL_1_SEQUENCE=TGTTTGAAGCCTCCTTCATG
PRIMER_LEFT_1=96,20
PRIMER_RIGHT_1=190,20
PRIMER_INTERNAL_1=144,20
PRIMER_LEFT_1_TM=59.208
PRIMER_RIGHT_1_TM=59.988
PRIMER_INTERNAL_1_TM=58.847
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=0.804296
PRIMER_LEFT_2_PENALTY=0.792236
PRIMER_RIGHT_2_PENALTY=0.012060
PRIMER_INTERNAL_2_PENALTY=1.152564
PRIMER_LEFT_2_SEQUENCE=CAGAGGGTGGTACAGCCTTT
PRIMER_RIGHT_2_SEQUENCE=TTGCCAAGATTTGACAGCTG
PRIMER_INTERNAL_2_SEQUENCE=TGTTTGAAGCCTCCTTCATG
PRIMER_LEFT_2=95,20
PRIMER_RIGHT_2=190,20
PRIMER_INTERNAL_2=144,20
PRIMER_LEFT_2_TM=59.208
PRIMER_RIGHT_2_TM=59.988
PRIMER_INTERNAL_2_TM=58.847
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.946936
PRIMER_LEFT_3_PENALTY=0.934876
PRIMER_RIGHT_3_PENALTY=0.012060
PRIMER_INTERNAL_3_PENALTY=1.152564
PRIMER_LEFT_3_SEQUENCE=GAGGGTGGTACAGCCTTTGT
PRIMER_RIGHT_3_SEQUENCE=TTGCCAAGATTTGACAGCTG
PRIMER_INTERNAL_3_SEQUENCE=TGTTTGAAGCCTCCTTCATG
PRIMER_LEFT_3=97,20
PRIMER_RIGHT_3=190,20
PRIMER_INTERNAL_3=144,20
PRIMER_LEFT_3_TM=59.065
PRIMER_RIGHT_3_TM=59.988
PRIMER_INTERNAL_3_TM=58.847
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=1.057493
PRIMER_LEFT_4_PENALTY=1.045432
PRIMER_RIGHT_4_PENALTY=0.012060
PRIMER_INTERNAL_4_PENALTY=1.152564
PRIMER_LEFT_4_SEQUENCE=AGAGGGTGGTACAGCCTTTGT
PRIMER_RIGHT_4_SEQUENCE=TTGCCAAGATTTGACAGCTG
PRIMER_INTERNAL_4_SEQUENCE=TGTTTGAAGCCTCCTTCATG
PRIMER_LEFT_4=96,21
PRIMER_RIGHT_4=190,20
PRIMER_INTERNAL_4=144,20
PRIMER_LEFT_4_TM=60.045
PRIMER_RIGHT_4_TM=59.988
PRIMER_INTERNAL_4_TM=58.847
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH715
SEQUENCE_TEMPLATE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCTTCCTGAGAACAAAGGGCTCAGGCTATGATGTGGGGAGGAGGCCTGGACCTGTGTCCTATGCCTGGGNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
SEQUENCE_INCLUDED_REGION=37,76
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH718
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTCTTTGGCTATCTCATTCTCACACCTCCCTCATCACCTTTTTCTTGGGCACGTGCATTTCTTTTTCTCCTAGGGTTGAGCACAGATCTAACTTGAATAATTAAAGGAAGCTTTGTGTAATGAAAGCCAAAGCAGAAAGGAACAAAAGACTTTTAGCATGTAATAGAAGGGAATAGTGAACATGGAGGCAGAGGGAGTCATTTTTGGGGTGTAAGAGTCGATTATCTTTCTTGTCCCCTGGGCCGTTCGTTAGGGTGGCTTTACTTTGTTAATCCTGCACTT
SEQUENCE_INCLUDED_REGION=36,209
P3_COMMENT=CL is TGGCTATCTCATTCTCACACC
P3_COMMENT=CR is AAAAATGACTCCCTCTGCCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.336494
PRIMER_LEFT_0_PENALTY=0.006945
PRIMER_RIGHT_0_PENALTY=0.329550
PRIMER_INTERNAL_0_PENALTY=4.331778
PRIMER_LEFT_0_SEQUENCE=AGCCAAAGCAGAAAGGAACA
PRIMER_RIGHT_0_SEQUENCE=CAAAAATGACTCCCTCTGCC
PRIMER_INTERNAL_0_SEQUENCE=AGAAGGGAATAGTGAACATGGA
PRIMER_LEFT_0=162,20
PRIMER_RIGHT_0=243,20
PRIMER_INTERNAL_0=202,22
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=59.670
PRIMER_INTERNAL_0_TM=57.668
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=0.336494
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.329550
PRIMER_INTERNAL_1_PENALTY=4.331778
PRIMER_LEFT_1_SEQUENCE=AGCCAAAGCAGAAAGGAACA
PRIMER_RIGHT_1_SEQUENCE=CCAAAAATGACTCCCTCTGC
PRIMER_INTERNAL_1_SEQUENCE=GAAGGGAATAGTGAACATGGAG
PRIMER_LEFT_1=162,20
PRIMER_RIGHT_1=244,20
PRIMER_INTERNAL_1=203,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.670
PRIMER_INTERNAL_1_TM=57.668
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=0.602767
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=0.595822
PRIMER_INTERNAL_2_PENALTY=7.082413
PRIMER_LEFT_2_SEQUENCE=AGCCAAAGCAGAAAGGAACA
PRIMER_RIGHT_2_SEQUENCE=AAATGACTCCCTCTGCCTCC
PRIMER_INTERNAL_2_SEQUENCE=GCATGTAATAGAAGGGAATAGTGAA
PRIMER_LEFT_2=162,20
PRIMER_RIGHT_2=240,20
PRIMER_INTERNAL_2=193,25
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=60.596
PRIMER_INTERNAL_2_TM=57.918
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=36.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=0.633316
PRIMER_LEFT_3_PENALTY=0.626371
PRIMER_RIGHT_3_PENALTY=0.006945
PRIMER_INTERNAL_3_PENALTY=1.701644
PRIMER_LEFT_3_SEQUENCE=GGCACGTGCATTTCTTTTTC
PRIMER_RIGHT_3_SEQUENCE=TGTTCCTTTCTGCTTTGGCT
PRIMER_INTERNAL_3_SEQUENCE=TCCTAGGGTTGAGCACAGATC
PRIMER_LEFT_3=85,20
PRIMER_RIGHT_3=181,20
PRIMER_INTERNAL_3=105,21
PRIMER_LEFT_3_TM=60.626
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=59.298
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=0.694720
PRIMER_LEFT_4_PENALTY=0.365170
PRIMER_RIGHT_4_PENALTY=0.329550
PRIMER_INTERNAL_4_PENALTY=4.331778
PRIMER_LEFT_4_SEQUENCE=GCCAAAGCAGAAAGGAACAA
PRIMER_RIGHT_4_SEQUENCE=CAAAAATGACTCCCTCTGCC
PRIMER_INTERNAL_4_SEQUENCE=AGAAGGGAATAGTGAACATGGA
PRIMER_LEFT_4=163,20
PRIMER_RIGHT_4=243,20
PRIMER_INTERNAL_4=202,22
PRIMER_LEFT_4_TM=60.365
PRIMER_RIGHT_4_TM=59.670
PRIMER_INTERNAL_4_TM=57.668
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH719
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAAGTNTCTTAAATCTTTGCTTCAAAGAAGTTCTTGCTCAGGAAGTTATTCATTCAGCAACCTAAAATTGTTTTTTGAGTACATATCAAGCACAGGGTTCTGAGCAATGTCTTAGGAAGACCATAAAGGTGAATAAATGAGTGTTTCTACCCTGAGGAATTTATCAAAGATGTTAAGTTATCTCCTTAGAGGTATAAGTCATATAGGCATATTCTATGTATACTAAAGGTGGTATGGCATAAGAGTACATATAGTCACCAACTTANGAATTGTTTGACTTGGNCGGGCGCAGTGGCTCACG
SEQUENCE_INCLUDED_REGION=36,303
P3_COMMENT=CL is TTGCTTCAAAGAAGTTCTTGC
P3_COMMENT=CR is TATGTACTCTTATGCCATACCACC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.887466
PRIMER_LEFT_0_PENALTY=2.761640
PRIMER_RIGHT_0_PENALTY=0.125826
PRIMER_INTERNAL_0_PENALTY=4.392765
PRIMER_LEFT_0_SEQUENCE=TCAAAGAAGTTCTTGCTCAGGA
PRIMER_RIGHT_0_SEQUENCE=ATTGCTCAGAACCCTGTGCT
PRIMER_INTERNAL_0_SEQUENCE=CAGCAACCTAAAATTGTTTTTTGA
PRIMER_LEFT_0=59,22
PRIMER_RIGHT_0=145,20
PRIMER_INTERNAL_0=92,24
PRIMER_LEFT_0_TM=59.238
PRIMER_RIGHT_0_TM=59.874
PRIMER_INTERNAL_0_TM=59.607
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=3.219621
PRIMER_LEFT_1_PENALTY=3.093794
PRIMER_RIGHT_1_PENALTY=0.125826
PRIMER_INTERNAL_1_PENALTY=4.253252
PRIMER_LEFT_1_SEQUENCE=GCTTCAAAGAAGTTCTTGCTCA
PRIMER_RIGHT_1_SEQUENCE=ATTGCTCAGAACCCTGTGCT
PRIMER_INTERNAL_1_SEQUENCE=GGAAGTTATTCATTCAGCAACC
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=145,20
PRIMER_INTERNAL_1=78,22
PRIMER_LEFT_1_TM=58.906
PRIMER_RIGHT_1_TM=59.874
PRIMER_INTERNAL_1_TM=57.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=9.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=3.219621
PRIMER_LEFT_2_PENALTY=3.093794
PRIMER_RIGHT_2_PENALTY=0.125826
PRIMER_INTERNAL_2_PENALTY=4.253252
PRIMER_LEFT_2_SEQUENCE=TGCTTCAAAGAAGTTCTTGCTC
PRIMER_RIGHT_2_SEQUENCE=ATTGCTCAGAACCCTGTGCT
PRIMER_INTERNAL_2_SEQUENCE=GGAAGTTATTCATTCAGCAACC
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=145,20
PRIMER_INTERNAL_2=78,22
PRIMER_LEFT_2_TM=58.906
PRIMER_RIGHT_2_TM=59.874
PRIMER_INTERNAL_2_TM=57.747
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_3_PENALTY=3.292973
PRIMER_LEFT_3_PENALTY=3.167146
PRIMER_RIGHT_3_PENALTY=0.125826
PRIMER_INTERNAL_3_PENALTY=4.392765
PRIMER_LEFT_3_SEQUENCE=GCTTCAAAGAAGTTCTTGCTCAG
PRIMER_RIGHT_3_SEQUENCE=ATTGCTCAGAACCCTGTGCT
PRIMER_INTERNAL_3_SEQUENCE=CAGCAACCTAAAATTGTTTTTTGA
PRIMER_LEFT_3=56,23
PRIMER_RIGHT_3=145,20
PRIMER_INTERNAL_3=92,24
PRIMER_LEFT_3_TM=59.833
PRIMER_RIGHT_3_TM=59.874
PRIMER_INTERNAL_3_TM=59.607
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY=9.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=3.301463
PRIMER_LEFT_4_PENALTY=3.175636
PRIMER_RIGHT_4_PENALTY=0.125826
PRIMER_INTERNAL_4_PENALTY=4.253252
PRIMER_LEFT_4_SEQUENCE=TTGCTTCAAAGAAGTTCTTGCTC
PRIMER_RIGHT_4_SEQUENCE=ATTGCTCAGAACCCTGTGCT
PRIMER_INTERNAL_4_SEQUENCE=GGAAGTTATTCATTCAGCAACC
PRIMER_LEFT_4=54,23
PRIMER_RIGHT_4=145,20
PRIMER_INTERNAL_4=78,22
PRIMER_LEFT_4_TM=60.176
PRIMER_RIGHT_4_TM=59.874
PRIMER_INTERNAL_4_TM=57.747
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=9.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH720
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCCAAAGAGCNTGCTTTTTAATTAGTNCAACATGCAGCAGCAAATNAGGGAAATGGGGAAGAAATTATTTGTCATCAAATTGAAATACATGTAAAGAAAACACAAGCAAAAATGTATTAAAGCCTTTTATGAGGAGGCTTTTAGTCAACATTTGGCTTGGTTAAAACCTTGAGCCAAATGTTTATTAAGGCACAGCAGTAAAGGAAGCAAAANCCATTTTTTGTACCTTT
SEQUENCE_INCLUDED_REGION=36,166
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.346210
PRIMER_LEFT_0_PENALTY=1.245238
PRIMER_RIGHT_0_PENALTY=2.100972
PRIMER_INTERNAL_0_PENALTY=5.346517
PRIMER_LEFT_0_SEQUENCE=AGGGAAATGGGGAAGAAATT
PRIMER_RIGHT_0_SEQUENCE=AAAAGCCTCCTCATAAAAGGC
PRIMER_INTERNAL_0_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
PRIMER_LEFT_0=83,20
PRIMER_RIGHT_0=178,21
PRIMER_INTERNAL_0=126,25
PRIMER_LEFT_0_TM=58.755
PRIMER_RIGHT_0_TM=58.899
PRIMER_INTERNAL_0_TM=59.653
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_INTERNAL_0_GC_PERCENT=28.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=3.473354
PRIMER_LEFT_1_PENALTY=1.245238
PRIMER_RIGHT_1_PENALTY=2.228115
PRIMER_INTERNAL_1_PENALTY=5.346517
PRIMER_LEFT_1_SEQUENCE=AGGGAAATGGGGAAGAAATT
PRIMER_RIGHT_1_SEQUENCE=AAGCCTCCTCATAAAAGGCTTT
PRIMER_INTERNAL_1_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
PRIMER_LEFT_1=83,20
PRIMER_RIGHT_1=176,22
PRIMER_INTERNAL_1=126,25
PRIMER_LEFT_1_TM=58.755
PRIMER_RIGHT_1_TM=59.772
PRIMER_INTERNAL_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_INTERNAL_1_GC_PERCENT=28.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=3.473354
PRIMER_LEFT_2_PENALTY=1.245238
PRIMER_RIGHT_2_PENALTY=2.228115
PRIMER_INTERNAL_2_PENALTY=5.346517
PRIMER_LEFT_2_SEQUENCE=AGGGAAATGGGGAAGAAATT
PRIMER_RIGHT_2_SEQUENCE=AAAGCCTCCTCATAAAAGGCTT
PRIMER_INTERNAL_2_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
PRIMER_LEFT_2=83,20
PRIMER_RIGHT_2=177,22
PRIMER_INTERNAL_2=126,25
PRIMER_LEFT_2_TM=58.755
PRIMER_RIGHT_2_TM=59.772
PRIMER_INTERNAL_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_INTERNAL_2_GC_PERCENT=28.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=8.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=3.473354
PRIMER_LEFT_3_PENALTY=1.245238
PRIMER_RIGHT_3_PENALTY=2.228115
PRIMER_INTERNAL_3_PENALTY=5.346517
PRIMER_LEFT_3_SEQUENCE=AGGGAAATGGGGAAGAAATT
PRIMER_RIGHT_3_SEQUENCE=AAAAGCCTCCTCATAAAAGGCT
PRIMER_INTERNAL_3_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
PRIMER_LEFT_3=83,20
PRIMER_RIGHT_3=178,22
PRIMER_INTERNAL_3=126,25
PRIMER_LEFT_3_TM=58.755
PRIMER_RIGHT_3_TM=59.772
PRIMER_INTERNAL_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_INTERNAL_3_GC_PERCENT=28.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=3.666072
PRIMER_LEFT_4_PENALTY=1.245238
PRIMER_RIGHT_4_PENALTY=2.420834
PRIMER_INTERNAL_4_PENALTY=5.346517
PRIMER_LEFT_4_SEQUENCE=AGGGAAATGGGGAAGAAATT
PRIMER_RIGHT_4_SEQUENCE=GCCTCCTCATAAAAGGCTTT
PRIMER_INTERNAL_4_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG
PRIMER_LEFT_4=83,20
PRIMER_RIGHT_4=174,20
PRIMER_INTERNAL_4=126,25
PRIMER_LEFT_4_TM=58.755
PRIMER_RIGHT_4_TM=57.579
PRIMER_INTERNAL_4_TM=59.653
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=28.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=7.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH721
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATTGGGTNTCTCTCTCCCTACTGTGTAGAAGACCCTTACTTAATTGCTACTTCCTTAATGATGCCTTTCCCAGGACAAAACTCCATGGTGACTTTGTGTTGCTCTGATAGCCCTTCTCATGGGTGGTAACTCTGTCATTTGTTTTCCATCTGTCCATCCATTCCACAGACAGTGATTACAACCAGGATTGCTGCTGGGCTGTGAAAATACTAAAAACAATGAGGCAATGTGTCTACCTTCAAGAGGTTTACAGTCTATAATCTGTGCTATACTGTAATGATTTCTGTGACTGCGTTTGATATTTCTAG
SEQUENCE_INCLUDED_REGION=36,311
P3_COMMENT=CL is AGGACAAAACTCCATGGTGA
P3_COMMENT=CR is AAACGCAGTCACAGAAATCA
P3_COMMENT=CM is GTGTTGCTCTGATAGCCCTT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.488969
PRIMER_LEFT_0_PENALTY=0.353069
PRIMER_RIGHT_0_PENALTY=0.135900
PRIMER_INTERNAL_0_PENALTY=0.527169
PRIMER_LEFT_0_SEQUENCE=CTGATAGCCCTTCTCATGGG
PRIMER_RIGHT_0_SEQUENCE=CAGCAGCAATCCTGGTTGTA
PRIMER_INTERNAL_0_SEQUENCE=TCCATCCATTCCACAGACAG
PRIMER_LEFT_0=142,20
PRIMER_RIGHT_0=234,20
PRIMER_INTERNAL_0=191,20
PRIMER_LEFT_0_TM=59.647
PRIMER_RIGHT_0_TM=59.864
PRIMER_INTERNAL_0_TM=59.473
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=93
PRIMER_PAIR_1_PENALTY=0.535385
PRIMER_LEFT_1_PENALTY=0.008216
PRIMER_RIGHT_1_PENALTY=0.527169
PRIMER_INTERNAL_1_PENALTY=0.353069
PRIMER_LEFT_1_SEQUENCE=CCATGGTGACTTTGTGTTGC
PRIMER_RIGHT_1_SEQUENCE=CTGTGGAATGGATGGACAGA
PRIMER_INTERNAL_1_SEQUENCE=CTGATAGCCCTTCTCATGGG
PRIMER_LEFT_1=121,20
PRIMER_RIGHT_1=206,20
PRIMER_INTERNAL_1=142,20
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.473
PRIMER_INTERNAL_1_TM=59.647
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=86
PRIMER_PAIR_2_PENALTY=0.535385
PRIMER_LEFT_2_PENALTY=0.008216
PRIMER_RIGHT_2_PENALTY=0.527169
PRIMER_INTERNAL_2_PENALTY=0.353069
PRIMER_LEFT_2_SEQUENCE=CCATGGTGACTTTGTGTTGC
PRIMER_RIGHT_2_SEQUENCE=TCTGTGGAATGGATGGACAG
PRIMER_INTERNAL_2_SEQUENCE=CTGATAGCCCTTCTCATGGG
PRIMER_LEFT_2=121,20
PRIMER_RIGHT_2=207,20
PRIMER_INTERNAL_2=142,20
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.473
PRIMER_INTERNAL_2_TM=59.647
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=0.535385
PRIMER_LEFT_3_PENALTY=0.008216
PRIMER_RIGHT_3_PENALTY=0.527169
PRIMER_INTERNAL_3_PENALTY=0.353069
PRIMER_LEFT_3_SEQUENCE=CCATGGTGACTTTGTGTTGC
PRIMER_RIGHT_3_SEQUENCE=CTGTCTGTGGAATGGATGGA
PRIMER_INTERNAL_3_SEQUENCE=CTGATAGCCCTTCTCATGGG
PRIMER_LEFT_3=121,20
PRIMER_RIGHT_3=210,20
PRIMER_INTERNAL_3=142,20
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.473
PRIMER_INTERNAL_3_TM=59.647
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=0.628382
PRIMER_LEFT_4_PENALTY=0.492482
PRIMER_RIGHT_4_PENALTY=0.135900
PRIMER_INTERNAL_4_PENALTY=0.527169
PRIMER_LEFT_4_SEQUENCE=GATAGCCCTTCTCATGGGTG
PRIMER_RIGHT_4_SEQUENCE=CAGCAGCAATCCTGGTTGTA
PRIMER_INTERNAL_4_SEQUENCE=TCCATCCATTCCACAGACAG
PRIMER_LEFT_4=144,20
PRIMER_RIGHT_4=234,20
PRIMER_INTERNAL_4=191,20
PRIMER_LEFT_4_TM=59.508
PRIMER_RIGHT_4_TM=59.864
PRIMER_INTERNAL_4_TM=59.473
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH724
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTGCAGANCTGGGGTTCTGATGGTTAGGATGAGCCACTTGGNNATAGATGATAGGTGAGCCATTCTAAAATCATAAAAACAAAAATCAATTCAATGAACTTAGTGAATTCATCTTACTCAGTCTGTCTTATTAAGTCTCAATGCCCCCAGGTCCTTTGCAAAAACAATGCTCATTTTGACTGCACCCTCCAGAAAAAAAGTTGCAGGAGAACTTTTATTTTGTTTCCAAGCCCACTGAAGGTAAGTTTTCTGTTTTGTATCTCAATACCTTTAACCCAAAAAGGAGTTCCCATTTCATTCCTCCTACATG
SEQUENCE_INCLUDED_REGION=36,311
P3_COMMENT=CL is CTGGGGTTCTGATGGTTAGG
P3_COMMENT=CR is TGTAGGAGGAATGAAATGGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.534534
PRIMER_LEFT_0_PENALTY=0.262854
PRIMER_RIGHT_0_PENALTY=0.271680
PRIMER_INTERNAL_0_PENALTY=1.995898
PRIMER_LEFT_0_SEQUENCE=GCTCATTTTGACTGCACCCT
PRIMER_RIGHT_0_SEQUENCE=CTTACCTTCAGTGGGCTTGG
PRIMER_INTERNAL_0_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG
PRIMER_LEFT_0=205,20
PRIMER_RIGHT_0=281,20
PRIMER_INTERNAL_0=225,21
PRIMER_LEFT_0_TM=60.263
PRIMER_RIGHT_0_TM=59.728
PRIMER_INTERNAL_0_TM=59.004
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=0.889270
PRIMER_LEFT_1_PENALTY=0.262854
PRIMER_RIGHT_1_PENALTY=0.626415
PRIMER_INTERNAL_1_PENALTY=1.995898
PRIMER_LEFT_1_SEQUENCE=GCTCATTTTGACTGCACCCT
PRIMER_RIGHT_1_SEQUENCE=TACCTTCAGTGGGCTTGGAA
PRIMER_INTERNAL_1_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG
PRIMER_LEFT_1=205,20
PRIMER_RIGHT_1=279,20
PRIMER_INTERNAL_1=225,21
PRIMER_LEFT_1_TM=60.263
PRIMER_RIGHT_1_TM=60.626
PRIMER_INTERNAL_1_TM=59.004
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=0.889270
PRIMER_LEFT_2_PENALTY=0.262854
PRIMER_RIGHT_2_PENALTY=0.626415
PRIMER_INTERNAL_2_PENALTY=1.995898
PRIMER_LEFT_2_SEQUENCE=GCTCATTTTGACTGCACCCT
PRIMER_RIGHT_2_SEQUENCE=TTACCTTCAGTGGGCTTGGA
PRIMER_INTERNAL_2_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG
PRIMER_LEFT_2=205,20
PRIMER_RIGHT_2=280,20
PRIMER_INTERNAL_2=225,21
PRIMER_LEFT_2_TM=60.263
PRIMER_RIGHT_2_TM=60.626
PRIMER_INTERNAL_2_TM=59.004
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=1.188227
PRIMER_LEFT_3_PENALTY=1.029480
PRIMER_RIGHT_3_PENALTY=0.158747
PRIMER_INTERNAL_3_PENALTY=8.725741
PRIMER_LEFT_3_SEQUENCE=TTTTGTTTCCAAGCCCACTG
PRIMER_RIGHT_3_SEQUENCE=AATGGGAACTCCTTTTTGGG
PRIMER_INTERNAL_3_SEQUENCE=GGTAAGTTTTCTGTTTTGTATCTCAA
PRIMER_LEFT_3=254,20
PRIMER_RIGHT_3=330,20
PRIMER_INTERNAL_3=276,26
PRIMER_LEFT_3_TM=61.029
PRIMER_RIGHT_3_TM=60.159
PRIMER_INTERNAL_3_TM=57.274
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=1.219518
PRIMER_LEFT_4_PENALTY=0.066323
PRIMER_RIGHT_4_PENALTY=1.153194
PRIMER_INTERNAL_4_PENALTY=1.132549
PRIMER_LEFT_4_SEQUENCE=TTAAGTCTCAATGCCCCCAG
PRIMER_RIGHT_4_SEQUENCE=TCCTGCAACTTTTTTTCTGGA
PRIMER_INTERNAL_4_SEQUENCE=AATGCTCATTTTGACTGCACC
PRIMER_LEFT_4=167,20
PRIMER_RIGHT_4=244,21
PRIMER_INTERNAL_4=202,21
PRIMER_LEFT_4_TM=60.066
PRIMER_RIGHT_4_TM=59.847
PRIMER_INTERNAL_4_TM=60.133
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=38.095
PRIMER_INTERNAL_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH730
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGGAACCCTATAACTGATGTGTGCTGTTTTGAGAACAAAATACCTTCTCTTTGGAGTTCTGGCTTTAGAGGANATTGATAGAACTAAAAGTACATTTGACATGTTCATTACTCAGGGAAATTAATCCATTATGGCAATTAGTGCACTCTTTTTGCACACTGAGGTCTAAATACCAACTGAAAGAAGTTATAAAGATGTGATGTTAATGAGTNTTGTGAGGTTGTCTTTTCTCAAAGAAAACTGAGTACATGAATTTAATAAAAATATTCAG
SEQUENCE_INCLUDED_REGION=35,175
P3_COMMENT=CL is ATGTGTGCTGTTTTGAGAACA
P3_COMMENT=CR is TCAGTGTGCAAAAAGAGTGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.480898
PRIMER_LEFT_0_PENALTY=1.367716
PRIMER_RIGHT_0_PENALTY=2.113182
PRIMER_INTERNAL_0_PENALTY=8.777675
PRIMER_LEFT_0_SEQUENCE=CCTTCTCTTTGGAGTTCTGGC
PRIMER_RIGHT_0_SEQUENCE=TGCCATAATGGATTAATTTCCC
PRIMER_INTERNAL_0_SEQUENCE=AAGTACATTTGACATGTTCATTACTCA
PRIMER_LEFT_0=82,21
PRIMER_RIGHT_0=175,22
PRIMER_INTERNAL_0=127,27
PRIMER_LEFT_0_TM=60.368
PRIMER_RIGHT_0_TM=59.887
PRIMER_INTERNAL_0_TM=58.222
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=36.364
PRIMER_INTERNAL_0_GC_PERCENT=29.630
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=3.894783
PRIMER_LEFT_1_PENALTY=1.937220
PRIMER_RIGHT_1_PENALTY=1.957563
PRIMER_INTERNAL_1_PENALTY=3.515201
PRIMER_LEFT_1_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA
PRIMER_RIGHT_1_SEQUENCE=TGCACTAATTGCCATAATGGA
PRIMER_INTERNAL_1_SEQUENCE=TGACATGTTCATTACTCAGGGAA
PRIMER_LEFT_1=91,21
PRIMER_RIGHT_1=184,21
PRIMER_INTERNAL_1=136,23
PRIMER_LEFT_1_TM=59.063
PRIMER_RIGHT_1_TM=59.042
PRIMER_INTERNAL_1_TM=59.485
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=38.095
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=4.050402
PRIMER_LEFT_2_PENALTY=1.937220
PRIMER_RIGHT_2_PENALTY=2.113182
PRIMER_INTERNAL_2_PENALTY=8.777675
PRIMER_LEFT_2_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA
PRIMER_RIGHT_2_SEQUENCE=TGCCATAATGGATTAATTTCCC
PRIMER_INTERNAL_2_SEQUENCE=AAGTACATTTGACATGTTCATTACTCA
PRIMER_LEFT_2=91,21
PRIMER_RIGHT_2=175,22
PRIMER_INTERNAL_2=127,27
PRIMER_LEFT_2_TM=59.063
PRIMER_RIGHT_2_TM=59.887
PRIMER_INTERNAL_2_TM=58.222
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=36.364
PRIMER_INTERNAL_2_GC_PERCENT=29.630
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=4.071366
PRIMER_LEFT_3_PENALTY=2.113803
PRIMER_RIGHT_3_PENALTY=1.957563
PRIMER_INTERNAL_3_PENALTY=3.515201
PRIMER_LEFT_3_SEQUENCE=TTTGGAGTTCTGGCTTTAGAGG
PRIMER_RIGHT_3_SEQUENCE=TGCACTAATTGCCATAATGGA
PRIMER_INTERNAL_3_SEQUENCE=TGACATGTTCATTACTCAGGGAA
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=184,21
PRIMER_INTERNAL_3=136,23
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=59.042
PRIMER_INTERNAL_3_TM=59.485
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=38.095
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=4.077829
PRIMER_LEFT_4_PENALTY=1.937220
PRIMER_RIGHT_4_PENALTY=2.140609
PRIMER_INTERNAL_4_PENALTY=3.515201
PRIMER_LEFT_4_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA
PRIMER_RIGHT_4_SEQUENCE=GTGCACTAATTGCCATAATGGA
PRIMER_INTERNAL_4_SEQUENCE=TGACATGTTCATTACTCAGGGAA
PRIMER_LEFT_4=91,21
PRIMER_RIGHT_4=185,22
PRIMER_INTERNAL_4=136,23
PRIMER_LEFT_4_TM=59.063
PRIMER_RIGHT_4_TM=59.859
PRIMER_INTERNAL_4_TM=59.485
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH733
SEQUENCE_TEMPLATE=TTTTTACTTGTTTTCAGTATTCTTTAAGCCAGGAAAGTATGCCTAGTCTCAGAATTGTTTAACATAAAGACACAGAATTACGTTGGTTTCAATCCTCATGACATCGAGGGAAAAAAACCCAAATTAATATCTCAGAAAGTTATCCTGCCACTGAAGTATTAGCAAAAGTCCTTCCCATTCCATCTAGCCTTTCCATAAATGAAACTTCTACCACTTTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACG
SEQUENCE_INCLUDED_REGION=0,220
P3_COMMENT=CL is TTTTCAGTATTCTTTAAGCCAGG
P3_COMMENT=CR is AAAGTGGTAGAAGTTTCATTTATGG
P3_COMMENT=CM is ATGCCTAGTCTCAGAATTGTTTAAC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.210551
PRIMER_LEFT_0_PENALTY=0.113696
PRIMER_RIGHT_0_PENALTY=0.096855
PRIMER_INTERNAL_0_PENALTY=2.621889
PRIMER_LEFT_0_SEQUENCE=AATCCTCATGACATCGAGGG
PRIMER_RIGHT_0_SEQUENCE=TGGAATGGGAAGGACTTTTG
PRIMER_INTERNAL_0_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
PRIMER_LEFT_0=90,20
PRIMER_RIGHT_0=181,20
PRIMER_INTERNAL_0=132,22
PRIMER_LEFT_0_TM=59.886
PRIMER_RIGHT_0_TM=59.903
PRIMER_INTERNAL_0_TM=59.378
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=9.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=0.253412
PRIMER_LEFT_1_PENALTY=0.156557
PRIMER_RIGHT_1_PENALTY=0.096855
PRIMER_INTERNAL_1_PENALTY=2.621889
PRIMER_LEFT_1_SEQUENCE=CGAGGGAAAAAAACCCAAAT
PRIMER_RIGHT_1_SEQUENCE=TGGAATGGGAAGGACTTTTG
PRIMER_INTERNAL_1_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
PRIMER_LEFT_1=104,20
PRIMER_RIGHT_1=181,20
PRIMER_INTERNAL_1=132,22
PRIMER_LEFT_1_TM=60.157
PRIMER_RIGHT_1_TM=59.903
PRIMER_INTERNAL_1_TM=59.378
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=0.262897
PRIMER_LEFT_2_PENALTY=0.166042
PRIMER_RIGHT_2_PENALTY=0.096855
PRIMER_INTERNAL_2_PENALTY=2.621889
PRIMER_LEFT_2_SEQUENCE=ACATCGAGGGAAAAAAACCC
PRIMER_RIGHT_2_SEQUENCE=TGGAATGGGAAGGACTTTTG
PRIMER_INTERNAL_2_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
PRIMER_LEFT_2=100,20
PRIMER_RIGHT_2=181,20
PRIMER_INTERNAL_2=132,22
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=59.903
PRIMER_INTERNAL_2_TM=59.378
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=0.535576
PRIMER_LEFT_3_PENALTY=0.438721
PRIMER_RIGHT_3_PENALTY=0.096855
PRIMER_INTERNAL_3_PENALTY=2.621889
PRIMER_LEFT_3_SEQUENCE=ATCCTCATGACATCGAGGGA
PRIMER_RIGHT_3_SEQUENCE=TGGAATGGGAAGGACTTTTG
PRIMER_INTERNAL_3_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
PRIMER_LEFT_3=91,20
PRIMER_RIGHT_3=181,20
PRIMER_INTERNAL_3=132,22
PRIMER_LEFT_3_TM=60.439
PRIMER_RIGHT_3_TM=59.903
PRIMER_INTERNAL_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=9.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=7.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=0.740946
PRIMER_LEFT_4_PENALTY=0.644091
PRIMER_RIGHT_4_PENALTY=0.096855
PRIMER_INTERNAL_4_PENALTY=2.621889
PRIMER_LEFT_4_SEQUENCE=ATCGAGGGAAAAAAACCCAA
PRIMER_RIGHT_4_SEQUENCE=TGGAATGGGAAGGACTTTTG
PRIMER_INTERNAL_4_SEQUENCE=CAGAAAGTTATCCTGCCACTGA
PRIMER_LEFT_4=102,20
PRIMER_RIGHT_4=181,20
PRIMER_INTERNAL_4=132,22
PRIMER_LEFT_4_TM=60.644
PRIMER_RIGHT_4_TM=59.903
PRIMER_INTERNAL_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH736
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCCTTGACATCAACATCAGAAGTNAGGGACTTACCCTAACATCCTTGGCTTTAATAATCCATGACGGAGGTTTCATTCTTTAATCTAAACTTTTGTGGAAGNTATTCATAGTTTNTTCTCTACCGCNCTTGTAGNGATAATTAAATTGTACACATTGATTACCACTTTGCATTCAGTCATACTTTCTTTTAAAATAATAGTAACTAACATTTACATAGTGATTACCATATGCTATGCATTGTGCTAAGCACTTTACATTTCTTAAATCATTTAATATTCAGGAC
SEQUENCE_INCLUDED_REGION=36,286
P3_COMMENT=CL is TGGCTTTAATAATCCATGACG
P3_COMMENT=CR is GCTTAGCACAATGCATAGCA
P3_COMMENT=CM is ATTGTACACATTGATTACCACTTTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.178522
PRIMER_LEFT_0_PENALTY=2.861164
PRIMER_RIGHT_0_PENALTY=0.317359
PRIMER_INTERNAL_0_PENALTY=2.331778
PRIMER_LEFT_0_SEQUENCE=TGTCCTTGACATCAACATCAGA
PRIMER_RIGHT_0_SEQUENCE=TGAAACCTCCGTCATGGATT
PRIMER_INTERNAL_0_SEQUENCE=ACCCTAACATCCTTGGCTTT
PRIMER_LEFT_0=36,22
PRIMER_RIGHT_0=112,20
PRIMER_INTERNAL_0=70,20
PRIMER_LEFT_0_TM=59.139
PRIMER_RIGHT_0_TM=60.317
PRIMER_INTERNAL_0_TM=57.668
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=3.178522
PRIMER_LEFT_1_PENALTY=2.861164
PRIMER_RIGHT_1_PENALTY=0.317359
PRIMER_INTERNAL_1_PENALTY=2.331778
PRIMER_LEFT_1_SEQUENCE=TGTCCTTGACATCAACATCAGA
PRIMER_RIGHT_1_SEQUENCE=AATGAAACCTCCGTCATGGA
PRIMER_INTERNAL_1_SEQUENCE=ACCCTAACATCCTTGGCTTT
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=114,20
PRIMER_INTERNAL_1=70,20
PRIMER_LEFT_1_TM=59.139
PRIMER_RIGHT_1_TM=60.317
PRIMER_INTERNAL_1_TM=57.668
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=3.178522
PRIMER_LEFT_2_PENALTY=2.861164
PRIMER_RIGHT_2_PENALTY=0.317359
PRIMER_INTERNAL_2_PENALTY=2.331778
PRIMER_LEFT_2_SEQUENCE=TGTCCTTGACATCAACATCAGA
PRIMER_RIGHT_2_SEQUENCE=GAATGAAACCTCCGTCATGG
PRIMER_INTERNAL_2_SEQUENCE=ACCCTAACATCCTTGGCTTT
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=115,20
PRIMER_INTERNAL_2=70,20
PRIMER_LEFT_2_TM=59.139
PRIMER_RIGHT_2_TM=60.317
PRIMER_INTERNAL_2_TM=57.668
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=3.643566
PRIMER_LEFT_3_PENALTY=2.861164
PRIMER_RIGHT_3_PENALTY=0.782403
PRIMER_INTERNAL_3_PENALTY=2.331778
PRIMER_LEFT_3_SEQUENCE=TGTCCTTGACATCAACATCAGA
PRIMER_RIGHT_3_SEQUENCE=ATGAAACCTCCGTCATGGAT
PRIMER_INTERNAL_3_SEQUENCE=ACCCTAACATCCTTGGCTTT
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=113,20
PRIMER_INTERNAL_3=70,20
PRIMER_LEFT_3_TM=59.139
PRIMER_RIGHT_3_TM=59.218
PRIMER_INTERNAL_3_TM=57.668
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=3.861466
PRIMER_LEFT_4_PENALTY=3.544107
PRIMER_RIGHT_4_PENALTY=0.317359
PRIMER_INTERNAL_4_PENALTY=2.331778
PRIMER_LEFT_4_SEQUENCE=TGTCCTTGACATCAACATCAGAA
PRIMER_RIGHT_4_SEQUENCE=TGAAACCTCCGTCATGGATT
PRIMER_INTERNAL_4_SEQUENCE=ACCCTAACATCCTTGGCTTT
PRIMER_LEFT_4=36,23
PRIMER_RIGHT_4=112,20
PRIMER_INTERNAL_4=70,20
PRIMER_LEFT_4_TM=60.544
PRIMER_RIGHT_4_TM=60.317
PRIMER_INTERNAL_4_TM=57.668
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH739
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTAGAGGCTGTATGGCCTCCAGGAAAAGTAGCTTTAATGCACTGCAGATGGCACCAAAAGTCAGGGACACCAAAAACCAAAAGAAACAGAAAGGCAGACAGAGAGGCAAAGAGGGGAGCAATGATTGCATCACATTTTAAAGAGGAAGCCTTAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCT
SEQUENCE_INCLUDED_REGION=36,156
P3_COMMENT=CL is GTATGGCCTCCAGGAAAAGT
P3_COMMENT=CR is TTAAAATGTGATGCAATCATTG
P3_COMMENT=CM is GCTTTAATGCACTGCAGATG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.285274
PRIMER_LEFT_0_PENALTY=0.151533
PRIMER_RIGHT_0_PENALTY=0.133741
PRIMER_INTERNAL_0_PENALTY=0.111077
PRIMER_LEFT_0_SEQUENCE=GGCCTCCAGGAAAAGTAGCT
PRIMER_RIGHT_0_SEQUENCE=TTGCCTCTCTGTCTGCCTTT
PRIMER_INTERNAL_0_SEQUENCE=AGATGGCACCAAAAGTCAGG
PRIMER_LEFT_0=52,20
PRIMER_RIGHT_0=147,20
PRIMER_INTERNAL_0=84,20
PRIMER_LEFT_0_TM=59.848
PRIMER_RIGHT_0_TM=60.134
PRIMER_INTERNAL_0_TM=60.111
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=0.285274
PRIMER_LEFT_1_PENALTY=0.151533
PRIMER_RIGHT_1_PENALTY=0.133741
PRIMER_INTERNAL_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=GGCCTCCAGGAAAAGTAGCT
PRIMER_RIGHT_1_SEQUENCE=TTTGCCTCTCTGTCTGCCTT
PRIMER_INTERNAL_1_SEQUENCE=AGATGGCACCAAAAGTCAGG
PRIMER_LEFT_1=52,20
PRIMER_RIGHT_1=148,20
PRIMER_INTERNAL_1=84,20
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=60.134
PRIMER_INTERNAL_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=0.294569
PRIMER_LEFT_2_PENALTY=0.160828
PRIMER_RIGHT_2_PENALTY=0.133741
PRIMER_INTERNAL_2_PENALTY=0.399325
PRIMER_LEFT_2_SEQUENCE=TTTAATGCACTGCAGATGGC
PRIMER_RIGHT_2_SEQUENCE=TTGCCTCTCTGTCTGCCTTT
PRIMER_INTERNAL_2_SEQUENCE=ACCAAAAGTCAGGGACACCA
PRIMER_LEFT_2=71,20
PRIMER_RIGHT_2=147,20
PRIMER_INTERNAL_2=91,20
PRIMER_LEFT_2_TM=59.839
PRIMER_RIGHT_2_TM=60.134
PRIMER_INTERNAL_2_TM=60.399
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=0.294569
PRIMER_LEFT_3_PENALTY=0.160828
PRIMER_RIGHT_3_PENALTY=0.133741
PRIMER_INTERNAL_3_PENALTY=0.399325
PRIMER_LEFT_3_SEQUENCE=TTTAATGCACTGCAGATGGC
PRIMER_RIGHT_3_SEQUENCE=TTTGCCTCTCTGTCTGCCTT
PRIMER_INTERNAL_3_SEQUENCE=ACCAAAAGTCAGGGACACCA
PRIMER_LEFT_3=71,20
PRIMER_RIGHT_3=148,20
PRIMER_INTERNAL_3=91,20
PRIMER_LEFT_3_TM=59.839
PRIMER_RIGHT_3_TM=60.134
PRIMER_INTERNAL_3_TM=60.399
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=0.406531
PRIMER_LEFT_4_PENALTY=0.151533
PRIMER_RIGHT_4_PENALTY=0.254998
PRIMER_INTERNAL_4_PENALTY=0.111077
PRIMER_LEFT_4_SEQUENCE=GGCCTCCAGGAAAAGTAGCT
PRIMER_RIGHT_4_SEQUENCE=CTTTGCCTCTCTGTCTGCCT
PRIMER_INTERNAL_4_SEQUENCE=AGATGGCACCAAAAGTCAGG
PRIMER_LEFT_4=52,20
PRIMER_RIGHT_4=149,20
PRIMER_INTERNAL_4=84,20
PRIMER_LEFT_4_TM=59.848
PRIMER_RIGHT_4_TM=59.745
PRIMER_INTERNAL_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH745
SEQUENCE_TEMPLATE=GCATGCCCTGCTCAGACTGTGCACTACAGACCAATCAACTTGAGCAGTTCAGAGAACAAAATAGTCAATGTTAGCCTGGCAGATCTTAGAGGTNGAAATCGCCCCAATACAGGGCCCTTATACACAGAGGCCGATCGAGTCATACAGAGAACAAATTCAATGCAGCAGTTGGAACAGTGGATTAAAATCCAGAAGGGGAGGGGTCATGAAGANGAAACCAGGGGGTAAGTGTCTGTCTTGTCATTATGAACCCAGGTTTCTTTGATGCTGTGTTGGTGGAGATTGGTTTCTGAG
SEQUENCE_INCLUDED_REGION=0,294
P3_COMMENT=CL is AGTCAATGTTAGCCTGGCAG
P3_COMMENT=CR is AATCTCCACCAACACAGCAT
P3_COMMENT=CM is GAAATCGCCCCAATACAGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.338549
PRIMER_LEFT_0_PENALTY=0.088413
PRIMER_RIGHT_0_PENALTY=0.250137
PRIMER_INTERNAL_0_PENALTY=0.095488
PRIMER_LEFT_0_SEQUENCE=CCCTTATACACAGAGGCCGA
PRIMER_RIGHT_0_SEQUENCE=CCCTCCCCTTCTGGATTTTA
PRIMER_INTERNAL_0_SEQUENCE=ATGCAGCAGTTGGAACAGTG
PRIMER_LEFT_0=114,20
PRIMER_RIGHT_0=201,20
PRIMER_INTERNAL_0=159,20
PRIMER_LEFT_0_TM=60.088
PRIMER_RIGHT_0_TM=60.250
PRIMER_INTERNAL_0_TM=59.905
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=11.4000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_1_PENALTY=0.401678
PRIMER_LEFT_1_PENALTY=0.250137
PRIMER_RIGHT_1_PENALTY=0.151542
PRIMER_INTERNAL_1_PENALTY=0.575181
PRIMER_LEFT_1_SEQUENCE=TAAAATCCAGAAGGGGAGGG
PRIMER_RIGHT_1_SEQUENCE=CCACCAACACAGCATCAAAG
PRIMER_INTERNAL_1_SEQUENCE=CCAGGGGGTAAGTGTCTGTC
PRIMER_LEFT_1=182,20
PRIMER_RIGHT_1=278,20
PRIMER_INTERNAL_1=217,20
PRIMER_LEFT_1_TM=60.250
PRIMER_RIGHT_1_TM=60.152
PRIMER_INTERNAL_1_TM=59.425
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=0.490609
PRIMER_LEFT_2_PENALTY=0.240472
PRIMER_RIGHT_2_PENALTY=0.250137
PRIMER_INTERNAL_2_PENALTY=0.095488
PRIMER_LEFT_2_SEQUENCE=AGGCCGATCGAGTCATACAG
PRIMER_RIGHT_2_SEQUENCE=CCCTCCCCTTCTGGATTTTA
PRIMER_INTERNAL_2_SEQUENCE=ATGCAGCAGTTGGAACAGTG
PRIMER_LEFT_2=127,20
PRIMER_RIGHT_2=201,20
PRIMER_INTERNAL_2=159,20
PRIMER_LEFT_2_TM=60.240
PRIMER_RIGHT_2_TM=60.250
PRIMER_INTERNAL_2_TM=59.905
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=0.490609
PRIMER_LEFT_3_PENALTY=0.240472
PRIMER_RIGHT_3_PENALTY=0.250137
PRIMER_INTERNAL_3_PENALTY=0.095488
PRIMER_LEFT_3_SEQUENCE=ATACACAGAGGCCGATCGAG
PRIMER_RIGHT_3_SEQUENCE=CCCTCCCCTTCTGGATTTTA
PRIMER_INTERNAL_3_SEQUENCE=ATGCAGCAGTTGGAACAGTG
PRIMER_LEFT_3=119,20
PRIMER_RIGHT_3=201,20
PRIMER_INTERNAL_3=159,20
PRIMER_LEFT_3_TM=60.240
PRIMER_RIGHT_3_TM=60.250
PRIMER_INTERNAL_3_TM=59.905
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=0.670668
PRIMER_LEFT_4_PENALTY=0.095488
PRIMER_RIGHT_4_PENALTY=0.575181
PRIMER_INTERNAL_4_PENALTY=0.250137
PRIMER_LEFT_4_SEQUENCE=ATGCAGCAGTTGGAACAGTG
PRIMER_RIGHT_4_SEQUENCE=GACAGACACTTACCCCCTGG
PRIMER_INTERNAL_4_SEQUENCE=TAAAATCCAGAAGGGGAGGG
PRIMER_LEFT_4=159,20
PRIMER_RIGHT_4=236,20
PRIMER_INTERNAL_4=182,20
PRIMER_LEFT_4_TM=59.905
PRIMER_RIGHT_4_TM=59.425
PRIMER_INTERNAL_4_TM=60.250
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH750
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACAGGCAAGGGCCACACCCAGAAGAGGAGGGAGGTGGACTTCAAGTGGCCTCGTAAAACAAAGACACATTCTCCTATAATGTTCAGTCATTCGGGATGTGTTTTAGACTAAGGAAGATTTCCACTCCAGGTCCTGCCAGAGNTGCTGTGAGAAACTATCACGCTGGTCCTCTCATCTGGATCACCAGACAACAG
SEQUENCE_INCLUDED_REGION=33,196
P3_COMMENT=CL is CTTCAAGTGGCCTCGTAAAA
P3_COMMENT=CR is CTGGTGATCCAGATGAGAGG
P3_COMMENT=CM is CACATTCTCCTATAATGTTCAGTCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.749424
PRIMER_LEFT_0_PENALTY=0.254613
PRIMER_RIGHT_0_PENALTY=0.494811
PRIMER_INTERNAL_0_PENALTY=1.067229
PRIMER_LEFT_0_SEQUENCE=GGACTTCAAGTGGCCTCGTA
PRIMER_RIGHT_0_SEQUENCE=CAGGACCTGGAGTGGAAATC
PRIMER_INTERNAL_0_SEQUENCE=TCAGTCATTCGGGATGTGTT
PRIMER_LEFT_0=70,20
PRIMER_RIGHT_0=169,20
PRIMER_INTERNAL_0=117,20
PRIMER_LEFT_0_TM=60.255
PRIMER_RIGHT_0_TM=59.505
PRIMER_INTERNAL_0_TM=58.933
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=1.177496
PRIMER_LEFT_1_PENALTY=0.682685
PRIMER_RIGHT_1_PENALTY=0.494811
PRIMER_INTERNAL_1_PENALTY=1.067229
PRIMER_LEFT_1_SEQUENCE=TCAAGTGGCCTCGTAAAACA
PRIMER_RIGHT_1_SEQUENCE=CAGGACCTGGAGTGGAAATC
PRIMER_INTERNAL_1_SEQUENCE=TCAGTCATTCGGGATGTGTT
PRIMER_LEFT_1=75,20
PRIMER_RIGHT_1=169,20
PRIMER_INTERNAL_1=117,20
PRIMER_LEFT_1_TM=59.317
PRIMER_RIGHT_1_TM=59.505
PRIMER_INTERNAL_1_TM=58.933
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=1.298512
PRIMER_LEFT_2_PENALTY=1.067229
PRIMER_RIGHT_2_PENALTY=0.231283
PRIMER_INTERNAL_2_PENALTY=0.494811
PRIMER_LEFT_2_SEQUENCE=TCAGTCATTCGGGATGTGTT
PRIMER_RIGHT_2_SEQUENCE=GATGAGAGGACCAGCGTGAT
PRIMER_INTERNAL_2_SEQUENCE=GATTTCCACTCCAGGTCCTG
PRIMER_LEFT_2=117,20
PRIMER_RIGHT_2=210,20
PRIMER_INTERNAL_2=150,20
PRIMER_LEFT_2_TM=58.933
PRIMER_RIGHT_2_TM=60.231
PRIMER_INTERNAL_2_TM=59.505
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=7.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=94
PRIMER_PAIR_3_PENALTY=1.298512
PRIMER_LEFT_3_PENALTY=1.067229
PRIMER_RIGHT_3_PENALTY=0.231283
PRIMER_INTERNAL_3_PENALTY=0.494811
PRIMER_LEFT_3_SEQUENCE=TTCAGTCATTCGGGATGTGT
PRIMER_RIGHT_3_SEQUENCE=GATGAGAGGACCAGCGTGAT
PRIMER_INTERNAL_3_SEQUENCE=GATTTCCACTCCAGGTCCTG
PRIMER_LEFT_3=116,20
PRIMER_RIGHT_3=210,20
PRIMER_INTERNAL_3=150,20
PRIMER_LEFT_3_TM=58.933
PRIMER_RIGHT_3_TM=60.231
PRIMER_INTERNAL_3_TM=59.505
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=1.298512
PRIMER_LEFT_4_PENALTY=1.067229
PRIMER_RIGHT_4_PENALTY=0.231283
PRIMER_INTERNAL_4_PENALTY=0.494811
PRIMER_LEFT_4_SEQUENCE=GTTCAGTCATTCGGGATGTG
PRIMER_RIGHT_4_SEQUENCE=GATGAGAGGACCAGCGTGAT
PRIMER_INTERNAL_4_SEQUENCE=GATTTCCACTCCAGGTCCTG
PRIMER_LEFT_4=115,20
PRIMER_RIGHT_4=210,20
PRIMER_INTERNAL_4=150,20
PRIMER_LEFT_4_TM=58.933
PRIMER_RIGHT_4_TM=60.231
PRIMER_INTERNAL_4_TM=59.505
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH754
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATGATATGGAGAAAAACATTATCTTTCAAAGCTGAAACTTAAGCCTGTGTATTAAAATTNCTTTTCACAACTATTGATAAGACTACCTGGAAAGGAAAGTTGCCTCTATAAGAAATACTAACTGTATCATTGTAGTCAACAGTGAAAGTGAAAAAGAAAGANTTTTTGGAGAAAGCATTAAAAAGTGAAGAGAACAAAGTGTNGCAGACATTTCAACCCAAAATAACATAATTTCCAACGGNGGGTGAA
SEQUENCE_INCLUDED_REGION=35,253
P3_COMMENT=CL is TCTTTCAAAGCTGAAACTTAAGC
P3_COMMENT=CR is TTTTGGGTTGAAATGTCTGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.139276
PRIMER_LEFT_0_PENALTY=1.091240
PRIMER_RIGHT_0_PENALTY=5.048037
PRIMER_INTERNAL_0_PENALTY=5.848686
PRIMER_LEFT_0_SEQUENCE=TGGAAAGGAAAGTTGCCTCT
PRIMER_RIGHT_0_SEQUENCE=CACTTTTTAATGCTTTCTCCAAAAA
PRIMER_INTERNAL_0_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
PRIMER_LEFT_0=126,20
PRIMER_RIGHT_0=225,25
PRIMER_INTERNAL_0=174,24
PRIMER_LEFT_0_TM=58.909
PRIMER_RIGHT_0_TM=60.048
PRIMER_INTERNAL_0_TM=58.151
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=28.000
PRIMER_INTERNAL_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=6.154243
PRIMER_LEFT_1_PENALTY=1.091240
PRIMER_RIGHT_1_PENALTY=5.063004
PRIMER_INTERNAL_1_PENALTY=5.848686
PRIMER_LEFT_1_SEQUENCE=TGGAAAGGAAAGTTGCCTCT
PRIMER_RIGHT_1_SEQUENCE=CACTTTTTAATGCTTTCTCCAAAA
PRIMER_INTERNAL_1_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
PRIMER_LEFT_1=126,20
PRIMER_RIGHT_1=225,24
PRIMER_INTERNAL_1=174,24
PRIMER_LEFT_1_TM=58.909
PRIMER_RIGHT_1_TM=58.937
PRIMER_INTERNAL_1_TM=58.151
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=29.167
PRIMER_INTERNAL_1_GC_PERCENT=29.167
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=6.371422
PRIMER_LEFT_2_PENALTY=1.091240
PRIMER_RIGHT_2_PENALTY=5.280182
PRIMER_INTERNAL_2_PENALTY=5.848686
PRIMER_LEFT_2_SEQUENCE=TGGAAAGGAAAGTTGCCTCT
PRIMER_RIGHT_2_SEQUENCE=CACTTTTTAATGCTTTCTCCAAA
PRIMER_INTERNAL_2_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
PRIMER_LEFT_2=126,20
PRIMER_RIGHT_2=225,23
PRIMER_INTERNAL_2=174,24
PRIMER_LEFT_2_TM=58.909
PRIMER_RIGHT_2_TM=57.720
PRIMER_INTERNAL_2_TM=58.151
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_INTERNAL_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=6.599702
PRIMER_LEFT_3_PENALTY=0.600817
PRIMER_RIGHT_3_PENALTY=5.998885
PRIMER_INTERNAL_3_PENALTY=5.848686
PRIMER_LEFT_3_SEQUENCE=CCTGGAAAGGAAAGTTGCCT
PRIMER_RIGHT_3_SEQUENCE=CTTTTTAATGCTTTCTCCAAAAA
PRIMER_INTERNAL_3_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA
PRIMER_LEFT_3=124,20
PRIMER_RIGHT_3=223,23
PRIMER_INTERNAL_3=174,24
PRIMER_LEFT_3_TM=60.601
PRIMER_RIGHT_3_TM=57.001
PRIMER_INTERNAL_3_TM=58.151
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=26.087
PRIMER_INTERNAL_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=7.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=6.634142
PRIMER_LEFT_4_PENALTY=6.158436
PRIMER_RIGHT_4_PENALTY=0.475706
PRIMER_INTERNAL_4_PENALTY=8.892047
PRIMER_LEFT_4_SEQUENCE=GGAGAAAAACATTATCTTTCAAAGC
PRIMER_RIGHT_4_SEQUENCE=GGCAACTTTCCTTTCCAGGT
PRIMER_INTERNAL_4_SEQUENCE=TGAAACTTAAGCCTGTGTATTAAAATT
PRIMER_LEFT_4=46,25
PRIMER_RIGHT_4=142,20
PRIMER_INTERNAL_4=71,27
PRIMER_LEFT_4_TM=58.842
PRIMER_RIGHT_4_TM=60.476
PRIMER_INTERNAL_4_TM=58.108
PRIMER_LEFT_4_GC_PERCENT=32.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=25.926
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=97
=
SEQUENCE_ID=MH757
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGATAGGTNTTTAAATTAGAAAGTTACCTGTATTAGCCATATTTACATTTCTGTAGGCTGTACACTCTAAGAATTCTAGTTTAAAAGTCACTGGTTCATTTCAGTATGCATAGCCAGTCTCAAGTTGTCACATTTTGTTCATCAGTTTTCCTTATCTCTTGCATTTTTCAAAATTCTGTGACTTTCAAGCACATTGAACCTTCTTACTTTTACANCTTCACTATCATTAATTATTTTACTTATTATGTTAACACTGCATTATCTGC
SEQUENCE_INCLUDED_REGION=35,113
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH760
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATGGGATATTATCATTCAGAGTNNTCCTGTGCCAACGNAGTCTGGGCGCTGGGAACATCTGGAGTCAACATTCTGGCCCATCACAACTCCTTAGGGACCTCCATTCCTCAGGGAATGCACTCAGATCTCAGGCACCAAAACCAACATTGTCTGCTCTTCCTTCCTTTTCTGTCTGGACTTTGTTGGCTGTTGAAAAAAGGAATGAGATTTTCCAAAAGTTTCTTCACAAACTGCTAATGCCAGTACAGGCCAGTTCCATGATTTTACCAGTTCAATCCAGTGATATAG
SEQUENCE_INCLUDED_REGION=36,267
P3_COMMENT=CL is CATCTGGAGTCAACATTCTGG
P3_COMMENT=CR is AATCATGGAACTGGCCTGTA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.086626
PRIMER_LEFT_0_PENALTY=0.066262
PRIMER_RIGHT_0_PENALTY=0.020364
PRIMER_INTERNAL_0_PENALTY=0.245123
PRIMER_LEFT_0_SEQUENCE=AGTCAACATTCTGGCCCATC
PRIMER_RIGHT_0_SEQUENCE=TGGTGCCTGAGATCTGAGTG
PRIMER_INTERNAL_0_SEQUENCE=ACCTCCATTCCTCAGGGAAT
PRIMER_LEFT_0=101,20
PRIMER_RIGHT_0=175,20
PRIMER_INTERNAL_0=135,20
PRIMER_LEFT_0_TM=59.934
PRIMER_RIGHT_0_TM=59.980
PRIMER_INTERNAL_0_TM=59.755
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.086626
PRIMER_LEFT_1_PENALTY=0.066262
PRIMER_RIGHT_1_PENALTY=0.020364
PRIMER_INTERNAL_1_PENALTY=0.245123
PRIMER_LEFT_1_SEQUENCE=GAGTCAACATTCTGGCCCAT
PRIMER_RIGHT_1_SEQUENCE=TGGTGCCTGAGATCTGAGTG
PRIMER_INTERNAL_1_SEQUENCE=ACCTCCATTCCTCAGGGAAT
PRIMER_LEFT_1=100,20
PRIMER_RIGHT_1=175,20
PRIMER_INTERNAL_1=135,20
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=59.980
PRIMER_INTERNAL_1_TM=59.755
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=0.137642
PRIMER_LEFT_2_PENALTY=0.127097
PRIMER_RIGHT_2_PENALTY=0.010545
PRIMER_INTERNAL_2_PENALTY=3.188449
PRIMER_LEFT_2_SEQUENCE=TGTCTGGACTTTGTTGGCTG
PRIMER_RIGHT_2_SEQUENCE=ATGGAACTGGCCTGTACTGG
PRIMER_INTERNAL_2_SEQUENCE=CCAAAAGTTTCTTCACAAACTGC
PRIMER_LEFT_2=208,20
PRIMER_RIGHT_2=297,20
PRIMER_INTERNAL_2=250,23
PRIMER_LEFT_2_TM=59.873
PRIMER_RIGHT_2_TM=59.989
PRIMER_INTERNAL_2_TM=60.188
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=0.200048
PRIMER_LEFT_3_PENALTY=0.072951
PRIMER_RIGHT_3_PENALTY=0.127097
PRIMER_INTERNAL_3_PENALTY=0.495173
PRIMER_LEFT_3_SEQUENCE=CATTCCTCAGGGAATGCACT
PRIMER_RIGHT_3_SEQUENCE=CAGCCAACAAAGTCCAGACA
PRIMER_INTERNAL_3_SEQUENCE=ATCTCAGGCACCAAAACCAA
PRIMER_LEFT_3=140,20
PRIMER_RIGHT_3=227,20
PRIMER_INTERNAL_3=163,20
PRIMER_LEFT_3_TM=60.073
PRIMER_RIGHT_3_TM=59.873
PRIMER_INTERNAL_3_TM=60.495
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=9.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=0.268608
PRIMER_LEFT_4_PENALTY=0.127097
PRIMER_RIGHT_4_PENALTY=0.141511
PRIMER_INTERNAL_4_PENALTY=3.188449
PRIMER_LEFT_4_SEQUENCE=TGTCTGGACTTTGTTGGCTG
PRIMER_RIGHT_4_SEQUENCE=GAACTGGCCTGTACTGGCAT
PRIMER_INTERNAL_4_SEQUENCE=CCAAAAGTTTCTTCACAAACTGC
PRIMER_LEFT_4=208,20
PRIMER_RIGHT_4=294,20
PRIMER_INTERNAL_4=250,23
PRIMER_LEFT_4_TM=59.873
PRIMER_RIGHT_4_TM=60.142
PRIMER_INTERNAL_4_TM=60.188
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH761
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTAGAGANTCTCACAAGCAAAGTNTAAAATGTTTCCTATCTGGATGCTTGTGATAAATGCAGATTCCTGGGTCTCATCCCAGACTTTCAGAATCTGAATGCCTGAGATATGGCCCAGGAGAGTGCATTAAAACAAAAACAATAACANTACTCCTCCAGGTGCATCTTAAGCCTATCAGAGATTTAATGTGAGACATAGAATGTGAGAATCTTTGTTCTTGATTCCTGAGGTTATATTTTATTCAAACATAAACCCCGNTACACAACGTCCAGCATGCAGTGGG
SEQUENCE_INCLUDED_REGION=36,288
P3_COMMENT=CL is TAAAATGTTTCCTATCTGGATGC
P3_COMMENT=CR is ATATAACCTCAGGAATCAAGAACA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.835978
PRIMER_LEFT_0_PENALTY=0.622472
PRIMER_RIGHT_0_PENALTY=0.213506
PRIMER_INTERNAL_0_PENALTY=2.044832
PRIMER_LEFT_0_SEQUENCE=ATGCAGATTCCTGGGTCTCA
PRIMER_RIGHT_0_SEQUENCE=TTTAATGCACTCTCCTGGGC
PRIMER_INTERNAL_0_SEQUENCE=TTCAGAATCTGAATGCCTGAGA
PRIMER_LEFT_0=97,20
PRIMER_RIGHT_0=171,20
PRIMER_INTERNAL_0=126,22
PRIMER_LEFT_0_TM=60.622
PRIMER_RIGHT_0_TM=60.214
PRIMER_INTERNAL_0_TM=59.955
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=11.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=1.119764
PRIMER_LEFT_1_PENALTY=0.906258
PRIMER_RIGHT_1_PENALTY=0.213506
PRIMER_INTERNAL_1_PENALTY=2.044832
PRIMER_LEFT_1_SEQUENCE=AATGCAGATTCCTGGGTCTC
PRIMER_RIGHT_1_SEQUENCE=TTTAATGCACTCTCCTGGGC
PRIMER_INTERNAL_1_SEQUENCE=TTCAGAATCTGAATGCCTGAGA
PRIMER_LEFT_1=96,20
PRIMER_RIGHT_1=171,20
PRIMER_INTERNAL_1=126,22
PRIMER_LEFT_1_TM=59.094
PRIMER_RIGHT_1_TM=60.214
PRIMER_INTERNAL_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=11.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=1.136983
PRIMER_LEFT_2_PENALTY=0.973324
PRIMER_RIGHT_2_PENALTY=0.163658
PRIMER_INTERNAL_2_PENALTY=4.285317
PRIMER_LEFT_2_SEQUENCE=TGAATGCCTGAGATATGGCC
PRIMER_RIGHT_2_SEQUENCE=GCTTAAGATGCACCTGGAGG
PRIMER_INTERNAL_2_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA
PRIMER_LEFT_2=135,20
PRIMER_RIGHT_2=211,20
PRIMER_INTERNAL_2=155,24
PRIMER_LEFT_2_TM=60.973
PRIMER_RIGHT_2_TM=59.836
PRIMER_INTERNAL_2_TM=59.715
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=77
PRIMER_PAIR_3_PENALTY=1.136983
PRIMER_LEFT_3_PENALTY=0.973324
PRIMER_RIGHT_3_PENALTY=0.163658
PRIMER_INTERNAL_3_PENALTY=4.285317
PRIMER_LEFT_3_SEQUENCE=TGAATGCCTGAGATATGGCC
PRIMER_RIGHT_3_SEQUENCE=GGCTTAAGATGCACCTGGAG
PRIMER_INTERNAL_3_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA
PRIMER_LEFT_3=135,20
PRIMER_RIGHT_3=212,20
PRIMER_INTERNAL_3=155,24
PRIMER_LEFT_3_TM=60.973
PRIMER_RIGHT_3_TM=59.836
PRIMER_INTERNAL_3_TM=59.715
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=78
PRIMER_PAIR_4_PENALTY=1.136983
PRIMER_LEFT_4_PENALTY=0.973324
PRIMER_RIGHT_4_PENALTY=0.163658
PRIMER_INTERNAL_4_PENALTY=4.285317
PRIMER_LEFT_4_SEQUENCE=TGAATGCCTGAGATATGGCC
PRIMER_RIGHT_4_SEQUENCE=AGGCTTAAGATGCACCTGGA
PRIMER_INTERNAL_4_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA
PRIMER_LEFT_4=135,20
PRIMER_RIGHT_4=213,20
PRIMER_INTERNAL_4=155,24
PRIMER_LEFT_4_TM=60.973
PRIMER_RIGHT_4_TM=59.836
PRIMER_INTERNAL_4_TM=59.715
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH764
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAAAAAAGAGTNNAGTTTCAATACAAAAGGTTAAAAGAAAGAAAGGTCAGAGAAGCAAGTACGATACAGCCAAAGGGCCAGGCAAAATGAAGGTAAAACATAGAGACAAAATCGAGAATCAAAGTACGTTTGAGCATAGGAATGTCCTGATGAAAGTGGCACTTACTGAATATGAATATTGAGGCTAGTTTCAAGGGGACACACATAAGGCCAAGAAGTCAGATGGGAGGATGTCATAATAATCTACATATAATATGGTA
SEQUENCE_INCLUDED_REGION=35,261
P3_COMMENT=CL is AAAGAAAGAAAGGTCAGAGAAGC
P3_COMMENT=CR is TGACATCCTCCCATCTGACT
P3_COMMENT=CM is AGTACGATACAGCCAAAGGGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.392166
PRIMER_LEFT_0_PENALTY=0.074231
PRIMER_RIGHT_0_PENALTY=0.317935
PRIMER_INTERNAL_0_PENALTY=3.301915
PRIMER_LEFT_0_SEQUENCE=GCCAGGCAAAATGAAGGTAA
PRIMER_RIGHT_0_SEQUENCE=GTGCCACTTTCATCAGGACA
PRIMER_INTERNAL_0_SEQUENCE=CGAGAATCAAAGTACGTTTGAGC
PRIMER_LEFT_0=112,20
PRIMER_RIGHT_0=197,20
PRIMER_INTERNAL_0=148,23
PRIMER_LEFT_0_TM=60.074
PRIMER_RIGHT_0_TM=59.682
PRIMER_INTERNAL_0_TM=60.302
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=86
PRIMER_PAIR_1_PENALTY=0.514838
PRIMER_LEFT_1_PENALTY=0.317935
PRIMER_RIGHT_1_PENALTY=0.196903
PRIMER_INTERNAL_1_PENALTY=0.192918
PRIMER_LEFT_1_SEQUENCE=TGTCCTGATGAAAGTGGCAC
PRIMER_RIGHT_1_SEQUENCE=CTCCCATCTGACTTCTTGGC
PRIMER_INTERNAL_1_SEQUENCE=TGAGGCTAGTTTCAAGGGGA
PRIMER_LEFT_1=178,20
PRIMER_RIGHT_1=264,20
PRIMER_INTERNAL_1=215,20
PRIMER_LEFT_1_TM=59.682
PRIMER_RIGHT_1_TM=59.803
PRIMER_INTERNAL_1_TM=59.807
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=0.669082
PRIMER_LEFT_2_PENALTY=0.317935
PRIMER_RIGHT_2_PENALTY=0.351148
PRIMER_INTERNAL_2_PENALTY=0.192918
PRIMER_LEFT_2_SEQUENCE=TGTCCTGATGAAAGTGGCAC
PRIMER_RIGHT_2_SEQUENCE=CCTCCCATCTGACTTCTTGG
PRIMER_INTERNAL_2_SEQUENCE=TGAGGCTAGTTTCAAGGGGA
PRIMER_LEFT_2=178,20
PRIMER_RIGHT_2=265,20
PRIMER_INTERNAL_2=215,20
PRIMER_LEFT_2_TM=59.682
PRIMER_RIGHT_2_TM=59.649
PRIMER_INTERNAL_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=0.984533
PRIMER_LEFT_3_PENALTY=0.074231
PRIMER_RIGHT_3_PENALTY=0.910302
PRIMER_INTERNAL_3_PENALTY=3.301915
PRIMER_LEFT_3_SEQUENCE=GCCAGGCAAAATGAAGGTAA
PRIMER_RIGHT_3_SEQUENCE=TGCCACTTTCATCAGGACAT
PRIMER_INTERNAL_3_SEQUENCE=CGAGAATCAAAGTACGTTTGAGC
PRIMER_LEFT_3=112,20
PRIMER_RIGHT_3=196,20
PRIMER_INTERNAL_3=148,23
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.090
PRIMER_INTERNAL_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=1.034061
PRIMER_LEFT_4_PENALTY=0.317935
PRIMER_RIGHT_4_PENALTY=0.716126
PRIMER_INTERNAL_4_PENALTY=0.192918
PRIMER_LEFT_4_SEQUENCE=TGTCCTGATGAAAGTGGCAC
PRIMER_RIGHT_4_SEQUENCE=CCATCTGACTTCTTGGCCTT
PRIMER_INTERNAL_4_SEQUENCE=TGAGGCTAGTTTCAAGGGGA
PRIMER_LEFT_4=178,20
PRIMER_RIGHT_4=261,20
PRIMER_INTERNAL_4=215,20
PRIMER_LEFT_4_TM=59.682
PRIMER_RIGHT_4_TM=59.284
PRIMER_INTERNAL_4_TM=59.807
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=84
=
SEQUENCE_ID=MH765
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATTTGATCAAGGAGAGAGTCTATGTCCTTATACAGGTTAATTAGACAATCTGATAACAGTCCTATTTGTGACTAGTTACTCTGAGTNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
SEQUENCE_INCLUDED_REGION=36,94
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH766
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGTGTNTTTCTCCCTGCCTGGAGCCTGGAGCCTGGGGTCTGGGCCAGGAATTCCCAGGCTAGGGTTGGACTCCAGGTGCAGGAAGCAGCCTGAGGCCCTGTGCAGGTCCAGATCTTCTCAGCCAGCAGGGTATGCCCCCACACAGAGGAGAGAGTNGACTGTGGGTGCCAGGCTGGGGGTGCCTGCAGAGCCTCCAAATCAGTGAACCTCTTCTCTGAGGGTCTCTGTAGGGGGGTGTGAACTTAAACTTGAACTTAAATGAGAGCATCTTAAGCCAGAGGACCAAGTTTTAG
SEQUENCE_INCLUDED_REGION=35,164
P3_COMMENT=CL is TTTCTCCCTGCCTGGAGC
P3_COMMENT=CR is CTCTCCTCTGTGTGGGGG
P3_COMMENT=CM is GGGTCTGGGCCAGGAATT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.978351
PRIMER_LEFT_0_PENALTY=0.431941
PRIMER_RIGHT_0_PENALTY=0.546410
PRIMER_INTERNAL_0_PENALTY=2.388026
PRIMER_LEFT_0_SEQUENCE=CTAGGGTTGGACTCCAGGTG
PRIMER_RIGHT_0_SEQUENCE=ATACCCTGCTGGCTGAGAAG
PRIMER_INTERNAL_0_SEQUENCE=CCCTGTGCAGGTCCAGAT
PRIMER_LEFT_0=95,20
PRIMER_RIGHT_0=169,20
PRIMER_INTERNAL_0=132,18
PRIMER_LEFT_0_TM=59.568
PRIMER_RIGHT_0_TM=59.454
PRIMER_INTERNAL_0_TM=59.612
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=1.057041
PRIMER_LEFT_1_PENALTY=0.510631
PRIMER_RIGHT_1_PENALTY=0.546410
PRIMER_INTERNAL_1_PENALTY=2.388026
PRIMER_LEFT_1_SEQUENCE=GCTAGGGTTGGACTCCAGGT
PRIMER_RIGHT_1_SEQUENCE=ATACCCTGCTGGCTGAGAAG
PRIMER_INTERNAL_1_SEQUENCE=CCCTGTGCAGGTCCAGAT
PRIMER_LEFT_1=94,20
PRIMER_RIGHT_1=169,20
PRIMER_INTERNAL_1=132,18
PRIMER_LEFT_1_TM=60.511
PRIMER_RIGHT_1_TM=59.454
PRIMER_INTERNAL_1_TM=59.612
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=1.113238
PRIMER_LEFT_2_PENALTY=0.816516
PRIMER_RIGHT_2_PENALTY=0.296722
PRIMER_INTERNAL_2_PENALTY=1.972643
PRIMER_LEFT_2_SEQUENCE=GAATTCCCAGGCTAGGGTTG
PRIMER_RIGHT_2_SEQUENCE=CCTGCTGGCTGAGAAGATCT
PRIMER_INTERNAL_2_SEQUENCE=GACTCCAGGTGCAGGAAGC
PRIMER_LEFT_2=84,20
PRIMER_RIGHT_2=165,20
PRIMER_INTERNAL_2=104,19
PRIMER_LEFT_2_TM=60.817
PRIMER_RIGHT_2_TM=59.703
PRIMER_INTERNAL_2_TM=60.973
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=63.158
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=1.162398
PRIMER_LEFT_3_PENALTY=0.431941
PRIMER_RIGHT_3_PENALTY=0.730458
PRIMER_INTERNAL_3_PENALTY=0.260757
PRIMER_LEFT_3_SEQUENCE=CTAGGGTTGGACTCCAGGTG
PRIMER_RIGHT_3_SEQUENCE=ACTCTCTCCTCTGTGTGGGG
PRIMER_INTERNAL_3_SEQUENCE=CCTGTGCAGGTCCAGATCTT
PRIMER_LEFT_3=95,20
PRIMER_RIGHT_3=191,20
PRIMER_INTERNAL_3=133,20
PRIMER_LEFT_3_TM=59.568
PRIMER_RIGHT_3_TM=59.270
PRIMER_INTERNAL_3_TM=60.261
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=97
PRIMER_PAIR_4_PENALTY=1.165557
PRIMER_LEFT_4_PENALTY=0.816516
PRIMER_RIGHT_4_PENALTY=0.349041
PRIMER_INTERNAL_4_PENALTY=1.972643
PRIMER_LEFT_4_SEQUENCE=GAATTCCCAGGCTAGGGTTG
PRIMER_RIGHT_4_SEQUENCE=TGGCTGAGAAGATCTGGACC
PRIMER_INTERNAL_4_SEQUENCE=GACTCCAGGTGCAGGAAGC
PRIMER_LEFT_4=84,20
PRIMER_RIGHT_4=160,20
PRIMER_INTERNAL_4=104,19
PRIMER_LEFT_4_TM=60.817
PRIMER_RIGHT_4_TM=60.349
PRIMER_INTERNAL_4_TM=60.973
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=63.158
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH767
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGTGCTGCAGTATCAATCCTCTGAGTNNACTAATATTCCGAGGATTAATACTCAGCCCTCTTGGGNTCNTAGATTTCTGGACCTACTTTGAAATGAGTCTTATACCCATCAAAATATAATGAAGAGGGCATGTCTGTGAAAATATGAAAACCATTCTTATTTTCCATATTAATGAGAAAAGTTAATTTATTTCTCACACTGGGACATTTTAAGTGAAAGGCATACCTTCAATGATACCATATAAATCTTGGATAG
SEQUENCE_INCLUDED_REGION=36,258
P3_COMMENT=CL is GGTGCTGCAGTATCAATCCT
P3_COMMENT=CR is TGGTATCATTGAAGGTATGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.062077
PRIMER_LEFT_0_PENALTY=2.659966
PRIMER_RIGHT_0_PENALTY=0.402111
PRIMER_INTERNAL_0_PENALTY=5.050278
PRIMER_LEFT_0_SEQUENCE=TTAATACTCAGCCCTCTTGGG
PRIMER_RIGHT_0_SEQUENCE=TCACAGACATGCCCTCTTCA
PRIMER_INTERNAL_0_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
PRIMER_LEFT_0=83,21
PRIMER_RIGHT_0=177,20
PRIMER_INTERNAL_0=113,25
PRIMER_LEFT_0_TM=58.340
PRIMER_RIGHT_0_TM=60.402
PRIMER_INTERNAL_0_TM=60.050
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=3.618241
PRIMER_LEFT_1_PENALTY=1.274264
PRIMER_RIGHT_1_PENALTY=2.343977
PRIMER_INTERNAL_1_PENALTY=6.803413
PRIMER_LEFT_1_SEQUENCE=AAGAGGGCATGTCTGTGAAAA
PRIMER_RIGHT_1_SEQUENCE=GCCTTTCACTTAAAATGTCCCA
PRIMER_INTERNAL_1_SEQUENCE=ATGAAAACCATTCTTATTTTCCAT
PRIMER_LEFT_1=160,21
PRIMER_RIGHT_1=259,22
PRIMER_INTERNAL_1=182,24
PRIMER_LEFT_1_TM=59.726
PRIMER_RIGHT_1_TM=60.344
PRIMER_INTERNAL_1_TM=57.197
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_INTERNAL_1_GC_PERCENT=25.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=3.729406
PRIMER_LEFT_2_PENALTY=3.327294
PRIMER_RIGHT_2_PENALTY=0.402111
PRIMER_INTERNAL_2_PENALTY=5.050278
PRIMER_LEFT_2_SEQUENCE=GATTAATACTCAGCCCTCTTGGG
PRIMER_RIGHT_2_SEQUENCE=TCACAGACATGCCCTCTTCA
PRIMER_INTERNAL_2_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
PRIMER_LEFT_2=81,23
PRIMER_RIGHT_2=177,20
PRIMER_INTERNAL_2=113,25
PRIMER_LEFT_2_TM=60.327
PRIMER_RIGHT_2_TM=60.402
PRIMER_INTERNAL_2_TM=60.050
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=3.729406
PRIMER_LEFT_3_PENALTY=3.327294
PRIMER_RIGHT_3_PENALTY=0.402111
PRIMER_INTERNAL_3_PENALTY=5.050278
PRIMER_LEFT_3_SEQUENCE=GGATTAATACTCAGCCCTCTTGG
PRIMER_RIGHT_3_SEQUENCE=TCACAGACATGCCCTCTTCA
PRIMER_INTERNAL_3_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
PRIMER_LEFT_3=80,23
PRIMER_RIGHT_3=177,20
PRIMER_INTERNAL_3=113,25
PRIMER_LEFT_3_TM=60.327
PRIMER_RIGHT_3_TM=60.402
PRIMER_INTERNAL_3_TM=60.050
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=3.731007
PRIMER_LEFT_4_PENALTY=3.328895
PRIMER_RIGHT_4_PENALTY=0.402111
PRIMER_INTERNAL_4_PENALTY=5.050278
PRIMER_LEFT_4_SEQUENCE=ATTAATACTCAGCCCTCTTGGG
PRIMER_RIGHT_4_SEQUENCE=TCACAGACATGCCCTCTTCA
PRIMER_INTERNAL_4_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=177,20
PRIMER_INTERNAL_4=113,25
PRIMER_LEFT_4_TM=58.671
PRIMER_RIGHT_4_TM=60.402
PRIMER_INTERNAL_4_TM=60.050
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH771
SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAGTNGGGAGAAAAAAATTACCTCAAACCCTACCAAGTGTCAAAGCGGCCTTAAAATATTGGTATTTAAAGGAGGTATAATACCAATAAGTCTGAAGTGATTAAGAGGTCTGAGTNAGGACATACAGGGCTGTGTTATGTAGTTTGTGACAGAAATCTTAAATTAAGACGGCAACATAGATGGTGTAACTTACTCGCAGACCTGAAAAAAAAGTGCTGTCAACCCCTCAACTCATGGTGATGGCACTTAGTGAGCATTGGTCTAATAAAGGGGCAAAGAGTGTATGAGTTTCTCCAATTCTGATTTTTTTTTTCTGG
SEQUENCE_INCLUDED_REGION=36,192
P3_COMMENT=CL is AGCGGCCTTAAAATATTGGT
P3_COMMENT=CR is GTTACACCATCTATGTTGCCG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.148265
PRIMER_LEFT_0_PENALTY=0.243575
PRIMER_RIGHT_0_PENALTY=2.904690
PRIMER_INTERNAL_0_PENALTY=6.929653
PRIMER_LEFT_0_SEQUENCE=GTGTCAAAGCGGCCTTAAAA
PRIMER_RIGHT_0_SEQUENCE=AACACAGCCCTGTATGTCCT
PRIMER_INTERNAL_0_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
PRIMER_LEFT_0=74,20
PRIMER_RIGHT_0=173,20
PRIMER_INTERNAL_0=125,26
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=57.095
PRIMER_INTERNAL_0_TM=59.070
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=38.462
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=3.184286
PRIMER_LEFT_1_PENALTY=0.279595
PRIMER_RIGHT_1_PENALTY=2.904690
PRIMER_INTERNAL_1_PENALTY=6.929653
PRIMER_LEFT_1_SEQUENCE=TGTCAAAGCGGCCTTAAAAT
PRIMER_RIGHT_1_SEQUENCE=AACACAGCCCTGTATGTCCT
PRIMER_INTERNAL_1_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
PRIMER_LEFT_1=75,20
PRIMER_RIGHT_1=173,20
PRIMER_INTERNAL_1=125,26
PRIMER_LEFT_1_TM=59.720
PRIMER_RIGHT_1_TM=57.095
PRIMER_INTERNAL_1_TM=59.070
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=38.462
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=4.059561
PRIMER_LEFT_2_PENALTY=0.279595
PRIMER_RIGHT_2_PENALTY=3.779966
PRIMER_INTERNAL_2_PENALTY=6.929653
PRIMER_LEFT_2_SEQUENCE=TGTCAAAGCGGCCTTAAAAT
PRIMER_RIGHT_2_SEQUENCE=TAACACAGCCCTGTATGTCCT
PRIMER_INTERNAL_2_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
PRIMER_LEFT_2=75,20
PRIMER_RIGHT_2=174,21
PRIMER_INTERNAL_2=125,26
PRIMER_LEFT_2_TM=59.720
PRIMER_RIGHT_2_TM=57.220
PRIMER_INTERNAL_2_TM=59.070
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=38.462
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=4.108099
PRIMER_LEFT_3_PENALTY=1.490704
PRIMER_RIGHT_3_PENALTY=2.617395
PRIMER_INTERNAL_3_PENALTY=6.929653
PRIMER_LEFT_3_SEQUENCE=AAAGCGGCCTTAAAATATTGG
PRIMER_RIGHT_3_SEQUENCE=CATAACACAGCCCTGTATGTCC
PRIMER_INTERNAL_3_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
PRIMER_LEFT_3=79,21
PRIMER_RIGHT_3=176,22
PRIMER_INTERNAL_3=125,26
PRIMER_LEFT_3_TM=59.509
PRIMER_RIGHT_3_TM=59.383
PRIMER_INTERNAL_3_TM=59.070
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=38.462
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=4.164547
PRIMER_LEFT_4_PENALTY=1.259857
PRIMER_RIGHT_4_PENALTY=2.904690
PRIMER_INTERNAL_4_PENALTY=6.929653
PRIMER_LEFT_4_SEQUENCE=TGTCAAAGCGGCCTTAAAATA
PRIMER_RIGHT_4_SEQUENCE=AACACAGCCCTGTATGTCCT
PRIMER_INTERNAL_4_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA
PRIMER_LEFT_4=75,21
PRIMER_RIGHT_4=173,20
PRIMER_INTERNAL_4=125,26
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=57.095
PRIMER_INTERNAL_4_TM=59.070
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=38.462
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH777
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTTCCAGGGAGATGAAGTTATTGAACATCCACTTTAGAAATAGAGCACCATGGCAAGTAGTGCTCTCTGAGGTTCAGGATCCTCTGCAGAGTNNATAATACATCTAGGAAGACATCAGGCTTTAAAAACTCTATGGAGCAACATGTCAACAAATTTGATTTAATATAGGAGAAAAAAAAATCCCCGCATATCTTAGTGCTGAGTGGACACTCTAG
SEQUENCE_INCLUDED_REGION=33,217
P3_COMMENT=CL is CCTTCCAGGGAGATGAAGTT
P3_COMMENT=CR is ACTCAGCACTAAGATATGCGG
P3_COMMENT=CM is TGAACATCCACTTTAGAAATAGAGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.408357
PRIMER_LEFT_0_PENALTY=1.059316
PRIMER_RIGHT_0_PENALTY=0.349041
PRIMER_INTERNAL_0_PENALTY=0.681621
PRIMER_LEFT_0_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
PRIMER_RIGHT_0_SEQUENCE=GCAGAGGATCCTGAACCTCA
PRIMER_INTERNAL_0_SEQUENCE=CACCATGGCAAGTAGTGCTC
PRIMER_LEFT_0=33,21
PRIMER_RIGHT_0=121,20
PRIMER_INTERNAL_0=80,20
PRIMER_LEFT_0_TM=60.059
PRIMER_RIGHT_0_TM=60.349
PRIMER_INTERNAL_0_TM=59.318
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=5.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=1.408357
PRIMER_LEFT_1_PENALTY=1.059316
PRIMER_RIGHT_1_PENALTY=0.349041
PRIMER_INTERNAL_1_PENALTY=0.681621
PRIMER_LEFT_1_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
PRIMER_RIGHT_1_SEQUENCE=TGCAGAGGATCCTGAACCTC
PRIMER_INTERNAL_1_SEQUENCE=CACCATGGCAAGTAGTGCTC
PRIMER_LEFT_1=33,21
PRIMER_RIGHT_1=122,20
PRIMER_INTERNAL_1=80,20
PRIMER_LEFT_1_TM=60.059
PRIMER_RIGHT_1_TM=60.349
PRIMER_INTERNAL_1_TM=59.318
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=8.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=1.408357
PRIMER_LEFT_2_PENALTY=1.059316
PRIMER_RIGHT_2_PENALTY=0.349041
PRIMER_INTERNAL_2_PENALTY=0.681621
PRIMER_LEFT_2_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
PRIMER_RIGHT_2_SEQUENCE=TCTGCAGAGGATCCTGAACC
PRIMER_INTERNAL_2_SEQUENCE=CACCATGGCAAGTAGTGCTC
PRIMER_LEFT_2=33,21
PRIMER_RIGHT_2=124,20
PRIMER_INTERNAL_2=80,20
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=60.349
PRIMER_INTERNAL_2_TM=59.318
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=1.654525
PRIMER_LEFT_3_PENALTY=1.059316
PRIMER_RIGHT_3_PENALTY=0.595208
PRIMER_INTERNAL_3_PENALTY=0.681621
PRIMER_LEFT_3_SEQUENCE=TCCTTCCAGGGAGATGAAGTT
PRIMER_RIGHT_3_SEQUENCE=CTGCAGAGGATCCTGAACCT
PRIMER_INTERNAL_3_SEQUENCE=CACCATGGCAAGTAGTGCTC
PRIMER_LEFT_3=33,21
PRIMER_RIGHT_3=123,20
PRIMER_INTERNAL_3=80,20
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=59.405
PRIMER_INTERNAL_3_TM=59.318
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=1.673877
PRIMER_LEFT_4_PENALTY=0.349041
PRIMER_RIGHT_4_PENALTY=1.324836
PRIMER_INTERNAL_4_PENALTY=4.073155
PRIMER_LEFT_4_SEQUENCE=GGTTCAGGATCCTCTGCAGA
PRIMER_RIGHT_4_SEQUENCE=TTTGTTGACATGTTGCTCCA
PRIMER_INTERNAL_4_SEQUENCE=CATCTAGGAAGACATCAGGCTTT
PRIMER_LEFT_4=105,20
PRIMER_RIGHT_4=187,20
PRIMER_INTERNAL_4=135,23
PRIMER_LEFT_4_TM=60.349
PRIMER_RIGHT_4_TM=58.675
PRIMER_INTERNAL_4_TM=58.927
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=8.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=83
=
SEQUENCE_ID=MH779
SEQUENCE_TEMPLATE=CTGAAGGTAAAACTTCTGATTATGTTTGAGAATTTAAATATTAAACGGACACAAATGACATTCTAAATACTTTCTCTTTCAGTTTTGACTTTATTTCTGACCTAATTTTCTATTTGACAAAACTTAGAGTGATTTATGGTGCTAAATCTCATTTAAAAAGAATAAATTCACTAAAGCAAGTTCCATACAAAAATCAGTATCACACATCTTGAGGGCTGTTACATCACTAG
SEQUENCE_INCLUDED_REGION=0,230
P3_COMMENT=CL is TGAGAATTTAAATATTAAACGGACA
P3_COMMENT=CR is TGATGTAACAGCCCTCAAGA
P3_COMMENT=CM is TTCAGTTTTGACTTTATTTCTGACC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.967474
PRIMER_LEFT_0_PENALTY=6.228722
PRIMER_RIGHT_0_PENALTY=0.738752
PRIMER_INTERNAL_0_PENALTY=4.869248
PRIMER_LEFT_0_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA
PRIMER_RIGHT_0_SEQUENCE=ACAGCCCTCAAGATGTGTGA
PRIMER_INTERNAL_0_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
PRIMER_LEFT_0=126,25
PRIMER_RIGHT_0=218,20
PRIMER_INTERNAL_0=172,24
PRIMER_LEFT_0_TM=58.771
PRIMER_RIGHT_0_TM=59.261
PRIMER_INTERNAL_0_TM=59.131
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=5.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=93
PRIMER_PAIR_1_PENALTY=7.230111
PRIMER_LEFT_1_PENALTY=6.491359
PRIMER_RIGHT_1_PENALTY=0.738752
PRIMER_INTERNAL_1_PENALTY=4.869248
PRIMER_LEFT_1_SEQUENCE=TGGTGCTAAATCTCATTTAAAAAGA
PRIMER_RIGHT_1_SEQUENCE=ACAGCCCTCAAGATGTGTGA
PRIMER_INTERNAL_1_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
PRIMER_LEFT_1=136,25
PRIMER_RIGHT_1=218,20
PRIMER_INTERNAL_1=172,24
PRIMER_LEFT_1_TM=58.509
PRIMER_RIGHT_1_TM=59.261
PRIMER_INTERNAL_1_TM=59.131
PRIMER_LEFT_1_GC_PERCENT=28.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=29.167
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=7.494337
PRIMER_LEFT_2_PENALTY=6.755585
PRIMER_RIGHT_2_PENALTY=0.738752
PRIMER_INTERNAL_2_PENALTY=4.869248
PRIMER_LEFT_2_SEQUENCE=TGATTTATGGTGCTAAATCTCATTT
PRIMER_RIGHT_2_SEQUENCE=ACAGCCCTCAAGATGTGTGA
PRIMER_INTERNAL_2_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
PRIMER_LEFT_2=129,25
PRIMER_RIGHT_2=218,20
PRIMER_INTERNAL_2=172,24
PRIMER_LEFT_2_TM=58.244
PRIMER_RIGHT_2_TM=59.261
PRIMER_INTERNAL_2_TM=59.131
PRIMER_LEFT_2_GC_PERCENT=28.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=7.504078
PRIMER_LEFT_3_PENALTY=6.228722
PRIMER_RIGHT_3_PENALTY=1.275356
PRIMER_INTERNAL_3_PENALTY=4.869248
PRIMER_LEFT_3_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA
PRIMER_RIGHT_3_SEQUENCE=AACAGCCCTCAAGATGTGTG
PRIMER_INTERNAL_3_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
PRIMER_LEFT_3=126,25
PRIMER_RIGHT_3=219,20
PRIMER_INTERNAL_3=172,24
PRIMER_LEFT_3_TM=58.771
PRIMER_RIGHT_3_TM=58.725
PRIMER_INTERNAL_3_TM=59.131
PRIMER_LEFT_3_GC_PERCENT=36.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=7.557640
PRIMER_LEFT_4_PENALTY=6.228722
PRIMER_RIGHT_4_PENALTY=1.328918
PRIMER_INTERNAL_4_PENALTY=4.869248
PRIMER_LEFT_4_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA
PRIMER_RIGHT_4_SEQUENCE=CAGCCCTCAAGATGTGTGAT
PRIMER_INTERNAL_4_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA
PRIMER_LEFT_4=126,25
PRIMER_RIGHT_4=217,20
PRIMER_INTERNAL_4=172,24
PRIMER_LEFT_4_TM=58.771
PRIMER_RIGHT_4_TM=58.671
PRIMER_INTERNAL_4_TM=59.131
PRIMER_LEFT_4_GC_PERCENT=36.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=29.167
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH780
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAATAAATCTTGAAATCGGGCAGTATAAGCCCTCCCATTTTGTNCTCTTTTTCAAAGTTCTTTGGCTGGTTTATTTCCTCGGTATCTTCATACAAATTCTAGAATTAGATTAGTTCAGCTTTTGGTTGGGGTTGCACTGAATTGATAGATCACTTGTGGAGGTTGGTATCTTGTTAAAGGAAGTGCTTGGAGGGTACGGTCTAACTTTGTTCCTGAATTTAGGGGTAAACATTCAGTTTTCACCCTTGTGTATACTGCTAG
SEQUENCE_INCLUDED_REGION=36,196
P3_COMMENT=CL is TGAAATCGGGCAGTATAAGC
P3_COMMENT=CR is TCTATCAATTCAGTGCAACCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.376191
PRIMER_LEFT_0_PENALTY=0.067187
PRIMER_RIGHT_0_PENALTY=1.309004
PRIMER_INTERNAL_0_PENALTY=8.521764
PRIMER_LEFT_0_SEQUENCE=TTGGCTGGTTTATTTCCTCG
PRIMER_RIGHT_0_SEQUENCE=ATCAATTCAGTGCAACCCCA
PRIMER_INTERNAL_0_SEQUENCE=AGAATTAGATTAGTTCAGCTTTTGGT
PRIMER_LEFT_0=102,20
PRIMER_RIGHT_0=186,20
PRIMER_INTERNAL_0=141,26
PRIMER_LEFT_0_TM=60.067
PRIMER_RIGHT_0_TM=61.309
PRIMER_INTERNAL_0_TM=57.478
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=30.769
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=1.567730
PRIMER_LEFT_1_PENALTY=0.067187
PRIMER_RIGHT_1_PENALTY=1.500543
PRIMER_INTERNAL_1_PENALTY=9.322509
PRIMER_LEFT_1_SEQUENCE=TTGGCTGGTTTATTTCCTCG
PRIMER_RIGHT_1_SEQUENCE=CAATTCAGTGCAACCCCAA
PRIMER_INTERNAL_1_SEQUENCE=CTAGAATTAGATTAGTTCAGCTTTTGG
PRIMER_LEFT_1=102,20
PRIMER_RIGHT_1=184,19
PRIMER_INTERNAL_1=139,27
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=60.501
PRIMER_INTERNAL_1_TM=57.677
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.368
PRIMER_INTERNAL_1_GC_PERCENT=33.333
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=1.623955
PRIMER_LEFT_2_PENALTY=0.067187
PRIMER_RIGHT_2_PENALTY=1.556768
PRIMER_INTERNAL_2_PENALTY=8.211922
PRIMER_LEFT_2_SEQUENCE=TTGGCTGGTTTATTTCCTCG
PRIMER_RIGHT_2_SEQUENCE=CTATCAATTCAGTGCAACCCC
PRIMER_INTERNAL_2_SEQUENCE=GAATTAGATTAGTTCAGCTTTTGGTT
PRIMER_LEFT_2=102,20
PRIMER_RIGHT_2=188,21
PRIMER_INTERNAL_2=142,26
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.443
PRIMER_INTERNAL_2_TM=57.788
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=30.769
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=10.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=1.628601
PRIMER_LEFT_3_PENALTY=0.319597
PRIMER_RIGHT_3_PENALTY=1.309004
PRIMER_INTERNAL_3_PENALTY=8.521764
PRIMER_LEFT_3_SEQUENCE=GGCTGGTTTATTTCCTCGGT
PRIMER_RIGHT_3_SEQUENCE=ATCAATTCAGTGCAACCCCA
PRIMER_INTERNAL_3_SEQUENCE=AGAATTAGATTAGTTCAGCTTTTGGT
PRIMER_LEFT_3=104,20
PRIMER_RIGHT_3=186,20
PRIMER_INTERNAL_3=141,26
PRIMER_LEFT_3_TM=60.320
PRIMER_RIGHT_3_TM=61.309
PRIMER_INTERNAL_3_TM=57.478
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=1.644426
PRIMER_LEFT_4_PENALTY=0.067187
PRIMER_RIGHT_4_PENALTY=1.577239
PRIMER_INTERNAL_4_PENALTY=8.211922
PRIMER_LEFT_4_SEQUENCE=TTGGCTGGTTTATTTCCTCG
PRIMER_RIGHT_4_SEQUENCE=TATCAATTCAGTGCAACCCC
PRIMER_INTERNAL_4_SEQUENCE=GAATTAGATTAGTTCAGCTTTTGGTT
PRIMER_LEFT_4=102,20
PRIMER_RIGHT_4=187,20
PRIMER_INTERNAL_4=142,26
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=58.423
PRIMER_INTERNAL_4_TM=57.788
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=30.769
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=10.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH781
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGCTATTTATTAGTAATATAAATGGTCAATGTATGCATTCATTATGCACCAAATAATAACTTCTGTACATACATTATTGTATTTCATTATCACAAAATTATGAGTGAGGGATGATTGTTATCCCTATTTTACAGATGAGAACACTGAGACTTAGAAGAAGTATCTTTCCCAAAGTCACAAAGTTAGTGACAGAGCCGGGATTCGAATCCATCAACTTGAATCCAGAGAAAATGTTCTGCATCACTGTACAACACTGACTCCTTTTTCTCCTTTGAAAACAAGGCAAGATGCA
SEQUENCE_INCLUDED_REGION=34,292
P3_COMMENT=CL is TGCATTCATTATGCACCAAA
P3_COMMENT=CR is TTGCCTTGTTTTCAAAGGAG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.524661
PRIMER_LEFT_0_PENALTY=1.145399
PRIMER_RIGHT_0_PENALTY=0.379262
PRIMER_INTERNAL_0_PENALTY=2.248900
PRIMER_LEFT_0_SEQUENCE=GGGATTCGAATCCATCAACTT
PRIMER_RIGHT_0_SEQUENCE=TGCATCTTGCCTTGTTTTCA
PRIMER_INTERNAL_0_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
PRIMER_LEFT_0=230,21
PRIMER_RIGHT_0=325,20
PRIMER_INTERNAL_0=255,22
PRIMER_LEFT_0_TM=60.145
PRIMER_RIGHT_0_TM=60.379
PRIMER_INTERNAL_0_TM=60.249
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=1.589777
PRIMER_LEFT_1_PENALTY=1.210515
PRIMER_RIGHT_1_PENALTY=0.379262
PRIMER_INTERNAL_1_PENALTY=2.248900
PRIMER_LEFT_1_SEQUENCE=GGGATTCGAATCCATCAACT
PRIMER_RIGHT_1_SEQUENCE=TGCATCTTGCCTTGTTTTCA
PRIMER_INTERNAL_1_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
PRIMER_LEFT_1=230,20
PRIMER_RIGHT_1=325,20
PRIMER_INTERNAL_1=255,22
PRIMER_LEFT_1_TM=58.789
PRIMER_RIGHT_1_TM=60.379
PRIMER_INTERNAL_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=2.002056
PRIMER_LEFT_2_PENALTY=1.622794
PRIMER_RIGHT_2_PENALTY=0.379262
PRIMER_INTERNAL_2_PENALTY=3.575181
PRIMER_LEFT_2_SEQUENCE=CGAATCCATCAACTTGAATCC
PRIMER_RIGHT_2_SEQUENCE=TGCATCTTGCCTTGTTTTCA
PRIMER_INTERNAL_2_SEQUENCE=TCTGCATCACTGTACAACACTGA
PRIMER_LEFT_2=236,21
PRIMER_RIGHT_2=325,20
PRIMER_INTERNAL_2=268,23
PRIMER_LEFT_2_TM=59.377
PRIMER_RIGHT_2_TM=60.379
PRIMER_INTERNAL_2_TM=59.425
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=7.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=2.002056
PRIMER_LEFT_3_PENALTY=1.622794
PRIMER_RIGHT_3_PENALTY=0.379262
PRIMER_INTERNAL_3_PENALTY=2.248900
PRIMER_LEFT_3_SEQUENCE=GGATTCGAATCCATCAACTTG
PRIMER_RIGHT_3_SEQUENCE=TGCATCTTGCCTTGTTTTCA
PRIMER_INTERNAL_3_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
PRIMER_LEFT_3=231,21
PRIMER_RIGHT_3=325,20
PRIMER_INTERNAL_3=255,22
PRIMER_LEFT_3_TM=59.377
PRIMER_RIGHT_3_TM=60.379
PRIMER_INTERNAL_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=2.250790
PRIMER_LEFT_4_PENALTY=1.145399
PRIMER_RIGHT_4_PENALTY=1.105391
PRIMER_INTERNAL_4_PENALTY=2.248900
PRIMER_LEFT_4_SEQUENCE=GGGATTCGAATCCATCAACTT
PRIMER_RIGHT_4_SEQUENCE=GCATCTTGCCTTGTTTTCAA
PRIMER_INTERNAL_4_SEQUENCE=CCAGAGAAAATGTTCTGCATCA
PRIMER_LEFT_4=230,21
PRIMER_RIGHT_4=324,20
PRIMER_INTERNAL_4=255,22
PRIMER_LEFT_4_TM=60.145
PRIMER_RIGHT_4_TM=58.895
PRIMER_INTERNAL_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=12.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH787
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGAACCCACTGACTATAAGGCAGCCTCACTCCTAGGAGGAACATCACAGCAACTCGCTAGCAATTGTAAAGTGTAATGATTTTCCCAGTCCTTCCCACCAAAATCTATGCCACTGACTCAGGGGGCTACACACTGAGAAGATGGAAATACCCAGACATTTCAAGGACTACTGGACACAGGCAGGAGTTGAATTTGACACTTGGGGAACCAATGCATCCTCATGGCTCCCCTGCTAAAATGGTAG
SEQUENCE_INCLUDED_REGION=36,248
P3_COMMENT=CL is GGAACCCACTGACTATAAGGC
P3_COMMENT=CR is CCCCAAGTGTCAAATTCAAC
P3_COMMENT=CM is GCCTCACTCCTAGGAGGAAC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.383705
PRIMER_LEFT_0_PENALTY=0.173836
PRIMER_RIGHT_0_PENALTY=0.209870
PRIMER_INTERNAL_0_PENALTY=0.243525
PRIMER_LEFT_0_SEQUENCE=ACTCAGGGGGCTACACACTG
PRIMER_RIGHT_0_SEQUENCE=TGGTTCCCCAAGTGTCAAAT
PRIMER_INTERNAL_0_SEQUENCE=ACTGGACACAGGCAGGAGTT
PRIMER_LEFT_0=155,20
PRIMER_RIGHT_0=249,20
PRIMER_INTERNAL_0=208,20
PRIMER_LEFT_0_TM=60.174
PRIMER_RIGHT_0_TM=60.210
PRIMER_INTERNAL_0_TM=59.756
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=0.383705
PRIMER_LEFT_1_PENALTY=0.173836
PRIMER_RIGHT_1_PENALTY=0.209870
PRIMER_INTERNAL_1_PENALTY=0.243525
PRIMER_LEFT_1_SEQUENCE=ACTCAGGGGGCTACACACTG
PRIMER_RIGHT_1_SEQUENCE=ATTGGTTCCCCAAGTGTCAA
PRIMER_INTERNAL_1_SEQUENCE=ACTGGACACAGGCAGGAGTT
PRIMER_LEFT_1=155,20
PRIMER_RIGHT_1=251,20
PRIMER_INTERNAL_1=208,20
PRIMER_LEFT_1_TM=60.174
PRIMER_RIGHT_1_TM=60.210
PRIMER_INTERNAL_1_TM=59.756
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=0.398177
PRIMER_LEFT_2_PENALTY=0.224341
PRIMER_RIGHT_2_PENALTY=0.173836
PRIMER_INTERNAL_2_PENALTY=0.168650
PRIMER_LEFT_2_SEQUENCE=CAGCAACTCGCTAGCAATTG
PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTAGCCCCCTGAGT
PRIMER_INTERNAL_2_SEQUENCE=ATTTTCCCAGTCCTTCCCAC
PRIMER_LEFT_2=86,20
PRIMER_RIGHT_2=174,20
PRIMER_INTERNAL_2=118,20
PRIMER_LEFT_2_TM=59.776
PRIMER_RIGHT_2_TM=60.174
PRIMER_INTERNAL_2_TM=60.169
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=8.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=0.403485
PRIMER_LEFT_3_PENALTY=0.229650
PRIMER_RIGHT_3_PENALTY=0.173836
PRIMER_INTERNAL_3_PENALTY=0.168650
PRIMER_LEFT_3_SEQUENCE=ATCACAGCAACTCGCTAGCA
PRIMER_RIGHT_3_SEQUENCE=CAGTGTGTAGCCCCCTGAGT
PRIMER_INTERNAL_3_SEQUENCE=ATTTTCCCAGTCCTTCCCAC
PRIMER_LEFT_3=82,20
PRIMER_RIGHT_3=174,20
PRIMER_INTERNAL_3=118,20
PRIMER_LEFT_3_TM=59.770
PRIMER_RIGHT_3_TM=60.174
PRIMER_INTERNAL_3_TM=60.169
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=93
PRIMER_PAIR_4_PENALTY=0.403485
PRIMER_LEFT_4_PENALTY=0.229650
PRIMER_RIGHT_4_PENALTY=0.173836
PRIMER_INTERNAL_4_PENALTY=0.168650
PRIMER_LEFT_4_SEQUENCE=CATCACAGCAACTCGCTAGC
PRIMER_RIGHT_4_SEQUENCE=CAGTGTGTAGCCCCCTGAGT
PRIMER_INTERNAL_4_SEQUENCE=ATTTTCCCAGTCCTTCCCAC
PRIMER_LEFT_4=81,20
PRIMER_RIGHT_4=174,20
PRIMER_INTERNAL_4=118,20
PRIMER_LEFT_4_TM=59.770
PRIMER_RIGHT_4_TM=60.174
PRIMER_INTERNAL_4_TM=60.169
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=94
=
SEQUENCE_ID=MH790
SEQUENCE_TEMPLATE=GGGAAGGTGTGGGGGGCTGCTGGGGCACAGTCATGGGGAACNCCAGTCCCCCTCTCCATGTGCTTCCCTCAATGCTCCCTGATGCCAGCAGTNCTGCCCCCGAACAGAAAGGGGCATTCCTGTGAGTTCCTTGTGTGGGGGACTGGTCACAGAGACTCCCCAAGTNCAGGGCAGGGTGGAGGGAGGCTGAGGGGTGGTTGAATGGACAGGAGAAGAG
SEQUENCE_INCLUDED_REGION=0,217
P3_COMMENT=CL is CTGCTGGGGCACAGTCAT
P3_COMMENT=CR is TCTTCTCCTGTCCATTCAAC
P3_COMMENT=CM is CCAGTCCCCCTCTCCATGT
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.874297
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=0.801345
PRIMER_INTERNAL_0_PENALTY=0.442898
PRIMER_LEFT_0_SEQUENCE=CTCCATGTGCTTCCCTCAAT
PRIMER_RIGHT_0_SEQUENCE=AGTCCCCCACACAAGGAACT
PRIMER_INTERNAL_0_SEQUENCE=GAACAGAAAGGGGCATTCCT
PRIMER_LEFT_0=53,20
PRIMER_RIGHT_0=143,20
PRIMER_INTERNAL_0=101,20
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=60.801
PRIMER_INTERNAL_0_TM=60.443
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=0.925391
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.852440
PRIMER_INTERNAL_1_PENALTY=2.063598
PRIMER_LEFT_1_SEQUENCE=CTCCATGTGCTTCCCTCAAT
PRIMER_RIGHT_1_SEQUENCE=CCACACAAGGAACTCACAGG
PRIMER_INTERNAL_1_SEQUENCE=CTCCCTGATGCCAGCAGT
PRIMER_LEFT_1=53,20
PRIMER_RIGHT_1=137,20
PRIMER_INTERNAL_1=74,18
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=59.148
PRIMER_INTERNAL_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=0.925391
PRIMER_LEFT_2_PENALTY=0.072951
PRIMER_RIGHT_2_PENALTY=0.852440
PRIMER_INTERNAL_2_PENALTY=1.587668
PRIMER_LEFT_2_SEQUENCE=CTCCATGTGCTTCCCTCAAT
PRIMER_RIGHT_2_SEQUENCE=CCCACACAAGGAACTCACAG
PRIMER_INTERNAL_2_SEQUENCE=CGAACAGAAAGGGGCATTC
PRIMER_LEFT_2=53,20
PRIMER_RIGHT_2=138,20
PRIMER_INTERNAL_2=100,19
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=59.148
PRIMER_INTERNAL_2_TM=60.588
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=52.632
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=0.932164
PRIMER_LEFT_3_PENALTY=0.072951
PRIMER_RIGHT_3_PENALTY=0.859212
PRIMER_INTERNAL_3_PENALTY=2.063598
PRIMER_LEFT_3_SEQUENCE=CTCCATGTGCTTCCCTCAAT
PRIMER_RIGHT_3_SEQUENCE=AAGGAACTCACAGGAATGCC
PRIMER_INTERNAL_3_SEQUENCE=CTCCCTGATGCCAGCAGT
PRIMER_LEFT_3=53,20
PRIMER_RIGHT_3=131,20
PRIMER_INTERNAL_3=74,18
PRIMER_LEFT_3_TM=60.073
PRIMER_RIGHT_3_TM=59.141
PRIMER_INTERNAL_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=0.955569
PRIMER_LEFT_4_PENALTY=0.072951
PRIMER_RIGHT_4_PENALTY=0.882618
PRIMER_INTERNAL_4_PENALTY=2.063598
PRIMER_LEFT_4_SEQUENCE=CTCCATGTGCTTCCCTCAAT
PRIMER_RIGHT_4_SEQUENCE=AACTCACAGGAATGCCCCTT
PRIMER_INTERNAL_4_SEQUENCE=CTCCCTGATGCCAGCAGT
PRIMER_LEFT_4=53,20
PRIMER_RIGHT_4=127,20
PRIMER_INTERNAL_4=74,18
PRIMER_LEFT_4_TM=60.073
PRIMER_RIGHT_4_TM=60.883
PRIMER_INTERNAL_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH795
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCAGGGNCAACCAGAAATTCATAGCACAGGAATGGCCGGCAGCAAGGGGTCTGAGAATGTCAAAACAAGCATCCTGGAGGGAAGGCAGATGCCATCAGACTTCTCCAAAGACTCTTTGTACAGACAGTATTTCAGACCATCCCAGGCATCAACAGGAAGTGAGTAGTTACCTTCTGATTGCACGTATAAGGTGACTGGAATACAAAATGCTGGGTTTTTGGCCCGAGGNCCCATGAAGAGATCATAGTTCAAATTTCAACATGTCTAAATTCAGTTCTAGCCACTATGGCTTGCTAGTGTTACCTCAAAATAGAGACATTTATTTTCCCTAA
SEQUENCE_INCLUDED_REGION=36,334
P3_COMMENT=CL is ATTCATAGCACAGGAATGGC
P3_COMMENT=CR is AGGGAAAATAAATGTCTCTATTTTG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.267394
PRIMER_LEFT_0_PENALTY=0.106310
PRIMER_RIGHT_0_PENALTY=0.161083
PRIMER_INTERNAL_0_PENALTY=1.790576
PRIMER_LEFT_0_SEQUENCE=TATTTCAGACCATCCCAGGC
PRIMER_RIGHT_0_SEQUENCE=CCAAAAACCCAGCATTTTGT
PRIMER_INTERNAL_0_SEQUENCE=TGCACGTATAAGGTGACTGGA
PRIMER_LEFT_0=165,20
PRIMER_RIGHT_0=258,20
PRIMER_INTERNAL_0=216,21
PRIMER_LEFT_0_TM=59.894
PRIMER_RIGHT_0_TM=59.839
PRIMER_INTERNAL_0_TM=59.209
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=94
PRIMER_PAIR_1_PENALTY=0.330555
PRIMER_LEFT_1_PENALTY=0.169471
PRIMER_RIGHT_1_PENALTY=0.161083
PRIMER_INTERNAL_1_PENALTY=1.790576
PRIMER_LEFT_1_SEQUENCE=CAGGCATCAACAGGAAGTGA
PRIMER_RIGHT_1_SEQUENCE=CCAAAAACCCAGCATTTTGT
PRIMER_INTERNAL_1_SEQUENCE=TGCACGTATAAGGTGACTGGA
PRIMER_LEFT_1=180,20
PRIMER_RIGHT_1=258,20
PRIMER_INTERNAL_1=216,21
PRIMER_LEFT_1_TM=59.831
PRIMER_RIGHT_1_TM=59.839
PRIMER_INTERNAL_1_TM=59.209
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=0.335917
PRIMER_LEFT_2_PENALTY=0.166445
PRIMER_RIGHT_2_PENALTY=0.169471
PRIMER_INTERNAL_2_PENALTY=5.792857
PRIMER_LEFT_2_SEQUENCE=AGGCAGATGCCATCAGACTT
PRIMER_RIGHT_2_SEQUENCE=TCACTTCCTGTTGATGCCTG
PRIMER_INTERNAL_2_SEQUENCE=ACAGACAGTATTTCAGACCATCC
PRIMER_LEFT_2=120,20
PRIMER_RIGHT_2=199,20
PRIMER_INTERNAL_2=157,23
PRIMER_LEFT_2_TM=59.834
PRIMER_RIGHT_2_TM=59.831
PRIMER_INTERNAL_2_TM=57.207
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=9.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=0.415032
PRIMER_LEFT_3_PENALTY=0.308722
PRIMER_RIGHT_3_PENALTY=0.106310
PRIMER_INTERNAL_3_PENALTY=0.166445
PRIMER_LEFT_3_SEQUENCE=TGTCAAAACAAGCATCCTGG
PRIMER_RIGHT_3_SEQUENCE=GCCTGGGATGGTCTGAAATA
PRIMER_INTERNAL_3_SEQUENCE=AGGCAGATGCCATCAGACTT
PRIMER_LEFT_3=95,20
PRIMER_RIGHT_3=184,20
PRIMER_INTERNAL_3=120,20
PRIMER_LEFT_3_TM=59.691
PRIMER_RIGHT_3_TM=59.894
PRIMER_INTERNAL_3_TM=59.834
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=9.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=0.732726
PRIMER_LEFT_4_PENALTY=0.626415
PRIMER_RIGHT_4_PENALTY=0.106310
PRIMER_INTERNAL_4_PENALTY=0.166445
PRIMER_LEFT_4_SEQUENCE=CAAAACAAGCATCCTGGAGG
PRIMER_RIGHT_4_SEQUENCE=GCCTGGGATGGTCTGAAATA
PRIMER_INTERNAL_4_SEQUENCE=AGGCAGATGCCATCAGACTT
PRIMER_LEFT_4=98,20
PRIMER_RIGHT_4=184,20
PRIMER_INTERNAL_4=120,20
PRIMER_LEFT_4_TM=60.626
PRIMER_RIGHT_4_TM=59.894
PRIMER_INTERNAL_4_TM=59.834
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=9.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH799
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTGCAGCATTCCATAATGTGAATATATAACACTTTATTCAAAATTTGGGGAAATAGTACCTTGTACATACATAATTTCACATGTTTGCCAGTGTGTGTTTTAGATACATGCCTGGAAGTGAAATTGCAGGTAGCCTGCATATGTTAATTTTGCTAGGTATTACCAAACCAG
SEQUENCE_INCLUDED_REGION=36,173
P3_COMMENT=CL is CTGTGCAGCATTCCATAATG
P3_COMMENT=CR is AACATATGCAGGCTACCTGC
P3_COMMENT=CM is ATAACACTTTATTCAAAATTTGGGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.472484
PRIMER_LEFT_0_PENALTY=1.088344
PRIMER_RIGHT_0_PENALTY=0.384141
PRIMER_INTERNAL_0_PENALTY=3.718759
PRIMER_LEFT_0_SEQUENCE=TGCAGCATTCCATAATGTGAA
PRIMER_RIGHT_0_SEQUENCE=CACACACTGGCAAACATGTG
PRIMER_INTERNAL_0_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
PRIMER_LEFT_0=39,21
PRIMER_RIGHT_0=134,20
PRIMER_INTERNAL_0=67,23
PRIMER_LEFT_0_TM=60.088
PRIMER_RIGHT_0_TM=59.616
PRIMER_INTERNAL_0_TM=59.281
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=30.435
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=7.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=96
PRIMER_PAIR_1_PENALTY=1.733889
PRIMER_LEFT_1_PENALTY=1.349748
PRIMER_RIGHT_1_PENALTY=0.384141
PRIMER_INTERNAL_1_PENALTY=3.718759
PRIMER_LEFT_1_SEQUENCE=TGCAGCATTCCATAATGTGA
PRIMER_RIGHT_1_SEQUENCE=CACACACTGGCAAACATGTG
PRIMER_INTERNAL_1_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
PRIMER_LEFT_1=39,20
PRIMER_RIGHT_1=134,20
PRIMER_INTERNAL_1=67,23
PRIMER_LEFT_1_TM=58.650
PRIMER_RIGHT_1_TM=59.616
PRIMER_INTERNAL_1_TM=59.281
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=30.435
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=1.821598
PRIMER_LEFT_2_PENALTY=1.437458
PRIMER_RIGHT_2_PENALTY=0.384141
PRIMER_INTERNAL_2_PENALTY=3.718759
PRIMER_LEFT_2_SEQUENCE=GTGCAGCATTCCATAATGTGA
PRIMER_RIGHT_2_SEQUENCE=CACACACTGGCAAACATGTG
PRIMER_INTERNAL_2_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
PRIMER_LEFT_2=38,21
PRIMER_RIGHT_2=134,20
PRIMER_INTERNAL_2=67,23
PRIMER_LEFT_2_TM=59.563
PRIMER_RIGHT_2_TM=59.616
PRIMER_INTERNAL_2_TM=59.281
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=30.435
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=97
PRIMER_PAIR_3_PENALTY=1.922620
PRIMER_LEFT_3_PENALTY=1.538479
PRIMER_RIGHT_3_PENALTY=0.384141
PRIMER_INTERNAL_3_PENALTY=3.718759
PRIMER_LEFT_3_SEQUENCE=TGTGCAGCATTCCATAATGTG
PRIMER_RIGHT_3_SEQUENCE=CACACACTGGCAAACATGTG
PRIMER_INTERNAL_3_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
PRIMER_LEFT_3=37,21
PRIMER_RIGHT_3=134,20
PRIMER_INTERNAL_3=67,23
PRIMER_LEFT_3_TM=60.538
PRIMER_RIGHT_3_TM=59.616
PRIMER_INTERNAL_3_TM=59.281
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=30.435
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=1.951637
PRIMER_LEFT_4_PENALTY=1.088344
PRIMER_RIGHT_4_PENALTY=0.863293
PRIMER_INTERNAL_4_PENALTY=3.718759
PRIMER_LEFT_4_SEQUENCE=TGCAGCATTCCATAATGTGAA
PRIMER_RIGHT_4_SEQUENCE=CACACTGGCAAACATGTGAA
PRIMER_INTERNAL_4_SEQUENCE=CACTTTATTCAAAATTTGGGGAA
PRIMER_LEFT_4=39,21
PRIMER_RIGHT_4=132,20
PRIMER_INTERNAL_4=67,23
PRIMER_LEFT_4_TM=60.088
PRIMER_RIGHT_4_TM=59.137
PRIMER_INTERNAL_4_TM=59.281
PRIMER_LEFT_4_GC_PERCENT=38.095
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=30.435
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=7.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=94
=
SEQUENCE_ID=MH800
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGCTTTTTGTTGTTATTGTCAGTTTGTAACAAACACCATCCTGCTTAGTNTGCACCCTACACTGCTCTAAGCACCTTAATAGGAATGCATTTAATCATTGTAAAAATCCTGGGATTCTATTGCAGGCTTAGGTGACTCAAAAGCCACTCTTTCCACGACAACCCTATTAAAACAAGAAATGTGAATAAGCCATGTTGTTGCTATGCGCTTAGTGAGTCCTCAGTAAATGTTAG
SEQUENCE_INCLUDED_REGION=34,234
P3_COMMENT=CL is TTATTGTCAGTTTGTAACAAACACC
P3_COMMENT=CR is CTAAGCGCATAGCAACAACA
P3_COMMENT=CM is TGCACCCTACACTGCTCTAA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.507626
PRIMER_LEFT_0_PENALTY=0.409420
PRIMER_RIGHT_0_PENALTY=0.098207
PRIMER_INTERNAL_0_PENALTY=0.545130
PRIMER_LEFT_0_SEQUENCE=TGCAGGCTTAGGTGACTCAA
PRIMER_RIGHT_0_SEQUENCE=AAGCGCATAGCAACAACATG
PRIMER_INTERNAL_0_SEQUENCE=CACTCTTTCCACGACAACCC
PRIMER_LEFT_0=155,20
PRIMER_RIGHT_0=244,20
PRIMER_INTERNAL_0=179,20
PRIMER_LEFT_0_TM=59.591
PRIMER_RIGHT_0_TM=59.902
PRIMER_INTERNAL_0_TM=60.545
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=0.507626
PRIMER_LEFT_1_PENALTY=0.409420
PRIMER_RIGHT_1_PENALTY=0.098207
PRIMER_INTERNAL_1_PENALTY=0.545130
PRIMER_LEFT_1_SEQUENCE=TTGCAGGCTTAGGTGACTCA
PRIMER_RIGHT_1_SEQUENCE=AAGCGCATAGCAACAACATG
PRIMER_INTERNAL_1_SEQUENCE=CACTCTTTCCACGACAACCC
PRIMER_LEFT_1=154,20
PRIMER_RIGHT_1=244,20
PRIMER_INTERNAL_1=179,20
PRIMER_LEFT_1_TM=59.591
PRIMER_RIGHT_1_TM=59.902
PRIMER_INTERNAL_1_TM=60.545
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.808075
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.660780
PRIMER_INTERNAL_2_PENALTY=2.692901
PRIMER_LEFT_2_SEQUENCE=AAAAGCCACTCTTTCCACGA
PRIMER_RIGHT_2_SEQUENCE=CTGAGGACTCACTAAGCGCA
PRIMER_INTERNAL_2_SEQUENCE=TGAATAAGCCATGTTGTTGCT
PRIMER_LEFT_2=173,20
PRIMER_RIGHT_2=257,20
PRIMER_INTERNAL_2=216,21
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=59.339
PRIMER_INTERNAL_2_TM=58.307
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=38.095
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=11.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=85
PRIMER_PAIR_3_PENALTY=1.205911
PRIMER_LEFT_3_PENALTY=0.545130
PRIMER_RIGHT_3_PENALTY=0.660780
PRIMER_INTERNAL_3_PENALTY=2.692901
PRIMER_LEFT_3_SEQUENCE=CACTCTTTCCACGACAACCC
PRIMER_RIGHT_3_SEQUENCE=CTGAGGACTCACTAAGCGCA
PRIMER_INTERNAL_3_SEQUENCE=TGAATAAGCCATGTTGTTGCT
PRIMER_LEFT_3=179,20
PRIMER_RIGHT_3=257,20
PRIMER_INTERNAL_3=216,21
PRIMER_LEFT_3_TM=60.545
PRIMER_RIGHT_3_TM=59.339
PRIMER_INTERNAL_3_TM=58.307
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=11.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=1.205911
PRIMER_LEFT_4_PENALTY=0.545130
PRIMER_RIGHT_4_PENALTY=0.660780
PRIMER_INTERNAL_4_PENALTY=2.692901
PRIMER_LEFT_4_SEQUENCE=CCACTCTTTCCACGACAACC
PRIMER_RIGHT_4_SEQUENCE=CTGAGGACTCACTAAGCGCA
PRIMER_INTERNAL_4_SEQUENCE=TGAATAAGCCATGTTGTTGCT
PRIMER_LEFT_4=178,20
PRIMER_RIGHT_4=257,20
PRIMER_INTERNAL_4=216,21
PRIMER_LEFT_4_TM=60.545
PRIMER_RIGHT_4_TM=59.339
PRIMER_INTERNAL_4_TM=58.307
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=11.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH803
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCTCCTCAGAGAGNATTTGCATTTGNCTTTTTCAAATCACTGGAGGAATTATTTATATCAGACTACTACAGTTTAATGCCACGCTTTAAACCACACTGGTCATGTGAATATTCTATTTAATTTTACATGATGCTTAAACTGTGCTTTTGAATATTCAAGGTATGTGTGTGTGCGCATGTGTATGTGTATATGTGTGTGTTTACATTAGCCACAAATTAAAGACAGATAAATTTTCTTGCTGACCACTTCTGTGCAATAGG
SEQUENCE_INCLUDED_REGION=36,264
P3_COMMENT=CL is ATCAGACTACTACAGTTTAATGCCA
P3_COMMENT=CR is ATTGCACAGAAGTGGTCAGC
P3_COMMENT=CM is ATTTTACATGATGCTTAAACTGTGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.239865
PRIMER_LEFT_0_PENALTY=0.175676
PRIMER_RIGHT_0_PENALTY=0.064190
PRIMER_INTERNAL_0_PENALTY=3.061184
PRIMER_LEFT_0_SEQUENCE=GCCACGCTTTAAACCACACT
PRIMER_RIGHT_0_SEQUENCE=ATGCGCACACACACATACCT
PRIMER_INTERNAL_0_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
PRIMER_LEFT_0=117,20
PRIMER_RIGHT_0=216,20
PRIMER_INTERNAL_0=167,23
PRIMER_LEFT_0_TM=60.176
PRIMER_RIGHT_0_TM=60.064
PRIMER_INTERNAL_0_TM=59.939
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.442004
PRIMER_LEFT_1_PENALTY=0.064190
PRIMER_RIGHT_1_PENALTY=0.377815
PRIMER_INTERNAL_1_PENALTY=3.926731
PRIMER_LEFT_1_SEQUENCE=AGGTATGTGTGTGTGCGCAT
PRIMER_RIGHT_1_SEQUENCE=GCACAGAAGTGGTCAGCAAG
PRIMER_INTERNAL_1_SEQUENCE=TGTGTGTGTTTACATTAGCCACA
PRIMER_LEFT_1=197,20
PRIMER_RIGHT_1=293,20
PRIMER_INTERNAL_1=230,23
PRIMER_LEFT_1_TM=60.064
PRIMER_RIGHT_1_TM=59.622
PRIMER_INTERNAL_1_TM=59.073
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=10.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=97
PRIMER_PAIR_2_PENALTY=0.823640
PRIMER_LEFT_2_PENALTY=0.759451
PRIMER_RIGHT_2_PENALTY=0.064190
PRIMER_INTERNAL_2_PENALTY=3.061184
PRIMER_LEFT_2_SEQUENCE=CACGCTTTAAACCACACTGG
PRIMER_RIGHT_2_SEQUENCE=ATGCGCACACACACATACCT
PRIMER_INTERNAL_2_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
PRIMER_LEFT_2=119,20
PRIMER_RIGHT_2=216,20
PRIMER_INTERNAL_2=167,23
PRIMER_LEFT_2_TM=59.241
PRIMER_RIGHT_2_TM=60.064
PRIMER_INTERNAL_2_TM=59.939
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=98
PRIMER_PAIR_3_PENALTY=0.823640
PRIMER_LEFT_3_PENALTY=0.759451
PRIMER_RIGHT_3_PENALTY=0.064190
PRIMER_INTERNAL_3_PENALTY=3.061184
PRIMER_LEFT_3_SEQUENCE=CCACGCTTTAAACCACACTG
PRIMER_RIGHT_3_SEQUENCE=ATGCGCACACACACATACCT
PRIMER_INTERNAL_3_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
PRIMER_LEFT_3=118,20
PRIMER_RIGHT_3=216,20
PRIMER_INTERNAL_3=167,23
PRIMER_LEFT_3_TM=59.241
PRIMER_RIGHT_3_TM=60.064
PRIMER_INTERNAL_3_TM=59.939
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=0.839027
PRIMER_LEFT_4_PENALTY=0.003166
PRIMER_RIGHT_4_PENALTY=0.835860
PRIMER_INTERNAL_4_PENALTY=3.061184
PRIMER_LEFT_4_SEQUENCE=AATGCCACGCTTTAAACCAC
PRIMER_RIGHT_4_SEQUENCE=CGCACACACACATACCTTGA
PRIMER_INTERNAL_4_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA
PRIMER_LEFT_4=114,20
PRIMER_RIGHT_4=213,20
PRIMER_INTERNAL_4=167,23
PRIMER_LEFT_4_TM=60.003
PRIMER_RIGHT_4_TM=59.164
PRIMER_INTERNAL_4_TM=59.939
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH805
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACCCAGAGCAAGGAAAGATCATGAAAACCCAAGCAGAAGCAAGACACTGGGTTCCAGCATCAATGCTAAGGGCTAGAACATTTTCTGATGTTTTGCTTGGAAAGGCAGAATTTGGTTGTCTAGTGCGTCCACTAATTGGCCTCCATCTGACACCCTGGAGGGATGGGCTGATTAGAAGCCACAGCTGCTTTGCTCATTATTTCTGATGAATGGACATCACCTGGCGTCTCCGGTGATGGCGCCAG
SEQUENCE_INCLUDED_REGION=34,248
P3_COMMENT=CL is AGATCATGAAAACCCAAGCA
P3_COMMENT=CR is CAGGTGATGTCCATTCATCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.183953
PRIMER_LEFT_0_PENALTY=0.006945
PRIMER_RIGHT_0_PENALTY=0.177008
PRIMER_INTERNAL_0_PENALTY=0.373535
PRIMER_LEFT_0_SEQUENCE=AAACCCAAGCAGAAGCAAGA
PRIMER_RIGHT_0_SEQUENCE=ATTCTGCCTTTCCAAGCAAA
PRIMER_INTERNAL_0_SEQUENCE=CAGCATCAATGCTAAGGGCT
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=147,20
PRIMER_INTERNAL_0=91,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=59.823
PRIMER_INTERNAL_0_TM=60.374
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=0.183953
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.177008
PRIMER_INTERNAL_1_PENALTY=0.373535
PRIMER_LEFT_1_SEQUENCE=AAACCCAAGCAGAAGCAAGA
PRIMER_RIGHT_1_SEQUENCE=AATTCTGCCTTTCCAAGCAA
PRIMER_INTERNAL_1_SEQUENCE=CAGCATCAATGCTAAGGGCT
PRIMER_LEFT_1=61,20
PRIMER_RIGHT_1=148,20
PRIMER_INTERNAL_1=91,20
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.823
PRIMER_INTERNAL_1_TM=60.374
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=0.183953
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=0.177008
PRIMER_INTERNAL_2_PENALTY=0.373535
PRIMER_LEFT_2_SEQUENCE=AAACCCAAGCAGAAGCAAGA
PRIMER_RIGHT_2_SEQUENCE=AAATTCTGCCTTTCCAAGCA
PRIMER_INTERNAL_2_SEQUENCE=CAGCATCAATGCTAAGGGCT
PRIMER_LEFT_2=61,20
PRIMER_RIGHT_2=149,20
PRIMER_INTERNAL_2=91,20
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.823
PRIMER_INTERNAL_2_TM=60.374
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=0.183953
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.177008
PRIMER_INTERNAL_3_PENALTY=0.373535
PRIMER_LEFT_3_SEQUENCE=AAACCCAAGCAGAAGCAAGA
PRIMER_RIGHT_3_SEQUENCE=CAAATTCTGCCTTTCCAAGC
PRIMER_INTERNAL_3_SEQUENCE=CAGCATCAATGCTAAGGGCT
PRIMER_LEFT_3=61,20
PRIMER_RIGHT_3=150,20
PRIMER_INTERNAL_3=91,20
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=59.823
PRIMER_INTERNAL_3_TM=60.374
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=0.192383
PRIMER_LEFT_4_PENALTY=0.177008
PRIMER_RIGHT_4_PENALTY=0.015375
PRIMER_INTERNAL_4_PENALTY=0.066262
PRIMER_LEFT_4_SEQUENCE=GCTTGGAAAGGCAGAATTTG
PRIMER_RIGHT_4_SEQUENCE=GCTGTGGCTTCTAATCAGCC
PRIMER_INTERNAL_4_SEQUENCE=AATTGGCCTCCATCTGACAC
PRIMER_LEFT_4=131,20
PRIMER_RIGHT_4=221,20
PRIMER_INTERNAL_4=170,20
PRIMER_LEFT_4_TM=59.823
PRIMER_RIGHT_4_TM=59.985
PRIMER_INTERNAL_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH806
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAACCTAATATGTACTCTGGGCTGACTTCCATGTTATAACTGTGTGCTTCAATGCTGGCCAAATTTAATCATGCTATAGTTTCCCTCACTTTTCAATTACATTTTTTATTTCCTTACTCTGGACCACCATGACTGCTAGAATTGTCTGCGTGCATTGTCCAAGCCAG
SEQUENCE_INCLUDED_REGION=36,171
P3_COMMENT=CL is TGGGCTGACTTCCATGTTAT
P3_COMMENT=CR is CAGACAATTCTAGCAGTCATGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.373173
PRIMER_LEFT_0_PENALTY=0.074231
PRIMER_RIGHT_0_PENALTY=0.298942
PRIMER_INTERNAL_0_PENALTY=4.650821
PRIMER_LEFT_0_SEQUENCE=CTTCAATGCTGGCCAAATTT
PRIMER_RIGHT_0_SEQUENCE=TAGCAGTCATGGTGGTCCAG
PRIMER_INTERNAL_0_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
PRIMER_LEFT_0=86,20
PRIMER_RIGHT_0=177,20
PRIMER_INTERNAL_0=111,24
PRIMER_LEFT_0_TM=60.074
PRIMER_RIGHT_0_TM=59.701
PRIMER_INTERNAL_0_TM=59.349
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=6.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=0.373173
PRIMER_LEFT_1_PENALTY=0.074231
PRIMER_RIGHT_1_PENALTY=0.298942
PRIMER_INTERNAL_1_PENALTY=4.650821
PRIMER_LEFT_1_SEQUENCE=CTTCAATGCTGGCCAAATTT
PRIMER_RIGHT_1_SEQUENCE=CTAGCAGTCATGGTGGTCCA
PRIMER_INTERNAL_1_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
PRIMER_LEFT_1=86,20
PRIMER_RIGHT_1=178,20
PRIMER_INTERNAL_1=111,24
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.701
PRIMER_INTERNAL_1_TM=59.349
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=0.394429
PRIMER_LEFT_2_PENALTY=0.095488
PRIMER_RIGHT_2_PENALTY=0.298942
PRIMER_INTERNAL_2_PENALTY=4.650821
PRIMER_LEFT_2_SEQUENCE=ACTGTGTGCTTCAATGCTGG
PRIMER_RIGHT_2_SEQUENCE=TAGCAGTCATGGTGGTCCAG
PRIMER_INTERNAL_2_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
PRIMER_LEFT_2=78,20
PRIMER_RIGHT_2=177,20
PRIMER_INTERNAL_2=111,24
PRIMER_LEFT_2_TM=59.905
PRIMER_RIGHT_2_TM=59.701
PRIMER_INTERNAL_2_TM=59.349
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=1.099900
PRIMER_LEFT_3_PENALTY=0.074231
PRIMER_RIGHT_3_PENALTY=1.025669
PRIMER_INTERNAL_3_PENALTY=4.650821
PRIMER_LEFT_3_SEQUENCE=CTTCAATGCTGGCCAAATTT
PRIMER_RIGHT_3_SEQUENCE=CATGGTGGTCCAGAGTAAGGA
PRIMER_INTERNAL_3_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
PRIMER_LEFT_3=86,20
PRIMER_RIGHT_3=170,21
PRIMER_INTERNAL_3=111,24
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.974
PRIMER_INTERNAL_3_TM=59.349
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=1.099900
PRIMER_LEFT_4_PENALTY=0.074231
PRIMER_RIGHT_4_PENALTY=1.025669
PRIMER_INTERNAL_4_PENALTY=4.650821
PRIMER_LEFT_4_SEQUENCE=CTTCAATGCTGGCCAAATTT
PRIMER_RIGHT_4_SEQUENCE=TCATGGTGGTCCAGAGTAAGG
PRIMER_INTERNAL_4_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA
PRIMER_LEFT_4=86,20
PRIMER_RIGHT_4=171,21
PRIMER_INTERNAL_4=111,24
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.974
PRIMER_INTERNAL_4_TM=59.349
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH813
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTAGCACTTGTTGGGCTAGAGGGTCATGGTGGTNGTNAGGGTAGTTAAATTTTTGTCATGTTTTGAAGTGTGAAGATTTTCCAACCTTCAAAGAATAATAAAGAAGGGAGTGCCCCTCCATAGGTCAAGCAAATGTACAATCACCCAGAGTNATAAAATATGTAGCCTTAGGTGATTGTCAAGTAATTCCCTATATAATTTTTATAAAGATGAGTTTCCCATTGGAACATTTATAAATGACTTATCTATAAAAATTCATTCACCGCACCAATCTTAGG
SEQUENCE_INCLUDED_REGION=35,279
P3_COMMENT=CL is ACTTGTTGGGCTAGAGGGTC
P3_COMMENT=CR is TGTTCCAATGGGAAACTCAT
P3_COMMENT=CM is TAAATTTTTGTCATGTTTTGAAGTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.659900
PRIMER_LEFT_0_PENALTY=0.246438
PRIMER_RIGHT_0_PENALTY=2.413463
PRIMER_INTERNAL_0_PENALTY=7.279304
PRIMER_LEFT_0_SEQUENCE=CTTGTTGGGCTAGAGGGTCA
PRIMER_RIGHT_0_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
PRIMER_INTERNAL_0_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
PRIMER_LEFT_0=42,20
PRIMER_RIGHT_0=125,22
PRIMER_INTERNAL_0=74,27
PRIMER_LEFT_0_TM=60.246
PRIMER_RIGHT_0_TM=59.587
PRIMER_INTERNAL_0_TM=60.279
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=36.364
PRIMER_INTERNAL_0_GC_PERCENT=29.630
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=2.863292
PRIMER_LEFT_1_PENALTY=0.449829
PRIMER_RIGHT_1_PENALTY=2.413463
PRIMER_INTERNAL_1_PENALTY=7.279304
PRIMER_LEFT_1_SEQUENCE=TTGTTGGGCTAGAGGGTCAT
PRIMER_RIGHT_1_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
PRIMER_INTERNAL_1_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
PRIMER_LEFT_1=43,20
PRIMER_RIGHT_1=125,22
PRIMER_INTERNAL_1=74,27
PRIMER_LEFT_1_TM=59.550
PRIMER_RIGHT_1_TM=59.587
PRIMER_INTERNAL_1_TM=60.279
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=36.364
PRIMER_INTERNAL_1_GC_PERCENT=29.630
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=6.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=2.917159
PRIMER_LEFT_2_PENALTY=0.503696
PRIMER_RIGHT_2_PENALTY=2.413463
PRIMER_INTERNAL_2_PENALTY=7.279304
PRIMER_LEFT_2_SEQUENCE=AGCACTTGTTGGGCTAGAGG
PRIMER_RIGHT_2_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
PRIMER_INTERNAL_2_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
PRIMER_LEFT_2=38,20
PRIMER_RIGHT_2=125,22
PRIMER_INTERNAL_2=74,27
PRIMER_LEFT_2_TM=59.496
PRIMER_RIGHT_2_TM=59.587
PRIMER_INTERNAL_2_TM=60.279
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=36.364
PRIMER_INTERNAL_2_GC_PERCENT=29.630
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=3.205698
PRIMER_LEFT_3_PENALTY=0.792236
PRIMER_RIGHT_3_PENALTY=2.413463
PRIMER_INTERNAL_3_PENALTY=7.279304
PRIMER_LEFT_3_SEQUENCE=CACTTGTTGGGCTAGAGGGT
PRIMER_RIGHT_3_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
PRIMER_INTERNAL_3_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
PRIMER_LEFT_3=40,20
PRIMER_RIGHT_3=125,22
PRIMER_INTERNAL_3=74,27
PRIMER_LEFT_3_TM=59.208
PRIMER_RIGHT_3_TM=59.587
PRIMER_INTERNAL_3_TM=60.279
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=36.364
PRIMER_INTERNAL_3_GC_PERCENT=29.630
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=86
PRIMER_PAIR_4_PENALTY=3.468418
PRIMER_LEFT_4_PENALTY=1.054956
PRIMER_RIGHT_4_PENALTY=2.413463
PRIMER_INTERNAL_4_PENALTY=7.279304
PRIMER_LEFT_4_SEQUENCE=TGTTGGGCTAGAGGGTCATG
PRIMER_RIGHT_4_SEQUENCE=TGAAGGTTGGAAAATCTTCACA
PRIMER_INTERNAL_4_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG
PRIMER_LEFT_4=44,20
PRIMER_RIGHT_4=125,22
PRIMER_INTERNAL_4=74,27
PRIMER_LEFT_4_TM=61.055
PRIMER_RIGHT_4_TM=59.587
PRIMER_INTERNAL_4_TM=60.279
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=36.364
PRIMER_INTERNAL_4_GC_PERCENT=29.630
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH820
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT
SEQUENCE_INCLUDED_REGION=36,33
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH822
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTGTATATTCCTGAACTCTGTAGACCTTCCTGACCTGTTTGCAGTGGATACTGTTTTTGAAGGCTCTGTCTCAGTATCTGAAGTTTTTGTCTCCAGAGAAGTCATTTTGTAGGTGTTCCTGGGCGTTTTTGCTACGTTTCCATTTTCTCTAATACACTGCCGTCTTAAGGGAGGGCTTGCAGAGCATTTATCAGATGGCTGTTTTGCTGCATTCTGTGCACTGAAGAACACATTTCCTCCTGAAATAAAAACATACACAATAAAAACATTCTATTAGTGTCTCATGTCAATCA
SEQUENCE_INCLUDED_REGION=36,296
P3_COMMENT=CL is CTTCCTGACCTGTTTGCAGT
P3_COMMENT=CR is CTTCAGTGCACAGAATGCAG
P3_COMMENT=CM is GATACTGTTTTTGAAGGCTCTGTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.156350
PRIMER_LEFT_0_PENALTY=0.127097
PRIMER_RIGHT_0_PENALTY=0.029253
PRIMER_INTERNAL_0_PENALTY=2.030276
PRIMER_LEFT_0_SEQUENCE=TTCCTGACCTGTTTGCAGTG
PRIMER_RIGHT_0_SEQUENCE=GCCCAGGAACACCTACAAAA
PRIMER_INTERNAL_0_SEQUENCE=TGTTTTTGAAGGCTCTGTCTCA
PRIMER_LEFT_0=65,20
PRIMER_RIGHT_0=162,20
PRIMER_INTERNAL_0=90,22
PRIMER_LEFT_0_TM=59.873
PRIMER_RIGHT_0_TM=59.971
PRIMER_INTERNAL_0_TM=60.030
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=0.216225
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.186972
PRIMER_INTERNAL_1_PENALTY=1.621453
PRIMER_LEFT_1_SEQUENCE=TTTTGTAGGTGTTCCTGGGC
PRIMER_RIGHT_1_SEQUENCE=GATAAATGCTCTGCAAGCCC
PRIMER_INTERNAL_1_SEQUENCE=TACACTGCCGTCTTAAGGGA
PRIMER_LEFT_1=143,20
PRIMER_RIGHT_1=230,20
PRIMER_INTERNAL_1=191,20
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.813
PRIMER_INTERNAL_1_TM=58.379
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=0.519124
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.489871
PRIMER_INTERNAL_2_PENALTY=3.030116
PRIMER_LEFT_2_SEQUENCE=TTTTGTAGGTGTTCCTGGGC
PRIMER_RIGHT_2_SEQUENCE=TAAATGCTCTGCAAGCCCTC
PRIMER_INTERNAL_2_SEQUENCE=CCATTTTCTCTAATACACTGCCG
PRIMER_LEFT_2=143,20
PRIMER_RIGHT_2=228,20
PRIMER_INTERNAL_2=178,23
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.490
PRIMER_INTERNAL_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=0.851612
PRIMER_LEFT_3_PENALTY=0.822359
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_INTERNAL_3_PENALTY=2.030276
PRIMER_LEFT_3_SEQUENCE=CCTTCCTGACCTGTTTGCAG
PRIMER_RIGHT_3_SEQUENCE=GCCCAGGAACACCTACAAAA
PRIMER_INTERNAL_3_SEQUENCE=TGTTTTTGAAGGCTCTGTCTCA
PRIMER_LEFT_3=63,20
PRIMER_RIGHT_3=162,20
PRIMER_INTERNAL_3=90,22
PRIMER_LEFT_3_TM=60.822
PRIMER_RIGHT_3_TM=59.971
PRIMER_INTERNAL_3_TM=60.030
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=1.010289
PRIMER_LEFT_4_PENALTY=0.029253
PRIMER_RIGHT_4_PENALTY=0.981036
PRIMER_INTERNAL_4_PENALTY=0.647368
PRIMER_LEFT_4_SEQUENCE=TTTTGTAGGTGTTCCTGGGC
PRIMER_RIGHT_4_SEQUENCE=TGATAAATGCTCTGCAAGCC
PRIMER_INTERNAL_4_SEQUENCE=ACACTGCCGTCTTAAGGGAG
PRIMER_LEFT_4=143,20
PRIMER_RIGHT_4=231,20
PRIMER_INTERNAL_4=192,20
PRIMER_LEFT_4_TM=59.971
PRIMER_RIGHT_4_TM=59.019
PRIMER_INTERNAL_4_TM=59.353
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH830
SEQUENCE_TEMPLATE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGGGAGAAACAAAAGAGTNNAAAGAGAGACCGAACTCAGAACCGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAATACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCAAAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGAAAGATGAAGAGGCAGAATTGAGAGGCTAACATATTTACTCTTGTCTAACTTAAGAGTGCCAGGAAAGCAGATGCTTAGATTTTGTGTCAAAGCTTGTTATTTTTTTCATACTAGGATTATGGTCTTTAG
SEQUENCE_INCLUDED_REGION=31,334
P3_COMMENT=CL is AGAGAGACCGAACTCAGAACC
P3_COMMENT=CR is GACCATAATCCTAGTATGAAAAAAA
P3_COMMENT=CM is AAGTCGTAGCCGATCTCGAG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.388932
PRIMER_LEFT_0_PENALTY=0.010545
PRIMER_RIGHT_0_PENALTY=1.378387
PRIMER_INTERNAL_0_PENALTY=9.873117
PRIMER_LEFT_0_SEQUENCE=AGACCGAAGCAGAAGTCCAA
PRIMER_RIGHT_0_SEQUENCE=GCCTCTCAATTCTGCCTCTT
PRIMER_INTERNAL_0_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
PRIMER_LEFT_0=178,20
PRIMER_RIGHT_0=261,20
PRIMER_INTERNAL_0=199,27
PRIMER_LEFT_0_TM=59.989
PRIMER_RIGHT_0_TM=58.622
PRIMER_INTERNAL_0_TM=57.127
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=25.926
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=1.398023
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=1.387477
PRIMER_INTERNAL_1_PENALTY=9.873117
PRIMER_LEFT_1_SEQUENCE=AGACCGAAGCAGAAGTCCAA
PRIMER_RIGHT_1_SEQUENCE=AGCCTCTCAATTCTGCCTCTT
PRIMER_INTERNAL_1_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
PRIMER_LEFT_1=178,20
PRIMER_RIGHT_1=262,21
PRIMER_INTERNAL_1=199,27
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=59.613
PRIMER_INTERNAL_1_TM=57.127
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=25.926
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=1.451173
PRIMER_LEFT_2_PENALTY=0.010545
PRIMER_RIGHT_2_PENALTY=1.440628
PRIMER_INTERNAL_2_PENALTY=9.873117
PRIMER_LEFT_2_SEQUENCE=AGACCGAAGCAGAAGTCCAA
PRIMER_RIGHT_2_SEQUENCE=CCTCTCAATTCTGCCTCTTCA
PRIMER_INTERNAL_2_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
PRIMER_LEFT_2=178,20
PRIMER_RIGHT_2=260,21
PRIMER_INTERNAL_2=199,27
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=59.559
PRIMER_INTERNAL_2_TM=57.127
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=25.926
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=1.488916
PRIMER_LEFT_3_PENALTY=0.010545
PRIMER_RIGHT_3_PENALTY=1.478371
PRIMER_INTERNAL_3_PENALTY=9.873117
PRIMER_LEFT_3_SEQUENCE=AGACCGAAGCAGAAGTCCAA
PRIMER_RIGHT_3_SEQUENCE=GCCTCTCAATTCTGCCTCTTC
PRIMER_INTERNAL_3_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
PRIMER_LEFT_3=178,20
PRIMER_RIGHT_3=261,21
PRIMER_INTERNAL_3=199,27
PRIMER_LEFT_3_TM=59.989
PRIMER_RIGHT_3_TM=60.478
PRIMER_INTERNAL_3_TM=57.127
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=25.926
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=1.544019
PRIMER_LEFT_4_PENALTY=0.010545
PRIMER_RIGHT_4_PENALTY=1.533474
PRIMER_INTERNAL_4_PENALTY=9.873117
PRIMER_LEFT_4_SEQUENCE=AGACCGAAGCAGAAGTCCAA
PRIMER_RIGHT_4_SEQUENCE=TGTTAGCCTCTCAATTCTGCC
PRIMER_INTERNAL_4_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA
PRIMER_LEFT_4=178,20
PRIMER_RIGHT_4=266,21
PRIMER_INTERNAL_4=199,27
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=59.467
PRIMER_INTERNAL_4_TM=57.127
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_INTERNAL_4_GC_PERCENT=25.926
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH832
SEQUENCE_TEMPLATE=NTTNCNNCAGNTCGACTCTAGAGGATCCCCCTCGTATAAAAGCACCAAGACAGGAATCAAATTGCTCTATTCGGACCCTCATATTAAATAAGAGCAATGAGAGCGAGGGAAAATTGAACTCTCTCAGGTACTGACTGTGGGACCAGACAAGGGATGTAGATTGTCACATTCAATCCTGAAACAAACCTGCCAGGCAAGTCTTCTTCCCATTTTACAAATAAGGAGACAAAAATTAGGAGATTAAATAACTCATCACTGTTTTCAAAATAAGGAGTGTGTGAGGTTTTGTCCCAGGCTCCTGAAGTTTCTGCCTGG
SEQUENCE_INCLUDED_REGION=30,285
P3_COMMENT=CL is AAATTGCTCTATTCGGACCC
P3_COMMENT=CR is GGGACAAAACCTCACACACT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.428832
PRIMER_LEFT_0_PENALTY=0.295091
PRIMER_RIGHT_0_PENALTY=0.133741
PRIMER_INTERNAL_0_PENALTY=2.975617
PRIMER_LEFT_0_SEQUENCE=TGACTGTGGGACCAGACAAG
PRIMER_RIGHT_0_SEQUENCE=GAAGAAGACTTGCCTGGCAG
PRIMER_INTERNAL_0_SEQUENCE=TGTCACATTCAATCCTGAAACA
PRIMER_LEFT_0=131,20
PRIMER_RIGHT_0=205,20
PRIMER_INTERNAL_0=161,22
PRIMER_LEFT_0_TM=59.705
PRIMER_RIGHT_0_TM=60.134
PRIMER_INTERNAL_0_TM=59.024
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.428832
PRIMER_LEFT_1_PENALTY=0.295091
PRIMER_RIGHT_1_PENALTY=0.133741
PRIMER_INTERNAL_1_PENALTY=2.975617
PRIMER_LEFT_1_SEQUENCE=CTGACTGTGGGACCAGACAA
PRIMER_RIGHT_1_SEQUENCE=GAAGAAGACTTGCCTGGCAG
PRIMER_INTERNAL_1_SEQUENCE=TGTCACATTCAATCCTGAAACA
PRIMER_LEFT_1=130,20
PRIMER_RIGHT_1=205,20
PRIMER_INTERNAL_1=161,22
PRIMER_LEFT_1_TM=59.705
PRIMER_RIGHT_1_TM=60.134
PRIMER_INTERNAL_1_TM=59.024
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=0.568163
PRIMER_LEFT_2_PENALTY=0.347318
PRIMER_RIGHT_2_PENALTY=0.220845
PRIMER_INTERNAL_2_PENALTY=0.613219
PRIMER_LEFT_2_SEQUENCE=TGCTCTATTCGGACCCTCAT
PRIMER_RIGHT_2_SEQUENCE=TACATCCCTTGTCTGGTCCC
PRIMER_INTERNAL_2_SEQUENCE=GAGAGCGAGGGAAAATTGAA
PRIMER_LEFT_2=62,20
PRIMER_RIGHT_2=157,20
PRIMER_INTERNAL_2=98,20
PRIMER_LEFT_2_TM=59.653
PRIMER_RIGHT_2_TM=59.779
PRIMER_INTERNAL_2_TM=59.387
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.642409
PRIMER_LEFT_3_PENALTY=0.347318
PRIMER_RIGHT_3_PENALTY=0.295091
PRIMER_INTERNAL_3_PENALTY=0.613219
PRIMER_LEFT_3_SEQUENCE=TGCTCTATTCGGACCCTCAT
PRIMER_RIGHT_3_SEQUENCE=TTGTCTGGTCCCACAGTCAG
PRIMER_INTERNAL_3_SEQUENCE=GAGAGCGAGGGAAAATTGAA
PRIMER_LEFT_3=62,20
PRIMER_RIGHT_3=149,20
PRIMER_INTERNAL_3=98,20
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=59.705
PRIMER_INTERNAL_3_TM=59.387
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=0.642409
PRIMER_LEFT_4_PENALTY=0.347318
PRIMER_RIGHT_4_PENALTY=0.295091
PRIMER_INTERNAL_4_PENALTY=0.613219
PRIMER_LEFT_4_SEQUENCE=TGCTCTATTCGGACCCTCAT
PRIMER_RIGHT_4_SEQUENCE=CTTGTCTGGTCCCACAGTCA
PRIMER_INTERNAL_4_SEQUENCE=GAGAGCGAGGGAAAATTGAA
PRIMER_LEFT_4=62,20
PRIMER_RIGHT_4=150,20
PRIMER_INTERNAL_4=98,20
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=59.705
PRIMER_INTERNAL_4_TM=59.387
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH835
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATGCACTGAGCACTAGACAGTGTGCTAAGTACTGAAGATATACACTAAGATAATCCTCACAACCACCGTATGAATTGATGAGGAAACTAAGCTTTAAAGATGTTGATATTTATAAGGTATAGTGTTTCCTGTTCATGTGTTTGAG
SEQUENCE_INCLUDED_REGION=34,150
P3_COMMENT=CL is CTAATATGCACTGAGCACTAGACA
P3_COMMENT=CR is CACATGAACAGGAAACACTATACC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.968366
PRIMER_LEFT_0_PENALTY=0.040453
PRIMER_RIGHT_0_PENALTY=2.927913
PRIMER_INTERNAL_0_PENALTY=6.882676
PRIMER_LEFT_0_SEQUENCE=TCCTCACAACCACCGTATGA
PRIMER_RIGHT_0_SEQUENCE=TCAAACACATGAACAGGAAACA
PRIMER_INTERNAL_0_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
PRIMER_LEFT_0=92,20
PRIMER_RIGHT_0=182,22
PRIMER_INTERNAL_0=113,26
PRIMER_LEFT_0_TM=59.960
PRIMER_RIGHT_0_TM=59.072
PRIMER_INTERNAL_0_TM=59.117
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=36.364
PRIMER_INTERNAL_0_GC_PERCENT=30.769
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=3.099994
PRIMER_LEFT_1_PENALTY=0.040453
PRIMER_RIGHT_1_PENALTY=3.059541
PRIMER_INTERNAL_1_PENALTY=6.882676
PRIMER_LEFT_1_SEQUENCE=TCCTCACAACCACCGTATGA
PRIMER_RIGHT_1_SEQUENCE=CTCAAACACATGAACAGGAAACA
PRIMER_INTERNAL_1_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
PRIMER_LEFT_1=92,20
PRIMER_RIGHT_1=183,23
PRIMER_INTERNAL_1=113,26
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=60.060
PRIMER_INTERNAL_1_TM=59.117
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_INTERNAL_1_GC_PERCENT=30.769
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=92
PRIMER_PAIR_2_PENALTY=3.112338
PRIMER_LEFT_2_PENALTY=0.040453
PRIMER_RIGHT_2_PENALTY=3.071885
PRIMER_INTERNAL_2_PENALTY=6.882676
PRIMER_LEFT_2_SEQUENCE=TCCTCACAACCACCGTATGA
PRIMER_RIGHT_2_SEQUENCE=TCAAACACATGAACAGGAAACAC
PRIMER_INTERNAL_2_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
PRIMER_LEFT_2=92,20
PRIMER_RIGHT_2=182,23
PRIMER_INTERNAL_2=113,26
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=59.928
PRIMER_INTERNAL_2_TM=59.117
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_INTERNAL_2_GC_PERCENT=30.769
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_3_PENALTY=3.513129
PRIMER_LEFT_3_PENALTY=0.585216
PRIMER_RIGHT_3_PENALTY=2.927913
PRIMER_INTERNAL_3_PENALTY=6.882676
PRIMER_LEFT_3_SEQUENCE=CCTCACAACCACCGTATGAA
PRIMER_RIGHT_3_SEQUENCE=TCAAACACATGAACAGGAAACA
PRIMER_INTERNAL_3_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
PRIMER_LEFT_3=93,20
PRIMER_RIGHT_3=182,22
PRIMER_INTERNAL_3=113,26
PRIMER_LEFT_3_TM=59.415
PRIMER_RIGHT_3_TM=59.072
PRIMER_INTERNAL_3_TM=59.117
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=36.364
PRIMER_INTERNAL_3_GC_PERCENT=30.769
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=3.644757
PRIMER_LEFT_4_PENALTY=0.585216
PRIMER_RIGHT_4_PENALTY=3.059541
PRIMER_INTERNAL_4_PENALTY=6.882676
PRIMER_LEFT_4_SEQUENCE=CCTCACAACCACCGTATGAA
PRIMER_RIGHT_4_SEQUENCE=CTCAAACACATGAACAGGAAACA
PRIMER_INTERNAL_4_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA
PRIMER_LEFT_4=93,20
PRIMER_RIGHT_4=183,23
PRIMER_INTERNAL_4=113,26
PRIMER_LEFT_4_TM=59.415
PRIMER_RIGHT_4_TM=60.060
PRIMER_INTERNAL_4_TM=59.117
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_INTERNAL_4_GC_PERCENT=30.769
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH839
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGGATNTNNTGATGATTGTTGAGATTGAAAAAAAATGGCAGTATCATGAAGAGGTNATAATAAATTCTTATTACAATAGTATTAAAAGTGGTTTTTCACTCAGTCAGGGAGAAAAATTAGATTGTACTTTTTGTCCTCTATGATTTCCTTCAGTTATCTTCTAAATGTTGTTTTTTCCCCACAGCCCCCTTAACATTGTTCTCTACGCACTTCTCAATATACACTTTCATTTGTTTCTCAAGCTTCTTTGTGGATGACTCCTAAATATAG
SEQUENCE_INCLUDED_REGION=34,274
P3_COMMENT=CL is TTGAGATTGAAAAAAAATGGC
P3_COMMENT=CR is TGAAAGTGTATATTGAGAAGTGCG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.037056
PRIMER_LEFT_0_PENALTY=1.133436
PRIMER_RIGHT_0_PENALTY=0.903619
PRIMER_INTERNAL_0_PENALTY=4.837064
PRIMER_LEFT_0_SEQUENCE=TGGTTTTTCACTCAGTCAGGG
PRIMER_RIGHT_0_SEQUENCE=GCTGTGGGGAAAAAACAACA
PRIMER_INTERNAL_0_SEQUENCE=TTTTGTCCTCTATGATTTCCTTCA
PRIMER_LEFT_0=126,21
PRIMER_RIGHT_0=222,20
PRIMER_INTERNAL_0=166,24
PRIMER_LEFT_0_TM=60.133
PRIMER_RIGHT_0_TM=60.904
PRIMER_INTERNAL_0_TM=59.163
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=2.154240
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=2.006945
PRIMER_INTERNAL_1_PENALTY=5.472827
PRIMER_LEFT_1_SEQUENCE=CACAGCCCCCTTAACATTGT
PRIMER_RIGHT_1_SEQUENCE=TCATCCACAAAGAAGCTTGAGA
PRIMER_INTERNAL_1_SEQUENCE=CGCACTTCTCAATATACACTTTCA
PRIMER_LEFT_1=217,20
PRIMER_RIGHT_1=294,22
PRIMER_INTERNAL_1=243,24
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.993
PRIMER_INTERNAL_1_TM=58.527
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=2.390326
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=2.243031
PRIMER_INTERNAL_2_PENALTY=5.472827
PRIMER_LEFT_2_SEQUENCE=CACAGCCCCCTTAACATTGT
PRIMER_RIGHT_2_SEQUENCE=GGAGTCATCCACAAAGAAGCTT
PRIMER_INTERNAL_2_SEQUENCE=CGCACTTCTCAATATACACTTTCA
PRIMER_LEFT_2=217,20
PRIMER_RIGHT_2=298,22
PRIMER_INTERNAL_2=243,24
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=59.757
PRIMER_INTERNAL_2_TM=58.527
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=6.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=2.577766
PRIMER_LEFT_3_PENALTY=0.570821
PRIMER_RIGHT_3_PENALTY=2.006945
PRIMER_INTERNAL_3_PENALTY=5.472827
PRIMER_LEFT_3_SEQUENCE=CAGCCCCCTTAACATTGTTC
PRIMER_RIGHT_3_SEQUENCE=TCATCCACAAAGAAGCTTGAGA
PRIMER_INTERNAL_3_SEQUENCE=CGCACTTCTCAATATACACTTTCA
PRIMER_LEFT_3=219,20
PRIMER_RIGHT_3=294,22
PRIMER_INTERNAL_3=243,24
PRIMER_LEFT_3_TM=59.429
PRIMER_RIGHT_3_TM=59.993
PRIMER_INTERNAL_3_TM=58.527
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=2.653445
PRIMER_LEFT_4_PENALTY=0.147295
PRIMER_RIGHT_4_PENALTY=2.506150
PRIMER_INTERNAL_4_PENALTY=5.472827
PRIMER_LEFT_4_SEQUENCE=CACAGCCCCCTTAACATTGT
PRIMER_RIGHT_4_SEQUENCE=CATCCACAAAGAAGCTTGAGAA
PRIMER_INTERNAL_4_SEQUENCE=CGCACTTCTCAATATACACTTTCA
PRIMER_LEFT_4=217,20
PRIMER_RIGHT_4=293,22
PRIMER_INTERNAL_4=243,24
PRIMER_LEFT_4_TM=59.853
PRIMER_RIGHT_4_TM=59.494
PRIMER_INTERNAL_4_TM=58.527
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=5.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=77
=
SEQUENCE_ID=MH842
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATGCCAAAGAATTAAAAACACACAGAATCATCAAAGCACAATCTGTGTTTGAGATAAATGATAGTCTGAGTCACCTATGTAAGAAGTAACTCTGAAATAGTAGGATAGTATTATCATTTCCTGTAATAGATTCACCTCTCAGCAATTGGTCTGTTTTCATTCTATGGAAACTCTCCGTACTGTAATTTTCATTCTATGGAAACTCCCCATACTGTAATTGGACAGTTTTGGTTTCCACACCAG
SEQUENCE_INCLUDED_REGION=36,246
P3_COMMENT=CL is AAACACACAGAATCATCAAAGC
P3_COMMENT=CR is GTGGAAACCAAAACTGTCCA
P3_COMMENT=CM is TCATTTCCTGTAATAGATTCACCTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.697489
PRIMER_LEFT_0_PENALTY=1.692817
PRIMER_RIGHT_0_PENALTY=1.004672
PRIMER_INTERNAL_0_PENALTY=1.627348
PRIMER_LEFT_0_SEQUENCE=TCAGCAATTGGTCTGTTTTCA
PRIMER_RIGHT_0_SEQUENCE=TGGAAACCAAAACTGTCCAA
PRIMER_INTERNAL_0_SEQUENCE=TCATTCTATGGAAACTCCCCA
PRIMER_LEFT_0=177,21
PRIMER_RIGHT_0=275,20
PRIMER_INTERNAL_0=227,21
PRIMER_LEFT_0_TM=59.307
PRIMER_RIGHT_0_TM=58.995
PRIMER_INTERNAL_0_TM=59.373
PRIMER_LEFT_0_GC_PERCENT=38.095
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=6.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=2.768302
PRIMER_LEFT_1_PENALTY=1.140954
PRIMER_RIGHT_1_PENALTY=1.627348
PRIMER_INTERNAL_1_PENALTY=3.382873
PRIMER_LEFT_1_SEQUENCE=TCACCTCTCAGCAATTGGTCT
PRIMER_RIGHT_1_SEQUENCE=TGGGGAGTTTCCATAGAATGA
PRIMER_INTERNAL_1_SEQUENCE=TTTCATTCTATGGAAACTCTCCG
PRIMER_LEFT_1=170,21
PRIMER_RIGHT_1=247,21
PRIMER_INTERNAL_1=193,23
PRIMER_LEFT_1_TM=59.859
PRIMER_RIGHT_1_TM=59.373
PRIMER_INTERNAL_1_TM=59.617
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=2.825193
PRIMER_LEFT_2_PENALTY=1.197845
PRIMER_RIGHT_2_PENALTY=1.627348
PRIMER_INTERNAL_2_PENALTY=3.382873
PRIMER_LEFT_2_SEQUENCE=TCACCTCTCAGCAATTGGTC
PRIMER_RIGHT_2_SEQUENCE=TGGGGAGTTTCCATAGAATGA
PRIMER_INTERNAL_2_SEQUENCE=TTTCATTCTATGGAAACTCTCCG
PRIMER_LEFT_2=170,20
PRIMER_RIGHT_2=247,21
PRIMER_INTERNAL_2=193,23
PRIMER_LEFT_2_TM=58.802
PRIMER_RIGHT_2_TM=59.373
PRIMER_INTERNAL_2_TM=59.617
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=78
PRIMER_PAIR_3_PENALTY=2.832009
PRIMER_LEFT_3_PENALTY=1.692817
PRIMER_RIGHT_3_PENALTY=1.139192
PRIMER_INTERNAL_3_PENALTY=1.627348
PRIMER_LEFT_3_SEQUENCE=TCAGCAATTGGTCTGTTTTCA
PRIMER_RIGHT_3_SEQUENCE=GTGGAAACCAAAACTGTCCAA
PRIMER_INTERNAL_3_SEQUENCE=TCATTCTATGGAAACTCCCCA
PRIMER_LEFT_3=177,21
PRIMER_RIGHT_3=276,21
PRIMER_INTERNAL_3=227,21
PRIMER_LEFT_3_TM=59.307
PRIMER_RIGHT_3_TM=59.861
PRIMER_INTERNAL_3_TM=59.373
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_INTERNAL_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=6.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=2.874914
PRIMER_LEFT_4_PENALTY=1.247566
PRIMER_RIGHT_4_PENALTY=1.627348
PRIMER_INTERNAL_4_PENALTY=3.382873
PRIMER_LEFT_4_SEQUENCE=TTCACCTCTCAGCAATTGGTC
PRIMER_RIGHT_4_SEQUENCE=TGGGGAGTTTCCATAGAATGA
PRIMER_INTERNAL_4_SEQUENCE=TTTCATTCTATGGAAACTCTCCG
PRIMER_LEFT_4=169,21
PRIMER_RIGHT_4=247,21
PRIMER_INTERNAL_4=193,23
PRIMER_LEFT_4_TM=60.248
PRIMER_RIGHT_4_TM=59.373
PRIMER_INTERNAL_4_TM=59.617
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH851
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTCTGAGAAGGGGAAAATCTGTCTGCTTAAAGATCTCACTAAACAAGTGCTGTACAGTGACAAGTTACTTATTTTCTAATACCTTTCAGAGGCAGATGACGTATTTTATTTTCCTCTGAGGTCAACAAAAGAAATGGGGTATTATCTGATGATCTACTTTAACATTCTCTATATGAGAAACAAGAGTGTCTAATGTAAAACACAGGATAATTGTGTGTGTGTATGTGTATACAAGCATAAAATGTTTCAGGAAATAATTTCTATAAGTTGATTCTGAAAATAAATCAGTAATAAAACAGTTA
SEQUENCE_INCLUDED_REGION=33,304
P3_COMMENT=CL is AAGGGGAAAATCTGTCTGCT
P3_COMMENT=CR is AATTATTTCCTGAAACATTTTATGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.216468
PRIMER_LEFT_0_PENALTY=1.282865
PRIMER_RIGHT_0_PENALTY=0.933604
PRIMER_INTERNAL_0_PENALTY=5.432436
PRIMER_LEFT_0_SEQUENCE=GAAGGGGAAAATCTGTCTGC
PRIMER_RIGHT_0_SEQUENCE=CGTCATCTGCCTCTGAAAGG
PRIMER_INTERNAL_0_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
PRIMER_LEFT_0=41,20
PRIMER_RIGHT_0=135,20
PRIMER_INTERNAL_0=75,24
PRIMER_LEFT_0_TM=58.717
PRIMER_RIGHT_0_TM=60.934
PRIMER_INTERNAL_0_TM=58.568
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=2.229065
PRIMER_LEFT_1_PENALTY=1.295461
PRIMER_RIGHT_1_PENALTY=0.933604
PRIMER_INTERNAL_1_PENALTY=5.432436
PRIMER_LEFT_1_SEQUENCE=GAAGGGGAAAATCTGTCTGCT
PRIMER_RIGHT_1_SEQUENCE=CGTCATCTGCCTCTGAAAGG
PRIMER_INTERNAL_1_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
PRIMER_LEFT_1=41,21
PRIMER_RIGHT_1=135,20
PRIMER_INTERNAL_1=75,24
PRIMER_LEFT_1_TM=59.705
PRIMER_RIGHT_1_TM=60.934
PRIMER_INTERNAL_1_TM=58.568
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=95
PRIMER_PAIR_2_PENALTY=2.229065
PRIMER_LEFT_2_PENALTY=1.295461
PRIMER_RIGHT_2_PENALTY=0.933604
PRIMER_INTERNAL_2_PENALTY=5.432436
PRIMER_LEFT_2_SEQUENCE=AGAAGGGGAAAATCTGTCTGC
PRIMER_RIGHT_2_SEQUENCE=CGTCATCTGCCTCTGAAAGG
PRIMER_INTERNAL_2_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
PRIMER_LEFT_2=40,21
PRIMER_RIGHT_2=135,20
PRIMER_INTERNAL_2=75,24
PRIMER_LEFT_2_TM=59.705
PRIMER_RIGHT_2_TM=60.934
PRIMER_INTERNAL_2_TM=58.568
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=2.706794
PRIMER_LEFT_3_PENALTY=1.773191
PRIMER_RIGHT_3_PENALTY=0.933604
PRIMER_INTERNAL_3_PENALTY=5.432436
PRIMER_LEFT_3_SEQUENCE=AAGGGGAAAATCTGTCTGCTT
PRIMER_RIGHT_3_SEQUENCE=CGTCATCTGCCTCTGAAAGG
PRIMER_INTERNAL_3_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
PRIMER_LEFT_3=42,21
PRIMER_RIGHT_3=135,20
PRIMER_INTERNAL_3=75,24
PRIMER_LEFT_3_TM=59.227
PRIMER_RIGHT_3_TM=60.934
PRIMER_INTERNAL_3_TM=58.568
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=3.080450
PRIMER_LEFT_4_PENALTY=2.146847
PRIMER_RIGHT_4_PENALTY=0.933604
PRIMER_INTERNAL_4_PENALTY=5.432436
PRIMER_LEFT_4_SEQUENCE=AGGGGAAAATCTGTCTGCTT
PRIMER_RIGHT_4_SEQUENCE=CGTCATCTGCCTCTGAAAGG
PRIMER_INTERNAL_4_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG
PRIMER_LEFT_4=43,20
PRIMER_RIGHT_4=135,20
PRIMER_INTERNAL_4=75,24
PRIMER_LEFT_4_TM=57.853
PRIMER_RIGHT_4_TM=60.934
PRIMER_INTERNAL_4_TM=58.568
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=93
=
SEQUENCE_ID=MH861
SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGTGAATTTNCCACAGCCCCAGGGAGAACAATTATAGTGACCAACATTTACCTTTGCTGGAGACTTCCTGAATGGGAGCAGGGGCAGATACAGGGACCTACAGGACTGAGACTGCATAGAAGATGCTGAAGAAGACAGATGATTGCCAAGAGTTAGATCCCCCTCCCTACCATCCCTGGAACTTGGATGCAACCCTGAAGAAGATAGGATAAAAAAATGCTGAAGTCAATGAACTCACCTACATTTAGTAAGATTAGTTTCCTTCTGTCGTTTTTGCCTGAGGAG
SEQUENCE_INCLUDED_REGION=34,289
P3_COMMENT=CL is CAGGGAGAACAATTATAGTGACC
P3_COMMENT=CR is TTGGCAATCATCTGTCTTCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.258372
PRIMER_LEFT_0_PENALTY=0.111077
PRIMER_RIGHT_0_PENALTY=0.147295
PRIMER_INTERNAL_0_PENALTY=2.298898
PRIMER_LEFT_0_SEQUENCE=ACTTGGATGCAACCCTGAAG
PRIMER_RIGHT_0_SEQUENCE=AGGCAAAAACGACAGAAGGA
PRIMER_INTERNAL_0_SEQUENCE=TGCTGAAGTCAATGAACTCACC
PRIMER_LEFT_0=218,20
PRIMER_RIGHT_0=316,20
PRIMER_INTERNAL_0=255,22
PRIMER_LEFT_0_TM=60.111
PRIMER_RIGHT_0_TM=59.853
PRIMER_INTERNAL_0_TM=60.299
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=0.338433
PRIMER_LEFT_1_PENALTY=0.111077
PRIMER_RIGHT_1_PENALTY=0.227356
PRIMER_INTERNAL_1_PENALTY=2.298898
PRIMER_LEFT_1_SEQUENCE=ACTTGGATGCAACCCTGAAG
PRIMER_RIGHT_1_SEQUENCE=GGCAAAAACGACAGAAGGAA
PRIMER_INTERNAL_1_SEQUENCE=TGCTGAAGTCAATGAACTCACC
PRIMER_LEFT_1=218,20
PRIMER_RIGHT_1=315,20
PRIMER_INTERNAL_1=255,22
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=60.227
PRIMER_INTERNAL_1_TM=60.299
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=7.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=98
PRIMER_PAIR_2_PENALTY=0.342653
PRIMER_LEFT_2_PENALTY=0.195358
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_INTERNAL_2_PENALTY=2.298898
PRIMER_LEFT_2_SEQUENCE=GGATGCAACCCTGAAGAAGA
PRIMER_RIGHT_2_SEQUENCE=AGGCAAAAACGACAGAAGGA
PRIMER_INTERNAL_2_SEQUENCE=TGCTGAAGTCAATGAACTCACC
PRIMER_LEFT_2=222,20
PRIMER_RIGHT_2=316,20
PRIMER_INTERNAL_2=255,22
PRIMER_LEFT_2_TM=60.195
PRIMER_RIGHT_2_TM=59.853
PRIMER_INTERNAL_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=7.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=95
PRIMER_PAIR_3_PENALTY=0.422714
PRIMER_LEFT_3_PENALTY=0.195358
PRIMER_RIGHT_3_PENALTY=0.227356
PRIMER_INTERNAL_3_PENALTY=2.298898
PRIMER_LEFT_3_SEQUENCE=GGATGCAACCCTGAAGAAGA
PRIMER_RIGHT_3_SEQUENCE=GGCAAAAACGACAGAAGGAA
PRIMER_INTERNAL_3_SEQUENCE=TGCTGAAGTCAATGAACTCACC
PRIMER_LEFT_3=222,20
PRIMER_RIGHT_3=315,20
PRIMER_INTERNAL_3=255,22
PRIMER_LEFT_3_TM=60.195
PRIMER_RIGHT_3_TM=60.227
PRIMER_INTERNAL_3_TM=60.299
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=0.642468
PRIMER_LEFT_4_PENALTY=0.495173
PRIMER_RIGHT_4_PENALTY=0.147295
PRIMER_INTERNAL_4_PENALTY=2.298898
PRIMER_LEFT_4_SEQUENCE=AACTTGGATGCAACCCTGAA
PRIMER_RIGHT_4_SEQUENCE=AGGCAAAAACGACAGAAGGA
PRIMER_INTERNAL_4_SEQUENCE=TGCTGAAGTCAATGAACTCACC
PRIMER_LEFT_4=217,20
PRIMER_RIGHT_4=316,20
PRIMER_INTERNAL_4=255,22
PRIMER_LEFT_4_TM=60.495
PRIMER_RIGHT_4_TM=59.853
PRIMER_INTERNAL_4_TM=60.299
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=7.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH862
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTATTTGTATTGCTGGAAACAATAGGATAATTTGCTGAGGTTGGGGAGTTTCTCCCNTCCAGAGAGTNCTTAATCTTCCAAAATTTGGTTGAGATCCAAGGTTGATTTTGCTCTACGACTTTTTTTCTGAAGTTTTACTCATTTCCAATAAGAAGGCATGTTTTCCTGCTTCCATGATGATGGAGAACAGGCACATCCTTTCTTGAGTTTCAGCGTGCTTCTGACAGGGAGGGTGAGTGTGAGATTTTTCCTTCTTGTAAGATGGTAGAGAGCCATCATCAGCCTGAGCCATATTTCCAAGTA
SEQUENCE_INCLUDED_REGION=34,302
P3_COMMENT=CL is TATTTGTATTGCTGGAAACAAT
P3_COMMENT=CR is ACTTGGAAATATGGCTCAGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.233904
PRIMER_LEFT_0_PENALTY=0.080962
PRIMER_RIGHT_0_PENALTY=0.152942
PRIMER_INTERNAL_0_PENALTY=0.362999
PRIMER_LEFT_0_SEQUENCE=GCATGTTTTCCTGCTTCCAT
PRIMER_RIGHT_0_SEQUENCE=ACACTCACCCTCCCTGTCAG
PRIMER_INTERNAL_0_SEQUENCE=TGATGGAGAACAGGCACATC
PRIMER_LEFT_0=188,20
PRIMER_RIGHT_0=272,20
PRIMER_INTERNAL_0=210,20
PRIMER_LEFT_0_TM=60.081
PRIMER_RIGHT_0_TM=60.153
PRIMER_INTERNAL_0_TM=59.637
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=0.233904
PRIMER_LEFT_1_PENALTY=0.080962
PRIMER_RIGHT_1_PENALTY=0.152942
PRIMER_INTERNAL_1_PENALTY=0.324185
PRIMER_LEFT_1_SEQUENCE=GCATGTTTTCCTGCTTCCAT
PRIMER_RIGHT_1_SEQUENCE=CTCACACTCACCCTCCCTGT
PRIMER_INTERNAL_1_SEQUENCE=TGAGTTTCAGCGTGCTTCTG
PRIMER_LEFT_1=188,20
PRIMER_RIGHT_1=275,20
PRIMER_INTERNAL_1=236,20
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.153
PRIMER_INTERNAL_1_TM=60.324
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=0.253280
PRIMER_LEFT_2_PENALTY=0.080962
PRIMER_RIGHT_2_PENALTY=0.172318
PRIMER_INTERNAL_2_PENALTY=0.362999
PRIMER_LEFT_2_SEQUENCE=GCATGTTTTCCTGCTTCCAT
PRIMER_RIGHT_2_SEQUENCE=CTCACCCTCCCTGTCAGAAG
PRIMER_INTERNAL_2_SEQUENCE=TGATGGAGAACAGGCACATC
PRIMER_LEFT_2=188,20
PRIMER_RIGHT_2=269,20
PRIMER_INTERNAL_2=210,20
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=59.828
PRIMER_INTERNAL_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=0.314291
PRIMER_LEFT_3_PENALTY=0.161348
PRIMER_RIGHT_3_PENALTY=0.152942
PRIMER_INTERNAL_3_PENALTY=0.324185
PRIMER_LEFT_3_SEQUENCE=TGCTTCCATGATGATGGAGA
PRIMER_RIGHT_3_SEQUENCE=CTCACACTCACCCTCCCTGT
PRIMER_INTERNAL_3_SEQUENCE=TGAGTTTCAGCGTGCTTCTG
PRIMER_LEFT_3=199,20
PRIMER_RIGHT_3=275,20
PRIMER_INTERNAL_3=236,20
PRIMER_LEFT_3_TM=60.161
PRIMER_RIGHT_3_TM=60.153
PRIMER_INTERNAL_3_TM=60.324
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=0.330418
PRIMER_LEFT_4_PENALTY=0.152942
PRIMER_RIGHT_4_PENALTY=0.177475
PRIMER_INTERNAL_4_PENALTY=4.128601
PRIMER_LEFT_4_SEQUENCE=ACAGGGAGGGTGAGTGTGAG
PRIMER_RIGHT_4_SEQUENCE=GGAAATATGGCTCAGGCTGA
PRIMER_INTERNAL_4_SEQUENCE=TGTAAGATGGTAGAGAGCCATCA
PRIMER_LEFT_4=256,20
PRIMER_RIGHT_4=330,20
PRIMER_INTERNAL_4=288,23
PRIMER_LEFT_4_TM=60.153
PRIMER_RIGHT_4_TM=60.177
PRIMER_INTERNAL_4_TM=58.871
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=7.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=4.00
PRIMER_PAIR_4_PRODUCT_SIZE=75
=
SEQUENCE_ID=MH864
SEQUENCE_TEMPLATE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTCAGTGATAAGGACAGGTCTAGAACAAGCGTTCCCAACCCTGGCACCAATGACAGTTTGGACCAAATAACTCTTTGTTTCAGGGGACTGTCCTACACATTGTGGGATGTTTAGCAGCCTCCGTGGCTTCTACCCACTAGATGCCAGCAGCACAACACCCCTCCCCAACAATCATGACAATGAAAATGTCTTTAGACATTGCCAAATATACCTTGTGGGACAAAATGGCCCCTGATTGAGAACCACTGGTTTGGAGTATGGTTATGAGAAGTACAGTCTTGGCATGTGAAGTTCTGGAAATC
SEQUENCE_INCLUDED_REGION=29,302
P3_COMMENT=CL is TTCAGTGATAAGGACAGGTCTA
P3_COMMENT=CR is TTTCCAGAACTTCACATGCC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.400322
PRIMER_LEFT_0_PENALTY=0.151542
PRIMER_RIGHT_0_PENALTY=0.248781
PRIMER_INTERNAL_0_PENALTY=0.056936
PRIMER_LEFT_0_SEQUENCE=CTGGCACCAATGACAGTTTG
PRIMER_RIGHT_0_SEQUENCE=CTAGTGGGTAGAAGCCACGG
PRIMER_INTERNAL_0_SEQUENCE=TTTGTTTCAGGGGACTGTCC
PRIMER_LEFT_0=69,20
PRIMER_RIGHT_0=168,20
PRIMER_INTERNAL_0=102,20
PRIMER_LEFT_0_TM=60.152
PRIMER_RIGHT_0_TM=59.751
PRIMER_INTERNAL_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=7.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.435933
PRIMER_LEFT_1_PENALTY=0.056936
PRIMER_RIGHT_1_PENALTY=0.378997
PRIMER_INTERNAL_1_PENALTY=0.300243
PRIMER_LEFT_1_SEQUENCE=TTTGTTTCAGGGGACTGTCC
PRIMER_RIGHT_1_SEQUENCE=TTGTGCTGCTGGCATCTAGT
PRIMER_INTERNAL_1_SEQUENCE=GTGGGATGTTTAGCAGCCTC
PRIMER_LEFT_1=102,20
PRIMER_RIGHT_1=183,20
PRIMER_INTERNAL_1=130,20
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.621
PRIMER_INTERNAL_1_TM=59.700
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=7.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.435933
PRIMER_LEFT_2_PENALTY=0.056936
PRIMER_RIGHT_2_PENALTY=0.378997
PRIMER_INTERNAL_2_PENALTY=0.300243
PRIMER_LEFT_2_SEQUENCE=TTTGTTTCAGGGGACTGTCC
PRIMER_RIGHT_2_SEQUENCE=GTTGTGCTGCTGGCATCTAG
PRIMER_INTERNAL_2_SEQUENCE=GTGGGATGTTTAGCAGCCTC
PRIMER_LEFT_2=102,20
PRIMER_RIGHT_2=184,20
PRIMER_INTERNAL_2=130,20
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.621
PRIMER_INTERNAL_2_TM=59.700
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=7.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=83
PRIMER_PAIR_3_PENALTY=0.451785
PRIMER_LEFT_3_PENALTY=0.151542
PRIMER_RIGHT_3_PENALTY=0.300243
PRIMER_INTERNAL_3_PENALTY=0.056936
PRIMER_LEFT_3_SEQUENCE=CTGGCACCAATGACAGTTTG
PRIMER_RIGHT_3_SEQUENCE=GAGGCTGCTAAACATCCCAC
PRIMER_INTERNAL_3_SEQUENCE=TTTGTTTCAGGGGACTGTCC
PRIMER_LEFT_3=69,20
PRIMER_RIGHT_3=149,20
PRIMER_INTERNAL_3=102,20
PRIMER_LEFT_3_TM=60.152
PRIMER_RIGHT_3_TM=59.700
PRIMER_INTERNAL_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=81
PRIMER_PAIR_4_PENALTY=0.676879
PRIMER_LEFT_4_PENALTY=0.300243
PRIMER_RIGHT_4_PENALTY=0.376636
PRIMER_INTERNAL_4_PENALTY=0.378997
PRIMER_LEFT_4_SEQUENCE=GTGGGATGTTTAGCAGCCTC
PRIMER_RIGHT_4_SEQUENCE=CATTGTCATGATTGTTGGGG
PRIMER_INTERNAL_4_SEQUENCE=CTAGATGCCAGCAGCACAAC
PRIMER_LEFT_4=130,20
PRIMER_RIGHT_4=210,20
PRIMER_INTERNAL_4=165,20
PRIMER_LEFT_4_TM=59.700
PRIMER_RIGHT_4_TM=59.623
PRIMER_INTERNAL_4_TM=59.621
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH866
SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGATGGCTCCGACGGCTTGTATTCCAGCACAGCAGGGCTTGGCCGTTGTTGCAGACATTTGCCTTCTCTGCNTGGTGGCATGGACTCCATCCAGGACAGGTGTTTGGTTTCGTTTGCTGGGCCGTGATGGTGGAAG
SEQUENCE_INCLUDED_REGION=33,137
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.023931
PRIMER_LEFT_0_PENALTY=0.018700
PRIMER_RIGHT_0_PENALTY=0.005232
PRIMER_INTERNAL_0_PENALTY=0.152999
PRIMER_LEFT_0_SEQUENCE=GCTTGTATTCCAGCACAGCA
PRIMER_RIGHT_0_SEQUENCE=CGAAACCAAACACCTGTCCT
PRIMER_INTERNAL_0_SEQUENCE=TGTTGCAGACATTTGCCTTC
PRIMER_LEFT_0=48,20
PRIMER_RIGHT_0=145,20
PRIMER_INTERNAL_0=80,20
PRIMER_LEFT_0_TM=60.019
PRIMER_RIGHT_0_TM=60.005
PRIMER_INTERNAL_0_TM=59.847
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=0.057144
PRIMER_LEFT_1_PENALTY=0.018700
PRIMER_RIGHT_1_PENALTY=0.038444
PRIMER_INTERNAL_1_PENALTY=0.152999
PRIMER_LEFT_1_SEQUENCE=GCTTGTATTCCAGCACAGCA
PRIMER_RIGHT_1_SEQUENCE=AACACCTGTCCTGGATGGAG
PRIMER_INTERNAL_1_SEQUENCE=TGTTGCAGACATTTGCCTTC
PRIMER_LEFT_1=48,20
PRIMER_RIGHT_1=137,20
PRIMER_INTERNAL_1=80,20
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=59.962
PRIMER_INTERNAL_1_TM=59.847
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=0.141131
PRIMER_LEFT_2_PENALTY=0.135900
PRIMER_RIGHT_2_PENALTY=0.005232
PRIMER_INTERNAL_2_PENALTY=0.152999
PRIMER_LEFT_2_SEQUENCE=TTGTATTCCAGCACAGCAGG
PRIMER_RIGHT_2_SEQUENCE=CGAAACCAAACACCTGTCCT
PRIMER_INTERNAL_2_SEQUENCE=TGTTGCAGACATTTGCCTTC
PRIMER_LEFT_2=50,20
PRIMER_RIGHT_2=145,20
PRIMER_INTERNAL_2=80,20
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.005
PRIMER_INTERNAL_2_TM=59.847
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=96
PRIMER_PAIR_3_PENALTY=0.151269
PRIMER_LEFT_3_PENALTY=0.018700
PRIMER_RIGHT_3_PENALTY=0.132570
PRIMER_INTERNAL_3_PENALTY=0.152999
PRIMER_LEFT_3_SEQUENCE=GCTTGTATTCCAGCACAGCA
PRIMER_RIGHT_3_SEQUENCE=ACGAAACCAAACACCTGTCC
PRIMER_INTERNAL_3_SEQUENCE=TGTTGCAGACATTTGCCTTC
PRIMER_LEFT_3=48,20
PRIMER_RIGHT_3=146,20
PRIMER_INTERNAL_3=80,20
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=59.867
PRIMER_INTERNAL_3_TM=59.847
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=99
PRIMER_PAIR_4_PENALTY=0.157959
PRIMER_LEFT_4_PENALTY=0.018700
PRIMER_RIGHT_4_PENALTY=0.139259
PRIMER_INTERNAL_4_PENALTY=0.152999
PRIMER_LEFT_4_SEQUENCE=GCTTGTATTCCAGCACAGCA
PRIMER_RIGHT_4_SEQUENCE=AAACCAAACACCTGTCCTGG
PRIMER_INTERNAL_4_SEQUENCE=TGTTGCAGACATTTGCCTTC
PRIMER_LEFT_4=48,20
PRIMER_RIGHT_4=143,20
PRIMER_INTERNAL_4=80,20
PRIMER_LEFT_4_TM=60.019
PRIMER_RIGHT_4_TM=59.861
PRIMER_INTERNAL_4_TM=59.847
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH870
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAAGATGCAGGCGGAAGGTTTTTGTGCTGGAGACAATACAGGGGATGACTTCTTAAATCCGTCGTCGTCATCCTCCATTAGTGATAAATAGTCACCTTGGCTCAGATTCGTCAGACAAATACTATATTGCTACTTGTTATCTTCTAGTAACCTTCAGAAAAACTTTAGTGTTTACTTAAAAAATGAGGAAAAAAAAGAATCATTATGTAGAAAAAAATGAGAACATTTTGCTTCGTTTCAAAAATTATTTATTCAAATTAGTGAACAAAACATGGTCTATCGGAATAGGCTCATTAAACAG
SEQUENCE_INCLUDED_REGION=34,303
P3_COMMENT=CL is GAAAGATGCAGGCGGAAG
P3_COMMENT=CR is TGTTTAATGAGCCTATTCCG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.629967
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=0.557015
PRIMER_INTERNAL_0_PENALTY=0.727969
PRIMER_LEFT_0_SEQUENCE=GCTGGAGACAATACAGGGGA
PRIMER_RIGHT_0_SEQUENCE=TGTCTGACGAATCTGAGCCA
PRIMER_INTERNAL_0_SEQUENCE=TCTTAAATCCGTCGTCGTCA
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=152,20
PRIMER_INTERNAL_0=86,20
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=60.557
PRIMER_INTERNAL_0_TM=59.272
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=0.874143
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.801192
PRIMER_INTERNAL_1_PENALTY=0.727969
PRIMER_LEFT_1_SEQUENCE=GCTGGAGACAATACAGGGGA
PRIMER_RIGHT_1_SEQUENCE=CGAATCTGAGCCAAGGTGAC
PRIMER_INTERNAL_1_SEQUENCE=TCTTAAATCCGTCGTCGTCA
PRIMER_LEFT_1=61,20
PRIMER_RIGHT_1=145,20
PRIMER_INTERNAL_1=86,20
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=60.801
PRIMER_INTERNAL_1_TM=59.272
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=0.874143
PRIMER_LEFT_2_PENALTY=0.072951
PRIMER_RIGHT_2_PENALTY=0.801192
PRIMER_INTERNAL_2_PENALTY=0.727969
PRIMER_LEFT_2_SEQUENCE=GCTGGAGACAATACAGGGGA
PRIMER_RIGHT_2_SEQUENCE=ACGAATCTGAGCCAAGGTGA
PRIMER_INTERNAL_2_SEQUENCE=TCTTAAATCCGTCGTCGTCA
PRIMER_LEFT_2=61,20
PRIMER_RIGHT_2=146,20
PRIMER_INTERNAL_2=86,20
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=60.801
PRIMER_INTERNAL_2_TM=59.272
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=0.874143
PRIMER_LEFT_3_PENALTY=0.072951
PRIMER_RIGHT_3_PENALTY=0.801192
PRIMER_INTERNAL_3_PENALTY=0.727969
PRIMER_LEFT_3_SEQUENCE=GCTGGAGACAATACAGGGGA
PRIMER_RIGHT_3_SEQUENCE=GACGAATCTGAGCCAAGGTG
PRIMER_INTERNAL_3_SEQUENCE=TCTTAAATCCGTCGTCGTCA
PRIMER_LEFT_3=61,20
PRIMER_RIGHT_3=147,20
PRIMER_INTERNAL_3=86,20
PRIMER_LEFT_3_TM=60.073
PRIMER_RIGHT_3_TM=60.801
PRIMER_INTERNAL_3_TM=59.272
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=87
PRIMER_PAIR_4_PENALTY=0.874143
PRIMER_LEFT_4_PENALTY=0.072951
PRIMER_RIGHT_4_PENALTY=0.801192
PRIMER_INTERNAL_4_PENALTY=0.727969
PRIMER_LEFT_4_SEQUENCE=GCTGGAGACAATACAGGGGA
PRIMER_RIGHT_4_SEQUENCE=TGACGAATCTGAGCCAAGGT
PRIMER_INTERNAL_4_SEQUENCE=TCTTAAATCCGTCGTCGTCA
PRIMER_LEFT_4=61,20
PRIMER_RIGHT_4=148,20
PRIMER_INTERNAL_4=86,20
PRIMER_LEFT_4_TM=60.073
PRIMER_RIGHT_4_TM=60.801
PRIMER_INTERNAL_4_TM=59.272
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH877
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGATCAGTCCTCATCTTATTTTATATCCTCAGCTTCTAACATGTTGTCTGGTGCATAGTTGGCACTAAATAAATATTTTTGAATGAATTAAGTAATGTCAGCAGATGACTAGATATTATATGCTTCTTGAATTCTTTCCTAATCTCTTCAACCACCACACCTATCTCCCACCCTACTGAGAGTTGAGTATTTCCTCTTCTGTATGTCAATGAAAATTTGCAAGTAACTTAGGTGACCAATCATATACCCAGGTTTACCTGAGACATCCCTAGTTTATGCCTATTGTTCTTGCATAACTATT
SEQUENCE_INCLUDED_REGION=33,308
P3_COMMENT=CL is CTCCTTGAGATCAGTCCTCA
P3_COMMENT=CR is AGTTATGCAAGAACAATAGGCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.557213
PRIMER_LEFT_0_PENALTY=0.237616
PRIMER_RIGHT_0_PENALTY=2.319597
PRIMER_INTERNAL_0_PENALTY=4.462902
PRIMER_LEFT_0_SEQUENCE=CACCACACCTATCTCCCACC
PRIMER_RIGHT_0_SEQUENCE=CCTGGGTATATGATTGGTCACC
PRIMER_INTERNAL_0_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
PRIMER_LEFT_0=192,20
PRIMER_RIGHT_0=291,22
PRIMER_INTERNAL_0=237,24
PRIMER_LEFT_0_TM=60.238
PRIMER_RIGHT_0_TM=60.320
PRIMER_INTERNAL_0_TM=59.537
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=3.351604
PRIMER_LEFT_1_PENALTY=1.032007
PRIMER_RIGHT_1_PENALTY=2.319597
PRIMER_INTERNAL_1_PENALTY=4.462902
PRIMER_LEFT_1_SEQUENCE=ACCACACCTATCTCCCACCC
PRIMER_RIGHT_1_SEQUENCE=CCTGGGTATATGATTGGTCACC
PRIMER_INTERNAL_1_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
PRIMER_LEFT_1=193,20
PRIMER_RIGHT_1=291,22
PRIMER_INTERNAL_1=237,24
PRIMER_LEFT_1_TM=61.032
PRIMER_RIGHT_1_TM=60.320
PRIMER_INTERNAL_1_TM=59.537
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=29.167
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=3.479771
PRIMER_LEFT_2_PENALTY=1.160174
PRIMER_RIGHT_2_PENALTY=2.319597
PRIMER_INTERNAL_2_PENALTY=4.462902
PRIMER_LEFT_2_SEQUENCE=CCACACCTATCTCCCACCCT
PRIMER_RIGHT_2_SEQUENCE=CCTGGGTATATGATTGGTCACC
PRIMER_INTERNAL_2_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
PRIMER_LEFT_2=194,20
PRIMER_RIGHT_2=291,22
PRIMER_INTERNAL_2=237,24
PRIMER_LEFT_2_TM=61.160
PRIMER_RIGHT_2_TM=60.320
PRIMER_INTERNAL_2_TM=59.537
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=29.167
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=8.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=98
PRIMER_PAIR_3_PENALTY=3.502965
PRIMER_LEFT_3_PENALTY=1.183368
PRIMER_RIGHT_3_PENALTY=2.319597
PRIMER_INTERNAL_3_PENALTY=4.462902
PRIMER_LEFT_3_SEQUENCE=CCACACCTATCTCCCACCC
PRIMER_RIGHT_3_SEQUENCE=CCTGGGTATATGATTGGTCACC
PRIMER_INTERNAL_3_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
PRIMER_LEFT_3=194,19
PRIMER_RIGHT_3=291,22
PRIMER_INTERNAL_3=237,24
PRIMER_LEFT_3_TM=60.183
PRIMER_RIGHT_3_TM=60.320
PRIMER_INTERNAL_3_TM=59.537
PRIMER_LEFT_3_GC_PERCENT=63.158
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=29.167
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=3.613467
PRIMER_LEFT_4_PENALTY=1.293870
PRIMER_RIGHT_4_PENALTY=2.319597
PRIMER_INTERNAL_4_PENALTY=4.462902
PRIMER_LEFT_4_SEQUENCE=TCCCACCCTACTGAGAGTTGA
PRIMER_RIGHT_4_SEQUENCE=CCTGGGTATATGATTGGTCACC
PRIMER_INTERNAL_4_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA
PRIMER_LEFT_4=205,21
PRIMER_RIGHT_4=291,22
PRIMER_INTERNAL_4=237,24
PRIMER_LEFT_4_TM=59.706
PRIMER_RIGHT_4_TM=60.320
PRIMER_INTERNAL_4_TM=59.537
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=29.167
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH880
SEQUENCE_TEMPLATE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTTTCGTTTTGGGGTGGAAAACTTGTATCTGAAATGTTCCTGTGAATGTTAAGAAAGTTAAAAATGAATTATTTTTCTCTTCTTTAATGCCCATCCATTTTTTGTCTTCATCTGAGAATGACTTTCCTAAAGACAACATTCGTATACAGAAGCCAGACCTATGCAAGAGAAAGTATAATAATTTTCAATGAGGCAAAGGTCATTAATATGACCTACATCTTCATAAAATGCGAATTTAATCACGAGTCATGACGATTGTTCTTTGAGAGGCTTTTTATTCCCCATGGTGAAAAAAGATTTGTAGT
SEQUENCE_INCLUDED_REGION=29,305
P3_COMMENT=CL is TCGTTTTGGGGTGGAAAACT
P3_COMMENT=CR is TACAAATCTTTTTTCACCATGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.064566
PRIMER_LEFT_0_PENALTY=0.692222
PRIMER_RIGHT_0_PENALTY=0.372343
PRIMER_INTERNAL_0_PENALTY=4.384367
PRIMER_LEFT_0_SEQUENCE=TTTTGGGGTGGAAAACTTGT
PRIMER_RIGHT_0_SEQUENCE=GACAAAAAATGGATGGGCAT
PRIMER_INTERNAL_0_SEQUENCE=TCTGAAATGTTCCTGTGAATGTT
PRIMER_LEFT_0=35,20
PRIMER_RIGHT_0=134,20
PRIMER_INTERNAL_0=56,23
PRIMER_LEFT_0_TM=59.308
PRIMER_RIGHT_0_TM=59.628
PRIMER_INTERNAL_0_TM=58.616
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_INTERNAL_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=1.284986
PRIMER_LEFT_1_PENALTY=0.309369
PRIMER_RIGHT_1_PENALTY=0.975617
PRIMER_INTERNAL_1_PENALTY=4.297164
PRIMER_LEFT_1_SEQUENCE=TGCCCATCCATTTTTTGTCT
PRIMER_RIGHT_1_SEQUENCE=TTGCATAGGTCTGGCTTCTG
PRIMER_INTERNAL_1_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
PRIMER_LEFT_1=116,20
PRIMER_RIGHT_1=194,20
PRIMER_INTERNAL_1=147,24
PRIMER_LEFT_1_TM=60.309
PRIMER_RIGHT_1_TM=59.024
PRIMER_INTERNAL_1_TM=59.703
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=79
PRIMER_PAIR_2_PENALTY=1.332157
PRIMER_LEFT_2_PENALTY=0.309369
PRIMER_RIGHT_2_PENALTY=1.022788
PRIMER_INTERNAL_2_PENALTY=4.297164
PRIMER_LEFT_2_SEQUENCE=TGCCCATCCATTTTTTGTCT
PRIMER_RIGHT_2_SEQUENCE=CTTGCATAGGTCTGGCTTCTG
PRIMER_INTERNAL_2_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
PRIMER_LEFT_2=116,20
PRIMER_RIGHT_2=195,21
PRIMER_INTERNAL_2=147,24
PRIMER_LEFT_2_TM=60.309
PRIMER_RIGHT_2_TM=60.023
PRIMER_INTERNAL_2_TM=59.703
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=1.347960
PRIMER_LEFT_3_PENALTY=0.372343
PRIMER_RIGHT_3_PENALTY=0.975617
PRIMER_INTERNAL_3_PENALTY=4.297164
PRIMER_LEFT_3_SEQUENCE=ATGCCCATCCATTTTTTGTC
PRIMER_RIGHT_3_SEQUENCE=TTGCATAGGTCTGGCTTCTG
PRIMER_INTERNAL_3_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
PRIMER_LEFT_3=115,20
PRIMER_RIGHT_3=194,20
PRIMER_INTERNAL_3=147,24
PRIMER_LEFT_3_TM=59.628
PRIMER_RIGHT_3_TM=59.024
PRIMER_INTERNAL_3_TM=59.703
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=1.395132
PRIMER_LEFT_4_PENALTY=0.372343
PRIMER_RIGHT_4_PENALTY=1.022788
PRIMER_INTERNAL_4_PENALTY=4.297164
PRIMER_LEFT_4_SEQUENCE=ATGCCCATCCATTTTTTGTC
PRIMER_RIGHT_4_SEQUENCE=CTTGCATAGGTCTGGCTTCTG
PRIMER_INTERNAL_4_SEQUENCE=TGACTTTCCTAAAGACAACATTCG
PRIMER_LEFT_4=115,20
PRIMER_RIGHT_4=195,21
PRIMER_INTERNAL_4=147,24
PRIMER_LEFT_4_TM=59.628
PRIMER_RIGHT_4_TM=60.023
PRIMER_INTERNAL_4_TM=59.703
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=81
=
SEQUENCE_ID=MH881
SEQUENCE_TEMPLATE=ATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCAGTTTCAACTGGAGAGATCTAGATAGCATTAAGTGTTTATTTAGGTGTGGCAGACTGAATCCTCTTGACCTGTGAACATATACTATTTGACTAGAGTNATGTTTTGCCTTTTTAAATTAAATTTGAGTAGTTTTGGTTGGAGCATTACTACTATTTGCAACAATCCTCAAAACTCCCTATTTATTGCTTTTTGCTTGGCTCACTTCACATCCATTGTAGGCATTTGTCTGTGTTCTGGTTTAATGCTGTGAGTTTTAATAAGAGTTTCTGTACGGAGCCTGAGATATCTCTATAAGAAGAAAAA
SEQUENCE_INCLUDED_REGION=30,272
P3_COMMENT=CL is TTTCAACTGGAGAGATCTAGATAGC
P3_COMMENT=CR is GAAGTGAGCCAAGCAAAAAG
P3_COMMENT=CM is TTAAGTGTTTATTTAGGTGTGGCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.964268
PRIMER_LEFT_0_PENALTY=1.103105
PRIMER_RIGHT_0_PENALTY=0.861164
PRIMER_INTERNAL_0_PENALTY=0.008461
PRIMER_LEFT_0_SEQUENCE=TGCAACAATCCTCAAAACTCC
PRIMER_RIGHT_0_SEQUENCE=CCAGAACACAGACAAATGCC
PRIMER_INTERNAL_0_SEQUENCE=TGCTTGGCTCACTTCACATC
PRIMER_LEFT_0=188,21
PRIMER_RIGHT_0=270,20
PRIMER_INTERNAL_0=224,20
PRIMER_LEFT_0_TM=60.103
PRIMER_RIGHT_0_TM=59.139
PRIMER_INTERNAL_0_TM=59.992
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=83
PRIMER_PAIR_1_PENALTY=2.130697
PRIMER_LEFT_1_PENALTY=1.103105
PRIMER_RIGHT_1_PENALTY=1.027592
PRIMER_INTERNAL_1_PENALTY=0.008461
PRIMER_LEFT_1_SEQUENCE=TGCAACAATCCTCAAAACTCC
PRIMER_RIGHT_1_SEQUENCE=ACCAGAACACAGACAAATGCC
PRIMER_INTERNAL_1_SEQUENCE=TGCTTGGCTCACTTCACATC
PRIMER_LEFT_1=188,21
PRIMER_RIGHT_1=271,21
PRIMER_INTERNAL_1=224,20
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=60.028
PRIMER_INTERNAL_1_TM=59.992
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=84
PRIMER_PAIR_2_PENALTY=2.223677
PRIMER_LEFT_2_PENALTY=2.215215
PRIMER_RIGHT_2_PENALTY=0.008461
PRIMER_INTERNAL_2_PENALTY=1.103105
PRIMER_LEFT_2_SEQUENCE=TTGAGTAGTTTTGGTTGGAGCA
PRIMER_RIGHT_2_SEQUENCE=GATGTGAAGTGAGCCAAGCA
PRIMER_INTERNAL_2_SEQUENCE=TGCAACAATCCTCAAAACTCC
PRIMER_LEFT_2=155,22
PRIMER_RIGHT_2=243,20
PRIMER_INTERNAL_2=188,21
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=59.992
PRIMER_INTERNAL_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=2.266708
PRIMER_LEFT_3_PENALTY=1.103105
PRIMER_RIGHT_3_PENALTY=1.163603
PRIMER_INTERNAL_3_PENALTY=0.008461
PRIMER_LEFT_3_SEQUENCE=TGCAACAATCCTCAAAACTCC
PRIMER_RIGHT_3_SEQUENCE=CCAGAACACAGACAAATGCCT
PRIMER_INTERNAL_3_SEQUENCE=TGCTTGGCTCACTTCACATC
PRIMER_LEFT_3=188,21
PRIMER_RIGHT_3=270,21
PRIMER_INTERNAL_3=224,20
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=60.164
PRIMER_INTERNAL_3_TM=59.992
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=2.384687
PRIMER_LEFT_4_PENALTY=2.215215
PRIMER_RIGHT_4_PENALTY=0.169471
PRIMER_INTERNAL_4_PENALTY=1.103105
PRIMER_LEFT_4_SEQUENCE=TTGAGTAGTTTTGGTTGGAGCA
PRIMER_RIGHT_4_SEQUENCE=TGGATGTGAAGTGAGCCAAG
PRIMER_INTERNAL_4_SEQUENCE=TGCAACAATCCTCAAAACTCC
PRIMER_LEFT_4=155,22
PRIMER_RIGHT_4=245,20
PRIMER_INTERNAL_4=188,21
PRIMER_LEFT_4_TM=59.785
PRIMER_RIGHT_4_TM=59.831
PRIMER_INTERNAL_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH889
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCTCCTGTTTGCTCATTTTGAAGAGACTCATTACGGACAATGGTCCTCAATGTTAATGAAGTGACACAATCCAATTAGAAGATTGCAGTTACACATGGTAGTGGTAGAATGAGAGGANTTGCTACCTAACAGGTAATGTCACTGTTTGTGCCTTGTTTTTGCCCTCTGTGGGGTTGTTTCTGCTTTGGTCCAGATCTCACTCTGTCCTTGGGCCTACTTTGGGATTCTGCCATCTTCTGAATGCTGGTAAATGCCTAAG
SEQUENCE_INCLUDED_REGION=36,262
P3_COMMENT=CL is CTCATTACGGACAATGGTCC
P3_COMMENT=CR is AGGCATTTACCAGCATTCAG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.261146
PRIMER_LEFT_0_PENALTY=0.247539
PRIMER_RIGHT_0_PENALTY=0.013607
PRIMER_INTERNAL_0_PENALTY=0.148509
PRIMER_LEFT_0_SEQUENCE=TGTTTGTGCCTTGTTTTTGC
PRIMER_RIGHT_0_SEQUENCE=GAAGATGGCAGAATCCCAAA
PRIMER_INTERNAL_0_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
PRIMER_LEFT_0=181,20
PRIMER_RIGHT_0=275,20
PRIMER_INTERNAL_0=204,20
PRIMER_LEFT_0_TM=59.752
PRIMER_RIGHT_0_TM=60.014
PRIMER_INTERNAL_0_TM=60.149
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=95
PRIMER_PAIR_1_PENALTY=0.306236
PRIMER_LEFT_1_PENALTY=0.148509
PRIMER_RIGHT_1_PENALTY=0.157727
PRIMER_INTERNAL_1_PENALTY=0.278847
PRIMER_LEFT_1_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
PRIMER_RIGHT_1_SEQUENCE=TCAGAAGATGGCAGAATCCC
PRIMER_INTERNAL_1_SEQUENCE=CACTCTGTCCTTGGGCCTAC
PRIMER_LEFT_1=204,20
PRIMER_RIGHT_1=278,20
PRIMER_INTERNAL_1=236,20
PRIMER_LEFT_1_TM=60.149
PRIMER_RIGHT_1_TM=60.158
PRIMER_INTERNAL_1_TM=59.721
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=75
PRIMER_PAIR_2_PENALTY=0.313862
PRIMER_LEFT_2_PENALTY=0.247539
PRIMER_RIGHT_2_PENALTY=0.066323
PRIMER_INTERNAL_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=TGTTTGTGCCTTGTTTTTGC
PRIMER_RIGHT_2_SEQUENCE=TGGCAGAATCCCAAAGTAGG
PRIMER_INTERNAL_2_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
PRIMER_LEFT_2=181,20
PRIMER_RIGHT_2=270,20
PRIMER_INTERNAL_2=204,20
PRIMER_LEFT_2_TM=59.752
PRIMER_RIGHT_2_TM=60.066
PRIMER_INTERNAL_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=90
PRIMER_PAIR_3_PENALTY=0.374652
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.226143
PRIMER_INTERNAL_3_PENALTY=0.271680
PRIMER_LEFT_3_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
PRIMER_RIGHT_3_SEQUENCE=ACCAGCATTCAGAAGATGGC
PRIMER_INTERNAL_3_SEQUENCE=CTGTCCTTGGGCCTACTTTG
PRIMER_LEFT_3=204,20
PRIMER_RIGHT_3=286,20
PRIMER_INTERNAL_3=240,20
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=60.226
PRIMER_INTERNAL_3_TM=59.728
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=5.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=83
PRIMER_PAIR_4_PENALTY=0.405266
PRIMER_LEFT_4_PENALTY=0.247539
PRIMER_RIGHT_4_PENALTY=0.157727
PRIMER_INTERNAL_4_PENALTY=0.148509
PRIMER_LEFT_4_SEQUENCE=TGTTTGTGCCTTGTTTTTGC
PRIMER_RIGHT_4_SEQUENCE=TCAGAAGATGGCAGAATCCC
PRIMER_INTERNAL_4_SEQUENCE=CTGTGGGGTTGTTTCTGCTT
PRIMER_LEFT_4=181,20
PRIMER_RIGHT_4=278,20
PRIMER_INTERNAL_4=204,20
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=60.158
PRIMER_INTERNAL_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH901
SEQUENCE_TEMPLATE=AGGGAAACTTCTCTGTGAGANNAGGATCAGGGCATGGCTGGGTGGGAAAGCCAGGAGGGTTTCCTGAAGGAGACATCACCCTAGTCTAATAATTAGCTTTAATTATCCTCACCACAGCCCTAGAAGGAAGGCACTGNTTATCTCTCATTTTACTTTTCTCTGAAGTTAAGAGAAATTAAGTGACTTGCCCAAGGTGATGGAG
SEQUENCE_INCLUDED_REGION=0,202
P3_COMMENT=CL is GGGTTTCCTGAAGGAGACAT
P3_COMMENT=CR is ACCTTGGGCAAGTCACTTAA
P3_COMMENT=CM is ATTAGCTTTAATTATCCTCACCACA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.468578
PRIMER_LEFT_0_PENALTY=0.329319
PRIMER_RIGHT_0_PENALTY=0.139259
PRIMER_INTERNAL_0_PENALTY=9.921269
PRIMER_LEFT_0_SEQUENCE=AGGAGGGTTTCCTGAAGGAG
PRIMER_RIGHT_0_SEQUENCE=CCTTCTAGGGCTGTGGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CATCACCCTAGTCTAATAATTAGCTTT
PRIMER_LEFT_0=52,20
PRIMER_RIGHT_0=126,20
PRIMER_INTERNAL_0=73,27
PRIMER_LEFT_0_TM=59.671
PRIMER_RIGHT_0_TM=59.861
PRIMER_INTERNAL_0_TM=57.079
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=9.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.560031
PRIMER_LEFT_1_PENALTY=0.139259
PRIMER_RIGHT_1_PENALTY=0.420772
PRIMER_INTERNAL_1_PENALTY=9.339364
PRIMER_LEFT_1_SEQUENCE=CTCACCACAGCCCTAGAAGG
PRIMER_RIGHT_1_SEQUENCE=ATCACCTTGGGCAAGTCACT
PRIMER_INTERNAL_1_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT
PRIMER_LEFT_1=107,20
PRIMER_RIGHT_1=197,20
PRIMER_INTERNAL_1=140,27
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=59.579
PRIMER_INTERNAL_1_TM=57.661
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=29.630
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=91
PRIMER_PAIR_2_PENALTY=0.560031
PRIMER_LEFT_2_PENALTY=0.139259
PRIMER_RIGHT_2_PENALTY=0.420772
PRIMER_INTERNAL_2_PENALTY=9.339364
PRIMER_LEFT_2_SEQUENCE=CCTCACCACAGCCCTAGAAG
PRIMER_RIGHT_2_SEQUENCE=ATCACCTTGGGCAAGTCACT
PRIMER_INTERNAL_2_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT
PRIMER_LEFT_2=106,20
PRIMER_RIGHT_2=197,20
PRIMER_INTERNAL_2=140,27
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=59.579
PRIMER_INTERNAL_2_TM=57.661
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=29.630
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=92
PRIMER_PAIR_3_PENALTY=0.575757
PRIMER_LEFT_3_PENALTY=0.329319
PRIMER_RIGHT_3_PENALTY=0.246438
PRIMER_INTERNAL_3_PENALTY=9.921269
PRIMER_LEFT_3_SEQUENCE=AGGAGGGTTTCCTGAAGGAG
PRIMER_RIGHT_3_SEQUENCE=TTCCTTCTAGGGCTGTGGTG
PRIMER_INTERNAL_3_SEQUENCE=CATCACCCTAGTCTAATAATTAGCTTT
PRIMER_LEFT_3=52,20
PRIMER_RIGHT_3=128,20
PRIMER_INTERNAL_3=73,27
PRIMER_LEFT_3_TM=59.671
PRIMER_RIGHT_3_TM=60.246
PRIMER_INTERNAL_3_TM=57.079
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=33.333
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=9.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=77
PRIMER_PAIR_4_PENALTY=0.667209
PRIMER_LEFT_4_PENALTY=0.246438
PRIMER_RIGHT_4_PENALTY=0.420772
PRIMER_INTERNAL_4_PENALTY=9.339364
PRIMER_LEFT_4_SEQUENCE=CACCACAGCCCTAGAAGGAA
PRIMER_RIGHT_4_SEQUENCE=ATCACCTTGGGCAAGTCACT
PRIMER_INTERNAL_4_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT
PRIMER_LEFT_4=109,20
PRIMER_RIGHT_4=197,20
PRIMER_INTERNAL_4=140,27
PRIMER_LEFT_4_TM=60.246
PRIMER_RIGHT_4_TM=59.579
PRIMER_INTERNAL_4_TM=57.661
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=29.630
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=5.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH903
SEQUENCE_TEMPLATE=CATGGTGTACTACCTGGGTNTTGATAATGGTTATTCAGGGACCAAGGGCACTTTAGTCAGAAGGTGATTGGTCCTGCCAGANCTGAGTCCATCGCTTCATGGCAGTGGGTTCCCTTGTGGCCCAGGGTGTGTCTAGAAATGTTGTCCAGGAG
SEQUENCE_INCLUDED_REGION=0,152
P3_COMMENT=CL is TTGATAATGGTTATTCAGGGA
P3_COMMENT=CR is CCTGGACAACATTTCTAGACA
P3_COMMENT=CM is CAAGGGCACTTTAGTCAGAAGG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.767470
PRIMER_LEFT_0_PENALTY=0.656393
PRIMER_RIGHT_0_PENALTY=0.111077
PRIMER_INTERNAL_0_PENALTY=1.772556
PRIMER_LEFT_0_SEQUENCE=CAGAAGGTGATTGGTCCTGC
PRIMER_RIGHT_0_SEQUENCE=TTTCTAGACACACCCTGGGC
PRIMER_INTERNAL_0_SEQUENCE=CTGAGTCCATCGCTTCATGG
PRIMER_LEFT_0=57,20
PRIMER_RIGHT_0=138,20
PRIMER_INTERNAL_0=82,20
PRIMER_LEFT_0_TM=60.656
PRIMER_RIGHT_0_TM=60.111
PRIMER_INTERNAL_0_TM=61.773
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=82
PRIMER_PAIR_1_PENALTY=1.470028
PRIMER_LEFT_1_PENALTY=1.358951
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_INTERNAL_1_PENALTY=1.772556
PRIMER_LEFT_1_SEQUENCE=TCAGAAGGTGATTGGTCCTG
PRIMER_RIGHT_1_SEQUENCE=TTTCTAGACACACCCTGGGC
PRIMER_INTERNAL_1_SEQUENCE=CTGAGTCCATCGCTTCATGG
PRIMER_LEFT_1=56,20
PRIMER_RIGHT_1=138,20
PRIMER_INTERNAL_1=82,20
PRIMER_LEFT_1_TM=58.641
PRIMER_RIGHT_1_TM=60.111
PRIMER_INTERNAL_1_TM=61.773
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=1.540483
PRIMER_LEFT_2_PENALTY=1.429406
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_INTERNAL_2_PENALTY=1.772556
PRIMER_LEFT_2_SEQUENCE=AGAAGGTGATTGGTCCTGCC
PRIMER_RIGHT_2_SEQUENCE=TTTCTAGACACACCCTGGGC
PRIMER_INTERNAL_2_SEQUENCE=CTGAGTCCATCGCTTCATGG
PRIMER_LEFT_2=58,20
PRIMER_RIGHT_2=138,20
PRIMER_INTERNAL_2=82,20
PRIMER_LEFT_2_TM=61.429
PRIMER_RIGHT_2_TM=60.111
PRIMER_INTERNAL_2_TM=61.773
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=1.546895
PRIMER_LEFT_3_PENALTY=1.435818
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_INTERNAL_3_PENALTY=1.772556
PRIMER_LEFT_3_SEQUENCE=GTCAGAAGGTGATTGGTCCTG
PRIMER_RIGHT_3_SEQUENCE=TTTCTAGACACACCCTGGGC
PRIMER_INTERNAL_3_SEQUENCE=CTGAGTCCATCGCTTCATGG
PRIMER_LEFT_3=55,21
PRIMER_RIGHT_3=138,20
PRIMER_INTERNAL_3=82,20
PRIMER_LEFT_3_TM=59.564
PRIMER_RIGHT_3_TM=60.111
PRIMER_INTERNAL_3_TM=61.773
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=84
PRIMER_PAIR_4_PENALTY=1.579343
PRIMER_LEFT_4_PENALTY=1.468266
PRIMER_RIGHT_4_PENALTY=0.111077
PRIMER_INTERNAL_4_PENALTY=1.772556
PRIMER_LEFT_4_SEQUENCE=GAAGGTGATTGGTCCTGCC
PRIMER_RIGHT_4_SEQUENCE=TTTCTAGACACACCCTGGGC
PRIMER_INTERNAL_4_SEQUENCE=CTGAGTCCATCGCTTCATGG
PRIMER_LEFT_4=59,19
PRIMER_RIGHT_4=138,20
PRIMER_INTERNAL_4=82,20
PRIMER_LEFT_4_TM=60.468
PRIMER_RIGHT_4_TM=60.111
PRIMER_INTERNAL_4_TM=61.773
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=80
=
SEQUENCE_ID=MH904
SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGATTTTACTGCTTTTNCTTTCTGTTTAACTTTCCTTTCCTTTTCTCCCTTGGTTAGAGTGTGGGACACTAGATCAGCAAAATTAACAAGATCATGAGTTTGAGAGGTAGCCCAAAGGGGGCATTCATTGTCTCTTTATTATAATTGCCAATTCTTCTTCCAATCTGTTTACAAAGTTGAATAAAGGGGATTCAAGTTGATTTTGGTATGATTTTGGTGGTTAGTATAACCTTCTTCTGATAGGCCTAAATGTTATTTAAAGACTTTTGTAAAGATCTTTTATAGTATGACACTACAGGCTC
SEQUENCE_INCLUDED_REGION=34,303
P3_COMMENT=CL is TTTCCTTTTCTCCCTTGGTT
P3_COMMENT=CR is TCAGAAGAAGGTTATACTAACCACC
P3_COMMENT=CM is GAGTGTGGGACACTAGATCAGC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.731753
PRIMER_LEFT_0_PENALTY=0.039531
PRIMER_RIGHT_0_PENALTY=0.692222
PRIMER_INTERNAL_0_PENALTY=1.696755
PRIMER_LEFT_0_SEQUENCE=CCTTTCCTTTTCTCCCTTGG
PRIMER_RIGHT_0_SEQUENCE=CCTTTGGGCTACCTCTCAAA
PRIMER_INTERNAL_0_SEQUENCE=TGTGGGACACTAGATCAGCAA
PRIMER_LEFT_0=68,20
PRIMER_RIGHT_0=152,20
PRIMER_INTERNAL_0=94,21
PRIMER_LEFT_0_TM=60.040
PRIMER_RIGHT_0_TM=59.308
PRIMER_INTERNAL_0_TM=59.303
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_1_PENALTY=0.732229
PRIMER_LEFT_1_PENALTY=0.039531
PRIMER_RIGHT_1_PENALTY=0.692698
PRIMER_INTERNAL_1_PENALTY=1.696755
PRIMER_LEFT_1_SEQUENCE=CCTTTCCTTTTCTCCCTTGG
PRIMER_RIGHT_1_SEQUENCE=AGACAATGAATGCCCCCTTT
PRIMER_INTERNAL_1_SEQUENCE=TGTGGGACACTAGATCAGCAA
PRIMER_LEFT_1=68,20
PRIMER_RIGHT_1=167,20
PRIMER_INTERNAL_1=94,21
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=60.693
PRIMER_INTERNAL_1_TM=59.303
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=100
PRIMER_PAIR_2_PENALTY=0.733470
PRIMER_LEFT_2_PENALTY=0.040772
PRIMER_RIGHT_2_PENALTY=0.692698
PRIMER_INTERNAL_2_PENALTY=2.196745
PRIMER_LEFT_2_SEQUENCE=CCCTTGGTTAGAGTGTGGGA
PRIMER_RIGHT_2_SEQUENCE=AGACAATGAATGCCCCCTTT
PRIMER_INTERNAL_2_SEQUENCE=CATGAGTTTGAGAGGTAGCCC
PRIMER_LEFT_2=81,20
PRIMER_RIGHT_2=167,20
PRIMER_INTERNAL_2=127,21
PRIMER_LEFT_2_TM=59.959
PRIMER_RIGHT_2_TM=60.693
PRIMER_INTERNAL_2_TM=58.803
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=0.733470
PRIMER_LEFT_3_PENALTY=0.040772
PRIMER_RIGHT_3_PENALTY=0.692698
PRIMER_INTERNAL_3_PENALTY=2.196745
PRIMER_LEFT_3_SEQUENCE=TCCCTTGGTTAGAGTGTGGG
PRIMER_RIGHT_3_SEQUENCE=AGACAATGAATGCCCCCTTT
PRIMER_INTERNAL_3_SEQUENCE=CATGAGTTTGAGAGGTAGCCC
PRIMER_LEFT_3=80,20
PRIMER_RIGHT_3=167,20
PRIMER_INTERNAL_3=127,21
PRIMER_LEFT_3_TM=59.959
PRIMER_RIGHT_3_TM=60.693
PRIMER_INTERNAL_3_TM=58.803
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=0.893386
PRIMER_LEFT_4_PENALTY=0.040772
PRIMER_RIGHT_4_PENALTY=0.852615
PRIMER_INTERNAL_4_PENALTY=1.139192
PRIMER_LEFT_4_SEQUENCE=CCCTTGGTTAGAGTGTGGGA
PRIMER_RIGHT_4_SEQUENCE=AGAGACAATGAATGCCCCCT
PRIMER_INTERNAL_4_SEQUENCE=TGAGTTTGAGAGGTAGCCCAA
PRIMER_LEFT_4=81,20
PRIMER_RIGHT_4=169,20
PRIMER_INTERNAL_4=129,21
PRIMER_LEFT_4_TM=59.959
PRIMER_RIGHT_4_TM=60.853
PRIMER_INTERNAL_4_TM=59.861
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=89
=
SEQUENCE_ID=MH909
SEQUENCE_TEMPLATE=TCGACTCTAGAGGATCCCCCTAACTGCTCTAGTATNCTAGCCCTGAAAAACCACAAAGATGAGGATTAGCAGATGCTCAAGAAATATTTTTTGAATATGCTGGCTCTTATCTGCTCAGCAGTGTACAGATATGCTCTCACACTCGAGAAGCTACCAGAACAAACTGGTAGGCTCCCATTCTAGATGACATCTTTTCCCCATACTCTCAAAATGCAAGTTAACCCCCAACCCTGAGCAGAAGAAAGGAGAGGCCCCAGGTACCTGTCAATGAGGGAATCCCGCATGCTGGTGGCAATGGTGCTAGGCTGGGCTTCATTCAG
SEQUENCE_INCLUDED_REGION=19,301
P3_COMMENT=CL is CTAGCCCTGAAAAACCACAA
P3_COMMENT=CR is CTCATTGACAGGTACCTGGG
P3_COMMENT=CM is GATGAGGATTAGCAGATGCTCA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.124966
PRIMER_LEFT_0_PENALTY=0.095713
PRIMER_RIGHT_0_PENALTY=0.029253
PRIMER_INTERNAL_0_PENALTY=1.025669
PRIMER_LEFT_0_SEQUENCE=AGCAGAAGAAAGGAGAGGCC
PRIMER_RIGHT_0_SEQUENCE=CTGAATGAAGCCCAGCCTAG
PRIMER_INTERNAL_0_SEQUENCE=CAGGTACCTGTCAATGAGGGA
PRIMER_LEFT_0=233,20
PRIMER_RIGHT_0=319,20
PRIMER_INTERNAL_0=254,21
PRIMER_LEFT_0_TM=60.096
PRIMER_RIGHT_0_TM=59.971
PRIMER_INTERNAL_0_TM=59.974
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=0.241412
PRIMER_LEFT_1_PENALTY=0.088413
PRIMER_RIGHT_1_PENALTY=0.152999
PRIMER_INTERNAL_1_PENALTY=2.385416
PRIMER_LEFT_1_SEQUENCE=GCCCTGAAAAACCACAAAGA
PRIMER_RIGHT_1_SEQUENCE=TGAGCAGATAAGAGCCAGCA
PRIMER_INTERNAL_1_SEQUENCE=TGAGGATTAGCAGATGCTCAAG
PRIMER_LEFT_1=39,20
PRIMER_RIGHT_1=116,20
PRIMER_INTERNAL_1=59,22
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=59.847
PRIMER_INTERNAL_1_TM=59.615
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=78
PRIMER_PAIR_2_PENALTY=0.241412
PRIMER_LEFT_2_PENALTY=0.088413
PRIMER_RIGHT_2_PENALTY=0.152999
PRIMER_INTERNAL_2_PENALTY=2.385416
PRIMER_LEFT_2_SEQUENCE=GCCCTGAAAAACCACAAAGA
PRIMER_RIGHT_2_SEQUENCE=TGCTGAGCAGATAAGAGCCA
PRIMER_INTERNAL_2_SEQUENCE=TGAGGATTAGCAGATGCTCAAG
PRIMER_LEFT_2=39,20
PRIMER_RIGHT_2=119,20
PRIMER_INTERNAL_2=59,22
PRIMER_LEFT_2_TM=60.088
PRIMER_RIGHT_2_TM=59.847
PRIMER_INTERNAL_2_TM=59.615
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=0.395704
PRIMER_LEFT_3_PENALTY=0.366450
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_INTERNAL_3_PENALTY=0.769217
PRIMER_LEFT_3_SEQUENCE=CCAACCCTGAGCAGAAGAAA
PRIMER_RIGHT_3_SEQUENCE=CTGAATGAAGCCCAGCCTAG
PRIMER_INTERNAL_3_SEQUENCE=CCCCAGGTACCTGTCAATGA
PRIMER_LEFT_3=224,20
PRIMER_RIGHT_3=319,20
PRIMER_INTERNAL_3=251,20
PRIMER_LEFT_3_TM=60.366
PRIMER_RIGHT_3_TM=59.971
PRIMER_INTERNAL_3_TM=60.769
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=5.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=0.503654
PRIMER_LEFT_4_PENALTY=0.407941
PRIMER_RIGHT_4_PENALTY=0.095713
PRIMER_INTERNAL_4_PENALTY=0.684590
PRIMER_LEFT_4_SEQUENCE=AACAAACTGGTAGGCTCCCA
PRIMER_RIGHT_4_SEQUENCE=GGCCTCTCCTTTCTTCTGCT
PRIMER_INTERNAL_4_SEQUENCE=AAATGCAAGTTAACCCCCAA
PRIMER_LEFT_4=157,20
PRIMER_RIGHT_4=252,20
PRIMER_INTERNAL_4=208,20
PRIMER_LEFT_4_TM=59.592
PRIMER_RIGHT_4_TM=60.096
PRIMER_INTERNAL_4_TM=59.315
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=7.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=96
=
SEQUENCE_ID=MH915
SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTAAGATAAGAAGATACCACTGTAAAAAGGTTATTATTCCTAAAGAGGAACTAAACGTAGGCAAGGCCCCACTTTTCTGGAAGTGGCTTATCAGTGTGGATCCTAATAAGAACGAATGTNTAGCAATCACTTATATCCCAGACATGATTCTAAGTTCTCCATGTGCATTTACTCCTTTAATTCTCACAACACTCCTTGCAAGTGAGAAGTCTTATTATTTCCAAATTTCAGATACAGAAACTCAG
SEQUENCE_INCLUDED_REGION=31,245
P3_COMMENT=CL is ACCACTGTAAAAAGGTTATTATTCC
P3_COMMENT=CR is TGAGTTTCTGTATCTGAAATTTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.911049
PRIMER_LEFT_0_PENALTY=0.671597
PRIMER_RIGHT_0_PENALTY=3.239452
PRIMER_INTERNAL_0_PENALTY=4.439481
PRIMER_LEFT_0_SEQUENCE=CCACTTTTCTGGAAGTGGCT
PRIMER_RIGHT_0_SEQUENCE=TGCACATGGAGAACTTAGAATCA
PRIMER_INTERNAL_0_SEQUENCE=CAGTGTGGATCCTAATAAGAACGA
PRIMER_LEFT_0=99,20
PRIMER_RIGHT_0=197,23
PRIMER_INTERNAL_0=122,24
PRIMER_LEFT_0_TM=59.328
PRIMER_RIGHT_0_TM=59.761
PRIMER_INTERNAL_0_TM=59.561
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_INTERNAL_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=4.407680
PRIMER_LEFT_1_PENALTY=1.393460
PRIMER_RIGHT_1_PENALTY=3.014220
PRIMER_INTERNAL_1_PENALTY=4.236882
PRIMER_LEFT_1_SEQUENCE=GGAAGTGGCTTATCAGTGTGG
PRIMER_RIGHT_1_SEQUENCE=AAGGAGTAAATGCACATGGAGAA
PRIMER_INTERNAL_1_SEQUENCE=GCAATCACTTATATCCCAGACATG
PRIMER_LEFT_1=109,21
PRIMER_RIGHT_1=207,23
PRIMER_INTERNAL_1=153,24
PRIMER_LEFT_1_TM=59.607
PRIMER_RIGHT_1_TM=60.014
PRIMER_INTERNAL_1_TM=59.763
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_INTERNAL_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=4.407680
PRIMER_LEFT_2_PENALTY=1.393460
PRIMER_RIGHT_2_PENALTY=3.014220
PRIMER_INTERNAL_2_PENALTY=4.236882
PRIMER_LEFT_2_SEQUENCE=GGAAGTGGCTTATCAGTGTGG
PRIMER_RIGHT_2_SEQUENCE=AAAGGAGTAAATGCACATGGAGA
PRIMER_INTERNAL_2_SEQUENCE=GCAATCACTTATATCCCAGACATG
PRIMER_LEFT_2=109,21
PRIMER_RIGHT_2=208,23
PRIMER_INTERNAL_2=153,24
PRIMER_LEFT_2_TM=59.607
PRIMER_RIGHT_2_TM=60.014
PRIMER_INTERNAL_2_TM=59.763
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=4.449466
PRIMER_LEFT_3_PENALTY=1.435246
PRIMER_RIGHT_3_PENALTY=3.014220
PRIMER_INTERNAL_3_PENALTY=4.236882
PRIMER_LEFT_3_SEQUENCE=TGGCTTATCAGTGTGGATCCT
PRIMER_RIGHT_3_SEQUENCE=AAGGAGTAAATGCACATGGAGAA
PRIMER_INTERNAL_3_SEQUENCE=GCAATCACTTATATCCCAGACATG
PRIMER_LEFT_3=114,21
PRIMER_RIGHT_3=207,23
PRIMER_INTERNAL_3=153,24
PRIMER_LEFT_3_TM=59.565
PRIMER_RIGHT_3_TM=60.014
PRIMER_INTERNAL_3_TM=59.763
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_INTERNAL_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=4.449466
PRIMER_LEFT_4_PENALTY=1.435246
PRIMER_RIGHT_4_PENALTY=3.014220
PRIMER_INTERNAL_4_PENALTY=4.236882
PRIMER_LEFT_4_SEQUENCE=TGGCTTATCAGTGTGGATCCT
PRIMER_RIGHT_4_SEQUENCE=AAAGGAGTAAATGCACATGGAGA
PRIMER_INTERNAL_4_SEQUENCE=GCAATCACTTATATCCCAGACATG
PRIMER_LEFT_4=114,21
PRIMER_RIGHT_4=208,23
PRIMER_INTERNAL_4=153,24
PRIMER_LEFT_4_TM=59.565
PRIMER_RIGHT_4_TM=60.014
PRIMER_INTERNAL_4_TM=59.763
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_INTERNAL_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH917
SEQUENCE_TEMPLATE=TCATGCTCAGGTCGACTCTAGAGGATCCCCCTGGGGGTAAAGGTGGGAGGCAGTAGATGGATATAAAAGACCAGTGCTGCTCTCCCCCAAGTCCCCTCCTTTACCATTGCTTTCCTATGAATATTTTAAAGCAAAAGTAAAATAAGAATACCTAGGGCTTAATTTTTAAAGAGGGCACCTCATTTTATCCAAACCTCAGTAGGTACAGACCTGCTGCTTGGACAATATCACCTCAGCAGAGGCAACACTTCTGATGGCCACACTCCTAAATTTTAGTAAAAATACCTAAGAGGAAGCAATGAA
SEQUENCE_INCLUDED_REGION=30,273
P3_COMMENT=CL is TGGGAGGCAGTAGATGGATA
P3_COMMENT=CR is ATTTAGGAGTGTGGCCATCA
P3_COMMENT=CM is AAAAGACCAGTGCTGCTCTC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.294966
PRIMER_LEFT_0_PENALTY=0.238079
PRIMER_RIGHT_0_PENALTY=0.056887
PRIMER_INTERNAL_0_PENALTY=0.125826
PRIMER_LEFT_0_SEQUENCE=GGGCACCTCATTTTATCCAA
PRIMER_RIGHT_0_SEQUENCE=AGTGTTGCCTCTGCTGAGGT
PRIMER_INTERNAL_0_SEQUENCE=AGACCTGCTGCTTGGACAAT
PRIMER_LEFT_0=172,20
PRIMER_RIGHT_0=248,20
PRIMER_INTERNAL_0=206,20
PRIMER_LEFT_0_TM=59.762
PRIMER_RIGHT_0_TM=60.057
PRIMER_INTERNAL_0_TM=59.874
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=6.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=77
PRIMER_PAIR_1_PENALTY=0.357888
PRIMER_LEFT_1_PENALTY=0.238079
PRIMER_RIGHT_1_PENALTY=0.119809
PRIMER_INTERNAL_1_PENALTY=0.125826
PRIMER_LEFT_1_SEQUENCE=GGGCACCTCATTTTATCCAA
PRIMER_RIGHT_1_SEQUENCE=GGAGTGTGGCCATCAGAAGT
PRIMER_INTERNAL_1_SEQUENCE=AGACCTGCTGCTTGGACAAT
PRIMER_LEFT_1=172,20
PRIMER_RIGHT_1=265,20
PRIMER_INTERNAL_1=206,20
PRIMER_LEFT_1_TM=59.762
PRIMER_RIGHT_1_TM=60.120
PRIMER_INTERNAL_1_TM=59.874
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=94
PRIMER_PAIR_2_PENALTY=0.468489
PRIMER_LEFT_2_PENALTY=0.238079
PRIMER_RIGHT_2_PENALTY=0.230410
PRIMER_INTERNAL_2_PENALTY=0.125826
PRIMER_LEFT_2_SEQUENCE=GGGCACCTCATTTTATCCAA
PRIMER_RIGHT_2_SEQUENCE=CAGAAGTGTTGCCTCTGCTG
PRIMER_INTERNAL_2_SEQUENCE=AGACCTGCTGCTTGGACAAT
PRIMER_LEFT_2=172,20
PRIMER_RIGHT_2=252,20
PRIMER_INTERNAL_2=206,20
PRIMER_LEFT_2_TM=59.762
PRIMER_RIGHT_2_TM=59.770
PRIMER_INTERNAL_2_TM=59.874
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=81
PRIMER_PAIR_3_PENALTY=0.498835
PRIMER_LEFT_3_PENALTY=0.238079
PRIMER_RIGHT_3_PENALTY=0.260757
PRIMER_INTERNAL_3_PENALTY=0.125826
PRIMER_LEFT_3_SEQUENCE=GGGCACCTCATTTTATCCAA
PRIMER_RIGHT_3_SEQUENCE=AGGAGTGTGGCCATCAGAAG
PRIMER_INTERNAL_3_SEQUENCE=AGACCTGCTGCTTGGACAAT
PRIMER_LEFT_3=172,20
PRIMER_RIGHT_3=266,20
PRIMER_INTERNAL_3=206,20
PRIMER_LEFT_3_TM=59.762
PRIMER_RIGHT_3_TM=60.261
PRIMER_INTERNAL_3_TM=59.874
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=95
PRIMER_PAIR_4_PENALTY=0.549862
PRIMER_LEFT_4_PENALTY=0.311784
PRIMER_RIGHT_4_PENALTY=0.238079
PRIMER_INTERNAL_4_PENALTY=8.558113
PRIMER_LEFT_4_SEQUENCE=CCCCTCCTTTACCATTGCTT
PRIMER_RIGHT_4_SEQUENCE=TTGGATAAAATGAGGTGCCC
PRIMER_INTERNAL_4_SEQUENCE=CAAAAGTAAAATAAGAATACCTAGGGC
PRIMER_LEFT_4=92,20
PRIMER_RIGHT_4=191,20
PRIMER_INTERNAL_4=131,27
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.762
PRIMER_INTERNAL_4_TM=58.442
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=100
=
SEQUENCE_ID=MH918
SEQUENCE_TEMPLATE=ATGCTCAACCACTCTCCTCTAACAGCCCTGTGAACTTCCTCTGGCTTAGAATACATTGACCTGACTCTGTTGTAATTGCCGGGTTTTTNATTTGTCTTCTCCATTAGACCTCGGTTTCCAAATCTCGGCACAATTGACATCTTGGGAAAACTCACTCTTGGCTTGGGTGGGTGATAAGGGACTGTCCTGTGCATTT
SEQUENCE_INCLUDED_REGION=0,196
P3_COMMENT=CL is TCTCCTCTAACAGCCCTGTG
P3_COMMENT=CR is ACAGGACAGTCCCTTATCACC
P3_COMMENT=CM is ACTTCCTCTGGCTTAGAATACATTG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.239244
PRIMER_LEFT_0_PENALTY=0.006945
PRIMER_RIGHT_0_PENALTY=0.232299
PRIMER_INTERNAL_0_PENALTY=0.074208
PRIMER_LEFT_0_SEQUENCE=CTCTGTTGTAATTGCCGGGT
PRIMER_RIGHT_0_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
PRIMER_INTERNAL_0_SEQUENCE=CCATTAGACCTCGGTTTCCA
PRIMER_LEFT_0=64,20
PRIMER_RIGHT_0=161,20
PRIMER_INTERNAL_0=100,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.232
PRIMER_INTERNAL_0_TM=59.926
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=11.1000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=0.239244
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.232299
PRIMER_INTERNAL_1_PENALTY=0.074208
PRIMER_LEFT_1_SEQUENCE=ACTCTGTTGTAATTGCCGGG
PRIMER_RIGHT_1_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
PRIMER_INTERNAL_1_SEQUENCE=CCATTAGACCTCGGTTTCCA
PRIMER_LEFT_1=63,20
PRIMER_RIGHT_1=161,20
PRIMER_INTERNAL_1=100,20
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=60.232
PRIMER_INTERNAL_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=0.295053
PRIMER_LEFT_2_PENALTY=0.074208
PRIMER_RIGHT_2_PENALTY=0.220845
PRIMER_INTERNAL_2_PENALTY=0.232299
PRIMER_LEFT_2_SEQUENCE=CCATTAGACCTCGGTTTCCA
PRIMER_RIGHT_2_SEQUENCE=GGACAGTCCCTTATCACCCA
PRIMER_INTERNAL_2_SEQUENCE=TGGGAAAACTCACTCTTGGC
PRIMER_LEFT_2=100,20
PRIMER_RIGHT_2=186,20
PRIMER_INTERNAL_2=142,20
PRIMER_LEFT_2_TM=59.926
PRIMER_RIGHT_2_TM=59.779
PRIMER_INTERNAL_2_TM=60.232
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=0.371428
PRIMER_LEFT_3_PENALTY=0.139129
PRIMER_RIGHT_3_PENALTY=0.232299
PRIMER_INTERNAL_3_PENALTY=0.074208
PRIMER_LEFT_3_SEQUENCE=CTGTTGTAATTGCCGGGTTT
PRIMER_RIGHT_3_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
PRIMER_INTERNAL_3_SEQUENCE=CCATTAGACCTCGGTTTCCA
PRIMER_LEFT_3=66,20
PRIMER_RIGHT_3=161,20
PRIMER_INTERNAL_3=100,20
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=60.232
PRIMER_INTERNAL_3_TM=59.926
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=96
PRIMER_PAIR_4_PENALTY=0.455048
PRIMER_LEFT_4_PENALTY=0.222749
PRIMER_RIGHT_4_PENALTY=0.232299
PRIMER_INTERNAL_4_PENALTY=0.074208
PRIMER_LEFT_4_SEQUENCE=TGTTGTAATTGCCGGGTTTT
PRIMER_RIGHT_4_SEQUENCE=GCCAAGAGTGAGTTTTCCCA
PRIMER_INTERNAL_4_SEQUENCE=CCATTAGACCTCGGTTTCCA
PRIMER_LEFT_4=67,20
PRIMER_RIGHT_4=161,20
PRIMER_INTERNAL_4=100,20
PRIMER_LEFT_4_TM=60.223
PRIMER_RIGHT_4_TM=60.232
PRIMER_INTERNAL_4_TM=59.926
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=95
=
SEQUENCE_ID=MH919
SEQUENCE_TEMPLATE=NNTTCANTNCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGTAGCCTTTAAACTAGTGTAGCACTGAAGTATGTTTGCTGTGTTTAGTTTANTATAATTGAATTTTTAACTTAAAATCTTACTGTCTTATTTGGAAGAAGCACCAGTTCAAAGCACATAGGTCTACGTTGATAAATGAATAACGCTGATGTTAAGCACATTTATTATGAATGTNATTTCTCAAGATGCACCTGCTGCAGCGGAGAAGAATCAGTCCTGGTCAAAGTGAGCAGTAGGTAGAG
SEQUENCE_INCLUDED_REGION=35,247
P3_COMMENT=CL is AGTGTAGCACTGAAGTATGTTTGC
P3_COMMENT=CR is AGGACTGATTCTTCTCCGCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.645925
PRIMER_LEFT_0_PENALTY=0.509923
PRIMER_RIGHT_0_PENALTY=0.136001
PRIMER_INTERNAL_0_PENALTY=3.163489
PRIMER_LEFT_0_SEQUENCE=AAGCACCAGTTCAAAGCACA
PRIMER_RIGHT_0_SEQUENCE=CAGCAGGTGCATCTTGAGAA
PRIMER_INTERNAL_0_SEQUENCE=AACGCTGATGTTAAGCACATTT
PRIMER_LEFT_0=138,20
PRIMER_RIGHT_0=236,20
PRIMER_INTERNAL_0=181,22
PRIMER_LEFT_0_TM=59.490
PRIMER_RIGHT_0_TM=60.136
PRIMER_INTERNAL_0_TM=58.837
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=36.364
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=1.341407
PRIMER_LEFT_1_PENALTY=1.205406
PRIMER_RIGHT_1_PENALTY=0.136001
PRIMER_INTERNAL_1_PENALTY=3.163489
PRIMER_LEFT_1_SEQUENCE=AAGCACCAGTTCAAAGCACAT
PRIMER_RIGHT_1_SEQUENCE=CAGCAGGTGCATCTTGAGAA
PRIMER_INTERNAL_1_SEQUENCE=AACGCTGATGTTAAGCACATTT
PRIMER_LEFT_1=138,21
PRIMER_RIGHT_1=236,20
PRIMER_INTERNAL_1=181,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=60.136
PRIMER_INTERNAL_1_TM=58.837
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=36.364
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=99
PRIMER_PAIR_2_PENALTY=1.637950
PRIMER_LEFT_2_PENALTY=1.501949
PRIMER_RIGHT_2_PENALTY=0.136001
PRIMER_INTERNAL_2_PENALTY=3.163489
PRIMER_LEFT_2_SEQUENCE=GAAGCACCAGTTCAAAGCAC
PRIMER_RIGHT_2_SEQUENCE=CAGCAGGTGCATCTTGAGAA
PRIMER_INTERNAL_2_SEQUENCE=AACGCTGATGTTAAGCACATTT
PRIMER_LEFT_2=137,20
PRIMER_RIGHT_2=236,20
PRIMER_INTERNAL_2=181,22
PRIMER_LEFT_2_TM=58.498
PRIMER_RIGHT_2_TM=60.136
PRIMER_INTERNAL_2_TM=58.837
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=36.364
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=100
PRIMER_PAIR_3_PENALTY=1.764958
PRIMER_LEFT_3_PENALTY=1.628957
PRIMER_RIGHT_3_PENALTY=0.136001
PRIMER_INTERNAL_3_PENALTY=3.163489
PRIMER_LEFT_3_SEQUENCE=AGCACCAGTTCAAAGCACAT
PRIMER_RIGHT_3_SEQUENCE=CAGCAGGTGCATCTTGAGAA
PRIMER_INTERNAL_3_SEQUENCE=AACGCTGATGTTAAGCACATTT
PRIMER_LEFT_3=139,20
PRIMER_RIGHT_3=236,20
PRIMER_INTERNAL_3=181,22
PRIMER_LEFT_3_TM=58.371
PRIMER_RIGHT_3_TM=60.136
PRIMER_INTERNAL_3_TM=58.837
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=36.364
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=98
PRIMER_PAIR_4_PENALTY=1.912469
PRIMER_LEFT_4_PENALTY=0.509923
PRIMER_RIGHT_4_PENALTY=1.402545
PRIMER_INTERNAL_4_PENALTY=3.163489
PRIMER_LEFT_4_SEQUENCE=AAGCACCAGTTCAAAGCACA
PRIMER_RIGHT_4_SEQUENCE=AGCAGGTGCATCTTGAGAAA
PRIMER_INTERNAL_4_SEQUENCE=AACGCTGATGTTAAGCACATTT
PRIMER_LEFT_4=138,20
PRIMER_RIGHT_4=235,20
PRIMER_INTERNAL_4=181,22
PRIMER_LEFT_4_TM=59.490
PRIMER_RIGHT_4_TM=58.597
PRIMER_INTERNAL_4_TM=58.837
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=36.364
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=98
=
SEQUENCE_ID=MH936
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGCCTTTAGCCCCTCTCCCTTCACTGGAGGTTGGGGCTGGCGGGAGGGGTGGGCCTGAAACTTCCAACCCTCTAATTAGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT
SEQUENCE_INCLUDED_REGION=36,82
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.255193
PRIMER_LEFT_0_PENALTY=1.483058
PRIMER_RIGHT_0_PENALTY=1.772136
PRIMER_INTERNAL_0_PENALTY=2.985442
PRIMER_LEFT_0_SEQUENCE=TCAGCCTTTAGCCCCTCTC
PRIMER_RIGHT_0_SEQUENCE=AGAGGGTTGGAAGTTTCAGG
PRIMER_INTERNAL_0_SEQUENCE=CTTCACTGGAGGTTGGGG
PRIMER_LEFT_0=36,19
PRIMER_RIGHT_0=110,20
PRIMER_INTERNAL_0=56,18
PRIMER_LEFT_0_TM=59.517
PRIMER_RIGHT_0_TM=58.228
PRIMER_INTERNAL_0_TM=59.015
PRIMER_LEFT_0_GC_PERCENT=57.895
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=3.856833
PRIMER_LEFT_1_PENALTY=1.483058
PRIMER_RIGHT_1_PENALTY=2.373775
PRIMER_INTERNAL_1_PENALTY=2.985442
PRIMER_LEFT_1_SEQUENCE=TCAGCCTTTAGCCCCTCTC
PRIMER_RIGHT_1_SEQUENCE=TTAGAGGGTTGGAAGTTTCAGG
PRIMER_INTERNAL_1_SEQUENCE=CTTCACTGGAGGTTGGGG
PRIMER_LEFT_1=36,19
PRIMER_RIGHT_1=112,22
PRIMER_INTERNAL_1=56,18
PRIMER_LEFT_1_TM=59.517
PRIMER_RIGHT_1_TM=59.626
PRIMER_INTERNAL_1_TM=59.015
PRIMER_LEFT_1_GC_PERCENT=57.895
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=4.178092
PRIMER_LEFT_2_PENALTY=1.483058
PRIMER_RIGHT_2_PENALTY=2.695034
PRIMER_INTERNAL_2_PENALTY=2.985442
PRIMER_LEFT_2_SEQUENCE=TCAGCCTTTAGCCCCTCTC
PRIMER_RIGHT_2_SEQUENCE=TAGAGGGTTGGAAGTTTCAGG
PRIMER_INTERNAL_2_SEQUENCE=CTTCACTGGAGGTTGGGG
PRIMER_LEFT_2=36,19
PRIMER_RIGHT_2=111,21
PRIMER_INTERNAL_2=56,18
PRIMER_LEFT_2_TM=59.517
PRIMER_RIGHT_2_TM=58.305
PRIMER_INTERNAL_2_TM=59.015
PRIMER_LEFT_2_GC_PERCENT=57.895
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=76
PRIMER_PAIR_3_PENALTY=4.352208
PRIMER_LEFT_3_PENALTY=1.483058
PRIMER_RIGHT_3_PENALTY=2.869150
PRIMER_INTERNAL_3_PENALTY=2.985442
PRIMER_LEFT_3_SEQUENCE=TCAGCCTTTAGCCCCTCTC
PRIMER_RIGHT_3_SEQUENCE=AGAGGGTTGGAAGTTTCAGGC
PRIMER_INTERNAL_3_SEQUENCE=CTTCACTGGAGGTTGGGG
PRIMER_LEFT_3=36,19
PRIMER_RIGHT_3=110,21
PRIMER_INTERNAL_3=56,18
PRIMER_LEFT_3_TM=59.517
PRIMER_RIGHT_3_TM=61.869
PRIMER_INTERNAL_3_TM=59.015
PRIMER_LEFT_3_GC_PERCENT=57.895
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_INTERNAL_3_GC_PERCENT=61.111
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=75
PRIMER_PAIR_4_PENALTY=4.594966
PRIMER_LEFT_4_PENALTY=1.483058
PRIMER_RIGHT_4_PENALTY=3.111908
PRIMER_INTERNAL_4_PENALTY=2.985442
PRIMER_LEFT_4_SEQUENCE=TCAGCCTTTAGCCCCTCTC
PRIMER_RIGHT_4_SEQUENCE=ATTAGAGGGTTGGAAGTTTCAGG
PRIMER_INTERNAL_4_SEQUENCE=CTTCACTGGAGGTTGGGG
PRIMER_LEFT_4=36,19
PRIMER_RIGHT_4=113,23
PRIMER_INTERNAL_4=56,18
PRIMER_LEFT_4_TM=59.517
PRIMER_RIGHT_4_TM=59.888
PRIMER_INTERNAL_4_TM=59.015
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_INTERNAL_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=78
=
SEQUENCE_ID=MH952
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCTGTTCAGGTACCCCTTCCAGAGAAGCCAGGAGCACCCGGCGTCCCANACAAGTAAGTAAGCGCTGGGCGGGGGCCTGGCGCTCGCNGGGGCCGCCTCATTCCGGGGTCGCCTCATTCCAGGGCCGCCTCGCGAG
SEQUENCE_INCLUDED_REGION=36,137
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MH958
SEQUENCE_TEMPLATE=TTGCTCTGCCTGGAGCATCCTCTCTGACGTGGCTCTGTGATTCACTTTGCGCCCCTCCTCAGGTCTCTTCTTCTCCGTTACCTCCCTAGACAGGTTTTCCCTCTGCCCTGCTCTCAATCACTCACTAGTGTTATTTTTCTTCATGCCATTTGTATTCTCTCCTGTCTTCAATTGCTTATTGCTTATGTGTTTATTGTTACCCACCCCAACTGGAATGAAAGATCCATGCAGACAAGGACTTTGTTTTTGCTCACTGCAGTGACTCCACCACCTAGAACAGTGCTTGGTG
SEQUENCE_INCLUDED_REGION=0,289
P3_COMMENT=CL is CTTCTTCTCCGTTACCTCCC
P3_COMMENT=CR is AGCACTGTTCTAGGTGGTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.683573
PRIMER_LEFT_0_PENALTY=0.055279
PRIMER_RIGHT_0_PENALTY=0.628294
PRIMER_INTERNAL_0_PENALTY=0.199123
PRIMER_LEFT_0_SEQUENCE=CCTGGAGCATCCTCTCTGAC
PRIMER_RIGHT_0_SEQUENCE=GGCAGAGGGAAAACCTGTCT
PRIMER_INTERNAL_0_SEQUENCE=CCCCTCCTCAGGTCTCTTCT
PRIMER_LEFT_0=8,20
PRIMER_RIGHT_0=106,20
PRIMER_INTERNAL_0=51,20
PRIMER_LEFT_0_TM=59.945
PRIMER_RIGHT_0_TM=60.628
PRIMER_INTERNAL_0_TM=59.801
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=99
PRIMER_PAIR_1_PENALTY=0.740888
PRIMER_LEFT_1_PENALTY=0.431486
PRIMER_RIGHT_1_PENALTY=0.309402
PRIMER_INTERNAL_1_PENALTY=0.711868
PRIMER_LEFT_1_SEQUENCE=ATTGTTACCCACCCCAACTG
PRIMER_RIGHT_1_SEQUENCE=TAGGTGGTGGAGTCACTGCA
PRIMER_INTERNAL_1_SEQUENCE=CCATGCAGACAAGGACTTTG
PRIMER_LEFT_1=192,20
PRIMER_RIGHT_1=273,20
PRIMER_INTERNAL_1=223,20
PRIMER_LEFT_1_TM=59.569
PRIMER_RIGHT_1_TM=60.309
PRIMER_INTERNAL_1_TM=59.288
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=7.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.871517
PRIMER_LEFT_2_PENALTY=0.243223
PRIMER_RIGHT_2_PENALTY=0.628294
PRIMER_INTERNAL_2_PENALTY=0.199123
PRIMER_LEFT_2_SEQUENCE=CTCTCTGACGTGGCTCTGTG
PRIMER_RIGHT_2_SEQUENCE=GGCAGAGGGAAAACCTGTCT
PRIMER_INTERNAL_2_SEQUENCE=CCCCTCCTCAGGTCTCTTCT
PRIMER_LEFT_2=19,20
PRIMER_RIGHT_2=106,20
PRIMER_INTERNAL_2=51,20
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=60.628
PRIMER_INTERNAL_2_TM=59.801
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=0.958438
PRIMER_LEFT_3_PENALTY=0.403673
PRIMER_RIGHT_3_PENALTY=0.554765
PRIMER_INTERNAL_3_PENALTY=1.301100
PRIMER_LEFT_3_SEQUENCE=CTCTGTGATTCACTTTGCGC
PRIMER_RIGHT_3_SEQUENCE=AGTGATTGAGAGCAGGGCAG
PRIMER_INTERNAL_3_SEQUENCE=TCTTCTTCTCCGTTACCTCCC
PRIMER_LEFT_3=32,20
PRIMER_RIGHT_3=121,20
PRIMER_INTERNAL_3=65,21
PRIMER_LEFT_3_TM=59.596
PRIMER_RIGHT_3_TM=60.555
PRIMER_INTERNAL_3_TM=59.699
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=0.958438
PRIMER_LEFT_4_PENALTY=0.403673
PRIMER_RIGHT_4_PENALTY=0.554765
PRIMER_INTERNAL_4_PENALTY=0.199123
PRIMER_LEFT_4_SEQUENCE=GCTCTGTGATTCACTTTGCG
PRIMER_RIGHT_4_SEQUENCE=AGTGATTGAGAGCAGGGCAG
PRIMER_INTERNAL_4_SEQUENCE=CCCCTCCTCAGGTCTCTTCT
PRIMER_LEFT_4=31,20
PRIMER_RIGHT_4=121,20
PRIMER_INTERNAL_4=51,20
PRIMER_LEFT_4_TM=59.596
PRIMER_RIGHT_4_TM=60.555
PRIMER_INTERNAL_4_TM=59.801
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=91
=
SEQUENCE_ID=MH959
SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTAAACAAACCTGCAGTAGAAGCAAGCCCTCTCCAGGAAATCCCAATCTCTTTCCAAACAGGGACAGCAAAGGTTCTCTCTGTTGTGGCTGTTTTTCCCTCTCTTCTGGGAATTGGTTTGCCTTCTGAGAGCATCCCAGGAAGGGGAGGTGCGCCCCAAATTGGCGAGGCTCTGCAAATACCAAAGGCTCTTCTTTGGAAGAAGACTCCTCCTTTGGACGTGAGGCGCCCCTACCAGAGCGCCCGCNTACAGAAACGGAAACCACTAGCTACCTGGGAGG
SEQUENCE_INCLUDED_REGION=33,283
P3_COMMENT=CL is AGTAGAAGCAAGCCCTCTCC
P3_COMMENT=CR is ACGTCCAAAGGAGGAGTCTT
P3_COMMENT=CM is GGAAATCCCAATCTCTTTCC
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.199972
PRIMER_LEFT_0_PENALTY=0.051345
PRIMER_RIGHT_0_PENALTY=0.148627
PRIMER_INTERNAL_0_PENALTY=2.672380
PRIMER_LEFT_0_SEQUENCE=GGAATTGGTTTGCCTTCTGA
PRIMER_RIGHT_0_SEQUENCE=GCCTTTGGTATTTGCAGAGC
PRIMER_INTERNAL_0_SEQUENCE=ATCCCAGGAAGGGGAGGT
PRIMER_LEFT_0=144,20
PRIMER_RIGHT_0=223,20
PRIMER_INTERNAL_0=168,18
PRIMER_LEFT_0_TM=60.051
PRIMER_RIGHT_0_TM=59.851
PRIMER_INTERNAL_0_TM=60.672
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=0.199972
PRIMER_LEFT_1_PENALTY=0.051345
PRIMER_RIGHT_1_PENALTY=0.148627
PRIMER_INTERNAL_1_PENALTY=0.487936
PRIMER_LEFT_1_SEQUENCE=TCTTCTGGGAATTGGTTTGC
PRIMER_RIGHT_1_SEQUENCE=GCCTTTGGTATTTGCAGAGC
PRIMER_INTERNAL_1_SEQUENCE=TCTGAGAGCATCCCAGGAAG
PRIMER_LEFT_1=137,20
PRIMER_RIGHT_1=223,20
PRIMER_INTERNAL_1=159,20
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=59.851
PRIMER_INTERNAL_1_TM=60.488
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=0.283644
PRIMER_LEFT_2_PENALTY=0.232299
PRIMER_RIGHT_2_PENALTY=0.051345
PRIMER_INTERNAL_2_PENALTY=0.232299
PRIMER_LEFT_2_SEQUENCE=TCTTTCCAAACAGGGACAGC
PRIMER_RIGHT_2_SEQUENCE=TCAGAAGGCAAACCAATTCC
PRIMER_INTERNAL_2_SEQUENCE=TGTGGCTGTTTTTCCCTCTC
PRIMER_LEFT_2=84,20
PRIMER_RIGHT_2=163,20
PRIMER_INTERNAL_2=119,20
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=60.051
PRIMER_INTERNAL_2_TM=60.232
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=80
PRIMER_PAIR_3_PENALTY=0.314950
PRIMER_LEFT_3_PENALTY=0.263605
PRIMER_RIGHT_3_PENALTY=0.051345
PRIMER_INTERNAL_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=CCTCTCCAGGAAATCCCAAT
PRIMER_RIGHT_3_SEQUENCE=GCAAACCAATTCCCAGAAGA
PRIMER_INTERNAL_3_SEQUENCE=CAAACAGGGACAGCAAAGGT
PRIMER_LEFT_3=63,20
PRIMER_RIGHT_3=156,20
PRIMER_INTERNAL_3=90,20
PRIMER_LEFT_3_TM=60.264
PRIMER_RIGHT_3_TM=60.051
PRIMER_INTERNAL_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=94
PRIMER_PAIR_4_PENALTY=0.343759
PRIMER_LEFT_4_PENALTY=0.051345
PRIMER_RIGHT_4_PENALTY=0.292414
PRIMER_INTERNAL_4_PENALTY=2.672380
PRIMER_LEFT_4_SEQUENCE=GGAATTGGTTTGCCTTCTGA
PRIMER_RIGHT_4_SEQUENCE=CCTTTGGTATTTGCAGAGCC
PRIMER_INTERNAL_4_SEQUENCE=ATCCCAGGAAGGGGAGGT
PRIMER_LEFT_4=144,20
PRIMER_RIGHT_4=222,20
PRIMER_INTERNAL_4=168,18
PRIMER_LEFT_4_TM=60.051
PRIMER_RIGHT_4_TM=59.708
PRIMER_INTERNAL_4_TM=60.672
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=61.111
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH963
SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTCTTTTCATGCTGTATCATTTTTAATAACTCAGTTAAAAGTTGTCTTTTTAGAATGCCTTCTTATGGTNTTATGATCTGGCCTCACTTCGCGAATACTAATTGAAAGAGCCAAGATATGGTTACAGAAGGTCCAGATGAATGGAGATGTGTCACTAGCAGAACAGAGTTGACTTTCTCCATGGGAATCTATCCCTGAAACAAAGGGACTGTCACTTCAATGACCCATGAGCCTCAGTTAGTCACTAAGCAGACATATTTGAGAAACATGCCTTATCAATTACTTCAGGAAATAAGTATTTTTAAAAAATGAAAATTTATTAACT
SEQUENCE_INCLUDED_REGION=34,328
P3_COMMENT=CL is GCCTCACTTCGCGAATACTA
P3_COMMENT=CR is TATTTCCTGAAGTAATTGATAAGGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.138382
PRIMER_LEFT_0_PENALTY=1.057653
PRIMER_RIGHT_0_PENALTY=0.080729
PRIMER_INTERNAL_0_PENALTY=1.873692
PRIMER_LEFT_0_SEQUENCE=CAGAAGGTCCAGATGAATGGA
PRIMER_RIGHT_0_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
PRIMER_INTERNAL_0_SEQUENCE=GAGTTGACTTTCTCCATGGGA
PRIMER_LEFT_0=161,21
PRIMER_RIGHT_0=247,20
PRIMER_INTERNAL_0=202,21
PRIMER_LEFT_0_TM=60.058
PRIMER_RIGHT_0_TM=60.081
PRIMER_INTERNAL_0_TM=59.126
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=87
PRIMER_PAIR_1_PENALTY=2.001567
PRIMER_LEFT_1_PENALTY=1.920838
PRIMER_RIGHT_1_PENALTY=0.080729
PRIMER_INTERNAL_1_PENALTY=1.873692
PRIMER_LEFT_1_SEQUENCE=GAAGGTCCAGATGAATGGAGA
PRIMER_RIGHT_1_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
PRIMER_INTERNAL_1_SEQUENCE=GAGTTGACTTTCTCCATGGGA
PRIMER_LEFT_1=163,21
PRIMER_RIGHT_1=247,20
PRIMER_INTERNAL_1=202,21
PRIMER_LEFT_1_TM=59.079
PRIMER_RIGHT_1_TM=60.081
PRIMER_INTERNAL_1_TM=59.126
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=85
PRIMER_PAIR_2_PENALTY=2.009392
PRIMER_LEFT_2_PENALTY=1.928663
PRIMER_RIGHT_2_PENALTY=0.080729
PRIMER_INTERNAL_2_PENALTY=1.873692
PRIMER_LEFT_2_SEQUENCE=CAGAAGGTCCAGATGAATGG
PRIMER_RIGHT_2_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
PRIMER_INTERNAL_2_SEQUENCE=GAGTTGACTTTCTCCATGGGA
PRIMER_LEFT_2=161,20
PRIMER_RIGHT_2=247,20
PRIMER_INTERNAL_2=202,21
PRIMER_LEFT_2_TM=58.071
PRIMER_RIGHT_2_TM=60.081
PRIMER_INTERNAL_2_TM=59.126
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=87
PRIMER_PAIR_3_PENALTY=2.021147
PRIMER_LEFT_3_PENALTY=1.057653
PRIMER_RIGHT_3_PENALTY=0.963493
PRIMER_INTERNAL_3_PENALTY=1.873692
PRIMER_LEFT_3_SEQUENCE=CAGAAGGTCCAGATGAATGGA
PRIMER_RIGHT_3_SEQUENCE=ACAGTCCCTTTGTTTCAGGG
PRIMER_INTERNAL_3_SEQUENCE=GAGTTGACTTTCTCCATGGGA
PRIMER_LEFT_3=161,21
PRIMER_RIGHT_3=248,20
PRIMER_INTERNAL_3=202,21
PRIMER_LEFT_3_TM=60.058
PRIMER_RIGHT_3_TM=59.037
PRIMER_INTERNAL_3_TM=59.126
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=88
PRIMER_PAIR_4_PENALTY=2.077407
PRIMER_LEFT_4_PENALTY=1.996678
PRIMER_RIGHT_4_PENALTY=0.080729
PRIMER_INTERNAL_4_PENALTY=1.873692
PRIMER_LEFT_4_SEQUENCE=ACAGAAGGTCCAGATGAATGG
PRIMER_RIGHT_4_SEQUENCE=CAGTCCCTTTGTTTCAGGGA
PRIMER_INTERNAL_4_SEQUENCE=GAGTTGACTTTCTCCATGGGA
PRIMER_LEFT_4=160,21
PRIMER_RIGHT_4=247,20
PRIMER_INTERNAL_4=202,21
PRIMER_LEFT_4_TM=59.003
PRIMER_RIGHT_4_TM=60.081
PRIMER_INTERNAL_4_TM=59.126
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=88
=
SEQUENCE_ID=MH968
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACATATATGAAGTNAAGATGGCAGGTGCCAACCTGTCCACTTCTTGGCAGAAAATGATCTCAGTCACCAAGGGAAGGGGAGGCTCAGAGCAACTCTTCCTGGGAGTGTCCTTAGGACAATGACTGTTGGCTCACCTTGAAGTACACAGTGCCCCGCTCTTTCACTATGGTGCTCTGTTCCAG
SEQUENCE_INCLUDED_REGION=36,185
P3_COMMENT=CL is GTCCACTTCTTGGCAGAAAA
P3_COMMENT=CR is AGCACCATAGTGAAAGAGCG
P3_COMMENT=CM is GATCTCAGTCACCAAGGGAA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.509870
PRIMER_LEFT_0_PENALTY=0.158723
PRIMER_RIGHT_0_PENALTY=0.351148
PRIMER_INTERNAL_0_PENALTY=0.229181
PRIMER_LEFT_0_SEQUENCE=GTGCCAACCTGTCCACTTCT
PRIMER_RIGHT_0_SEQUENCE=TCCTAAGGACACTCCCAGGA
PRIMER_INTERNAL_0_SEQUENCE=TCTCAGTCACCAAGGGAAGG
PRIMER_LEFT_0=63,20
PRIMER_RIGHT_0=154,20
PRIMER_INTERNAL_0=96,20
PRIMER_LEFT_0_TM=60.159
PRIMER_RIGHT_0_TM=59.649
PRIMER_INTERNAL_0_TM=60.229
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=5.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=92
PRIMER_PAIR_1_PENALTY=0.547116
PRIMER_LEFT_1_PENALTY=0.229181
PRIMER_RIGHT_1_PENALTY=0.317935
PRIMER_INTERNAL_1_PENALTY=0.523038
PRIMER_LEFT_1_SEQUENCE=TCTCAGTCACCAAGGGAAGG
PRIMER_RIGHT_1_SEQUENCE=TGAGCCAACAGTCATTGTCC
PRIMER_INTERNAL_1_SEQUENCE=CAGAGCAACTCTTCCTGGGA
PRIMER_LEFT_1=96,20
PRIMER_RIGHT_1=171,20
PRIMER_INTERNAL_1=123,20
PRIMER_LEFT_1_TM=60.229
PRIMER_RIGHT_1_TM=59.682
PRIMER_INTERNAL_1_TM=60.523
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=76
PRIMER_PAIR_2_PENALTY=0.649166
PRIMER_LEFT_2_PENALTY=0.298018
PRIMER_RIGHT_2_PENALTY=0.351148
PRIMER_INTERNAL_2_PENALTY=0.229181
PRIMER_LEFT_2_SEQUENCE=ACCTGTCCACTTCTTGGCAG
PRIMER_RIGHT_2_SEQUENCE=TCCTAAGGACACTCCCAGGA
PRIMER_INTERNAL_2_SEQUENCE=TCTCAGTCACCAAGGGAAGG
PRIMER_LEFT_2=69,20
PRIMER_RIGHT_2=154,20
PRIMER_INTERNAL_2=96,20
PRIMER_LEFT_2_TM=60.298
PRIMER_RIGHT_2_TM=59.649
PRIMER_INTERNAL_2_TM=60.229
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=5.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=0.669140
PRIMER_LEFT_3_PENALTY=0.229181
PRIMER_RIGHT_3_PENALTY=0.439959
PRIMER_INTERNAL_3_PENALTY=0.351148
PRIMER_LEFT_3_SEQUENCE=TCTCAGTCACCAAGGGAAGG
PRIMER_RIGHT_3_SEQUENCE=TCAAGGTGAGCCAACAGTCA
PRIMER_INTERNAL_3_SEQUENCE=TCCTGGGAGTGTCCTTAGGA
PRIMER_LEFT_3=96,20
PRIMER_RIGHT_3=177,20
PRIMER_INTERNAL_3=135,20
PRIMER_LEFT_3_TM=60.229
PRIMER_RIGHT_3_TM=60.440
PRIMER_INTERNAL_3_TM=59.649
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=82
PRIMER_PAIR_4_PENALTY=0.681761
PRIMER_LEFT_4_PENALTY=0.158723
PRIMER_RIGHT_4_PENALTY=0.523038
PRIMER_INTERNAL_4_PENALTY=0.229181
PRIMER_LEFT_4_SEQUENCE=GTGCCAACCTGTCCACTTCT
PRIMER_RIGHT_4_SEQUENCE=CCCAGGAAGAGTTGCTCTGA
PRIMER_INTERNAL_4_SEQUENCE=TCTCAGTCACCAAGGGAAGG
PRIMER_LEFT_4=63,20
PRIMER_RIGHT_4=141,20
PRIMER_INTERNAL_4=96,20
PRIMER_LEFT_4_TM=60.159
PRIMER_RIGHT_4_TM=60.523
PRIMER_INTERNAL_4_TM=60.229
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH974
SEQUENCE_TEMPLATE=CTGAAGGAGAGGAACCTCCAAATACAAACCTTGTTGGTTTTCGTGATTCTTGTGGCATTCAATGTTAAATGCTTCTGCCCCGAGGCTTGTGGCAATTGGTTAATATTGATGTCAGTGCCCTTCCCTCCTGTGTATTTTCGCTGGGAAAGTTTGAGAGATAAGGGGAGTTGATGTGTACAACTTAGTTTGAAAAGAACTTGTTCTCTCCTAGCCACTGAAAACCGCGTTGCTTTCTGCTTCCCATTCTTCCTTTACTGATAATCTGGCCATCACTCTGCTGTTTAAAAGTCTTGCTAGAGTATGGAGACAAG
SEQUENCE_INCLUDED_REGION=0,311
P3_COMMENT=CL is GAAGGAGAGGAACCTCCAAA
P3_COMMENT=CR is GTCTCCATACTCTAGCAAGACTTT
P3_COMMENT=CM is ACAAACCTTGTTGGTTTTCG
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.297585
PRIMER_LEFT_0_PENALTY=0.131140
PRIMER_RIGHT_0_PENALTY=0.166445
PRIMER_INTERNAL_0_PENALTY=0.044832
PRIMER_LEFT_0_SEQUENCE=CTCCTAGCCACTGAAAACCG
PRIMER_RIGHT_0_SEQUENCE=AGCAGAGTGATGGCCAGATT
PRIMER_INTERNAL_0_SEQUENCE=TGCTTTCTGCTTCCCATTCT
PRIMER_LEFT_0=204,20
PRIMER_RIGHT_0=278,20
PRIMER_INTERNAL_0=227,20
PRIMER_LEFT_0_TM=59.869
PRIMER_RIGHT_0_TM=59.834
PRIMER_INTERNAL_0_TM=59.955
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.300641
PRIMER_LEFT_1_PENALTY=0.234379
PRIMER_RIGHT_1_PENALTY=0.066262
PRIMER_INTERNAL_1_PENALTY=0.501869
PRIMER_LEFT_1_SEQUENCE=TTTTCGTGATTCTTGTGGCA
PRIMER_RIGHT_1_SEQUENCE=GGAAGGGCACTGACATCAAT
PRIMER_INTERNAL_1_SEQUENCE=AGGCTTGTGGCAATTGGTTA
PRIMER_LEFT_1=37,20
PRIMER_RIGHT_1=123,20
PRIMER_INTERNAL_1=82,20
PRIMER_LEFT_1_TM=60.234
PRIMER_RIGHT_1_TM=59.934
PRIMER_INTERNAL_1_TM=60.502
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=87
PRIMER_PAIR_2_PENALTY=0.412026
PRIMER_LEFT_2_PENALTY=0.345764
PRIMER_RIGHT_2_PENALTY=0.066262
PRIMER_INTERNAL_2_PENALTY=0.501869
PRIMER_LEFT_2_SEQUENCE=TCGTGATTCTTGTGGCATTC
PRIMER_RIGHT_2_SEQUENCE=GGAAGGGCACTGACATCAAT
PRIMER_INTERNAL_2_SEQUENCE=AGGCTTGTGGCAATTGGTTA
PRIMER_LEFT_2=40,20
PRIMER_RIGHT_2=123,20
PRIMER_INTERNAL_2=82,20
PRIMER_LEFT_2_TM=59.654
PRIMER_RIGHT_2_TM=59.934
PRIMER_INTERNAL_2_TM=60.502
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=84
PRIMER_PAIR_3_PENALTY=0.488010
PRIMER_LEFT_3_PENALTY=0.356870
PRIMER_RIGHT_3_PENALTY=0.131140
PRIMER_INTERNAL_3_PENALTY=3.964821
PRIMER_LEFT_3_SEQUENCE=TTTCGCTGGGAAAGTTTGAG
PRIMER_RIGHT_3_SEQUENCE=CGGTTTTCAGTGGCTAGGAG
PRIMER_INTERNAL_3_SEQUENCE=AAGGGGAGTTGATGTGTACAA
PRIMER_LEFT_3=135,20
PRIMER_RIGHT_3=223,20
PRIMER_INTERNAL_3=159,21
PRIMER_LEFT_3_TM=60.357
PRIMER_RIGHT_3_TM=59.869
PRIMER_INTERNAL_3_TM=57.035
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=89
PRIMER_PAIR_4_PENALTY=0.561912
PRIMER_LEFT_4_PENALTY=0.234379
PRIMER_RIGHT_4_PENALTY=0.327533
PRIMER_INTERNAL_4_PENALTY=0.501869
PRIMER_LEFT_4_SEQUENCE=TTTTCGTGATTCTTGTGGCA
PRIMER_RIGHT_4_SEQUENCE=AATACACAGGAGGGAAGGGC
PRIMER_INTERNAL_4_SEQUENCE=AGGCTTGTGGCAATTGGTTA
PRIMER_LEFT_4=37,20
PRIMER_RIGHT_4=135,20
PRIMER_INTERNAL_4=82,20
PRIMER_LEFT_4_TM=60.234
PRIMER_RIGHT_4_TM=60.328
PRIMER_INTERNAL_4_TM=60.502
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH976
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGCCCCTCTGTGTTGCTTTAGCCAAAGGTTAAGTTTACCTCCTCCAGGAGCCTTCTCTGATAACCTCAGCTGACACTGATATCCCCCATCAACTGACTGAAGGCAAGGATCTTGTCTTATACCACTTTGTAGTTTCCTAGAGTCTTACATTTTGAATTTAGTGATAACTGAAAAACTACCCCTTATTTCTTAAACTATTACACAGGTAATTACATAGAGTTTAAAAGTTTCTTAAATACATGTCATTTAAAAAGTAGTTGTTCATGAAGTGTTAAG
SEQUENCE_INCLUDED_REGION=36,280
P3_COMMENT=CL is CAGCTGACACTGATATCCCC
P3_COMMENT=CR is TCATGAACAACTACTTTTTAAATGA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.450612
PRIMER_LEFT_0_PENALTY=0.124655
PRIMER_RIGHT_0_PENALTY=0.325957
PRIMER_INTERNAL_0_PENALTY=0.073866
PRIMER_LEFT_0_SEQUENCE=CCCTCTGTGTTGCTTTAGCC
PRIMER_RIGHT_0_SEQUENCE=TCAGTCAGTTGATGGGGGAT
PRIMER_INTERNAL_0_SEQUENCE=GTTTACCTCCTCCAGGAGCC
PRIMER_LEFT_0=43,20
PRIMER_RIGHT_0=139,20
PRIMER_INTERNAL_0=72,20
PRIMER_LEFT_0_TM=59.875
PRIMER_RIGHT_0_TM=60.326
PRIMER_INTERNAL_0_TM=60.074
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=8.7000
PRIMER_RIGHT_0_END_STABILITY=9.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=97
PRIMER_PAIR_1_PENALTY=0.450612
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.325957
PRIMER_INTERNAL_1_PENALTY=0.073866
PRIMER_LEFT_1_SEQUENCE=CCCCTCTGTGTTGCTTTAGC
PRIMER_RIGHT_1_SEQUENCE=TCAGTCAGTTGATGGGGGAT
PRIMER_INTERNAL_1_SEQUENCE=GTTTACCTCCTCCAGGAGCC
PRIMER_LEFT_1=42,20
PRIMER_RIGHT_1=139,20
PRIMER_INTERNAL_1=72,20
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.326
PRIMER_INTERNAL_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=98
PRIMER_PAIR_2_PENALTY=0.450612
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.325957
PRIMER_INTERNAL_2_PENALTY=0.073866
PRIMER_LEFT_2_SEQUENCE=GCCCCTCTGTGTTGCTTTAG
PRIMER_RIGHT_2_SEQUENCE=TCAGTCAGTTGATGGGGGAT
PRIMER_INTERNAL_2_SEQUENCE=GTTTACCTCCTCCAGGAGCC
PRIMER_LEFT_2=41,20
PRIMER_RIGHT_2=139,20
PRIMER_INTERNAL_2=72,20
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=60.326
PRIMER_INTERNAL_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=99
PRIMER_PAIR_3_PENALTY=0.450612
PRIMER_LEFT_3_PENALTY=0.124655
PRIMER_RIGHT_3_PENALTY=0.325957
PRIMER_INTERNAL_3_PENALTY=0.073866
PRIMER_LEFT_3_SEQUENCE=AGCCCCTCTGTGTTGCTTTA
PRIMER_RIGHT_3_SEQUENCE=TCAGTCAGTTGATGGGGGAT
PRIMER_INTERNAL_3_SEQUENCE=GTTTACCTCCTCCAGGAGCC
PRIMER_LEFT_3=40,20
PRIMER_RIGHT_3=139,20
PRIMER_INTERNAL_3=72,20
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=60.326
PRIMER_INTERNAL_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=100
PRIMER_PAIR_4_PENALTY=0.625483
PRIMER_LEFT_4_PENALTY=0.124655
PRIMER_RIGHT_4_PENALTY=0.500828
PRIMER_INTERNAL_4_PENALTY=0.073866
PRIMER_LEFT_4_SEQUENCE=CCCTCTGTGTTGCTTTAGCC
PRIMER_RIGHT_4_SEQUENCE=CTTCAGTCAGTTGATGGGGG
PRIMER_INTERNAL_4_SEQUENCE=GTTTACCTCCTCCAGGAGCC
PRIMER_LEFT_4=43,20
PRIMER_RIGHT_4=141,20
PRIMER_INTERNAL_4=72,20
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=60.501
PRIMER_INTERNAL_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
SEQUENCE_ID=MH977
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATAAGTACAGTTCAGCCTTTTCTCCTCAAATATATAATGACTTTAACATTCCTAAGAATATAGGTATTTCTGAATGATTTAAATTTGAGGAATTTTAATACATAAAATACAATGTACAAACTTTCTGCCCACTCAGATCTCTTCTCCATCATGTACTTAGTATTTCCCATTAACCTACACACTGATTTTTATGCTACTCCTTGTAGAAACAAAATTCTGGTTTGACTCAGTTTTTGTGTTTATAAACTTTTGGAATGTGTACCCCGTTTATGTGAAGAATTATGACCTATCAGTCATAG
SEQUENCE_INCLUDED_REGION=36,302
P3_COMMENT=CL is CTGATAAGTACAGTTCAGCCTTT
P3_COMMENT=CR is ATGACTGATAGGTCATAATTCTTCA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=6.481595
PRIMER_LEFT_0_PENALTY=2.032347
PRIMER_RIGHT_0_PENALTY=4.449248
PRIMER_INTERNAL_0_PENALTY=5.526775
PRIMER_LEFT_0_SEQUENCE=CAATGTACAAACTTTCTGCCCA
PRIMER_RIGHT_0_SEQUENCE=TCAGTGTGTAGGTTAATGGGAAA
PRIMER_INTERNAL_0_SEQUENCE=CTCAGATCTCTTCTCCATCATGT
PRIMER_LEFT_0=148,22
PRIMER_RIGHT_0=223,23
PRIMER_INTERNAL_0=170,23
PRIMER_LEFT_0_TM=60.032
PRIMER_RIGHT_0_TM=58.551
PRIMER_INTERNAL_0_TM=57.473
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_INTERNAL_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=11.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=76
PRIMER_PAIR_1_PENALTY=6.809408
PRIMER_LEFT_1_PENALTY=6.720996
PRIMER_RIGHT_1_PENALTY=0.088413
PRIMER_INTERNAL_1_PENALTY=3.685690
PRIMER_LEFT_1_SEQUENCE=CACTGATTTTTATGCTACTCCTTG
PRIMER_RIGHT_1_SEQUENCE=ACGGGGTACACATTCCAAAA
PRIMER_INTERNAL_1_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG
PRIMER_LEFT_1=218,24
PRIMER_RIGHT_1=305,20
PRIMER_INTERNAL_1=254,23
PRIMER_LEFT_1_TM=57.279
PRIMER_RIGHT_1_TM=60.088
PRIMER_INTERNAL_1_TM=59.314
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=88
PRIMER_PAIR_2_PENALTY=6.809408
PRIMER_LEFT_2_PENALTY=6.720996
PRIMER_RIGHT_2_PENALTY=0.088413
PRIMER_INTERNAL_2_PENALTY=3.685690
PRIMER_LEFT_2_SEQUENCE=CACTGATTTTTATGCTACTCCTTG
PRIMER_RIGHT_2_SEQUENCE=AACGGGGTACACATTCCAAA
PRIMER_INTERNAL_2_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG
PRIMER_LEFT_2=218,24
PRIMER_RIGHT_2=306,20
PRIMER_INTERNAL_2=254,23
PRIMER_LEFT_2_TM=57.279
PRIMER_RIGHT_2_TM=60.088
PRIMER_INTERNAL_2_TM=59.314
PRIMER_LEFT_2_GC_PERCENT=37.500
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=89
PRIMER_PAIR_3_PENALTY=6.809408
PRIMER_LEFT_3_PENALTY=6.720996
PRIMER_RIGHT_3_PENALTY=0.088413
PRIMER_INTERNAL_3_PENALTY=3.685690
PRIMER_LEFT_3_SEQUENCE=CACTGATTTTTATGCTACTCCTTG
PRIMER_RIGHT_3_SEQUENCE=AAACGGGGTACACATTCCAA
PRIMER_INTERNAL_3_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG
PRIMER_LEFT_3=218,24
PRIMER_RIGHT_3=307,20
PRIMER_INTERNAL_3=254,23
PRIMER_LEFT_3_TM=57.279
PRIMER_RIGHT_3_TM=60.088
PRIMER_INTERNAL_3_TM=59.314
PRIMER_LEFT_3_GC_PERCENT=37.500
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=90
PRIMER_PAIR_4_PENALTY=6.842323
PRIMER_LEFT_4_PENALTY=1.351236
PRIMER_RIGHT_4_PENALTY=5.491087
PRIMER_INTERNAL_4_PENALTY=4.449248
PRIMER_LEFT_4_SEQUENCE=GCCCACTCAGATCTCTTCTCC
PRIMER_RIGHT_4_SEQUENCE=CCAGAATTTTGTTTCTACAAGGA
PRIMER_INTERNAL_4_SEQUENCE=TTTCCCATTAACCTACACACTGA
PRIMER_LEFT_4=165,21
PRIMER_RIGHT_4=258,23
PRIMER_INTERNAL_4=201,23
PRIMER_LEFT_4_TM=60.351
PRIMER_RIGHT_4_TM=57.509
PRIMER_INTERNAL_4_TM=58.551
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_INTERNAL_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=94
=
SEQUENCE_ID=MH978
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGAGAGAAGTCAAAGCCTGGCTTCAAAGCTTCAAAGGACAGGCTCATTCCCTTGTTAGGGGCTAATGCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
SEQUENCE_INCLUDED_REGION=36,71
P3_COMMENT=CL is 
P3_COMMENT=CR is 
P3_COMMENT=CM is _
P3_COMMENT=CC is 0,_
PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
SEQUENCE_ID=MH979
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGTACTTGTGATGAATGTATTTGTTCTCATTGGTTCCAGAGCATTTAAAAGTGTAAGTTGATATCAGGATTTCCTTCACCTTTTGGTCACATACTTTGCCAACTGTTAGACATTTGCGTATTTTAAATCCTACAGTTTTAGCGTGTCTTGGGCCTGTCATCTTTGCTAGTACACGTTGCTGTACTTGTCTGTTAATCCCTAGATCTTCAAAGTACAAAGAATGGTTCTAATAGGTATTGTTATCACATATAAGAAAGAACATCACATAGAAGTTTAGAGTTGTAAGGACTCTAAGGATGATCTAGCCCAAACCC
SEQUENCE_INCLUDED_REGION=36,315
P3_COMMENT=CL is GATGAATGTATTTGTTCTCATTGG
P3_COMMENT=CR is GGTTTGGGCTAGATCATCCT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.011171
PRIMER_LEFT_0_PENALTY=0.891362
PRIMER_RIGHT_0_PENALTY=0.119809
PRIMER_INTERNAL_0_PENALTY=1.683262
PRIMER_LEFT_0_SEQUENCE=TCCTTCACCTTTTGGTCACA
PRIMER_RIGHT_0_SEQUENCE=AGATGACAGGCCCAAGACAC
PRIMER_INTERNAL_0_SEQUENCE=GCCAACTGTTAGACATTTGCG
PRIMER_LEFT_0=108,20
PRIMER_RIGHT_0=198,20
PRIMER_INTERNAL_0=134,21
PRIMER_LEFT_0_TM=59.109
PRIMER_RIGHT_0_TM=60.120
PRIMER_INTERNAL_0_TM=60.683
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=6.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=91
PRIMER_PAIR_1_PENALTY=1.055020
PRIMER_LEFT_1_PENALTY=0.891362
PRIMER_RIGHT_1_PENALTY=0.163658
PRIMER_INTERNAL_1_PENALTY=1.683262
PRIMER_LEFT_1_SEQUENCE=TCCTTCACCTTTTGGTCACA
PRIMER_RIGHT_1_SEQUENCE=CTAGCAAAGATGACAGGCCC
PRIMER_INTERNAL_1_SEQUENCE=GCCAACTGTTAGACATTTGCG
PRIMER_LEFT_1=108,20
PRIMER_RIGHT_1=205,20
PRIMER_INTERNAL_1=134,21
PRIMER_LEFT_1_TM=59.109
PRIMER_RIGHT_1_TM=59.836
PRIMER_INTERNAL_1_TM=60.683
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=98
PRIMER_PAIR_2_PENALTY=1.508756
PRIMER_LEFT_2_PENALTY=0.891362
PRIMER_RIGHT_2_PENALTY=0.617395
PRIMER_INTERNAL_2_PENALTY=1.683262
PRIMER_LEFT_2_SEQUENCE=TCCTTCACCTTTTGGTCACA
PRIMER_RIGHT_2_SEQUENCE=GCCCAAGACACGCTAAAACT
PRIMER_INTERNAL_2_SEQUENCE=GCCAACTGTTAGACATTTGCG
PRIMER_LEFT_2=108,20
PRIMER_RIGHT_2=189,20
PRIMER_INTERNAL_2=134,21
PRIMER_LEFT_2_TM=59.109
PRIMER_RIGHT_2_TM=59.383
PRIMER_INTERNAL_2_TM=60.683
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=82
PRIMER_PAIR_3_PENALTY=1.547754
PRIMER_LEFT_3_PENALTY=0.891362
PRIMER_RIGHT_3_PENALTY=0.656393
PRIMER_INTERNAL_3_PENALTY=1.683262
PRIMER_LEFT_3_SEQUENCE=TCCTTCACCTTTTGGTCACA
PRIMER_RIGHT_3_SEQUENCE=AAGATGACAGGCCCAAGACA
PRIMER_INTERNAL_3_SEQUENCE=GCCAACTGTTAGACATTTGCG
PRIMER_LEFT_3=108,20
PRIMER_RIGHT_3=199,20
PRIMER_INTERNAL_3=134,21
PRIMER_LEFT_3_TM=59.109
PRIMER_RIGHT_3_TM=60.656
PRIMER_INTERNAL_3_TM=60.683
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=1.633444
PRIMER_LEFT_4_PENALTY=1.513635
PRIMER_RIGHT_4_PENALTY=0.119809
PRIMER_INTERNAL_4_PENALTY=1.683262
PRIMER_LEFT_4_SEQUENCE=TTCCTTCACCTTTTGGTCACA
PRIMER_RIGHT_4_SEQUENCE=AGATGACAGGCCCAAGACAC
PRIMER_INTERNAL_4_SEQUENCE=GCCAACTGTTAGACATTTGCG
PRIMER_LEFT_4=107,21
PRIMER_RIGHT_4=198,20
PRIMER_INTERNAL_4=134,21
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=60.120
PRIMER_INTERNAL_4_TM=60.683
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=6.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=92
=
SEQUENCE_ID=MH982
SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGACCTTTTAAGTGAACTATCTGGCACTGTTTGAAATTGCATCAGCCAGTTGGAATAAGAATTCGCTCTCCTGTGAGACTATTCTTATACAGCATCATGTTATGTGTGTAATAAAGTCATATTTGGCCTTTGCCCCCATGTCACATCACAGAGCCCCATTCCTGATAAGAGTGTCCTTTGTTATTCATAGCGAGCCCCTGTTAACCATACCTAAGTTTGTTCTAATGTGGTAACTCACTGTAGGCACCTGGGTAG
SEQUENCE_INCLUDED_REGION=36,257
P3_COMMENT=CL is ACTGTTTGAAATTGCATCAGC
P3_COMMENT=CR is CCAGGTGCCTACAGTGAGTT
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.233598
PRIMER_LEFT_0_PENALTY=0.059762
PRIMER_RIGHT_0_PENALTY=0.173836
PRIMER_INTERNAL_0_PENALTY=6.660950
PRIMER_LEFT_0_SEQUENCE=TGTTATTCATAGCGAGCCCC
PRIMER_RIGHT_0_SEQUENCE=ACCCAGGTGCCTACAGTGAG
PRIMER_INTERNAL_0_SEQUENCE=CCATACCTAAGTTTGTTCTAATGTGG
PRIMER_LEFT_0=216,20
PRIMER_RIGHT_0=290,20
PRIMER_INTERNAL_0=242,26
PRIMER_LEFT_0_TM=60.060
PRIMER_RIGHT_0_TM=60.174
PRIMER_INTERNAL_0_TM=59.339
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_INTERNAL_0_GC_PERCENT=38.462
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=12.4000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=75
PRIMER_PAIR_1_PENALTY=0.593264
PRIMER_LEFT_1_PENALTY=0.045756
PRIMER_RIGHT_1_PENALTY=0.547508
PRIMER_INTERNAL_1_PENALTY=2.173354
PRIMER_LEFT_1_SEQUENCE=CCCCATGTCACATCACAGAG
PRIMER_RIGHT_1_SEQUENCE=GTATGGTTAACAGGGGCTCG
PRIMER_INTERNAL_1_SEQUENCE=CCCATTCCTGATAAGAGTGTCC
PRIMER_LEFT_1=171,20
PRIMER_RIGHT_1=247,20
PRIMER_INTERNAL_1=192,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=59.452
PRIMER_INTERNAL_1_TM=59.827
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=0.668902
PRIMER_LEFT_2_PENALTY=0.121394
PRIMER_RIGHT_2_PENALTY=0.547508
PRIMER_INTERNAL_2_PENALTY=4.099075
PRIMER_LEFT_2_SEQUENCE=CCATGTCACATCACAGAGCC
PRIMER_RIGHT_2_SEQUENCE=GTATGGTTAACAGGGGCTCG
PRIMER_INTERNAL_2_SEQUENCE=CCATTCCTGATAAGAGTGTCCTTT
PRIMER_LEFT_2=173,20
PRIMER_RIGHT_2=247,20
PRIMER_INTERNAL_2=193,24
PRIMER_LEFT_2_TM=60.121
PRIMER_RIGHT_2_TM=59.452
PRIMER_INTERNAL_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PAIR_2_PRODUCT_SIZE=75
PRIMER_PAIR_3_PENALTY=0.668902
PRIMER_LEFT_3_PENALTY=0.121394
PRIMER_RIGHT_3_PENALTY=0.547508
PRIMER_INTERNAL_3_PENALTY=2.173354
PRIMER_LEFT_3_SEQUENCE=CCCATGTCACATCACAGAGC
PRIMER_RIGHT_3_SEQUENCE=GTATGGTTAACAGGGGCTCG
PRIMER_INTERNAL_3_SEQUENCE=CCCATTCCTGATAAGAGTGTCC
PRIMER_LEFT_3=172,20
PRIMER_RIGHT_3=247,20
PRIMER_INTERNAL_3=192,22
PRIMER_LEFT_3_TM=60.121
PRIMER_RIGHT_3_TM=59.452
PRIMER_INTERNAL_3_TM=59.827
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=76
PRIMER_PAIR_4_PENALTY=0.898714
PRIMER_LEFT_4_PENALTY=0.059762
PRIMER_RIGHT_4_PENALTY=0.838952
PRIMER_INTERNAL_4_PENALTY=6.660950
PRIMER_LEFT_4_SEQUENCE=TGTTATTCATAGCGAGCCCC
PRIMER_RIGHT_4_SEQUENCE=TACCCAGGTGCCTACAGTGA
PRIMER_INTERNAL_4_SEQUENCE=CCATACCTAAGTTTGTTCTAATGTGG
PRIMER_LEFT_4=216,20
PRIMER_RIGHT_4=291,20
PRIMER_INTERNAL_4=242,26
PRIMER_LEFT_4_TM=60.060
PRIMER_RIGHT_4_TM=59.161
PRIMER_INTERNAL_4_TM=59.339
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=38.462
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=76
=
SEQUENCE_ID=MH986
SEQUENCE_TEMPLATE=CTTCATGCTCCAGGTCGACTCTAGAGGATCCCTGCTGTCTGGGCCCGGCAAATTGGTTTAAGTGGAATGAAATTGGTTCCTTCCCTCCCCGTGCTCATGGCTGAGGTCCAGGCCCGATTGTAATGGGAACCTCTCCTGGGCAAAGGACATAGAAAAGCTTTCCGGGGAGTCTGACCCTCTTTTTTCATATGTGAATTCTACTTNCCCCTGGGGGTCAAGGCAATAGGCTGATAAAACATANAAATGAACCTGAAGTGGGTGGGGTGAGTGTGTGTGTGGGAAGTTGGATCAGAGGAGAATGAACAATGAACAGTGGCCGAGTGTTTTTCAAGGGCCAGTTAACCATTCCCCTGTTG
SEQUENCE_INCLUDED_REGION=32,324
P3_COMMENT=CL is GGCAAATTGGTTTAAGTGGA
P3_COMMENT=CR is ACAGGGGAATGGTTAACTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.289767
PRIMER_LEFT_0_PENALTY=0.255861
PRIMER_RIGHT_0_PENALTY=0.033905
PRIMER_INTERNAL_0_PENALTY=0.755541
PRIMER_LEFT_0_SEQUENCE=GAAATTGGTTCCTTCCCTCC
PRIMER_RIGHT_0_SEQUENCE=TCCCCGGAAAGCTTTTCTAT
PRIMER_INTERNAL_0_SEQUENCE=CCGATTGTAATGGGAACCTC
PRIMER_LEFT_0=68,20
PRIMER_RIGHT_0=167,20
PRIMER_INTERNAL_0=113,20
PRIMER_LEFT_0_TM=59.744
PRIMER_RIGHT_0_TM=60.034
PRIMER_INTERNAL_0_TM=59.244
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=5.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_1_PENALTY=0.297318
PRIMER_LEFT_1_PENALTY=0.110369
PRIMER_RIGHT_1_PENALTY=0.186949
PRIMER_INTERNAL_1_PENALTY=0.156287
PRIMER_LEFT_1_SEQUENCE=GTGTGTGTGTGGGAAGTTGG
PRIMER_RIGHT_1_SEQUENCE=GGAATGGTTAACTGGCCCTT
PRIMER_INTERNAL_1_SEQUENCE=ACAGTGGCCGAGTGTTTTTC
PRIMER_LEFT_1=267,20
PRIMER_RIGHT_1=348,20
PRIMER_INTERNAL_1=309,20
PRIMER_LEFT_1_TM=59.890
PRIMER_RIGHT_1_TM=60.187
PRIMER_INTERNAL_1_TM=60.156
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=82
PRIMER_PAIR_2_PENALTY=0.322184
PRIMER_LEFT_2_PENALTY=0.255861
PRIMER_RIGHT_2_PENALTY=0.066323
PRIMER_INTERNAL_2_PENALTY=0.755541
PRIMER_LEFT_2_SEQUENCE=GAAATTGGTTCCTTCCCTCC
PRIMER_RIGHT_2_SEQUENCE=TTCTATGTCCTTTGCCCAGG
PRIMER_INTERNAL_2_SEQUENCE=CCGATTGTAATGGGAACCTC
PRIMER_LEFT_2=68,20
PRIMER_RIGHT_2=153,20
PRIMER_INTERNAL_2=113,20
PRIMER_LEFT_2_TM=59.744
PRIMER_RIGHT_2_TM=60.066
PRIMER_INTERNAL_2_TM=59.244
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=86
PRIMER_PAIR_3_PENALTY=0.335962
PRIMER_LEFT_3_PENALTY=0.179674
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_INTERNAL_3_PENALTY=0.110369
PRIMER_LEFT_3_SEQUENCE=AATGAACCTGAAGTGGGTGG
PRIMER_RIGHT_3_SEQUENCE=AAAACACTCGGCCACTGTTC
PRIMER_INTERNAL_3_SEQUENCE=GTGTGTGTGTGGGAAGTTGG
PRIMER_LEFT_3=242,20
PRIMER_RIGHT_3=326,20
PRIMER_INTERNAL_3=267,20
PRIMER_LEFT_3_TM=59.820
PRIMER_RIGHT_3_TM=60.156
PRIMER_INTERNAL_3_TM=59.890
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=85
PRIMER_PAIR_4_PENALTY=0.335962
PRIMER_LEFT_4_PENALTY=0.179674
PRIMER_RIGHT_4_PENALTY=0.156287
PRIMER_INTERNAL_4_PENALTY=0.110369
PRIMER_LEFT_4_SEQUENCE=AATGAACCTGAAGTGGGTGG
PRIMER_RIGHT_4_SEQUENCE=GAAAAACACTCGGCCACTGT
PRIMER_INTERNAL_4_SEQUENCE=GTGTGTGTGTGGGAAGTTGG
PRIMER_LEFT_4=242,20
PRIMER_RIGHT_4=328,20
PRIMER_INTERNAL_4=267,20
PRIMER_LEFT_4_TM=59.820
PRIMER_RIGHT_4_TM=60.156
PRIMER_INTERNAL_4_TM=59.890
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=6.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH989
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATAATTTTCCCACTGAGTATGGCTTTCAGTAGTTTTATTATGATGTGCCTAGGTACATTTGTTTTTATTTGTTCTGCGAATTGTTGTATTACTTTGGGAGAAATGCTCAACTATAAATATTGCTTCTGACCCTTTTCTGTGTTCCTTCTTAAAGATACAAAATAAATGTAACATTAGACCTCTCACTATGCTGTTTTTACTCTCCTCTGATTTTTTTTTCCATTATTTTTATTGCTCTGGCTTCATTTTGTAAATNTGCTTCTTGAAATTTTTCTTTTAATTCTAGAATATCTATTTGGTTCTTTTCATGGTTTCTAGTTCTCTGAAAATG
SEQUENCE_INCLUDED_REGION=33,336
P3_COMMENT=CL is TCCCACTGAGTATGGCTTTC
P3_COMMENT=CR is CTAGAAACCATGAAAAGAACCA
P3_COMMENT=CM is TTTTATTATGATGTGCCTAGGTACA
P3_COMMENT=CC is 1,1
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=4.462135
PRIMER_LEFT_0_PENALTY=1.257221
PRIMER_RIGHT_0_PENALTY=3.204914
PRIMER_INTERNAL_0_PENALTY=4.166289
PRIMER_LEFT_0_SEQUENCE=CCCACTGAGTATGGCTTTCAG
PRIMER_RIGHT_0_SEQUENCE=CCCAAAGTAATACAACAATTCGC
PRIMER_INTERNAL_0_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
PRIMER_LEFT_0=46,21
PRIMER_RIGHT_0=135,23
PRIMER_INTERNAL_0=78,24
PRIMER_LEFT_0_TM=59.743
PRIMER_RIGHT_0_TM=59.795
PRIMER_INTERNAL_0_TM=59.834
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_INTERNAL_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=12.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=10.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_1_PENALTY=4.483080
PRIMER_LEFT_1_PENALTY=1.278166
PRIMER_RIGHT_1_PENALTY=3.204914
PRIMER_INTERNAL_1_PENALTY=4.166289
PRIMER_LEFT_1_SEQUENCE=CCCACTGAGTATGGCTTTCA
PRIMER_RIGHT_1_SEQUENCE=CCCAAAGTAATACAACAATTCGC
PRIMER_INTERNAL_1_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
PRIMER_LEFT_1=46,20
PRIMER_RIGHT_1=135,23
PRIMER_INTERNAL_1=78,24
PRIMER_LEFT_1_TM=58.722
PRIMER_RIGHT_1_TM=59.795
PRIMER_INTERNAL_1_TM=59.834
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=12.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=90
PRIMER_PAIR_2_PENALTY=4.846658
PRIMER_LEFT_2_PENALTY=1.641744
PRIMER_RIGHT_2_PENALTY=3.204914
PRIMER_INTERNAL_2_PENALTY=4.166289
PRIMER_LEFT_2_SEQUENCE=TCCCACTGAGTATGGCTTTCA
PRIMER_RIGHT_2_SEQUENCE=CCCAAAGTAATACAACAATTCGC
PRIMER_INTERNAL_2_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
PRIMER_LEFT_2=45,21
PRIMER_RIGHT_2=135,23
PRIMER_INTERNAL_2=78,24
PRIMER_LEFT_2_TM=60.642
PRIMER_RIGHT_2_TM=59.795
PRIMER_INTERNAL_2_TM=59.834
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=12.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=91
PRIMER_PAIR_3_PENALTY=4.969665
PRIMER_LEFT_3_PENALTY=1.257221
PRIMER_RIGHT_3_PENALTY=3.712444
PRIMER_INTERNAL_3_PENALTY=4.166289
PRIMER_LEFT_3_SEQUENCE=CCCACTGAGTATGGCTTTCAG
PRIMER_RIGHT_3_SEQUENCE=ACAACAATTCGCAGAACAAAT
PRIMER_INTERNAL_3_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
PRIMER_LEFT_3=46,21
PRIMER_RIGHT_3=124,21
PRIMER_INTERNAL_3=78,24
PRIMER_LEFT_3_TM=59.743
PRIMER_RIGHT_3_TM=57.288
PRIMER_INTERNAL_3_TM=59.834
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=33.333
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=12.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=4.990610
PRIMER_LEFT_4_PENALTY=1.278166
PRIMER_RIGHT_4_PENALTY=3.712444
PRIMER_INTERNAL_4_PENALTY=4.166289
PRIMER_LEFT_4_SEQUENCE=CCCACTGAGTATGGCTTTCA
PRIMER_RIGHT_4_SEQUENCE=ACAACAATTCGCAGAACAAAT
PRIMER_INTERNAL_4_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT
PRIMER_LEFT_4=46,20
PRIMER_RIGHT_4=124,21
PRIMER_INTERNAL_4=78,24
PRIMER_LEFT_4_TM=58.722
PRIMER_RIGHT_4_TM=57.288
PRIMER_INTERNAL_4_TM=59.834
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=33.333
PRIMER_INTERNAL_4_GC_PERCENT=37.500
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=12.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=79
=
SEQUENCE_ID=MH991
SEQUENCE_TEMPLATE=CTTCTGCAGGACATTTGTACCATCGAGGTCAAAGGCAACAAGAAGTGACATGAGTTTCAGTCCATCTTCATGAGGTTCCAGTTAAGGCCTGTGAATTCGCAATTGTTCTTCCCGACTGCCTGTCCTGTGAACCTCACATACTCACATCAGATGCAAAAACAACCACCTTGTAGAAG
SEQUENCE_INCLUDED_REGION=0,176
P3_COMMENT=CL is GAGGTCAAAGGCAACAAGAA
P3_COMMENT=CR is AAGGTGGTTGTTTTTGCATC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.731126
PRIMER_LEFT_0_PENALTY=0.444466
PRIMER_RIGHT_0_PENALTY=0.286660
PRIMER_INTERNAL_0_PENALTY=0.991035
PRIMER_LEFT_0_SEQUENCE=GTACCATCGAGGTCAAAGGC
PRIMER_RIGHT_0_SEQUENCE=GCGAATTCACAGGCCTTAAC
PRIMER_INTERNAL_0_SEQUENCE=TCCATCTTCATGAGGTTCCA
PRIMER_LEFT_0=16,20
PRIMER_RIGHT_0=99,20
PRIMER_INTERNAL_0=60,20
PRIMER_LEFT_0_TM=59.556
PRIMER_RIGHT_0_TM=59.713
PRIMER_INTERNAL_0_TM=59.009
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_INTERNAL_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=4.00
PRIMER_PAIR_0_PRODUCT_SIZE=84
PRIMER_PAIR_1_PENALTY=0.852407
PRIMER_LEFT_1_PENALTY=0.444466
PRIMER_RIGHT_1_PENALTY=0.407941
PRIMER_INTERNAL_1_PENALTY=3.274264
PRIMER_LEFT_1_SEQUENCE=GTACCATCGAGGTCAAAGGC
PRIMER_RIGHT_1_SEQUENCE=CACAGGCCTTAACTGGAACC
PRIMER_INTERNAL_1_SEQUENCE=TGAGTTTCAGTCCATCTTCATGA
PRIMER_LEFT_1=16,20
PRIMER_RIGHT_1=92,20
PRIMER_INTERNAL_1=50,23
PRIMER_LEFT_1_TM=59.556
PRIMER_RIGHT_1_TM=59.592
PRIMER_INTERNAL_1_TM=59.726
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_INTERNAL_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_INTERNAL_1_SELF_END=6.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=77
PRIMER_PAIR_2_PENALTY=0.922693
PRIMER_LEFT_2_PENALTY=0.636033
PRIMER_RIGHT_2_PENALTY=0.286660
PRIMER_INTERNAL_2_PENALTY=0.991035
PRIMER_LEFT_2_SEQUENCE=ATCGAGGTCAAAGGCAACAA
PRIMER_RIGHT_2_SEQUENCE=GCGAATTCACAGGCCTTAAC
PRIMER_INTERNAL_2_SEQUENCE=TCCATCTTCATGAGGTTCCA
PRIMER_LEFT_2=21,20
PRIMER_RIGHT_2=99,20
PRIMER_INTERNAL_2=60,20
PRIMER_LEFT_2_TM=60.636
PRIMER_RIGHT_2_TM=59.713
PRIMER_INTERNAL_2_TM=59.009
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_INTERNAL_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=1.214573
PRIMER_LEFT_3_PENALTY=0.286660
PRIMER_RIGHT_3_PENALTY=0.927913
PRIMER_INTERNAL_3_PENALTY=0.243525
PRIMER_LEFT_3_SEQUENCE=GTTAAGGCCTGTGAATTCGC
PRIMER_RIGHT_3_SEQUENCE=ACAAGGTGGTTGTTTTTGCA
PRIMER_INTERNAL_3_SEQUENCE=ACTGCCTGTCCTGTGAACCT
PRIMER_LEFT_3=80,20
PRIMER_RIGHT_3=170,20
PRIMER_INTERNAL_3=114,20
PRIMER_LEFT_3_TM=59.713
PRIMER_RIGHT_3_TM=59.072
PRIMER_INTERNAL_3_TM=59.756
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=91
PRIMER_PAIR_4_PENALTY=1.262277
PRIMER_LEFT_4_PENALTY=0.286660
PRIMER_RIGHT_4_PENALTY=0.975617
PRIMER_INTERNAL_4_PENALTY=0.243525
PRIMER_LEFT_4_SEQUENCE=GTTAAGGCCTGTGAATTCGC
PRIMER_RIGHT_4_SEQUENCE=GGTGGTTGTTTTTGCATCTG
PRIMER_INTERNAL_4_SEQUENCE=ACTGCCTGTCCTGTGAACCT
PRIMER_LEFT_4=80,20
PRIMER_RIGHT_4=166,20
PRIMER_INTERNAL_4=114,20
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=59.024
PRIMER_INTERNAL_4_TM=59.756
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_INTERNAL_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=87
=
SEQUENCE_ID=MH993
SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAACCTTTTGTTTCTTTCTTTTTCAGTACACTGCTTTAATAAATGGTAACAGTGCTTTAATAAGTCCCCTGGCCATCATCTTACTTATGATCTCTAGATGAAGACCACTGTGTGAAACTGGTATCCTAGGAGGTTGGAGTGAGCATGTATGAGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGCTTGATTTATAAACCACTGAAGCCCGATGAAGTACTAGGGTGAAAATCACCTACGTTTCCAGAGGAAAAAAATGTCACCATCAATTCCAAGAGGGACTTGTAAACCTGAAACTAACAGCCAATGGTTTAATTTTTTTAAGGTAGAT
SEQUENCE_INCLUDED_REGION=33,342
P3_COMMENT=CL is TTTCTTTCTTTTTCAGTACACTGC
P3_COMMENT=CR is ACCTTAAAAAAATTAAACCATTGG
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.666116
PRIMER_LEFT_0_PENALTY=0.546307
PRIMER_RIGHT_0_PENALTY=0.119809
PRIMER_INTERNAL_0_PENALTY=2.335679
PRIMER_LEFT_0_SEQUENCE=ATAAGTCCCCTGGCCATCAT
PRIMER_RIGHT_0_SEQUENCE=ACATGCTCACTCCAACCTCC
PRIMER_INTERNAL_0_SEQUENCE=GAAGACCACTGTGTGAAACTGG
PRIMER_LEFT_0=92,20
PRIMER_RIGHT_0=180,20
PRIMER_INTERNAL_0=132,22
PRIMER_LEFT_0_TM=60.546
PRIMER_RIGHT_0_TM=60.120
PRIMER_INTERNAL_0_TM=59.664
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.5000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_1_PENALTY=0.713625
PRIMER_LEFT_1_PENALTY=0.593816
PRIMER_RIGHT_1_PENALTY=0.119809
PRIMER_INTERNAL_1_PENALTY=2.335679
PRIMER_LEFT_1_SEQUENCE=TTTAATAAGTCCCCTGGCCA
PRIMER_RIGHT_1_SEQUENCE=ACATGCTCACTCCAACCTCC
PRIMER_INTERNAL_1_SEQUENCE=GAAGACCACTGTGTGAAACTGG
PRIMER_LEFT_1=88,20
PRIMER_RIGHT_1=180,20
PRIMER_INTERNAL_1=132,22
PRIMER_LEFT_1_TM=59.406
PRIMER_RIGHT_1_TM=60.120
PRIMER_INTERNAL_1_TM=59.664
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=93
PRIMER_PAIR_2_PENALTY=0.807063
PRIMER_LEFT_2_PENALTY=0.546307
PRIMER_RIGHT_2_PENALTY=0.260757
PRIMER_INTERNAL_2_PENALTY=2.335679
PRIMER_LEFT_2_SEQUENCE=ATAAGTCCCCTGGCCATCAT
PRIMER_RIGHT_2_SEQUENCE=CATGCTCACTCCAACCTCCT
PRIMER_INTERNAL_2_SEQUENCE=GAAGACCACTGTGTGAAACTGG
PRIMER_LEFT_2=92,20
PRIMER_RIGHT_2=179,20
PRIMER_INTERNAL_2=132,22
PRIMER_LEFT_2_TM=60.546
PRIMER_RIGHT_2_TM=60.261
PRIMER_INTERNAL_2_TM=59.664
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_INTERNAL_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=88
PRIMER_PAIR_3_PENALTY=0.854573
PRIMER_LEFT_3_PENALTY=0.593816
PRIMER_RIGHT_3_PENALTY=0.260757
PRIMER_INTERNAL_3_PENALTY=2.335679
PRIMER_LEFT_3_SEQUENCE=TTTAATAAGTCCCCTGGCCA
PRIMER_RIGHT_3_SEQUENCE=CATGCTCACTCCAACCTCCT
PRIMER_INTERNAL_3_SEQUENCE=GAAGACCACTGTGTGAAACTGG
PRIMER_LEFT_3=88,20
PRIMER_RIGHT_3=179,20
PRIMER_INTERNAL_3=132,22
PRIMER_LEFT_3_TM=59.406
PRIMER_RIGHT_3_TM=60.261
PRIMER_INTERNAL_3_TM=59.664
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=92
PRIMER_PAIR_4_PENALTY=1.113647
PRIMER_LEFT_4_PENALTY=0.993838
PRIMER_RIGHT_4_PENALTY=0.119809
PRIMER_INTERNAL_4_PENALTY=2.335679
PRIMER_LEFT_4_SEQUENCE=CCCTGGCCATCATCTTACTT
PRIMER_RIGHT_4_SEQUENCE=ACATGCTCACTCCAACCTCC
PRIMER_INTERNAL_4_SEQUENCE=GAAGACCACTGTGTGAAACTGG
PRIMER_LEFT_4=99,20
PRIMER_RIGHT_4=180,20
PRIMER_INTERNAL_4=132,22
PRIMER_LEFT_4_TM=59.006
PRIMER_RIGHT_4_TM=60.120
PRIMER_INTERNAL_4_TM=59.664
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=82
=
SEQUENCE_ID=MH996
SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAAGTAAGACATCTAAATTAGCAGATTTCATTCTTCCAAAATTAGAAAGTCATTAAGTTACAGATTTAGTTATGGAAGGATGGATGATGGCAGGTAAGTACTTAGGACTAAGTAGGAGCCACTCCTTAAGGAATCAATGTGCTTTTCAAATGTGTCATCTCATTTTATCCTGAACAACAGAAAATATTTTCTAGGTAAAGAAATTGAGGCTTAAAGAAGTAAAGTTATCTATAANCTAATAGTTTATAGACTCATTCCTGGTCTCTCACTAGATAATTTGCTGCGTGCTCAATTTTAGTCATGCAGGGAGTACTTACTAAACTTTACTTGGTATGCATATAATCAT
SEQUENCE_INCLUDED_REGION=36,351
P3_COMMENT=CL is AAGACATCTAAATTAGCAGATTTCA
P3_COMMENT=CR is CCTGCATGACTAAAATTGAGC
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.335435
PRIMER_LEFT_0_PENALTY=0.303491
PRIMER_RIGHT_0_PENALTY=3.031944
PRIMER_INTERNAL_0_PENALTY=1.998311
PRIMER_LEFT_0_SEQUENCE=AGGATGGATGATGGCAGGTA
PRIMER_RIGHT_0_SEQUENCE=GACACATTTGAAAAGCACATTGA
PRIMER_INTERNAL_0_SEQUENCE=AGGAGCCACTCCTTAAGGAA
PRIMER_LEFT_0=117,20
PRIMER_RIGHT_0=196,23
PRIMER_INTERNAL_0=154,20
PRIMER_LEFT_0_TM=60.303
PRIMER_RIGHT_0_TM=60.032
PRIMER_INTERNAL_0_TM=58.002
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=34.783
PRIMER_INTERNAL_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_INTERNAL_0_SELF_ANY=10.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=8.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=80
PRIMER_PAIR_1_PENALTY=3.626232
PRIMER_LEFT_1_PENALTY=0.303491
PRIMER_RIGHT_1_PENALTY=3.322740
PRIMER_INTERNAL_1_PENALTY=1.815562
PRIMER_LEFT_1_SEQUENCE=AGGATGGATGATGGCAGGTA
PRIMER_RIGHT_1_SEQUENCE=GATGACACATTTGAAAAGCACA
PRIMER_INTERNAL_1_SEQUENCE=GGAGCCACTCCTTAAGGAATC
PRIMER_LEFT_1=117,20
PRIMER_RIGHT_1=199,22
PRIMER_INTERNAL_1=155,21
PRIMER_LEFT_1_TM=60.303
PRIMER_RIGHT_1_TM=58.677
PRIMER_INTERNAL_1_TM=59.184
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=36.364
PRIMER_INTERNAL_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_INTERNAL_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=8.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=83
PRIMER_PAIR_2_PENALTY=3.635253
PRIMER_LEFT_2_PENALTY=0.303491
PRIMER_RIGHT_2_PENALTY=3.331761
PRIMER_INTERNAL_2_PENALTY=1.815562
PRIMER_LEFT_2_SEQUENCE=AGGATGGATGATGGCAGGTA
PRIMER_RIGHT_2_SEQUENCE=AGATGACACATTTGAAAAGCACA
PRIMER_INTERNAL_2_SEQUENCE=GGAGCCACTCCTTAAGGAATC
PRIMER_LEFT_2=117,20
PRIMER_RIGHT_2=200,23
PRIMER_INTERNAL_2=155,21
PRIMER_LEFT_2_TM=60.303
PRIMER_RIGHT_2_TM=59.668
PRIMER_INTERNAL_2_TM=59.184
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_INTERNAL_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=10.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_INTERNAL_2_SELF_END=8.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=84
PRIMER_PAIR_3_PENALTY=3.722889
PRIMER_LEFT_3_PENALTY=0.690945
PRIMER_RIGHT_3_PENALTY=3.031944
PRIMER_INTERNAL_3_PENALTY=1.998311
PRIMER_LEFT_3_SEQUENCE=GGATGGATGATGGCAGGTAA
PRIMER_RIGHT_3_SEQUENCE=GACACATTTGAAAAGCACATTGA
PRIMER_INTERNAL_3_SEQUENCE=AGGAGCCACTCCTTAAGGAA
PRIMER_LEFT_3=118,20
PRIMER_RIGHT_3=196,23
PRIMER_INTERNAL_3=154,20
PRIMER_LEFT_3_TM=60.691
PRIMER_RIGHT_3_TM=60.032
PRIMER_INTERNAL_3_TM=58.002
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=10.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_INTERNAL_3_SELF_END=8.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=79
PRIMER_PAIR_4_PENALTY=3.966652
PRIMER_LEFT_4_PENALTY=0.303491
PRIMER_RIGHT_4_PENALTY=3.663161
PRIMER_INTERNAL_4_PENALTY=1.815562
PRIMER_LEFT_4_SEQUENCE=AGGATGGATGATGGCAGGTA
PRIMER_RIGHT_4_SEQUENCE=TGAGATGACACATTTGAAAAGCA
PRIMER_INTERNAL_4_SEQUENCE=GGAGCCACTCCTTAAGGAATC
PRIMER_LEFT_4=117,20
PRIMER_RIGHT_4=202,23
PRIMER_INTERNAL_4=155,21
PRIMER_LEFT_4_TM=60.303
PRIMER_RIGHT_4_TM=60.663
PRIMER_INTERNAL_4_TM=59.184
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_INTERNAL_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_INTERNAL_4_SELF_ANY=10.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_INTERNAL_4_SELF_END=8.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=86
=
SEQUENCE_ID=MH997
SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTATTTCTCTGGGCACTGCTTTCTTTGGGGGCAAACTTCCAGTATCACTGATACTAATATAAAAACCCTGTAAGTCTGCTTGCATTTTCAAGATTCAATATATATCCAGATTGTTTTCCCAGCAAAGAAAATTTTATTTCTCAAGATATAAAAAATAAATATTTAATTTCAGTTTCCTCAAAAGGAATATGAAATTTGTTAAAATGCAAATCCAGCTGTAACTTTTTTGGACTTGTCTTTTATTTCTTGCTTAGTAAAAGCACTATTAGTTTGGCATCATCAAATGAGTAGGGATGAG
SEQUENCE_INCLUDED_REGION=35,301
P3_COMMENT=CL is CTCTTATTTCTCTGGGCACTG
P3_COMMENT=CR is TCATCCCTACTCATTTGATGA
P3_COMMENT=CM is _
P3_COMMENT=CC is 1,_
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=1.068237
PRIMER_LEFT_0_PENALTY=0.067436
PRIMER_RIGHT_0_PENALTY=1.000801
PRIMER_INTERNAL_0_PENALTY=1.071885
PRIMER_LEFT_0_SEQUENCE=GGGGCAAACTTCCAGTATCA
PRIMER_RIGHT_0_SEQUENCE=TTGCTGGGAAAACAATCTGG
PRIMER_INTERNAL_0_SEQUENCE=AACCCTGTAAGTCTGCTTGCA
PRIMER_LEFT_0=65,20
PRIMER_RIGHT_0=162,20
PRIMER_INTERNAL_0=101,21
PRIMER_LEFT_0_TM=59.933
PRIMER_RIGHT_0_TM=61.001
PRIMER_INTERNAL_0_TM=59.928
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_INTERNAL_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=5.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=98
PRIMER_PAIR_1_PENALTY=1.083074
PRIMER_LEFT_1_PENALTY=0.067436
PRIMER_RIGHT_1_PENALTY=1.015638
PRIMER_INTERNAL_1_PENALTY=1.071885
PRIMER_LEFT_1_SEQUENCE=GGGGCAAACTTCCAGTATCA
PRIMER_RIGHT_1_SEQUENCE=GCTGGGAAAACAATCTGGAT
PRIMER_INTERNAL_1_SEQUENCE=AACCCTGTAAGTCTGCTTGCA
PRIMER_LEFT_1=65,20
PRIMER_RIGHT_1=160,20
PRIMER_INTERNAL_1=101,21
PRIMER_LEFT_1_TM=59.933
PRIMER_RIGHT_1_TM=58.984
PRIMER_INTERNAL_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_INTERNAL_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_INTERNAL_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_INTERNAL_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=96
PRIMER_PAIR_2_PENALTY=1.205626
PRIMER_LEFT_2_PENALTY=0.133741
PRIMER_RIGHT_2_PENALTY=1.071885
PRIMER_INTERNAL_2_PENALTY=0.067436
PRIMER_LEFT_2_SEQUENCE=TCTCTGGGCACTGCTTTCTT
PRIMER_RIGHT_2_SEQUENCE=TGCAAGCAGACTTACAGGGTT
PRIMER_INTERNAL_2_SEQUENCE=GGGGCAAACTTCCAGTATCA
PRIMER_LEFT_2=43,20
PRIMER_RIGHT_2=121,21
PRIMER_INTERNAL_2=65,20
PRIMER_LEFT_2_TM=60.134
PRIMER_RIGHT_2_TM=59.928
PRIMER_INTERNAL_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_INTERNAL_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=79
PRIMER_PAIR_3_PENALTY=1.205626
PRIMER_LEFT_3_PENALTY=0.133741
PRIMER_RIGHT_3_PENALTY=1.071885
PRIMER_INTERNAL_3_PENALTY=0.067436
PRIMER_LEFT_3_SEQUENCE=TTCTCTGGGCACTGCTTTCT
PRIMER_RIGHT_3_SEQUENCE=TGCAAGCAGACTTACAGGGTT
PRIMER_INTERNAL_3_SEQUENCE=GGGGCAAACTTCCAGTATCA
PRIMER_LEFT_3=42,20
PRIMER_RIGHT_3=121,21
PRIMER_INTERNAL_3=65,20
PRIMER_LEFT_3_TM=60.134
PRIMER_RIGHT_3_TM=59.928
PRIMER_INTERNAL_3_TM=59.933
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_INTERNAL_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=80
PRIMER_PAIR_4_PENALTY=1.322358
PRIMER_LEFT_4_PENALTY=0.067436
PRIMER_RIGHT_4_PENALTY=1.254922
PRIMER_INTERNAL_4_PENALTY=1.071885
PRIMER_LEFT_4_SEQUENCE=GGGGCAAACTTCCAGTATCA
PRIMER_RIGHT_4_SEQUENCE=TTTGCTGGGAAAACAATCTG
PRIMER_INTERNAL_4_SEQUENCE=AACCCTGTAAGTCTGCTTGCA
PRIMER_LEFT_4=65,20
PRIMER_RIGHT_4=163,20
PRIMER_INTERNAL_4=101,21
PRIMER_LEFT_4_TM=59.933
PRIMER_RIGHT_4_TM=58.745
PRIMER_INTERNAL_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_INTERNAL_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=5.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=99
=
