PRIMER_TM_SANTALUCIA=0
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=0
PRIMER_NUM_RETURN=1
PRIMER_TASK=pick_left_only
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_SIZE=10
PRIMER_MAX_SIZE=10
PRIMER_OPT_SIZE=10
PRIMER_MIN_TM=0
PRIMER_MAX_TM=70
COMMENT=nomasking_Breslauer_Schildkraut
PRIMER_SEQUENCE_ID=000
SEQUENCE=TCCTTGTGAT
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=44.8937
PRIMER_LEFT_SEQUENCE=TCCTTGTGAT
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=15.106
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
=
PRIMER_TM_SANTALUCIA=0
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=0
COMMENT=nomasking_Breslauer_SantaLucia
PRIMER_SEQUENCE_ID=001
SEQUENCE=TCCTTGTGAT
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=37.1275
PRIMER_LEFT_SEQUENCE=TCCTTGTGAT
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=22.873
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
=
PRIMER_TM_SANTALUCIA=0
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
COMMENT=nomasking_Breslauer_Owczarzy
PRIMER_SEQUENCE_ID=002
SEQUENCE=TCCTTGTGAT
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=37.1758
PRIMER_LEFT_SEQUENCE=TCCTTGTGAT
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=22.824
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
=
PRIMER_TM_SANTALUCIA=0
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=1
COMMENT=masking_Breslauer_Schildkraut
COMMENT=Should fail
PRIMER_SEQUENCE_ID=100
SEQUENCE=TCCTTGTGAt
PRIMER_LEFT_EXPLAIN=considered 1,lowercase masking of 3' end 1, ok 0
=
PRIMER_TM_SANTALUCIA=0
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=1
PRIMER_SEQUENCE_ID=101
COMMENT=masking_Breslauer_SantaLucia
COMMENT=Should succeed
SEQUENCE=TCCTTGTGaT
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=37.1275
PRIMER_LEFT_SEQUENCE=TCCTTGTGaT
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=22.873
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
=
PRIMER_TM_SANTALUCIA=0
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
COMMENT=masking_Breslauer_Owczarzy
COMMENT=Should succeed
PRIMER_SEQUENCE_ID=102
SEQUENCE=tccttgtgat
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=37.1758
PRIMER_LEFT_SEQUENCE=tccttgtgat
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=22.824
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
=
PRIMER_TM_SANTALUCIA=1
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=0
COMMENT=nomasking_SantaLucia_Schildkraut
PRIMER_SEQUENCE_ID=010
SEQUENCE=TCCTTGTGAT
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=50.2651
PRIMER_LEFT_SEQUENCE=TCCTTGTGAT
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=9.735
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=3.0600
=
PRIMER_TM_SANTALUCIA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=0
COMMENT=nomasking_SantaLucia_SantaLucia
PRIMER_SEQUENCE_ID=011
SEQUENCE=TCCTTGTGAG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=39.0572
PRIMER_LEFT_SEQUENCE=TCCTTGTGAG
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=20.943
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=3.5100
=
PRIMER_TM_SANTALUCIA=1
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
COMMENT=nomasking_SantaLucia_Owczarzy
PRIMER_SEQUENCE_ID=012
SEQUENCE=GCCTTGTGAG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=33.9227
PRIMER_LEFT_SEQUENCE=GCCTTGTGAG
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=26.077
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=3.5100
=
PRIMER_TM_SANTALUCIA=1
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=1
COMMENT=masking_SantaLucia_Schildkraut
COMMENT=Should fail
PRIMER_SEQUENCE_ID=110
SEQUENCE=TCCTTGTGAt
PRIMER_LEFT_EXPLAIN=considered 1,lowercase masking of 3' end 1, ok 0
=
PRIMER_TM_SANTALUCIA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=1
COMMENT=masking_SantaLucia_SantaLucia
COMMENT=Should succeed
PRIMER_SEQUENCE_ID=111
SEQUENCE=TCCTTGTGaT
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=41.6015
PRIMER_LEFT_SEQUENCE=TCCTTGTGaT
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=18.398
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=3.0600
=
PRIMER_TM_SANTALUCIA=1
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
COMMENT=nomasking_SantaLucia_Owczarzy
COMMENT=Should succeed
PRIMER_SEQUENCE_ID=112
SEQUENCE=tccttgtgat
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=42.0803
PRIMER_LEFT_SEQUENCE=tccttgtgat
PRIMER_LEFT=0,10
PRIMER_LEFT_TM=17.920
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=3.0600
=
