PRIMER_SEQUENCE_ID=anyway_1
PRIMER_EXPLAIN_FLAG=1
PRIMER_SELF_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
PRIMER_PICK_ANYWAY=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=high_tm
PRIMER_SELF_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
PRIMER_WARNING=Left primer is unacceptable: Tm too high
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_target
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=20,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
PRIMER_WARNING=Left primer is unacceptable: Overlaps Target/Tm too high/High self complementarity
PRIMER_RIGHT_EXPLAIN=considered 3, high tm 2, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_excl_region
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
EXCLUDED_REGION=20,1
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
PRIMER_WARNING=Left primer is unacceptable: Overlaps Excluded Region/Tm too high/High self complementarity
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_incl_region
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
INCLUDED_REGION=1,160
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_MAX_TM=60
PRIMER_ERROR=Specified left primer not in Included Region
=
PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_MAX_TM=60
PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Unacceptable GC content
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=internal_too_short
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccat
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
PRIMER_ERROR=Specified internal oligo too short
=
PRIMER_SEQUENCE_ID=internal_ok
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_INTERNAL_OLIGO=23,19
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_INTERNAL_OLIGO_TM=59.420
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=6.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=internal_too_cold
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122 143,1
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_INTERNAL_OLIGO_MIN_TM=60
PRIMER_MAX_TM=60
PRIMER_MAX_GC=60
PRIMER_WARNING=Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content; Hybridization probe is unacceptable: Tm too low
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_INTERNAL_OLIGO=23,19
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_INTERNAL_OLIGO_TM=59.420
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=6.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
=
PRIMER_SEQUENCE_ID=internal_too_cold2
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=12
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_INTERNAL_OLIGO_MIN_TM=60
PRIMER_MAX_TM=100
PRIMER_MAX_GC=100
PRIMER_WARNING=Hybridization probe is unacceptable: Tm too low
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=4.0864
PRIMER_LEFT_PENALTY=1.771733
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
PRIMER_LEFT_SEQUENCE=CCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
PRIMER_LEFT=1,20
PRIMER_RIGHT=160,18
PRIMER_INTERNAL_OLIGO=23,19
PRIMER_LEFT_TM=58.228
PRIMER_RIGHT_TM=59.685
PRIMER_INTERNAL_OLIGO_TM=59.420
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=10.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=6.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=160
=
PRIMER_SEQUENCE_ID=mispriming
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_SELF_ANY=11
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=12
PRIMER_NUM_RETURN=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG
PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc
PRIMER_INTERNAL_OLIGO_MIN_TM=60
PRIMER_MAX_TM=100
PRIMER_MAX_GC=100
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_MAX_MISPRIMING=8
PRIMER_WARNING=Left primer is unacceptable: High similarity to mispriming or mishyb library; Hybridization probe is unacceptable: Tm too low
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=4.0864
PRIMER_LEFT_PENALTY=1.771733
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_INTERNAL_OLIGO_PENALTY=1.580164
PRIMER_LEFT_SEQUENCE=CCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_INTERNAL_OLIGO_SEQUENCE=gcgtacgatccatgctagc
PRIMER_LEFT=1,20
PRIMER_RIGHT=160,18
PRIMER_INTERNAL_OLIGO=23,19
PRIMER_LEFT_TM=58.228
PRIMER_RIGHT_TM=59.685
PRIMER_INTERNAL_OLIGO_TM=59.420
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_INTERNAL_OLIGO_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=10.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=6.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=160
=
