PRIMER_SEQUENCE_ID=00
PRIMER_MAX_POLY_X=0
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=0
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=50-100
PRIMER_MIN_TM=0.0
PRIMER_MAX_TM=100.0
PRIMER_MIN_SIZE=25
PRIMER_MAX_SIZE=25
PRIMER_INTERNAL_OLIGO_MIN_SIZE=25
PRIMER_INTERNAL_OLIGO_MAX_SIZE=25
PRIMER_INTERNAL_OLIGO_OPT_SIZE=25
PRIMER_OPT_SIZE=25
PRIMER_MAX_GC=100
PRIMER_MIN_GC=0
PRIMER_SELF_END=100
PRIMER_SELF_ANY=100
PRIMER_INTERNAL_OLIGO_SELF_ANY=100
PRIMER_INTERNAL_OLIGO_SELF_END=100 
PRIMER_INTERNAL_OLIGO_MAX_TM=100
PRIMER_INTERNAL_OLIGO_MIN_TM=30
PRIMER_INTERNAL_OLIGO_MAX_GC=100
PRIMER_INTERNAL_OLIGO_MIN_GC=0
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
PRIMER_SEQUENCE_ID=01
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
PRIMER_SEQUENCE_ID=02
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_PAIR_EXPLAIN=considered 2, ok 2
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
PRIMER_PAIR_PENALTY_1=14.2271
PRIMER_LEFT_1_PENALTY=2.731673
PRIMER_RIGHT_1_PENALTY=11.495378
PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_1_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
PRIMER_LEFT_1=2,25
PRIMER_RIGHT_1=54,25
PRIMER_LEFT_1_TM=62.732
PRIMER_RIGHT_1_TM=71.495
PRIMER_LEFT_1_GC_PERCENT=36.000
PRIMER_RIGHT_1_GC_PERCENT=56.000
PRIMER_LEFT_1_SELF_ANY=12.00
PRIMER_RIGHT_1_SELF_ANY=12.00
PRIMER_LEFT_1_SELF_END=10.00
PRIMER_RIGHT_1_SELF_END=12.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=53
=
PRIMER_SEQUENCE_ID=03
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
PRIMER_SEQUENCE_ID=04
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
PRIMER_SEQUENCE_ID=05
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
PRIMER_SEQUENCE_ID=06
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
PRIMER_SEQUENCE_ID=07
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1
PRIMER_PAIR_PENALTY=14.2271
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
PRIMER_LEFT=2,25
PRIMER_RIGHT=54,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=12.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=53
=
PRIMER_SEQUENCE_ID=08
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=14.2271
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT
PRIMER_LEFT=2,25
PRIMER_RIGHT=54,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=12.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=53
=
PRIMER_SEQUENCE_ID=09
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, no internal oligo 1, ok 0
=
PRIMER_SEQUENCE_ID=10
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0
=
PRIMER_SEQUENCE_ID=11
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAATTTGATTG
PRIMER_RIGHT_INPUT=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_ERROR=Specified left primer too short; Specified left primer not in sequence
=
PRIMER_SEQUENCE_ID=12
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_INPUT=TGCGTCGACTGCAACGTACGTACGT
PRIMER_INTERNAL_OLIGO_INPUT=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_ERROR=Not specified to pick internal oligos but a specific internal oligo is provided
=
PRIMER_SEQUENCE_ID=13
SEQUENCE=nncacgtcaTCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=cacgtcatctcatcaaatttgattg
PRIMER_RIGHT_INPUT=gaattgtttggaaatgacagtgagt
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=cacgtcaTCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
PRIMER_SEQUENCE_ID=14
SEQUENCE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
PRIMER_LEFT_INPUT=CACGTCATctcatcaaattTGATTG
PRIMER_RIGHT_INPUT=gaattgtttggaAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 60, too many Ns 57, ok 3
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.3813
PRIMER_LEFT_PENALTY=2.731673
PRIMER_RIGHT_PENALTY=0.649600
PRIMER_INTERNAL_OLIGO_PENALTY=11.495378
PRIMER_LEFT_SEQUENCE=CACGTCATCTCATCAAATTTGATTG
PRIMER_RIGHT_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_INTERNAL_OLIGO_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_LEFT=2,25
PRIMER_RIGHT=81,25
PRIMER_INTERNAL_OLIGO=30,25
PRIMER_LEFT_TM=62.732
PRIMER_RIGHT_TM=60.650
PRIMER_INTERNAL_OLIGO_TM=71.495
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=36.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=56.000
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_LEFT_SELF_END=10.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=8.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=80
=
