PRIMER_FIRST_BASE_INDEX=1
PRIMER_SELF_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_FILE_FLAG=1
PRIMER_SEQUENCE_ID=targets_test
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=22,122
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=1,21
PRIMER_RIGHT=161,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=4.0864
PRIMER_LEFT_1_PENALTY=1.771733
PRIMER_RIGHT_1_PENALTY=2.314641
PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_1=2,20
PRIMER_RIGHT_1=161,18
PRIMER_LEFT_1_TM=58.228
PRIMER_RIGHT_1_TM=59.685
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=5.0463
PRIMER_LEFT_2_PENALTY=2.731698
PRIMER_RIGHT_2_PENALTY=2.314641
PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_2=1,20
PRIMER_RIGHT_2=161,18
PRIMER_LEFT_2_TM=57.268
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=161
=
PRIMER_SEQUENCE_ID=SP01-02.txt
SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
PRIMER_PAIR_PENALTY=2.3342
PRIMER_LEFT_PENALTY=2.101896
PRIMER_RIGHT_PENALTY=0.232299
PRIMER_LEFT_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_LEFT=10,22
PRIMER_RIGHT=248,20
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.232
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=2.7735
PRIMER_LEFT_1_PENALTY=2.101896
PRIMER_RIGHT_1_PENALTY=0.671597
PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=247,20
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.328
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=2.8468
PRIMER_LEFT_2_PENALTY=2.101896
PRIMER_RIGHT_2_PENALTY=0.744925
PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=249,20
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.745
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=3.0984
PRIMER_LEFT_3_PENALTY=2.866131
PRIMER_RIGHT_3_PENALTY=0.232299
PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_LEFT_3=12,21
PRIMER_RIGHT_3=248,20
PRIMER_LEFT_3_TM=58.134
PRIMER_RIGHT_3_TM=60.232
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=3.2356
PRIMER_LEFT_4_PENALTY=3.003259
PRIMER_RIGHT_4_PENALTY=0.232299
PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=248,20
PRIMER_LEFT_4_TM=58.997
PRIMER_RIGHT_4_TM=60.232
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SP01-03.txt
SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_PAIR_PENALTY=0.7850
PRIMER_LEFT_PENALTY=0.040772
PRIMER_RIGHT_PENALTY=0.744257
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_RIGHT_SEQUENCE=ATCCTGCTGAAGCAAATGGA
PRIMER_LEFT=73,20
PRIMER_RIGHT=174,20
PRIMER_LEFT_TM=59.959
PRIMER_RIGHT_TM=60.744
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.7850
PRIMER_LEFT_1_PENALTY=0.040772
PRIMER_RIGHT_1_PENALTY=0.744257
PRIMER_LEFT_1_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_RIGHT_1_SEQUENCE=GATCCTGCTGAAGCAAATGG
PRIMER_LEFT_1=73,20
PRIMER_RIGHT_1=175,20
PRIMER_LEFT_1_TM=59.959
PRIMER_RIGHT_1_TM=60.744
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=103
PRIMER_PAIR_PENALTY_2=1.0159
PRIMER_LEFT_2_PENALTY=0.271680
PRIMER_RIGHT_2_PENALTY=0.744257
PRIMER_LEFT_2_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_RIGHT_2_SEQUENCE=ATCCTGCTGAAGCAAATGGA
PRIMER_LEFT_2=65,20
PRIMER_RIGHT_2=174,20
PRIMER_LEFT_2_TM=59.728
PRIMER_RIGHT_2_TM=60.744
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=1.0159
PRIMER_LEFT_3_PENALTY=0.271680
PRIMER_RIGHT_3_PENALTY=0.744257
PRIMER_LEFT_3_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_RIGHT_3_SEQUENCE=GATCCTGCTGAAGCAAATGG
PRIMER_LEFT_3=65,20
PRIMER_RIGHT_3=175,20
PRIMER_LEFT_3_TM=59.728
PRIMER_RIGHT_3_TM=60.744
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=111
PRIMER_PAIR_PENALTY_4=1.0159
PRIMER_LEFT_4_PENALTY=0.271680
PRIMER_RIGHT_4_PENALTY=0.744257
PRIMER_LEFT_4_SEQUENCE=CACCCTGCAGGTTTAGAAGG
PRIMER_RIGHT_4_SEQUENCE=ATCCTGCTGAAGCAAATGGA
PRIMER_LEFT_4=61,20
PRIMER_RIGHT_4=174,20
PRIMER_LEFT_4_TM=59.728
PRIMER_RIGHT_4_TM=60.744
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SP01-06.txt
SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_PAIR_PENALTY=6.7384
PRIMER_LEFT_PENALTY=4.596160
PRIMER_RIGHT_PENALTY=2.142251
PRIMER_LEFT_SEQUENCE=TATGAATCGCAATCAAATTTTTTC
PRIMER_RIGHT_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT=31,24
PRIMER_RIGHT=130,18
PRIMER_LEFT_TM=59.404
PRIMER_RIGHT_TM=60.142
PRIMER_LEFT_GC_PERCENT=25.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=7.1484
PRIMER_LEFT_1_PENALTY=5.006141
PRIMER_RIGHT_1_PENALTY=2.142251
PRIMER_LEFT_1_SEQUENCE=TTATGAATCGCAATCAAATTTTTT
PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_1=30,24
PRIMER_RIGHT_1=130,18
PRIMER_LEFT_1_TM=58.994
PRIMER_RIGHT_1_TM=60.142
PRIMER_LEFT_1_GC_PERCENT=20.833
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=7.3441
PRIMER_LEFT_2_PENALTY=5.201804
PRIMER_RIGHT_2_PENALTY=2.142251
PRIMER_LEFT_2_SEQUENCE=TATGAATCGCAATCAAATTTTTTCT
PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_2=31,25
PRIMER_RIGHT_2=130,18
PRIMER_LEFT_2_TM=60.202
PRIMER_RIGHT_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=24.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=7.3469
PRIMER_LEFT_3_PENALTY=5.204623
PRIMER_RIGHT_3_PENALTY=2.142251
PRIMER_LEFT_3_SEQUENCE=TATGAATCGCAATCAAATTTTTT
PRIMER_RIGHT_3_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_3=31,23
PRIMER_RIGHT_3=130,18
PRIMER_LEFT_3_TM=57.795
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=21.739
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=7.3469
PRIMER_LEFT_4_PENALTY=5.204623
PRIMER_RIGHT_4_PENALTY=2.142251
PRIMER_LEFT_4_SEQUENCE=TTATGAATCGCAATCAAATTTTT
PRIMER_RIGHT_4_SEQUENCE=GAGCGTGCTTTGCGTTTT
PRIMER_LEFT_4=30,23
PRIMER_RIGHT_4=130,18
PRIMER_LEFT_4_TM=57.795
PRIMER_RIGHT_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=21.739
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SP01-15.txt
SEQUENCE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC
=
PRIMER_SEQUENCE_ID=SP01-19.txt
SEQUENCE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC
PRIMER_PAIR_PENALTY=0.2699
PRIMER_LEFT_PENALTY=0.072951
PRIMER_RIGHT_PENALTY=0.196903
PRIMER_LEFT_SEQUENCE=TATTCCTGTCCCTGTGCCTC
PRIMER_RIGHT_SEQUENCE=CCCTCATCCAGTGCTCTTTC
PRIMER_LEFT=49,20
PRIMER_RIGHT=184,20
PRIMER_LEFT_TM=60.073
PRIMER_RIGHT_TM=59.803
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.2699
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.196903
PRIMER_LEFT_1_SEQUENCE=TATTCCTGTCCCTGTGCCTC
PRIMER_RIGHT_1_SEQUENCE=TCCCTCATCCAGTGCTCTTT
PRIMER_LEFT_1=49,20
PRIMER_RIGHT_1=185,20
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.3034
PRIMER_LEFT_2_PENALTY=0.072951
PRIMER_RIGHT_2_PENALTY=0.230410
PRIMER_LEFT_2_SEQUENCE=TATTCCTGTCCCTGTGCCTC
PRIMER_RIGHT_2_SEQUENCE=CAGTGCTCTTTCTGCCAGTG
PRIMER_LEFT_2=49,20
PRIMER_RIGHT_2=176,20
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=59.770
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=128
PRIMER_PAIR_PENALTY_3=0.5938
PRIMER_LEFT_3_PENALTY=0.396944
PRIMER_RIGHT_3_PENALTY=0.196903
PRIMER_LEFT_3_SEQUENCE=GCCTCAGAAACCTGGCTACA
PRIMER_RIGHT_3_SEQUENCE=CCCTCATCCAGTGCTCTTTC
PRIMER_LEFT_3=64,20
PRIMER_RIGHT_3=184,20
PRIMER_LEFT_3_TM=60.397
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.5938
PRIMER_LEFT_4_PENALTY=0.396944
PRIMER_RIGHT_4_PENALTY=0.196903
PRIMER_LEFT_4_SEQUENCE=GCCTCAGAAACCTGGCTACA
PRIMER_RIGHT_4_SEQUENCE=TCCCTCATCCAGTGCTCTTT
PRIMER_LEFT_4=64,20
PRIMER_RIGHT_4=185,20
PRIMER_LEFT_4_TM=60.397
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SP01-20.txt
SEQUENCE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC
=
PRIMER_SEQUENCE_ID=SP01-21.txt
SEQUENCE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC
PRIMER_PAIR_PENALTY=5.1035
PRIMER_LEFT_PENALTY=2.224770
PRIMER_RIGHT_PENALTY=2.878720
PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
PRIMER_LEFT=1,20
PRIMER_RIGHT=165,22
PRIMER_LEFT_TM=57.775
PRIMER_RIGHT_TM=59.121
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=5.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=5.1035
PRIMER_LEFT_1_PENALTY=2.224770
PRIMER_RIGHT_1_PENALTY=2.878720
PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=57.775
PRIMER_RIGHT_1_TM=59.121
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=5.1035
PRIMER_LEFT_2_PENALTY=2.224770
PRIMER_RIGHT_2_PENALTY=2.878720
PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
PRIMER_LEFT_2=1,20
PRIMER_RIGHT_2=156,22
PRIMER_LEFT_2_TM=57.775
PRIMER_RIGHT_2_TM=59.121
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=5.2887
PRIMER_LEFT_3_PENALTY=2.224770
PRIMER_RIGHT_3_PENALTY=3.063978
PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=166,23
PRIMER_LEFT_3_TM=57.775
PRIMER_RIGHT_3_TM=60.064
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=5.2887
PRIMER_LEFT_4_PENALTY=2.224770
PRIMER_RIGHT_4_PENALTY=3.063978
PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=165,23
PRIMER_LEFT_4_TM=57.775
PRIMER_RIGHT_4_TM=60.064
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SP03-40.txt
SEQUENCE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC
PRIMER_ERROR=INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE
=
PRIMER_SEQUENCE_ID=SP04-07.txt
SEQUENCE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA
PRIMER_PAIR_PENALTY=0.1033
PRIMER_LEFT_PENALTY=0.051674
PRIMER_RIGHT_PENALTY=0.051674
PRIMER_LEFT_SEQUENCE=CCCTACATACAGCCAAGGGA
PRIMER_RIGHT_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT=22,20
PRIMER_RIGHT=282,20
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=4.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.1033
PRIMER_LEFT_1_PENALTY=0.051674
PRIMER_RIGHT_1_PENALTY=0.051674
PRIMER_LEFT_1_SEQUENCE=TCCCTACATACAGCCAAGGG
PRIMER_RIGHT_1_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT_1=21,20
PRIMER_RIGHT_1=282,20
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=59.948
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.1180
PRIMER_LEFT_2_PENALTY=0.066323
PRIMER_RIGHT_2_PENALTY=0.051674
PRIMER_LEFT_2_SEQUENCE=CTAAGAATGTGGCCAAGGGA
PRIMER_RIGHT_2_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT_2=123,20
PRIMER_RIGHT_2=282,20
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.1180
PRIMER_LEFT_3_PENALTY=0.051674
PRIMER_RIGHT_3_PENALTY=0.066323
PRIMER_LEFT_3_SEQUENCE=CCCTACATACAGCCAAGGGA
PRIMER_RIGHT_3_SEQUENCE=CTTGGCCACATTCTTAGGGA
PRIMER_LEFT_3=22,20
PRIMER_RIGHT_3=139,20
PRIMER_LEFT_3_TM=59.948
PRIMER_RIGHT_3_TM=60.066
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.1180
PRIMER_LEFT_4_PENALTY=0.066323
PRIMER_RIGHT_4_PENALTY=0.051674
PRIMER_LEFT_4_SEQUENCE=TCCCTAAGAATGTGGCCAAG
PRIMER_RIGHT_4_SEQUENCE=TTCTTTCCGTGGCTCTCCTA
PRIMER_LEFT_4=120,20
PRIMER_RIGHT_4=282,20
PRIMER_LEFT_4_TM=60.066
PRIMER_RIGHT_4_TM=59.948
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=AGAG
SEQUENCE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
=
PRIMER_SEQUENCE_ID=long_seq
SEQUENCE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg
PRIMER_PAIR_PENALTY=0.3615
PRIMER_LEFT_PENALTY=0.221791
PRIMER_RIGHT_PENALTY=0.139753
PRIMER_LEFT_SEQUENCE=gatcgatgcatgcatgctac
PRIMER_RIGHT_SEQUENCE=cccagctagctagcatcgtc
PRIMER_LEFT=446,20
PRIMER_RIGHT=677,20
PRIMER_LEFT_TM=60.222
PRIMER_RIGHT_TM=60.140
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=60.000
PRIMER_LEFT_SELF_ANY=10.00
PRIMER_RIGHT_SELF_ANY=10.00
PRIMER_LEFT_SELF_END=8.00
PRIMER_RIGHT_SELF_END=4.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=5.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.3980
PRIMER_LEFT_1_PENALTY=0.258273
PRIMER_RIGHT_1_PENALTY=0.139753
PRIMER_LEFT_1_SEQUENCE=gatgctagctaggcgatgct
PRIMER_RIGHT_1_SEQUENCE=cccagctagctagcatcgtc
PRIMER_LEFT_1=409,20
PRIMER_RIGHT_1=677,20
PRIMER_LEFT_1_TM=59.742
PRIMER_RIGHT_1_TM=60.140
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=10.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=12.00
PRIMER_PAIR_1_COMPL_END=7.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.4051
PRIMER_LEFT_2_PENALTY=0.120169
PRIMER_RIGHT_2_PENALTY=0.284927
PRIMER_LEFT_2_SEQUENCE=gcgatcgatgctagctagct
PRIMER_RIGHT_2_SEQUENCE=ctagctagatcgccgctacg
PRIMER_LEFT_2=373,20
PRIMER_RIGHT_2=596,20
PRIMER_LEFT_2_TM=59.880
PRIMER_RIGHT_2_TM=60.285
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=10.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=8.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=10.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.5001
PRIMER_LEFT_3_PENALTY=0.221791
PRIMER_RIGHT_3_PENALTY=0.278329
PRIMER_LEFT_3_SEQUENCE=gatcgatgcatgcatgctac
PRIMER_RIGHT_3_SEQUENCE=gtcagctagctagatcgccg
PRIMER_LEFT_3=446,20
PRIMER_RIGHT_3=601,20
PRIMER_LEFT_3_TM=60.222
PRIMER_RIGHT_3_TM=60.278
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=10.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=8.00
PRIMER_RIGHT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=13.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.5067
PRIMER_LEFT_4_PENALTY=0.221791
PRIMER_RIGHT_4_PENALTY=0.284927
PRIMER_LEFT_4_SEQUENCE=gatcgatgcatgcatgctac
PRIMER_RIGHT_4_SEQUENCE=ctagctagatcgccgctacg
PRIMER_LEFT_4=446,20
PRIMER_RIGHT_4=596,20
PRIMER_LEFT_4_TM=60.222
PRIMER_RIGHT_4_TM=60.285
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=10.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=8.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=bad_letter
SEQUENCE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=bad_target
SEQUENCE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC
TARGET=51,10
TARGET=501,50
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=other_bad_target
SEQUENCE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC
TARGET=101,1000
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=included_test
SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
INCLUDED_REGION=21,228
PRIMER_PAIR_PENALTY=5.1035
PRIMER_LEFT_PENALTY=2.224770
PRIMER_RIGHT_PENALTY=2.878720
PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
PRIMER_LEFT=21,20
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=57.775
PRIMER_RIGHT_TM=59.121
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=5.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=5.1035
PRIMER_LEFT_1_PENALTY=2.224770
PRIMER_RIGHT_1_PENALTY=2.878720
PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
PRIMER_LEFT_1=21,20
PRIMER_RIGHT_1=187,22
PRIMER_LEFT_1_TM=57.775
PRIMER_RIGHT_1_TM=59.121
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=5.1035
PRIMER_LEFT_2_PENALTY=2.224770
PRIMER_RIGHT_2_PENALTY=2.878720
PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
PRIMER_LEFT_2=21,20
PRIMER_RIGHT_2=176,22
PRIMER_LEFT_2_TM=57.775
PRIMER_RIGHT_2_TM=59.121
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=5.2887
PRIMER_LEFT_3_PENALTY=2.224770
PRIMER_RIGHT_3_PENALTY=3.063978
PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
PRIMER_LEFT_3=21,20
PRIMER_RIGHT_3=186,23
PRIMER_LEFT_3_TM=57.775
PRIMER_RIGHT_3_TM=60.064
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=5.2887
PRIMER_LEFT_4_PENALTY=2.224770
PRIMER_RIGHT_4_PENALTY=3.063978
PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
PRIMER_LEFT_4=21,20
PRIMER_RIGHT_4=185,23
PRIMER_LEFT_4_TM=57.775
PRIMER_RIGHT_4_TM=60.064
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_OLIGO_SELF_END=8
PRIMER_SEQUENCE_ID=included_test_w_middle
SEQUENCE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG
INCLUDED_REGION=21,228
PRIMER_PAIR_PENALTY=5.1035
PRIMER_LEFT_PENALTY=2.224770
PRIMER_RIGHT_PENALTY=2.878720
PRIMER_INTERNAL_OLIGO_PENALTY=1.680010
PRIMER_LEFT_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_SEQUENCE=CAACAGACACACACACACAACA
PRIMER_INTERNAL_OLIGO_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT=21,20
PRIMER_RIGHT=185,22
PRIMER_INTERNAL_OLIGO=79,21
PRIMER_LEFT_TM=57.775
PRIMER_RIGHT_TM=59.121
PRIMER_INTERNAL_OLIGO_TM=60.680
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_INTERNAL_OLIGO_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=5.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=5.1035
PRIMER_LEFT_1_PENALTY=2.224770
PRIMER_RIGHT_1_PENALTY=2.878720
PRIMER_INTERNAL_OLIGO_1_PENALTY=1.680010
PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_1_SEQUENCE=CACAACAGACACACACACACAA
PRIMER_INTERNAL_OLIGO_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_1=21,20
PRIMER_RIGHT_1=187,22
PRIMER_INTERNAL_OLIGO_1=79,21
PRIMER_LEFT_1_TM=57.775
PRIMER_RIGHT_1_TM=59.121
PRIMER_INTERNAL_OLIGO_1_TM=60.680
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_INTERNAL_OLIGO_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=5.1035
PRIMER_LEFT_2_PENALTY=2.224770
PRIMER_RIGHT_2_PENALTY=2.878720
PRIMER_INTERNAL_OLIGO_2_PENALTY=1.680010
PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_2_SEQUENCE=CACACACACAACACACAGTCAA
PRIMER_INTERNAL_OLIGO_2_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_2=21,20
PRIMER_RIGHT_2=176,22
PRIMER_INTERNAL_OLIGO_2=79,21
PRIMER_LEFT_2_TM=57.775
PRIMER_RIGHT_2_TM=59.121
PRIMER_INTERNAL_OLIGO_2_TM=60.680
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_OLIGO_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=5.2887
PRIMER_LEFT_3_PENALTY=2.224770
PRIMER_RIGHT_3_PENALTY=3.063978
PRIMER_INTERNAL_OLIGO_3_PENALTY=1.680010
PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_3_SEQUENCE=ACAACAGACACACACACACAACA
PRIMER_INTERNAL_OLIGO_3_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_3=21,20
PRIMER_RIGHT_3=186,23
PRIMER_INTERNAL_OLIGO_3=79,21
PRIMER_LEFT_3_TM=57.775
PRIMER_RIGHT_3_TM=60.064
PRIMER_INTERNAL_OLIGO_3_TM=60.680
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_INTERNAL_OLIGO_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=5.2887
PRIMER_LEFT_4_PENALTY=2.224770
PRIMER_RIGHT_4_PENALTY=3.063978
PRIMER_INTERNAL_OLIGO_4_PENALTY=1.680010
PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT
PRIMER_RIGHT_4_SEQUENCE=CAACAGACACACACACACAACAC
PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG
PRIMER_LEFT_4=21,20
PRIMER_RIGHT_4=185,23
PRIMER_INTERNAL_OLIGO_4=79,21
PRIMER_LEFT_4_TM=57.775
PRIMER_RIGHT_4_TM=60.064
PRIMER_INTERNAL_OLIGO_4_TM=60.680
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_INTERNAL_OLIGO_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=6.00
PRIMER_PRODUCT_SIZE_4=165
=
