PRIMER_SEQUENCE_ID=template_mis_1
PRIMER_NUM_RETURN=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
=
PRIMER_SEQUENCE_ID=template_mis_2
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTG
=
PRIMER_SEQUENCE_ID=template_mis_3
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCC
=
PRIMER_SEQUENCE_ID=template_mis_4
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACSCCCCCCCCCCCCCCCCA
=
PRIMER_SEQUENCE_ID=template_mis_5
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
SEQUENCE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACTCCCCCCCCCCCCCCCCA
=
PRIMER_SEQUENCE_ID=template_mis_6
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCC
=
PRIMER_SEQUENCE_ID=template_mis_7
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATGat
PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
=
PRIMER_SEQUENCE_ID=template_mis_8
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=11
PRIMER_MAX_POLY_X=40
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_RIGHT_INPUT=GGGGGGGGGGGGGGGGGG
SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
COMMENT=Should succeed
=
PRIMER_SEQUENCE_ID=template_mis_9
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=9
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
COMMENT=Should succeed
=
PRIMER_SEQUENCE_ID=template_mis_10
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=6.7
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=1
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
SEQUENCE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
COMMENT=Should fail
=
PRIMER_SEQUENCE_ID=template_mis_11
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc
COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo
=
PRIMER_SEQUENCE_ID=template_mis_12
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=cTTGATGGAGAATTGCGCAGAGGGGc
INCLUDED_REGION=25,26
SEQUENCE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc
COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo plus former error in ignoring included_region offset
=
PRIMER_SEQUENCE_ID=template_mis_13
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_LEFT_INPUT=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE=cTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGc
COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo
=
PRIMER_SEQUENCE_ID=template_mis_14
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_RIGHT_INPUT=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE=GCCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAT
COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_11
=
PRIMER_SEQUENCE_ID=template_mis_15
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_RIGHT_INPUT=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG
COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13
=
PRIMER_SEQUENCE_ID=template_mis_16
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_RIGHT_INPUT=cTTGATGGAGAATTGCGCAGAGGGGc
INCLUDED_REGION=24,26
SEQUENCE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG
COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13
=
PRIMER_SEQUENCE_ID=short_align_target_1
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_COMPL_ANY=0.0
PRIMER_WT_COMPL_END=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_REP_SIM=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY=0.0
PRIMER_PAIR_WT_COMPL_END=0.0
PRIMER_PAIR_WT_REP_SIM=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
PRIMER_INTERNAL_OLIGO_OPT_SIZE=20
PRIMER_INTERNAL_OLIGO_MAX_SIZE=27
PRIMER_INTERNAL_OLIGO_MIN_TM=57.0
PRIMER_INTERNAL_OLIGO_OPT_TM=60.0
PRIMER_INTERNAL_OLIGO_MAX_TM=63.0
PRIMER_INTERNAL_OLIGO_MIN_GC=20.0
PRIMER_INTERNAL_OLIGO_MAX_GC=80.0
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_INTERNAL_OLIGO_SELF_END=12.00
PRIMER_INTERNAL_OLIGO_NUM_NS=0
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=5
PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=
PRIMER_INTERNAL_OLIGO_MAX_MISHYB=12.00
PRIMER_INTERNAL_OLIGO_MIN_QUALITY=0
PRIMER_INTERNAL_OLIGO_SALT_CONC=50.0
PRIMER_INTERNAL_OLIGO_DNA_CONC=50.0
PRIMER_INTERNAL_OLIGO_DIVALENT_CONC=0.0
PRIMER_INTERNAL_OLIGO_DNTP_CONC=0.0
PRIMER_IO_WT_TM_LT=1.0
PRIMER_IO_WT_TM_GT=1.0
PRIMER_IO_WT_SIZE_LT=1.0
PRIMER_IO_WT_SIZE_GT=1.0
PRIMER_IO_WT_GC_PERCENT_LT=0.0
PRIMER_IO_WT_GC_PERCENT_GT=0.0
PRIMER_IO_WT_COMPL_ANY=0.0
PRIMER_IO_WT_NUM_NS=0.0
PRIMER_IO_WT_REP_SIM=0.0
PRIMER_IO_WT_SEQ_QUAL=0.0
PRIMER_TASK=pick_right_only
PRIMER_FIRST_BASE_INDEX=1
PRIMER_PICK_ANYWAY=1
COMMENT=Test for very short target of alignment number 1
=
PRIMER_SEQUENCE_ID=short_align_target_2
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=
SEQUENCE=ACCAAGAAAGTAGTTGACAAAAAAATGAAAACCACTATTATTTAAATTACGGAAAAAAAAAAACCAATAAACAAAAAACATAATAAAAGAAATGAAAAAAAAGAATATTAGTAGAGCATACTGAATTGTTTTCAGAAAGAGAGTGAAAGGTAACAAAAATTTACCTACTTTTCCTTTTTATTTCTCTGGTTTCGTTTCTTTTTTTTCGGCCACCCTATTTATTTTTTCACTGCTTGTAAACAACGTCTCACTTCAATAGTGTCTCCTTCATGTACTGAGATACTGCTGCAAGCGAAATAT
PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000
PRIMER_NUM_RETURN=5
PRIMER_MAX_END_STABILITY=9.0
PRIMER_MAX_MISPRIMING=12.00
PRIMER_PAIR_MAX_MISPRIMING=24.00
PRIMER_MAX_TEMPLATE_MISPRIMING=12.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00
PRIMER_MIN_SIZE=18
PRIMER_OPT_SIZE=20
PRIMER_MAX_SIZE=27
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_MAX_TM=63.0
PRIMER_MAX_DIFF_TM=100.0
PRIMER_TM_SANTALUCIA=0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_SELF_ANY=8.00
PRIMER_SELF_END=3.00
PRIMER_NUM_NS_ACCEPTED=0
PRIMER_MAX_POLY_X=5
PRIMER_OUTSIDE_PENALTY=0
PRIMER_GC_CLAMP=0
PRIMER_SALT_CONC=50.0
PRIMER_SALT_CORRECTIONS=0
PRIMER_DIVALENT_CONC=0.0
PRIMER_DNTP_CONC=0.0
PRIMER_DNA_CONC=50.0
PRIMER_LIBERAL_BASE=1
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
PRIMER_MIN_QUALITY=0
PRIMER_MIN_END_QUALITY=0
PRIMER_QUALITY_RANGE_MIN=0
PRIMER_QUALITY_RANGE_MAX=100
PRIMER_WT_TM_LT=1.0
PRIMER_WT_TM_GT=1.0
PRIMER_WT_SIZE_LT=1.0
PRIMER_WT_SIZE_GT=1.0
PRIMER_WT_GC_PERCENT_LT=0.0
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_COMPL_ANY=0.0
PRIMER_WT_COMPL_END=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_REP_SIM=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY=0.0
PRIMER_PAIR_WT_COMPL_END=0.0
PRIMER_PAIR_WT_REP_SIM=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_INTERNAL_OLIGO_MIN_SIZE=18
PRIMER_INTERNAL_OLIGO_OPT_SIZE=20
PRIMER_INTERNAL_OLIGO_MAX_SIZE=27
PRIMER_INTERNAL_OLIGO_MIN_TM=57.0
PRIMER_INTERNAL_OLIGO_OPT_TM=60.0
PRIMER_INTERNAL_OLIGO_MAX_TM=63.0
PRIMER_INTERNAL_OLIGO_MIN_GC=20.0
PRIMER_INTERNAL_OLIGO_MAX_GC=80.0
PRIMER_INTERNAL_OLIGO_SELF_ANY=12.00
PRIMER_INTERNAL_OLIGO_SELF_END=12.00
PRIMER_INTERNAL_OLIGO_NUM_NS=0
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=5
PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=
PRIMER_INTERNAL_OLIGO_MAX_MISHYB=12.00
PRIMER_INTERNAL_OLIGO_MIN_QUALITY=0
PRIMER_INTERNAL_OLIGO_SALT_CONC=50.0
PRIMER_INTERNAL_OLIGO_DNA_CONC=50.0
PRIMER_INTERNAL_OLIGO_DIVALENT_CONC=0.0
PRIMER_INTERNAL_OLIGO_DNTP_CONC=0.0
PRIMER_IO_WT_TM_LT=1.0
PRIMER_IO_WT_TM_GT=1.0
PRIMER_IO_WT_SIZE_LT=1.0
PRIMER_IO_WT_SIZE_GT=1.0
PRIMER_IO_WT_GC_PERCENT_LT=0.0
PRIMER_IO_WT_GC_PERCENT_GT=0.0
PRIMER_IO_WT_COMPL_ANY=0.0
PRIMER_IO_WT_NUM_NS=0.0
PRIMER_IO_WT_REP_SIM=0.0
PRIMER_IO_WT_SEQ_QUAL=0.0
PRIMER_TASK=pick_left_only
PRIMER_FIRST_BASE_INDEX=1
PRIMER_PICK_ANYWAY=1
COMMENT=Test for very short target of alignment number 1
=
